##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132674_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6237194
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.168133298403095	31.0	28.0	34.0	16.0	34.0
2	30.28906299852145	33.0	30.0	34.0	19.0	34.0
3	30.745967818220823	33.0	31.0	34.0	25.0	34.0
4	35.196739431225005	37.0	35.0	37.0	32.0	37.0
5	34.68078321758149	37.0	35.0	37.0	32.0	37.0
6	35.22335925417744	37.0	35.0	37.0	32.0	37.0
7	35.640286962374425	37.0	35.0	37.0	33.0	37.0
8	35.843773818803776	37.0	35.0	37.0	35.0	37.0
9	37.762423775819705	39.0	38.0	39.0	35.0	39.0
10-11	37.86242475382359	39.0	39.0	39.0	35.0	39.0
12-13	37.87776258041677	39.0	39.0	39.0	35.0	39.0
14-15	39.31044609162389	41.0	39.0	41.0	36.0	41.0
16-17	39.27004883926971	41.0	39.0	41.0	36.0	41.0
18-19	39.23464429677833	41.0	39.0	41.0	36.0	41.0
20-21	39.11843683233197	41.0	39.0	41.0	36.0	41.0
22-23	39.04238724977931	40.5	39.0	41.0	36.0	41.0
24-25	38.99409165403545	41.0	39.0	41.0	35.0	41.0
26-27	38.93435100784103	41.0	39.0	41.0	35.0	41.0
28-29	38.75989972413877	41.0	39.0	41.0	35.0	41.0
30-31	38.648361346464455	41.0	39.0	41.0	35.0	41.0
32-33	38.51684074601495	40.0	38.5	41.0	35.0	41.0
34-35	38.43741624839632	40.0	38.0	41.0	35.0	41.0
36-37	38.30938311362449	40.0	38.0	41.0	34.5	41.0
38-39	37.91670877962109	40.0	37.0	41.0	34.0	41.0
40-41	37.717603540951266	40.0	36.5	41.0	33.0	41.0
42-43	37.58280550516787	40.0	36.0	41.0	33.0	41.0
44-45	37.45215548850973	40.0	35.5	41.0	33.0	41.0
46-47	37.31708497442921	40.0	35.0	41.0	33.0	41.0
48-49	37.16980400160713	40.0	35.0	41.0	33.0	41.0
50-51	36.92668570193584	40.0	35.0	41.0	32.5	41.0
52-53	36.49621127705824	39.5	35.0	41.0	31.0	41.0
54-55	35.776191505346794	39.0	35.0	41.0	29.0	41.0
56-57	35.16951204339644	39.0	35.0	41.0	25.5	41.0
58-59	34.83971734725583	39.0	35.0	41.0	24.0	41.0
60-61	34.58506108676434	38.5	35.0	40.5	23.5	41.0
62-63	34.27569745305341	38.0	35.0	40.0	22.5	41.0
64-65	34.00051898337618	37.0	34.0	40.0	22.0	41.0
66-67	33.69505309599156	37.0	34.0	40.0	21.5	41.0
68-69	33.381225034847404	36.0	34.0	39.5	20.5	41.0
70-71	33.03141837499363	36.0	34.0	39.0	20.0	41.0
72-73	32.676848114713124	35.0	33.5	39.0	18.5	40.5
74-75	32.315256668303086	35.0	33.0	37.5	17.5	40.0
76-77	31.93970846505656	35.0	33.0	37.0	13.0	39.0
78-79	31.83261487457341	35.0	33.0	37.0	12.0	39.0
80-81	31.699723625720154	35.0	34.0	36.0	8.5	39.0
82-83	31.405916506685536	35.0	33.0	36.0	4.5	37.5
84-85	31.144477869375237	35.0	33.0	36.0	2.0	37.0
86-87	30.95054482833146	35.0	33.0	35.0	2.0	37.0
88-89	30.74674613295658	35.0	33.0	35.0	2.0	36.0
90-91	30.56144357542831	35.0	33.0	35.0	2.0	36.0
92-93	30.403258019551743	35.0	33.0	35.0	2.0	36.0
94-95	30.245851980874733	35.0	33.0	35.0	2.0	36.0
96-97	30.08836008628239	35.0	33.0	35.0	2.0	35.5
98-99	29.942840642763397	35.0	33.0	35.0	2.0	35.0
100	29.831267553967376	35.0	33.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	2.8512025032622184
1101	2	2.3234070775980626
1101	3	2.0930175598786676
1101	4	1.0519974177525384
1101	5	1.6448804177885563
1101	6	1.072362576556678
1101	7	0.6143786407101572
1101	8	0.477487472989786
1101	9	0.4077399490773814
1101	10-11	0.3864984822931561
1101	12-13	0.31305250005540586
1101	14-15	0.4241154093004269
1101	16-17	0.39740793860018897
1101	18-19	0.4497045136475748
1101	20-21	0.4711299856208342
1101	22-23	0.4060314915205012
1101	24-25	0.44486254525147473
1101	26-27	0.4146635717276155
1101	28-29	0.42328426455977564
1101	30-31	0.42962709985111047
1101	32-33	0.43661922206006665
1101	34-35	0.4553981702093708
1101	36-37	0.48344240940229355
1101	38-39	0.40654487814320817
1101	40-41	0.44585393183511
1101	42-43	0.4051756088050169
1101	44-45	0.4071635875015076
1101	46-47	0.39794514908531653
1101	48-49	0.3404879924892015
1101	50-51	0.40341692511927363
1101	52-53	0.3973228364086623
1101	54-55	0.43540704680275155
1101	56-57	0.31811417281680576
1101	58-59	0.30089705078437134
1101	60-61	0.2901421661391481
1101	62-63	0.2744385394401547
1101	64-65	0.3436180852773205
1101	66-67	0.2782423741412572
1101	68-69	0.30680687812417773
1101	70-71	0.3520061793991829
1101	72-73	0.3046715869641261
1101	74-75	0.3111930276841761
1101	76-77	0.24968045355591784
