##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132673_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4023807
Sequences flagged as poor quality	0
Sequence length	100
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.223185406258303	31.0	28.0	34.0	16.0	34.0
2	30.34185188305503	33.0	30.0	34.0	23.0	34.0
3	30.79316279334471	33.0	31.0	34.0	25.0	34.0
4	35.25765450480105	37.0	35.0	37.0	32.0	37.0
5	34.70586387468385	37.0	35.0	37.0	32.0	37.0
6	35.26294601107856	37.0	35.0	37.0	32.0	37.0
7	35.672882670565464	37.0	35.0	37.0	35.0	37.0
8	35.881021629516525	37.0	35.0	37.0	35.0	37.0
9	37.80272165141121	39.0	39.0	39.0	35.0	39.0
10-11	37.89476073280851	39.0	39.0	39.0	35.0	39.0
12-13	37.90278112245443	39.0	39.0	39.0	35.0	39.0
14-15	39.33884577466067	41.0	39.5	41.0	37.0	41.0
16-17	39.290603028425565	41.0	39.0	41.0	36.5	41.0
18-19	39.20329193224228	41.0	39.0	41.0	36.0	41.0
20-21	39.06367974408315	41.0	39.0	41.0	35.0	41.0
22-23	38.982362101363215	40.5	39.0	41.0	35.0	41.0
24-25	38.9156875565851	41.0	39.0	41.0	35.0	41.0
26-27	38.86233944620108	41.0	39.0	41.0	35.0	41.0
28-29	38.6895961958414	41.0	39.0	41.0	35.0	41.0
30-31	38.566724994513905	41.0	38.5	41.0	35.0	41.0
32-33	38.43129839477888	40.0	38.0	41.0	35.0	41.0
34-35	38.352967103044456	40.0	38.0	41.0	35.0	41.0
36-37	38.19568694522377	40.0	38.0	41.0	34.0	41.0
38-39	37.75856098465955	40.0	36.5	41.0	34.0	41.0
40-41	37.55971695461537	40.0	35.0	41.0	33.0	41.0
42-43	37.425324450203505	40.0	35.0	41.0	33.0	41.0
44-45	37.28451898910659	40.0	35.0	41.0	33.0	41.0
46-47	37.144289972158205	40.0	35.0	41.0	33.0	41.0
48-49	36.98644430510708	40.0	35.0	41.0	33.0	41.0
50-51	36.737207077774855	40.0	35.0	41.0	32.5	41.0
52-53	36.28406556278668	39.0	35.0	41.0	31.0	41.0
54-55	35.498554478383284	39.0	35.0	41.0	28.0	41.0
56-57	34.86931903046046	39.0	35.0	41.0	23.5	41.0
58-59	34.55435188616154	39.0	35.0	41.0	22.0	41.0
60-61	34.29990441887496	38.0	35.0	40.5	21.5	41.0
62-63	33.996461311389936	38.0	35.0	40.0	20.0	41.0
64-65	33.71845095950178	37.0	34.0	40.0	18.0	41.0
66-67	33.391825701381805	36.5	34.0	40.0	17.5	41.0
68-69	33.09241273251923	36.0	34.0	39.0	14.5	41.0
70-71	32.75240599263334	35.5	34.0	39.0	11.5	41.0
72-73	32.396786426386754	35.0	33.5	39.0	7.5	40.5
74-75	32.032179972846606	35.0	33.0	37.5	4.5	40.0
76-77	31.657162483190668	35.0	33.0	37.0	2.0	39.5
78-79	31.518121023200166	35.0	33.0	37.0	2.0	39.0
80-81	31.371090859974146	35.0	33.0	36.0	2.0	39.0
82-83	31.047044005838252	35.0	33.0	36.0	2.0	38.0
84-85	30.75456563895833	35.0	33.0	36.0	2.0	37.0
86-87	30.550006623081078	35.0	33.0	35.0	2.0	37.0
88-89	30.326927459492964	35.0	33.0	35.0	2.0	36.5
90-91	30.11332750303382	35.0	33.0	35.0	2.0	36.0
92-93	29.934498722229968	35.0	33.0	35.0	2.0	36.0
94-95	29.73851119101885	35.0	33.0	35.0	2.0	36.0
96-97	29.546375857490183	35.0	33.0	35.0	2.0	36.0
98-99	29.38561491642119	35.0	32.5	35.0	2.0	35.0
100	29.266797338937977	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	2.77392573635861
1101	2	2.2123996024396853
1101	3	2.0329678140955565
1101	4	0.9731996840920516
1101	5	1.5685368085758
1101	6	1.0324997292377631
1101	7	0.562708521525991
1101	8	0.44220238901683473
1101	9	0.4231541290869032
1101	10-11	0.3458138297869624
1101	12-13	0.2664991469872504
1101	14-15	0.4064620895613231
1101	16-17	0.3767600881030475
1101	18-19	0.3890176877231397
1101	20-21	0.42214394525215937
1101	22-23	0.3650388354601759
1101	24-25	0.4061660596357797
1101	26-27	0.40809856717189064
