##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2132681_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2130591 Sequences flagged as poor quality 0 Sequence length 100 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.261379589043603 31.0 28.0 34.0 16.0 34.0 2 30.366356564915556 33.0 30.0 34.0 23.0 34.0 3 30.79412566747912 33.0 31.0 34.0 25.0 34.0 4 35.19064428602205 37.0 35.0 37.0 32.0 37.0 5 34.65084429625395 37.0 35.0 37.0 30.0 37.0 6 35.15262948167903 37.0 35.0 37.0 32.0 37.0 7 35.528659418912405 37.0 35.0 37.0 33.0 37.0 8 35.715685460043716 37.0 35.0 37.0 35.0 37.0 9 37.60590934627998 39.0 38.0 39.0 35.0 39.0 10-11 37.674710444191305 39.0 39.0 39.0 35.0 39.0 12-13 37.64081351136844 39.0 39.0 39.0 35.0 39.0 14-15 39.04407368659682 41.0 39.0 41.0 36.0 41.0 16-17 38.9272636090174 41.0 39.0 41.0 36.0 41.0 18-19 38.678901534832356 41.0 38.5 41.0 35.5 41.0 20-21 38.477052376547164 40.5 39.0 41.0 35.0 41.0 22-23 38.360079668035766 40.0 38.0 41.0 35.0 41.0 24-25 38.26019634927586 40.0 38.0 41.0 34.5 41.0 26-27 38.19844282642703 40.0 38.0 41.0 34.5 41.0 28-29 38.024116078590396 40.0 38.0 41.0 34.0 41.0 30-31 37.88268583693444 40.0 38.0 41.0 34.0 41.0 32-33 37.74087448036718 40.0 38.0 41.0 34.0 41.0 34-35 37.649757508597375 40.0 38.0 41.0 33.0 41.0 36-37 37.47908491118192 40.0 37.5 41.0 33.0 41.0 38-39 37.02611575849143 40.0 36.0 41.0 33.0 41.0 40-41 36.832040264884256 40.0 35.0 41.0 33.0 41.0 42-43 36.69697562788916 40.0 35.0 41.0 33.0 41.0 44-45 36.55489110767857 40.0 35.0 41.0 33.0 41.0 46-47 36.4122112127574 39.0 35.0 41.0 32.0 41.0 48-49 36.247026059905444 39.0 35.0 41.0 31.5 41.0 50-51 35.98023646959928 39.0 35.0 41.0 31.0 41.0 52-53 35.486215327108766 39.0 35.0 41.0 29.0 41.0 54-55 34.66852624459598 38.5 35.0 41.0 24.0 41.0 56-57 34.05277056929275 38.0 35.0 41.0 17.0 41.0 58-59 33.754016843213925 38.0 35.0 40.0 11.0 41.0 60-61 33.50732777900592 37.5 34.0 40.0 7.5 41.0 62-63 33.221817561418405 37.0 34.0 40.0 7.0 41.0 64-65 32.953960192265896 36.0 34.0 40.0 3.5 41.0 66-67 32.634452130887624 36.0 34.0 39.5 2.0 41.0 68-69 32.36078228998433 35.0 33.5 39.0 2.0 41.0 70-71 32.04243564344353 35.0 33.0 39.0 2.0 41.0 72-73 31.71864778364313 35.0 33.0 38.0 2.0 40.0 74-75 31.390937303311617 35.0 33.0 37.0 2.0 40.0 76-77 31.03996050861005 35.0 33.0 37.0 2.0 39.0 78-79 30.916903807441223 35.0 33.0 36.0 2.0 39.0 80-81 30.77279637433933 35.0 33.0 36.0 2.0 39.0 82-83 30.47062739868891 35.0 33.0 36.0 2.0 37.0 84-85 30.20448293454727 35.0 33.0 35.0 2.0 37.0 86-87 30.0344664930998 35.0 33.0 35.0 2.0 37.0 88-89 29.838506076483004 35.0 33.0 35.0 2.0 36.0 90-91 29.648225069945383 35.0 33.0 35.0 2.0 36.0 92-93 29.495613658369905 35.0 32.5 35.0 2.0 36.0 94-95 29.313439088027685 35.0 32.0 35.0 2.0 35.5 96-97 29.11435653299953 35.0 32.0 35.0 2.0 35.0 98-99 28.96725274818114 35.0 32.0 35.0 2.0 35.0 100 28.871019825015686 35.0 32.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 2.7536165770937977 1101 2 2.217332643663653 1101 3 2.054768798556747 1101 4 1.0724779775210749 1101 5 1.6276102682624298 1101 6 1.0585632837399004 1101 7 0.5523964964736763 1101 8 0.43188320598124363 1101 9 0.37786248769238284 1101 10-11 0.3708604072007944 1101 12-13 0.33678165094253387 1101 14-15 0.5065770294502627 1101 16-17 0.4978541018781044 1101 18-19 0.531985315010779 1101 20-21 0.5667139790500215 1101 22-23 0.5567879879835118 1101 24-25 0.6288537458601624 1101 26-27 0.6133930401732925 1101 28-29 0.7253875650757422 1101 30-31 0.7474437190406746 1101 32-33 0.6563089838654506 1101 34-35 0.6373673423256747 1101 36-37 0.5708552428730158 1101 38-39 