##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132685_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3518219
Sequences flagged as poor quality	0
Sequence length	100
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.19036137318342	31.0	28.0	34.0	16.0	34.0
2	30.307789253596777	33.0	30.0	34.0	22.0	34.0
3	30.743911052722982	33.0	31.0	34.0	25.0	34.0
4	35.16068044655549	37.0	35.0	37.0	32.0	37.0
5	34.61558447612272	37.0	35.0	37.0	30.0	37.0
6	35.137934847148514	37.0	35.0	37.0	32.0	37.0
7	35.51349248014407	37.0	35.0	37.0	33.0	37.0
8	35.710269315241604	37.0	35.0	37.0	35.0	37.0
9	37.605095930639905	39.0	38.0	39.0	35.0	39.0
10-11	37.68123644378022	39.0	39.0	39.0	35.0	39.0
12-13	37.65172534739878	39.0	39.0	39.0	35.0	39.0
14-15	39.0476438504823	41.0	39.0	41.0	36.0	41.0
16-17	38.937071853685055	41.0	39.0	41.0	36.0	41.0
18-19	38.694954464176334	41.0	38.5	41.0	35.5	41.0
20-21	38.487914623848035	40.5	38.5	41.0	35.0	41.0
22-23	38.36853760382739	40.0	38.0	41.0	35.0	41.0
24-25	38.27536190896588	40.0	38.0	41.0	35.0	41.0
26-27	38.21312757960775	40.0	38.0	41.0	34.5	41.0
28-29	38.03487162112421	40.0	38.0	41.0	34.0	41.0
30-31	37.89853729969624	40.0	38.0	41.0	34.0	41.0
32-33	37.747515433234824	40.0	38.0	41.0	34.0	41.0
34-35	37.65300113494925	40.0	38.0	41.0	33.0	41.0
36-37	37.47760216177561	40.0	37.0	41.0	33.0	41.0
38-39	37.03184281592476	40.0	36.0	41.0	33.0	41.0
40-41	36.83237115142633	40.0	35.0	41.0	33.0	41.0
42-43	36.69023687837511	40.0	35.0	41.0	33.0	41.0
44-45	36.53979627192054	39.5	35.0	41.0	33.0	41.0
46-47	36.39196010822521	39.0	35.0	41.0	32.0	41.0
48-49	36.21952087121353	39.0	35.0	41.0	31.0	41.0
50-51	35.95274583532179	39.0	35.0	41.0	31.0	41.0
52-53	35.47288315480077	39.0	35.0	41.0	29.0	41.0
54-55	34.67651331540191	38.0	35.0	41.0	24.5	41.0
56-57	34.060421338182756	38.0	35.0	40.5	18.5	41.0
58-59	33.748469751314516	38.0	35.0	40.0	13.0	41.0
60-61	33.49702562574985	37.0	34.0	40.0	9.5	41.0
62-63	33.204131550651056	37.0	34.0	40.0	7.5	41.0
64-65	32.922610559490465	36.0	34.0	40.0	7.0	41.0
66-67	32.588180127502014	35.5	33.5	39.0	3.5	41.0
68-69	32.29826426382212	35.0	33.0	39.0	2.0	41.0
70-71	31.97997466900156	35.0	33.0	38.5	2.0	40.5
72-73	31.647810866805052	35.0	33.0	37.5	2.0	40.0
74-75	31.314290554396983	35.0	33.0	37.0	2.0	39.5
76-77	30.963267352032375	35.0	33.0	36.5	2.0	39.0
78-79	30.842470721691857	35.0	33.0	36.0	2.0	39.0
80-81	30.705548318623713	35.0	33.0	36.0	2.0	38.5
82-83	30.40693174586346	35.0	33.0	35.5	2.0	37.0
84-85	30.14550458626936	35.0	33.0	35.0	2.0	37.0
86-87	29.980960679252767	35.0	33.0	35.0	2.0	36.5
88-89	29.788683564041918	35.0	33.0	35.0	2.0	36.0
90-91	29.596816457417802	35.0	33.0	35.0	2.0	36.0
92-93	29.442606614312524	35.0	32.0	35.0	2.0	36.0
94-95	29.263729887195765	35.0	32.0	35.0	2.0	35.5
96-97	29.074309757294813	35.0	32.0	35.0	2.0	35.0
98-99	28.92870654157686	35.0	32.0	35.0	2.0	35.0
100	28.827065341867577	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	2.7937068937815646
1101	2	2.2736017512906024
1101	3	2.094749005916782
1101	4	1.0296026920108332
1101	5	1.5815552884339539
1101	6	0.9927398708403103
1101	7	0.5213917967704091
1101	8	0.36716958914914954
1101	9	0.3074957593400214
1101	10-11	0.31382176274089346
1101	12-13	0.2572426634663927
1101	14-15	0.44603299596160184
1101	16-17	0.39628043704095006
1101	18-19	0.48044168215731986
1101	20-21	0.5197713796249985
1101	22-23	0.49301602950571777
1101	24-25	0.5422630487035818
1101	26-27	0.4934579285581506
1101	28-29	0.49052733651279823
1101	30-31	0.5242412189722856
