##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2132678_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2806306 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.248786126673284 31.0 28.0 34.0 16.0 34.0 2 30.359610463007243 33.0 30.0 34.0 23.0 34.0 3 30.81065927949411 33.0 31.0 34.0 25.0 34.0 4 35.23260328702572 37.0 35.0 37.0 32.0 37.0 5 34.69493098756871 37.0 35.0 37.0 32.0 37.0 6 35.22228188943045 37.0 35.0 37.0 32.0 37.0 7 35.61059057707891 37.0 35.0 37.0 33.0 37.0 8 35.80507115047325 37.0 35.0 37.0 35.0 37.0 9 37.70838212226322 39.0 39.0 39.0 35.0 39.0 10-11 37.790247036495664 39.0 39.0 39.0 35.0 39.0 12-13 37.77944725201029 39.0 39.0 39.0 35.0 39.0 14-15 39.20912437916606 41.0 39.5 41.0 36.0 41.0 16-17 39.12796127720925 41.0 39.0 41.0 36.0 41.0 18-19 38.96937397418528 41.0 39.0 41.0 35.5 41.0 20-21 38.804455750727115 41.0 39.0 41.0 35.0 41.0 22-23 38.69559698764141 40.5 39.0 41.0 35.0 41.0 24-25 38.563826254157604 40.5 38.5 41.0 35.0 41.0 26-27 38.53075947526749 40.0 38.5 41.0 35.0 41.0 28-29 38.378123412058414 40.5 38.0 41.0 35.0 41.0 30-31 38.236880974491015 40.5 38.0 41.0 34.5 41.0 32-33 38.08952730030153 40.0 38.0 41.0 34.0 41.0 34-35 37.99274651445708 40.0 38.0 41.0 34.0 41.0 36-37 37.80950865657559 40.0 38.0 41.0 33.5 41.0 38-39 37.419055334664144 40.0 36.0 41.0 33.0 41.0 40-41 37.2550641305688 40.0 35.0 41.0 33.0 41.0 42-43 37.1344593212572 40.0 35.0 41.0 33.0 41.0 44-45 36.99302196553049 40.0 35.0 41.0 33.0 41.0 46-47 36.855394066078325 40.0 35.0 41.0 33.0 41.0 48-49 36.69248025696414 40.0 35.0 41.0 32.5 41.0 50-51 36.441137032098425 39.0 35.0 41.0 31.5 41.0 52-53 35.98184535114845 39.0 35.0 41.0 30.5 41.0 54-55 35.205884176565206 39.0 35.0 41.0 27.0 41.0 56-57 34.67451589384764 39.0 35.0 41.0 23.0 41.0 58-59 34.4287725572336 38.5 35.0 41.0 22.0 41.0 60-61 34.17234364320926 38.0 35.0 40.0 20.5 41.0 62-63 33.89307206698058 37.5 34.5 40.0 20.0 41.0 64-65 33.58875796153377 37.0 34.0 40.0 19.0 41.0 66-67 33.20500437229582 36.0 34.0 39.5 17.0 41.0 68-69 32.93203342757347 36.0 34.0 39.0 16.0 41.0 70-71 32.626404426317016 35.0 34.0 39.0 12.5 41.0 72-73 32.28371870351986 35.0 33.0 38.5 9.0 40.0 74-75 31.93169205353942 35.0 33.0 37.0 7.0 40.0 76-77 31.55570080383251 35.0 33.0 37.0 2.0 39.0 78-79 31.425972969448093 35.0 33.0 36.5 2.0 39.0 80-81 31.294851131701247 35.0 33.0 36.0 2.0 39.0 82-83 30.9605736152793 35.0 33.0 36.0 2.0 37.5 84-85 30.668117090581 35.0 33.0 35.5 2.0 37.0 86-87 30.527031799098175 35.0 33.0 35.0 2.0 37.0 88-89 30.343582987742607 35.0 33.0 35.0 2.0 36.0 90-91 30.138915178886407 35.0 33.0 35.0 2.0 36.0 92-93 29.994449286713568 35.0 33.0 35.0 2.0 36.0 94-95 29.789581392763296 35.0 33.0 35.0 2.0 36.0 96-97 29.56708801534829 35.0 32.0 35.0 2.0 35.0 98-99 29.439772070472713 35.0 32.0 35.0 2.0 35.0 100 29.365166165058266 35.0 32.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 2.7827576476390306 1101 2 2.2716603679754073 1101 3 2.0686174333324026 1101 4 1.0665566369452861 1101 5 1.6361466546930998 1101 6 1.0499244918980466 1101 7 0.5072301871979761 1101 8 0.39049756216793696 1101 9 0.4092658221302443 1101 10-11 0.31489032037122655 1101 12-13 0.2594079611094102 1101 14-15 0.3919978444779417 1101 16-17 0.34152811037852615 1101 18-19 0.3807014664709456 1101 20-21 0.42041122449774804 1101 22-23 0.4263716830827988 1101 24-25 0.4661539095626921 1101 26-27 0.4198942011958309 1101 28-29 0.5129542675549175 1101 30-31 0.4687180552053505 1101 32-33 0.42269153067271503 1101 34-35 0.4184364064328747 1101 36-37 0.44957685426044236 1101 38-39 0.43229716874201785 1101 40-41 0.3897752332119566 1101 42-43 0.4229180149152256 1101 44-45 0.4315568564595722 1101 46-47 0.448489829628663 1101 48-49 0.3634070721345779 1101 50-51 0.4164369634560927 1101 52-53 0.3375824265642251 1101 54-55 0.33431995485751287 1101 56-57 0.37300078044803797 1101 58-59 0.16799128656234075 1101 60-61 0.0986442891496182 1101 62-63 0.20095504610026182 1101 64-65 