0.5309123014853228 1101 40-41 0.5458477948674911 1101 42-43 0.5055622594723843 1101 44-45 0.5487094873866241 1101 46-47 0.4961686125175504 1101 48-49 0.3567192066037421 1101 50-51 0.3829047255079914 1101 52-53 0.3834382275759367 1101 54-55 0.29565817224197133 1101 56-57 0.33485844320043867 1101 58-59 0.23079563754318144 1101 60-61 0.2517237718439773 1101 62-63 0.2902733729801028 1101 64-65 0.24851623266506806 1101 66-67 0.1912934272710345 1101 68-69 0.2376990840792388 1101 70-71 0.266084243635504 1101 72-73 0.2953715414412237 1101 74-75 0.3453248458960587 1101 76-77 0.32337748055726223 1101 78-79 0.29266960736898895 1101 80-81 0.35865725599791176 1101 82-83 0.4154279225250228 1101 84-85 0.4103689751643991 1101 86-87 0.40287149431716074 1101 88-89 0.37246826299513813 1101 90-91 0.27701040647842845 1101 92-93 0.3374805022882086 1101 94-95 0.40042947706604437 1101 96-97 0.4749173496723813 1101 98-99 0.4103790844980999 1101 100 0.47633289747344065 1102 1 2.749542905113959 1102 2 2.22576006285459 1102 3 2.0795358106108246 1102 4 1.0560960927173468 1102 5 1.5444463123768912 1102 6 0.9568282302633406 1102 7 0.43209924897253416 1102 8 0.38263380566547767 1102 9 0.35423755158998915 1102 10-11 0.33471986324320824 1102 12-13 0.28355801793515667 1102 14-15 0.4613720258007703 1102 16-17 0.4793542328185936 1102 18-19 0.4503898384870695 1102 20-21 0.5295611866883476 1102 22-23 0.4650216384709154 1102 24-25 0.5417467197655554 1102 26-27 0.5134017792173111 1102 28-29 0.6203005607793912 1102 30-31 0.5813694621352354 1102 32-33 0.5673140145740518 1102 34-35 0.5629152842566185 1102 36-37 0.5660820602602854 1102 38-39 0.620585877384066 1102 40-41 0.5946418067932342 1102 42-43 0.5920273093933233 1102 44-45 0.6003285912658853 1102 46-47 0.5823350058511778 1102 48-49 0.6138501890987484 1102 50-51 0.5952216870536873 1102 52-53 0.47071805060400607 1102 54-55 0.48948755552095236 1102 56-57 0.5453948850139909 1102 58-59 0.5581942438253478 1102 60-61 0.4630660348092661 1102 62-63 0.3903717670670588 1102 64-65 0.3563397287510739 1102 66-67 0.39804478879086247 1102 68-69 0.36172508713858065 1102 70-71 0.39442862158492886 1102 72-73 0.4257681430660405 1102 74-75 0.3342882946267096 1102 76-77 0.3566185103592403 1102 78-79 0.4722432678322548 1102 80-81 0.5455977950778923 1102 82-83 0.4970411094037175 1102 84-85 0.5043214889605707 1102 86-87 0.5337629697136066 1102 88-89 0.5003869149796891 1102 90-91 0.47021794338613176 1102 92-93 0.40422608810320426 1102 94-95 0.4129112610465526 1102 96-97 0.4173206138746792 1102 98-99 0.34277909502753445 1102 100 0.3201511803277448 1103 1 2.5892308870164236 1103 2 2.163733282391405 1103 3 1.9531123893985516 1103 4 1.043615156566581 1103 5 1.5840893746022218 1103 6 0.919791686330818 1103 7 0.4432514078183871 1103 8 0.3679508791562327 1103 9 0.32839600227790555 1103 10-11 0.23775187391588304 1103 12-13 0.18378930217483003 1103 14-15 0.374828469536169 1103 16-17 0.4030203518819846 1103 18-19 0.40750071213619776 1103 20-21 0.47444673991817155 1103 22-23 0.4311454388762641 1103 24-25 0.5395308849619127 1103 26-27 0.495110951707332 1103 28-29 0.5210150499222621 1103 30-31 0.5805836572162946 1103 32-33 0.6111248336558006 1103 34-35 0.5461947159069211 1103 36-37 0.6351232470505082 1103 38-39 0.6578178354188964 1103 40-41 0.6071155554097558 1103 42-43 0.6304848626138764 1103 44-45 0.6055335237860291 1103 46-47 0.6717638506127841 1103 48-49 0.6286926402775492 1103 50-51 0.6759884917981864 1103 52-53 0.5973873916122727 1103 54-55 0.5043412462667334 1103 56-57 0.5313500571829266 1103 58-59 0.5345353412392981 1103 60-61 0.6011176322363383 