##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132684_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3716535
Sequences flagged as poor quality	0
Sequence length	100
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.262906443770877	31.0	28.0	34.0	16.0	34.0
2	30.378405961466797	33.0	30.0	34.0	23.0	34.0
3	30.833832857755947	33.0	31.0	34.0	25.0	34.0
4	35.263801094298856	37.0	35.0	37.0	32.0	37.0
5	34.73997285105616	37.0	35.0	37.0	32.0	37.0
6	35.28011306230131	37.0	35.0	37.0	32.0	37.0
7	35.680226070789054	37.0	35.0	37.0	35.0	37.0
8	35.87069784086521	37.0	35.0	37.0	35.0	37.0
9	37.7878058460367	39.0	39.0	39.0	35.0	39.0
10-11	37.87041209083192	39.0	39.0	39.0	35.0	39.0
12-13	37.866347417688786	39.0	39.0	39.0	35.0	39.0
14-15	39.30093084015084	41.0	40.0	41.0	37.0	41.0
16-17	39.239012951579895	41.0	39.5	41.0	36.5	41.0
18-19	39.123493522864706	41.0	39.0	41.0	35.5	41.0
20-21	38.97976502306584	41.0	39.0	41.0	35.0	41.0
22-23	38.893813861567295	40.5	39.0	41.0	35.0	41.0
24-25	38.8268977690241	41.0	39.0	41.0	35.0	41.0
26-27	38.778915037797304	41.0	39.0	41.0	35.0	41.0
28-29	38.626419366425985	41.0	39.0	41.0	35.0	41.0
30-31	38.51810180719407	41.0	38.5	41.0	35.0	41.0
32-33	38.392567001252516	40.0	38.0	41.0	35.0	41.0
34-35	38.31545512150431	40.0	38.0	41.0	35.0	41.0
36-37	38.18301482429198	40.0	38.0	41.0	34.5	41.0
38-39	37.785013460118094	40.0	36.5	41.0	34.0	41.0
40-41	37.59767229422029	40.0	36.0	41.0	33.0	41.0
42-43	37.469394072704816	40.0	35.0	41.0	33.0	41.0
44-45	37.33818112838975	40.0	35.0	41.0	33.0	41.0
46-47	37.2059936204018	40.0	35.0	41.0	33.0	41.0
48-49	37.05952668278383	40.0	35.0	41.0	33.0	41.0
50-51	36.81653287268921	40.0	35.0	41.0	32.5	41.0
52-53	36.3838896983346	39.5	35.0	41.0	31.0	41.0
54-55	35.66333264721037	39.0	35.0	41.0	28.5	41.0
56-57	35.07912813951705	39.0	35.0	41.0	25.0	41.0
58-59	34.7756790935643	39.0	35.0	41.0	23.0	41.0
60-61	34.52973401299867	38.5	35.0	41.0	23.0	41.0
62-63	34.228849587048146	38.0	35.0	40.0	22.0	41.0
64-65	33.953507231870546	37.0	34.5	40.0	21.5	41.0
66-67	33.638554594534966	37.0	34.0	40.0	20.0	41.0
68-69	33.3267534141344	36.0	34.0	39.5	19.5	41.0
70-71	32.97335797994637	36.0	34.0	39.0	18.5	41.0
72-73	32.6161670749771	35.0	34.0	39.0	16.0	40.5
74-75	32.25202722428283	35.0	33.0	37.5	11.5	40.0
76-77	31.877451846948837	35.0	33.0	37.0	7.5	39.0
78-79	31.755206126136308	35.0	33.5	37.0	6.0	39.0
80-81	31.610873434529744	35.0	34.0	36.0	2.0	39.0
82-83	31.308554338920526	35.0	33.5	36.0	2.0	37.5
84-85	31.037115619791017	35.0	33.0	36.0	2.0	37.0
86-87	30.851868070662594	35.0	33.0	35.0	2.0	37.0
88-89	30.652555404429123	35.0	33.0	35.0	2.0	36.0
90-91	30.468549065191098	35.0	33.0	35.0	2.0	36.0
92-93	30.315190493295503	35.0	33.0	35.0	2.0	36.0
94-95	30.1534259733865	35.0	33.0	35.0	2.0	36.0
96-97	29.990805145115004	35.0	33.0	35.0	2.0	35.5
98-99	29.848473510945006	35.0	33.0	35.0	2.0	35.0
100	29.742345760230968	35.0	33.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	2.8046011300550617
1101	2	2.2673944867632336
1101	3	2.036747109521645
1101	4	1.0347570089301357
1101	5	1.6635139108156238
1101	6	1.0391822469827119
1101	7	0.5659003087201526
1101	8	0.4429030250656538
1101	9	0.4459104766424602
1101	10-11	0.38020321800497925
1101	12-13	0.31556722484420163
1101	14-15	0.45876560077383743
1101	16-17	0.40614606591402946
1101	18-19	0.45216497347234963
1101	20-21	0.4810337246568608
1101	22-23	0.428029483642689
1101	24-25	0.5051060222883024
1101	26-27	0.42529107998903726
1101	28-29	0.46125388817726076
1101	30-31	0.40633368143765125
1101	32-33	0.3641064342164242
1101	34-35	0.44432583457314223
1101	36-37	0.4208750809626949
1101	38-39	0.41051797700338
