##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132679_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2599058
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.06175352762424	31.0	28.0	34.0	16.0	34.0
2	30.2168166312564	33.0	30.0	34.0	19.0	34.0
3	30.696990986734424	33.0	31.0	34.0	25.0	34.0
4	35.19700022084925	37.0	35.0	37.0	32.0	37.0
5	34.68810353597342	37.0	35.0	37.0	32.0	37.0
6	35.25978758457872	37.0	35.0	37.0	32.0	37.0
7	35.73700817757818	37.0	35.0	37.0	35.0	37.0
8	35.90726717141364	37.0	35.0	37.0	35.0	37.0
9	37.83378247041813	39.0	39.0	39.0	35.0	39.0
10-11	37.93367924070952	39.0	39.0	39.0	35.0	39.0
12-13	37.94815833274979	39.0	39.0	39.0	35.0	39.0
14-15	39.38191914147356	41.0	39.0	41.0	37.0	41.0
16-17	39.36010777751016	41.0	39.0	41.0	36.5	41.0
18-19	39.37171929214354	41.0	39.5	41.0	36.5	41.0
20-21	39.2912387872837	41.0	39.0	41.0	36.0	41.0
22-23	39.24719879279339	40.5	39.0	41.0	36.0	41.0
24-25	39.17588891821575	41.0	39.0	41.0	36.0	41.0
26-27	39.14036566325184	41.0	39.0	41.0	36.0	41.0
28-29	38.998339013596464	41.0	39.0	41.0	35.5	41.0
30-31	38.92357711909469	41.0	39.0	41.0	35.0	41.0
32-33	38.823380432448985	41.0	39.0	41.0	35.0	41.0
34-35	38.7602589091894	40.5	39.0	41.0	35.0	41.0
36-37	38.67181936686292	40.0	38.5	41.0	35.0	41.0
38-39	38.446583723795314	40.0	38.0	41.0	35.0	41.0
40-41	38.31011043231817	40.0	38.0	41.0	34.0	41.0
42-43	38.2074386181455	40.0	38.0	41.0	34.0	41.0
44-45	38.11378776464396	40.0	38.0	41.0	34.0	41.0
46-47	38.00887821664619	40.0	38.0	41.0	34.0	41.0
48-49	37.89740763769027	40.0	37.0	41.0	33.0	41.0
50-51	37.726546887372265	40.0	37.0	41.0	33.0	41.0
52-53	37.47021555502032	40.0	37.0	41.0	33.0	41.0
54-55	37.0432714468088	40.0	36.5	41.0	32.0	41.0
56-57	36.68888631958194	40.0	36.0	41.0	31.0	41.0
58-59	36.44577477686146	39.5	36.0	41.0	31.0	41.0
60-61	36.22941715806265	39.0	35.0	41.0	30.5	41.0
62-63	35.95081044747751	39.0	35.0	41.0	30.0	41.0
64-65	35.710626696287655	39.0	35.0	40.5	30.0	41.0
66-67	35.43874876974658	38.0	35.0	40.0	29.0	41.0
68-69	35.1455594680842	37.5	35.0	40.0	29.0	41.0
70-71	34.80786115584954	37.0	35.0	40.0	29.0	41.0
72-73	34.4637320521512	36.5	34.0	39.0	28.5	41.0
74-75	34.107939684301	36.0	34.0	39.0	28.0	40.5
76-77	33.730178203025865	35.5	34.0	38.5	27.5	40.0
78-79	33.62219965849165	35.0	34.0	37.5	28.5	39.5
80-81	33.484106164618105	35.0	34.0	37.0	29.0	39.0
82-83	33.16382262342741	35.0	34.0	37.0	29.0	39.0
84-85	32.878154123532454	35.0	34.0	36.0	28.5	38.0
86-87	32.66159008379189	35.0	34.0	36.0	28.5	37.0
88-89	32.4397928018536	35.0	34.0	36.0	27.5	37.0
90-91	32.23937288048208	35.0	34.0	35.0	27.0	36.5
92-93	32.070499196247255	35.0	34.0	35.0	27.0	36.0
94-95	31.913231447701435	35.0	34.0	35.0	26.5	36.0
96-97	31.760020361223184	35.0	34.0	35.0	26.0	36.0
98-99	31.621675430098136	35.0	34.0	35.0	25.5	36.0
100	31.512569938800905	35.0	34.0	35.0	25.0	36.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	3.0283707245935396
1101	2	2.367597828075006
1101	3	2.133351641657967
1101	4	1.0623322590884428
1101	5	1.6382498407418993
1101	6	1.1035306020278952
1101	7	0.5453726929221006
1101	8	0.49897241491206046
1101	9	0.4406437567008865
1101	10-11	0.34498440646495965
1101	12-13	0.27919435138493043
1101	14-15	0.3993415822384705
1101	16-17	0.41260179746711856
1101	18-19	0.3976584908174772
1101	20-21	0.40166963343335027
1101	22-23	0.38994135392050566
1101	24-25	0.47043561469550355
1101	26-27	0.4162947364120555
1101	28-29	0.3897378591671483
1101	30-31	0.3783571043688596
1101	32-33	0.40694727362756566
1101	34-35	0.4097181111770709
1101	36-37	0.40765400298298005
