FastQCFastQC Report
Thu 26 May 2016
SRR2132675_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132675_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5834293
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2675924.586536877733086No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA488100.8366052236320665No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG348710.5976902428451913No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT108110.18530094391899754No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT69990.11996312149561224No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT33550.077.698811
AACGAGT952600.063.053231
ACGAGTG1004650.062.733362
GTGGAAT1049400.062.7023356
GAATCAC1064600.062.257339
TGGAATC1063100.062.2214057
GGAATCA1065150.062.2119528
CGAGTGG1032250.061.7910653
AGTGGAA1046650.061.560535
GAGTGGA1073700.060.1059074
ATCGAGT14400.052.609051
AACGTGT21500.050.3369031
CGTGTGG28650.046.4190753
TCGAGTG18800.044.52522
ACGTGTG27600.043.4485052
GAGTACT414750.040.13376220-21
AGTACTT428650.039.93963220-21
GTACTTT422350.038.72286622-23
TACTTTT464500.036.30174322-23
CATGGGG231200.035.70541824-25
Produced by FastQC (version 0.11.5)