1101	78-79	0.2802164994903684
1101	80-81	0.30940772490495405
1101	82-83	0.3849058282666995
1101	84-85	0.3969088170249826
1101	86-87	0.32518094138553266
1101	88-89	0.3029235676283548
1101	90-91	0.27026454531387856
1101	92-93	0.2984022428725446
1101	94-95	0.37208538470686037
1101	96-97	0.38812427740202793
1101	98-99	0.35988344989855037
1101	100	0.3703224850493214
1102	1	2.7939559828708873
1102	2	2.2676553056619753
1102	3	2.0863262623392984
1102	4	1.0572381523435368
1102	5	1.5924712933424416
1102	6	1.0317738255197781
1102	7	0.4995665750099221
1102	8	0.42404985444966314
1102	9	0.42163103875034125
1102	10-11	0.3478281325745911
1102	12-13	0.31980888090112103
1102	14-15	0.39666803938020223
1102	16-17	0.34015297016225077
1102	18-19	0.3829738937860441
1102	20-21	0.37526622148806865
1102	22-23	0.3372440502087244
1102	24-25	0.3849551402885254
1102	26-27	0.3242880921828615
1102	28-29	0.432242695446277
1102	30-31	0.42506993450422215
1102	32-33	0.4196403435660443
1102	34-35	0.4192879120854158
1102	36-37	0.43525225054288796
1102	38-39	0.3760078019176518
1102	40-41	0.3794097952379829
1102	42-43	0.40232714425137317
1102	44-45	0.4482369062638156
1102	46-47	0.4160929148687771
1102	48-49	0.43820051713512953
1102	50-51	0.4430586890380539
1102	52-53	0.3586480403497845
1102	54-55	0.3314582797590191
1102	56-57	0.2709342224768676
1102	58-59	0.2733018122590565
1102	60-61	0.26417170373365195
1102	62-63	0.2548997360892784
1102	64-65	0.33383527510687117
1102	66-67	0.23209761519542838
1102	68-69	0.2182406700326709
1102	70-71	0.2786775517760134
1102	72-73	0.253373658541868
1102	74-75	0.2813993239799615
1102	76-77	0.198121636115701
1102	78-79	0.23239841594640964
1102	80-81	0.2827431609867297
1102	82-83	0.3406176387192126
1102	84-85	0.29072876525290425
1102	86-87	0.3061518275828412
1102	88-89	0.2886164394500881
1102	90-91	0.30161966478843283
1102	92-93	0.3468118286264712
1102	94-95	0.32375588865784977
1102	96-97	0.2747496023669811
1102	98-99	0.3090538044802962
1102	100	0.2648601837625222
1103	1	2.5601623780212392
1103	2	2.1738025768886047
1103	3	1.9998143171108609
1103	4	1.0318103860578347
1103	5	1.5737555999937172
1103	6	1.0442653930957917
1103	7	0.5553758664896691
1103	8	0.43883154547908987
1103	9	0.429526901113519
1103	10-11	0.30430748426525867
1103	12-13	0.23730153820577016
1103	14-15	0.384701480666763
1103	16-17	0.36037085034235616
1103	18-19	0.3348272791277509
1103	20-21	0.3948889022642206
1103	22-23	0.3604066557471626
1103	24-25	0.40399343701261614
1103	26-27	0.39393920422499207
1103	28-29	0.44475963540086383
1103	30-31	0.416847126238018
1103	32-33	0.4140410501690326
1103	34-35	0.40489117657375573
1103	36-37	0.3723797695503279
1103	38-39	0.3743858541820231
1103	40-41	0.32613544745012746
1103	42-43	0.3728818844070858
1103	44-45	0.3485738208624056
1103	46-47	0.3595447928344697
1103	48-49	0.3623705071555108
1103	50-51	0.40802702682225345
1103	52-53	0.3877013529336608
1103	54-55	0.38261636906869967
1103	56-57	0.39070725303133713
1103	58-59	0.34698852538075187
1103	60-61	0.3598010725486205
1103	62-63	0.3340321700733
1103	64-65	0.3227285368695263
1103	66-67	0.3089453334019794
1103	68-69	0.3654225061407601
1103	70-71	0.33965425998710685
1103	72-73	0.3576039905786317
1103	74-75	0.280142585551431
1103	76-77	0.22892422262349044
1103	78-79	0.23476342751829193
1103	80-81	0.26361593803589756
1103	82-83	0.2927585594770967
1103	84-85	0.3135619122308064
1103	86-87	0.3841491139127271
1103	88-89	0.35531307593026895
1103	90-91	0.3196340808877949
1103	92-93	0.31732513724472255
1103	94-95	0.34045927107722207
1103	96-97	0.3653170041316329
1103	98-99	0.3355826336747576
1103	100	0.3980464902160641
1104	1	1.5159331339812674
1104	2	1.739551025396949
1104	3	1.8070311565917905
1104	4	0.8831234242733288
1104	5	1.5238516087093643
1104	6	1.0274176509384247
1104	7	0.5226286599441252
1104	8	0.4252099569358876
1104	9	0.3646461272983359
1104	10-11	0.2731524930571041
1104	12-13	0.2418442781952308
1104	14-15	0.33470005926540125
1104	16-17	0.28618389459613525
1104	18-19	0.29512717557919643
1104	20-21	0.3500899488003668
1104	22-23	0.2743317936364562
1104	24-25	0.30355461941763906
1104	26-27	0.3111289537195674
