##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2132676_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4557840 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.214993944500026 31.0 28.0 34.0 16.0 34.0 2 30.336755129622805 33.0 30.0 34.0 23.0 34.0 3 30.791878828567917 33.0 31.0 34.0 25.0 34.0 4 35.25075847331192 37.0 35.0 37.0 32.0 37.0 5 34.70829274393134 37.0 35.0 37.0 32.0 37.0 6 35.26116164674495 37.0 35.0 37.0 32.0 37.0 7 35.672723483053375 37.0 35.0 37.0 35.0 37.0 8 35.87461516858863 37.0 35.0 37.0 35.0 37.0 9 37.79526266828147 39.0 39.0 39.0 35.0 39.0 10-11 37.8848825320766 39.0 39.0 39.0 35.0 39.0 12-13 37.888343601355025 39.0 39.0 39.0 35.0 39.0 14-15 39.321334667298544 41.0 39.0 41.0 37.0 41.0 16-17 39.27216488512102 41.0 39.0 41.0 36.5 41.0 18-19 39.19505127428782 41.0 39.0 41.0 36.0 41.0 20-21 39.05972982816422 41.0 39.0 41.0 35.0 41.0 22-23 38.979913511663426 40.5 39.0 41.0 35.0 41.0 24-25 38.915978072946835 41.0 39.0 41.0 35.0 41.0 26-27 38.86803167728573 41.0 39.0 41.0 35.0 41.0 28-29 38.70809473347024 41.0 39.0 41.0 35.0 41.0 30-31 38.593026521334664 41.0 39.0 41.0 35.0 41.0 32-33 38.462044959893284 40.0 38.0 41.0 35.0 41.0 34-35 38.380532664595506 40.0 38.0 41.0 35.0 41.0 36-37 38.22765926403736 40.0 38.0 41.0 34.5 41.0 38-39 37.80052294507925 40.0 36.5 41.0 34.0 41.0 40-41 37.59759809471153 40.0 35.5 41.0 33.0 41.0 42-43 37.45579232706721 40.0 35.0 41.0 33.0 41.0 44-45 37.311168119109055 40.0 35.0 41.0 33.0 41.0 46-47 37.16111930212557 40.0 35.0 41.0 33.0 41.0 48-49 36.995248078036965 40.0 35.0 41.0 33.0 41.0 50-51 36.72457556649641 40.0 35.0 41.0 32.5 41.0 52-53 36.24108239868008 39.0 35.0 41.0 30.5 41.0 54-55 35.42834939357239 39.0 35.0 41.0 27.0 41.0 56-57 34.7722162252295 39.0 35.0 41.0 22.5 41.0 58-59 34.42802939550313 39.0 35.0 41.0 20.0 41.0 60-61 34.16365778526671 38.0 35.0 40.5 19.0 41.0 62-63 33.85313690256788 38.0 34.5 40.0 17.5 41.0 64-65 33.57999348375546 37.0 34.0 40.0 14.5 41.0 66-67 33.26468217401225 36.5 34.0 40.0 12.0 41.0 68-69 32.96153890878135 36.0 34.0 39.5 9.0 41.0 70-71 32.61630037473891 35.5 34.0 39.0 7.0 41.0 72-73 32.26623630930441 35.0 33.0 39.0 3.5 40.5 74-75 31.914453995752375 35.0 33.0 37.5 2.0 40.0 76-77 31.54664259824829 35.0 33.0 37.0 2.0 39.5 78-79 31.430274099134678 35.0 33.0 37.0 2.0 39.0 80-81 31.289822920506204 35.0 33.0 36.0 2.0 39.0 82-83 30.97883866041809 35.0 33.0 36.0 2.0 38.0 84-85 30.694092486791988 35.0 33.0 36.0 2.0 37.0 86-87 30.493607278886493 35.0 33.0 35.0 2.0 37.0 88-89 30.2791323302266 35.0 33.0 35.0 2.0 36.5 90-91 30.079935561581802 35.0 33.0 35.0 2.0 36.0 92-93 29.912234742772895 35.0 33.0 35.0 2.0 36.0 94-95 29.732026793393363 35.0 33.0 35.0 2.0 36.0 96-97 29.554398026258053 35.0 33.0 35.0 2.0 35.5 98-99 29.400873549751637 35.0 33.0 35.0 2.0 35.0 100 29.28475220718586 35.0 32.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 2.772439798175146 1101 2 2.245823257767732 1101 3 2.0414919919350716 1101 4 1.0134286595371762 1101 5 1.615122828007209 1101 6 1.0671050690963568 1101 7 0.5446565385023163 1101 8 0.41162706118237224 1101 9 0.3769558479369124 1101 10-11 0.3626990063184792 1101 12-13 0.3029439084694232 1101 14-15 0.4328101272296365 1101 16-17 0.4056079067263312 1101 18-19 0.408019452137367 1101 20-21 0.4810226949678622 1101 22-23 0.411805618159903 1101 24-25 0.48297208543941395 1101 26-27 0.44390448323981957 1101 28-29 0.4437700799587745 1101 30-31 0.47074193802767894 1101 32-33 0.4204654494128661 1101 34-35 0.4514454374708947 1101 36-37 0.4457439546593278 1101 38-39 0.4630450955763976 1101 40-41 0.5014507198055398 1101 42-43 0.47683854685013216 1101 44-45 0.4463357462675148 1101 46-47 0.43096769889276487 1101 48-49 0.33048215340449616 1101 50-51 0.36552876198192763 1101 52-53 0.34858856624585854 1101 54-55 0.43057814586065035 1101 56-57 0.46883839152640405 