1101	32-33	0.5185375962112744
1101	34-35	0.45833089397702764
1101	36-37	0.5197368585024407
1101	38-39	0.5284611943369057
1101	40-41	0.5322540279029226
1101	42-43	0.4683360975342836
1101	44-45	0.5160130410097565
1101	46-47	0.47609389672623337
1101	48-49	0.4140052792004596
1101	50-51	0.4450259532756107
1101	52-53	0.4433985078228915
1101	54-55	0.48476645486147163
1101	56-57	0.40467341976426496
1101	58-59	0.31857785192257637
1101	60-61	0.3377676955553923
1101	62-63	0.38499705560334974
1101	64-65	0.33907522224637887
1101	66-67	0.3693947061458829
1101	68-69	0.35377306841864
1101	70-71	0.3379950100843878
1101	72-73	0.3281524117252488
1101	74-75	0.3289875576117325
1101	76-77	0.2982255113074572
1101	78-79	0.3915435727086951
1101	80-81	0.4060871135716937
1101	82-83	0.5438642799370363
1101	84-85	0.5468816609207039
1101	86-87	0.46679230752972956
1101	88-89	0.4605297898360732
1101	90-91	0.4174340443731417
1101	92-93	0.41825893485237486
1101	94-95	0.4944081634360664
1101	96-97	0.5119431191103878
1101	98-99	0.5774202560642365
1101	100	0.5564071948437928
1102	1	2.7344240694140574
1102	2	2.2521372444210606
1102	3	2.0367115847342276
1102	4	1.0635184118333
1102	5	1.484867133232683
1102	6	0.9161637012508024
1102	7	0.38480882229116276
1102	8	0.30008200873921
1102	9	0.2775857282706582
1102	10-11	0.23410515923459485
1102	12-13	0.23410223101396355
1102	14-15	0.32466929942599876
1102	16-17	0.3398894894626139
1102	18-19	0.36936785532719085
1102	20-21	0.39184994103001003
1102	22-23	0.3784735137349031
1102	24-25	0.38906401468454277
1102	26-27	0.31818627478250505
1102	28-29	0.43400349809224537
1102	30-31	0.4379968745209766
1102	32-33	0.4360017482659302
1102	34-35	0.3248380891924256
1102	36-37	0.3622515456861777
1102	38-39	0.3044268751121635
1102	40-41	0.3384224107773264
1102	42-43	0.38116730141695854
1102	44-45	0.37999563970445394
1102	46-47	0.3192127064108732
1102	48-49	0.3417104278822549
1102	50-51	0.3337445348419621
1102	52-53	0.3074560452377071
1102	54-55	0.303084648401196
1102	56-57	0.3075601419995593
1102	58-59	0.3372998286119895
1102	60-61	0.3170009571301122
1102	62-63	0.33501781659415286
1102	64-65	0.2823689975514654
1102	66-67	0.23909150122879197
1102	68-69	0.22911719346461012
1102	70-71	0.2508987547945658
1102	72-73	0.2712367100608688
1102	74-75	0.2711540671279593
1102	76-77	0.1734061037384933
1102	78-79	0.2817698429804061
1102	80-81	0.3875122869484784
1102	82-83	0.4210166722723905
1102	84-85	0.37882861572888515
1102	86-87	0.4074798115292637
1102	88-89	0.43935160232658177
1102	90-91	0.34899884831778394
1102	92-93	0.4341084192432305
1102	94-95	0.4213663960274019
1102	96-97	0.3867833389228217
1102	98-99	0.445798100385872
1102	100	0.4945383439739679
1103	1	2.56308858455888
1103	2	2.125881394719901
1103	3	1.951327151609
1103	4	0.9874692471481623
1103	5	1.5139957711910554
1103	6	0.9320657610832583
1103	7	0.47299334083432854
1103	8	0.3954287471787623
1103	9	0.4186945341114523
1103	10-11	0.31347191119004236
1103	12-13	0.28366889138048634
1103	14-15	0.4336201404813167
1103	16-17	0.42346053137351447
1103	18-19	0.4796893192690348
1103	20-21	0.5917909471104466
1103	22-23	0.519925760124913
1103	24-25	0.6453261853421921
1103	26-27	0.6445870960404321
1103	28-29	0.6917876063939374
1103	30-31	0.7026524128886606
1103	32-33	0.7284316799358805
1103	34-35	0.691188177042882
1103	36-37	0.7036875286548323
1103	38-39	0.6822052544415982
1103	40-41	0.6564300616533032
1103	42-43	0.705605354105991
1103	44-45	0.6447127623711921
1103	46-47	0.6612446919288644
