FastQCFastQC Report
Thu 26 May 2016
SRR2132684_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132684_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3716535
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2365596.365041631519682No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1093422.942041444517541No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG202910.5459655297205596No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT92630.2492375290425087No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT48650.1309014983042No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46300.12457840434705982No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT43610.11734047977484405No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT24950.080.531661
AACGAGT676950.072.1731341
ACGAGTG715600.071.382142
GTGGAAT753450.071.137836
GAATCAC765650.070.611979
CGAGTGG734050.070.5082243
TGGAATC766700.070.331387
GGAATCA770350.070.162868
AGTGGAA747950.069.939315
GAGTGGA759300.068.980524
AACGTGT16900.057.9143181
CGTGTGG20850.057.2515753
ACGTGTG20650.053.73562
ATCGAGT11150.053.5967181
TCGAGTG12400.053.4647562
GAGTACT356950.041.51911520-21
AGTACTT365100.041.35175720-21
GTACTTT362300.040.23176222-23
TACTTTT384050.038.79154622-23
CGATTGG7350.037.7245833