1103 62-63 0.5401118870535342 1103 64-65 0.575057264250745 1103 66-67 0.5611280113686377 1103 68-69 0.5398346942380527 1103 70-71 0.49029479567877843 1103 72-73 0.5334217768518137 1103 74-75 0.5116218892196756 1103 76-77 0.5049577847443771 1103 78-79 0.5487359498611433 1103 80-81 0.563956011751273 1103 82-83 0.6091814529080395 1103 84-85 0.6332909374055937 1103 86-87 0.650139424007449 1103 88-89 0.6008633857903902 1103 90-91 0.6471647618072147 1103 92-93 0.5894231828343237 1103 94-95 0.6275711199123251 1103 96-97 0.5990605528703625 1103 98-99 0.6244512300088481 1103 100 0.6620452046214176 1104 1 1.6602755604696995 1104 2 1.730190776334105 1104 3 1.7994873625912824 1104 4 0.8886356023352917 1104 5 1.5006580331438144 1104 6 0.9818645762377471 1104 7 0.4293243676903842 1104 8 0.2684954469014258 1104 9 0.20068504620532224 1104 10-11 0.19606652771810218 1104 12-13 0.17929095738091405 1104 14-15 0.24883569761162505 1104 16-17 0.3148352361126143 1104 18-19 0.29743131363801467 1104 20-21 0.3589015770896893 1104 22-23 0.35159635039936177 1104 24-25 0.4384822740726122 1104 26-27 0.3554998034589687 1104 28-29 0.4435309289496203 1104 30-31 0.4805397959661448 1104 32-33 0.3745818435467356 1104 34-35 0.28950045676850067 1104 36-37 0.33047259515863203 1104 38-39 0.3955816018055529 1104 40-41 0.3776243972446025 1104 42-43 0.3390633588517389 1104 44-45 0.38831500768418437 1104 46-47 0.4654877372937207 1104 48-49 0.4830819878685446 1104 50-51 0.4406464101702525 1104 52-53 0.4272759912615385 1104 54-55 0.48575630107522727 1104 56-57 0.42763150914051096 1104 58-59 0.4563764281465481 1104 60-61 0.39081862285036806 1104 62-63 0.4923902480002553 1104 64-65 0.49989615448316727 1104 66-67 0.5156658350901395 1104 68-69 0.5725638944613252 1104 70-71 0.5690947541560405 1104 72-73 0.4788634769420135 1104 74-75 0.535640771801571 1104 76-77 0.5018411548854154 1104 78-79 0.5321409688657823 1104 80-81 0.4638438014788804 1104 82-83 0.5216565373604354 1104 84-85 0.4749396565125963 1104 86-87 0.42209766437090224 1104 88-89 0.3988735356677253 1104 90-91 0.3559485427648532 1104 92-93 0.3937659789424437 1104 94-95 0.48990330832824114 1104 96-97 0.5357059524393151 1104 98-99 0.5471000046316057 1104 100 0.520243037681734 1105 1 2.1874787682584724 1105 2 2.0587696551494687 1105 3 1.9651888617785467 1105 4 1.0320915172601133 1105 5 1.6422152368874379 1105 6 1.141442286869541 1105 7 0.6338089741284989 1105 8 0.42850980421879115 1105 9 0.3445308144117192 1105 10-11 0.2732401363850414 1105 12-13 0.25115897392413444 1105 14-15 0.359709548833564 1105 16-17 0.3179275461029576 1105 18-19 0.26442370521402125 1105 20-21 0.34711129081315306 1105 22-23 0.37130265848053057 1105 24-25 0.44661928002143014 1105 26-27 0.4056260905987017 1105 28-29 0.4031182643423534 1105 30-31 0.4162802492859612 1105 32-33 0.46803345531341733 1105 34-35 0.37582451558799335 1105 36-37 0.3698475472183347 1105 38-39 0.43082685245855146 1105 40-41 0.3204779526074475 1105 42-43 0.4260795930433616 1105 44-45 0.46353567987234356 1105 46-47 0.5269309174395147 1105 48-49 0.43090848930371806 1105 50-51 0.39440626935697054 1105 52-53 0.43681831323465303 1105 54-55 0.5351755949315731 1105 56-57 0.5745206836424686 1105 58-59 0.5271097963944911 1105 60-61 0.598091795035792 1105 62-63 0.6670609875892666 1105 64-65 0.6361107901387086 1105 66-67 0.5719924317636611 1105 68-69 0.5546913831822877 1105 70-71 0.6288304338146276 1105 72-73 0.621474908552262 1105 74-75 0.672492394138061 1105 76-77 0.6149814583929398 1105 78-79 0.6873628558474181 1105 80-81 0.6521248613806776 1105 82-83 0.6819954665732055 1105 