1103	48-49	0.5934947759913669
1103	50-51	0.5862537766127716
1103	52-53	0.5515342655135314
1103	54-55	0.4798545485072694
1103	56-57	0.47562437580477024
1103	58-59	0.45130949783128926
1103	60-61	0.47269643383958737
1103	62-63	0.4302589845123066
1103	64-65	0.403019492069987
1103	66-67	0.3974713573472073
1103	68-69	0.3918381417128458
1103	70-71	0.3805598413102267
1103	72-73	0.3787989713746214
1103	74-75	0.32502830972898167
1103	76-77	0.3752696593378708
1103	78-79	0.35216771091869603
1103	80-81	0.3410700296645288
1103	82-83	0.4025018272452563
1103	84-85	0.3917123991065736
1103	86-87	0.42490727121657557
1103	88-89	0.5056749324897503
1103	90-91	0.48972038883727365
1103	92-93	0.5120994715988907
1103	94-95	0.5613388971040969
1103	96-97	0.5168314037371324
1103	98-99	0.5015543684943111
1103	100	0.5717767152868589
1104	1	1.553790940683573
1104	2	1.721350301353528
1104	3	1.8039121865262544
1104	4	0.9178291477586953
1104	5	1.563041576218538
1104	6	1.0485367827617438
1104	7	0.5202042866360372
1104	8	0.3998601291788617
1104	9	0.32266757556536163
1104	10-11	0.2666300949405169
1104	12-13	0.2492272838170635
1104	14-15	0.38694162821397526
1104	16-17	0.3678684224805835
1104	18-19	0.43537904859066856
1104	20-21	0.5578652037188832
1104	22-23	0.4241419477489998
1104	24-25	0.48072135883975164
1104	26-27	0.4448961436433194
1104	28-29	0.5348279153831967
1104	30-31	0.47754440981277213
1104	32-33	0.4798009453462839
1104	34-35	0.4345806912895469
1104	36-37	0.45074492935444965
1104	38-39	0.40424778488512203
1104	40-41	0.4087941389380063
1104	42-43	0.43023210769100473
1104	44-45	0.46908693772473953
1104	46-47	0.48152667401398475
1104	48-49	0.43718582341193013
1104	50-51	0.37498532039872856
1104	52-53	0.4662860023446811
1104	54-55	0.3527369646499494
1104	56-57	0.2707891195468477
1104	58-59	0.2724170664138654
1104	60-61	0.28204232038903854
1104	62-63	0.30385216129451464
1104	64-65	0.371810315869169
1104	66-67	0.4078799743434516
1104	68-69	0.3496170339544946
1104	70-71	0.3586426511536196
1104	72-73	0.35593753840554854
1104	74-75	0.4743098319484105
1104	76-77	0.44794541353553896
1104	78-79	0.3983686811559046
1104	80-81	0.4181094300272079
1104	82-83	0.4434865407695874
1104	84-85	0.4292293014377151
1104	86-87	0.44095147455072947
1104	88-89	0.4338416855701226
1104	90-91	0.39825629536624874
1104	92-93	0.38352972280588915
1104	94-95	0.4292306493709326
1104	96-97	0.5020071658417073
1104	98-99	0.4741078783983603
1104	100	0.46913887980295144
1105	1	2.2101934034840376
1105	2	2.0339566151241506
1105	3	1.9614122971720747
1105	4	1.0023866460384383
1105	5	1.6028755173180684
1105	6	1.1032847825158854
1105	7	0.5876446526682457
1105	8	0.472065630440909
1105	9	0.377568483370446
1105	10-11	0.28369932081692184
1105	12-13	0.23555132264903733
1105	14-15	0.37039981982142933
1105	16-17	0.36073004623536775
1105	18-19	0.38417935535664327
1105	20-21	0.4674107305681616
1105	22-23	0.4402972012848565
1105	24-25	0.5025694173136159
1105	26-27	0.40363118899158223
1105	28-29	0.47288542491681085
1105	30-31	0.5428820406320938
1105	32-33	0.5569418180916088
1105	34-35	0.5190008029072644
1105	36-37	0.5198070396204599
1105	38-39	0.5255739048221599
1105	40-41	0.480910366402135
1105	42-43	0.5483950305137668
1105	44-45	0.6279567851312464
1105	46-47	0.6958583329875268
1105	48-49	0.5978501464291739
1105	50-51	0.5792917280341783
1105	52-53	0.6219788864383204
1105	54-55	0.5632573827101339
1105	56-57	0.6179499167997164
1105	58-59	0.5133445048498757
1105	60-61	0.5668107994285236
1105	62-63	0.5915740233738589
