##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132683_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4712954
Sequences flagged as poor quality	0
Sequence length	100
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.168406269189134	31.0	28.0	34.0	16.0	34.0
2	30.289057987835232	33.0	30.0	34.0	19.0	34.0
3	30.739630813286105	33.0	31.0	34.0	25.0	34.0
4	35.16975234640525	37.0	35.0	37.0	32.0	37.0
5	34.63225505701944	37.0	35.0	37.0	30.0	37.0
6	35.16784908148902	37.0	35.0	37.0	32.0	37.0
7	35.56939448167752	37.0	35.0	37.0	33.0	37.0
8	35.77008771993107	37.0	35.0	37.0	35.0	37.0
9	37.6766770904193	39.0	38.0	39.0	35.0	39.0
10-11	37.766705764579925	39.0	39.0	39.0	35.0	39.0
12-13	37.75812293096856	39.0	39.0	39.0	35.0	39.0
14-15	39.17513474563936	41.0	39.0	41.0	36.0	41.0
16-17	39.10405989534377	41.0	39.0	41.0	36.0	41.0
18-19	38.960989646832964	41.0	39.0	41.0	35.5	41.0
20-21	38.80582761894132	41.0	39.0	41.0	35.0	41.0
22-23	38.7092497614023	40.5	39.0	41.0	35.0	41.0
24-25	38.63071239396778	41.0	39.0	41.0	35.0	41.0
26-27	38.57840199586077	40.5	39.0	41.0	35.0	41.0
28-29	38.407602005026995	41.0	38.5	41.0	35.0	41.0
30-31	38.28280670679153	40.5	38.0	41.0	34.5	41.0
32-33	38.14779711408174	40.0	38.0	41.0	34.0	41.0
34-35	38.068499607677055	40.0	38.0	41.0	34.0	41.0
36-37	37.916473192821314	40.0	38.0	41.0	34.0	41.0
38-39	37.48881136968449	40.0	36.0	41.0	33.5	41.0
40-41	37.29594082607214	40.0	35.0	41.0	33.0	41.0
42-43	37.16398123130419	40.0	35.0	41.0	33.0	41.0
44-45	37.02731683780491	40.0	35.0	41.0	33.0	41.0
46-47	36.890027782999795	40.0	35.0	41.0	33.0	41.0
48-49	36.73801081020523	40.0	35.0	41.0	32.5	41.0
50-51	36.48334144572597	40.0	35.0	41.0	31.5	41.0
52-53	36.022946860928414	39.0	35.0	41.0	30.5	41.0
54-55	35.24317063141291	39.0	35.0	41.0	25.5	41.0
56-57	34.621665095818884	39.0	35.0	41.0	21.0	41.0
58-59	34.31100918871689	39.0	35.0	41.0	18.0	41.0
60-61	34.06582421555568	38.5	35.0	41.0	14.5	41.0
62-63	33.77392172722246	38.0	34.5	40.0	11.0	41.0
64-65	33.50777622696933	37.5	34.0	40.0	9.0	41.0
66-67	33.19487682247694	37.0	34.0	40.0	7.0	41.0
68-69	32.8986010684594	36.0	34.0	39.5	4.5	41.0
70-71	32.56014306526225	36.0	34.0	39.0	2.0	41.0
72-73	32.216709732367434	35.0	33.0	39.0	2.0	41.0
74-75	31.864845593655275	35.0	33.0	38.5	2.0	40.0
76-77	31.504718484415505	35.0	33.0	37.0	2.0	39.5
78-79	31.36551544954608	35.0	33.0	37.0	2.0	39.0
80-81	31.210557646011395	35.0	33.0	37.0	2.0	39.0
82-83	30.882853726134393	35.0	33.0	36.0	2.0	39.0
84-85	30.582996248212904	35.0	33.0	36.0	2.0	37.0
86-87	30.37101486668446	35.0	33.0	35.5	2.0	37.0
88-89	30.145528791496798	35.0	33.0	35.0	2.0	36.5
90-91	29.930895676045218	35.0	33.0	35.0	2.0	36.0
92-93	29.757102127455518	35.0	33.0	35.0	2.0	36.0
94-95	29.572336903776275	35.0	33.0	35.0	2.0	36.0
96-97	29.384492295065897	35.0	32.5	35.0	2.0	36.0
98-99	29.23304884792001	35.0	32.0	35.0	2.0	35.0
100	29.1241788059039	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	2.9199479804040287
1101	2	2.3352945006852117
1101	3	2.11484742697775
1101	4	1.1166742443399826
1101	5	1.7175029349680457
1101	6	1.0829518137876875
1101	7	0.5982772298235659
1101	8	0.45518422549879034
1101	9	0.4352101196317619
1101	10-11	0.4290886908149716
1101	12-13	0.3742560382243667
1101	14-15	0.519506722891272
1101	16-17	0.498095313726985
1101	18-19	0.5313775480511183
1101	20-21	0.5527698992248702
1101	22-23	0.49839438444621464
1101	24-25	0.5674823956443973
1101	26-27	0.5455796882678214
1101	28-29	0.5322067870310008
1101	30-31	0.5172665148756579
1101	32-33	0.5503030460034921
1101	34-35	0.5851592372506076
1101	36-37	0.5383498584384938
1101	38-39	0.49820471548650147
1101	40-41	0.5129995198465807
1101	42-43	0.45258750834307904
1101	44-45	0.43668235437406366
