##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132677_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4221331
Sequences flagged as poor quality	0
Sequence length	100
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.221503833743434	31.0	28.0	34.0	16.0	34.0
2	30.33652158525356	33.0	30.0	34.0	23.0	34.0
3	30.785918706682796	33.0	31.0	34.0	25.0	34.0
4	35.21644405520439	37.0	35.0	37.0	32.0	37.0
5	34.67308818000768	37.0	35.0	37.0	32.0	37.0
6	35.20543402069158	37.0	35.0	37.0	32.0	37.0
7	35.59606460616332	37.0	35.0	37.0	33.0	37.0
8	35.79697375069617	37.0	35.0	37.0	35.0	37.0
9	37.70464978936738	39.0	39.0	39.0	35.0	39.0
10-11	37.79168418681217	39.0	39.0	39.0	35.0	39.0
12-13	37.78532529195176	39.0	39.0	39.0	35.0	39.0
14-15	39.206715133212725	41.0	39.0	41.0	36.0	41.0
16-17	39.12931075530443	41.0	39.0	41.0	36.0	41.0
18-19	38.96185847544294	41.0	39.0	41.0	35.5	41.0
20-21	38.79716361972089	40.5	39.0	41.0	35.0	41.0
22-23	38.696879135988155	40.5	39.0	41.0	35.0	41.0
24-25	38.61733135828486	40.5	39.0	41.0	35.0	41.0
26-27	38.562755680613535	40.5	38.5	41.0	35.0	41.0
28-29	38.38864696940372	41.0	38.0	41.0	35.0	41.0
30-31	38.26042165373907	40.5	38.0	41.0	34.5	41.0
32-33	38.12841779050257	40.0	38.0	41.0	34.0	41.0
34-35	38.05116383908298	40.0	38.0	41.0	34.0	41.0
36-37	37.89311238564329	40.0	38.0	41.0	34.0	41.0
38-39	37.443437034432975	40.0	36.0	41.0	33.5	41.0
40-41	37.24483131031421	40.0	35.0	41.0	33.0	41.0
42-43	37.10972617404321	40.0	35.0	41.0	33.0	41.0
44-45	36.97010765088073	40.0	35.0	41.0	33.0	41.0
46-47	36.8358755804745	40.0	35.0	41.0	33.0	41.0
48-49	36.68531583995664	40.0	35.0	41.0	32.5	41.0
50-51	36.42924115640304	39.0	35.0	41.0	31.5	41.0
52-53	35.953770505084776	39.0	35.0	41.0	30.5	41.0
54-55	35.13139469518026	39.0	35.0	41.0	25.5	41.0
56-57	34.48746023469849	39.0	35.0	41.0	20.0	41.0
58-59	34.17745931318819	39.0	35.0	41.0	16.5	41.0
60-61	33.93216132542082	38.0	35.0	40.5	13.5	41.0
62-63	33.6464398314181	38.0	34.0	40.0	9.0	41.0
64-65	33.38086897236914	37.0	34.0	40.0	7.5	41.0
66-67	33.06351645961901	36.5	34.0	40.0	6.5	41.0
68-69	32.76730526461914	36.0	34.0	39.5	2.0	41.0
70-71	32.43334768109868	36.0	33.5	39.0	2.0	41.0
72-73	32.08937181187639	35.0	33.0	39.0	2.0	40.5
74-75	31.73669738288706	35.0	33.0	38.0	2.0	40.0
76-77	31.37186790137992	35.0	33.0	37.0	2.0	39.5
78-79	31.233464160948287	35.0	33.0	37.0	2.0	39.0
80-81	31.074367065743008	35.0	33.0	36.5	2.0	39.0
82-83	30.753009773457705	35.0	33.0	36.0	2.0	38.0
84-85	30.45759891844539	35.0	33.0	36.0	2.0	37.0
86-87	30.264076662076487	35.0	33.0	35.0	2.0	37.0
88-89	30.049564580460522	35.0	33.0	35.0	2.0	36.5
90-91	29.843079351038806	35.0	33.0	35.0	2.0	36.0
92-93	29.67650523022241	35.0	33.0	35.0	2.0	36.0
94-95	29.49491380799089	35.0	32.5	35.0	2.0	36.0
96-97	29.30935835166681	35.0	32.0	35.0	2.0	35.5
98-99	29.157252061020564	35.0	32.0	35.0	2.0	35.0
100	29.050004607551504	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	2.807560951313512
1101	2	2.2499881706017852
1101	3	2.059036392756756
1101	4	1.06872370565101
1101	5	1.5992382256717192
1101	6	1.0196380934419693
1101	7	0.544938863293865
1101	8	0.40448202162592395
1101	9	0.36891170177550947
1101	10-11	0.291360712582275
1101	12-13	0.2546639054578179
1101	14-15	0.42936962879099383
1101	16-17	0.38033821281310054
1101	18-19	0.3948805505664694
1101	20-21	0.4496930078011445
1101	22-23	0.4228785080750228
1101	24-25	0.4690547261083964
1101	26-27	0.4189122021598237
1101	28-29	0.4422681196135869
1101	30-31	0.5072320628970601
1101	32-33	0.4536609247035628
1101	34-35	0.453921052578373
1101	36-37	0.4114264243673489