1105	64-65	0.5706116895155304
1105	66-67	0.5210494495325975
1105	68-69	0.5155404922383369
1105	70-71	0.5334940054619075
1105	72-73	0.5278660706348646
1105	74-75	0.5279510981661097
1105	76-77	0.48879777800748414
1105	78-79	0.48717153742185815
1105	80-81	0.5438902402079151
1105	82-83	0.6453313456024041
1105	84-85	0.6287053048542148
1105	86-87	0.5393673361299527
1105	88-89	0.5716632046026824
1105	90-91	0.6378986419552142
1105	92-93	0.6382841376085722
1105	94-95	0.6886877352773162
1105	96-97	0.6605526299859612
1105	98-99	0.6645521542374624
1105	100	0.6442069312849554
1106	1	1.6282725815592975
1106	2	1.1224071840607515
1106	3	1.520821647160119
1106	4	0.7982453876267641
1106	5	1.479684260117459
1106	6	1.0575492544321037
1106	7	0.625740140558257
1106	8	0.4199559529585315
1106	9	0.3173154738228874
1106	10-11	0.26386529205046827
1106	12-13	0.20367099089659746
1106	14-15	0.3166689630784276
1106	16-17	0.31004092043099973
1106	18-19	0.3432229756805256
1106	20-21	0.4013345698353916
1106	22-23	0.3555217268676998
1106	24-25	0.43455350534164694
1106	26-27	0.38800207615959437
1106	28-29	0.45407044175319555
1106	30-31	0.4576722411204983
1106	32-33	0.4412307380026306
1106	34-35	0.457915608747804
1106	36-37	0.41789035202233293
1106	38-39	0.4102599649755163
1106	40-41	0.37274070217583244
1106	42-43	0.41106309165978416
1106	44-45	0.397580501191662
1106	46-47	0.3813566488131457
1106	48-49	0.29219153194178915
1106	50-51	0.25642753360427406
1106	52-53	0.3516458479966431
1106	54-55	0.25839025524346937
1106	56-57	0.2251999292113993
1106	58-59	0.2316320820247526
1106	60-61	0.27335053934666576
1106	62-63	0.33194229358471716
1106	64-65	0.3132976482343537
1106	66-67	0.296942317548222
1106	68-69	0.3703587357590763
1106	70-71	0.3691066325076413
1106	72-73	0.3292856621642031
1106	74-75	0.28463682751980457
1106	76-77	0.13676568856739735
1106	78-79	0.11630340511109338
1106	80-81	0.14352180740741716
1106	82-83	0.17180714430224597
1106	84-85	0.18631526261971487
1106	86-87	0.17364009744127173
1106	88-89	0.1341112077008546
1106	90-91	0.0656203556740742
1106	92-93	0.13157268162305868
1106	94-95	0.1655294677147232
1106	96-97	0.1555455549949336
1106	98-99	0.10954227574612219
1106	100	0.09010385801965626
1107	1	1.4963463625036084
1107	2	0.9978930910442472
1107	3	1.3918326784419008
1107	4	0.7238118878833077
1107	5	1.4752189174448915
1107	6	1.0535278179143077
1107	7	0.5862022286807971
1107	8	0.48548726850219026
1107	9	0.4348150529984167
1107	10-11	0.2583230435588746
1107	12-13	0.1263001591972568
1107	14-15	0.20261025241669728
1107	16-17	0.20073282507406986
1107	18-19	0.26262221006076203
1107	20-21	0.24581638897763725
1107	22-23	0.22746196731159074
1107	24-25	0.28293787564002315
1107	26-27	0.23556903818040098
1107	28-29	0.14732353505792162
1107	30-31	0.024423898659620136
1107	32-33	9.198567764912013E-5
1107	34-35	-0.04015316931505453
1107	36-37	0.0754310858070113
1107	38-39	-0.010255352055821731
1107	40-41	0.06734092349881138
1107	42-43	0.05868188462942214
1107	44-45	0.05964459200774286
1107	46-47	0.024955017333148533
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2214	4	-1.3918746915796092
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2214	96-97	-0.43386840573882424
2214	98-99	-0.42178782487332
2214	100	-0.4334690721522598
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2215	3	-3.6461038031009068
2215	4	-1.8981812989259126
2215	5	-2.677262937561224
2215	6	-4.9782342887881335
2215	7	-2.084430500216051
2215	8	-1.1651622072630232
2215	9	-1.5307220619383486