0.14867330448554839 1101 66-67 0.22890319780739787 1101 68-69 0.1764632569108855 1101 70-71 0.2842683297266717 1101 72-73 0.20550482876114273 1101 74-75 0.32557079704066183 1101 76-77 0.33413037632100995 1101 78-79 0.3171744164810413 1101 80-81 0.33873859456160815 1101 82-83 0.37993102963962144 1101 84-85 0.4416979435889239 1101 86-87 0.4003650985325784 1101 88-89 0.3970108710589173 1101 90-91 0.29416272195218696 1101 92-93 0.4110409351011661 1101 94-95 0.4025714256294357 1101 96-97 0.4296868323732248 1101 98-99 0.4367361077739318 1101 100 0.4146131058318403 1102 1 2.6831077636754017 1102 2 2.1921052105600225 1102 3 2.05684138353163 1102 4 1.0668896029517398 1102 5 1.5583990921867183 1102 6 1.060832093375133 1102 7 0.5597106471106912 1102 8 0.46310604522954435 1102 9 0.41594977262138855 1102 10-11 0.32505946710250555 1102 12-13 0.3515236909709003 1102 14-15 0.47504846078433616 1102 16-17 0.426733655311601 1102 18-19 0.4849873319271367 1102 20-21 0.5584429666488759 1102 22-23 0.5389563841614233 1102 24-25 0.5669992783248148 1102 26-27 0.4909527959150779 1102 28-29 0.595817875556321 1102 30-31 0.5898608948854118 1102 32-33 0.5839253020007789 1102 34-35 0.6280503665572255 1102 36-37 0.6308686346524865 1102 38-39 0.5321794447334298 1102 40-41 0.5542307927408032 1102 42-43 0.576436325308876 1102 44-45 0.5630398870523052 1102 46-47 0.5596767576569164 1102 48-49 0.5541539468152976 1102 50-51 0.5888280607660903 1102 52-53 0.474021633357097 1102 54-55 0.5044767590406636 1102 56-57 0.4316249192975974 1102 58-59 0.350580722143917 1102 60-61 0.2805490918877922 1102 62-63 0.31568623762595394 1102 64-65 0.3877748253648008 1102 66-67 0.3733958488371272 1102 68-69 0.4054250506580601 1102 70-71 0.4366732565288842 1102 72-73 0.4405425679493291 1102 74-75 0.49772357279125146 1102 76-77 0.38936487561311495 1102 78-79 0.44758009678988486 1102 80-81 0.5281624995139502 1102 82-83 0.588044818263679 1102 84-85 0.5767797437582338 1102 86-87 0.5578632649389519 1102 88-89 0.5533158332558727 1102 90-91 0.4080108693840607 1102 92-93 0.5020188790680038 1102 94-95 0.5684934039401206 1102 96-97 0.48290665926738896 1102 98-99 0.5251378983100317 1102 100 0.5371906641054522 1103 1 2.535724968389175 1103 2 2.125958999634186 1103 3 1.9559282315848527 1103 4 0.9440103401544633 1103 5 1.4532209218311891 1103 6 0.9026119489482198 1103 7 0.42272674943203725 1103 8 0.2911601911064139 1103 9 0.2854920061210393 1103 10-11 0.18251296547485651 1103 12-13 0.12325525024137818 1103 14-15 0.2648038430323396 1103 16-17 0.30941521955741536 1103 18-19 0.2901122839506698 1103 20-21 0.3352871284468222 1103 22-23 0.26561072798561014 1103 24-25 0.4322649435273149 1103 26-27 0.36537530149456643 1103 28-29 0.3858824394325282 1103 30-31 0.3984505913188059 1103 32-33 0.4226886376796273 1103 34-35 0.42607702369968337 1103 36-37 0.38373700501653474 1103 38-39 0.39526062623370706 1103 40-41 0.30960015817215947 1103 42-43 0.40026498893347906 1103 44-45 0.39686786656813666 1103 46-47 0.42517475330705423 1103 48-49 0.4490803917951851 1103 50-51 0.41464197251782764 1103 52-53 0.42472026833797827 1103 54-55 0.3066576000857637 1103 56-57 0.24870689329790707 1103 58-59 0.19390115514034534 1103 60-61 0.30991734852986497 1103 62-63 0.35292063461516676 1103 64-65 0.3888711763108219 1103 66-67 0.39521714185602264 1103 68-69 0.41943573163948145 1103 70-71 0.3143754861904213 1103 72-73 0.442328580501389 1103 74-75 0.3937277112051163 1103 76-77 0.45193817298187255 1103 78-79 0.36343804915459543 1103 80-81 0.37817298325881765 1103 82-83 0.47438680868260263 1103 84-85 0.5016794818423023 1103 86-87 0.48038194910459 1103 88-89 0.5448753080735642 1103 90-91 0.5265929073529882 1103 92-93 0.5101595513781234 1103 94-95 0.5615865706707552 1103 96-97 0.5479049889758336 1103 98-99 0.47598757029319927 1103 100 0.4522834363629755 1104 1 1.475747546281383 1104 2 1.6466882398759282 1104 3 1.7104819439465082 1104 4 0.8279670243725263 1104 5 1.4595519686524625 