1101	38-39	0.3832993676792569
1101	40-41	0.3535219441082873
1101	42-43	0.33135034814712583
1101	44-45	0.3782985558065448
1101	46-47	0.46241663670065947
1101	48-49	0.35567187740809203
1101	50-51	0.3892791189636853
1101	52-53	0.3367723941835621
1101	54-55	0.33669651188672134
1101	56-57	0.3586270604990034
1101	58-59	0.21895838389703925
1101	60-61	0.24088753801925122
1101	62-63	0.23445886554799955
1101	64-65	0.19833296691478353
1101	66-67	0.2541857262148426
1101	68-69	0.24517211302431008
1101	70-71	0.2412598773770469
1101	72-73	0.28915427407361705
1101	74-75	0.23190293754760916
1101	76-77	0.18005047392501083
1101	78-79	0.15870433304310438
1101	80-81	0.22487316737587904
1101	82-83	0.31095317157310376
1101	84-85	0.3065039332333832
1101	86-87	0.3318917209991632
1101	88-89	0.3233184664265707
1101	90-91	0.27496568309634384
1101	92-93	0.32456092572841655
1101	94-95	0.32348058120382106
1101	96-97	0.35228198501433994
1101	98-99	0.3267171956050703
1101	100	0.33945411575870565
1102	1	2.7592447528348103
1102	2	2.212975479059825
1102	3	2.05462671804764
1102	4	1.039748887307283
1102	5	1.5946887720848295
1102	6	1.0355661329047834
1102	7	0.5030736013286372
1102	8	0.4522013650468466
1102	9	0.44067443772208037
1102	10-11	0.3697520035053614
1102	12-13	0.35547935227179295
1102	14-15	0.4887795908608723
1102	16-17	0.46505219081592486
1102	18-19	0.47321774563830843
1102	20-21	0.5173958413583435
1102	22-23	0.4541711073073955
1102	24-25	0.5236099794307947
1102	26-27	0.41507572338907295
1102	28-29	0.45207254873078995
1102	30-31	0.445064387866708
1102	32-33	0.4441570489346489
1102	34-35	0.4486930374399378
1102	36-37	0.43655537282052137
1102	38-39	0.4751998282117711
1102	40-41	0.45023791077763775
1102	42-43	0.4842400294747904
1102	44-45	0.47676838600259686
1102	46-47	0.4193211255358804
1102	48-49	0.46231833428489466
1102	50-51	0.4762656229116331
1102	52-53	0.4778160969788061
1102	54-55	0.49683252381252174
1102	56-57	0.4900963978550905
1102	58-59	0.489767617316069
1102	60-61	0.43668379518657474
1102	62-63	0.43952623376507916
1102	64-65	0.4652779608627
1102	66-67	0.439636045540432
1102	68-69	0.4635191123752165
1102	70-71	0.4541020423895503
1102	72-73	0.4711047068679335
1102	74-75	0.47459348261820367
1102	76-77	0.34521064741387875
1102	78-79	0.3884428357489682
1102	80-81	0.47481389452915934
1102	82-83	0.5114497654852315
1102	84-85	0.4688317274816747
1102	86-87	0.4540855080944013
1102	88-89	0.46008969739754946
1102	90-91	0.38834292185302033
1102	92-93	0.3910116391559093
1102	94-95	0.4391181036868623
1102	96-97	0.3876738267997588
1102	98-99	0.37881626469124896
1102	100	0.37542910731920287
1103	1	2.4402589367210226
1103	2	2.084198343289845
1103	3	1.9942894588650013
1103	4	1.0067828344865148
1103	5	1.5424767523297973
1103	6	1.0143588658999505
1103	7	0.5287052571901114
1103	8	0.42996303705179884
1103	9	0.4177107636338988
1103	10-11	0.3054185859623999
1103	12-13	0.2539798810432501
1103	14-15	0.4484049232561915
1103	16-17	0.43361868232803147
1103	18-19	0.4407293815054345
1103	20-21	0.45747046911551337
1103	22-23	0.4430536656129149
1103	24-25	0.538141526562427
1103	26-27	0.5440970841731385
1103	28-29	0.5520346385200412
1103	30-31	0.5258991175480787
1103	32-33	0.5634587085531848
1103	34-35	0.5082535013067115
1103	36-37	0.5317359951377227
1103	38-39	0.5017177005992792
1103	40-41	0.44087511721871664
1103	42-43	0.472907006742318
1103	44-45	0.4051940156014666
1103	46-47	0.4120559138561646
1103	48-49	0.36638126670052173
1103	50-51	0.32147543276864354
1103	52-53	0.3316836188458936
1103	54-55	0.3112193999317512
1103	56-57	0.29760685082982263