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2215	24-25	-0.8659218354345697
2215	26-27	-0.9068989700368775
2215	28-29	-0.9715170725048736
2215	30-31	-1.0525082851268763
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2215	100	-0.7499285630919026
2216	1	-5.785846911861263
2216	2	-4.6577595624039745
2216	3	-5.9077484810998655
2216	4	-3.0616927284984357
2216	5	-4.104072290372542
2216	6	-1.9211057910089906
2216	7	-1.2629492303674894
2216	8	-0.7940727787231623
2216	9	-0.6599558349282617
2216	10-11	-0.8832009589545393
2216	12-13	-0.6264787351756951
2216	14-15	-1.489455079515949
2216	16-17	-0.9834005002646435
2216	18-19	-1.4799684416616827
2216	20-21	-1.0104390933007252
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2216	24-25	-1.455476863089963
2216	26-27	-1.2775732870173897
2216	28-29	-1.960490918027645
2216	30-31	-2.016473974384631
2216	32-33	-1.284602665821268
2216	34-35	-1.2697855555921294
2216	36-37	-1.2906945573569075
2216	38-39	-1.194047631498698
2216	40-41	-1.2194719447354103
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2216	46-47	-1.6702772130488839
2216	48-49	-1.3989769717227674
2216	50-51	-1.274659799144878
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2216	54-55	-1.038078666902365
2216	56-57	-0.9449341890173031
2216	58-59	-0.9736141750629699
2216	60-61	-1.020576889860962
2216	62-63	-1.037821112694715
2216	64-65	-1.0770979024010074
2216	66-67	-1.1386204750389624
2216	68-69	-1.1768292586159177
2216	70-71	-1.1678180036309946
2216	72-73	-1.143251383708872
2216	74-75	-1.092553033828672
2216	76-77	-1.1656898332530652
2216	78-79	-1.1242043897769598
2216	80-81	-1.0872245836808219
2216	82-83	-1.0788456462619287
2216	84-85	-1.0878458927795478
2216	86-87	-1.1212242403812809
2216	88-89	-1.0900094464187653
2216	90-91	-1.143117477174858
2216	92-93	-1.1967236900152614
2216	94-95	-1.1879051407176995
2216	96-97	-1.1894921211869978
2216	98-99	-1.185600260288247
2216	100	-1.2946528235964863
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	8817.0
3	8089.0
4	8993.0
5	11901.0
6	10093.0
7	6956.0
8	6374.0
9	6493.0
10	6398.0
11	11305.0
12	7508.0
13	9403.0
14	8728.0
15	9192.0
16	10732.0
17	13144.0
18	17901.0
19	28020.0
20	56271.0
21	74415.0
22	35266.0
23	23211.0
24	24034.0
25	27640.0
26	27591.0
27	29579.0
28	33873.0
29	39219.0
30	45673.0
31	56112.0
32	72554.0
33	101248.0
34	150049.0
35	376898.0
36	430080.0
37	683012.0
38	782678.0
39	256716.0
40	2053.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	14.772618360658907	44.21131904188095	25.056645263710823	15.959417333749315
2	17.29498605451793	42.633298386670134	23.263868544419335	16.807847014392603
3	18.27626940684176	26.101026144485644	20.530478782156194	35.092225666516406
4	33.60833990152404	27.332238271693715	21.533110929137724	17.526310897644517
5	16.91105641803424	41.59073099201613	22.933137476660775	18.565075113288852
6	36.001454144838625	25.075101919465503	22.688098722677584	16.23534521301829
7	18.046176204494376	25.163584188477184	39.2602052345235	17.53003437250495
8	35.13345815027433	25.627881607142704	22.089699362092013	17.14896088049095
9	34.9101917760094	26.863904719973377	21.994054378081636	16.231849125935593
10-11	16.411442835139027	44.67143745173339	22.133073580695232	16.784046132432348
12-13	16.350431852025128	26.54057351176831	30.92873126999769	26.180263366208866
14-15	16.680101494534593	35.298413771285986	21.987886484610534	26.033598249568886
16-17	26.02348377366771	26.232112207989474	30.773333591039666	16.971070427303154