1101	46-47	0.423328350529772
1101	48-49	0.396666705638296
1101	50-51	0.4664542891922565
1101	52-53	0.4240409102618017
1101	54-55	0.36797124525471503
1101	56-57	0.2536782662648065
1101	58-59	0.2212358968684569
1101	60-61	0.25870924833446196
1101	62-63	0.2500380251135894
1101	64-65	0.17385448002544024
1101	66-67	0.22764241738772029
1101	68-69	0.21236142402312197
1101	70-71	0.1827414922207069
1101	72-73	0.13992103551572654
1101	74-75	0.1666059157730828
1101	76-77	0.10115589872324193
1101	78-79	0.16319500046739321
1101	80-81	0.1845469715864141
1101	82-83	0.2745641104596146
1101	84-85	0.2632639820356033
1101	86-87	0.26149890334345827
1101	88-89	0.23519217224501432
1101	90-91	0.14385635324413215
1101	92-93	0.20743649502438544
1101	94-95	0.26784515184175106
1101	96-97	0.2593765688494152
1101	98-99	0.22749304915100765
1101	100	0.2304388105170574
1102	1	2.8076588963694995
1102	2	2.2801903758689086
1102	3	2.0886125053110867
1102	4	1.0958796177888246
1102	5	1.5863977053679648
1102	6	1.076237640714922
1102	7	0.5451402427018905
1102	8	0.48758435771407704
1102	9	0.44968917107939177
1102	10-11	0.4161045796000664
1102	12-13	0.40416196541045935
1102	14-15	0.5304171222357965
1102	16-17	0.5121844725217102
1102	18-19	0.529219420992014
1102	20-21	0.5493053152216021
1102	22-23	0.48112738949239997
1102	24-25	0.5289369695890755
1102	26-27	0.48163360002810407
1102	28-29	0.6119765023961676
1102	30-31	0.5487002250788393
1102	32-33	0.5439918235581302
1102	34-35	0.5508693918965974
1102	36-37	0.5440263841836739
1102	38-39	0.5159205618265972
1102	40-41	0.5374246590063407
1102	42-43	0.6248713902916023
1102	44-45	0.5797928746960963
1102	46-47	0.5465969488445879
1102	48-49	0.6032248688904218
1102	50-51	0.595918434764144
1102	52-53	0.4905892285925333
1102	54-55	0.4686214532458237
1102	56-57	0.43382489544760716
1102	58-59	0.40255761185798633
1102	60-61	0.4048361607671609
1102	62-63	0.41909988482768057
1102	64-65	0.4572155604554382
1102	66-67	0.3663054443528253
1102	68-69	0.405013899617245
1102	70-71	0.3951723146330366
1102	72-73	0.3846843260936481
1102	74-75	0.4596444061973699
1102	76-77	0.3765021526805299
1102	78-79	0.40775226522534425
1102	80-81	0.43107282492504595
1102	82-83	0.5137789489614875
1102	84-85	0.48521367216591216
1102	86-87	0.47171303565951916
1102	88-89	0.5024439328952397
1102	90-91	0.48580408072130155
1102	92-93	0.5162938642197545
1102	94-95	0.5297361523121005
1102	96-97	0.5220622591823201
1102	98-99	0.5586922170282875
1102	100	0.6013367269655419
1103	1	2.623847155875712
1103	2	2.2520576383911326
1103	3	2.0475805408911434
1103	4	1.0788771088458375
1103	5	1.6384658830990375
1103	6	1.04550381538251
1103	7	0.5864953409276055
1103	8	0.46739088254172145
1103	9	0.42822144639624327
1103	10-11	0.3222559369868989
1103	12-13	0.29832138753260296
1103	14-15	0.410472430247772
1103	16-17	0.469329239530893
1103	18-19	0.45231902710703054
1103	20-21	0.5372720828994204
1103	22-23	0.47491976822240645
1103	24-25	0.5571430588387116
1103	26-27	0.5229157742760933
1103	28-29	0.5718031009166253
1103	30-31	0.584429890063511
1103	32-33	0.5443595971849078
1103	34-35	0.5762849501287448
1103	36-37	0.4804519369205664
1103	38-39	0.5535976253234551
1103	40-41	0.47764998872518305
1103	42-43	0.515355185270522
1103	44-45	0.47229390806766247
1103	46-47	0.4768697573457388
1103	48-49	0.48869546373398975
1103	50-51	0.42066463643966756
1103	52-53	0.472999288655231
1103	54-55	0.44894088334921634
1103	56-57	0.4304501170201007
1103	58-59	0.42324644279768364
1103	60-61	0.4602677853553985
1103	62-63	0.4192998835622106
1103	64-65	0.38529395414933276
1103	66-67	0.40375585654541624
1103	68-69	0.48970353934652877
1103	70-71	0.4035245574622053
1103	72-73	0.4428334283885391
1103	74-75	0.4250943586803899