1103	58-59	0.27713212062109704
1103	60-61	0.2415140636877524
1103	62-63	0.185446091755459
1103	64-65	0.21889992140398107
1103	66-67	0.2888417694594523
1103	68-69	0.22317298125538088
1103	70-71	0.21531793019291
1103	72-73	0.2838256273507227
1103	74-75	0.24993848495773108
1103	76-77	0.23323162179131174
1103	78-79	0.2616619347069573
1103	80-81	0.2810552788914258
1103	82-83	0.31945090381760366
1103	84-85	0.3316297204147638
1103	86-87	0.279172991927652
1103	88-89	0.2626631259554273
1103	90-91	0.2771958785330959
1103	92-93	0.24747193556352798
1103	94-95	0.2593774102124158
1103	96-97	0.25321449571245225
1103	98-99	0.25480821046933855
1103	100	0.25189524831943544
1104	1	1.590055278387716
1104	2	1.7678245757378726
1104	3	1.8298702604713135
1104	4	0.9056001415048058
1104	5	1.542891078836682
1104	6	1.0118007523636834
1104	7	0.5106004559659425
1104	8	0.4003005823778736
1104	9	0.31858155697663193
1104	10-11	0.2742238165464599
1104	12-13	0.26528569904810695
1104	14-15	0.41755470470315004
1104	16-17	0.37635573470837613
1104	18-19	0.36824858947190364
1104	20-21	0.42097999489551796
1104	22-23	0.3925617885641728
1104	24-25	0.3967470235586603
1104	26-27	0.3486074501776457
1104	28-29	0.45475607033682053
1104	30-31	0.4462630406257446
1104	32-33	0.37482989594840177
1104	34-35	0.2907349780668014
1104	36-37	0.3023065291928688
1104	38-39	0.3416131043149164
1104	40-41	0.2932804798325961
1104	42-43	0.30845799501025795
1104	44-45	0.3028546392056555
1104	46-47	0.3949336378557078
1104	48-49	0.34821561948904645
1104	50-51	0.3109881905344807
1104	52-53	0.2873002127157278
1104	54-55	0.26914050269725465
1104	56-57	0.24610196579359922
1104	58-59	0.22928434241052997
1104	60-61	0.12915787180403981
1104	62-63	0.11997852297749745
1104	64-65	0.14696797452078414
1104	66-67	0.21623853234773094
1104	68-69	0.2579036436833775
1104	70-71	0.11903431941941278
1104	72-73	0.13071906075613526
1104	74-75	0.18861994308866414
1104	76-77	0.2156451829537005
1104	78-79	0.23557766638557354
1104	80-81	0.19689774741097565
1104	82-83	0.22740079904775
1104	84-85	0.21646110506669203
1104	86-87	0.1329658223624861
1104	88-89	0.21814710187179998
1104	90-91	0.15637101303261147
1104	92-93	0.1981478830801926
1104	94-95	0.2154891278441866
1104	96-97	0.2587604819381646
1104	98-99	0.3230401714391604
1104	100	0.3009363251227093
1105	1	2.159855678271704
1105	2	1.9861189112406912
1105	3	1.9352443308274552
1105	4	1.0032433656427102
1105	5	1.5468582668602977
1105	6	1.0337077752041068
1105	7	0.5393046425003263
1105	8	0.388932027896125
1105	9	0.30896437338615357
1105	10-11	0.25444394809792215
1105	12-13	0.21777228505170854
1105	14-15	0.29880984225813023
1105	16-17	0.2626278778521183
1105	18-19	0.22622135160179369
1105	20-21	0.3718069252261387
1105	22-23	0.3287111845304693
1105	24-25	0.41588296007012815
1105	26-27	0.29079117214932637
1105	28-29	0.3241727127088936
1105	30-31	0.3716945764597668
1105	32-33	0.367855406773522
1105	34-35	0.3112208141940158
1105	36-37	0.3586741482075695
1105	38-39	0.28187273211173647
1105	40-41	0.19783489884368777
1105	42-43	0.2845742814540273
1105	44-45	0.33687323659115265
1105	46-47	0.3864919869554839
1105	48-49	0.2879737956213333
1105	50-51	0.3460821523764466
1105	52-53	0.29994099299293
1105	54-55	0.2573100848402774
1105	56-57	0.17983112253580202
1105	58-59	0.20530867883002202
1105	60-61	0.32238911024219163
1105	62-63	0.3328615666792061
1105	64-65	0.2240236915393723
1105	66-67	0.18207689115531878
1105	68-69	0.1454657169939395
1105	70-71	0.1724121538094323
1105	72-73	0.21224304177329145
1105	74-75	0.2499165154472287