18-19	17.055859797244004	26.521316609341262	30.75600182933467	25.666821764080066
20-21	26.170880840974064	35.571481601702686	21.809780325540913	16.447857231782333
22-23	25.77179330959689	26.702207381377818	30.797319723035965	16.72867958598933
24-25	16.879392669984444	35.532623182354484	21.74183869736364	25.846145450297435
26-27	17.26192261158713	30.471813700235906	35.5834260552426	16.682837632934366
28-29	25.494089571108763	26.5455683437042	31.359296319582697	16.60104576560434
30-31	23.1030184196123	27.91547452946852	31.72078641473114	17.260720636188033
32-33	19.275353173767375	29.191093435249982	32.675022149305505	18.858531241677134
34-35	19.495733756018623	28.979505230247277	32.85709230285365	18.66766871088045
36-37	19.51185851927338	28.773300245740753	33.34490262042931	18.36993861455655
38-39	20.273016169018103	28.707533194969436	32.77242986124476	18.2470207747677
40-41	18.716330537567323	30.131924246418716	32.86210594160087	18.28963927441309
42-43	18.886212312588928	28.80582927334341	33.47112118826533	18.836837225802334
44-45	18.711249264174814	29.553439739363686	32.6711498826517	19.064161113809806
46-47	19.56032473959266	28.8112020492635	33.12313715591923	18.505336055224607
48-49	18.861301357695005	29.07080394361153	33.083216911425076	18.984677787268396
50-51	19.541964205258207	29.78557323123428	32.25109874069435	18.42136382281316
52-53	19.642019891131593	29.39044805570506	32.25520419328555	18.712327859877796
54-55	19.495893312534605	30.516243699838892	30.499274621649246	19.488588365977254
56-57	19.897396366720585	31.180754698639845	29.59094980130459	19.33089913333498
58-59	21.311391536520308	31.169837073731028	28.370326587291494	19.148444802457178
60-61	20.671914355713614	31.843681894762156	28.11611620602997	19.368287543494258
62-63	19.918926347558482	31.789339253854077	28.085086546685357	20.206647851902087
64-65	20.289991227805864	31.70410530726273	28.586835341424827	19.41906812350658
66-67	20.29585429608711	30.947939314577827	29.398994526907302	19.357211862427768
68-69	20.715638727918247	30.894561201952865	29.101626765641164	19.288173304487724
70-71	19.238786538445137	32.02604132614338	29.265592531844554	19.46957960356693
72-73	19.219598342615406	30.872994960593488	29.798244664053303	20.10916203273781
74-75	19.38951188010069	31.5736358178953	28.93409665074164	20.102755651262367
76-77	20.277000042637255	30.91402907860889	29.12572305680704	19.683247821946818
78-79	19.915619407195205	31.34257733217517	28.509806379772083	20.23199688085754
80-81	20.57370541484515	32.017326799516646	27.848891797187132	19.560075988451075
82-83	20.54064049000652	31.782160515339143	28.185508559707607	19.491690434946733
84-85	20.172984714713934	32.374082110392486	27.326067674012627	20.126865500880957
86-87	20.16900395541172	32.41832991516402	27.787115562548237	19.625550566876022
88-89	21.475620224668003	31.78611629070402	27.195672071129707	19.542591413498272
90-91	20.789681076122033	32.5092435266888	27.105282045706335	19.595793351482836
92-93	20.20260438149422	32.573970796292386	26.983766463578085	20.23965835863531
94-95	20.555138720721814	32.78784427189583	26.841833945035887	19.815183062346474
96-97	20.640051849998862	32.05465569781457	27.38622279580652	19.91906965638005
98-99	21.24794386564912	32.23084927764202	26.5202887555921	20.000918101116756
100	20.07748467574255	33.406355392021005	26.541031205631427	19.975128726605014
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	192820.0
1	103958.0
2	10911.0
3	5562.0
4	4141.0
5	3693.0