1104 6 0.9784078762237343 1104 7 0.42172009008534417 1104 8 0.3542041026696623 1104 9 0.2735687095921122 1104 10-11 0.2316132987033086 1104 12-13 0.17698999721709896 1104 14-15 0.3076053882340375 1104 16-17 0.3222846017944292 1104 18-19 0.3182687444352297 1104 20-21 0.38752564942950585 1104 22-23 0.3532451160564065 1104 24-25 0.3965015274591508 1104 26-27 0.3570253872496494 1104 28-29 0.4583300343952388 1104 30-31 0.4370933903942742 1104 32-33 0.28824823474171524 1104 34-35 0.25527513861106144 1104 36-37 0.2981290458743402 1104 38-39 0.32311913622338295 1104 40-41 0.2618038037532173 1104 42-43 0.296753371633109 1104 44-45 0.23712772883776978 1104 46-47 0.29715722323619076 1104 48-49 0.282869428200506 1104 50-51 0.23270626097695413 1104 52-53 0.26846726277575783 1104 54-55 0.21457076398046837 1104 56-57 0.1316031716448549 1104 58-59 0.25003197439839653 1104 60-61 0.1854130622989345 1104 62-63 0.21287517093063002 1104 64-65 0.2673864873247709 1104 66-67 0.3261149688426883 1104 68-69 0.2751500044533586 1104 70-71 0.18038126644416508 1104 72-73 0.20908128512659374 1104 74-75 0.2781771716291743 1104 76-77 0.32417147815812086 1104 78-79 0.3294803911887705 1104 80-81 0.25668600950148246 1104 82-83 0.32951724083512346 1104 84-85 0.3073784793281824 1104 86-87 0.23378323915173382 1104 88-89 0.31614758948949273 1104 90-91 0.32826491385653256 1104 92-93 0.3604320429930148 1104 94-95 0.3038850690755517 1104 96-97 0.3640542039066652 1104 98-99 0.3089793031203172 1104 100 0.2526385442194119 1105 1 2.153938739898482 1105 2 1.9180159031020096 1105 3 1.9129921908706358 1105 4 0.924944064271557 1105 5 1.5470678236244737 1105 6 1.0508669067437424 1105 7 0.4965415655558232 1105 8 0.35724779264160844 1105 9 0.26293242716445775 1105 10-11 0.21121977330837183 1105 12-13 0.15545099596601375 1105 14-15 0.2316346615997773 1105 16-17 0.23748263859221908 1105 18-19 0.1857810025686959 1105 20-21 0.231957577570455 1105 22-23 0.2574586180982905 1105 24-25 0.3577602181909185 1105 26-27 0.21811455346846742 1105 28-29 0.2610661978399804 1105 30-31 0.2788188717776805 1105 32-33 0.29621888597541357 1105 34-35 0.3095857637418078 1105 36-37 0.27712951760299376 1105 38-39 0.2853239771287548 1105 40-41 0.29243126410536746 1105 42-43 0.28777339322469686 1105 44-45 0.3098838206266379 1105 46-47 0.3402130491061257 1105 48-49 0.30268174102577206 1105 50-51 0.3477960388484149 1105 52-53 0.37801576027196404 1105 54-55 0.4849907693303095 1105 56-57 0.4649596144370065 1105 58-59 0.43025995968199027 1105 60-61 0.43119269078120936 1105 62-63 0.4099378601514232 1105 64-65 0.37257426690213435 1105 66-67 0.3985991064784784 1105 68-69 0.31554191517826524 1105 70-71 0.34417884891668393 1105 72-73 0.3723407146570139 1105 74-75 0.4141325203530215 1105 76-77 0.4054253122074236 1105 78-79 0.4859178662334571 1105 80-81 0.45876040640004945 1105 82-83 0.48493403638146404 1105 84-85 0.5017302336634799 1105 86-87 0.5026363334308286 1105 88-89 0.5141526799770162 1105 90-91 0.5447797304341364 1105 92-93 0.5170296650032995 1105 94-95 0.5632095245045861 1105 96-97 0.5364310277630615 1105 98-99 0.5199216600337806 1105 100 0.48822698056302727 1106 1 1.6678738940204099 1106 2 1.089134986692855 1106 3 1.5486407384035026 1106 4 0.7560701060317285 1106 5 1.4720973020204227 1106 6 0.9729150733016283 1106 7 0.4845725507098706 1106 8 0.3678140669222998 1106 9 0.34479280429521 1106 10-11 0.20624767445165304 1106 12-13 0.2134179730623842 1106 14-15 0.26644110328226134 1106 16-17 0.24403163510957881 1106 18-19 0.24175871411200234 1106 20-21 0.2910334495097118 1106 22-23 0.24794360047211228 1106 24-25 0.38137742150385634 1106 26-27 0.3477936814455376 1106 28-29 0.3407379467088987 1106 30-31 0.27892126641238946 1106 32-33 0.3029026336291949 1106 34-35 0.23409763954152396 1106 36-37 0.1707683206558599 1106 38-39 0.17491674671850888 1106 40-41 0.18285262385945344 1106 42-43 0.17627697095105077 1106 44-45 0.27531191286599466 1106 46-47 