FastQCFastQC Report
Thu 26 May 2016
SRR2132679_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132679_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2599058
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT790543.0416404712784404No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA148560.5715917074570863No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG65700.2527838932413205No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT31490.1211592815550865No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT9650.078.5019451
AACGAGT239450.072.4699551
ACGAGTG251650.072.2644962
CGAGTGG263300.070.285113
GAATCAC280700.068.941779
GTGGAAT282750.067.876846
GAGTGGA283900.066.1286854
AGTGGAA283800.065.969845
GGAATCA295700.065.524018
TGGAATC301850.064.048897
CGTGTGG7950.050.835823
ATCGAGT5500.050.4744031
AACGTGT7450.042.947171
GAGTACT118150.040.70998420-21
AGTACTT124150.039.6888520-21
TCGAGTG7300.039.2965772
GTACTTT127300.037.43387222-23
TGAGTAC275900.035.5304818-19
GCTGAGT281950.035.2014316-17
ATCACTG283250.035.17259610-11
Produced by FastQC (version 0.11.5)