1105	76-77	0.18777211568873753
1105	78-79	0.20482728656340754
1105	80-81	0.25233407892246973
1105	82-83	0.3119239569290393
1105	84-85	0.25301274247215133
1105	86-87	0.25623915306098155
1105	88-89	0.2350596604981554
1105	90-91	0.24015147957895877
1105	92-93	0.2567423081474054
1105	94-95	0.2788927552022038
1105	96-97	0.2472839167026777
1105	98-99	0.23073521444058898
1105	100	0.2224207723255489
1106	1	1.649288876544368
1106	2	1.1438920543494895
1106	3	1.5781671416700966
1106	4	0.8526830697297072
1106	5	1.5376431746576813
1106	6	1.0245324080791391
1106	7	0.6096045661473042
1106	8	0.40430331239513606
1106	9	0.36192640292084377
1106	10-11	0.21736167946099982
1106	12-13	0.15933808586332532
1106	14-15	0.27770525718001693
1106	16-17	0.27217082256803593
1106	18-19	0.23776088928919137
1106	20-21	0.3303262702590999
1106	22-23	0.2941915165503275
1106	24-25	0.3783172815814311
1106	26-27	0.3144041121304113
1106	28-29	0.4126117754047556
1106	30-31	0.3624866986437212
1106	32-33	0.36407056496250334
1106	34-35	0.3939649917704884
1106	36-37	0.37127012252472014
1106	38-39	0.3282355738485023
1106	40-41	0.3606629489484803
1106	42-43	0.34337677048172566
1106	44-45	0.4229486571669199
1106	46-47	0.4365454699142717
1106	48-49	0.3501918601512273
1106	50-51	0.23421255420582554
1106	52-53	0.301603162385625
1106	54-55	0.28045120623717423
1106	56-57	0.25309872086008056
1106	58-59	0.299247780768475
1106	60-61	0.296439513163385
1106	62-63	0.2853222859526312
1106	64-65	0.3255193349689094
1106	66-67	0.3040545226970295
1106	68-69	0.2666011148549501
1106	70-71	0.31761577918113915
1106	72-73	0.3681561597888958
1106	74-75	0.3476826656557179
1106	76-77	0.18008288922169768
1106	78-79	0.16506838860552975
1106	80-81	0.18161757040250492
1106	82-83	0.13011632620343505
1106	84-85	0.14653941355383893
1106	86-87	0.15013615882403997
1106	88-89	0.11597840987091956
1106	90-91	0.13810972546657396
1106	92-93	0.13983248448723273
1106	94-95	0.1323233017053127
1106	96-97	0.146395683266082
1106	98-99	0.1831834234162777
1106	100	0.20894002472026685
1107	1	1.587779658152698
1107	2	1.083318800548298
1107	3	1.403930664958306
1107	4	0.7032538852417431
1107	5	1.4409892178502517
1107	6	0.9733665452554945
1107	7	0.5422887142257977
1107	8	0.4336582052813185
1107	9	0.43419458061497807
1107	10-11	0.2299090308551328
1107	12-13	0.1888093195913001
1107	14-15	0.2718035699927057
1107	16-17	0.26762194601766254
1107	18-19	0.25045218099755573
1107	20-21	0.21877410480515636
1107	22-23	0.25107288092319635
1107	24-25	0.31159055416503634
1107	26-27	0.28962116250696823
1107	28-29	0.24919002773229693
1107	30-31	0.15113091250162114
1107	32-33	0.19456803571596026
1107	34-35	0.16162598483173696
1107	36-37	0.29807914203231434
1107	38-39	0.2630637845921626
1107	40-41	0.2394223613926414
1107	42-43	0.21246334008823453
1107	44-45	0.25478671689311483
1107	46-47	0.20104494676660778
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2209	5	1.5218270670597462
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2209	8	0.4878796632274742
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2214	96-97	-0.562291726604979
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2214	100	-0.6021720713686101
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2215	3	-3.532376050028315
2215	4	-1.8435708385989003
2215	5	-2.581487034150122
2215	6	-5.1179832824703375
2215	7	-2.186862378210364
2215	8	-1.1712180446199127
2215	9	-1.5491952081613576
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2215	26-27	-0.8585973778054949
2215	28-29	-0.9637907602969875
2215	30-31	-1.007070438032848