1103	76-77	0.42537126593968466
1103	78-79	0.408412888812304
1103	80-81	0.3811199300341457
1103	82-83	0.45364663283108797
1103	84-85	0.4033269097919465
1103	86-87	0.3512134449291757
1103	88-89	0.3993579612758609
1103	90-91	0.36671181332521385
1103	92-93	0.4113076708279131
1103	94-95	0.45373848365088065
1103	96-97	0.4806333018009994
1103	98-99	0.4604986086229239
1103	100	0.47267212795728497
1104	1	1.572607056162738
1104	2	1.7642833365157564
1104	3	1.8054007621596746
1104	4	0.8898030955698459
1104	5	1.5245920195637197
1104	6	1.0376627132492118
1104	7	0.48660830197150773
1104	8	0.3533411801451365
1104	9	0.35714572054229166
1104	10-11	0.25323694739246605
1104	12-13	0.2645226133608318
1104	14-15	0.3778219108328855
1104	16-17	0.3160752218635636
1104	18-19	0.3692503196915524
1104	20-21	0.4398257143305244
1104	22-23	0.37241863008995324
1104	24-25	0.41779035639207507
1104	26-27	0.39828894020158145
1104	28-29	0.49395256595387593
1104	30-31	0.5302383774814245
1104	32-33	0.45698112169704075
1104	34-35	0.432501262622786
1104	36-37	0.4663718521909175
1104	38-39	0.4820288372766157
1104	40-41	0.5056748856570081
1104	42-43	0.4594833896927639
1104	44-45	0.4729821861016035
1104	46-47	0.5537518674922808
1104	48-49	0.5118108124074112
1104	50-51	0.5192281794754976
1104	52-53	0.5669395421577974
1104	54-55	0.46310553268912
1104	56-57	0.43421794663718316
1104	58-59	0.35549698278880015
1104	60-61	0.3495498823294483
1104	62-63	0.3981691425101488
1104	64-65	0.43890417661864944
1104	66-67	0.4331741607693189
1104	68-69	0.31741600609813503
1104	70-71	0.31647996372439735
1104	72-73	0.2406638544808004
1104	74-75	0.3370987697678842
1104	76-77	0.3737254836082968
1104	78-79	0.3548788824634421
1104	80-81	0.34285980389444504
1104	82-83	0.38950564847126046
1104	84-85	0.3434779360932012
1104	86-87	0.3436068910137777
1104	88-89	0.3796666502529291
1104	90-91	0.35579495788241644
1104	92-93	0.33247710414168097
1104	94-95	0.3531713223448705
1104	96-97	0.349680549008923
1104	98-99	0.405269087622635
1104	100	0.36861412180184416
1105	1	2.2297642546423333
1105	2	2.0262649261158323
1105	3	1.9987767473847136
1105	4	1.0273371909898472
1105	5	1.625308733313645
1105	6	1.1315654139280085
1105	7	0.6033712361443975
1105	8	0.4487002514575664
1105	9	0.37617278292267287
1105	10-11	0.3189082496639699
1105	12-13	0.2875657383388628
1105	14-15	0.38780690689484487
1105	16-17	0.3312694324109131
1105	18-19	0.31324045068828354
1105	20-21	0.37830859172103004
1105	22-23	0.393616801069939
1105	24-25	0.4819814734477319
1105	26-27	0.34462075080397625
1105	28-29	0.4585852068663385
1105	30-31	0.49666531945167236
1105	32-33	0.4667437481584784
1105	34-35	0.4897430667253886
1105	36-37	0.4564050175650749
1105	38-39	0.45402288858930007
1105	40-41	0.4154237041682549
1105	42-43	0.39650352961733404
1105	44-45	0.4178646126828198
1105	46-47	0.4465641795862183
1105	48-49	0.3950154806364736
1105	50-51	0.46701275471801296
1105	52-53	0.4777161063851949
1105	54-55	0.38516091218539117
1105	56-57	0.4231535565143574
1105	58-59	0.33508183827709814
1105	60-61	0.39787951100527863
1105	62-63	0.3849442176124782
1105	64-65	0.4074785440748059
1105	66-67	0.37437096147176874
1105	68-69	0.28280318237382573
1105	70-71	0.37375181777301236
1105	72-73	0.3629924706236949
1105	74-75	0.3980339751727868
1105	76-77	0.3253708195712015
1105	78-79	0.2732897857257548
1105	80-81	0.3449863822302959
1105	82-83	0.4331572893635851
1105	84-85	0.4582761672331763
1105	86-87	0.39580203205770914
1105	88-89	0.4007920440199122
1105	90-91	0.4673985416990796
1105	92-93	0.46829068990257383
1105	94-95	0.4500882767648129
1105	96-97	0.47963715613563096
1105	98-99	0.5198553351746931
1105	100	0.594049981243824
1106	1	1.699027715606075
1106	2	1.1206428226751513