1101	40-41	0.4296534444108815
1101	42-43	0.4258264746876961
1101	44-45	0.434253845446797
1101	46-47	0.47736347577982485
1101	48-49	0.36130833634104675
1101	50-51	0.3826652181787864
1101	52-53	0.301404478900281
1101	54-55	0.3476108878891395
1101	56-57	0.34116576099509643
1101	58-59	0.28858620130743873
1101	60-61	0.32593098683751265
1101	62-63	0.29069676602833994
1101	64-65	0.24391015705432295
1101	66-67	0.25092322668461264
1101	68-69	0.2667197258052667
1101	70-71	0.2521770198567168
1101	72-73	0.28773038699975473
1101	74-75	0.3056007285228972
1101	76-77	0.2261501489657327
1101	78-79	0.3181172089727049
1101	80-81	0.3100181710847032
1101	82-83	0.34752665466641375
1101	84-85	0.40726846429760855
1101	86-87	0.4077306683384698
1101	88-89	0.36258255813176987
1101	90-91	0.29602513776829653
1101	92-93	0.36003858231924823
1101	94-95	0.3851034184677786
1101	96-97	0.4106337705085892
1101	98-99	0.4282512501184641
1101	100	0.45953473186711236
1102	1	2.7561615149096355
1102	2	2.2015681376302716
1102	3	2.074722373746507
1102	4	1.0638380560658476
1102	5	1.6058864544118947
1102	6	1.0175796178450227
1102	7	0.5214494013146478
1102	8	0.4021001726029283
1102	9	0.42697702689883954
1102	10-11	0.36140548081981905
1102	12-13	0.33210612826620434
1102	14-15	0.4591645902421817
1102	16-17	0.42436983569676556
1102	18-19	0.4385881518235806
1102	20-21	0.4310018728664602
1102	22-23	0.38911902018358546
1102	24-25	0.4425806754297099
1102	26-27	0.4082286061459115
1102	28-29	0.48703380472037594
1102	30-31	0.4840682341533764
1102	32-33	0.4490998845336307
1102	34-35	0.45940662597063664
1102	36-37	0.45843898905066993
1102	38-39	0.40195312070791545
1102	40-41	0.321522117640896
1102	42-43	0.3770199015098328
1102	44-45	0.3568183704246124
1102	46-47	0.3378116682284116
1102	48-49	0.38814102368837666
1102	50-51	0.3304751661900198
1102	52-53	0.16692640588580332
1102	54-55	0.22618637085806625
1102	56-57	0.06812232675294894
1102	58-59	0.037047361082173325
1102	60-61	0.0973359096762394
1102	62-63	0.07773819440004814
1102	64-65	0.0975234794626445
1102	66-67	0.09724775334596103
1102	68-69	0.05427109078713244
1102	70-71	0.09627331665060979
1102	72-73	0.15873382778869427
1102	74-75	0.1559166736465798
1102	76-77	0.08019125224323176
1102	78-79	0.17219616612538502
1102	80-81	0.22342857041678243
1102	82-83	0.28985319618047
1102	84-85	0.25130814067648544
1102	86-87	0.21040217029234043
1102	88-89	0.24247383217836216
1102	90-91	0.22371602379342193
1102	92-93	0.2190435316171886
1102	94-95	0.22174473137875594
1102	96-97	0.19935347289243666
1102	98-99	0.16421477656238181
1102	100	0.1604723081964039
1103	1	2.5339219858021345
1103	2	2.1433991488652744
1103	3	1.9421378867530343
1103	4	0.9518776570184002
1103	5	1.5075206518583357
1103	6	0.9467097309046366
1103	7	0.41753210006969255
1103	8	0.3601158062906933
1103	9	0.32360542255493385
1103	10-11	0.21667007943563021
1103	12-13	0.16606885925362036
1103	14-15	0.26787640759338416
1103	16-17	0.34842772365303176
1103	18-19	0.2944844032540743
1103	20-21	0.33206568096223066
1103	22-23	0.3116769041803309
1103	24-25	0.37725045538195445
1103	26-27	0.33079564069058165
1103	28-29	0.42374233298740194
1103	30-31	0.4325072972096038
1103	32-33	0.3810131827334331
1103	34-35	0.3377933185859874
1103	36-37	0.2802127537095558
1103	38-39	0.33818268716025557
1103	40-41	0.3544002078370525
1103	42-43	0.38343993841007773
1103	44-45	0.3193088714699499
1103	46-47	0.3518486087565833
1103	48-49	0.2828924502229455
1103	50-51	0.3067016217678358
1103	52-53	0.3537629171145369
1103	54-55	0.3069414996388602
1103	56-57	0.17814309023476227
1103	58-59	0.13046815764937492
1103	60-61	0.10580789849280592
1103	62-63	0.06544286659188714
