##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132682_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2908352
Sequences flagged as poor quality	0
Sequence length	100
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.28596538520784	31.0	28.0	34.0	16.0	34.0
2	30.384943431881698	33.0	30.0	34.0	23.0	34.0
3	30.80550015954052	33.0	31.0	34.0	25.0	34.0
4	35.210154066632924	37.0	35.0	37.0	32.0	37.0
5	34.659483102458026	37.0	35.0	37.0	30.0	37.0
6	35.16478541799617	37.0	35.0	37.0	32.0	37.0
7	35.53918473417248	37.0	35.0	37.0	33.0	37.0
8	35.73877749323328	37.0	35.0	37.0	35.0	37.0
9	37.625401945844246	39.0	39.0	39.0	35.0	39.0
10-11	37.69961201395154	39.0	39.0	39.0	35.0	39.0
12-13	37.67155712238409	39.0	39.0	39.0	35.0	39.0
14-15	39.09225172881412	41.0	40.0	41.0	36.0	41.0
16-17	38.96611156421231	41.0	39.5	41.0	36.0	41.0
18-19	38.70376625662918	41.0	38.5	41.0	35.5	41.0
20-21	38.48474943885747	40.5	39.0	41.0	35.0	41.0
22-23	38.34805295232489	40.0	38.0	41.0	35.0	41.0
24-25	38.20958759462403	40.0	38.0	41.0	34.5	41.0
26-27	38.167935139900536	40.0	38.0	41.0	34.5	41.0
28-29	37.98423265134345	40.0	38.0	41.0	34.0	41.0
30-31	37.79837584996589	40.0	38.0	41.0	34.0	41.0
32-33	37.63629161807099	40.0	37.5	41.0	33.5	41.0
34-35	37.52933327877781	40.0	37.0	41.0	33.0	41.0
36-37	37.29292602821117	40.0	37.0	41.0	33.0	41.0
38-39	36.7899294858394	40.0	35.5	41.0	33.0	41.0
40-41	36.58810591015118	40.0	35.0	41.0	33.0	41.0
42-43	36.44115103673833	39.5	35.0	41.0	32.5	41.0
44-45	36.26646499460863	39.0	35.0	41.0	32.0	41.0
46-47	36.089117823427145	39.0	35.0	41.0	31.0	41.0
48-49	35.882790322491914	39.0	35.0	41.0	31.0	41.0
50-51	35.54575340261427	39.0	35.0	41.0	29.5	41.0
52-53	34.92972721321216	38.5	35.0	41.0	25.5	41.0
54-55	33.95386889207359	38.0	35.0	41.0	15.5	41.0
56-57	33.264948500044014	38.0	34.0	40.0	2.0	41.0
58-59	32.94224804975464	38.0	34.0	40.0	2.0	41.0
60-61	32.64831509390885	37.0	34.0	40.0	2.0	41.0
62-63	32.35468419916159	37.0	34.0	40.0	2.0	41.0
64-65	32.02633914326739	36.0	33.0	40.0	2.0	41.0
66-67	31.615065851726335	35.5	33.0	39.0	2.0	41.0
68-69	31.335607931914705	35.0	33.0	39.0	2.0	41.0
70-71	31.014292458409436	35.0	33.0	38.5	2.0	40.5
72-73	30.671479071309115	35.0	32.0	37.5	2.0	40.0
74-75	30.313775636511675	35.0	32.0	37.0	2.0	40.0
76-77	29.93124594271945	35.0	31.5	36.5	2.0	39.0
78-79	29.75431584622494	35.0	32.0	36.0	2.0	39.0
80-81	29.57662672881412	35.0	32.0	36.0	2.0	39.0
82-83	29.198640501562394	35.0	31.5	35.5	2.0	37.0
84-85	28.886009327619213	35.0	31.0	35.0	2.0	37.0
86-87	28.722433701285127	35.0	31.0	35.0	2.0	37.0
88-89	28.49658363224259	35.0	31.0	35.0	2.0	36.0
90-91	28.25002303710142	35.0	30.5	35.0	2.0	36.0
92-93	28.08176451818762	35.0	30.0	35.0	2.0	36.0
94-95	27.837551988205007	35.0	30.0	35.0	2.0	35.5
96-97	27.573610931551613	35.0	29.0	35.0	2.0	35.0
98-99	27.416204090839074	35.0	29.0	35.0	2.0	35.0
100	27.323654770811785	35.0	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	2.741950222322952
1101	2	2.145605782958981
1101	3	1.992335116065263
1101	4	0.9635035174358677
1101	5	1.5431082985792983
1101	6	0.9750454684046304
1101	7	0.4494455621584592
1101	8	0.35137233155642633
1101	9	0.30848284113448443
1101	10-11	0.3025609064180088
1101	12-13	0.24448618900417074
1101	14-15	0.4159352468221016
1101	16-17	0.45116288264568283
1101	18-19	0.5317387900070258
1101	20-21	0.5554924000117936
1101	22-23	0.5099234217843929
1101	24-25	0.4975010352085931
1101	26-27	0.526084270245029
1101	28-29	0.6274958884267079
1101	30-31	0.6171073903296502
1101	32-33	0.6273768518943683
1101	34-35	0.6692381001462167
1101	36-37	0.6571350515271561
1101	38-39	0.6107803377307519
1101	40-41	0.6419329810071162
1101	42-43	0.573118892506379
1101	44-45	0.6956196404099657
1101	46-47	0.732609743948899
1101	48-49	0.6793490267033278
1101	50-51	0.6418697072745445
1101	52-53	0.5719025440690757
1101	54-55	0.5366412877149003
1101	56-57	0.5836011201397042