1104	28-29	0.40770974538847327
1104	30-31	0.4017724112904446
1104	32-33	0.34720047379826013
1104	34-35	0.28680020517630567
1104	36-37	0.3478716038132177
1104	38-39	0.3740091240399863
1104	40-41	0.3137919231677344
1104	42-43	0.30305694761936763
1104	44-45	0.3208707402795312
1104	46-47	0.3636169581299882
1104	48-49	0.32621031821386026
1104	50-51	0.30848710665798507
1104	52-53	0.3307244043233055
1104	54-55	0.31114463192493247
1104	56-57	0.26091213681680614
1104	58-59	0.28565529685350555
1104	60-61	0.27839091307700414
1104	62-63	0.2864749445076953
1104	64-65	0.2972589427287744
1104	66-67	0.33214899467541414
1104	68-69	0.2800457498150166
1104	70-71	0.2334075483136715
1104	72-73	0.2520221580973043
1104	74-75	0.22691638918836077
1104	76-77	0.32978123680641147
1104	78-79	0.29717984404146947
1104	80-81	0.28114391158885255
1104	82-83	0.26627935890742194
1104	84-85	0.23864789899809224
1104	86-87	0.19409375620745806
1104	88-89	0.2451458774493105
1104	90-91	0.2092591921552014
1104	92-93	0.23342929411699131
1104	94-95	0.19853710039000205
1104	96-97	0.24726340597162988
1104	98-99	0.2972428700142622
1104	100	0.25731327367988754
1105	1	2.208978479747291
1105	2	2.025087963017583
1105	3	1.97653596797997
1105	4	0.9993017312934995
1105	5	1.5695694477011202
1105	6	1.0672354565014146
1105	7	0.590196780336484
1105	8	0.4169560558308092
1105	9	0.3755704585352433
1105	10-11	0.30789595418062987
1105	12-13	0.2599843166501401
1105	14-15	0.357015621265667
1105	16-17	0.2870352321596883
1105	18-19	0.305620675110724
1105	20-21	0.41190404013016746
1105	22-23	0.36875776951618633
1105	24-25	0.4050061616384397
1105	26-27	0.28986868811511357
1105	28-29	0.3291901715464647
1105	30-31	0.3718914590725646
1105	32-33	0.36856865228934055
1105	34-35	0.36428945664390966
1105	36-37	0.3543974562269483
1105	38-39	0.32598522760115856
1105	40-41	0.2667759591381227
1105	42-43	0.3212781503466573
1105	44-45	0.37531256586773054
1105	46-47	0.3566825861430303
1105	48-49	0.31035086505330156
1105	50-51	0.3192205290654968
1105	52-53	0.2837574854735081
1105	54-55	0.23933396337997692
1105	56-57	0.17008105015951713
1105	58-59	0.11549950047082547
1105	60-61	0.22633215811390528
1105	62-63	0.23985719495957625
1105	64-65	0.19655745655812495
1105	66-67	0.2297888609221701
1105	68-69	0.13013669302792152
1105	70-71	0.07064630904928038
1105	72-73	0.16606615816156278
1105	74-75	0.18279608329255836
1105	76-77	0.13035992945494002
1105	78-79	0.12379559110167904
1105	80-81	0.1757714843281697
1105	82-83	0.19055666790903203
1105	84-85	0.20911909449209887
1105	86-87	0.16692993393290578
1105	88-89	0.17845367947681012
1105	90-91	0.21886457023832762
1105	92-93	0.26481774738311614
1105	94-95	0.24718615682757417
1105	96-97	0.2506624725067397
1105	98-99	0.22014510825527722
1105	100	0.22541767602753282
1106	1	1.7420032362002544
1106	2	1.1495149353786864
1106	3	1.5906638126251238
1106	4	0.8470616909644875
1106	5	1.529862372611305
1106	6	1.0380145183338527
1106	7	0.546573947765765
1106	8	0.4463181886429055
1106	9	0.3916609758297298
1106	10-11	0.3159482115688945
1106	12-13	0.24824513062496578
1106	14-15	0.3440531345988518
1106	16-17	0.31186825958145903
1106	18-19	0.308150905504192
1106	20-21	0.35575793382306387
1106	22-23	0.30445801810414963
1106	24-25	0.3585580638749022
1106	26-27	0.3057422800161973
1106	28-29	0.3360945642511197
1106	30-31	0.2859743501484999
1106	32-33	0.2871646995549213
1106	34-35	0.2787388540487541
1106	36-37	0.22405861632280732
1106	38-39	0.20878750236174426
1106	40-41	0.24918343679836852
1106	42-43	0.23384317358085127
1106	44-45	0.24516849630099813
1106	46-47	0.21634536616534916
1106	48-49	0.23736330754417878
1106	50-51	0.106284794158654
1106	52-53	0.13867335542219905
1106	54-55	0.07439379006167712
1106	56-57	-0.024380127900286652
1106	58-59	0.026974961532843622
1106	60-61	0.03052298288946531
1106	62-63	0.01935800652890407
1106	64-65	-0.03165718056716571
1106	66-67	0.02149595967657092
1106	68-69	0.055985885803195856
1106	70-71	0.031919459227651714
1106	72-73	-0.024912421970448406
1106	74-75	0.03062534422265628
1106	76-77	-0.02934532789359423
1106	78-79	-0.0037669138055740348
1106	80-81	0.033673102583474446