84-85 0.7300132084035447 1105 86-87 0.6196158339744215 1105 88-89 0.5892257083313481 1105 90-91 0.6214722086665532 1105 92-93 0.7086451253426738 1105 94-95 0.6917988632484651 1105 96-97 0.6313342832580418 1105 98-99 0.5837121731920938 1105 100 0.5990099158790549 1106 1 1.6551596740035137 1106 2 1.0218177857565856 1106 3 1.5486670796295776 1106 4 0.8192490129295891 1106 5 1.5420824473352113 1106 6 1.0706492794798024 1106 7 0.6449321900914313 1106 8 0.4132569791041618 1106 9 0.19452230819274519 1106 10-11 0.1358658566998514 1106 12-13 0.13254486655571185 1106 14-15 0.1797918954395641 1106 16-17 0.23550450869943518 1106 18-19 0.26023332464436066 1106 20-21 0.3036795688803835 1106 22-23 0.25905317819627527 1106 24-25 0.3644953839168892 1106 26-27 0.33746662995704924 1106 28-29 0.4093112099463312 1106 30-31 0.3483667215861175 1106 32-33 0.3500492174439529 1106 34-35 0.3982251894656059 1106 36-37 0.35884947766735564 1106 38-39 0.3976306211050513 1106 40-41 0.3871818799536584 1106 42-43 0.3049821324166615 1106 44-45 0.3533522053559466 1106 46-47 0.3719256749037214 1106 48-49 0.29764170793953326 1106 50-51 0.2490946018497695 1106 52-53 0.4164090377545264 1106 54-55 0.4335424275822106 1106 56-57 0.4676228696695546 1106 58-59 0.5315987844000105 1106 60-61 0.5428343377688591 1106 62-63 0.6022588586143485 1106 64-65 0.5622886879210114 1106 66-67 0.5149459547085584 1106 68-69 0.5226850437357484 1106 70-71 0.5682102325767104 1106 72-73 0.5858582308109028 1106 74-75 0.6364895554099235 1106 76-77 0.43101747849323857 1106 78-79 0.47171089687230605 1106 80-81 0.4525266111704269 1106 82-83 0.4876809930814687 1106 84-85 0.5454310595048533 1106 86-87 0.5532029954918585 1106 88-89 0.5499252861168067 1106 90-91 0.6306878427411142 1106 92-93 0.6586592549197903 1106 94-95 0.6528877162222102 1106 96-97 0.6529588091431044 1106 98-99 0.7401939476391952 1106 100 0.7358609207340052 1107 1 1.601971547618735 1107 2 1.0729733247829998 1107 3 1.4301149505572504 1107 4 0.7546917999767473 1107 5 1.4750778493880574 1107 6 1.0920435924805432 1107 7 0.6096200086014605 1107 8 0.5478741179916398 1107 9 0.5324585298765996 1107 10-11 0.2846058069136177 1107 12-13 0.13947187304827935 1107 14-15 0.20339992439207322 1107 16-17 0.2559769078594911 1107 18-19 0.21534058084001373 1107 20-21 0.21886573223468986 1107 22-23 0.2059134258644022 1107 24-25 0.25972227269779324 1107 26-27 0.2176603717667973 1107 28-29 0.1922340289914075 1107 30-31 0.10255552862847139 1107 32-33 0.10274899833536466 1107 34-35 0.1137358931567718 1107 36-37 0.13559923250782902 1107 38-39 0.13559591984882502 1107 40-41 0.22219272135517798 1107 42-43 0.1962039741567594 1107 44-45 0.20586397213599383 1107 46-47 0.2269167105733345 1107 48-49 0.2846180796487374 1107 50-51 0.14811142005630273 1107 52-53 0.14925269527149254 1107 54-55 0.17677383185947804 1107 56-57 0.13114729878556375 1107 58-59 0.04292221603690649 1107 60-61 0.007025960417912813 1107 62-63 0.07384245757371133 1107 64-65 0.06130133973132956 1107 66-67 0.08364596891816944 1107 68-69 0.07181900346116521 1107 70-71 0.008440384303703752 1107 72-73 -0.0035899102746803635 1107 74-75 0.049678830842921684 1107 76-77 0.15495833671823078 1107 78-79 0.08280653627817003 1107 80-81 0.12238207669283696 1107 82-83 0.06413725820110017 1107 84-85 0.0420128603353227 1107 86-87 0.03133827442989201 1107 88-89 0.03040484270699295 1107 90-91 0.035367056546533604 1107 92-93 0.12689153633310468 1107 94-95 0.13409305903329027 1107 96-97 0.10469694193097823 1107 98-99 0.09518226418706988 1107 100 0.13040495910344418 1108 1 0.40154901858082326 1108 2 