1101	28-29	0.4695113232556736
1101	30-31	0.48996066336967203
1101	32-33	0.4922128867522275
1101	34-35	0.520872855695238
1101	36-37	0.5109329361466521
1101	38-39	0.42397508844055665
1101	40-41	0.4568647924780507
1101	42-43	0.4307385328948925
1101	44-45	0.4130902334020021
1101	46-47	0.41559366262750785
1101	48-49	0.3659972878898188
1101	50-51	0.44435825933509676
1101	52-53	0.3405850976359801
1101	54-55	0.3587728504460159
1101	56-57	0.25312498866451705
1101	58-59	0.2508517085151354
1101	60-61	0.27690233440843315
1101	62-63	0.21380567967943875
1101	64-65	0.18755825719322416
1101	66-67	0.18853290025885627
1101	68-69	0.1965347022103714
1101	70-71	0.24518590712152388
1101	72-73	0.18352913178304675
1101	74-75	0.24123498134073174
1101	76-77	0.15511082707353907
1101	78-79	0.24510877539148268
1101	80-81	0.26030224340817654
1101	82-83	0.38783032344899127
1101	84-85	0.36748349700011573
1101	86-87	0.402652810042877
1101	88-89	0.33072869520034587
1101	90-91	0.24124681557166738
1101	92-93	0.3165050289102709
1101	94-95	0.34597625012243327
1101	96-97	0.3200538599724787
1101	98-99	0.32877872536095865
1101	100	0.30927958566882197
1102	1	2.7549944575187943
1102	2	2.272330569992601
1102	3	2.0921315510031917
1102	4	1.0587738253064387
1102	5	1.551811088953741
1102	6	1.0154421228508923
1102	7	0.44535473481244736
1102	8	0.39908274498255736
1102	9	0.3707920108711278
1102	10-11	0.30415975192860856
1102	12-13	0.2518322697441491
1102	14-15	0.40759027567427353
1102	16-17	0.3405970195466921
1102	18-19	0.3802393972375313
1102	20-21	0.38953526950323436
1102	22-23	0.3405277389978565
1102	24-25	0.37888199203158734
1102	26-27	0.3251948555334181
1102	28-29	0.3920633722558904
1102	30-31	0.38894623430645936
1102	32-33	0.3954301283841062
1102	34-35	0.4085239213950871
1102	36-37	0.4064211072445758
1102	38-39	0.3476047394330095
1102	40-41	0.3290604637590917
1102	42-43	0.32224474947732773
1102	44-45	0.3064598225032995
1102	46-47	0.3161466546439229
1102	48-49	0.3577834337918233
1102	50-51	0.3034114436169375
1102	52-53	0.2509786466728272
1102	54-55	0.2708425420888787
1102	56-57	0.19519609940940796
1102	58-59	0.1476674266723208
1102	60-61	0.1403524461054957
1102	62-63	0.12037863983471198
1102	64-65	0.12015892188230026
1102	66-67	0.15640946584883864
1102	68-69	0.10367644761430483
1102	70-71	0.040755333254978154
1102	72-73	0.1241360496765509
1102	74-75	0.1340092342521828
1102	76-77	0.045370522744821784
1102	78-79	0.14334153559070728
1102	80-81	0.2093058964514647
1102	82-83	0.3107302813337043
1102	84-85	0.2859972851785386
1102	86-87	0.29233021610747656
1102	88-89	0.29803693331651004
1102	90-91	0.24313994345564893
1102	92-93	0.3373479755154136
1102	94-95	0.29385789701322906
1102	96-97	0.24905275215734335
1102	98-99	0.26735083405501925
1102	100	0.2008102932056275
1103	1	2.626137395692364
1103	2	2.21481609700734
1103	3	2.0355605230836105
1103	4	1.0374130045553756
1103	5	1.623716053435281
1103	6	1.0318229311772456
1103	7	0.5813305696538578
1103	8	0.48042744962561557
1103	9	0.4462292390806226
1103	10-11	0.34258051964957303
1103	12-13	0.27112253075308246
1103	14-15	0.4109462976065785
1103	16-17	0.4223235814136359
1103	18-19	0.3964170098026898
1103	20-21	0.4624487849755994
1103	22-23	0.4013070180946343
1103	24-25	0.5077702281730936
1103	26-27	0.47124590263026533
1103	28-29	0.5434863052975132
1103	30-31	0.5277609013152613
1103	32-33	0.5491329505036902
1103	34-35	0.5733314396945204
1103	36-37	0.514149129915161