1106	82-83	0.0642120455911872
1106	84-85	0.047989794108818984
1106	86-87	0.03722856904321148
1106	88-89	0.024523654119512628
1106	90-91	0.07317636294108709
1106	92-93	0.11258526488119358
1106	94-95	0.14104139537658966
1106	96-97	0.11572655759451322
1106	98-99	0.12533657917168384
1106	100	0.1375029983349556
1107	1	1.6426210556792853
1107	2	1.0488859675091042
1107	3	1.403994045026856
1107	4	0.7348724142178753
1107	5	1.4413883411247355
1107	6	1.00582475588309
1107	7	0.544776772397455
1107	8	0.4552481034845428
1107	9	0.4284544354512718
1107	10-11	0.2695807489938957
1107	12-13	0.22225816323955172
1107	14-15	0.3225188920112174
1107	16-17	0.29911729039113055
1107	18-19	0.28845979814003897
1107	20-21	0.256703353549419
1107	22-23	0.2871873897633179
1107	24-25	0.373096826421758
1107	26-27	0.28894541326406653
1107	28-29	0.315173468140479
1107	30-31	0.23455481425741453
1107	32-33	0.23244732650861977
1107	34-35	0.1822508841333672
1107	36-37	0.25415349456755365
1107	38-39	0.25373730594813537
1107	40-41	0.269803583376401
1107	42-43	0.23980629225238914
1107	44-45	0.23001689234256162
1107	46-47	0.2612583068472176
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2210	2	1.7104314949087467
2210	3	1.8137073993249473
2210	4	0.9455330701383247
2210	5	1.577284137254125
2210	6	1.041311959597543
2210	7	0.5907902450496039
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2210	14-15	0.30596761244802906
2210	16-17	0.32230159722466567
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2210	20-21	0.30963552458604937
2210	22-23	0.2931351005241254
2210	24-25	0.3369530400984644
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2211	84-85	-0.3259183587633636
2211	86-87	-0.17570541067757972
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2212	3	-0.691411316776712
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2212	58-59	0.06873233143581103
2212	60-61	0.06702291293304086
2212	62-63	-1.2946433920927518
2212	64-65	-0.21672998361971452
2212	66-67	0.06771500849282575
2212	68-69	0.1132888149605833
2212	70-71	0.14324797217618368
2212	72-73	0.11075654432119819
2212	74-75	0.15296530126935437
2212	76-77	0.23622872012399654
2212	78-79	0.19283990422608
2212	80-81	0.20213019686916311
2212	82-83	0.22440515258598026
2212	84-85	0.2439412463526942
2212	86-87	0.2591858602484933
2212	88-89	0.2719406574976233
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2212	94-95	0.2700424320606345
2212	96-97	0.24118519988578413
2212	98-99	0.24710290723772843
2212	100	0.2558284294692079
2213	1	-2.8090526026514766
2213	2	-2.9488278922577216
2213	3	-2.683072683530927
2213	4	-1.5438120819251182
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2213	7	-0.904723789750733
2213	8	-0.9497024810054029
2213	9	-1.0445846889942416
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2213	30-31	-0.5316784670194394
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2213	34-35	-0.5254723074901122
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2213	66-67	-0.649206999720775
2213	68-69	-0.6832012365981086
2213	70-71	-0.7089449269934818
2213	72-73	-0.6962375933633922
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2213	80-81	-0.6464968314207304
2213	82-83	-0.5852357089546452
2213	84-85	-0.6215320069774926
2213	86-87	-0.6550729991727913
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2213	96-97	-0.6396908469862943
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2213	100	-0.6430929684182516
2214	1	-2.625646009279805
2214	2	-2.578058691249744
2214	3	-2.7054392993568825
2214	4	-1.423254323298572
2214	5	-2.151431264670279
2214	6	-1.2828302323879086
2214	7	-0.7582259079680398
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2214	16-17	-0.6238627509634966
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2214	82-83	-0.40941155239147164
2214	84-85	-0.3889382026086814
2214	86-87	-0.38895373510349884
2214	88-89	-0.3762857477433741
2214	90-91	-0.4071208129958883
2214	92-93	-0.48207131571018635
2214	94-95	-0.4760029162086745
2214	96-97	-0.49823658398334913
2214	98-99	-0.5855298028719282
2214	100	-0.6033843533388392
2215	1	-5.381553660702668
2215	2	-4.251769899441907
2215	3	-3.538805982548652