1103	64-65	0.07690833941187236
1103	66-67	0.08726751416467238
1103	68-69	0.12361374036554196
1103	70-71	0.13380096081445458
1103	72-73	0.08224012632751965
1103	74-75	0.08592765555119541
1103	76-77	0.1064356160824893
1103	78-79	0.05069955550704819
1103	80-81	0.0847407614844613
1103	82-83	0.13351865688701992
1103	84-85	0.11610349482950255
1103	86-87	0.17373718740963184
1103	88-89	0.13552651482906697
1103	90-91	0.17190325192315825
1103	92-93	0.13129549798732043
1103	94-95	0.13542376233229803
1103	96-97	0.09468325458686166
1103	98-99	0.11180357132828078
1103	100	0.17583057537506974
1104	1	1.4783985152195136
1104	2	1.6879636190969798
1104	3	1.75818528698019
1104	4	0.8916072083288071
1104	5	1.5334400786901412
1104	6	1.055026696321839
1104	7	0.5082886875471857
1104	8	0.3851964947815887
1104	9	0.33355062427089877
1104	10-11	0.28571224421959585
1104	12-13	0.24872345419476005
1104	14-15	0.371030185886724
1104	16-17	0.328675160928789
1104	18-19	0.3529160089665737
1104	20-21	0.4308608289082585
1104	22-23	0.34395791859144964
1104	24-25	0.4052553698329291
1104	26-27	0.3351515994104801
1104	28-29	0.39920123690019693
1104	30-31	0.3503578993776202
1104	32-33	0.38014517862260533
1104	34-35	0.29216056378670174
1104	36-37	0.3629608250769891
1104	38-39	0.3573679794208786
1104	40-41	0.3098727739944067
1104	42-43	0.3106175582453119
1104	44-45	0.3518539342673961
1104	46-47	0.39574074486864674
1104	48-49	0.3843014115574164
1104	50-51	0.2890337986071003
1104	52-53	0.2846028801294409
1104	54-55	0.18732999273505158
1104	56-57	0.1532278136463745
1104	58-59	0.10776106507705663
1104	60-61	0.17810019724743142
1104	62-63	0.22010766696650563
1104	64-65	0.20559731871677656
1104	66-67	0.17324549146670876
1104	68-69	0.1232556506945528
1104	70-71	0.0755717575941901
1104	72-73	0.0824088510480081
1104	74-75	0.17909583712219757
1104	76-77	0.15341395510761302
1104	78-79	0.1761994360776704
1104	80-81	0.2667691636012073
1104	82-83	0.3351481786202193
1104	84-85	0.24069760211100544
1104	86-87	0.15663518440049629
1104	88-89	0.180030255153234
1104	90-91	0.17809967186305187
1104	92-93	0.24322337394002602
1104	94-95	0.19137243386633784
1104	96-97	0.3263419446900855
1104	98-99	0.3187413571866884
1104	100	0.31672338073742523
1105	1	2.131287083807617
1105	2	1.9688778068203412
1105	3	1.85860644309356
1105	4	0.9129046482631722
1105	5	1.5216647672284225
1105	6	0.9997861344157073
1105	7	0.5133927643602405
1105	8	0.3715712540940288
1105	9	0.34519075894505846
1105	10-11	0.2561631661315005
1105	12-13	0.20344734629281191
1105	14-15	0.3020548108576122
1105	16-17	0.25668283336456454
1105	18-19	0.2403984965210384
1105	20-21	0.33556265720978473
1105	22-23	0.33912765460200944
1105	24-25	0.3769643938148306
1105	26-27	0.24878655260344829
1105	28-29	0.27193844119173605
1105	30-31	0.31053047744169504
1105	32-33	0.28823105372747193
1105	34-35	0.28257268002631264
1105	36-37	0.31568159054659617
1105	38-39	0.24064739134862378
1105	40-41	0.1920395940358759
1105	42-43	0.27800476863488655
1105	44-45	0.29461129533385133
1105	46-47	0.3184988894400931
1105	48-49	0.27473454938407116
1105	50-51	0.32426172095140515
1105	52-53	0.31577366574103394
1105	54-55	0.2082275051066702
1105	56-57	0.17008720036891845
1105	58-59	0.14419832000075417
1105	60-61	0.23444804390219787
1105	62-63	0.20991439993543537
1105	64-65	0.201848055100605
1105	66-67	0.22614458563145234
1105	68-69	0.14400061612054316
1105	70-71	0.13826667479028742
1105	72-73	0.19064230597244602
1105	74-75	0.1961346021409156
1105	76-77	0.09670720402936794
1105	78-79	0.05270837431751829
1105	80-81	0.09701955258187667
1105	82-83	0.17889361341006094
1105	84-85	0.2300499760942074
