FastQCFastQC Report
Thu 26 May 2016
SRR2132680_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132680_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2855399
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2128097.452863855454177No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1130893.9605323108959554No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG318481.1153607604401345No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT83360.2919381844708918No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC48470.16974860606170977No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA47640.16684183191210755No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT40360.1413462706963195No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT39120.13700362015956438No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT23550.085.3176041
CGTGTGG17500.069.012223
ATCGAGT8900.067.6739651
AACGTGT14800.066.130731
GTGGAAT798200.064.548816
TGGAATC806800.064.1461647
ACGTGTG17000.063.8996282
GAATCAC812000.063.839559
GGAATCA813400.063.7527778
AACGAGT739300.063.2528341
AGTGGAA797350.063.020465
ACGAGTG777700.062.9952052
CGAGTGG798350.062.2528233
GAGTGGA819550.061.410834
TCGAGTG11400.059.400882
ATCTGCG10750.044.15984394
GTGTGGA30200.044.0362634
AGTACTT318500.041.58527420-21
GAGTACT314800.041.30526420-21
GTACTTT314950.040.56193522-23
Produced by FastQC (version 0.11.5)