2215	4	-1.8134672826145888
2215	5	-2.6685543009854342
2215	6	-5.000919952628205
2215	7	-2.166100712454906
2215	8	-1.2442090042366942
2215	9	-1.6557183022545843
2215	10-11	-0.8808930490635163
2215	12-13	-0.6833954791956245
2215	14-15	-0.7685860381508647
2215	16-17	-0.6415879772938666
2215	18-19	-0.7249085575077743
2215	20-21	-0.7260213173188035
2215	22-23	-0.7614120979931585
2215	24-25	-0.8872848603569707
2215	26-27	-0.846218816951712
2215	28-29	-0.8505328224650626
2215	30-31	-0.9892420373715538
2215	32-33	-0.8925506331311297
2215	34-35	-0.8957468863881601
2215	36-37	-0.8351719076255648
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2215	44-45	-0.9035208942224244
2215	46-47	-0.9289412255370948
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2215	54-55	-0.9451702622930256
2215	56-57	-0.9089575704443078
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2215	62-63	-0.7879329215894515
2215	64-65	-0.836659200949164
2215	66-67	-0.7604048035701396
2215	68-69	-0.7906641439316928
2215	70-71	-0.8206172312245883
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2215	78-79	-0.6403611385836676
2215	80-81	-0.6498629621236951
2215	82-83	-0.6212630978262119
2215	84-85	-0.6247613207160931
2215	86-87	-0.6059599923715098
2215	88-89	-0.6294522921589873
2215	90-91	-0.5979069351360558
2215	92-93	-0.6230313594977979
2215	94-95	-0.6221666221931912
2215	96-97	-0.6996602874685038
2215	98-99	-0.6648034185096101
2215	100	-0.6984007450078309
2216	1	-5.740282306946309
2216	2	-4.715800365598618
2216	3	-5.750444819016831
2216	4	-3.0385853498966426
2216	5	-4.108278362685155
2216	6	-1.9324741194317951
2216	7	-1.2748065621944065
2216	8	-0.8426669578390147
2216	9	-0.7402317136377476
2216	10-11	-1.065831563525741
2216	12-13	-0.8647383692029678
2216	14-15	-1.7847973246239874
2216	16-17	-1.3119937449965988
2216	18-19	-1.8185273961928772
2216	20-21	-1.2347420611392366
2216	22-23	-0.9238926952398927
2216	24-25	-1.6068985543554817
2216	26-27	-1.3794240406822453
2216	28-29	-2.1750981076354705
2216	30-31	-2.219185908382812
2216	32-33	-1.3618975615077176
2216	34-35	-1.3621881499062667
2216	36-37	-1.4143971266433226
2216	38-39	-1.2851653831925631
2216	40-41	-1.2696104130081522
2216	42-43	-1.6356635478115251
2216	44-45	-1.5137740929414036
2216	46-47	-1.9053632692760303
2216	48-49	-1.4869716238606756
2216	50-51	-1.4167771788373287
2216	52-53	-1.2882345425869914
2216	54-55	-1.2096013415700568
2216	56-57	-1.1561007442908533
2216	58-59	-1.1386069741540155
2216	60-61	-1.1493421993215946
2216	62-63	-1.081740194590509
2216	64-65	-1.169383489048542
2216	66-67	-1.2003737867506032
2216	68-69	-1.171383480312926
2216	70-71	-1.1249542888517183
2216	72-73	-1.113689697451207
2216	74-75	-1.105244078099311
2216	76-77	-1.094227898209386
2216	78-79	-1.0970827608987186
2216	80-81	-1.066904868918101
2216	82-83	-1.0512051659158708
2216	84-85	-1.0189418313284513
2216	86-87	-1.0832223705339104
2216	88-89	-1.0905592284417267
2216	90-91	-1.1338925168425256
2216	92-93	-1.2075041586214823
2216	94-95	-1.2619375883159343
2216	96-97	-1.2779272651499127
2216	98-99	-1.2559526541573334
2216	100	-1.3599685954061904
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	9279.0
3	4370.0
4	4716.0
5	6144.0
6	5759.0
7	4838.0
8	4949.0
9	5699.0
10	6662.0
11	13914.0
12	9874.0
13	13861.0
14	12588.0
15	13115.0
16	15527.0
17	19300.0
18	26268.0
19	43354.0
20	90015.0
21	120220.0
22	59124.0
23	39691.0
24	40479.0
25	44795.0
26	45277.0
27	49426.0
28	57040.0
29	66716.0
30	79661.0
31	98057.0
32	125742.0
33	171365.0
34	249008.0
35	456885.0
36	723178.0
37	1283181.0
38	1652985.0
39	560984.0
40	3148.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	15.57759213844353	40.10680723250105	26.506732282308754	17.80886834674667
2	18.658227510982258	37.97259076635378	25.261731532876013	18.10745018978795
3	19.789363614471508	24.678324900588308	21.465389724930795	34.066921760009386
4	32.02975568821492	25.663784067001927	23.006643692660514	19.299816552122635
5	18.505789622705336	36.823545972756335	24.14645111247141	20.524213292066914
6	34.91677507545861	23.12171146191701	24.1107299211793	17.85078354144508
7	19.71070644908592	23.248819902026458	37.85503545344269	19.18543819544494