1105	86-87	0.15695155780715098
1105	88-89	0.15292750142881317
1105	90-91	0.15451686610207105
1105	92-93	0.16388271557407563
1105	94-95	0.2205800811708194
1105	96-97	0.2402591962136711
1105	98-99	0.20910368390088507
1105	100	0.19519051972456225
1106	1	1.65509962347231
1106	2	1.092421754395854
1106	3	1.5246515203195905
1106	4	0.7839546248723295
1106	5	1.4944085015665323
1106	6	1.0120988390855317
1106	7	0.5712644665241697
1106	8	0.36644566524329747
1106	9	0.30770719236820554
1106	10-11	0.28062621042177227
1106	12-13	0.1993656778189461
1106	14-15	0.28821805351670093
1106	16-17	0.2565119243132159
1106	18-19	0.23893831352853567
1106	20-21	0.3266288224542606
1106	22-23	0.25968079532056265
1106	24-25	0.34621927415891207
1106	26-27	0.2649306111494525
1106	28-29	0.32924722562975717
1106	30-31	0.2971784217678817
1106	32-33	0.28787889288554425
1106	34-35	0.29326965444871433
1106	36-37	0.23249509848316308
1106	38-39	0.2947920663973136
1106	40-41	0.2784261412219067
1106	42-43	0.3241866804459903
1106	44-45	0.283030241549433
1106	46-47	0.2515450437325839
1106	48-49	0.24246299808198302
1106	50-51	0.1707191296818067
1106	52-53	0.2471860734016218
1106	54-55	0.34158839490137183
1106	56-57	0.388585269264901
1106	58-59	0.41024085868630067
1106	60-61	0.4008886692535114
1106	62-63	0.4019890910494581
1106	64-65	0.33729925165979324
1106	66-67	0.33503816638786077
1106	68-69	0.41026953459350324
1106	70-71	0.38002324326378556
1106	72-73	0.3462414196591723
1106	74-75	0.3948708006402768
1106	76-77	0.21025625044476115
1106	78-79	0.24460690522787587
1106	80-81	0.26159704878313406
1106	82-83	0.332345208882753
1106	84-85	0.32619010352893696
1106	86-87	0.3130753370411661
1106	88-89	0.3046078919288675
1106	90-91	0.3100306558166288
1106	92-93	0.3614703623107829
1106	94-95	0.3525734664596136
1106	96-97	0.3576503889287821
1106	98-99	0.43637236665222545
1106	100	0.3996280335505418
1107	1	1.6456853464753642
1107	2	1.1122288974828933
1107	3	1.3689178248379825
1107	4	0.7353273967256513
1107	5	1.4539352558352263
1107	6	0.972016209297017
1107	7	0.5551281789622777
1107	8	0.4031243847624495
1107	9	0.39517306362934335
1107	10-11	0.2178712327769361
1107	12-13	0.16006822075480898
1107	14-15	0.2653341614330884
1107	16-17	0.25862070298593665
1107	18-19	0.25000031971813996
1107	20-21	0.2002395155578185
1107	22-23	0.2516100004298707
1107	24-25	0.29867862841908277
1107	26-27	0.23088597695114998
1107	28-29	0.24325578696593197
1107	30-31	0.18147861864645876
1107	32-33	0.15641587782973687
1107	34-35	0.07745542083251422
1107	36-37	0.20402804092923077
1107	38-39	0.19112640991333052
1107	40-41	0.21222307292206466
1107	42-43	0.19022210689591645
1107	44-45	0.1832970836268899
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2214	4	-1.4202353525053155
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2214	96-97	-0.42823882493367904
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2214	100	-0.4238773061922956
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2215	3	-3.5266206721958966
2215	4	-1.7267698802980505
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2215	6	-4.883316693300678
2215	7	-1.960954148609133
2215	8	-0.9917583773682139
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2215	24-25	-0.7975987670699567
2215	26-27	-0.7717301699578059
2215	28-29	-0.8218138266624635
2215	30-31	-0.9267123249846705
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2215	100	-0.7858507689664052
2216	1	-6.007305806168279
2216	2	-4.937003593164718
2216	3	-6.022595710124666
2216	4	-3.123297099948708
2216	5	-4.210903264008184
2216	6	-2.0277797459619293
2216	7	-1.287950787191562
2216	8	-0.8659386567652518
2216	9	-0.7121245129400293