0.2942925294546228 1106 48-49 0.22026884366334087 1106 50-51 0.012040304064214524 1106 52-53 0.10093273440146788 1106 54-55 0.06757120541278283 1106 56-57 0.015786911355576194 1106 58-59 0.11741445386314098 1106 60-61 0.11374465009924961 1106 62-63 0.1361908758230257 1106 64-65 0.07793758527946437 1106 66-67 0.06844515444623056 1106 68-69 0.08758627807215191 1106 70-71 0.11240732537681453 1106 72-73 0.05767634743202166 1106 74-75 0.05212977968632515 1106 76-77 -0.13167605746662403 1106 78-79 -0.09322048215477707 1106 80-81 -0.06130019357469507 1106 82-83 0.02187613396522181 1106 84-85 0.02038053646048965 1106 86-87 -0.014380777478436357 1106 88-89 -0.011282091496315871 1106 90-91 0.011436532932183496 1106 92-93 0.037706357113940214 1106 94-95 0.027646534053811678 1106 96-97 0.019653902694020786 1106 98-99 -0.008135864551835681 1106 100 0.05445678374116625 1107 1 1.533687256402402 1107 2 0.9822623386437357 1107 3 1.3159709027924968 1107 4 0.6637135337731976 1107 5 1.3627786250546876 1107 6 0.941309804931656 1107 7 0.5101106110518501 1107 8 0.4085993709428948 1107 9 0.35951684205846135 1107 10-11 0.17070680827353613 1107 12-13 0.10915557786861285 1107 14-15 0.18425073555267346 1107 16-17 0.23232634693370358 1107 18-19 0.2216821312903221 1107 20-21 0.23656639332696727 1107 22-23 0.23604025327464484 1107 24-25 0.31681430669564037 1107 26-27 0.20996770930583608 1107 28-29 0.2093315626064367 1107 30-31 0.057525940605295034 1107 32-33 0.052904284121034095 1107 34-35 0.0696541853921886 1107 36-37 0.15346621462825993 1107 38-39 0.10784240856574456 1107 40-41 0.17567380957279255 1107 42-43 0.13088431894075114 1107 44-45 0.13403476227512812 1107 46-47 0.22436614139098054 1107 48-49 0.21294231035640365 1107 50-51 0.1927765884769599 1107 52-53 0.2321278555475743 1107 54-55 0.22721172828860148 1107 56-57 0.26996843244887003 1107 58-59 0.2645302193612622 1107 60-61 0.23939907011641992 1107 62-63 0.3128578725880615 1107 64-65 0.2726518851408244 1107 66-67 0.1869248864063522 1107 68-69 0.27387811804046436 1107 70-71 0.2029392788989739 1107 72-73 0.21866697263833856 1107 74-75 0.20586236699734428 1107 76-77 0.23110782713098743 1107 78-79 0.15910918708665633 1107 80-81 0.22999758816426308 1107 82-83 0.2826035057220082 1107 84-85 0.18033217704938664 1107 86-87 0.13350411722018407 1107 88-89 0.12567737242393306 1107 90-91 0.11569892812594773 1107 92-93 0.16269480367101607 1107 94-95 0.10749732959088121 1107 96-97 0.14942960085207346 1107 98-99 0.16156935760436753 1107 100 0.11004865011734566 1108 1 0.5734929953451235 1108 2 0.18315859311095295 1108 3 -0.05238805987096029 1108 4 -0.00263865590522272 1108 5 1.0261995566113455 1108 6 0.7732819884175441 1108 7 0.3580018433898644 1108 8 0.2895194967574497 1108 9 0.3015381540362654 1108 10-11 0.19547234127887236 1108 12-13 0.13403108378359008 1108 14-15 0.17245992718942205 1108 16-17 0.20430473483181544 1108 18-19 0.19231015876236768 1108 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-0.9095763107919055 2215 62-63 -0.8072387042579052 2215 64-65 -0.7945734213816138 2215 66-67 -0.9194230509309094 2215 68-69 -0.9569830939091659 2215 70-71 -0.8120693871039748 2215 72-73 -0.8930604623144873 2215 74-75 -0.8661367967221594 2215 76-77 -0.8428645607939735 2215 78-79 -0.8168182331644722 2215 80-81 -0.8059190651460924 2215 82-83 -0.6686679711271211 2215 84-85 -0.7085223380268673 2215 86-87 -0.7561814625665093 2215 88-89 -0.7412749145468212 2215 90-91 -0.7678913971869683 2215 92-93 -0.7802634181810006 2215 94-95 -0.8308323946139886 2215 96-97 -0.8873734922353655 2215 98-99 -0.8677356053664802 2215 100 -0.8819832872573521 2216 1 -5.683575812174965 2216 2 -4.637203086412207 2216 3 -5.592686318512548 2216 4 -2.7816590416511744 2216 5 -4.070452421248795 2216 6 -1.9217719014699952 2216 7 -1.1268261239696216 2216 8 -0.7465863048493375 2216 9 -0.5915477414818113 2216 10-11 -0.9339956026910698 2216 12-13 -0.6614775717210861 2216 14-15 -1.505097597992318 2216 