1101	38-39	0.3451263982637016
1101	40-41	0.3512660972134327
1101	42-43	0.3028517353829159
1101	44-45	0.2947761186786053
1101	46-47	0.2821795481147831
1101	48-49	0.19185926891674399
1101	50-51	0.25856374900244816
1101	52-53	0.23992229715133107
1101	54-55	0.12759381745799203
1101	56-57	0.09013747591325938
1101	58-59	-0.11575418534392412
1101	60-61	-0.04722857502741107
1101	62-63	-0.007691118607823455
1101	64-65	0.007597036289382686
1101	66-67	0.025745630058537472
1101	68-69	0.030489362323400826
1101	70-71	-0.0061238413427133764
1101	72-73	-0.059386660028003746
1101	74-75	-0.032521555077337894
1101	76-77	-0.05690060072839742
1101	78-79	0.03457680106610894
1101	80-81	0.016930277838241636
1101	82-83	0.05307259619988258
1101	84-85	0.06035268635161373
1101	86-87	0.04929788003010316
1101	88-89	-0.07482036228492461
1101	90-91	-0.17681167353371308
1101	92-93	-0.11360180357675276
1101	94-95	-0.037597043587542345
1101	96-97	-0.04616067186139716
1101	98-99	-0.0014347203897884242
1101	100	-3.623165978190457E-4
1102	1	2.85019837887085
1102	2	2.322036653607384
1102	3	2.086276197122153
1102	4	1.0475027490557238
1102	5	1.5798311147907071
1102	6	1.044877251922962
1102	7	0.5458778304584939
1102	8	0.4733935431565115
1102	9	0.3708918305783939
1102	10-11	0.3324854448330967
1102	12-13	0.28622299475325974
1102	14-15	0.3878991349855525
1102	16-17	0.3749600786983436
1102	18-19	0.33189510810038314
1102	20-21	0.4039402320361134
1102	22-23	0.32353028487581525
1102	24-25	0.3971633181423968
1102	26-27	0.30664988251078285
1102	28-29	0.36522890984515755
1102	30-31	0.43157894899994886
1102	32-33	0.3653604952194911
1102	34-35	0.4112846356937112
1102	36-37	0.4167763527247317
1102	38-39	0.34683970569405886
1102	40-41	0.33675317184877684
1102	42-43	0.3500823302650389
1102	44-45	0.3836220218895292
1102	46-47	0.34374721786075924
1102	48-49	0.34750920806635577
1102	50-51	0.3063053957508117
1102	52-53	0.3282266023264526
1102	54-55	0.32778171840586623
1102	56-57	0.26281937448840864
1102	58-59	0.2242329483003047
1102	60-61	0.30792918434485017
1102	62-63	0.363346213179895
1102	64-65	0.2975368418530948
1102	66-67	0.24086889410191503
1102	68-69	0.2714101603062886
1102	70-71	0.26573636191129424
1102	72-73	0.2635434791012372
1102	74-75	0.29473881442311267
1102	76-77	0.23442164583516956
1102	78-79	0.3176125674382533
1102	80-81	0.37174540932857525
1102	82-83	0.4119156551558021
1102	84-85	0.33077725324120877
1102	86-87	0.339052627556768
1102	88-89	0.33353071179769955
1102	90-91	0.3366994786073221
1102	92-93	0.37114144326667287
1102	94-95	0.27115466585540915
1102	96-97	0.2728167458830413
1102	98-99	0.2794564293263626
1102	100	0.21510424741041945
1103	1	2.7372821595043924
1103	2	2.2471665559014937
1103	3	2.0432336411490155
1103	4	1.0311612206476752
1103	5	1.6319374351459572
1103	6	1.0615568038169272
1103	7	0.5271005600520695
1103	8	0.40893910648645715
1103	9	0.3969508765735199
1103	10-11	0.30008632116884826
1103	12-13	0.2531758721748929
1103	14-15	0.4158787659796488
1103	16-17	0.3837456313378027
1103	18-19	0.34888170895462167
1103	20-21	0.3952368177884651
1103	22-23	0.35675996644191343
1103	24-25	0.4143832472705782
1103	26-27	0.29861679382578643
1103	28-29	0.414733271400344
1103	30-31	0.4605162584013911
1103	32-33	0.4052061315993427
1103	34-35	0.3622416038571572
1103	36-37	0.3541115005120119
1103	38-39	0.35709164893452083
1103	40-41	0.30709164384057175
1103	42-43	0.3156381645109434
1103	44-45	0.28567396255206745
1103	46-47	0.3386100171529307
1103	48-49	0.2623025954851954
1103	50-51	0.2518499765767075