1101 58-59 0.45369270070420953 1101 60-61 0.47124510120399066 1101 62-63 0.46356591385105617 1101 64-65 0.4634266796834723 1101 66-67 0.4620423674666725 1101 68-69 0.4384802120178506 1101 70-71 0.4445232051445771 1101 72-73 0.459301682308876 1101 74-75 0.5236009929551955 1101 76-77 0.41593629364762563 1101 78-79 0.5013572325408404 1101 80-81 0.5295302766112009 1101 82-83 0.5691308210714929 1101 84-85 0.6105173302376663 1101 86-87 0.5449657523442468 1101 88-89 0.5972484737475732 1101 90-91 0.4924051312541131 1101 92-93 0.5927290148727664 1101 94-95 0.5798106755109202 1101 96-97 0.6118674112246758 1101 98-99 0.5892877693842387 1101 100 0.6122394469127315 1102 1 2.8372488052656486 1102 2 2.298353312960309 1102 3 2.0526914598734685 1102 4 1.0178092135572427 1102 5 1.5526970391036201 1102 6 0.9775953159637183 1102 7 0.47373370786932867 1102 8 0.41022148470877795 1102 9 0.3984340597475651 1102 10-11 0.3201763385804597 1102 12-13 0.3013188175568544 1102 14-15 0.39006209104194056 1102 16-17 0.3725346646769765 1102 18-19 0.386473568401442 1102 20-21 0.3978704413140619 1102 22-23 0.3962576178592627 1102 24-25 0.4517330671907729 1102 26-27 0.3803913721414034 1102 28-29 0.5265410226491909 1102 30-31 0.48963467229268787 1102 32-33 0.46694741261389083 1102 34-35 0.4947120359591608 1102 36-37 0.5575155677326364 1102 38-39 0.4256247791861938 1102 40-41 0.40686402655509113 1102 42-43 0.4918351409998536 1102 44-45 0.5250332293235473 1102 46-47 0.5505588561004444 1102 48-49 0.5251670252113101 1102 50-51 0.4419730867443761 1102 52-53 0.40253552224360334 1102 54-55 0.44946428066040767 1102 56-57 0.4030794780167568 1102 58-59 0.3469731756096266 1102 60-61 0.4151069254154578 1102 62-63 0.4417797196868065 1102 64-65 0.48269707437709997 1102 66-67 0.43610901233099497 1102 68-69 0.44609422994857084 1102 70-71 0.36669160043263815 1102 72-73 0.3635106962750996 1102 74-75 0.30605307404066195 1102 76-77 0.3221065546768678 1102 78-79 0.4037735708347796 1102 80-81 0.490718174656525 1102 82-83 0.5734446349844653 1102 84-85 0.45145376278512117 1102 86-87 0.4219215510115326 1102 88-89 0.4393199814311508 1102 90-91 0.4049318393557755 1102 92-93 0.48339607961215947 1102 94-95 0.48401854937019095 1102 96-97 0.4668150237486266 1102 98-99 0.4727845260869046 1102 100 0.4256485342141403 1103 1 2.51415199704131 1103 2 2.1172338339786663 1103 3 1.9627221612029224 1103 4 0.9567241605403964 1103 5 1.5551423160291833 1103 6 1.0044357271806845 1103 7 0.4977277872062089 1103 8 0.43153940278833147 1103 9 0.35351564648527756 1103 10-11 0.28156126780162793 1103 12-13 0.2581028855906524 1103 14-15 0.3931909031416225 1103 16-17 0.4160250678203141 1103 18-19 0.3861304390618656 1103 20-21 0.45558846704203404 1103 22-23 0.41440215739314823 1103 24-25 0.4972469915139328 1103 26-27 0.44957058523144156 1103 28-29 0.46335875234400703 1103 30-31 0.4772226587289907 1103 32-33 0.45860321755495903 1103 34-35 0.41123695118602654 1103 36-37 0.3849293574952739 1103 38-39 0.45358631640564084 1103 40-41 0.4322017088845982 1103 42-43 0.4912911074135522 1103 44-45 0.38746576067615734 1103 46-47 0.4492302270030777 1103 48-49 0.4021354842241678 1103 50-51 0.35513674463769007 1103 52-53 0.3685518740386371 1103 54-55 0.37330530547336593 1103 56-57 0.37663082093079936 1103 58-59 0.3763067565343263 1103 60-61 0.33945106665018443 1103 62-63 0.3297694500275128 1103 64-65 0.3419476260752532 1103 66-67 0.2715838172182288 1103 68-69 0.2647230581955142 1103 70-71 0.2743134638267932 1103 72-73 0.347684989832338 1103 74-75 0.35401659199102653 1103 76-77 0.3493384509037938 1103 78-79 0.3466771716744361 1103 80-81 0.3395635699953452 1103 82-83 0.4002107257320837 1103 84-85 0.36687953381693106 1103 86-87 0.4361500024890077 1103 88-89 0.4423060194310402 1103 90-91 0.4067113000371876 1103 92-93 0.3988668907430082 1103 94-95 0.4766171068813563 1103 