6	3655.5
7	4177.5
8	3952.0
9	3441.0
10	3681.5
11	5578.0
12	46428.5
13	50435.5
14	12990.0
15	10922.5
16	11751.5
17	13186.0
18	14608.0
19	15737.0
20	17059.0
21	18541.0
22	20411.5
23	22913.0
24	25845.5
25	29122.5
26	32867.0
27	36776.0
28	40025.0
29	42637.0
30	46325.0
31	50487.5
32	54251.0
33	58485.0
34	63356.0
35	67809.5
36	71643.5
37	75214.0
38	79408.5
39	82175.5
40	83600.0
41	87049.0
42	89053.5
43	88939.0
44	89277.0
45	90355.5
46	92770.5
47	98072.0
48	102413.0
49	113782.0
50	153583.5
51	152920.5
52	108996.0
53	93254.0
54	90031.5
55	87788.0
56	82746.0
57	75258.5
58	68297.0
59	59596.0
60	50213.0
61	42262.5
62	35777.0
63	28724.0
64	22241.5
65	17373.0
66	13271.5
67	10486.0
68	8295.5
69	6076.0
70	4210.5
71	3063.5
72	2244.0
73	1593.0
74	1231.5
75	894.0
76	642.0
77	485.5
78	322.5
79	184.5
80	114.0
81	67.0
82	36.0
83	19.5
84	12.0
85	9.0
86	5.5
87	2.5
88	1.5
89	2.0
90	1.0
91	0.0
92	1.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05792703637834939
2	0.067789981237666
3	2.842347221705073E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	7.105868054262682E-5
18-19	0.0
20-21	3.6950513882165947E-4
22-23	1.4211736108525364E-5
24-25	0.0
26-27	0.0023449364579066854
28-29	8.811276387285726E-4
30-31	0.005755753123952773
32-33	0.0014211736108525365
34-35	0.0022028190968214315
36-37	0.008072266109642406
38-39	0.012563174719936423
40-41	0.002884982430030649
42-43	0.007120079790371208
44-45	0.0017054083330230439
46-47	0.002188607360712906
48-49	0.0035245105549142905
50-51	0.00932289888719264
52-53	0.0033681814577205113
54-55	0.0015632909719377902
56-57	5.684694443410146E-4
58-59	9.237628470541488E-4
60-61	0.006423704721053465
62-63	0.0017054083330230439
64-65	0.008100689581859458
66-67	0.010587743400851397
68-69	0.011667835345099324
70-71	0.009919791803750706
72-73	0.004149826943689406
74-75	0.001904372638542399
76-77	0.0048035668046815735
78-79	0.0166419429830832
80-81	0.00898181722058803
82-83	0.006395281248836414
84-85	0.004618814235270744
86-87	0.007219561943130885
88-89	0.003950862638170051
90-91	0.006153681734991483
92-93	0.010744072498045177
94-95	0.008683370762308998
96-97	0.010772495970262226
98-99	0.002501265555100464
100	0.0029560411105732758
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3518219.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.895404694428464
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.56882849039982	42.883942125516676
2	13.510854848349357	14.563459796389392
3	3.6779654416546443	5.946763077902981
4	1.1814908074766555	2.5470770084680563
5	0.5216527578088347	1.405734324603591
6	0.3348463069979052	1.082800633565216
7	0.23399368646646318	0.882782909963587
8	0.16897040353897605	0.7285382624091201
9	0.11963482760546261	0.5802990704410129
>10	0.5679068816015872	5.498577808627009
>50	0.09179307554551884	3.265259732859971
>100	0.016261707123183398	1.7182408536171423
>500	0.0018626311021949962	0.7224727479012044
>1k	0.003618826140549137	3.6074877863920434
>5k	1.0643606295732754E-4	0.39208394386785317
>10k+	2.1287212591465508E-4	14.174479917475196
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	259648	7.380097714212789	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	191048	5.430247520123108	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	33188	0.9433181959394796	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	10309	0.29301757508557597	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8121	0.23082701787466897	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5549	0.15772184733241448	No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4603	0.1308332426150845	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3759	0.1068438320638937	No Hit
CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3523	0.10013589262066971	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.005201495415720283	0.0	0.0	0.0	0.0
2	0.005201495415720283	0.0	0.0	0.0	0.0
3	0.005201495415720283	0.0	0.0	0.0	0.0
4	0.005201495415720283	0.0	0.0	0.0	0.0
5	0.005229918887937334	0.0	0.0	0.0	0.0
6	0.005229918887937334	0.0	0.0	0.0	0.0
7	0.005229918887937334	0.0	0.0	0.0	0.0
8	0.005229918887937334	0.0	0.0	0.0	0.0
9	0.005229918887937334	0.0	0.0	0.0	0.0
10-11	0.005229918887937334	0.0	0.0	0.0	0.0
12-13	0.005258342360154385	0.0	0.0	0.0	0.0
14-15	0.00542888319345669	0.0	0.0	0.0	0.0
16-17	0.005528365346216367	0.0	0.0	0.0	0.0
18-19	0.005599424026758994	0.0	0.0	0.0	0.0
20-21	0.005670482707301621	0.0	0.0	0.0	0.0
22-23	0.0057273296517357224	0.0	0.0	0.0	0.0
24-25	0.005841023540603925	0.0	0.0	0.0	0.0
26-27	0.006068411318340331	0.0	0.0	0.0	0.0
28-29	0.006167893471100009	0.0	0.0	0.0	0.0
30-31	0.006409492984944939	0.0	0.0	0.0	0.0
32-33	0.006679515971006921	0.0	0.0	0.0	0.0
34-35	0.007006385901503005	0.0	0.0	0.0	0.0
36-37	0.007319044095890563	0.0	0.0	0.0	0.0
38-39	0.00802963090131683	0.0	0.0	0.0	1.4211736108525364E-5
40-41	0.008811276387285726	0.0	0.0	0.0	2.842347221705073E-5
42-43	0.009578710137146096	0.0	0.0	0.0	2.842347221705073E-5
44-45	0.010658802081394024	0.0	0.0	0.0	2.842347221705073E-5
46-47	0.01207997569224656	0.0	0.0	0.0	2.842347221705073E-5
48-49	0.013586419719750248	0.0	0.0	0.0	2.842347221705073E-5
50-51	0.015661333191594955	0.0	0.0	0.0	2.842347221705073E-5
52-53	0.018290504371672144	0.0	0.0	0.0	2.842347221705073E-5
54-55	0.021360239371113622	0.0	0.0	0.0	2.842347221705073E-5
56-57	0.025495854578694507	0.0	0.0	0.0	2.842347221705073E-5
58-59	0.03075419693884889	0.0	0.0	0.0	2.842347221705073E-5
60-61	0.03663785568777839	0.0	0.0	0.0	2.842347221705073E-5
62-63	0.045150685616785084	0.0	0.0	0.0	2.842347221705073E-5
64-65	0.054416737559543626	0.0	0.0	0.0	2.842347221705073E-5
66-67	0.06713624137667382	0.0	0.0	0.0	2.842347221705073E-5
68-69	0.08230016380447039	0.0	0.0	0.0	2.842347221705073E-5
70-71	0.10353249755060728	0.0	0.0	0.0	2.842347221705073E-5
72-73	0.12926995164314672	0.0	0.0	0.0	2.842347221705073E-5
74-75	0.1624401437204449	0.0	0.0	0.0	2.842347221705073E-5
76-77	0.20392420142123047	0.0	0.0	0.0	2.842347221705073E-5
78-79	0.2567350128005107	0.0	0.0	0.0	2.842347221705073E-5
80-81	0.32010514410842533	0.0	0.0	0.0	2.842347221705073E-5
82-83	0.40385490499596527	0.0	0.0	0.0	2.842347221705073E-5
84-85	0.5086238235880143	0.0	0.0	0.0	2.842347221705073E-5
86-87	0.633161267107022	0.0	0.0	1.4211736108525364E-5	2.842347221705073E-5
88	0.7426769055593185	0.0	0.0	5.684694443410146E-5	2.842347221705073E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	2930	0.0	82.8655	1
GTGGAAT	91810	0.0	64.82022	6
AACGAGT	84120	0.0	64.36571	1
ACGAGTG	88790	0.0	64.02979	2
TGGAATC	93500	0.0	63.96524	7
AGTGGAA	91235	0.0	63.858658	5
GAATCAC	93735	0.0	63.734695	9
ATCGAGT	1035	0.0	63.647205	1
GGAATCA	94085	0.0	63.567524	8
CGAGTGG	91205	0.0	63.18924	3
GAGTGGA	93950	0.0	62.088272	4
AACGTGT	1475	0.0	59.65423	1
TCGAGTG	1335	0.0	58.824062	2
CGTGTGG	2070	0.0	56.981384	3
ACGTGTG	1940	0.0	53.08382	2
CGATTGG	615	0.0	44.318237	3
AGTACTT	39970	0.0	40.791397	20-21
GAGTACT	39385	0.0	40.49047	20-21
GTACTTT	39675	0.0	39.58508	22-23
TACTTTT	41585	0.0	38.75009	22-23
>>END_MODULE