1101	58-59	0.5303274864302736
1101	60-61	0.4881689672643006
1101	62-63	0.531347994999031
1101	64-65	0.4578846614840941
1101	66-67	0.40634754668369766
1101	68-69	0.4387096447996832
1101	70-71	0.433080116276674
1101	72-73	0.4800764740361565
1101	74-75	0.4740653978522822
1101	76-77	0.473916805004702
1101	78-79	0.5465933550280937
1101	80-81	0.5194857062225644
1101	82-83	0.4862511901750004
1101	84-85	0.46115806165743933
1101	86-87	0.47858609492645243
1101	88-89	0.44034399096079113
1101	90-91	0.34106903336742533
1101	92-93	0.4104365334762754
1101	94-95	0.44879779347754933
1101	96-97	0.45876322689040094
1101	98-99	0.49087694085547184
1101	100	0.5065276748118208
1102	1	2.714324650251829
1102	2	2.1988843096969646
1102	3	2.05359603575117
1102	4	1.0271577006296937
1102	5	1.4955694488989906
1102	6	0.9439851532788737
1102	7	0.42835693498371086
1102	8	0.3931687526560239
1102	9	0.41069333051306955
1102	10-11	0.30646206070438353
1102	12-13	0.30728871966413607
1102	14-15	0.4541007805819177
1102	16-17	0.4780235874657066
1102	18-19	0.5590990085655605
1102	20-21	0.5649610927053601
1102	22-23	0.5967440531982859
1102	24-25	0.6061225732661697
1102	26-27	0.5734791141940931
1102	28-29	0.7216051920617161
1102	30-31	0.7023163030855386
1102	32-33	0.6379836362854192
1102	34-35	0.6700695093320022
1102	36-37	0.7189927990214571
1102	38-39	0.7212356816629892
1102	40-41	0.6650030416905537
1102	42-43	0.5995275052451703
1102	44-45	0.5882352488792506
1102	46-47	0.6349333153090058
1102	48-49	0.5327007944922997
1102	50-51	0.6060887893788163
1102	52-53	0.5330287177410469
1102	54-55	0.5040271482220007
1102	56-57	0.5168109026014847
1102	58-59	0.4361678411543721
1102	60-61	0.3332132973888875
1102	62-63	0.35988161459557944
1102	64-65	0.3591251154539421
1102	66-67	0.28576474490436965
1102	68-69	0.32325045968513066
1102	70-71	0.30787155504189556
1102	72-73	0.2619786850196846
1102	74-75	0.25157803113339483
1102	76-77	0.1592257608921166
1102	78-79	0.2930495477771089
1102	80-81	0.36243214041435934
1102	82-83	0.36711310645132045
1102	84-85	0.31897614094948423
1102	86-87	0.40249384837584046
1102	88-89	0.4528957090651211
1102	90-91	0.5182449644589582
1102	92-93	0.5209147038190203
1102	94-95	0.49918744563955286
1102	96-97	0.4854027710270472
1102	98-99	0.5512719169337892
1102	100	0.4875195921286668
1103	1	2.4282256257185892
1103	2	2.0602842531704653
1103	3	1.916593597208795
1103	4	0.9604873666213862
1103	5	1.4893722871889779
1103	6	0.9149104208071108
1103	7	0.4383352402966523
1103	8	0.3197001855463597
1103	9	0.322008320976785
1103	10-11	0.25024829910819335
1103	12-13	0.1909036220065019
1103	14-15	0.37947218608672983
1103	16-17	0.4010729458810758
1103	18-19	0.41236412233440944
1103	20-21	0.48214631096467286
1103	22-23	0.44304825553460603
1103	24-25	0.5100450109828358
1103	26-27	0.4241145060369078
1103	28-29	0.4448753491632118
1103	30-31	0.4607010442288697
1103	32-33	0.4549197649373866
1103	34-35	0.4585637433135261
1103	36-37	0.4760720458653296
1103	38-39	0.4545243224984006
1103	40-41	0.4584717626514774
1103	42-43	0.5098105024513373
1103	44-45	0.44158835671054675
1103	46-47	0.48685710071838173
1103	48-49	0.416644318685492
1103	50-51	0.5209415838903766
1103	52-53	0.45555121862528836
1103	54-55	0.42692799590367514
1103	56-57	0.4978495933693665
1103	58-59	0.39884283519540986
1103	60-61	0.35673283741127193
1103	62-63	0.30018956946359054
1103	64-65	0.3088173603712008
1103	66-67	0.35187952646243303
1103	68-69	0.4320719142535445
1103	70-71	0.37828456983967484
1103	72-73	0.3468053762863157
1103	74-75	0.30218687533359656
1103	76-77	0.37121045811589326
1103	78-79	0.32839407856703673
1103	80-81	0.3974584536828516
1103	82-83	0.4624003547668565
1103	84-85	0.42374306155742403
1103	86-87	0.36945996418675975
1103	88-89	0.42344780428953044
1103	90-91	0.4331422817524313
1103	92-93	0.4093896178463332
1103	94-95	0.5018567648936916
1103	96-97	0.4348099207161802