1106	3	1.5777553172654741
1106	4	0.826730711675765
1106	5	1.5143709974277755
1106	6	1.022234782937467
1106	7	0.5837189949159196
1106	8	0.45606737302528444
1106	9	0.36001570554323337
1106	10-11	0.2708590171233638
1106	12-13	0.2411122221511377
1106	14-15	0.345983190821876
1106	16-17	0.34765878287300467
1106	18-19	0.329854848652154
1106	20-21	0.38801038282883127
1106	22-23	0.35614972514441945
1106	24-25	0.42297954403424853
1106	26-27	0.3492039147457291
1106	28-29	0.4167377967565926
1106	30-31	0.3616673620537014
1106	32-33	0.36162372355776284
1106	34-35	0.33400086092645864
1106	36-37	0.29557181236049246
1106	38-39	0.3262516949336103
1106	40-41	0.38689417663028536
1106	42-43	0.38448426181734874
1106	44-45	0.40452026687957954
1106	46-47	0.3577003823725491
1106	48-49	0.23958776182644925
1106	50-51	0.2152154740011838
1106	52-53	0.29001086504419504
1106	54-55	0.3258040948828409
1106	56-57	0.33150557243674683
1106	58-59	0.29174461975748756
1106	60-61	0.31020882407920425
1106	62-63	0.29048208068324755
1106	64-65	0.2559738847121622
1106	66-67	0.2816503820931544
1106	68-69	0.31500137022748476
1106	70-71	0.2902664843817817
1106	72-73	0.23864923741319188
1106	74-75	0.2744271368537099
1106	76-77	0.0367567376989868
1106	78-79	0.07621153554577376
1106	80-81	0.10567704176224524
1106	82-83	0.10268263912204745
1106	84-85	0.05778811408928419
1106	86-87	0.06093661271947326
1106	88-89	0.053853110008024174
1106	90-91	0.11984298505133495
1106	92-93	0.09906629475705131
1106	94-95	0.007207181290461051
1106	96-97	0.042715752776707916
1106	98-99	0.05765747448946712
1106	100	0.062319869820228746
1107	1	1.7045573630107427
1107	2	1.1630681956151143
1107	3	1.4191535347551572
1107	4	0.8028913650030347
1107	5	1.5341997228263793
1107	6	1.0985539686421788
1107	7	0.6581514010461973
1107	8	0.5080715251150636
1107	9	0.4738126386081163
1107	10-11	0.25809026334123786
1107	12-13	0.21161153986557935
1107	14-15	0.31044829133617924
1107	16-17	0.3133721597114416
1107	18-19	0.3287996186374116
1107	20-21	0.29151653309833847
1107	22-23	0.2914752688488562
1107	24-25	0.3100265004047955
1107	26-27	0.29700229770111264
1107	28-29	0.2934158716713213
1107	30-31	0.23174682762032006
1107	32-33	0.2575314651144254
1107	34-35	0.2587804091361292
1107	36-37	0.36369807497496254
1107	38-39	0.2895914329079332
1107	40-41	0.2734863627766728
1107	42-43	0.23194633376331808
1107	44-45	0.25943683620731406
1107	46-47	0.2745089456356027
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2210	5	1.6004601332901558
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2212	60-61	-0.10399585906952069
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2212	98-99	-0.04439682728580863
2212	100	-0.10243636982779236
2213	1	-2.7566720169867587
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2213	4	-1.414572244884127
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2213	98-99	-0.21760574839625235
2213	100	-0.2063647841543741
2214	1	-2.6558536606018883
2214	2	-2.747151615487258
2214	3	-2.7652069305101143
2214	4	-1.4283470445803985
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2214	7	-0.803059665618072
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2214	94-95	-0.4906982789528449
2214	96-97	-0.5370232747947412
2214	98-99	-0.6642506974597495
2214	100	-0.6645745391713689
2215	1	-5.641568080460363
2215	2	-4.387598858010268
2215	3	-3.5015424542730145
2215	4	-1.8089364023563945
2215	5	-2.455866771580105
2215	6	-4.9184250983389255
2215	7	-2.009917072993055
2215	8	-1.03867864837202
2215	9	-1.4704839482995098
2215	10-11	-0.7545960157064755
2215	12-13	-0.5657169956739807
2215	14-15	-0.7167931873192117
2215	16-17	-0.5792218176786648
2215	18-19	-0.6835912160194724
2215	20-21	-0.6388193618946261