96-97 0.45969963540695247 1103 98-99 0.48115997148224565 1103 100 0.5112527540322134 1104 1 1.556180112824464 1104 2 1.7527714739442075 1104 3 1.8237967625131866 1104 4 0.8502914196952531 1104 5 1.5110184520665584 1104 6 0.9771970353528445 1104 7 0.4624501529828393 1104 8 0.3871752652893079 1104 9 0.3157431515096789 1104 10-11 0.22457452178851867 1104 12-13 0.21636597122738266 1104 14-15 0.3442878049765099 1104 16-17 0.2942755206692169 1104 18-19 0.28237322871550674 1104 20-21 0.382846567425716 1104 22-23 0.29174841584327993 1104 24-25 0.3597041105074936 1104 26-27 0.26682052128448674 1104 28-29 0.3558603784239267 1104 30-31 0.398891585725039 1104 32-33 0.3711375949203415 1104 34-35 0.3681541646644817 1104 36-37 0.31755672679550884 1104 38-39 0.3569725612833565 1104 40-41 0.27790907714079793 1104 42-43 0.2691340641123219 1104 44-45 0.3104971599401054 1104 46-47 0.3684049509871059 1104 48-49 0.34218366630336305 1104 50-51 0.34509923256607067 1104 52-53 0.343136699032776 1104 54-55 0.31847266903143634 1104 56-57 0.35815931517556976 1104 58-59 0.3624099277816839 1104 60-61 0.2981056851869752 1104 62-63 0.2935165750242561 1104 64-65 0.29255441750375155 1104 66-67 0.3165719204729953 1104 68-69 0.2936959916645776 1104 70-71 0.28457433611556837 1104 72-73 0.2217840580337409 1104 74-75 0.27067092863916287 1104 76-77 0.29035636127363773 1104 78-79 0.2526012183426687 1104 80-81 0.30397384327073596 1104 82-83 0.37543249380036414 1104 84-85 0.36550744601383656 1104 86-87 0.3013790683413262 1104 88-89 0.3477522857114188 1104 90-91 0.30582874842285435 1104 92-93 0.29275882298532707 1104 94-95 0.23091906183137212 1104 96-97 0.260032932199163 1104 98-99 0.31931587678853646 1104 100 0.3360188229905319 1105 1 2.273302382249323 1105 2 1.9932191447131302 1105 3 1.9353712623842334 1105 4 0.9783372062517444 1105 5 1.5439279349300605 1105 6 1.0196904780795037 1105 7 0.5282758098050806 1105 8 0.363683316501735 1105 9 0.3124399402705862 1105 10-11 0.23982742085760833 1105 12-13 0.20443745158962656 1105 14-15 0.29546306544259693 1105 16-17 0.2152777129153236 1105 18-19 0.22947276058214783 1105 20-21 0.2875619647307843 1105 22-23 0.2839267079838024 1105 24-25 0.3872174075385857 1105 26-27 0.3088164506654465 1105 28-29 0.2634582387333282 1105 30-31 0.30277114357747337 1105 32-33 0.29193649952721756 1105 34-35 0.2522563656698509 1105 36-37 0.2865899121948061 1105 38-39 0.20028986031935148 1105 40-41 0.12938537606487444 1105 42-43 0.21403599698724918 1105 44-45 0.2568098798338312 1105 46-47 0.2817907303415481 1105 48-49 0.2499097523289464 1105 50-51 0.3118320194194055 1105 52-53 0.2680573183118824 1105 54-55 0.14316755508816215 1105 56-57 0.11377016350912328 1105 58-59 0.10045683357592594 1105 60-61 0.22009621302922966 1105 62-63 0.210241150078879 1105 64-65 0.18286930044607885 1105 66-67 0.1831104222516089 1105 68-69 0.14059491233008714 1105 70-71 0.12852953984116766 1105 72-73 0.1796993195703962 1105 74-75 0.18973148670084328 1105 76-77 0.1755658777142699 1105 78-79 0.12385590819215508 1105 80-81 0.10010681601486482 1105 82-83 0.18136013507979598 1105 84-85 0.13650860418159638 1105 86-87 0.09901096807708853 1105 88-89 0.1853431021223635 1105 90-91 0.22010328771053622 1105 92-93 0.1823352523249291 1105 94-95 0.1289644104252723 1105 96-97 0.1105136569786005 1105 98-99 0.09482294015198889 1105 100 0.0985078130974415 1106 1 1.709184952533473 1106 2 1.1258223371722202 1106 3 1.5212000183638175 1106 4 0.778670407794948 1106 5 1.5097734235498237 1106 6 1.0014488800927523 1106 7 0.5299177344430888 1106 8 0.39886033889194294 1106 9 0.31689381921507476 1106 10-11 0.23464575443413338 1106 12-13 0.20688502186592928 1106 14-15 0.28218848462151414 1106 16-17 0.2569627164532662 1106 18-19 0.24627661057987638 1106 20-21 0.32676670541016506 1106 22-23 0.2618869065789724 1106 24-25 0.31593753706945904 