1103	98-99	0.40682451660214625
1103	100	0.45223662951136845
1104	1	1.4392630393682744
1104	2	1.7029080843261468
1104	3	1.7666240887995812
1104	4	0.8841568573395264
1104	5	1.5320560489922457
1104	6	1.0415147377699654
1104	7	0.49696244679063994
1104	8	0.35062804635501266
1104	9	0.3107854380797974
1104	10-11	0.26704916031489034
1104	12-13	0.23934089076797704
1104	14-15	0.42034731706873174
1104	16-17	0.41413281954297787
1104	18-19	0.4663665848386813
1104	20-21	0.5097989305197785
1104	22-23	0.43746599798315344
1104	24-25	0.4247513773888443
1104	26-27	0.3972907835792725
1104	28-29	0.44876371804596715
1104	30-31	0.4753192795184873
1104	32-33	0.42692443393564616
1104	34-35	0.3188140210123933
1104	36-37	0.4486830913909401
1104	38-39	0.46092030045769405
1104	40-41	0.4753430585823608
1104	42-43	0.49125512555486495
1104	44-45	0.5478959643411869
1104	46-47	0.5354013771023318
1104	48-49	0.5188985807039401
1104	50-51	0.467655190029177
1104	52-53	0.4924297238824238
1104	54-55	0.47500822706140156
1104	56-57	0.4598786056714772
1104	58-59	0.4563649873148492
1104	60-61	0.5293457275292752
1104	62-63	0.5111604927336515
1104	64-65	0.49361242775038505
1104	66-67	0.5114899538635314
1104	68-69	0.4628984553759423
1104	70-71	0.41795255596578684
1104	72-73	0.49549260060746647
1104	74-75	0.4916117367393227
1104	76-77	0.4195640823200044
1104	78-79	0.4879750297005394
1104	80-81	0.5959461643384962
1104	82-83	0.5920655522094762
1104	84-85	0.5009346552767546
1104	86-87	0.4966288486878483
1104	88-89	0.5462395560803266
1104	90-91	0.4925781054591525
1104	92-93	0.4659267735720327
1104	94-95	0.48282838560041696
1104	96-97	0.49458460189406495
1104	98-99	0.49494345974745
1104	100	0.4640077869330277
1105	1	2.1735524752436923
1105	2	1.967664954227395
1105	3	1.9318245710138058
1105	4	0.9246005109379638
1105	5	1.5494248437869729
1105	6	1.0662199595554753
1105	7	0.5519167024962854
1105	8	0.3612654526370065
1105	9	0.2504009052661118
1105	10-11	0.22721271268137144
1105	12-13	0.19177580423364304
1105	14-15	0.23797424922845778
1105	16-17	0.26856997480935263
1105	18-19	0.25254885603463606
1105	20-21	0.36622418731536754
1105	22-23	0.3527160227544286
1105	24-25	0.4166763534432718
1105	26-27	0.3041860753849406
1105	28-29	0.4223713657653576
1105	30-31	0.45992898738253984
1105	32-33	0.4759936531449469
1105	34-35	0.43478984424455547
1105	36-37	0.4674700418814055
1105	38-39	0.4740741589971904
1105	40-41	0.35174604187000824
1105	42-43	0.45099127015307516
1105	44-45	0.4244139276466612
1105	46-47	0.4773087036208068
1105	48-49	0.4281401254408692
1105	50-51	0.3944919803320488
1105	52-53	0.35809858968815433
1105	54-55	0.36140982728598914
1105	56-57	0.3150734524198313
1105	58-59	0.3289159335776901
1105	60-61	0.34745755746618556
1105	62-63	0.4047841592131576
1105	64-65	0.39914787957849995
1105	66-67	0.41222824117733126
1105	68-69	0.2991035367908772
1105	70-71	0.31638040548852686
1105	72-73	0.3259823287839687
1105	74-75	0.4025069707453319
1105	76-77	0.39496785416001856
1105	78-79	0.452671003542072
1105	80-81	0.4635824889413307
1105	82-83	0.44649352146006294
1105	84-85	0.352297591664108
1105	86-87	0.3553843602973181
1105	88-89	0.35019367141539703
1105	90-91	0.3864400922808322
1105	92-93	0.4085863212484888
1105	94-95	0.4061074010246628
1105	96-97	0.44703984845086
1105	98-99	0.4415966535909597
1105	100	0.34989393813691194
1106	1	1.3787615420513823
1106	2	0.9535506545350962
1106	3	1.4167933107699184
1106	4	0.7449747821677448
1106	5	1.4231850554831453
1106	6	0.9361881397665996
1106	7	0.49831877865580765
1106	8	0.3465863591197049
1106	9	0.17348070034390162
1106	10-11	0.1242768345572074
1106	12-13	0.052456626893530256
1106	14-15	0.20615044945247973
1106	16-17	0.1393447663664631
1106	18-19	0.17049126053318275
1106	20-21	0.29057051001535683
1106	22-23	0.19505302927782964
1106	24-25	0.29303372047210985
1106	26-27	0.29243122343658
1106	28-29	0.3372177533329648