16-17 -0.9849316397742243 2216 18-19 -1.5408692479814405 2216 20-21 -0.9937991754044191 2216 22-23 -0.8325073547260828 2216 24-25 -1.392700980405884 2216 26-27 -1.227353141698785 2216 28-29 -1.995638699550966 2216 30-31 -2.0098610627179383 2216 32-33 -1.2345931539880795 2216 34-35 -1.1506789610121686 2216 36-37 -1.1959590940455342 2216 38-39 -1.0698410699728456 2216 40-41 -1.219796196290737 2216 42-43 -1.4249286743411247 2216 44-45 -1.3814368058703366 2216 46-47 -1.728382479379178 2216 48-49 -1.3772127254304323 2216 50-51 -1.250260658377826 2216 52-53 -1.1928588006158662 2216 54-55 -1.1778474770620946 2216 56-57 -1.0597844619115335 2216 58-59 -1.061387120941255 2216 60-61 -1.0446339737704022 2216 62-63 -0.9170126329961263 2216 64-65 -1.0275659174965455 2216 66-67 -1.2027565034315018 2216 68-69 -1.1571764997966696 2216 70-71 -1.1521053985919707 2216 72-73 -1.1758966529957462 2216 74-75 -1.1295977286968792 2216 76-77 -1.131541841782596 2216 78-79 -1.0887425180953407 2216 80-81 -1.0604065482888565 2216 82-83 -1.1776313636142248 2216 84-85 -1.0949485255101585 2216 86-87 -1.1596357938753883 2216 88-89 -1.1450212913692255 2216 90-91 -1.1564648071926875 2216 92-93 -1.221903799894129 2216 94-95 -1.2622651490705081 2216 96-97 -1.193792814655822 2216 98-99 -1.1694671279130375 2216 100 -1.2849132997781467 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5593.0 3 4586.0 4 4918.0 5 6595.0 6 5692.0 7 4046.0 8 3668.0 9 3910.0 10 4145.0 11 7667.0 12 5283.0 13 6850.0 14 6304.0 15 6425.0 16 7640.0 17 9487.0 18 12619.0 19 19966.0 20 40273.0 21 54960.0 22 26205.0 23 17463.0 24 17864.0 25 19741.0 26 20210.0 27 22278.0 28 25747.0 29 30827.0 30 35710.0 31 44075.0 32 56985.0 33 78493.0 34 116450.0 35 263063.0 36 340134.0 37 569105.0 38 667954.0 39 231530.0 40 1845.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 14.96969884967228 43.3206438241239 25.333344742838666 16.376312583365156 2 17.63833925146931 41.615027096956396 23.526640883134704 17.21999276843959 3 18.48479816527492 24.994066933541816 20.411102709398047 36.110032191785216 4 34.13601367776714 26.11792869344968 21.883287139748838 17.86277048903434 5 17.25556657043102 40.52412673457563 22.901743430687887 19.31856326430546 6 36.722153606912435 23.64496245241966 22.914144074095983 16.718739866571926 7 18.538320482513313 23.914106302021235 39.63993947915873 17.90763373630673 8 35.7618876915062 24.33640522451935 22.324365197523008 17.577341886451443 9 35.63367644155698 25.691638759279993 22.28580917405301 16.388875625110018 10-11 16.675088176414118 43.713835910980485 22.43992636583466 17.171149546770735 12-13 16.62881738484684 25.15486550647007 31.44744372139033 26.768873387292764 14-15 16.885810029269795 34.197803090610925 22.209837416162028 26.706549463957245 16-17 26.40171912950272 24.887556729420616 31.265126657696385 17.445597483380276 18-19 17.328937043928924 25.13674560080048 31.288017058724172 26.246300296546423 20-21 26.63647505780301 34.34369152105523 22.187661077394296 16.832172343747466 22-23 26.121569617519715 25.33959733122047 31.356163975966233 17.182669075293585 24-25 17.028880670889063 34.33135944547743 22.121179942600698 26.518579941032804 26-27 17.446205220608974 29.765414155970426 35.577775057652694 17.210605565767903 28-29 27.08290312286918 25.146016178348468 30.65656456773848 17.114516131043874 30-31 24.010331622562223 27.091982515496365 31.150650964919098 17.747034897022317 32-33 19.4550660103023 28.466237971340213 32.01832399025538 20.060372028102105 34-35 20.31683297449044 28.253461390713987 32.17573537848838 19.25397025630719 36-37 20.409181823446346 27.379509726621144 33.34580621595015 18.86550223398237 38-39 21.912225437203155 27.346352996353122 32.16616339286255 18.575258173581172 40-41 18.874797392296088 30.104230828371296 32.189132476440314 18.831839302892302 42-43 18.990790198377706 27.42766482786614 33.47403937832993 20.10750559542623 44-45 18.927318106402264 28.7836355628282 