1103	52-53	0.32777520286975914
1103	54-55	0.35918679654453456
1103	56-57	0.2882788562576408
1103	58-59	0.2936552122369349
1103	60-61	0.373116980723708
1103	62-63	0.36746789663681767
1103	64-65	0.2155457598859556
1103	66-67	0.17615317974517097
1103	68-69	0.13696029944832588
1103	70-71	0.18182462918186104
1103	72-73	0.14415141368303352
1103	74-75	0.15238653660515666
1103	76-77	0.11386461601203735
1103	78-79	0.12469493547559551
1103	80-81	0.20705540185522864
1103	82-83	0.24827720323497715
1103	84-85	0.27887114932621415
1103	86-87	0.24863144371697388
1103	88-89	0.20547327621484612
1103	90-91	0.19941262082892308
1103	92-93	0.18268223586203902
1103	94-95	0.24300885094129043
1103	96-97	0.2569060987745644
1103	98-99	0.2645805023594292
1103	100	0.29569141393446685
1104	1	1.5086935005816997
1104	2	1.7661603726005168
1104	3	1.8390105476243122
1104	4	0.9278165517406691
1104	5	1.5732933882027211
1104	6	1.0135869594815858
1104	7	0.5190347612383732
1104	8	0.4231438372105387
1104	9	0.3292639551523493
1104	10-11	0.2809328459586311
1104	12-13	0.2221771952782703
1104	14-15	0.33203229314502636
1104	16-17	0.246284179353772
1104	18-19	0.30170993405495494
1104	20-21	0.4010424079719357
1104	22-23	0.30283105703161795
1104	24-25	0.3407453249795509
1104	26-27	0.2952029895003818
1104	28-29	0.3993985319841329
1104	30-31	0.3647984150567041
1104	32-33	0.3485113273380023
1104	34-35	0.322763781855528
1104	36-37	0.3293743760673067
1104	38-39	0.2752445889747719
1104	40-41	0.3163102733655734
1104	42-43	0.2477656388752152
1104	44-45	0.36867777376551913
1104	46-47	0.41319116457137994
1104	48-49	0.3685557012400764
1104	50-51	0.3078720093874949
1104	52-53	0.3578500979793162
1104	54-55	0.32403004975324023
1104	56-57	0.2406165354431593
1104	58-59	0.2863273041268002
1104	60-61	0.3058986756793729
1104	62-63	0.25782890846825524
1104	64-65	0.2542538806042316
1104	66-67	0.25486061835282925
1104	68-69	0.23426844866225593
1104	70-71	0.20919495564830015
1104	72-73	0.1866041623816912
1104	74-75	0.2614643772214009
1104	76-77	0.2738901919731802
1104	78-79	0.15754299278309247
1104	80-81	0.1982361576656828
1104	82-83	0.28378351578635375
1104	84-85	0.3039935387788688
1104	86-87	0.15256205840383785
1104	88-89	0.14525492431525322
1104	90-91	0.15916175073944316
1104	92-93	0.14864007058790918
1104	94-95	0.13259458348188602
1104	96-97	0.22401580798187837
1104	98-99	0.2614773452390722
1104	100	0.27054715869249435
1105	1	2.387935756536283
1105	2	2.1468478471236097
1105	3	2.0814314242917646
1105	4	1.0500883253823048
1105	5	1.6214603596573625
1105	6	1.1033084992031306
1105	7	0.6046243981978705
1105	8	0.46679290346234836
1105	9	0.3793756484147295
1105	10-11	0.3461712786628226
1105	12-13	0.27707265736551534
1105	14-15	0.39690660082755613
1105	16-17	0.30451990574018595
1105	18-19	0.2892963685591923
1105	20-21	0.3780820254225219
1105	22-23	0.3704213434575223
1105	24-25	0.44196544298048934
1105	26-27	0.29320195359522927
1105	28-29	0.3456511357709786
1105	30-31	0.38797766529075517
1105	32-33	0.4171439076144168
1105	34-35	0.40625365404402203
1105	36-37	0.4356121206612116
1105	38-39	0.3994364957009253
1105	40-41	0.24589882829659615
1105	42-43	0.30079756245508804
1105	44-45	0.38042650880192497
1105	46-47	0.39877076658933674
1105	48-49	0.29469251676044905
1105	50-51	0.33977617382591063
1105	52-53	0.3115780164332591
1105	54-55	0.29381879865157856
1105	56-57	0.23623279304572264
1105	58-59	0.2509631728722539
1105	60-61	0.32166169897664787
1105	62-63	0.31143188878355943
1105	64-65	0.2574088412934401
1105	66-67	0.24081372588127437
1105	68-69	0.1413677335012764