2216	10-11	-1.0137626351322808
2216	12-13	-0.7696509051020968
2216	14-15	-1.634639849743131
2216	16-17	-1.1666507385161964
2216	18-19	-1.745256618945092
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2216	24-25	-1.584712521216403
2216	26-27	-1.3258582253704887
2216	28-29	-2.06064758333671
2216	30-31	-2.1222024031757982
2216	32-33	-1.2579407082860712
2216	34-35	-1.3312638310995553
2216	36-37	-1.3701802904916391
2216	38-39	-1.2638481004981585
2216	40-41	-1.3142193976949201
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2216	52-53	-1.2986039810541072
2216	54-55	-1.330518799701899
2216	56-57	-1.1884724240368385
2216	58-59	-1.2277197507534652
2216	60-61	-1.1944163733408502
2216	62-63	-1.2120356387957472
2216	64-65	-1.3493170026816088
2216	66-67	-1.2752486833989352
2216	68-69	-1.2097075355169764
2216	70-71	-1.1107860586093707
2216	72-73	-1.1830039701317858
2216	74-75	-1.0587820412608195
2216	76-77	-1.0286576437786863
2216	78-79	-1.0341732576614682
2216	80-81	-1.0788411252336054
2216	82-83	-1.0604466902267369
2216	84-85	-1.0262539595451727
2216	86-87	-1.12899626321936
2216	88-89	-1.2315276156101582
2216	90-91	-1.2114662337300288
2216	92-93	-1.2026281036004178
2216	94-95	-1.1960709802997087
2216	96-97	-1.257970446319078
2216	98-99	-1.255995007889286
2216	100	-1.320014834223464
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	5998.0
3	4709.0
4	5173.0
5	6708.0
6	5636.0
7	4150.0
8	3803.0
9	4098.0
10	4459.0
11	8039.0
12	5647.0
13	7600.0
14	7143.0
15	7557.0
16	8809.0
17	11306.0
18	15887.0
19	26420.0
20	53888.0
21	72684.0
22	35170.0
23	23579.0
24	23778.0
25	25221.0
26	25374.0
27	28151.0
28	32306.0
29	37263.0
30	44161.0
31	55208.0
32	71745.0
33	98675.0
34	144400.0
35	310192.0
36	418958.0
37	745326.0
38	988190.0
39	336687.0
40	2437.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	15.345227848684019	41.6167681597944	26.313808548576034	16.724195442945554
2	18.219671799223924	39.717528058183106	24.412961744373014	17.64983839821996
3	19.329160091321622	25.656478413360833	21.487164791936575	33.52719670338097
4	31.976612624393418	26.725377266728284	22.618675728871114	18.679334380007184
5	17.910042553076995	38.527714658949805	23.857383288466274	19.70485949950693
6	34.46261100729578	24.490257726619014	23.820009767162155	17.227121498923058
7	19.003830180531057	24.311677409199696	38.045276043411405	18.639216366857838
8	33.58574586274581	24.762608182083582	23.434570103604567	18.21707585156604
9	33.350015538667066	26.132647748507683	23.23365715646429	17.283679556360966
10-11	17.46052438628992	41.44849974505823	23.392878043661636	17.698097824990214
12-13	17.35396007302501	25.83652515044255	31.037485184452724	25.772029592079722
14-15	17.585291138116553	33.40242995155434	23.383971898556048	25.62830701177306
16-17	25.615385526076462	25.585048103985113	30.933219463736624	17.8663469062018
18-19	17.892580051042167	25.734104481728277	30.959886022868076	25.41342944436148
20-21	25.732074989405422	33.595320897881756	23.14585533529305	17.52674877741977
22-23	25.433293726517086	25.917912182647697	30.952740350412693	17.696053740422524
24-25	17.764571031888572	33.490805279648924	23.182776430196405	25.561847258266102
26-27	18.088695370933184	29.042425152383284	35.19837877588054	17.670500700802997
28-29	24.931673009321802	25.77227493529873	31.647905080675965	17.648146974703508
30-31	23.032771589372103	26.83843814575923	31.997152554540946	18.131637710327723
32-33	19.85407444240309	27.916816001360427	32.886244095225486	19.342865461010998
34-35	19.796001560629094	27.753999112055862	33.12077385677192	19.329225470543125
36-37	19.73135993901357	27.77960982838417	33.36088809202536	19.128142140576898