8	33.813682883681345	23.40193362592217	23.928500540467397	18.855882949929086
9	33.546607657225344	24.946778952201903	23.632518084253913	17.874095306318836
10-11	17.939429172797897	40.0019704373473	23.707880498826878	18.350719891027918
12-13	17.916774754801597	24.71446294599783	31.143956721564216	26.224805577636356
14-15	18.16659061751166	32.06291963982522	23.673377804185662	26.097111938477465
16-17	25.98638408737913	24.392139886767875	31.19821233528734	18.42326369056566
18-19	18.398169112584924	24.534446098678348	31.23475877133211	25.83262601740462
20-21	26.1150514235829	32.25100175680707	23.53513032959272	18.098816490017303
22-23	25.785812664502178	24.732148991144168	31.245843981491983	18.23619436286167
24-25	18.28044790654259	32.15974202501958	23.566406624517374	25.993403443920453
26-27	18.554898609987337	27.835622711099887	35.36095187620966	18.248526802703115
28-29	25.52031501642982	24.48712429665223	31.801564340037537	18.19099634688042
30-31	23.719735412666722	25.594492950839502	32.065977996912366	18.61979363958141
32-33	20.636795232148494	26.59042886389475	33.01667744356478	19.756098460391975
34-35	20.27941850807913	26.389605038271235	33.385353034923504	19.94562341872613
36-37	20.197233782135633	26.52459978701913	33.45610638813522	19.822060042710017
38-39	20.666489054695695	26.495406074120954	33.114364978810436	19.72373989237292
40-41	20.141749567960353	27.070216853959337	32.99186762579455	19.79616595228576
42-43	20.341110702240055	26.622781173010985	33.19451760029567	19.841590524453295
44-45	20.166264783814977	26.80659495651785	32.97792507559002	20.04921518407715
46-47	20.398026182882553	26.57243357919735	33.118619180984865	19.910921056935234
48-49	20.09532984451681	26.879169738734788	32.96884315661603	20.056657260132376
50-51	20.4529975824041	27.03707608391956	32.50214160273259	20.007784730943744
52-53	20.59503345931851	27.054925807807212	32.27197246358292	20.078068269291357
54-55	20.77399826554202	27.70170529381651	31.07218619145062	20.45211024919085
56-57	21.087739522266162	28.326697027731395	30.02215753817245	20.563405911829992
58-59	21.586665357305336	28.751209990159747	29.143353020516223	20.518771632018694
60-61	21.423682815368437	29.032516933974033	28.854757406306263	20.689042844351263
62-63	21.01034249504135	29.095580417961408	28.868022118673103	21.026054968324136
64-65	21.06354154791379	28.994942991403615	29.233154247981822	20.708361212700773
66-67	20.94564242501452	28.66380538862362	29.59972439627704	20.790827790084823
68-69	21.16759353181642	28.524850900370307	29.677921709746307	20.629633858066963
70-71	20.645829927678932	28.906611965610235	29.826052468470422	20.62150563824041
72-73	20.60603441793333	28.476197317170666	29.966916378477016	20.95085188641899
74-75	20.74681398801376	28.73461306381355	29.526723972969034	20.991848975203656
76-77	20.973188057182544	28.617253738173115	29.576599990684482	20.83295821395986
78-79	21.008860708545598	28.983394169157638	29.051264210816136	20.95648091148063
80-81	21.16313783886251	29.38250317016869	28.692157198678043	20.762201792290753
82-83	21.16192216252704	29.418894613444202	28.735464749223453	20.6837184748053
84-85	21.100209448109123	29.580092055835085	28.3131658892197	21.006532606836092
86-87	21.137999615825056	29.626165392123006	28.303014618850675	20.932820373201263
88-89	21.55884558258996	29.515188523455333	28.062141795894917	20.86382409805979
90-91	21.377940938791532	29.81769129730964	27.93861387535627	20.865753888542557
92-93	21.129889012222005	29.910675022318216	27.835527874419896	21.123908091039883
94-95	21.158871965097934	30.094268542523867	27.69396588008532	21.05289361229288
96-97	21.156470286029297	29.80978575462431	27.812589894257894	21.221154065088495
98-99	21.53194724658134	29.87343146136827	27.324199696475365	21.27042159557503
100	21.235459826231395	30.301649984046897	27.34157122861362	21.12131896110809
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	81596.0
1	44941.5
2	6369.0
3	3864.0
4	3171.0
5	3003.0
6	3115.5
7	3436.0
8	3239.0
9	3022.0
10	3312.0
11	5041.5
12	43038.0
13	49891.5
14	18972.5
15	18683.0
16	20905.5
17	23270.0
18	25459.5
19	27599.5
20	30201.0