1106 26-27 0.2618951819343849 1106 28-29 0.3019183847693441 1106 30-31 0.2471882036533799 1106 32-33 0.2322537489729939 1106 34-35 0.2512211071023174 1106 36-37 0.2349604801632168 1106 38-39 0.20848920701683227 1106 40-41 0.2420646459475364 1106 42-43 0.297179077926792 1106 44-45 0.23638762581462203 1106 46-47 0.2312452329357697 1106 48-49 0.16418774243527423 1106 50-51 0.11908316356173287 1106 52-53 0.17117954816956882 1106 54-55 0.16261707240679613 1106 56-57 0.1359026494632758 1106 58-59 0.08287355868524315 1106 60-61 0.15641445560779488 1106 62-63 0.1926390872495034 1106 64-65 0.16796832739559164 1106 66-67 0.14764936466716705 1106 68-69 0.15712996536776558 1106 70-71 0.1792781352564461 1106 72-73 0.1913227975120222 1106 74-75 0.19941502172014935 1106 76-77 0.11258760896594566 1106 78-79 0.09741045283371363 1106 80-81 0.08852036482506875 1106 82-83 0.08529303115229325 1106 84-85 0.041184055288240984 1106 86-87 0.050262405609352356 1106 88-89 0.04007915315652255 1106 90-91 0.0534500746200095 1106 92-93 0.06699607708013744 1106 94-95 0.06241568808354714 1106 96-97 0.06835887652684036 1106 98-99 0.06226038298409975 1106 100 0.05424719497033692 1107 1 1.5004346949885239 1107 2 0.9919254913757705 1107 3 1.3831952727409949 1107 4 0.6775111157431439 1107 5 1.390118774720662 1107 6 0.9388514416108578 1107 7 0.4622261186856349 1107 8 0.38757898211890307 1107 9 0.35543466761986764 1107 10-11 0.15112041432806933 1107 12-13 0.09064442669693307 1107 14-15 0.19919421995828657 1107 16-17 0.15732411726919793 1107 18-19 0.1674404805220675 1107 20-21 0.16290556684990065 1107 22-23 0.15893715587630908 1107 24-25 0.20121719072518118 1107 26-27 0.16830078039779295 1107 28-29 0.19467151399837235 1107 30-31 0.151454866232271 1107 32-33 0.12165219116199211 1107 34-35 0.08379611793696995 1107 36-37 0.16615265610989383 1107 38-39 0.12458733774247577 1107 40-41 0.1342311557447502 1107 42-43 0.1240797210246285 1107 44-45 0.13155449563418387 1107 46-47 0.1553396516162806 1107 48-49 0.14447904777963316 1107 50-51 0.06654142660755724 1107 52-53 0.15043353042452878 1107 54-55 0.1523460679367119 1107 56-57 0.12133160968598133 1107 58-59 0.11969030391019686 1107 60-61 0.13171906139181289 1107 62-63 0.16853811217738723 1107 64-65 0.16069133120216605 1107 66-67 0.07482783582475605 1107 68-69 0.041210316765642574 1107 70-71 0.02097838444565525 1107 72-73 0.07100133122176544 1107 74-75 0.05588966308012289 1107 76-77 0.12291769046289858 1107 78-79 0.10394701695707553 1107 80-81 0.13739675838649035 1107 82-83 0.1482620757169144 1107 84-85 0.16271820304536888 1107 86-87 0.11396182370449992 1107 88-89 0.04749824311392459 1107 90-91 0.03593750086639602 1107 92-93 0.06331828117682292 1107 94-95 0.00550095762460856 1107 96-97 -0.0016476899040007709 1107 98-99 0.04625948609443853 1107 100 0.08840132314941584 1108 1 0.5695730336495011 1108 2 0.2213319948351007 1108 3 -0.05403288084533742 1108 4 0.05195013314040153 1108 5 1.0910899715578424 1108 6 0.7381338281464167 1108 7 0.30741430930338254 1108 8 0.25185764200176664 1108 9 0.22658904752837117 1108 10-11 0.09083566203215554 1108 12-13 0.0647795906039974 1108 14-15 0.08958841767759651 1108 16-17 0.07698011485946665 1108 18-19 0.04933883374157233 1108 20-21 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2215 52-53 -0.9163771692484914 2215 54-55 -0.896010428087898 2215 56-57 -0.8821206535181858 2215 58-59 -0.8178585527031146 2215 60-61 -0.8037460027679728 2215 62-63 -0.7377408497465865 2215 64-65 -0.7386443514569478 2215 66-67 -0.7140298588303651 2215 68-69 -0.7390732663664181 2215 70-71 -0.7138849365639501 2215 72-73 -0.733115987028274 2215 74-75 -0.7630100396714639 2215 76-77 -0.769023256160132 2215 78-79 -0.710167324326541 2215 80-81 -0.7004201652754283 2215 82-83 -0.6680612811059419 2215 84-85 -0.6649997262111533 2215 86-87 -0.6705860864053079 