0.17740768814044117 1108 3 0.0017713169828503794 1108 4 0.13656512892700334 1108 5 1.100772348621021 1108 6 0.7792471866072077 1108 7 0.42407672567355803 1108 8 0.4196913435011709 1108 9 0.3842555932121243 1108 10-11 0.22663758425308345 1108 12-13 0.11192376873094645 1108 14-15 0.1264623833006766 1108 16-17 0.1728756586813205 1108 18-19 0.17729258609971055 1108 20-21 0.21964394832060208 1108 22-23 0.1662182691692493 1108 24-25 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-0.7900835526861201 2215 78-79 -0.785045510484192 2215 80-81 -0.7707308480672808 2215 82-83 -0.6362549679289486 2215 84-85 -0.6453526095654318 2215 86-87 -0.7097980211198589 2215 88-89 -0.7170415747012662 2215 90-91 -0.730368527627963 2215 92-93 -0.8065564776865983 2215 94-95 -0.7505840600701141 2215 96-97 -0.7491066991050559 2215 98-99 -0.7831614419255963 2215 100 -0.7633828024022868 2216 1 -5.937821351789548 2216 2 -4.9192914203973785 2216 3 -5.9533170021054715 2216 4 -3.179718392808134 2216 5 -4.285644616731432 2216 6 -2.126027604312739 2216 7 -1.4196317570026622 2216 8 -1.0088399495785083 2216 9 -0.7167875094438756 2216 10-11 -1.1283761552929121 2216 12-13 -0.8577103182095982 2216 14-15 -1.709076216092754 2216 16-17 -1.188583122680683 2216 18-19 -1.6638014309943117 2216 20-21 -1.1353072969693585 2216 22-23 -0.9048185840611538 2216 24-25 -1.5672294356434975 2216 26-27 -1.3919144516727897 2216 28-29 -2.1753480477407905 2216 30-31 -2.202438267897584 2216 32-33 -1.3183380411759913 2216 34-35 -1.3073535323061023 2216 36-37 -1.3498846384599048 2216 38-39 -1.192899499124266 2216 40-41 -1.226215848982676 2216 42-43 -1.579285145904322 2216 44-45 -1.521934748993985 2216 46-47 -1.8661807316866401 2216 48-49 -1.729012861370542 2216 50-51 -1.5273299809744216 2216 52-53 -1.30998332305262 2216 54-55 -1.1712386705264777 2216 56-57 -1.0512610806761629 2216 58-59 -0.9112054800883058 2216 60-61 -0.9345754902406043 2216 62-63 -0.9815736523709333 2216 64-65 -1.0119425999518192 2216 66-67 -1.2792405552276627 2216 68-69 -1.2391611454222087 2216 70-71 -1.1551905567155742 2216 72-73 -1.202077217012608 2216 74-75 -1.1203512321461986 2216 76-77 -1.0926485539097506 2216 78-79 -1.0705385677493169 2216 80-81 -1.089440313076203 2216 82-83 -1.0921401802413513 2216 84-85 -1.0625991839479347 2216 86-87 -1.0576171559957608 2216 88-89 -1.1089108663977996 2216 90-91 -1.2007347758642304 2216 92-93 -1.1900487194408989 2216 94-95 -1.2622635929995454 2216 96-97 -1.2745428939155587 2216 98-99 -1.258907256713865 2216 100 -1.3030556249774854 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5512.0 3 5327.0 4 5720.0 5 7653.0 6 6309.0 7 4433.0 8 3922.0 9 3954.0 10 3906.0 11 6687.0 12 4509.0 13 5425.0 14 5071.0 15 5329.0 16 6238.0 17 7686.0 18 10649.0 19 17093.0 20 35679.0 21 47118.0 22 21900.0 23 13826.0 24 13880.0 25 14920.0 26 15769.0 27 17743.0 28 19903.0 29 22789.0 30 26780.0 31 32870.0 32 43066.0 33 60970.0 34 89114.0 35 223199.0 36 253297.0 37 402003.0 38 490086.0 39 168912.0 40 1344.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 14.442642651950516 44.436382557959874 24.863186655527176 16.257788134562436 2 16.834337952043192 43.19818182330547 23.817964404842083 16.14951581980926 3 17.540062827637964 26.266327042590532 21.127705880668792 35.065904249102715 4 33.572515794913244 27.330585738886537 21.935181365170507 17.161717101029712 5 16.65251566349431 42.39363631968782 23.240265259733096 17.713582757084772 6 35.95959994198793 25.410461228832755 22.89064395747471 15.739294871704612 7 17.5075835765757 25.39079532392655 40.19518527957736 16.906435819920386 8 34.94992703902344 