21	33291.0
22	37290.5
23	42491.0
24	47962.0
25	53929.5
26	62001.0
27	70241.0
28	77334.0
29	84932.0
30	94032.0
31	103302.0
32	112526.5
33	123623.5
34	135345.5
35	144653.0
36	154214.0
37	163078.5
38	170007.0
39	175706.5
40	179270.0
41	183053.5
42	186801.5
43	190960.0
44	195764.5
45	199035.5
46	201116.0
47	202974.5
48	201800.0
49	201577.0
50	216385.0
51	210890.5
52	183030.0
53	171258.0
54	165886.0
55	159112.5
56	149127.5
57	137338.5
58	125048.0
59	107298.5
60	87143.5
61	71089.0
62	56372.5
63	43948.5
64	35010.5
65	27052.0
66	20587.0
67	16097.5
68	12201.5
69	9157.0
70	6964.0
71	5042.0
72	3551.0
73	2452.5
74	1754.0
75	1335.0
76	903.5
77	564.0
78	373.0
79	224.0
80	129.0
81	82.0
82	48.0
83	30.5
84	19.5
85	12.0
86	11.0
87	8.5
88	4.0
89	2.5
90	2.5
91	1.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.059658237341984235
2	0.06709748005272884
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	1.0421352935310333E-4
18-19	0.0
20-21	4.248705427472674E-4
22-23	7.214782801368692E-5
24-25	0.0
26-27	0.002637403935167
28-29	9.459381895127842E-4
30-31	0.00621272963451193
32-33	0.001394858008264614
34-35	0.0021484019897409
36-37	0.007751883298803917
38-39	0.012064720128955425
40-41	0.003342849364634161
42-43	0.008160720990881477
44-45	0.0015151043882874254
46-47	0.002276664795098565
48-49	0.0034871450206615346
50-51	0.01004458094457219
52-53	0.003479128595326681
54-55	0.001394858008264614
56-57	6.2528117611862E-4
58-59	9.058560628385136E-4
60-61	0.0063730581412090115
62-63	0.001779646424337611
64-65	0.008064523886863227
66-67	0.011279110446139723
68-69	0.012184966508978236
70-71	0.010092679496581314
72-73	0.004344902531490924
74-75	0.00201212275904838
76-77	0.0046575431195502335
78-79	0.016209212027074996
80-81	0.00863369008563787
82-83	0.006677682303933467
84-85	0.005242742168994583
86-87	0.007246848502708109
88-89	0.004240689002137821
90-91	0.006573468774580364
92-93	0.011166880491451765
94-95	0.008705837913651555
96-97	0.011222995468795743
98-99	0.0022205498177545863
100	0.0026293875098321456
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6237194.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.55275125325473
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.6546519701333	48.83861078165313
2	13.119348183575575	15.88825254329782
3	3.1956286332980492	5.805123171896255
4	1.0018461994746706	2.426581748432334
5	0.47250833212934507	1.430583975025925
6	0.2795723630722941	1.0157325455040747
7	0.19238177977007612	0.8154472239253096
8	0.14152016945598325	0.6855548494709289
9	0.12102130515488964	0.659535568864944
>10	0.7491025122756387	8.811476822393299
>50	0.06006399123417457	2.244208996643648
>100	0.008918365239756528	0.9697603292145285
>500	0.002104350892351648	0.8567878955663198
>1k	0.0011720229787254798	1.2094606028369157
>5k	5.327377172620124E-5	0.2493600175040669
>10k+	1.0654754345240249E-4	8.093522927770422
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	385752	6.18470421154128	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	82513	1.322918607309633	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	18023	0.288960067620151	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	15500	0.24850918538047717	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8484	0.13602270508180442	No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6976	0.11184516627188443	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.006717764430607738	1.6032850669708206E-5	0.0	3.206570133941641E-5	0.0
2	0.006733797281277446	1.6032850669708206E-5	0.0	3.206570133941641E-5	0.0
3	0.006733797281277446	1.6032850669708206E-5	0.0	3.206570133941641E-5	0.0
4	0.006765862982616863	3.206570133941641E-5	0.0	4.8098552009124614E-5	0.0
5	0.006765862982616863	3.206570133941641E-5	0.0	8.016425334854103E-5	0.0
6	0.006765862982616863	3.206570133941641E-5	0.0	8.016425334854103E-5	0.0
7	0.006781895833286571	3.206570133941641E-5	0.0	8.016425334854103E-5	0.0
8	0.006797928683956279	3.206570133941641E-5	0.0	8.016425334854103E-5	0.0
9	0.006829994385295695	3.206570133941641E-5	0.0	1.1222995468795743E-4	0.0
10-11	0.006846027235965404	4.008212667427051E-5	0.0	1.1222995468795743E-4	0.0