1105	70-71	0.1181356522602286
1105	72-73	0.1935928758772505
1105	74-75	0.17129509835349666
1105	76-77	0.11076137199503933
1105	78-79	0.0915976264952576
1105	80-81	0.05636901031908792
1105	82-83	0.16923322452373668
1105	84-85	0.19192334280456436
1105	86-87	0.17466058906400406
1105	88-89	0.1735069933207427
1105	90-91	0.20117237905144236
1105	92-93	0.24158267072228767
1105	94-95	0.29028324973219455
1105	96-97	0.37739730280173234
1105	98-99	0.3404089543076658
1105	100	0.3291486618145463
1106	1	1.734974330781629
1106	2	1.1846767885196634
1106	3	1.6603730019032383
1106	4	0.8558918919476639
1106	5	1.5646887149776134
1106	6	1.0049027778832595
1106	7	0.5348313282234187
1106	8	0.421316048130592
1106	9	0.3600975432236808
1106	10-11	0.3432107909683566
1106	12-13	0.2669812176791524
1106	14-15	0.3255595887610738
1106	16-17	0.3300912751749152
1106	18-19	0.3000304702546046
1106	20-21	0.36048952360994235
1106	22-23	0.2768602793704602
1106	24-25	0.4097891614994893
1106	26-27	0.3621416611383239
1106	28-29	0.42399538086250743
1106	30-31	0.3396419958849535
1106	32-33	0.35188184990978755
1106	34-35	0.310486514473979
1106	36-37	0.3241351055566284
1106	38-39	0.33026905135209716
1106	40-41	0.39112343621769696
1106	42-43	0.3966631378248877
1106	44-45	0.3993497851617249
1106	46-47	0.3841944006224054
1106	48-49	0.355679410546621
1106	50-51	0.25859835150540533
1106	52-53	0.3304828039293426
1106	54-55	0.3910306114317095
1106	56-57	0.3448307803921935
1106	58-59	0.34265401728378464
1106	60-61	0.3506999875675376
1106	62-63	0.4128444863872147
1106	64-65	0.35376553656186616
1106	66-67	0.3339577465645931
1106	68-69	0.3709291873372109
1106	70-71	0.35624207791200035
1106	72-73	0.3468252799863123
1106	74-75	0.3738295856824223
1106	76-77	0.4255548232455695
1106	78-79	0.479157223577225
1106	80-81	0.4357863962660957
1106	82-83	0.48008155774208205
1106	84-85	0.527896044647882
1106	86-87	0.4441398930354552
1106	88-89	0.390364933485813
1106	90-91	0.4124305537920847
1106	92-93	0.45606913421116957
1106	94-95	0.4393422543232468
1106	96-97	0.4358786680847899
1106	98-99	0.5061437338272086
1106	100	0.5588613854208617
1107	1	1.750801969629606
1107	2	1.1450730027119036
1107	3	1.4152159618774007
1107	4	0.7451792446544374
1107	5	1.4945431005383298
1107	6	1.0176814545155963
1107	7	0.5127345762697999
1107	8	0.3624742051321732
1107	9	0.3752347037406736
1107	10-11	0.21541217267660073
1107	12-13	0.16893083756340843
1107	14-15	0.28362911703249694
1107	16-17	0.22822519721382406
1107	18-19	0.2645302984010627
1107	20-21	0.2613912849569715
1107	22-23	0.204422060646138
1107	24-25	0.2683797427691701
1107	26-27	0.2432804387263161
1107	28-29	0.25935338691047605
1107	30-31	0.2860916231405426
1107	32-33	0.25915248324717766
1107	34-35	0.19907908167103017
1107	36-37	0.2823435042082778
1107	38-39	0.3125949625588902
1107	40-41	0.3289073089263965
1107	42-43	0.25258696840418793
1107	44-45	0.291450968595214
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2215	4	-1.9060053008345719
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2216	3	-5.7558297613429765
2216	4	-3.132320473763116
2216	5	-4.290426868599226
2216	6	-2.07506609784933
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2216	38-39	-1.132905788871163
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2216	56-57	-1.0484365825208215
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2216	80-81	-0.8618501007121964
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2216	84-85	-0.8647671357117233
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2216	88-89	-1.057853827100935
