FastQCFastQC Report
Thu 26 May 2016
SRR2132685_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132685_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3518219
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2596487.380097714212789No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1910485.430247520123108No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG331880.9433181959394796No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT103090.29301757508557597No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA81210.23082701787466897No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT55490.15772184733241448No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT46030.1308332426150845No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37590.1068438320638937No Hit
CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT35230.10013589262066971No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT29300.082.86551
GTGGAAT918100.064.820226
AACGAGT841200.064.365711
ACGAGTG887900.064.029792
TGGAATC935000.063.965247
AGTGGAA912350.063.8586585
GAATCAC937350.063.7346959
ATCGAGT10350.063.6472051
GGAATCA940850.063.5675248
CGAGTGG912050.063.189243
GAGTGGA939500.062.0882724
AACGTGT14750.059.654231
TCGAGTG13350.058.8240622
CGTGTGG20700.056.9813843
ACGTGTG19400.053.083822
CGATTGG6150.044.3182373
AGTACTT399700.040.79139720-21
GAGTACT393850.040.4904720-21
GTACTTT396750.039.5850822-23
TACTTTT415850.038.7500922-23