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2215	100	-0.9116130018496982
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2216	2	-4.921652631632366
2216	3	-5.902663234442414
2216	4	-3.0783439187285637
2216	5	-4.309568821196571
2216	6	-1.9930377160684358
2216	7	-1.29725732853597
2216	8	-0.8826911812955203
2216	9	-0.6452502545791674
2216	10-11	-1.0102291996901869
2216	12-13	-0.7388694101051954
2216	14-15	-1.6194669365502818
2216	16-17	-1.128365659968658
2216	18-19	-1.6895579276748904
2216	20-21	-1.122016903731641
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2216	26-27	-1.3099915210525879
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2216	30-31	-2.1475142457653504
2216	32-33	-1.3464865022861616
2216	34-35	-1.3887236067569546
2216	36-37	-1.3542283690106132
2216	38-39	-1.2338479344772395
2216	40-41	-1.2457797941405389
2216	42-43	-1.618212743072391
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2216	54-55	-1.0862194835695718
2216	56-57	-0.9857852969782641
2216	58-59	-0.9867792789994141
2216	60-61	-0.9673555468037591
2216	62-63	-0.956001159680234
2216	64-65	-1.0105383808425685
2216	66-67	-1.0393584439756935
2216	68-69	-1.0400624539229035
2216	70-71	-1.0052992533066067
2216	72-73	-1.0431703205829734
2216	74-75	-0.9927564058035507
2216	76-77	-0.8743863475574116
2216	78-79	-0.8686892689307371
2216	80-81	-0.8475745681689126
2216	82-83	-0.9099436476752487
2216	84-85	-0.9026280057664131
2216	86-87	-0.9754221405556258
2216	88-89	-1.0903778108721802
2216	90-91	-1.005889597907963
2216	92-93	-1.0372825678820625
2216	94-95	-1.1108720748197989
2216	96-97	-1.0911031894387158
2216	98-99	-1.1712832802746291
2216	100	-1.255188714627014
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	7922.0
3	6377.0
4	6912.0
5	9259.0
6	7853.0
7	5702.0
8	5360.0
9	5449.0
10	5987.0
11	11598.0
12	7947.0
13	10365.0
14	9689.0
15	10135.0
16	12418.0
17	15591.0
18	21443.0
19	35333.0
20	73063.0
21	100203.0
22	46502.0
23	29148.0
24	27799.0
25	30096.0
26	31212.0
27	34080.0
28	39580.0
29	46065.0
30	53613.0
31	66103.0
32	84658.0
33	115680.0
34	165552.0
35	377534.0
36	456844.0
37	772262.0
38	1064682.0
39	408388.0
40	2927.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	14.48521547686321	44.148128728604966	25.802420440624868	15.564235353906952
2	17.05548318886994	42.51431497326647	23.85655039664714	16.573651441216455
3	18.15825387774614	26.026340981079187	20.98032587352188	34.83507926765279
4	33.33240155770775	27.120403493590057	22.085640761172247	17.461554187529952
5	16.785085083354044	41.34913845893629	23.38831046416403	18.47746599354564
6	35.703691560789714	24.876490377087226	23.32922957237895	16.09058848974411
7	17.860030402733166	24.759560432479706	39.88052583415042	17.499883330636713
8	34.94137275660212	25.2366611383945	22.75391339840444	17.068052706598937
9	34.674087390920064	26.603244332178644	22.61471559562612	16.10795268127517
10-11	16.27445229952354	44.29644346771196	22.831258671731735	16.597845561032763
12-13	16.15411110855794	26.197223103329257	31.705852964384928	25.942812823727873
14-15	16.462141916850396	34.99115563314035	22.804276660607755	25.742425789401498
16-17	25.75253030742717	25.95064345910348	31.54119855732501	16.75562767614434
18-19	16.78687361877095	26.16054036037449	31.60612138683273	25.446464634021826
20-21	25.87266277112234	35.187347270763865	22.623843711390172	16.316146246723626
22-23	25.52345229004492	26.312955492297384	31.60564140339332	16.557950814264384
24-25	16.630524827358954	35.114375063220585	22.59253064969319	25.662569459727276
