FastQCFastQC Report
Thu 26 May 2016
SRR2132676_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132676_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4557840
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3082326.762677057553577No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA969322.1267091429273512No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG231420.5077405086619978No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT121120.2657399118880873No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA75600.16586804275709546No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC68040.14928123848138591No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT63540.13940814069822546No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT56400.12374282554894424No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT30300.084.7755051
AACGAGT861500.073.143441
ACGAGTG909450.072.408352
GTGGAAT963350.071.402386
CGAGTGG935250.071.2867053
TGGAATC978100.070.7820367
GAATCAC982800.070.5295949
GGAATCA984750.070.370848
AGTGGAA964500.069.6704945
GAGTGGA968750.069.44734
AACGTGT19350.061.7551461
CGTGTGG25600.058.1881263
ATCGAGT12000.057.2391
ACGTGTG25150.053.4664572
TCGAGTG15100.051.6873782
CGATTGG8300.046.4248853
ACGATTG8000.044.665992
AGTACTT467850.041.13214520-21
GAGTACT460400.041.016920-21
GTACTTT463850.040.0288522-23
Produced by FastQC (version 0.11.5)