31.961428323004032 20.32761800776551 46-47 20.43378366941501 27.339275453342776 33.1787847607339 19.048156116508313 48-49 19.143776275359983 27.61188325566354 33.1182454674308 20.126095001545675 50-51 20.442649030412273 28.976353848379816 31.781911007619996 18.79908611358791 52-53 20.57171049712771 27.824748000907274 32.49822135581062 19.105320146154394 54-55 19.543171582548528 29.633499942895558 30.126094806203913 20.697233668352006 56-57 19.852769948635 30.217135551102466 30.123916340034178 19.806178160228356 58-59 22.72414256964965 29.48275785270701 28.267927886675498 19.525171690967838 60-61 21.246970906447338 30.781898136929282 28.144234744059414 19.82689621256397 62-63 19.989231213019043 30.739791077692953 27.832966920189286 21.438010789098723 64-65 20.9637643973087 30.76858108108108 28.327631428897256 19.940023092712966 66-67 21.110659302945926 29.43691556228081 29.67640160812029 19.77602352665298 68-69 22.15491524934457 29.439517542351272 28.80670528318852 19.598861925115642 70-71 19.332303767253205 31.868865568926136 28.945150927295494 19.85367973652517 72-73 19.316647266127585 29.448183099276655 30.11009397605689 21.125075658538872 74-75 19.47632801730274 30.686637965363516 28.534200584728325 21.30283343260542 76-77 20.980491132891963 29.388666572469997 29.507635899337377 20.123206395300667 78-79 20.06696377259546 29.80410004756142 28.943646398206884 21.18528978163623 80-81 21.316973195041566 31.13733264719634 27.71121520373137 19.834478954030722 82-83 21.17214475070747 30.23992906934519 28.62453187157646 19.96339430837088 84-85 20.172754820755024 31.355741189915737 27.30839700396287 21.163106985366372 86-87 20.14484567494271 31.4951943807932 28.285817632363898 20.07414231190019 88-89 22.795326897316535 30.231930373848925 27.1394600573661 19.83328267146844 90-91 21.39716072713381 31.52706866832422 27.09482524220985 19.980945362332115 92-93 20.157811325622976 31.48382631702179 27.017910491377613 21.340451865977624 94-95 21.161135312665714 31.7931629499541 26.82634822299135 20.21935351438884 96-97 21.409142333161853 30.516799682678897 27.897599795865307 20.17645818829394 98-99 22.56512903222358 30.701915055642125 26.56876450563513 20.164191406499164 100 20.096628135621486 33.04307357008818 26.58399798732958 20.276300306960746 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 106304.0 1 57438.5 2 6151.5 3 3071.0 4 2285.0 5 2040.0 6 2108.0 7 2520.0 8 2346.5 9 1973.5 10 2067.5 11 3078.0 12 27366.5 13 30565.0 14 8993.0 15 7947.0 16 8857.0 17 9929.0 18 10767.5 19 11543.5 20 12421.0 21 13489.5 22 15012.5 23 17109.0 24 19474.0 25 22258.5 26 25668.0 27 29159.5 28 32531.5 29 35742.5 30 38986.0 31 42211.0 32 45641.5 33 49230.0 34 52150.0 35 55330.5 36 58639.0 37 61305.0 38 64514.5 39 66730.5 40 68348.5 41 69575.5 42 70425.0 43 72132.0 44 73577.5 45 74281.0 46 76314.0 47 82623.0 48 87199.0 49 102924.0 50 163501.5 51 162360.0 52 96303.0 53 73786.5 54 69591.5 55 67172.5 56 63213.0 57 57631.0 58 51692.5 59 43412.5 60 34538.0 61 27928.5 62 23539.5 63 19299.0 64 14928.0 65 11538.0 66 8960.0 67 7268.0 68 5902.0 69 4366.5 70 3041.5 71 2221.5 72 1680.5 73 1129.5 74 718.5 75 484.5 76 334.0 77 213.5 78 123.5 79 81.0 80 55.5 81 31.0 82 23.5 83 17.5 84 6.0 85 2.5 86 2.5 87 2.5 88 2.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05797657133612657 2 0.06884495133460143 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 7.126806556376959E-5 18-19 0.0 20-21 2.67255245864136E-4 22-23 1.603531475184816E-4 24-25 0.0 26-27 0.0022271270488677998 28-29 8.730338031561775E-4 30-31 0.005843981376229107 32-33 0.0014075442948844495 34-35 0.002280578098040627 36-37 0.007786036162841828 38-39 0.012293741309750254 40-41 0.002939807704505496 42-43 0.007821670195623713 44-45 0.0017817016390942399 46-47 0.0024409312455591086 48-49 0.0031536119011968044 50-51 0.009549920785545127 52-53 0.0034030501306699977 54-55 0.0015857144587938736 56-57 6.235955736829839E-4 58-59 0.0010155699342837168 60-61 