2216	90-91	-0.9873145076253707
2216	92-93	-0.9437009864921109
2216	94-95	-0.9514798932638975
2216	96-97	-0.9308727983592426
2216	98-99	-0.9080720818176502
2216	100	-0.9589657905181639
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2688.0
3	945.0
4	1064.0
5	1391.0
6	1232.0
7	1248.0
8	1418.0
9	1796.0
10	2149.0
11	3790.0
12	3044.0
13	3765.0
14	3688.0
15	3795.0
16	4211.0
17	5007.0
18	6656.0
19	10462.0
20	20449.0
21	27137.0
22	13820.0
23	10051.0
24	11236.0
25	12897.0
26	13968.0
27	15948.0
28	19094.0
29	23250.0
30	28828.0
31	37031.0
32	47894.0
33	66649.0
34	97964.0
35	165857.0
36	282478.0
37	530386.0
38	763753.0
39	347127.0
40	4892.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	16.427104880900544	32.533318241614566	29.551895300431074	21.48768157705381
2	19.874256211631494	33.10592162381201	25.492493296038838	21.52732886851766
3	19.35447381320463	27.6427459487245	24.52546268686578	28.47731755120509
4	25.716548072417005	28.43372483415145	25.979412541005242	19.8703145524263
5	19.894515628354583	33.14200760429355	26.314110727809847	20.64936603954202
6	29.137056579730043	26.190835294941472	26.336503456252224	18.335604669076258
7	20.19200802752382	25.591079537278503	34.13178928673389	20.08512314846379
8	27.164995933141935	25.569071563620362	27.618929627580453	19.647002875657257
9	27.416356233681586	26.623338147898203	27.012363710236553	18.947941908183658
10-11	19.43379101197434	34.55082572224244	26.4724757970003	19.54290746878292
12-13	19.286756971179557	26.76739033911517	30.372754282513124	23.573098407192145
14-15	19.340911207060405	30.828342422523853	26.373035923015188	23.457710447400558
16-17	23.50418767429405	26.775106038500134	30.312371107048097	19.408335180157717
18-19	19.56435754800393	26.755539891760783	30.276969578978232	23.403132981257055
20-21	23.687884948642733	30.722672301550414	26.201209058085883	19.38823369172097
22-23	23.331697483298267	26.666311409544196	30.47929123510782	19.522699872049724
24-25	19.28377512160175	30.69094648907412	26.46247217261023	23.562806216713902
26-27	19.584793911962628	28.693764155693046	32.32498979895525	19.396452133389072
28-29	23.69565100291031	26.722792604642642	30.153083681795867	19.428472710651178
30-31	22.58704053708794	27.189095865026182	30.390830134549024	19.833033463336854
32-33	20.561010702673524	27.928431582092273	31.022593677971745	20.487964037262458
34-35	20.492887242438606	27.862051903199394	31.140938486921083	20.504122367440917
36-37	20.558777168470634	27.922351144456588	31.18138440662392	20.33748728044886
38-39	20.92568610297206	27.83992402511435	30.92923203374233	20.30515783817126
40-41	20.532721348715803	28.14541188529636	30.92031246152331	20.40155430446453
42-43	20.489481321182843	27.837896179583836	31.178119732051023	20.49450276718229
44-45	20.426450430692427	28.09393952510809	30.965761034238966	20.513849009960513
46-47	20.68634620042482	27.939824420559567	31.12422900471702	20.2496003742986
48-49	20.503106612922338	28.059632808664347	31.017186795394487	20.420073783018825
50-51	20.756702656974763	28.161827673256308	30.661749271731605	20.419720398037324
52-53	20.712357197851606	28.127660917065768	30.690837076172308	20.469144808910322
54-55	20.77621240022193	28.39439742241108	30.087132909429638	20.742257267937354
56-57	21.00113695607425	28.73378490945713	29.50779998037741	20.757278154091214
58-59	21.417060290249978	29.000219504504653	28.937157649443417	20.64556255580195
60-61	21.26782894067277	29.09914369524485	28.809251762140093	20.823775601942284
62-63	20.914731157765537	29.13119008547469	28.81941812571301	21.13466063104676
