FastQCFastQC Report
Thu 26 May 2016
SRR2132681_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132681_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2130591
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1622337.614460025410789No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1131385.310169807344535No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG214121.0049793695739822No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT62460.29315809557066563No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA47880.22472637873716728No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC31500.14784630180076794No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT31020.1455934057733277No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT28870.13550230898375146No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24330.11419366739087887No Hit
CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT22490.10555756595235781No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT16400.082.0384451
CGTGTGG11900.067.1365053
GTGGAAT567050.064.5282446
TGGAATC573750.064.176067
GGAATCA579300.063.6423458
AACGAGT525700.063.633811
GAATCAC579750.063.5686269
ACGAGTG550050.063.494062
AGTGGAA563900.063.3635755
CGAGTGG562100.062.8474583
AACGTGT10050.062.255941
GAGTGGA578600.061.8430944
ACGTGTG11700.059.8855742
ATCGAGT6350.055.562631
TCGAGTG7400.054.64982
ATCTGCG8000.051.7011794
CGATTGG3550.045.0098273
ACGATTG3200.042.6157232
AGTACTT247000.041.4788320-21
GAGTACT244600.040.93474220-21