0.006627930097430572 62-63 0.0016926165571395277 64-65 0.008053291408705966 66-67 0.010583307736219785 68-69 0.012097754129449889 70-71 0.009603371834717954 72-73 0.0040088286879620405 74-75 0.001621348491575758 76-77 0.004293900950217118 78-79 0.01640947209605795 80-81 0.009193580457726279 82-83 0.0065922960646486875 84-85 0.004721509343599736 86-87 0.007696951080887117 88-89 0.004240449901044291 90-91 0.005879615409010992 92-93 0.010583307736219785 94-95 0.008623435933216121 96-97 0.011242537342684654 98-99 0.002458748261950051 100 0.002993258753678323 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2806306.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.36361558311466 #Duplication Level Percentage of deduplicated Percentage of total 1 83.26356393440916 47.76299073613537 2 11.497368302121028 13.190612310007166 3 2.6562101398268654 4.571094521069987 4 0.8973543950296292 2.0590197023319226 5 0.4268206721008544 1.2241988478660024 6 0.2612885513630117 0.8993073609994028 7 0.1927157096584695 0.7738408919972907 8 0.1277406791443765 0.5862133770211206 9 0.09937987223906962 0.5130709909038934 >10 0.505704225661911 6.053422707067199 >50 0.05029861140457422 1.8082795930785 >100 0.015357690743119577 1.673350551970584 >500 0.0015649536106031245 0.6437686444901435 >1k 0.004319271965263212 5.669351009483022 >5k 1.2519628884820905E-4 0.4406339266251982 >10k+ 1.8779443327231356E-4 12.130844828953158 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 180676 6.4382145068998184 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 102853 3.6650671737151974 No Hit AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG 54297 1.9348210779579988 No Hit TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 7180 0.2558523553739328 No Hit GTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGT 5091 0.1814128608925755 No Hit TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4493 0.16010370928900838 No Hit AGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTC 3745 0.13344945276815856 No Hit ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 3641 0.12974351335884254 No Hit TTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTG 3509 0.12503982103163375 No Hit CCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATT 3401 0.1211913454911902 No Hit CCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGAT 3393 0.12090627322893513 No Hit CACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCA 3369 0.1200510564421699 No Hit TCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGT 3308 0.11787738044247492 No Hit TACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACT 3282 0.1169508955901459 No Hit ACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGA 3281 0.11691526155736401 No Hit AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 3206 0.11424270909872265 No Hit AACGAGTGGAATCACTGCTGAGTACATGGTAACGAGTGGAATCACTGCTG 3167 0.11285298182022915 No Hit TCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCA 3148 0.11217593519737336 No Hit ACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTC 3147 0.11214030116459146 No Hit TCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGA 3114 0.11096437808278926 No Hit CTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCG 3085 0.1099309911321146 No Hit TGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCA 3078 0.10968155290264142 No Hit CCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTC 3069 0.10936084660760445 No Hit CGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAG 3000 0.1069020983456544 No Hit TCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACT 2928 0.1043364479853587 No Hit ATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTA 2840 0.10120065310055283 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.06214575317160709 0.0 0.0 0.0 0.0 2 0.06731268792498038 0.0 0.0 0.0 0.0 3 0.07087609120316886 0.0 0.0 0.0 0.0 4 0.07130369959655149 0.0 0.0 0.0 0.0 5 0.07141060169489713 