38-39	20.40278400446061	27.601433336042273	32.91818327132365	19.07759938817347
40-41	19.50679915628413	28.497461398976487	32.90389641420756	19.091843030531827
42-43	19.655297679112007	27.764776320215272	33.24874537504204	19.331180625630676
44-45	19.45783978650686	28.198051589870595	32.83328839817181	19.510820225450736
46-47	19.94193920056484	27.84983730437307	33.05968950468579	19.148533990376297
48-49	19.509814776969666	28.005106538928747	32.936664844568476	19.548413839533108
50-51	19.961831819706475	28.502271617569043	32.34770770934543	19.188188853379053
52-53	20.123315225807268	28.258449920965408	32.167477193383235	19.45075765984409
54-55	20.110981672256425	29.154134051147196	30.81822681445388	19.9166574621425
56-57	20.406192469609962	29.661142134806973	29.967612122542793	19.965053273040272
58-59	21.28667042737393	29.86801155226847	28.979393109026386	19.865924911331216
60-61	20.92542375845852	30.368559029517954	28.749622440076443	19.95639477194708
62-63	20.49129887864172	30.406366247586757	28.662469140785284	20.43986573298623
64-65	20.71200210750708	30.315286503370746	29.002709459752186	19.97000192936999
66-67	20.576664510749694	29.851045287845864	29.505981963222755	20.066308238181687
68-69	20.899561489136662	29.810679181222138	29.40789125802471	19.88186807161649
70-71	20.063764626293707	30.452425846308845	29.483981747860096	19.99982777953736
72-73	20.0261814972426	29.780255706853335	29.71548811809389	20.47807467781017
74-75	20.18661032632927	30.153309828893672	29.23404819800342	20.426031646773637
76-77	20.57947039023948	29.84886551595258	29.372592906121287	20.199071187686656
78-79	20.410504806728426	30.132588361966313	28.881033236031723	20.575873595273546
80-81	20.822329739169746	30.587063163814747	28.46413415818805	20.12647293882746
82-83	20.81774208391478	30.480755828252555	28.51146868358639	20.190033404246275
84-85	20.573688697805522	30.84935273245669	28.041694634269852	20.535263935467928
86-87	20.631292864807047	30.95394902808938	28.22972004803213	20.18503805907145
88-89	21.45836901429333	30.541121350602985	27.87377086520515	20.126738769898537
90-91	21.02918306339086	30.991440045539715	27.77396650688928	20.20541038418014
92-93	20.65943324649121	31.036181318174915	27.664488639459783	20.639896795874098
94-95	20.89862838678567	31.19227987238605	27.520216844697604	20.38887489613068
96-97	20.862222119367008	30.81478284199187	27.76274118240024	20.56025385624088
98-99	21.31468357604782	30.955083873321716	27.203198331305405	20.527034219325063
100	20.701603042918585	31.630122655277965	27.19094550902586	20.477328792777588
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	108472.0
1	58674.5
2	6454.0
3	3411.5
4	2637.0
5	2401.5
6	2382.5
7	2670.5
8	2592.5
9	2375.5
10	2406.0
11	3661.5
12	31486.5
13	35325.5
14	11354.0
15	10581.5
16	11744.0
17	13190.5
18	14523.5
19	15710.0
20	17145.5
21	19041.5
22	21500.0
23	24610.0
24	27970.5
25	31550.5
26	36232.0
27	41545.0
28	46328.0
29	50822.5
30	55977.0
31	60825.0
32	65709.0
33	70937.0
34	76834.0
35	82186.0
36	86849.5
37	92369.5
38	97486.0
39	101488.0
40	105000.5
41	108348.0
42	110966.5
43	114113.5
44	116589.5
45	117650.0
46	118884.5
47	121425.5
48	122222.0
49	124901.5
50	143510.0
51	139596.0
52	111557.0
53	99862.0
54	94688.5
55	90184.5
56	83437.0
57	74362.5
58	66544.5
59	56448.0
60	45166.0
61	36534.5
62	28978.0
63	22166.0
64	16816.5
65	12850.5
66	9843.0
67	7664.0
68	5952.0
69	4280.5
70	3017.5
71	2192.0
72	1615.0
73	1166.5
74	903.5
75	667.0
76	447.0
77	304.0
78	176.5
79	111.5
80	65.0
81	36.0
82	21.5
83	17.0
84	10.0
85	5.0
86	5.0
87	2.0
88	1.5
89	1.0
90	1.0
91	0.0
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.058172464405689704