2215	22-23	-0.7389450880040442
2215	24-25	-0.8535197996820401
2215	26-27	-0.793614456587342
2215	28-29	-0.7825068139068918
2215	30-31	-0.9058066153148232
2215	32-33	-0.8732573851786327
2215	34-35	-0.8337436614788913
2215	36-37	-0.8360325544490479
2215	38-39	-0.8472467013084852
2215	40-41	-0.8956916076919512
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2215	44-45	-0.8863481030135034
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2215	62-63	-0.7018751096351536
2215	64-65	-0.6784149992344624
2215	66-67	-0.718605560282036
2215	68-69	-0.689589253696802
2215	70-71	-0.7038956706957151
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2215	84-85	-0.5972333434982708
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2215	88-89	-0.7012067549455523
2215	90-91	-0.6782908805311401
2215	92-93	-0.6623964166286669
2215	94-95	-0.6521502208367984
2215	96-97	-0.6411387749707416
2215	98-99	-0.6702021892536969
2215	100	-0.7059624659986845
2216	1	-5.886532681389021
2216	2	-4.890996004882762
2216	3	-5.925268409231741
2216	4	-3.213815638308244
2216	5	-4.424255576062706
2216	6	-2.1728048204538766
2216	7	-1.4751131525743446
2216	8	-1.0071359100119963
2216	9	-0.8340912632995838
2216	10-11	-1.1370093701135175
2216	12-13	-0.8647492438170872
2216	14-15	-1.7140535977873625
2216	16-17	-1.3055003142038473
2216	18-19	-1.7895392046655303
2216	20-21	-1.2342690163190966
2216	22-23	-0.9693892500309005
2216	24-25	-1.6154836607074188
2216	26-27	-1.313352896627265
2216	28-29	-2.081602222481436
2216	30-31	-2.166525364082567
2216	32-33	-1.3778171401224881
2216	34-35	-1.3959768773086907
2216	36-37	-1.4259495505646242
2216	38-39	-1.3198011712927666
2216	40-41	-1.2895707726360186
2216	42-43	-1.7258108222156068
2216	44-45	-1.5797233845545051
2216	46-47	-1.9352430977347126
2216	48-49	-1.5851870603406581
2216	50-51	-1.4692110331347692
2216	52-53	-1.3635608451055745
2216	54-55	-1.3095495684048117
2216	56-57	-1.1186774726979323
2216	58-59	-1.0394578186367838
2216	60-61	-1.1192043153517162
2216	62-63	-1.008682997999749
2216	64-65	-1.1382463611617766
2216	66-67	-1.2285745663366114
2216	68-69	-1.1747919142506227
2216	70-71	-1.1202692115221886
2216	72-73	-1.1303309999332356
2216	74-75	-1.158190069108592
2216	76-77	-1.1053705976713033
2216	78-79	-1.059923253929778
2216	80-81	-1.0961997789475184
2216	82-83	-1.0687394761657742
2216	84-85	-1.065958792615021
2216	86-87	-1.0248536988759014
2216	88-89	-1.0521063400806554
2216	90-91	-1.1106860026579994
2216	92-93	-1.1745852628371765
2216	94-95	-1.1492175195657701
2216	96-97	-1.1190920980993582
2216	98-99	-1.1977595555882559
2216	100	-1.259461762418475
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	10194.0
3	7149.0
4	7938.0
5	10446.0
6	8730.0
7	6581.0
8	6064.0
9	6399.0
10	6834.0
11	12446.0
12	8784.0
13	11329.0
14	10733.0
15	11761.0
16	13755.0
17	17670.0
18	24109.0
19	39539.0
20	80026.0
21	106349.0
22	50068.0
23	31911.0
24	30394.0
25	33054.0
26	33606.0
27	37638.0
28	43508.0
29	51028.0
30	59417.0
31	73432.0
32	94483.0
33	127558.0
34	182985.0
35	397652.0
36	497790.0
37	862113.0
38	1205213.0
39	490863.0
40	3405.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	14.353743850762726	43.482259645526234	26.19528901176335	15.968707491947686
2	17.071707247161488	41.716578817459435	24.683620767446428	16.52809316793265
3	18.068391925743388	26.163251328147908	21.53549557241594	34.23286117369276
4	32.67706410883705	27.1358048476603	22.67463251285712	17.512498530645534
5	16.940670331176584	40.76366966450341	23.95680076656806	18.33885923775195
6	35.12854146253072	24.997400780911505	23.84659387721586	16.027463879341916
7	17.89404267472163	24.907287446472	39.82918144331559	17.369488435490776
