FastQCFastQC Report
Thu 26 May 2016
SRR2132673_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132673_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4023807
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2857187.100688477354902No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA960262.3864464672386125No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG276340.6867625609279968No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT111210.2763800550076085No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA55970.13909712866447121No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT54880.13638825122576703No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT52600.13072197548242248No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45770.11374800033898247No Hit
CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT41920.10417994700044012No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT28250.084.43121
AACGAGT869350.068.4394151
GTGGAAT955650.068.1925666
ACGAGTG912950.067.985912
GAATCAC971650.067.52919
TGGAATC973800.067.283497
CGAGTGG939950.067.041873
AGTGGAA950300.067.023765
GGAATCA980000.066.953738
GAGTGGA968150.065.807454
ATCGAGT12750.064.202581
AACGTGT17600.061.2119521
TCGAGTG14850.060.4782072
CGTGTGG23800.056.666583
ACGTGTG24150.050.0389252
GAGTACT428850.041.50853720-21
AGTACTT437450.041.4068820-21
CGATTGG8350.041.082653
GTACTTT433400.040.298522-23
TACTTTT471800.037.81045522-23
Produced by FastQC (version 0.11.5)