FastQCFastQC Report
Thu 26 May 2016
SRR2132674_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132674_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6237194
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3857526.18470421154128No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA825131.322918607309633No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG180230.288960067620151No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT155000.24850918538047717No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT84840.13602270508180442No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT69760.11184516627188443No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT42850.082.241551
AACGAGT1017700.080.632831
ACGAGTG1086850.079.0931852
CGAGTGG1122050.077.651663
GTGGAAT1169300.076.917016
GAATCAC1182050.076.512719
TGGAATC1183250.076.3318567
GGAATCA1191950.075.8575068
GAGTGGA1163500.075.672844
AGTGGAA1171350.075.09355
ATCGAGT18100.059.5274351
TCGAGTG21000.055.9764942
AACGTGT26150.053.4373661
CGTGTGG34750.051.384863
ACGTGTG31600.050.591412
AGTACTT595600.041.55242520-21
GAGTACT582850.041.4656720-21
GTACTTT586000.040.51080722-23
TGAGTAC1189150.038.7869518-19
ATCACTG1209550.038.70549810-11
Produced by FastQC (version 0.11.5)