FastQCFastQC Report
Thu 26 May 2016
SRR2132682_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132682_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2908352
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2395228.23566060779438No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1658135.701269997579385No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG741832.5506884998789694No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT96030.330186992496094No Hit
GTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGT71550.2460156129656933No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA71060.24433081002574655No Hit
AGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTC57400.19736263010804744No Hit
CCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGAT56250.19340850075919283No Hit
CACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCA53450.18378105538806858No Hit
TTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTG52220.17955185617146754No Hit
CCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATT50880.17494443588671524No Hit
CCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTC49880.17150606253988512No Hit
CTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCG49790.17119660893867042No Hit
ACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTC48830.16789577052571353No Hit
AACGAGTGGAATCACTGCTGAGTACATGGTAACGAGTGGAATCACTGCTG48090.16535137424905927No Hit
TCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGT47430.1630820478401514No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT47220.16235998943731708No Hit
TACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACT46320.15926545342516998No Hit
CGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAG44740.15383282353717845No Hit
TCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGA44590.15331706753515395No Hit
TCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCA44380.1525950091323196No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT43220.1486064960499967No Hit
ACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGA43140.1483314261822503No Hit
CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT43130.14829704244878197No Hit
ATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTA42610.14650908830843035No Hit
ACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAG42430.1458901811060009No Hit
TCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACT42290.14540880883744473No Hit
TGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCA41700.14338016856281494No Hit
GTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCAC40120.13794753867482343No Hit
CAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTT39470.13571259599938384No Hit
GATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGT39340.13526560746429594No Hit
CTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGC37660.12948914024162136No Hit
GAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGT37230.1280106397024844No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC37130.12766680236780142No Hit
ATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCC36010.12381582421935172No Hit
GTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCAT34620.11903648526725788No Hit
TGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATG33640.11566687938736439No Hit
AGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCA32800.11277864577602711No Hit
CTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACG32790.1127442620425588No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32200.11071562176792905No Hit
CGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAG30820.10597066654930351No Hit
TTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTAC30730.10566121294808882No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT30580.1051454569460643No Hit
CTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGT30290.10414832867548357No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT27950.089.061651
AACGTGT14700.071.70471
ATCGAGT9850.068.31511
ACGTGTG17300.068.2358862
CGTGTGG19950.065.721943
TCGAGTG12000.064.275852
GTGGAAT1230300.050.8297736
ATCTGCG8950.050.4146894
TGGAATC1248550.050.4029737
GGAATCA1258550.050.006228
AGTGGAA1222900.049.9383775
GAATCAC1263350.049.8459859
ACGAGTG1234150.048.4315832
AACGAGT1187550.048.0606841
CGAGTGG1261850.047.9760673
GAGTGGA1286900.047.527884
GTGTGGA30500.046.9947244
CGATTGG7550.044.8161623
ACGATTG7400.044.4888842
AGTACTT380050.039.55795320-21
Produced by FastQC (version 0.11.5)