FastQCFastQC Report
Thu 26 May 2016
SRR2132677_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132677_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4221331
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3125947.405105167066975No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1696614.0191351969319635No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG317030.7510190506264492No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT124520.2949780531306358No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA73890.17503957874897752No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT60510.14334341467181796No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT57470.13614189458253806No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA55740.1320436611106781No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT31650.086.513711
AACGAGT917100.069.4501951
GTGGAAT1010600.069.399456
ACGAGTG968600.068.906252
GAATCAC1025900.068.8774649
TGGAATC1029300.068.544947
AGTGGAA1003350.068.3787845
GGAATCA1034100.068.294948
CGAGTGG993350.068.215643
GAGTGGA1024050.067.060834
ATCGAGT12400.065.638721
CGTGTGG23150.060.490373
AACGTGT20100.060.1551441
ACGATTG5750.058.0533872
TCGAGTG17300.055.9832572
ACGTGTG23800.054.9167562
CGATTGG7500.050.124463
GACGAGT8950.049.9385871
GAGTACT469450.041.71217720-21
AGTACTT478200.041.70561220-21
Produced by FastQC (version 0.11.5)