1106	30-31	0.29459567187542035
1106	32-33	0.29765289717489907
1106	34-35	0.31631183634357996
1106	36-37	0.29104216227964486
1106	38-39	0.22064714396672258
1106	40-41	0.2899433810107226
1106	42-43	0.2929060182736478
1106	44-45	0.26746284376344676
1106	46-47	0.24644999516949895
1106	48-49	0.25352207679710403
1106	50-51	0.19095620064818775
1106	52-53	0.1608377852704166
1106	54-55	0.18546996500338508
1106	56-57	-0.008405080364056516
1106	58-59	-0.017237863058895186
1106	60-61	0.024333345409530693
1106	62-63	0.04343762655999228
1106	64-65	0.052702302509352705
1106	66-67	-9.963819715892441E-4
1106	68-69	0.007853557835712621
1106	70-71	0.026050927568768145
1106	72-73	0.027829792856724822
1106	74-75	0.04195117915752533
1106	76-77	-0.18321999252267318
1106	78-79	-0.19055055448868785
1106	80-81	-0.1512315390993031
1106	82-83	-0.14198304548875385
1106	84-85	-0.17910429632983593
1106	86-87	-0.11999719492085958
1106	88-89	-0.20175854641299296
1106	90-91	-0.18549729017757954
1106	92-93	-0.18546356144513254
1106	94-95	-0.19181017552158508
1106	96-97	-0.1652719488373755
1106	98-99	-0.10733204056966983
1106	100	-0.12105151464392705
1107	1	1.4440579478178392
1107	2	0.9664419160778159
1107	3	1.3165239976777698
1107	4	0.6816463640434876
1107	5	1.4046383046857684
1107	6	0.9811997166404964
1107	7	0.5455713310087802
1107	8	0.4453051393786325
1107	9	0.3965925408188511
1107	10-11	0.19528185153576771
1107	12-13	0.1056845664536965
1107	14-15	0.15339527627347138
1107	16-17	0.16119685960482855
1107	18-19	0.2438669876637789
1107	20-21	0.2290157512257025
1107	22-23	0.21151445764348864
1107	24-25	0.28201417492379477
1107	26-27	0.30106608813886027
1107	28-29	0.30512035755600664
1107	30-31	0.25620003971889105
1107	32-33	0.2476169636931118
1107	34-35	0.23844823201495302
1107	36-37	0.33935536876955297
1107	38-39	0.32226080910474764
1107	40-41	0.31406553352386624
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2208	94-95	0.31265944888583874
2208	96-97	0.3600019583542995
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2208	100	0.42007264006970857
2209	1	1.0661388797447628
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2209	4	0.9417526602592332
2209	5	1.5439668850467925
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2214	96-97	-0.5619193312149555
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2215	30-31	-1.0923043091769955
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2216	2	-4.797748481453137
2216	3	-5.868463029430451
2216	4	-2.997501944711914
2216	5	-4.0660973458325
2216	6	-1.9249371301368967
2216	7	-1.3763451625283238
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2216	12-13	-0.7277770092096674
2216	14-15	-1.6473146466228243
2216	16-17	-1.0575611464909542
2216	18-19	-1.6174048808631696
2216	20-21	-1.1400521601589944
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2216	30-31	-2.1823720772612845
2216	32-33	-1.461766658395426
2216	34-35	-1.542316798883867
2216	36-37	-1.513468144106909
2216	38-39	-1.4144243839209523
2216	40-41	-1.45387758113241
2216	42-43	-1.8421206289136336
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2216	54-55	-1.2967331615494047
2216	56-57	-1.3123185317219779
2216	58-59	-1.2821665246027578
2216	60-61	-1.3104504505593084
2216	62-63	-1.350236497005902
2216	64-65	-1.4617992637979498
2216	66-67	-1.449814023234321
2216	68-69	-1.5028741681495426
2216	70-71	-1.3689844072398003
2216	72-73	-1.4497391828054056
2216	74-75	-1.420399741873137
2216	76-77	-1.3772332130947014
2216	78-79	-1.3487386302694588
2216	80-81	-1.3519614916335918
2216	82-83	-1.368235909183447
2216	84-85	-1.3445247949777333
2216	86-87	-1.4025529701198423
2216	88-89	-1.4731653290525557
2216	90-91	-1.4962199306549024
2216	92-93	-1.5040408344996052
2216	94-95	-1.745669566035847
2216	96-97	-1.7665673981966457
2216	98-99	-1.761805145518526
2216	100	-1.8233791035390432