26-27	16.9456587079026	29.92807204048576	36.582684580055016	16.543584671556623
28-29	24.81849789910741	26.136373596670786	32.509799703930135	16.535328800291662
30-31	22.387324052105335	27.619877921804093	32.94759734806264	17.04520067802794
32-33	18.858932773154034	28.69716264131567	33.96017902205914	18.48372556347116
34-35	19.009682129417	28.534487751853398	34.13176560720861	18.324064511520987
36-37	18.966911541295808	28.45652698463766	34.528436188219224	18.04812528584731
38-39	19.624530704842147	28.24952802467859	34.06014630553817	18.06579496494109
40-41	18.47757399045083	29.557565751094444	33.951608824355425	18.013251434099303
42-43	18.62337819253089	28.40703058646264	34.451141143595635	18.51845007741084
44-45	18.490777098820175	29.106923918542304	33.74201082449701	18.660288158140517
46-47	19.087479449069225	28.500101866285103	34.14132644117104	18.271092243474634
48-49	18.517760439079613	28.78849311219682	34.044163102212686	18.649583346510887
50-51	19.188525923296172	29.358231676077523	33.27736216967685	18.17588023094946
52-53	19.26463810472874	28.994429428310735	33.2470821985357	18.493850268424826
54-55	19.315003249610218	30.040067273505365	31.505240536894597	19.13968893998982
56-57	19.62868494826468	30.791849608200707	30.46052940164793	19.118936041886684
58-59	20.894094303196763	30.867613052270652	29.23742359708416	19.000869047448425
60-61	20.270586093373	31.419990644528937	29.08302998334897	19.22639327874909
62-63	19.6075405134639	31.524185602798795	29.030138222057865	19.83813566167944
64-65	20.023236221972255	31.442448654298655	29.322960741967062	19.211354381762032
66-67	19.956598964146465	30.800484829301656	30.00818315289252	19.234733053659355
68-69	20.38636386251562	30.851039633587263	29.63702025249158	19.125576251405533
70-71	19.147048657054675	31.82454300720532	29.71747317935544	19.310935156384563
72-73	19.170465907398935	30.847355156328454	30.13777002998132	19.844408906291292
74-75	19.360170414017272	31.268814040583727	29.49046597345951	19.88054957193949
76-77	19.9161480953275	30.792992165017324	29.82354853821812	19.467311201437056
78-79	19.6722247547526	31.162009706593985	29.17148031718442	19.994285221468992
80-81	20.13189497869429	31.772268886459727	28.629184914997484	19.4666512198485
82-83	20.15404530314987	31.468046550712376	28.894990347145043	19.48291779899271
84-85	19.8393318364961	32.00886730958412	28.257365191367935	19.894435662551846
86-87	19.836529858750815	32.13823782452351	28.544754218505002	19.480478098220676
88-89	20.997114425963133	31.548855939365634	28.0638391649899	19.390190469681336
90-91	20.38333399272365	32.228396537276915	27.919276884942235	19.468992585057205
92-93	19.900679208556248	32.25310146478148	27.79422054963333	20.051998777028945
94-95	20.2671026811193	32.354622561070805	27.76264694224846	19.61562781556143
96-97	20.167360348935905	31.80215040516296	28.160522835246365	19.869966410654772
98-99	20.783423407800974	31.978870137243188	27.359505092059077	19.878201362896757
100	20.00520231583601	32.847152122964175	27.38983752712797	19.757808034071854
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	166827.0
1	89954.0
2	9409.5
3	4816.5
4	3633.0
5	3187.5
6	3133.0
7	3721.5
8	3559.0
9	2951.5
10	3047.5
11	4756.5
12	42470.0
13	47148.5
14	13853.0
15	12480.0
16	13827.0
17	15411.5
18	16901.5
19	18759.0
20	21176.5
21	23615.5
22	26486.5
23	30706.5
24	34942.0
25	39353.5
26	45795.5
27	52554.5
28	58064.0
29	63330.5
30	69123.0
31	74877.0
32	80513.5
33	87044.5
34	94553.5
35	100959.0
36	106033.0
37	111344.0
38	115630.0
39	119000.5
40	122591.0
41	125775.0
42	127442.5
43	128000.0
44	128637.0