2215 88-89 -0.6988868250649567 2215 90-91 -0.6570475130771669 2215 92-93 -0.6784972462616956 2215 94-95 -0.7197217172226757 2215 96-97 -0.7395337243830262 2215 98-99 -0.7576984692039979 2215 100 -0.873673114457926 2216 1 -5.834462665715289 2216 2 -4.791255315581452 2216 3 -5.788836783078601 2216 4 -2.9724054582279322 2216 5 -4.164661789542663 2216 6 -1.9229015162210743 2216 7 -1.182753151210214 2216 8 -0.7790834711119601 2216 9 -0.6640010690572424 2216 10-11 -1.0088856530194903 2216 12-13 -0.7768965278990905 2216 14-15 -1.7291428211985505 2216 16-17 -1.1503321169304215 2216 18-19 -1.6853504993545627 2216 20-21 -1.1285546414623653 2216 22-23 -0.8894676373282593 2216 24-25 -1.5695647386913407 2216 26-27 -1.3130610863228043 2216 28-29 -1.955135089298217 2216 30-31 -2.0016887782211583 2216 32-33 -1.2033111519465152 2216 34-35 -1.2197912013066414 2216 36-37 -1.2673087656718849 2216 38-39 -1.2097702818348282 2216 40-41 -1.2077548893558685 2216 42-43 -1.5168748749911458 2216 44-45 -1.370337758385432 2216 46-47 -1.7864782773957515 2216 48-49 -1.3797941345441558 2216 50-51 -1.212547712840312 2216 52-53 -1.109604137691555 2216 54-55 -0.9518694745852798 2216 56-57 -0.8818196189518943 2216 58-59 -0.8870785807090726 2216 60-61 -0.8322181415609577 2216 62-63 -0.7787050367760031 2216 64-65 -0.7972429897605267 2216 66-67 -0.8976554789694546 2216 68-69 -0.8868751822736911 2216 70-71 -0.8429487062611827 2216 72-73 -0.8215724881735227 2216 74-75 -0.867542938800753 2216 76-77 -0.7749448335536897 2216 78-79 -0.7138770805249735 2216 80-81 -0.7348628229665302 2216 82-83 -0.7264573094585351 2216 84-85 -0.7501192931625411 2216 86-87 -0.7882388538159795 2216 88-89 -0.8347332767566407 2216 90-91 -0.7727678788484411 2216 92-93 -0.8149858951807474 2216 94-95 -0.8718122186318382 2216 96-97 -0.8321854085813207 2216 98-99 -0.8881057176673188 2216 100 -0.9753841961144118 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6450.0 3 4289.0 4 4867.0 5 6417.0 6 5383.0 7 4206.0 8 4219.0 9 4597.0 10 5295.0 11 10031.0 12 7624.0 13 10000.0 14 10151.0 15 10851.0 16 12829.0 17 16564.0 18 22752.0 19 38374.0 20 79090.0 21 105562.0 22 47796.0 23 29241.0 24 28652.0 25 31219.0 26 32247.0 27 35364.0 28 41339.0 29 48593.0 30 57327.0 31 71156.0 32 92634.0 33 123871.0 34 180157.0 35 349094.0 36 520201.0 37 903692.0 38 1164495.0 39 428449.0 40 2762.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 14.652622427899972 41.269294180407584 26.020816090868976 18.05726730082347 2 17.993025722451154 39.078436361967775 25.42722005683314 17.501317858747935 3 19.098985286667652 25.248083576449275 20.922042222202233 34.73088891468084 4 32.318291120355255 26.129043582047633 22.706545205623716 18.84612009197339 5 18.072573850771416 38.34774805609675 23.845549646323697 19.73412844680814 6 35.31291137907429 23.63922384287294 23.8144164779808 17.233448300071963 7 19.044635178066805 23.68902813613466 38.624765239674936 18.641571446123603 8 34.04704860196935 23.888442771137207 23.77334439120285 18.29116423569059 9 33.77716198901234 25.126704754883892 23.805245467151106 17.29088778895266 10-11 17.36786504133537 41.24637986414618 23.502909272813437 17.88284582170502 12-13 17.344432889263338 24.980067312586666 31.385085917890933 26.290413880259074 14-15 17.666668860688397 32.872819142400786 23.31064714864936 26.149864848261455 16-17 25.968872299637713 24.664701187789166 31.34847391992365 18.017952592649475 18-19 17.858009495726044 24.856664560405804 31.398491390658734 25.886834553209415 20-21 26.097970536194914 33.055361109931816 23.279666341182793 17.56700201269047 22-23 25.743459035965223 25.022996123048376 31.441835249137558 17.791709591848846 24-25 17.750304969020412 32.943433731767676 23.271352219472384 26.034909079739528 26-27 18.05366042562808 28.262097291746883 35.928975686388085 17.755266596236947 28-29 