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	7591.0
3	6937.0
4	7645.0
5	10032.0
6	8380.0
7	5999.0
8	5094.0
9	5333.0
10	5712.0
11	10610.0
12	7420.0
13	9897.0
14	9663.0
15	10486.0
16	12190.0
17	15609.0
18	21121.0
19	32685.0
20	64058.0
21	82347.0
22	37584.0
23	22256.0
24	22216.0
25	24366.0
26	25248.0
27	27569.0
28	31070.0
29	34949.0
30	40150.0
31	48053.0
32	61428.0
33	83241.0
34	119524.0
35	304461.0
36	322822.0
37	528633.0
38	616170.0
39	217083.0
40	2720.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	13.500620635938537	48.04324222416104	22.993529423097563	15.462607716802859
2	15.879321355527235	46.725762009232476	22.19209954773551	15.202817087504783
3	16.43487445811236	25.171368527606013	19.40865479831877	38.985102215962854
4	37.67425676121735	26.343475617806924	20.166816121294808	15.815451499680918
5	15.618845311709173	46.26362283520014	21.63665195959774	16.480879893492947
6	39.736833780780316	24.294205103096186	21.419002926743396	14.549958189380103
7	16.483114836168387	24.629618423079464	43.24538432761921	15.641882413132935
8	38.75493750412605	25.04662434258302	20.662182569372618	15.536255583918315
9	38.28993189270074	26.038560669410032	21.12364665625069	14.547860781638535
10-11	14.584084044847392	48.96104391765508	20.939504571661203	15.515367465836322
12-13	14.553465330193868	25.475578609466805	32.44063992254033	27.530316137798998
14-15	15.093221178179258	36.886439468124905	20.666927524591248	27.35341182910459
16-17	27.127215237640716	25.1249290620233	32.164537939976626	15.583317760359353
18-19	15.48081181370068	25.593652350196948	32.15754145302907	26.7679943830733
20-21	27.26265594556907	37.306902736658095	20.684966526512575	14.745474791260257
22-23	26.654150979077485	25.730689222411335	32.32623308748947	15.288926711021714
24-25	15.111874353585812	37.17344049138481	20.70999315076029	27.00469200426908
26-27	15.6491797765906	31.425771062269174	37.74514809890201	15.179901062238226
28-29	27.763920066113577	25.562793198911333	31.64886635055153	15.024420384423562
30-31	23.183855685005884	28.391245563397767	32.277162390129405	16.147736361466944
32-33	17.59829303456739	29.81596929360225	33.59542058488082	18.990317086949542
34-35	19.009814177906794	29.847737117226004	33.46516899520939	17.677279709657814
36-37	19.16977719397483	28.76216200417106	34.89353142134048	17.17452938051363
38-39	20.801781836757467	28.84537383891839	33.44800508801464	16.904839236309495
40-41	16.985682867855186	32.46888865660063	33.373586441946124	17.17184203359805
42-43	16.895036834489627	29.049713494242376	35.253893892461996	18.801355778806002
44-45	16.968838093456664	30.940712829665195	33.037686313067326	19.05276276381081
46-47	18.969140650251077	29.129502814710488	34.5366971404522	17.364659394586237
48-49	17.180055675979006	29.30728931703399	34.50134719914615	19.01130780784085
50-51	19.084669931228255	31.262713419094982	32.58324984521673	17.069366804460028
52-53	19.133842320729457	29.820738050062957	33.47058617220343	17.574833457004157
54-55	17.89884412392268	32.0604707848724	30.150205608852005	19.890479482352916
56-57	18.47657082734481	32.78808529951794	30.145117145863274	18.59022672727398
58-59	22.29034080418055	31.59522639648155	27.63583668548963	18.47859611384827
60-61	20.426051287905697	33.479479026508415	27.28984987471507	18.80461981087082
62-63	18.697956289085166	33.29267401628952	27.342041132099798	20.66732856252552
64-65	19.999487644054977	33.08927080229611	27.971832114367473	18.939409439281434
66-67	19.863915541942752	31.34662197688002	29.93182366034662	18.857638820830612
68-69	21.38050489972053	31.32617933214421	28.626424206411023	18.666891561724235
70-71	17.64845553426249	34.606510176944134	28.600202950868493	19.144831337924888
72-73	17.647277269852992	31.43766726187222	30.234929110021564	20.680126358253226
74-75	17.987056023230046	33.0971417175483	27.987838266480193	20.927963992741468