3.5634032781884796E-5 0.0 0.0 0.0 6 0.07141060169489713 7.126806556376959E-5 0.0 0.0 0.0 7 0.0714818697604609 7.126806556376959E-5 0.0 0.0 0.0 8 0.0719807462194073 1.4253613112753918E-4 0.0 0.0 0.0 9 0.0726577928422631 1.4253613112753918E-4 0.0 0.0 0.0 10-11 0.07303195018647289 1.603531475184816E-4 0.0 0.0 0.0 12-13 0.07319230333399138 2.1380419669130878E-4 0.0 0.0 0.0 14-15 0.07331702244872798 2.494382294731936E-4 0.0 0.0 0.0 16-17 0.0734595585798555 3.207062950369632E-4 0.0 0.0 0.0 18-19 0.07358427769459211 3.9197436060073277E-4 0.0 3.5634032781884796E-5 0.0 20-21 0.07370899680932871 3.9197436060073277E-4 0.0 3.5634032781884796E-5 0.0 22-23 0.07392280100602001 4.0979137699167513E-4 0.0 3.5634032781884796E-5 0.0 24-25 0.07406533713714755 4.810594425554448E-4 0.0 3.5634032781884796E-5 0.0 26-27 0.07424350730105697 4.988764589463872E-4 0.0 3.5634032781884796E-5 0.0 28-29 0.07447512851413923 4.988764589463872E-4 0.0 3.5634032781884796E-5 0.0 30-31 0.07483146884195807 4.988764589463872E-4 0.0 3.5634032781884796E-5 0.0 32-33 0.07499182198947656 5.34510491728272E-4 0.0 3.5634032781884796E-5 0.0 34-35 0.07541943038285917 5.34510491728272E-4 0.0 3.5634032781884796E-5 0.0 36-37 0.07593612385819651 5.879615409010992E-4 0.0 3.5634032781884796E-5 0.0 38-39 0.07650626838270666 6.057785572920416E-4 0.0 3.5634032781884796E-5 0.0 40-41 0.07745057025142661 6.235955736829839E-4 0.0 3.5634032781884796E-5 0.0 42-43 0.07848395720210127 6.414125900739264E-4 0.0 3.5634032781884796E-5 0.0 44-45 0.0794638931036031 6.770466228558111E-4 0.0 3.5634032781884796E-5 0.0 46-47 0.08053291408705965 6.948636392467536E-4 0.0 3.5634032781884796E-5 0.0 48-49 0.08183355628359845 7.483146884195807E-4 0.0 3.5634032781884796E-5 0.0 50-51 0.08327673461126478 7.483146884195807E-4 0.0 3.5634032781884796E-5 0.0 52-53 0.0849693511684043 7.661317048105231E-4 0.0 3.5634032781884796E-5 0.0 54-55 0.08723211225005398 8.195827539833504E-4 0.0 3.5634032781884796E-5 0.0 56-57 0.09031445608568703 8.195827539833504E-4 0.0 3.5634032781884796E-5 0.0 58-59 0.09371750621635702 8.908508195471199E-4 0.0 3.5634032781884796E-5 0.0 60-61 0.09895570903529408 9.264848523290048E-4 0.0 3.5634032781884796E-5 0.0 62-63 0.10593997946054351 9.264848523290048E-4 0.0 3.5634032781884796E-5 0.0 64-65 0.11427834313150455 9.264848523290048E-4 0.0 3.5634032781884796E-5 0.0 66-67 0.12511108909719754 9.621188851108895E-4 0.0 3.5634032781884796E-5 0.0 68-69 0.13836694929205867 9.977529178927743E-4 0.0 3.5634032781884796E-5 0.0 70-71 0.1575380589287127 0.001033386950674659 0.0 3.5634032781884796E-5 0.0 72-73 0.18185828630234907 0.001069020983456544 0.0 3.5634032781884796E-5 0.0 74-75 0.21332313724875335 0.0011402890490203135 0.0 3.5634032781884796E-5 0.0 76-77 0.25417755583318424 0.0011759230818021984 0.0 3.5634032781884796E-5 0.0 78-79 0.30212314694121023 0.0011759230818021984 0.0 3.5634032781884796E-5 0.0 80-81 0.36711962273536813 0.0011759230818021984 0.0 3.5634032781884796E-5 0.0 82-83 0.4463875286586709 0.0011937400981931407 0.0 3.5634032781884796E-5 0.0 84-85 0.5461093693987755 0.0012293741309750256 0.0 3.5634032781884796E-5 0.0 86-87 0.6683519188570313 0.0012650081637569104 0.0 3.5634032781884796E-5 0.0 88 0.7764655743172697 0.0012828251801478528 0.0 3.5634032781884796E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGAGT 2225 0.0 85.444954 1 AACGTGT 1355 0.0 62.85999 1 CGTGTGG 1710 0.0 61.287346 3 ATCGAGT 1030 0.0 57.109432 1 ACGTGTG 1855 0.0 55.26638 2 GTGGAAT 94190 0.0 51.696236 6 TGGAATC 95040 0.0 51.461353 7 GAATCAC 95435 0.0 51.258205 9 GGAATCA 95525 0.0 51.21483 8 TCGAGTG 1250 0.0 51.165516 2 AGTGGAA 93640 0.0 50.785324 5 ACGAGTG 95270 0.0 48.784412 2 CGAGTGG 97100 0.0 48.564278 3 AACGAGT 91825 0.0 48.40342 1 GAGTGGA 99200 0.0 48.005215 4 AGTACTT 28760 0.0 40.63175 20-21 GAGTACT 28520 0.0 40.471077 20-21 GTACTTT 28325 0.0 39.86238 22-23 TACTTTT 29815 0.0 38.532307 22-23 CATGGGG 15430 0.0 37.403305 24-25 >>END_MODULE