2	0.06637903315857377
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	8.072034838902366E-5
18-19	0.0
20-21	2.6906782796341214E-4
22-23	8.072034838902366E-5
24-25	0.0
26-27	0.002260169754892662
28-29	8.475636580847482E-4
30-31	0.0059598523893895795
32-33	0.001372245922613402
34-35	0.0022870765376890034
36-37	0.008031674664707852
38-39	0.012081145475557206
40-41	0.0031750003699682634
42-43	0.007668433096957247
44-45	0.001856568012947544
46-47	0.002313983320485345
48-49	0.00332298767534814
50-51	0.009430827370117596
52-53	0.003349894458144481
54-55	0.0017623942731603496
56-57	5.650424387231655E-4
58-59	9.551907892701132E-4
60-61	0.006390360914131039
62-63	0.0016144069677804728
64-65	0.007964407707717001
66-67	0.010910700423916363
68-69	0.011717903907806599
70-71	0.009901696069053566
72-73	0.004574153075378007
74-75	0.0019911019269292503
76-77	0.004735593772156054
78-79	0.016090256112212046
80-81	0.008744704408810896
82-83	0.006484534653918232
84-85	0.0048835810775359305
86-87	0.007332098312002981
88-89	0.004049470810849353
90-91	0.005879132041000556
92-93	0.010883793641120023
94-95	0.008798517974403577
96-97	0.011193221643277945
98-99	0.002609957931245098
100	0.002771398628023145
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3716535.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.70762298350621
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.05593549170982	55.88823341798693
2	11.11234742810833	14.603318705357541
3	2.134638250018275	4.207860160151168
4	0.5605429433832182	1.4732777755955746
5	0.2707511263316988	0.8895206465681463
6	0.16031606366685516	0.6320392481772897
7	0.11854528001824763	0.5452529996139232
8	0.0898286600059039	0.4721942179825196
9	0.06131904370312156	0.3626215744818467
>10	0.3957186673009019	5.797668240231153
>50	0.028783473462862766	1.1986748995235834
>100	0.007522778629818571	1.0589862871943545
>500	0.0021433106666535716	1.0386890531282378
>1k	0.0014426129436223476	1.6631970503874982
>5k	4.121751267492422E-5	0.25087027206427837
>10k+	1.2365253802477266E-4	9.917595451555892
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	236559	6.365041631519682	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	109342	2.942041444517541	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	20291	0.5459655297205596	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	9263	0.2492375290425087	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4865	0.1309014983042	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4630	0.12457840434705982	No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4361	0.11734047977484405	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0014529662710024256	2.6906782796341216E-5	0.0	0.0	0.0
2	0.0014529662710024256	2.6906782796341216E-5	0.0	2.6906782796341216E-5	0.0
3	0.0014529662710024256	2.6906782796341216E-5	0.0	2.6906782796341216E-5	0.0
4	0.0014529662710024256	2.6906782796341216E-5	0.0	2.6906782796341216E-5	0.0
5	0.0014529662710024256	8.072034838902364E-5	0.0	2.6906782796341216E-5	0.0
6	0.0014529662710024256	1.3453391398170607E-4	0.0	2.6906782796341216E-5	0.0
7	0.0014529662710024256	1.614406967780473E-4	0.0	2.6906782796341216E-5	0.0
8	0.0014529662710024256	1.614406967780473E-4	0.0	2.6906782796341216E-5	0.0
9	0.0014529662710024256	1.883474795743885E-4	0.0	2.6906782796341216E-5	0.0
10-11	0.001479873053798767	2.4216104516707094E-4	0.0	2.6906782796341216E-5	0.0
12-13	0.0015202332279932785	2.959746107597534E-4	0.0	2.6906782796341216E-5	0.0
14-15	0.0015471400107896199	3.497881763524358E-4	0.0	2.6906782796341216E-5	0.0
16-17	0.0015605934021877905	4.439619161396301E-4	0.0	2.6906782796341216E-5	0.0