8	34.21051849858921	25.3342807928955	23.41887911488209	17.036321593633208
9	33.8816801521933	26.561303165700323	23.37440594582506	16.182610736281323
10-11	16.301686797706914	43.63681461775354	23.44246304971362	16.61903553482593
12-13	16.22011375455818	26.23731315858377	31.921879568525384	25.620693518332665
14-15	16.517305282419475	34.663419163437624	23.361600813417656	25.457674740725245
16-17	25.417676429097412	25.96458459072914	31.81048976028488	16.80724921988857
18-19	16.8164064406315	26.163919698770666	31.837813396863197	25.181860463734633
20-21	25.581824647586682	34.86974774938403	23.18345564926462	16.364971953764663
22-23	25.22332371549809	26.321702637978735	31.87206387173372	16.58290977478946
24-25	16.689691857802984	34.80159152837053	23.139001568867425	25.36971504495906
26-27	17.01445140487805	29.902708118626837	36.51905256906202	16.563787907433095
28-29	24.69370029144423	26.18914625649957	32.54246289738067	16.574690554675524
30-31	22.436002648601626	27.437747411210466	32.93191945849019	17.194330481697712
32-33	18.90826296594612	28.714795441602213	33.94289993133716	18.434041661114513
34-35	18.994190672037938	28.5371075239693	34.01936173885385	18.44934006513891
36-37	18.919490992681403	28.508019325701216	34.48660708365017	18.08588259796721
38-39	19.44332298359069	28.421073070569484	33.99927064076554	18.136333305074288
40-41	18.511898244693807	29.55996822268564	33.86127895202763	18.066854580592924
42-43	18.595211605985057	28.49402973261274	34.32687837273796	18.583880288664247
44-45	18.55452318090029	29.18732907166699	33.726689083311705	18.53145866412101
46-47	19.11565157038107	28.63618028800911	34.04082296926458	18.207345172345235
48-49	18.58091219040619	28.891900081225806	33.943792931063314	18.583394797304695
50-51	19.072042056867552	29.473335304695947	33.30534380383647	18.149278834600025
52-53	19.18477048282702	29.159943392409026	33.140945750775735	18.51434037398822
54-55	19.205329559848114	30.129127925795764	31.53070057201699	19.134841942339133
56-57	19.593139493742466	30.65977709136644	30.575254075633616	19.171829339257478
58-59	20.72535204618207	30.903826647285864	29.332770199482844	19.03805110704922
60-61	20.290372799146294	31.2829573031543	29.25821804654151	19.168451851157894
62-63	19.596300464165985	31.46336891079867	29.21387568889941	19.726454936135934
64-65	19.99156508965673	31.27841941451112	29.577601970904333	19.152413524927812
66-67	19.84845649028678	30.688993095299633	30.126980873186344	19.335569541227247
68-69	20.266240640897582	30.745305302552357	29.824106276410422	19.164347780139636
70-71	19.058717752620915	31.639545750471726	29.86676218616286	19.4349743107445
72-73	19.049216035036924	30.767223119022525	30.2894439914763	19.894116854464254
74-75	19.28672989653622	31.1145871814061	29.665922390600862	19.93276053145682
76-77	19.92074648955753	30.596364099326824	29.985464884965708	19.497424526149945
78-79	19.663144236832746	31.10889956369039	29.31487220603058	19.913083993446286
80-81	20.075831886039122	31.436132123947445	29.012444378332276	19.47559161168116
82-83	20.14318007192106	31.259051403377324	29.11442713860326	19.48334138609836
84-85	19.93195030696098	31.741489677167124	28.567365111429517	19.75919490444238
86-87	19.929648319047015	31.946865107325355	28.67252209205961	19.450964481568015
88-89	20.943515433406944	31.47880297002969	28.16694507821605	19.410736518347314
90-91	20.376959242410532	32.097937214059705	28.08616587144571	19.43893767208406
92-93	19.92433636492213	32.148174760163826	28.042229967922104	19.885258906991936
94-95	20.221984935046525	32.28331636083995	27.910524438374573	19.584174265738955
96-97	20.106426457880985	31.56049626776356	28.334089485397847	19.998987788957606