25.89680515875642 22.479537367800763 16.67373043441937 9 34.7094303880942 26.74506744842159 22.608187118034387 15.937315045449832 10-11 16.03670061499368 45.07275211431946 22.458533805878275 16.43201346480859 12-13 16.0036346722576 26.592762289899845 31.4581963408275 25.945406697015056 14-15 16.302190331227344 35.626007056258096 22.32929736397084 25.74250524854371 16-17 25.809271520692167 26.381531116512853 31.250020534227858 16.559176828567125 18-19 16.64756398576733 26.61822470854331 31.275547488936166 25.45866381675319 20-21 25.986231018133036 35.845279186101855 22.080896992658356 16.08759280310675 22-23 25.615463322366104 26.71424347246537 31.259252132038544 16.41104107312998 24-25 16.531328631351585 35.74165571900003 22.101308979527275 25.625706670121108 26-27 16.909605459827503 30.595057082852335 36.16222808874824 16.333109368571915 28-29 25.17639620890718 26.607185015486444 31.902380251697547 16.314038523908827 30-31 22.57483926763069 28.19924594546071 32.32840448114453 16.89751030576407 32-33 18.864758130226306 29.224601139226724 33.37976070231037 18.530880028236602 34-35 19.100310388316295 29.23059556726246 33.48039972645651 18.18869431796474 36-37 19.169065436243578 28.939898353972726 33.968525170358596 17.922511039425093 38-39 19.94840670313857 28.742503164437384 33.47698967594236 17.83210045648168 40-41 18.35532054755685 30.282417167464164 33.46237077969518 17.899891505283808 42-43 18.436915324151563 28.853263201176837 34.172997868060044 18.53682360661155 44-45 18.349947103158698 29.61392346244794 33.30670499145295 18.729424442940406 46-47 19.136634425496613 28.933858860885003 33.849919563306784 18.079587150311596 48-49 18.463237944681843 29.056372364952647 33.825132174801176 18.655257515564333 50-51 19.157019781317448 29.81302989024565 32.884198885276625 18.14575144316028 52-53 19.435381121497915 29.279882583455 32.993504681571686 18.291231613475397 54-55 19.14494163058865 30.48121439916492 31.100767494865217 19.273076475381213 56-57 19.477376929609996 31.179568751130855 30.392482420862493 18.950571898396657 58-59 20.93120292502854 31.12172638639055 28.962452668349307 18.984618020231604 60-61 20.40222507086534 31.979268219985553 28.59824384437271 19.02026286477639 62-63 19.517516634707157 31.891983353676178 28.58858032897916 20.001919682637503 64-65 19.98161863071003 31.768648218330863 29.1785696515895 19.071163499369607 66-67 19.866850858034173 30.94694265330387 30.092288136508145 19.093918352153803 68-69 20.43651971823416 30.992224049516736 29.623646437522837 18.947609794726265 70-71 18.99304940286825 32.32693036611798 29.642307696821124 19.037712534192643 72-73 18.991785965735744 31.08878197606196 30.221943205820228 19.69748885238207 74-75 19.14061995801549 31.84474882629934 29.268908578663467 19.745722637021704 76-77 20.038911282042253 31.04521852392938 29.6350952540205 19.28077494000786 78-79 19.511628944088592 31.51337052780314 29.061046968775894 19.913953559332377 80-81 20.21380810780998 32.30612345481983 28.25505828676572 19.22501015060446 82-83 20.133889858283275 32.0039239937432 28.67045375871428 19.191732389259244 84-85 19.753161171828484 32.52842819254358 27.9630199396902 19.755390695937734 86-87 19.61486413858526 32.64560469348394 28.44274641471281 19.296784753217995 88-89 21.169840864543357 32.021363878729616 27.761308079935716 19.047487176791307 90-91 20.390737670195254 32.72268984478686 27.579780399280434 19.306792085737452 92-93 19.570396149379587 32.82201864663122 27.675371932792515 19.932213271196673 94-95 20.071005061698173 32.99585549552281 27.452607160635402 19.480532282143614 96-97 