12-13	0.00687809293730482	4.8098552009124614E-5	0.0	1.3627923069251975E-4	0.0
14-15	0.006886109362639675	4.8098552009124614E-5	0.0	1.4429565602737385E-4	0.0
16-17	0.006894125787974529	4.8098552009124614E-5	0.0	1.4429565602737385E-4	0.0
18-19	0.006942224339983653	8.016425334854103E-5	0.0	1.5231208136222795E-4	0.0
20-21	0.007046437869336756	9.619710401824923E-5	0.0	1.7636135736679025E-4	0.0
22-23	0.00709453642134588	9.619710401824923E-5	0.0	1.9239420803649846E-4	0.0
24-25	0.0071586678240247135	9.619710401824923E-5	0.0	1.9239420803649846E-4	0.0
26-27	0.007254864928042963	9.619710401824923E-5	0.0	1.9239420803649846E-4	0.0
28-29	0.007367094882730921	9.619710401824923E-5	0.0	2.1644348404106076E-4	0.0
30-31	0.0074713084120840234	9.619710401824923E-5	0.0	2.485091853804772E-4	0.0
32-33	0.007543456240097711	1.2024638002281155E-4	0.0	2.805748867198936E-4	0.0
34-35	0.007703784746794793	1.2826280535766565E-4	0.0	3.286734387290182E-4	0.0
36-37	0.008048491036193518	1.4429565602737385E-4	0.0	3.447062893987264E-4	0.0
38-39	0.008585591533628744	1.6032850669708205E-4	0.0	3.607391400684346E-4	1.6032850669708206E-5
40-41	0.009178807008407947	1.6032850669708205E-4	0.0	3.767719907381428E-4	1.6032850669708206E-5
42-43	0.009932350989884233	1.7636135736679025E-4	0.0	3.847884160729969E-4	1.6032850669708206E-5
44-45	0.010886305604731871	1.8437778270164436E-4	0.0	3.92804841407851E-4	1.6032850669708206E-5
46-47	0.011808194518240094	2.0842705870620666E-4	0.0	4.008212667427051E-4	1.6032850669708206E-5
48-49	0.013018674743803062	2.2445990937591486E-4	0.0	4.088376920775592E-4	1.6032850669708206E-5
50-51	0.014678074788117862	2.2445990937591486E-4	0.0	4.168541174124133E-4	1.6032850669708206E-5
52-53	0.016425655511116057	2.2445990937591486E-4	0.0	4.328869680821215E-4	1.6032850669708206E-5
54-55	0.019006944468939076	2.2445990937591486E-4	0.0	4.409033934169756E-4	1.6032850669708206E-5
56-57	0.022373843109577803	2.404927600456231E-4	0.0	4.489198187518297E-4	1.6032850669708206E-5
58-59	0.02633395722499573	2.565256107153313E-4	0.0	4.729690947563921E-4	1.6032850669708206E-5
60-61	0.03164083079666914	2.725584613850395E-4	0.0	4.809855200912462E-4	1.6032850669708206E-5
62-63	0.038767432919354444	2.805748867198936E-4	0.0	4.809855200912462E-4	1.6032850669708206E-5
64-65	0.04817871626247316	2.885913120547477E-4	0.0	4.890019454261002E-4	1.6032850669708206E-5
66-67	0.05947385955928258	2.885913120547477E-4	0.0	5.130512214306626E-4	1.6032850669708206E-5
68-69	0.07468101841950081	2.966077373896018E-4	0.0	5.210676467655166E-4	1.6032850669708206E-5
70-71	0.09532331365675015	3.046241627244559E-4	0.0	5.37100497435225E-4	1.6032850669708206E-5
72-73	0.12207412499915828	3.046241627244559E-4	0.0	5.53133348104933E-4	1.6032850669708206E-5
74-75	0.15722615009249352	3.366898640638723E-4	0.0	5.611497734397872E-4	1.6032850669708206E-5
76-77	0.2014527686648836	3.527227147335805E-4	0.0	5.932154747792036E-4	1.6032850669708206E-5
78-79	0.2580487315289536	3.607391400684346E-4	0.0	5.932154747792036E-4	1.6032850669708206E-5
80-81	0.32879368510904106	3.847884160729969E-4	0.0	6.012319001140578E-4	1.6032850669708206E-5
82-83	0.4202129996277172	4.008212667427051E-4	0.0	6.2528117611862E-4	1.6032850669708206E-5
84-85	0.5327315456277294	4.008212667427051E-4	0.0	6.2528117611862E-4	1.6032850669708206E-5
86-87	0.6658923868649909	4.008212667427051E-4	0.0	6.332976014534742E-4	1.6032850669708206E-5
88	0.7818259300576509	4.008212667427051E-4	0.0	6.573468774580364E-4	1.6032850669708206E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	4285	0.0	82.24155	1
AACGAGT	101770	0.0	80.63283	1
ACGAGTG	108685	0.0	79.093185	2
CGAGTGG	112205	0.0	77.65166	3
GTGGAAT	116930	0.0	76.91701	6
GAATCAC	118205	0.0	76.51271	9
TGGAATC	118325	0.0	76.331856	7
GGAATCA	119195	0.0	75.857506	8
GAGTGGA	116350	0.0	75.67284	4
AGTGGAA	117135	0.0	75.0935	5
ATCGAGT	1810	0.0	59.527435	1
TCGAGTG	2100	0.0	55.976494	2
AACGTGT	2615	0.0	53.437366	1
CGTGTGG	3475	0.0	51.38486	3
ACGTGTG	3160	0.0	50.59141	2
AGTACTT	59560	0.0	41.552425	20-21
GAGTACT	58285	0.0	41.46567	20-21
GTACTTT	58600	0.0	40.510807	22-23
TGAGTAC	118915	0.0	38.78695	18-19
ATCACTG	120955	0.0	38.705498	10-11
>>END_MODULE