18-19	0.0016009535763823024	4.7086869893597127E-4	0.0	2.6906782796341216E-5	0.0
20-21	0.001681673924771326	5.246822645286537E-4	0.0	5.381356559268243E-5	0.0
22-23	0.0017758476645585203	5.650424387231655E-4	0.0	5.381356559268243E-5	0.0
24-25	0.001856568012947544	5.919492215195068E-4	0.0	5.381356559268243E-5	0.0
26-27	0.0018969281871420557	5.919492215195068E-4	0.0	5.381356559268243E-5	0.0
28-29	0.0019103815785402264	6.18856004315848E-4	0.0	5.381356559268243E-5	0.0
30-31	0.0019776485355310794	6.323093957140186E-4	0.0	5.381356559268243E-5	0.0
32-33	0.002152542623707297	6.592161785103597E-4	0.0	5.381356559268243E-5	0.0
34-35	0.0023139833204853444	7.668433096957247E-4	0.0	5.381356559268243E-5	0.0
36-37	0.0025292375828560743	8.072034838902365E-4	0.0	5.381356559268243E-5	0.0
38-39	0.002798305410819486	8.341102666865777E-4	0.0	5.381356559268243E-5	0.0
40-41	0.003296080892551799	8.879238322792601E-4	0.0	6.726695699085303E-5	0.0
42-43	0.003968750462460329	9.686441806682838E-4	0.0	8.072034838902364E-5	0.0
44-45	0.004614513249572518	0.0010224577462609663	0.0	8.072034838902364E-5	0.0
46-47	0.005394809950666414	0.001062817920455478	0.0	8.072034838902364E-5	0.0
48-49	0.006403814305529209	0.00110317809464999	0.0	8.072034838902364E-5	0.0
50-51	0.007601166139966393	0.0011569916602426722	0.0	8.072034838902364E-5	0.0
52-53	0.009323200238932232	0.001237712008631696	0.0	8.072034838902364E-5	0.0
54-55	0.011569916602426723	0.001237712008631696	0.0	8.072034838902364E-5	0.0
56-57	0.014314408447653526	0.0012915255742243783	0.0	8.072034838902364E-5	0.0
58-59	0.01767775629719618	0.0013318857484188903	0.0	8.072034838902364E-5	0.0
60-61	0.02215773563278699	0.0013453391398170608	0.0	8.072034838902364E-5	0.0
62-63	0.028279028718954616	0.001372245922613402	0.0	9.417373978719425E-5	0.0
64-65	0.036028182164300884	0.0014664196624005962	0.0	1.3453391398170607E-4	0.0
66-67	0.04685816223982823	0.001506779836595108	0.0	1.3453391398170607E-4	0.0
68-69	0.06098422320790736	0.001506779836595108	0.0	1.3453391398170607E-4	0.0
70-71	0.08085488230300536	0.001506779836595108	0.0	1.614406967780473E-4	0.0
72-73	0.10469429186056367	0.001506779836595108	0.0	1.883474795743885E-4	1.3453391398170608E-5
74-75	0.13641738877744997	0.0015471400107896199	0.0	1.883474795743885E-4	2.6906782796341216E-5
76-77	0.17821707585156604	0.001614406967780473	0.0	2.4216104516707094E-4	2.6906782796341216E-5
78-79	0.22908434872804911	0.0016278603591786435	0.0	0.0014529662710024256	2.6906782796341216E-5
80-81	0.29570554293178997	0.001681673924771326	0.0	0.0014529662710024256	2.6906782796341216E-5
82-83	0.38016593412950506	0.0017354874903640085	0.0	0.0014529662710024256	2.6906782796341216E-5
84-85	0.48533109468900465	0.0018027544473548614	0.0	0.0014529662710024256	2.6906782796341216E-5
86-87	0.6137437155845431	0.0018027544473548614	0.0	0.0014529662710024256	2.6906782796341216E-5
88	0.7291200002152542	0.0018027544473548614	0.0	0.0014529662710024256	2.6906782796341216E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	2495	0.0	80.53166	1
AACGAGT	67695	0.0	72.173134	1
ACGAGTG	71560	0.0	71.38214	2
GTGGAAT	75345	0.0	71.13783	6
GAATCAC	76565	0.0	70.61197	9
CGAGTGG	73405	0.0	70.508224	3
TGGAATC	76670	0.0	70.33138	7
GGAATCA	77035	0.0	70.16286	8
AGTGGAA	74795	0.0	69.93931	5
GAGTGGA	75930	0.0	68.98052	4
AACGTGT	1690	0.0	57.914318	1
CGTGTGG	2085	0.0	57.251575	3
ACGTGTG	2065	0.0	53.7356	2
ATCGAGT	1115	0.0	53.596718	1
TCGAGTG	1240	0.0	53.464756	2
GAGTACT	35695	0.0	41.519115	20-21
AGTACTT	36510	0.0	41.351757	20-21
GTACTTT	36230	0.0	40.231762	22-23
TACTTTT	38405	0.0	38.791546	22-23
CGATTGG	735	0.0	37.724583	3
>>END_MODULE