98-99	20.648383507748562	31.81266256772583	27.545122175179383	19.993831749346228
100	19.99079955983872	32.59251683387731	27.483323141271317	19.933360465012655
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	161576.0
1	87335.0
2	9556.0
3	4967.0
4	3744.0
5	3411.5
6	3421.0
7	3969.0
8	3795.5
9	3269.5
10	3434.5
11	5262.0
12	45309.5
13	50052.5
14	14709.5
15	13607.5
16	15043.5
17	16693.0
18	18461.0
19	20610.0
20	23016.0
21	26168.0
22	30064.0
23	35321.5
24	41212.0
25	47033.0
26	55645.5
27	64781.5
28	70972.5
29	75898.5
30	81156.0
31	87716.5
32	95381.0
33	104061.0
34	114601.0
35	122227.5
36	128255.5
37	134444.5
38	137812.0
39	139238.5
40	140152.0
41	142321.0
42	143290.5
43	142190.5
44	141519.5
45	141039.5
46	140339.5
47	141644.5
48	140657.5
49	146150.5
50	179204.5
51	172614.0
52	126331.0
53	107012.0
54	100089.0
55	96276.5
56	88196.0
57	76866.0
58	71722.0
59	61518.0
60	45991.5
61	36096.5
62	27666.0
63	20603.0
64	15116.0
65	11050.0
66	8509.0
67	6659.5
68	5210.0
69	3999.0
70	2865.0
71	2082.0
72	1640.5
73	1163.5
74	887.0
75	663.5
76	397.5
77	256.5
78	202.0
79	140.0
80	68.0
81	35.0
82	27.5
83	16.5
84	8.0
85	7.0
86	6.5
87	4.0
88	2.5
89	0.5
90	0.0
91	0.0
92	0.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.059771429977886474
2	0.06745238761082753
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	1.1669963254468428E-4
18-19	0.0
20-21	4.667985301787371E-4
22-23	1.8035397756905754E-4
24-25	0.0
26-27	0.0026522643760155517
28-29	9.65424232869661E-4
30-31	0.00590924502976265
32-33	0.0014852680505687093
34-35	0.0021748567883327526
36-37	0.007840093495501972
38-39	0.012699041832362463
40-41	0.003352462171283658
42-43	0.00744755836785167
44-45	0.0018459760057068242
46-47	0.0024825194559505567
48-49	0.0035222070913486533
50-51	0.010131649916379408
52-53	0.00336307122878772
54-55	0.001474658993064647
56-57	7.744611977965411E-4
58-59	9.017698878452877E-4
60-61	0.006842842090120124
62-63	0.0019626756382515086
64-65	0.00841298260072133
66-67	0.010863674884159702
68-69	0.011945798749574046
70-71	0.01014225897388347
72-73	0.004487631324218314
74-75	0.0020581571557880683
76-77	0.004858948336860491
78-79	0.017367027134149834
80-81	0.009007089820948815
82-83	0.0060895990073317075
84-85	0.004699812474299558
86-87	0.007309640620298861
88-89	0.004381540749177692
90-91	0.006842842090120124
92-93	0.01100159263171251
94-95	0.009060135108469126
96-97	0.01056662127404596
98-99	0.002344601708397748
100	0.0028856636411049206
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4712954.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.90406956971306
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.86961743335154	46.24775884367154
2	14.371481162530733	16.643344901100004
3	3.458301671716578	6.00749221776395
4	0.987706392573905	2.287688786801988
5	0.386814362922084	1.1199062890602292
6	0.1959761680871564	0.6808690602554689
7	0.136320147367293	0.5525443907836489
8	0.08743651608938109	0.4050344088458286
9	0.06327585465794083	0.3297536541176812
>10	0.30240786049588325	3.456826224297955
>50	0.11332721386772078	4.647913283687716
>100	0.022673844201485778	2.115600357214619
>500	0.0019977311318577413	0.8113867626056618
>1k	0.002367680894721142	2.5078503149036915
>5k	1.1098504194005228E-4	0.4260545729781003
>10k+	1.8497506990008714E-4	11.759975931912042
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	329341	6.987995214890703	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	163178	3.4623295707957262	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	32912	0.6983306011473908	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	13029	0.276450820440853	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA	10517	0.2231509155404445	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7172	0.1521763208382683	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6675	0.14163091767923047	No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6042	0.12819985087908772	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0022915564208774368	0.0	0.0	0.0	0.0