76-77	19.923472497326987	31.36131857272219	29.109815900397333	19.605393029553486
78-79	18.80667075894584	31.795289752279555	28.361489245731047	21.036550243043557
80-81	20.688525159514867	33.55954519672454	26.714181989739398	19.03774765402119
82-83	20.50833449814979	32.260911321448596	28.15173114076192	19.079023039639694
84-85	19.29266614091489	33.75644488641063	26.285818129427437	20.665070843247054
86-87	19.301981321522888	33.930217044454224	27.648874202244716	19.118927431778168
88-89	22.760022886662878	32.09410902248917	26.2567721277857	18.889095963062246
90-91	20.748737793570665	33.80249508201118	26.121165627822833	19.327601496595317
92-93	19.201625544626395	33.84105239503224	26.019597523650983	20.93772453669038
94-95	20.497161918994596	34.07877303845396	25.832434415690926	19.591630626860518
96-97	20.749395087559066	32.36930186063553	27.17433634146136	19.70696671034404
98-99	22.14446744193363	32.51446684192438	25.564279472273814	19.776786243868173
100	19.026526611713358	35.33777787405438	25.533174683241374	20.10252083099089
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	166142.0
1	89591.0
2	9308.0
3	4671.0
4	3520.0
5	3135.5
6	3182.0
7	3655.5
8	3419.0
9	2996.5
10	3139.5
11	4549.5
12	35905.5
13	39192.5
14	10451.5
15	8588.0
16	9225.5
17	10198.0
18	11297.0
19	12505.5
20	13947.0
21	15602.5
22	17379.0
23	19379.0
24	21811.5
25	24495.0
26	27317.5
27	30205.5
28	33547.0
29	37296.0
30	40576.5
31	43166.5
32	46540.0
33	50383.5
34	54977.5
35	60243.5
36	64492.0
37	67930.5
38	70583.0
39	72726.5
40	74451.0
41	75510.0
42	74137.5
43	72274.5
44	71480.5
45	70659.0
46	72108.0
47	78552.0
48	83178.0
49	106681.0
50	192659.5
51	190265.0
52	97116.5
53	65956.5
54	62527.0
55	61638.5
56	56954.0
57	48746.0
58	40644.0
59	32686.0
60	26545.0
61	21419.0
62	16775.0
63	12827.5
64	9487.5
65	6972.0
66	5150.5
67	3783.0
68	2916.0
69	2298.0
70	1753.5
71	1501.0
72	1320.5
73	1013.5
74	684.0
75	463.0
76	331.5
77	251.5
78	170.5
79	110.0
80	53.0
81	28.0
82	23.0
83	13.5
84	9.5
85	8.5
86	5.5
87	4.0
88	3.5
89	3.0
90	1.0
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05693946262350637
2	0.07179323548181238
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	5.1575600202451426E-5
18-19	0.0
20-21	1.891105340756552E-4
22-23	6.876746693660189E-5
24-25	0.0
26-27	0.002028640274629756
28-29	8.080177365050722E-4
30-31	0.0054326298879915505
32-33	0.0011346632044539314
34-35	0.0020458321413639063
36-37	0.007461270162621306
38-39	0.011742044979424774
40-41	0.0030257685452104834
42-43	0.0071518165614065975
44-45	0.0014269249389344894
46-47	0.002063024008098057
48-49	0.0033008384129568913
50-51	0.00871627643421429
52-53	0.0032320709460202887
54-55	0.0013237737385295865
56-57	4.126048016196114E-4
58-59	8.595933367075237E-4
60-61	0.006223455757762471
62-63	0.0013581574719978876
64-65	0.007478462029355456
66-67	0.010487038707831789
68-69	0.011312248311071011
70-71	0.008905386968289946
72-73	0.003885361881918007
74-75	0.0017019948066808969
76-77	0.00400570494905706
78-79	0.015025691525647514
80-81	0.008166136698721476
82-83	0.00579365908940871
84-85	0.004658995884954779
86-87	0.006601676825913783
88-89	0.0037134432145765023
90-91	0.005191943753713443
92-93	0.009644637237858416
94-95	0.007736340030367714
96-97	0.009850939638668222
98-99	0.0021833670752371102
100	0.0022349426754395617
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2908352.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.12114796672488
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.19540503702845	38.26020694967976
2	12.033025509776385	11.340199510670962
3	3.3176597368165814	4.68995806085339
4	1.2007937308965493	2.263311162843677
5	0.6009294551831122	1.4158242887623402
6	0.3403496770465132	0.9622600495521466
7	0.23087398584400093	0.76153330740359
8	0.16030537314390006	0.6043018566219807
9	0.12223126924557097	0.5183709951853024
>10	0.6446208498925113	5.73190682442508