20.123236635086908 32.28296171609385 28.09259223619526 19.501209412623986 98-99 20.82786135035554 32.31294736101007 27.261129755227525 19.598061533406867 100 19.624385953219328 33.58715028786156 27.264381109372376 19.524082649546735 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 114909.0 1 62136.5 2 6864.5 3 3562.0 4 2608.5 5 2317.0 6 2314.5 7 2676.5 8 2540.0 9 2257.5 10 2338.5 11 3415.0 12 28911.0 13 31352.0 14 8063.5 15 7022.0 16 7726.0 17 8481.0 18 9074.5 19 9773.0 20 10580.5 21 11654.0 22 13058.0 23 14775.0 24 16586.0 25 18136.0 26 20068.5 27 22408.5 28 24455.0 29 26525.0 30 29234.5 31 31839.0 32 34026.0 33 36839.0 34 40436.5 35 43024.0 36 45701.5 37 49402.5 38 51854.5 39 52881.5 40 53625.0 41 55076.0 42 56932.0 43 58489.0 44 60495.0 45 62163.0 46 63028.5 47 65246.0 48 66030.0 49 70208.5 50 91941.5 51 89461.5 52 61611.0 53 52032.0 54 50524.0 55 49953.5 56 46852.5 57 41740.5 58 36656.0 59 30232.0 60 24938.0 61 20574.5 62 16085.5 63 12279.5 64 9759.5 65 7839.0 66 5959.0 67 4437.0 68 3282.0 69 2449.5 70 1723.0 71 1292.5 72 1028.0 73 668.0 74 450.5 75 348.5 76 274.5 77 205.5 78 130.5 79 90.0 80 54.5 81 20.0 82 10.5 83 8.5 84 6.0 85 2.5 86 1.0 87 0.5 88 0.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.057730460703157016 2 0.06688285081463312 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 9.38706678100114E-5 18-19 0.0 20-21 2.3467666952502848E-4 22-23 4.69353339050057E-5 24-25 0.0 26-27 0.0022294283604877706 28-29 8.448360102901026E-4 30-31 0.005092483728693118 32-33 0.0010795126798151312 34-35 0.0015958013527701937 36-37 0.0074392504239434035 38-39 0.01293068449082907 40-41 0.0034028117081129133 42-43 0.0064301407449857805 44-45 0.0013611246832451653 46-47 0.001572333685817691 48-49 0.0037078913784954503 50-51 0.009246260779286123 52-53 0.002651846365632822 54-55 0.0010795126798151312 56-57 5.397563399075656E-4 58-59 6.33627007717577E-4 60-61 0.00666481741451081 62-63 0.001431527684102674 64-65 0.008119812765565986 66-67 0.009293196113191128 68-69 0.011287947804153872 70-71 0.009316663780143631 72-73 0.004271115385355519 74-75 0.002041687024867748 76-77 0.00485780705916809 78-79 0.016239625531131972 80-81 0.008260618767281003 82-83 0.005092483728693118 84-85 0.004294583052308022 86-87 0.006946429417940844 88-89 0.0037078913784954503 90-91 0.006265867076318261 92-93 0.010935932799866328 94-95 0.008424892435948524 96-97 0.010231902791291242 98-99 0.0019243486901052336 100 0.0024875726969653023 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2130591.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.366477156307674 #Duplication Level Percentage of deduplicated Percentage of total 1 85.01254416942429 50.46895261433724 2 10.653145910870315 12.6487948672099 3 2.3089764394927093 4.112273911487897 4 0.7112227448292646 1.6889115533581194 5 0.3426645004917715 1.0171392120361165 6 0.20582166661052495 0.733134436146414 7 0.1302151958015304 0.5411292212868979 8 0.08772387305550174 0.4166285844649822 9 0.06486815202709907 0.34658942989398034 >10 0.40846836872702974 5.648538986310303 >50 0.05107030639505342 1.9684391377847075 >100 0.016628821056667346 1.8890578617372928 >500 0.003324925609120916 1.369786499509724 >1k 0.0030082660272998754 2.909045657951447 >5k 7.916489545525987E-5 0.2935458202133306 >10k+ 2.3749468636577963E-4 13.948032206271677 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 162233 7.614460025410789 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 113138 5.310169807344535 No Hit AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG 21412 1.0049793695739822 No Hit TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 6246 0.29315809557066563 No Hit TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4788 0.22472637873716728 No Hit AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC 3150 0.14784630180076794 No Hit ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 3102 0.1455934057733277 No Hit AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 2887 0.13550230898375146 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2433 0.11419366739087887 No Hit CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 2249 0.10555756595235781 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.004458856720975541 0.0 0.0 0.0 0.0 2 0.004458856720975541 0.0 0.0 0.0 0.0 3 0.004458856720975541 0.0 0.0 0.0 0.0 4 0.004458856720975541 0.0 0.0 0.0 0.0 5 0.004458856720975541 0.0 0.0 0.0 0.0 6 0.004458856720975541 0.0 0.0 0.0 0.0 7 0.004458856720975541 0.0 0.0 0.0 0.0 8 0.004458856720975541 0.0 0.0 0.0 0.0 9 0.004458856720975541 0.0 0.0 0.0 0.0 10-11 0.004458856720975541 0.0 0.0 0.0 0.0 12-13 0.004552727388785553 0.0 0.0 0.0 0.0 14-15 0.004670065723548067 0.0 0.0 0.0 0.0 16-17 0.004904742393073096 0.0 0.0 0.0 0.0 18-19 0.004998613060883107 0.0 0.0 0.0 0.0 20-21 0.00502208072783561 0.0 0.0 0.0 0.0 22-23 0.005069016061740616 0.0 0.0 0.0 0.0 24-25 0.005139419062598124 0.0 0.0 0.0 0.0 26-27 0.005162886729550627 0.0 0.0 0.0 0.0 28-29 0.005162886729550627 0.0 0.0 0.0 0.0 30-31 0.005256757397360638 0.0 0.0 0.0 0.0 32-33 0.005374095732123153 0.0 0.0 0.0 0.0 34-35 0.005467966399933164 0.0 0.0 0.0 0.0 36-37 0.00567917540250569 0.0 0.0 0.0 0.0 38-39 0.00617199640850825 0.0 0.0 0.0 0.0 40-41 0.006688285081463312 0.0 0.0 0.0 0.0 42-43 0.007274976755275884 0.0 0.0 0.0 0.0 44-45 0.008260618767281003 0.0 0.0 0.0 0.0 46-47 0.009175857778428614 0.0 0.0 0.0 0.0 48-49 0.009997226121766215 0.0 0.0 0.0 0.0 50-51 0.010842062132056317 0.0 0.0 0.0 0.0 52-53 0.012367460483969002 0.0 0.0 0.0 0.0 54-55 0.013681649833309162 0.0 0.0 0.0 0.0 56-57 0.01581720752598692 0.0 0.0 4.69353339050057E-5 0.0 58-59 0.017999700552569685 0.0 0.0 4.69353339050057E-5 0.0 60-61 0.02116783559115757 0.0 0.0 4.69353339050057E-5 0.0 62-63 0.025016532971368036 0.0 0.0 4.69353339050057E-5 0.0 64-65 0.030226355034823668 0.0 0.0 4.69353339050057E-5 0.0 66-67 0.03653915744504694 0.0 0.0 4.69353339050057E-5 0.0 68-69 0.04402534320289535 0.0 0.0 4.69353339050057E-5 0.0 70-71 0.05446845499675912 0.0 0.0 4.69353339050057E-5 0.0 72-73 0.06735220415368318 0.0 0.0 4.69353339050057E-5 0.0 74-75 0.08497642203501282 0.0 0.0 4.69353339050057E-5 0.0 76-77 0.10867876565704071 0.0 0.0 4.69353339050057E-5 0.0 78-79 0.13679303066613913 0.0 0.0 4.69353339050057E-5 0.0 80-81 0.17239348143308592 0.0 0.0 4.69353339050057E-5 0.0 82-83 0.21721672531236638 0.0 0.0 4.69353339050057E-5 0.0 84-85 0.2769888730403911 0.0 0.0 4.69353339050057E-5 0.0 86-87 0.350254929266105 0.0 0.0 4.69353339050057E-5 0.0 88 0.41626947640349554 0.0 0.0 4.69353339050057E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGAGT 1640 0.0 82.038445 1 CGTGTGG 1190 0.0 67.136505 3 GTGGAAT 56705 0.0 64.528244 6 TGGAATC 57375 0.0 64.17606 7 GGAATCA 57930 0.0 63.642345 8 AACGAGT 52570 0.0 63.63381 1 GAATCAC 57975 0.0 63.568626 9 ACGAGTG 55005 0.0 63.49406 2 AGTGGAA 56390 0.0 63.363575 5 CGAGTGG 56210 0.0 62.847458 3 AACGTGT 1005 0.0 62.25594 1 GAGTGGA 57860 0.0 61.843094 4 ACGTGTG 1170 0.0 59.885574 2 ATCGAGT 635 0.0 55.56263 1 TCGAGTG 740 0.0 54.6498 2 ATCTGCG 800 0.0 51.70117 94 CGATTGG 355 0.0 45.009827 3 ACGATTG 320 0.0 42.615723 2 AGTACTT 24700 0.0 41.47883 20-21 GAGTACT 24460 0.0 40.934742 20-21 >>END_MODULE