45	128159.0
46	128023.5
47	131113.0
48	132528.0
49	137470.5
50	167567.5
51	162746.0
52	118952.0
53	100273.5
54	92868.0
55	87218.5
56	79755.5
57	69998.5
58	62885.0
59	52970.5
60	41007.0
61	32587.5
62	25415.5
63	19741.5
64	15426.5
65	11691.0
66	9094.0
67	7165.5
68	5619.0
69	4370.5
70	3192.0
71	2443.5
72	2021.5
73	1531.0
74	1096.0
75	825.0
76	605.5
77	381.5
78	232.0
79	146.5
80	86.0
81	46.0
82	28.0
83	18.5
84	14.5
85	11.0
86	7.5
87	5.0
88	2.0
89	1.5
90	1.5
91	1.5
92	1.5
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06147350207789913
2	0.06741949399371903
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	1.42135264919998E-4
18-19	0.0
20-21	2.961151352499958E-4
22-23	1.3029065950999816E-4
24-25	0.0
26-27	0.0022149412116699682
28-29	7.106763245999899E-4
30-31	0.006301330078119911
32-33	0.0015042648870699786
34-35	0.002380765687409966
36-37	0.007758216543549889
38-39	0.01199858528032983
40-41	0.003020374379549957
42-43	0.008196466943719883
44-45	0.0017174677844499758
46-47	0.0026295024010199626
48-49	0.0032572664877499537
50-51	0.009653353409149863
52-53	0.0030914420120099558
54-55	0.0013147512005099813
56-57	8.765008003399875E-4
58-59	0.0010067914598499857
60-61	0.006431620737629908
62-63	0.0018951368655999732
64-65	0.007935885624699887
66-67	0.010991793820479843
68-69	0.012247321993939827
70-71	0.00999684696603986
72-73	0.0047970651910499325
74-75	0.0019425152872399724
76-77	0.004702308347769933
78-79	0.016464001519899765
80-81	0.009404616695539866
82-83	0.0071067632459999
84-85	0.004939200455969931
86-87	0.007473946013709893
88-89	0.003802118336609946
90-91	0.006159194813199913
92-93	0.010956260004249844
94-95	0.008765008003399875
96-97	0.011809071593769833
98-99	0.002404454898229966
100	0.0031743542498799547
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4221331.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.623356722996725
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.59207813822532	50.07009015467253
2	12.966094062163927	15.72096291268987
3	2.7214473305814875	4.949498169740661
4	0.6967198124689892	1.689499749091477
5	0.2696583887410859	0.8173798346999689
6	0.1475523363267127	0.5367070752267535
7	0.09404158059948625	0.39907814012339726
8	0.06529472533324666	0.31667083408060037
9	0.04669682086889748	0.2547826226428545
>10	0.2953852619904492	4.1009479315116755
>50	0.08838622775992164	3.4244607263524545
>100	0.012238676521019495	1.4011658199922667
>500	0.0017311793999253658	0.7711671368414111
>1k	0.002360699134924977	2.4009198773367966
>5k	1.5737994232833147E-4	0.5906055860710161
>10k+	1.5737994232833147E-4	12.556063428926281
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	312594	7.405105167066975	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	169661	4.0191351969319635	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	31703	0.7510190506264492	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	12452	0.2949780531306358	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7389	0.17503957874897752	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6051	0.14334341467181796	No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5747	0.13614189458253806	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA	5574	0.1320436611106781	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.006467154553859909	0.0	0.0	0.0	0.0
2	0.006467154553859909	0.0	0.0	0.0	0.0
3	0.006467154553859909	0.0	0.0	0.0	0.0
4	0.006467154553859909	0.0	0.0	0.0	0.0
5	0.006467154553859909	0.0	0.0	0.0	0.0
6	0.006467154553859909	0.0	0.0	0.0	0.0
7	0.006467154553859909	0.0	0.0	0.0	0.0