64-65	20.92919363927698	29.112161103224093	29.165992491694904	20.792652765804025
66-67	20.982595167466343	28.867525717062865	29.371265768572023	20.778613346898773
68-69	21.135610696136204	28.842619234659413	29.310805592608602	20.71096447659578
70-71	20.653215361208186	29.127222270535007	29.482366132754812	20.737196235501994
72-73	20.67812131461035	28.717825887042626	29.649819445967495	20.95423335237952
74-75	20.6806782812151	29.006500743263636	29.36469475594654	20.94812621957472
76-77	20.952225394665177	28.888661873565162	29.352754301892286	20.806358429877374
78-79	20.995954235353945	29.07457294546174	28.88537808283437	21.044094736349948
80-81	21.23305026293629	29.271245238470406	28.677322642341814	20.81838185625149
82-83	21.100155105335798	29.32408549994055	28.775252714728154	20.80050667999549
84-85	20.992455923628466	29.466146887955468	28.539976024716356	21.001421163699703
86-87	21.04258573421192	29.56080996585061	28.591486700976382	20.80511759896109
88-89	21.511273895018615	29.465823940585157	28.377517582605105	20.64538458179112
90-91	21.287961034261247	29.60652368686195	28.258183226470752	20.84733205240605
92-93	21.086849071592674	29.684407501447314	28.143183422519463	21.08556000444055
94-95	21.084786576180246	29.716012086917136	28.254746439088475	20.944454897814143
96-97	21.260346240721574	29.576414996325184	28.264813738807206	20.89842502414604
98-99	21.513645685439347	29.743015556042923	27.844316446004026	20.899022312513708
100	21.28040329698828	30.024763580145734	27.736748829732306	20.958084293133687
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	13893.0
1	7670.5
2	1143.5
3	727.0
4	588.0
5	568.5
6	585.0
7	591.5
8	571.0
9	587.0
10	617.0
11	910.5
12	7822.0
13	9104.0
14	3611.0
15	3702.5
16	4345.0
17	5138.5
18	5971.0
19	7078.5
20	8644.5
21	10695.5
22	13378.0
23	16689.0
24	20349.5
25	24547.0
26	29659.0
27	34963.0
28	40172.5
29	45265.5
30	50397.0
31	56068.0
32	61253.5
33	66297.0
34	71307.0
35	76406.5
36	81520.5
37	85443.5
38	88290.0
39	89662.5
40	89459.5
41	88152.5
42	85920.0
43	83448.0
44	81392.0
45	79363.0
46	77679.5
47	76978.0
48	75973.0
49	75769.5
50	80840.0
51	78070.5
52	67020.5
53	61987.0
54	59790.5
55	57164.5
56	53167.5
57	48503.5
58	43603.0
59	37958.5
60	31940.0
61	26415.0
62	21676.0
63	17271.0
64	13619.0
65	10727.0
66	8437.5
67	6735.5
68	5212.0
69	3903.0
70	2906.0
71	2177.5
72	1608.5
73	1167.0
74	864.5
75	708.0
76	578.5
77	443.5
78	339.0
79	244.5
80	184.0
81	132.5
82	91.0
83	60.0
84	36.5
85	21.5
86	11.0
87	6.0
88	5.0
89	4.0
90	2.5
91	1.5
92	0.5
93	0.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06183009382630169
2	0.06590849453917536
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	7.695095684667291E-5
18-19	0.0
20-21	3.847547842333645E-4
22-23	5.7713217635004685E-5
24-25	0.0
26-27	0.0022892909661885188
28-29	8.46460525313402E-4
30-31	0.005925223677193814
32-33	0.0013274040056051077
34-35	0.002539381575940206
36-37	0.007868235337572305
38-39	0.011715783179905952
40-41	0.0031549892307135893
42-43	0.007887473076783974
44-45	0.0017506342682618086
46-47	0.0024431928798818647
48-49	0.003308891144406935
50-51	0.0098689602155858
52-53	0.0036551704502169632
54-55	0.001539019136933458
56-57	5.00181219503374E-4
58-59	8.656982645250703E-4
60-61	0.0060983633300988275
62-63	0.0016544455722034676
64-65	0.007791284380725632
66-67	0.011427217091730927
68-69	0.011792734136752622
70-71	0.009695820562680786
72-73	0.00434772906183702
74-75	0.0018275852251084816
76-77	0.004405442279472024
78-79	0.015948085806472962
80-81	0.009022499690272398