25.203488738828923 24.786808130883106 32.31460521314251 17.695097917145468 30-31 23.188431236661554 25.94288354803026 32.597667364373635 18.271017850934555 32-33 19.92545692600599 27.136980989047814 33.56936263203421 19.36819945291198 34-35 19.865213707646575 26.958114159153883 33.69387437856996 19.482797754629576 36-37 19.768340590106366 27.075755795462236 33.97916364278267 19.176739971648722 38-39 20.24427557632627 26.808072199349127 33.6419098183979 19.305742405926704 40-41 19.468765005511003 27.79611121880431 33.427777889310626 19.307345886374062 42-43 19.645197105405206 27.019732482567044 33.786681616514464 19.54838879551329 44-45 19.642151125925402 27.52891660424568 33.171038768588616 19.657893501240302 46-47 20.025679489517024 27.10962250228897 33.410301591386755 19.454396416807253 48-49 19.63520112548255 27.44721859617686 33.335169058796126 19.582411219544465 50-51 20.03467984230052 27.802734123220407 32.746372522505965 19.416213511973105 52-53 20.196172153796212 27.616558466526996 32.49767069016794 19.68959868950884 54-55 20.32079917968719 28.372658272728994 31.106811253345523 20.199731294238294 56-57 20.63443429007603 28.994285301806777 29.949832249424258 20.421448158692932 58-59 21.384112964508752 29.297572253016295 28.98825537094714 20.330059411527813 60-61 21.178343372552202 29.592044396733545 28.69629849858778 20.53331373212647 62-63 20.629564681276573 29.71448098596841 28.681482814172117 20.9744715185829 64-65 20.91562583036696 29.657487822747385 29.04149953274545 20.385386814140205 66-67 20.84394352747255 29.0929822740547 29.526909500319377 20.53616469815337 68-69 21.094717633914982 29.246204934471137 29.268499000442482 20.390578431171406 70-71 20.112584658192702 29.936396437375457 29.398619225713603 20.552399678718245 72-73 20.201227767597636 29.300809643802143 29.64205123220522 20.855911356394998 74-75 20.486868976750838 29.5489929693472 29.104750232351183 20.859387821550783 76-77 20.894144838702847 29.145640106472037 29.304889593348822 20.65532546147629 78-79 20.855466470751782 29.556760127809884 28.725460442770274 20.86231295866806 80-81 21.056371590656255 29.903385744773008 28.4481377767421 20.592104887828636 82-83 20.992565353893085 30.018243500540372 28.45066723334994 20.538523912216608 84-85 20.95506887000057 30.24094763734094 27.9880555778321 20.815927914826382 86-87 20.87085817024161 30.468801683561413 28.104820611998093 20.555519534198876 88-89 21.69705692949554 30.020547847522465 27.774173341334524 20.508221881647472 90-91 21.229550092434135 30.49612298836035 27.6569891035123 20.617337815693208 92-93 20.841893712835994 30.641989514481388 27.599947162079225 20.916169610603394 94-95 21.055203437818882 30.768759855484166 27.52373626814882 20.65230043854813 96-97 20.971587392649482 30.273172280540418 27.78004336095809 20.97519696585201 98-99 21.44354993091396 30.37450173853279 27.107003552761277 21.07494477779198 100 21.004994408360186 30.949797671938263 27.165298614629275 20.879909305072278 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 90099.0 1 49287.5 2 6393.0 3 3753.5 4 3055.5 5 2735.5 6 2693.0 7 3039.0 8 2896.5 9 2642.0 10 2784.0 11 4131.5 12 34293.5 13 38515.0 14 12781.0 15 12316.0 16 13600.5 17 15238.0 18 17034.5 19 18798.0 20 21068.0 21 23700.0 22 26844.0 23 31344.5 24 36235.0 25 41162.0 26 49025.0 27 57094.0 28 61910.0 29 66000.5 30 71235.5 31 77585.0 32 84887.5 33 93346.0 34 101825.5 35 108984.0 36 114878.0 37 119725.5 38 124342.5 39 127418.0 40 129406.5 41 131221.0 42 131751.5 43 132585.0 44 133584.5 45 134417.0 46 138133.0 47 143466.5 48 144176.0 49 149408.5 50 174875.0 51 169847.5 52 135663.0 53 123919.5 54 119865.5 55 116206.0 56 105742.0 57 91413.5 58 83991.5 59 71795.0 60 56893.5 61 46789.0 62 37492.0 63 29437.5 64 22711.5 65 17255.5 66 13399.0 67 10773.5 68 8458.0 69 6307.0 70 4579.5 71 3365.0 72 2644.0 73 1976.0 74 1386.5 75 1037.5 76 764.0 77 545.5 78 361.0 79 203.0 80 131.5 81 86.0 82 45.5 83 26.0 84 19.0 85 14.0 86 10.0 87 7.0 88 2.5 89 0.5 90 2.0 91 2.