8	0.006467154553859909	0.0	0.0	0.0	0.0
9	0.0064908437646799075	0.0	0.0	0.0	0.0
10-11	0.0064908437646799075	0.0	0.0	0.0	0.0
12-13	0.006526377580909907	0.0	0.0	0.0	0.0
14-15	0.006561911397139907	0.0	0.0	0.0	0.0
16-17	0.006597445213369906	0.0	0.0	0.0	0.0
18-19	0.006692202056649905	0.0	0.0	0.0	0.0
20-21	0.006751425083699904	0.0	0.0	0.0	0.0
22-23	0.006846181926979902	0.0	0.0	0.0	0.0
24-25	0.006952783375669901	0.0	0.0	0.0	0.0
26-27	0.007023851008129901	0.0	0.0	0.0	0.0
28-29	0.0070593848243599	0.0	0.0	0.0	0.0
30-31	0.007154141667639898	1.1844605409999832E-5	0.0	0.0	0.0
32-33	0.007225209300099898	2.3689210819999665E-5	0.0	0.0	0.0
34-35	0.007367344565019895	2.3689210819999665E-5	0.0	0.0	0.0
36-37	0.007651615094859891	2.3689210819999665E-5	0.0	0.0	0.0
38-39	0.008113554705849884	2.3689210819999665E-5	0.0	0.0	0.0
40-41	0.008622872738479878	2.3689210819999665E-5	0.0	0.0	0.0
42-43	0.00915587998192987	2.3689210819999665E-5	0.0	0.0	0.0
44-45	0.010056069993089858	2.3689210819999665E-5	0.0	0.0	0.0
46-47	0.010825969344739846	2.3689210819999665E-5	0.0	0.0	0.0
48-49	0.011631402512619834	2.3689210819999665E-5	0.0	0.0	0.0
50-51	0.01278032923738982	2.3689210819999665E-5	0.0	1.1844605409999832E-5	0.0
52-53	0.014379350967739797	2.3689210819999665E-5	0.0	2.3689210819999665E-5	0.0
54-55	0.01667720441727976	2.3689210819999665E-5	0.0	2.3689210819999665E-5	0.0
56-57	0.019093503920919727	2.3689210819999665E-5	0.0	2.3689210819999665E-5	0.0
58-59	0.022113878300469687	2.3689210819999665E-5	0.0	2.3689210819999665E-5	0.0
60-61	0.025939685847899633	2.3689210819999665E-5	0.0	2.3689210819999665E-5	0.0
62-63	0.03102102156878956	2.3689210819999665E-5	0.0	2.3689210819999665E-5	0.0
64-65	0.03790273731199946	2.3689210819999665E-5	0.0	3.55338162299995E-5	0.0
66-67	0.04640716399637934	2.3689210819999665E-5	0.0	4.737842163999933E-5	0.0
68-69	0.05847681690916917	2.3689210819999665E-5	0.0	4.737842163999933E-5	0.0
70-71	0.07443150039643895	2.3689210819999665E-5	0.0	4.737842163999933E-5	0.0
72-73	0.09440150511769865	2.3689210819999665E-5	0.0	4.737842163999933E-5	0.0
74-75	0.12081497518199828	2.3689210819999665E-5	0.0	4.737842163999933E-5	2.3689210819999665E-5
76-77	0.1539206473029478	2.3689210819999665E-5	0.0	4.737842163999933E-5	2.3689210819999665E-5
78-79	0.19528200939466722	2.3689210819999665E-5	1.1844605409999832E-5	7.1067632459999E-5	2.3689210819999665E-5
80-81	0.25028835691870643	2.3689210819999665E-5	2.3689210819999665E-5	7.1067632459999E-5	2.3689210819999665E-5
82-83	0.31946085251310546	2.3689210819999665E-5	2.3689210819999665E-5	7.1067632459999E-5	2.3689210819999665E-5
84-85	0.40699248649300424	2.3689210819999665E-5	2.3689210819999665E-5	7.1067632459999E-5	2.3689210819999665E-5
86-87	0.5141980100589127	2.3689210819999665E-5	2.3689210819999665E-5	7.1067632459999E-5	2.3689210819999665E-5
88	0.6084810691225113	2.3689210819999665E-5	2.3689210819999665E-5	7.1067632459999E-5	2.3689210819999665E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	3165	0.0	86.51371	1
AACGAGT	91710	0.0	69.450195	1
GTGGAAT	101060	0.0	69.39945	6
ACGAGTG	96860	0.0	68.90625	2
GAATCAC	102590	0.0	68.877464	9
TGGAATC	102930	0.0	68.54494	7
AGTGGAA	100335	0.0	68.378784	5
GGAATCA	103410	0.0	68.29494	8
CGAGTGG	99335	0.0	68.21564	3
GAGTGGA	102405	0.0	67.06083	4
ATCGAGT	1240	0.0	65.63872	1
CGTGTGG	2315	0.0	60.49037	3
AACGTGT	2010	0.0	60.155144	1
ACGATTG	575	0.0	58.053387	2
TCGAGTG	1730	0.0	55.983257	2
ACGTGTG	2380	0.0	54.916756	2
CGATTGG	750	0.0	50.12446	3
GACGAGT	895	0.0	49.938587	1
GAGTACT	46945	0.0	41.712177	20-21
AGTACTT	47820	0.0	41.705612	20-21
>>END_MODULE