82-83	0.006887110637777226
84-85	0.005059525412668744
86-87	0.007041012551470571
88-89	0.004290015844202015
90-91	0.0061368388085221645
92-93	0.010753896219322538
94-95	0.008272227861017339
96-97	0.011080937785920899
98-99	0.002616332532786879
100	0.003078038273866916
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2599058.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.67360232529182
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.26743488365291	81.61904330772713
2	3.2769515200044945	5.614964827282512
3	0.6619664472960486	1.7013915047498374
4	0.27469781473454774	0.9413740535677734
5	0.13415132729490054	0.5746613733036687
6	0.07867163271528106	0.40440493053182447
7	0.04487260400306364	0.26910783414611406
8	0.033458852158616555	0.22932323152784376
9	0.024212856103206393	0.18669623444510575
>10	0.19251589267131683	2.7883578785092076
>50	0.004973590096576197	0.28621825766954834
>100	0.0055074018092681285	1.049848361917994
>500	2.700819045381726E-4	0.18152458957610731
>1k	1.800546030251869E-4	0.2780908891930782
>5k	4.501365075629673E-5	0.2533708420466681
>10k+	9.002730151259345E-5	3.621621883805571
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	79054	3.0416404712784404	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	14856	0.5715917074570863	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	6570	0.2527838932413205	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3149	0.1211592815550865	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.004116876191297001	3.8475478423336456E-5	0.0	0.0	0.0
2	0.004116876191297001	3.8475478423336456E-5	0.0	0.0	0.0
3	0.004116876191297001	3.8475478423336456E-5	0.0	0.0	0.0
4	0.004116876191297001	3.8475478423336456E-5	0.0	3.8475478423336456E-5	0.0
5	0.004116876191297001	3.8475478423336456E-5	0.0	7.695095684667291E-5	0.0
6	0.004116876191297001	3.8475478423336456E-5	0.0	7.695095684667291E-5	0.0
7	0.004116876191297001	3.8475478423336456E-5	0.0	7.695095684667291E-5	0.0
8	0.004116876191297001	3.8475478423336456E-5	0.0	1.1542643527000937E-4	0.0
9	0.004116876191297001	3.8475478423336456E-5	3.8475478423336456E-5	1.1542643527000937E-4	0.0
10-11	0.004116876191297001	3.8475478423336456E-5	3.8475478423336456E-5	1.1542643527000937E-4	0.0
12-13	0.004116876191297001	3.8475478423336456E-5	3.8475478423336456E-5	1.3466417448167758E-4	0.0
14-15	0.004116876191297001	3.8475478423336456E-5	3.8475478423336456E-5	1.9237739211668228E-4	0.0
16-17	0.004155351669720337	7.695095684667291E-5	3.8475478423336456E-5	1.9237739211668228E-4	0.0
18-19	0.004155351669720337	1.1542643527000937E-4	3.8475478423336456E-5	1.9237739211668228E-4	0.0
20-21	0.004155351669720337	1.5390191369334583E-4	3.8475478423336456E-5	1.9237739211668228E-4	0.0
22-23	0.004193827148143673	1.5390191369334583E-4	3.8475478423336456E-5	1.9237739211668228E-4	0.0
24-25	0.004193827148143673	1.9237739211668228E-4	3.8475478423336456E-5	1.9237739211668228E-4	0.0
26-27	0.004193827148143673	2.116151313283505E-4	3.8475478423336456E-5	1.9237739211668228E-4	0.0
28-29	0.004251540365778678	2.6932834896335517E-4	3.8475478423336456E-5	1.9237739211668228E-4	0.0
30-31	0.004328491322625351	2.885660881750234E-4	3.8475478423336456E-5	1.9237739211668228E-4	0.0
32-33	0.004463155497107029	3.0780382738669165E-4	3.8475478423336456E-5	1.9237739211668228E-4	0.0
34-35	0.00477095932449372	3.0780382738669165E-4	3.8475478423336456E-5	1.9237739211668228E-4	3.8475478423336456E-5
36-37	0.004924861238187066	3.2704156659835984E-4	3.8475478423336456E-5	1.9237739211668228E-4	3.8475478423336456E-5