2	0.0022915564208774368	0.0	0.0	0.0	0.0
3	0.0022915564208774368	0.0	0.0	0.0	0.0
4	0.0022915564208774368	0.0	0.0	0.0	0.0
5	0.0022915564208774368	0.0	0.0	0.0	0.0
6	0.0022915564208774368	0.0	0.0	0.0	0.0
7	0.0023127745358855613	0.0	0.0	0.0	0.0
8	0.0023127745358855613	0.0	0.0	0.0	0.0
9	0.0023127745358855613	0.0	0.0	0.0	0.0
10-11	0.0023233835933896235	0.0	0.0	0.0	0.0
12-13	0.0023764288809099347	0.0	0.0	0.0	0.0
14-15	0.002387037938413997	0.0	0.0	0.0	0.0
16-17	0.002408256053422121	0.0	0.0	0.0	0.0
18-19	0.002461301340942432	0.0	0.0	0.0	0.0
20-21	0.002503737570958681	0.0	0.0	0.0	0.0
22-23	0.002599219088495241	0.0	0.0	0.0	0.0
24-25	0.002747745893552112	0.0	0.0	0.0	0.0
26-27	0.0029387089286252313	0.0	0.0	0.0	0.0
28-29	0.0030341904461617915	0.0	0.0	0.0	0.0
30-31	0.0031296719636983517	0.0	0.0	0.0	0.0
32-33	0.0033418531137795957	0.0	0.0	0.0	2.1218115008124415E-5
34-35	0.0034797708613324044	0.0	0.0	0.0	2.1218115008124415E-5
36-37	0.0035964704938770886	0.0	0.0	0.0	2.1218115008124415E-5
38-39	0.003978396564023328	0.0	0.0	0.0	2.1218115008124415E-5
40-41	0.004551285669242688	0.0	0.0	0.0	2.1218115008124415E-5
42-43	0.0055273189596164105	0.0	0.0	0.0	6.365434502437325E-5
44-45	0.006842842090120124	0.0	0.0	0.0	6.365434502437325E-5
46-47	0.007999229358062906	0.0	0.0	0.0	6.365434502437325E-5
48-49	0.009505715523639739	0.0	0.0	0.0	6.365434502437325E-5
50-51	0.011266819069314066	0.0	0.0	0.0	6.365434502437325E-5
52-53	0.0140357830778743	0.0	0.0	0.0	6.365434502437325E-5
54-55	0.01728215467411734	0.0	0.0	0.0	6.365434502437325E-5
56-57	0.020868016110490363	0.0	0.0	0.0	6.365434502437325E-5
58-59	0.025769400677367105	0.0	0.0	0.0	6.365434502437325E-5
60-61	0.03252737030745473	0.0	0.0	0.0	6.365434502437325E-5
62-63	0.0414071514383548	0.0	0.0	1.0609057504062208E-5	8.487246003249766E-5
64-65	0.052599707105140434	0.0	0.0	2.1218115008124415E-5	8.487246003249766E-5
66-67	0.06765395970340471	0.0	0.0	3.182717251218662E-5	8.487246003249766E-5
68-69	0.08732315231593604	0.0	0.0	4.243623001624883E-5	8.487246003249766E-5
70-71	0.11466269350390434	0.0	0.0	4.243623001624883E-5	8.487246003249766E-5
72-73	0.14793269783664342	0.0	0.0	4.243623001624883E-5	8.487246003249766E-5
74-75	0.19096303507311974	0.0	0.0	4.243623001624883E-5	8.487246003249766E-5
76-77	0.24521775514889388	0.0	0.0	4.243623001624883E-5	8.487246003249766E-5
78-79	0.3143569829028673	0.0	0.0	4.243623001624883E-5	8.487246003249766E-5
80-81	0.4035473293395183	0.0	0.0	4.243623001624883E-5	8.487246003249766E-5
82-83	0.5121840781811153	0.0	0.0	4.243623001624883E-5	8.487246003249766E-5
84-85	0.6473116436103556	0.0	0.0	4.243623001624883E-5	8.487246003249766E-5
86-87	0.8064050699412725	0.0	0.0	4.243623001624883E-5	8.487246003249766E-5
88	0.9452882417269509	0.0	0.0	4.243623001624883E-5	8.487246003249766E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	3430	0.0	84.64565	1
AACGAGT	99460	0.0	69.46723	1
GTGGAAT	109500	0.0	69.12972	6
ACGAGTG	105080	0.0	68.82114	2
TGGAATC	111035	0.0	68.4703	7
GAATCAC	111515	0.0	68.2388	9
GGAATCA	111560	0.0	68.1902	8
CGAGTGG	107830	0.0	68.00823	3
AGTGGAA	109095	0.0	67.79256	5
GAGTGGA	111495	0.0	66.476585	4
AACGTGT	2190	0.0	62.526264	1
TCGAGTG	1685	0.0	62.51179	2
CGTGTGG	2625	0.0	62.299885	3
ATCGAGT	1520	0.0	61.915596	1
ACGTGTG	2745	0.0	56.016922	2
GACGAGT	1005	0.0	45.417088	1
GAGTACT	50460	0.0	41.164818	20-21
AGTACTT	51680	0.0	41.06147	20-21
GTACTTT	51130	0.0	39.972816	22-23
ACGATTG	800	0.0	39.969875	2
>>END_MODULE