>50	0.10594947227879545	3.3633308249590756
>100	0.03923421885663497	3.340119859291198
>500	0.001842240169021528	0.6059925785083308
>1k	0.005968858147629569	7.7253289509332665
>5k	5.89516854086871E-4	1.7668649074499883
>10k+	2.2106882028257666E-4	16.650489872859907
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	239522	8.23566060779438	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	165813	5.701269997579385	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	74183	2.5506884998789694	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	9603	0.330186992496094	No Hit
GTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGT	7155	0.2460156129656933	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7106	0.24433081002574655	No Hit
AGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTC	5740	0.19736263010804744	No Hit
CCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGAT	5625	0.19340850075919283	No Hit
CACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCA	5345	0.18378105538806858	No Hit
TTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTG	5222	0.17955185617146754	No Hit
CCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATT	5088	0.17494443588671524	No Hit
CCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTC	4988	0.17150606253988512	No Hit
CTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCG	4979	0.17119660893867042	No Hit
ACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTC	4883	0.16789577052571353	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGTAACGAGTGGAATCACTGCTG	4809	0.16535137424905927	No Hit
TCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGT	4743	0.1630820478401514	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4722	0.16235998943731708	No Hit
TACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACT	4632	0.15926545342516998	No Hit
CGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAG	4474	0.15383282353717845	No Hit
TCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGA	4459	0.15331706753515395	No Hit
TCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCA	4438	0.1525950091323196	No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4322	0.1486064960499967	No Hit
ACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGA	4314	0.1483314261822503	No Hit
CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4313	0.14829704244878197	No Hit
ATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTA	4261	0.14650908830843035	No Hit
ACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAG	4243	0.1458901811060009	No Hit
TCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACT	4229	0.14540880883744473	No Hit
TGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCA	4170	0.14338016856281494	No Hit
GTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCAC	4012	0.13794753867482343	No Hit
CAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTT	3947	0.13571259599938384	No Hit
GATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGT	3934	0.13526560746429594	No Hit
CTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGC	3766	0.12948914024162136	No Hit
GAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGT	3723	0.1280106397024844	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC	3713	0.12766680236780142	No Hit
ATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCC	3601	0.12381582421935172	No Hit
GTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCAT	3462	0.11903648526725788	No Hit
TGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATG	3364	0.11566687938736439	No Hit
AGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCA	3280	0.11277864577602711	No Hit
CTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACG	3279	0.1127442620425588	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3220	0.11071562176792905	No Hit
CGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAG	3082	0.10597066654930351	No Hit
TTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTAC	3073	0.10566121294808882	No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3058	0.1051454569460643	No Hit
CTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGT	3029	0.10414832867548357	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0037822106815131045	0.0	0.0	3.4383733468300946E-5	0.0
2	0.003816594414981405	0.0	0.0	3.4383733468300946E-5	0.0
3	0.003816594414981405	0.0	0.0	3.4383733468300946E-5	0.0
4	0.003816594414981405	0.0	0.0	6.876746693660189E-5	0.0
5	0.003816594414981405	0.0	0.0	6.876746693660189E-5	0.0
6	0.003816594414981405	0.0	0.0	1.0315120040490284E-4	0.0
7	0.003816594414981405	0.0	0.0	1.0315120040490284E-4	0.0
8	0.003816594414981405	0.0	0.0	1.0315120040490284E-4	0.0
9	0.003816594414981405	0.0	0.0	1.0315120040490284E-4	0.0
10-11	0.003816594414981405	0.0	0.0	1.2034306713905332E-4	0.0
12-13	0.003936937482120459	0.0	0.0	1.3753493387320379E-4	0.0
14-15	0.00402289681579121	0.0	0.0	2.0630240080980568E-4	0.0
16-17	0.004091664282727813	0.0	0.0	2.5787800101225713E-4	0.0
18-19	0.004108856149461963	0.0	0.0	2.9226173448055806E-4	0.0
20-21	0.004297966683537619	0.0	0.0	3.26645467948859E-4	0.0
22-23	0.0045386528178157255	0.0	0.0	3.6102920141716E-4	0.0
24-25	0.004624612151486478	0.0	0.0	4.1260480161961136E-4	0.0
26-27	0.004951257619435337	0.0	0.0	4.2979666835376185E-4	0.0
28-29	0.0053122868208524965	0.0	0.0	4.6418040182206284E-4	0.0
30-31	0.005621740422067205	0.0	0.0	5.157560020245143E-4	0.0
32-33	0.005931194023281914	0.0	0.0	5.845234689611161E-4	0.0
34-35	0.006326606958167374	0.0	0.0	6.360990691635676E-4	0.0
36-37	0.007512845762823757	0.0	0.0	6.87674669366019E-4	0.0
38-39	0.009541486037453513	0.0	0.0	7.2205840283432E-4	0.0
40-41	0.012842324450410406	0.0	0.0	7.564421363026208E-4	0.0
42-43	0.01629788966397465	0.0	0.0	7.908258697709218E-4	0.0
44-45	0.02026921087956341	0.0	0.0	8.424014699733732E-4	0.0
46-47	0.02470471249697423	0.0	0.0	8.939770701758247E-4	0.0
48-49	0.03037802851924389	0.0	0.0	9.283608036441257E-4	0.0
50-51	0.037357926413308984	0.0	0.0	9.971282705807274E-4	0.0
52-53	0.04586790044671346	0.0	0.0	0.0010315120040490285	0.0
54-55	0.05687069515656977	0.0	0.0	0.0010315120040490285	0.0
56-57	0.07136343881345861	0.0	0.0	0.0011002794709856303	0.0
58-59	0.08632036287216953	0.0	0.0	0.0011690469379222323	0.0
60-61	0.10691621921968181	0.0	0.0	0.0012034306713905331	0.0
62-63	0.13248052505336355	0.0	0.0	0.0012378144048588342	0.0
64-65	0.1648528101137689	0.0	0.0	0.001306581871795436	0.0
66-67	0.2032078647976586	0.0	0.0	0.001375349338732038	0.0
68-69	0.2511903648526726	0.0	0.0	0.0014956924058710914	0.0
70-71	0.3164850747089761	0.0	0.0	0.0015988436062759942	0.0
72-73	0.39460491714895585	0.0	0.0	0.0016848029399467463	0.0
74-75	0.48506851990405564	0.0	0.0	0.0017879541403516494	0.0
76-77	0.5948901646018089	0.0	0.0	0.0017879541403516494	0.0
78-79	0.7246543747111767	0.0	0.0	0.0018223378738199503	0.0
80-81	0.8878223818849988	0.0	0.0	0.0018567216072882511	0.0
82-83	1.094176358295007	0.0	0.0	0.0018567216072882511	0.0
84-85	1.3450056939462622	0.0	0.0	0.001891105340756552	0.0
86-87	1.6361155733556325	0.0	0.0	0.001925489074224853	0.0
88	1.8756670444292851	0.0	0.0	0.001959872807693154	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	2795	0.0	89.06165	1
AACGTGT	1470	0.0	71.7047	1
ATCGAGT	985	0.0	68.3151	1
ACGTGTG	1730	0.0	68.235886	2
CGTGTGG	1995	0.0	65.72194	3
TCGAGTG	1200	0.0	64.27585	2
GTGGAAT	123030	0.0	50.829773	6
ATCTGCG	895	0.0	50.41468	94
TGGAATC	124855	0.0	50.402973	7
GGAATCA	125855	0.0	50.00622	8
AGTGGAA	122290	0.0	49.938377	5
GAATCAC	126335	0.0	49.845985	9
ACGAGTG	123415	0.0	48.431583	2
AACGAGT	118755	0.0	48.060684	1
CGAGTGG	126185	0.0	47.976067	3
GAGTGGA	128690	0.0	47.52788	4
GTGTGGA	3050	0.0	46.994724	4
CGATTGG	755	0.0	44.816162	3
ACGATTG	740	0.0	44.488884	2
AGTACTT	38005	0.0	39.557953	20-21
>>END_MODULE