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.059457988871921795 2 0.06808049426921524 3 2.19402172959121E-5 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 5.485054323978025E-5 18-19 0.0 20-21 2.3037228160707702E-4 22-23 1.4261141242342864E-4 24-25 0.0 26-27 0.0023037228160707702 28-29 7.130570621171431E-4 30-31 0.006395573341758377 32-33 0.0015906657539536272 34-35 0.0023366331420146386 36-37 0.008194671160023169 38-39 0.012483983641373986 40-41 0.0030606603127797376 42-43 0.008337282572446598 44-45 0.001711336949081144 46-47 0.0025670054236217156 48-49 0.0033020027030347707 50-51 0.009598845066961542 52-53 0.0034994646586979797 54-55 0.0014590244501781545 56-57 6.911168448212312E-4 58-59 0.0011957418426272093 60-61 0.006527214645533849 62-63 0.0019197690133923085 64-65 0.00805205974759974 66-67 0.011167570603619259 68-69 0.01258271461920559 70-71 0.01030093202043073 72-73 0.004793937479156793 74-75 0.0018429782528566162 76-77 0.004640355958085409 78-79 0.016652624927597285 80-81 0.0096098151756095 82-83 0.0071634809471153 84-85 0.0050791603040036505 86-87 0.007865567900584488 88-89 0.004058940199743738 90-91 0.005934828778544223 92-93 0.011123690169027435 94-95 0.009116160286451477 96-97 0.011288241798746775 98-99 0.0025779755322696716 100 0.0031374510733154304 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4557840.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.72581208472819 #Duplication Level Percentage of deduplicated Percentage of total 1 83.32984476848021 51.4360233922878 2 12.085305290151924 14.919505666529783 3 2.59753689537257 4.810052228607466 4 0.7360946402850895 1.817441577712521 5 0.3152500105916845 0.9729531456745443 6 0.18634958600355203 0.6901547716633288 7 0.12177375713390282 0.526160883478903 8 0.08198354354244748 0.4048400642192996 9 0.060837650986959785 0.33797280712476224 >10 0.39463578329925164 5.713161539907343 >50 0.06124553085149583 2.525920744553644 >100 0.025192152122158716 2.7663985264387665 >500 0.0016878945028714469 0.740235110582862 >1k 0.001975195510657362 1.9919822303467143 >5k 1.4365058259325735E-4 0.5842875955918302 >10k+ 1.4365058259325735E-4 9.762909715280472 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 308232 6.762677057553577 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 96932 2.1267091429273512 No Hit AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG 23142 0.5077405086619978 No Hit TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 12112 0.2657399118880873 No Hit AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA 7560 0.16586804275709546 No Hit AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC 6804 0.14928123848138591 No Hit ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 6354 0.13940814069822546 No Hit AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 5640 0.12374282554894424 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0074157934460182895 0.0 0.0 0.0 0.0 2 0.0074157934460182895 0.0 0.0 0.0 0.0 3 0.0074157934460182895 0.0 0.0 0.0 0.0 4 0.0074157934460182895 0.0 0.0 0.0 0.0 5 0.0074157934460182895 0.0 0.0 0.0 0.0 6 0.0074157934460182895 0.0 0.0 0.0 0.0 7 0.0074157934460182895 0.0 0.0 0.0 2.19402172959121E-5 8 0.0074157934460182895 0.0 0.0 0.0 2.19402172959121E-5 9 0.0074157934460182895 0.0 0.0 0.0 2.19402172959121E-5 10-11 0.0074157934460182895 0.0 0.0 0.0 2.19402172959121E-5 12-13 0.0074267635546662455 5.4850543239780245E-5 0.0 0.0 4.38804345918242E-5 14-15 0.0074706439892580695 8.77608691836484E-5 0.0 0.0 4.38804345918242E-5 16-17 0.007503554315201938 1.0970108647956049E-4 0.0 0.0 6.58206518877363E-5 18-19 0.007569374967089674 1.316413037754726E-4 0.0 0.0 6.58206518877363E-5 20-21 