FastQCFastQC Report
Thu 26 May 2016
SRR2132683_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132683_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4712954
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3293416.987995214890703No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1631783.4623295707957262No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG329120.6983306011473908No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT130290.276450820440853No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA105170.2231509155404445No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA71720.1521763208382683No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT66750.14163091767923047No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT60420.12819985087908772No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT34300.084.645651
AACGAGT994600.069.467231
GTGGAAT1095000.069.129726
ACGAGTG1050800.068.821142
TGGAATC1110350.068.47037
GAATCAC1115150.068.23889
GGAATCA1115600.068.19028
CGAGTGG1078300.068.008233
AGTGGAA1090950.067.792565
GAGTGGA1114950.066.4765854
AACGTGT21900.062.5262641
TCGAGTG16850.062.511792
CGTGTGG26250.062.2998853
ATCGAGT15200.061.9155961
ACGTGTG27450.056.0169222
GACGAGT10050.045.4170881
GAGTACT504600.041.16481820-21
AGTACTT516800.041.0614720-21
GTACTTT511300.039.97281622-23
ACGATTG8000.039.9698752
Produced by FastQC (version 0.11.5)