38-39	0.005559706632172118	3.462793058100281E-4	3.8475478423336456E-5	2.50090609751687E-4	7.695095684667291E-5
40-41	0.006598544549602202	3.462793058100281E-4	3.8475478423336456E-5	2.885660881750234E-4	7.695095684667291E-5
42-43	0.007445005074915604	3.8475478423336456E-4	3.8475478423336456E-5	3.0780382738669165E-4	7.695095684667291E-5
44-45	0.008945548733425726	4.23230262656701E-4	3.8475478423336456E-5	3.462793058100281E-4	7.695095684667291E-5
46-47	0.01052304334878252	4.809434802917057E-4	3.8475478423336456E-5	3.462793058100281E-4	7.695095684667291E-5
48-49	0.013639557101072772	5.00181219503374E-4	3.8475478423336456E-5	3.655170450216963E-4	7.695095684667291E-5
50-51	0.01835280320793149	5.386566979267103E-4	3.8475478423336456E-5	3.8475478423336456E-4	7.695095684667291E-5
52-53	0.023239188967695217	5.578944371383786E-4	3.8475478423336456E-5	4.23230262656701E-4	7.695095684667291E-5
54-55	0.02745225385505056	5.96369915561715E-4	3.8475478423336456E-5	5.00181219503374E-4	7.695095684667291E-5
56-57	0.03306967370485768	6.73320872408388E-4	3.8475478423336456E-5	5.00181219503374E-4	7.695095684667291E-5
58-59	0.04151504121878004	7.69509568466729E-4	3.8475478423336456E-5	5.00181219503374E-4	7.695095684667291E-5
60-61	0.05469289257877277	8.46460525313402E-4	3.8475478423336456E-5	5.386566979267103E-4	7.695095684667291E-5
62-63	0.0681785477661522	8.46460525313402E-4	3.8475478423336456E-5	5.386566979267103E-4	7.695095684667291E-5
64-65	0.08308779565519508	8.656982645250703E-4	3.8475478423336456E-5	5.386566979267103E-4	7.695095684667291E-5
66-67	0.10124822147100987	8.849360037367385E-4	3.8475478423336456E-5	5.386566979267103E-4	7.695095684667291E-5
68-69	0.12898904141423545	9.23411482160075E-4	3.8475478423336456E-5	5.386566979267103E-4	7.695095684667291E-5
70-71	0.16981152402139543	9.618869605834114E-4	3.8475478423336456E-5	5.386566979267103E-4	9.618869605834114E-5
72-73	0.21303872403001395	0.001019600178218416	3.8475478423336456E-5	5.386566979267103E-4	1.1542643527000937E-4
74-75	0.25868987917930264	0.0010773133958534207	3.8475478423336456E-5	5.386566979267103E-4	1.1542643527000937E-4
76-77	0.31422923228338884	0.0010773133958534207	3.8475478423336456E-5	5.386566979267103E-4	1.1542643527000937E-4
78-79	0.3897565964283983	0.0010773133958534207	3.8475478423336456E-5	6.156076547733833E-4	1.1542643527000937E-4
80-81	0.5081841190154279	0.0011157888742767573	3.8475478423336456E-5	6.348453939850516E-4	1.1542643527000937E-4
82-83	0.641655553665982	0.0011542643527000936	3.8475478423336456E-5	6.540831331967198E-4	1.1542643527000937E-4
84-85	0.7693171910746124	0.0011542643527000936	3.8475478423336456E-5	6.540831331967198E-4	1.1542643527000937E-4
86-87	0.9190445153590262	0.0012504530487584347	3.8475478423336456E-5	6.540831331967198E-4	1.1542643527000937E-4
88	1.051611776266632	0.0013081662663934396	3.8475478423336456E-5	6.540831331967198E-4	1.1542643527000937E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	965	0.0	78.501945	1
AACGAGT	23945	0.0	72.469955	1
ACGAGTG	25165	0.0	72.264496	2
CGAGTGG	26330	0.0	70.28511	3
GAATCAC	28070	0.0	68.94177	9
GTGGAAT	28275	0.0	67.87684	6
GAGTGGA	28390	0.0	66.128685	4
AGTGGAA	28380	0.0	65.96984	5
GGAATCA	29570	0.0	65.52401	8
TGGAATC	30185	0.0	64.04889	7
CGTGTGG	795	0.0	50.83582	3
ATCGAGT	550	0.0	50.474403	1
AACGTGT	745	0.0	42.94717	1
GAGTACT	11815	0.0	40.709984	20-21
AGTACTT	12415	0.0	39.68885	20-21
TCGAGTG	730	0.0	39.296577	2
GTACTTT	12730	0.0	37.433872	22-23
TGAGTAC	27590	0.0	35.53048	18-19
GCTGAGT	28195	0.0	35.20143	16-17
ATCACTG	28325	0.0	35.172596	10-11
>>END_MODULE