0.007657135836273322 1.974619556632089E-4 0.0 0.0 8.77608691836484E-5 22-23 0.007679076053569235 2.1940217295912098E-4 0.0 0.0 8.77608691836484E-5 24-25 0.007733926596809015 2.3037228160707702E-4 0.0 0.0 8.77608691836484E-5 26-27 0.007766836922752883 2.413423902550331E-4 0.0 0.0 1.0970108647956049E-4 28-29 0.007810717357344707 2.852228248468573E-4 0.0 0.0 1.4261141242342864E-4 30-31 0.00783265757464062 3.0716304214276936E-4 0.0 2.19402172959121E-5 1.5358152107138468E-4 32-33 0.007931388552472224 3.0716304214276936E-4 0.0 2.19402172959121E-5 1.755217383672968E-4 34-35 0.008073999964895651 3.0716304214276936E-4 0.0 5.4850543239780245E-5 2.1940217295912098E-4 36-37 0.008293402137854775 3.291032594386815E-4 0.0 7.679076053569234E-5 2.1940217295912098E-4 38-39 0.008644445614589366 3.510434767345936E-4 0.0 8.77608691836484E-5 2.3037228160707702E-4 40-41 0.009302652133466729 3.510434767345936E-4 0.0 8.77608691836484E-5 2.632826075509452E-4 42-43 0.010158320608007303 3.729836940305057E-4 0.0 8.77608691836484E-5 2.852228248468573E-4 44-45 0.011233391255506995 3.949239113264178E-4 0.0 8.77608691836484E-5 2.852228248468573E-4 46-47 0.01227555157706282 4.936548891580222E-4 0.0 8.77608691836484E-5 2.852228248468573E-4 48-49 0.013734576027240975 5.046249978059783E-4 0.0 8.77608691836484E-5 2.852228248468573E-4 50-51 0.015423972759026204 5.155951064539344E-4 0.0 8.77608691836484E-5 2.852228248468573E-4 52-53 0.017486353184841943 5.265652151018904E-4 0.0 8.77608691836484E-5 2.852228248468573E-4 54-55 0.02065671458410124 5.265652151018904E-4 0.0 9.873097783160445E-5 2.852228248468573E-4 56-57 0.024386551524406296 5.265652151018904E-4 0.0 1.0970108647956049E-4 2.852228248468573E-4 58-59 0.02870877433170098 5.485054323978025E-4 0.0 1.316413037754726E-4 2.852228248468573E-4 60-61 0.03429255963351061 6.143260842855387E-4 0.0 1.316413037754726E-4 2.852228248468573E-4 62-63 0.04204842644761554 6.143260842855387E-4 0.0 1.316413037754726E-4 2.961929334948133E-4 64-65 0.05254682042370948 6.252961929334948E-4 0.0 1.316413037754726E-4 3.0716304214276936E-4 66-67 0.06551348884559353 6.691766275253191E-4 0.0 1.6455162971934072E-4 3.0716304214276936E-4 68-69 0.08270364909694065 6.801467361732751E-4 0.0 1.755217383672968E-4 3.0716304214276936E-4 70-71 0.10627841258139821 7.130570621171433E-4 0.0 1.755217383672968E-4 3.0716304214276936E-4 72-73 0.1363803907113896 7.459673880610113E-4 0.0 1.755217383672968E-4 3.0716304214276936E-4 74-75 0.17307540413880257 8.337282572446598E-4 0.0 1.755217383672968E-4 3.0716304214276936E-4 76-77 0.2226712653362119 8.337282572446598E-4 0.0 1.755217383672968E-4 3.0716304214276936E-4 78-79 0.2866708791883875 8.446983658926157E-4 0.0 1.755217383672968E-4 3.0716304214276936E-4 80-81 0.36550207993259964 8.885788004844399E-4 0.0 1.755217383672968E-4 3.0716304214276936E-4 82-83 0.46469380232741825 9.324592350762643E-4 0.0 1.755217383672968E-4 3.0716304214276936E-4 84-85 0.5909049023221526 9.434293437242203E-4 0.0 1.755217383672968E-4 3.0716304214276936E-4 86-87 0.7417219560142524 9.434293437242203E-4 0.0 1.755217383672968E-4 3.1813315079072546E-4 88 0.8734400505502606 9.434293437242203E-4 0.0 1.755217383672968E-4 3.291032594386815E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGAGT 3030 0.0 84.775505 1 AACGAGT 86150 0.0 73.14344 1 ACGAGTG 90945 0.0 72.40835 2 GTGGAAT 96335 0.0 71.40238 6 CGAGTGG 93525 0.0 71.286705 3 TGGAATC 97810 0.0 70.782036 7 GAATCAC 98280 0.0 70.529594 9 GGAATCA 98475 0.0 70.37084 8 AGTGGAA 96450 0.0 69.670494 5 GAGTGGA 96875 0.0 69.4473 4 AACGTGT 1935 0.0 61.755146 1 CGTGTGG 2560 0.0 58.188126 3 ATCGAGT 1200 0.0 57.239 1 ACGTGTG 2515 0.0 53.466457 2 TCGAGTG 1510 0.0 51.687378 2 CGATTGG 830 0.0 46.424885 3 ACGATTG 800 0.0 44.66599 2 AGTACTT 46785 0.0 41.132145 20-21 GAGTACT 46040 0.0 41.0169 20-21 GTACTTT 46385 0.0 40.02885 22-23 >>END_MODULE