1103	38-39	0.5200823772077285
1103	40-41	0.545939498413226
1103	42-43	0.580040128926143
1103	44-45	0.5435573699737901
1103	46-47	0.47131950890568675
1103	48-49	0.5226361942380322
1103	50-51	0.5408802693018728
1103	52-53	0.6229942405829192
1103	54-55	0.5454428468543853
1103	56-57	0.5064391105106196
1103	58-59	0.5185219742958722
1103	60-61	0.5263526464314197
1103	62-63	0.5423078009867908
1103	64-65	0.47157577762062175
1103	66-67	0.5048086028717691
1103	68-69	0.4940587212460201
1103	70-71	0.4535103549081825
1103	72-73	0.48560217658864957
1103	74-75	0.4716552583453151
1103	76-77	0.4652507323442556
1103	78-79	0.39458321868860224
1103	80-81	0.5250680578106177
1103	82-83	0.5671270932254124
1103	84-85	0.5242054598267316
1103	86-87	0.5107348227033803
1103	88-89	0.49556690342518195
1103	90-91	0.5278570449871296
1103	92-93	0.5131030804501862
1103	94-95	0.5064899095459765
1103	96-97	0.4694834078758596
1103	98-99	0.47662991957843204
1103	100	0.5278870427937221
1104	1	1.757569349844207
1104	2	1.8238112538845321
1104	3	1.8596588312317266
1104	4	0.870507422844085
1104	5	1.5338180853025989
1104	6	1.0188576501197915
1104	7	0.5088714115434101
1104	8	0.38798786337461877
1104	9	0.34853251411236386
1104	10-11	0.332847523281238
1104	12-13	0.27371027320200625
1104	14-15	0.3640700464483686
1104	16-17	0.34552760592830367
1104	18-19	0.3809481049825081
1104	20-21	0.44382649843987565
1104	22-23	0.35770381044499544
1104	24-25	0.40557927480619327
1104	26-27	0.36034451854627747
1104	28-29	0.4631602184001977
1104	30-31	0.4783313477530271
1104	32-33	0.36245870130755975
1104	34-35	0.34358612171580916
1104	36-37	0.3913669516919498
1104	38-39	0.3692469235442246
1104	40-41	0.3376716815117007
1104	42-43	0.3422656912017459
1104	44-45	0.39207986617299184
1104	46-47	0.46818106618945876
1104	48-49	0.3955618930754312
1104	50-51	0.35926592862514184
1104	52-53	0.2649070262419073
1104	54-55	0.18626154487088797
1104	56-57	0.2612685063213789
1104	58-59	0.3463110051211373
1104	60-61	0.3085889506237578
1104	62-63	0.22565896034573996
1104	64-65	0.23041052750337343
1104	66-67	0.26213164041563886
1104	68-69	0.25115010216516964
1104	70-71	0.23025519462103006
1104	72-73	0.23458319248418036
1104	74-75	0.21733644403067132
1104	76-77	0.18297204878467355
1104	78-79	0.24204215891026948
1104	80-81	0.23807785626894784
1104	82-83	0.3300857967653954
1104	84-85	0.312981042011927
1104	86-87	0.23374891629423544
1104	88-89	0.290898159733743
1104	90-91	0.2855810523539475
1104	92-93	0.3170823831951992
1104	94-95	0.30358210210082603
1104	96-97	0.32676199610910217
1104	98-99	0.3631540161707072
1104	100	0.35611189202830573
1105	1	2.1652523827724686
1105	2	1.9731955728286756
1105	3	1.9039067763135833
1105	4	0.9073234881286467
1105	5	1.55185835686828
1105	6	1.014579483410543
1105	7	0.520487084973162
1105	8	0.3638484536861526
1105	9	0.31228221065684636
1105	10-11	0.24661106597663718
1105	12-13	0.22347349051683096
1105	14-15	0.30500314515180094
1105	16-17	0.294898793669077
1105	18-19	0.2426287115376482
1105	20-21	0.3131916790324425
1105	22-23	0.29666508380154255
1105	24-25	0.3623913983465812
1105	26-27	0.2884893503969792
1105	28-29	0.28674258067587743
1105	30-31	0.35690449673792557
1105	32-33	0.33433044035233905
1105	34-35	0.29893129203856716
1105	36-37	0.33451873124406717
1105	38-39	0.26976630116490696
1105	40-41	0.2671788856409876
1105	42-43	0.27316489821370027
1105	44-45	0.32737981367995417
1105	46-47	0.3453897377724289
1105	48-49	0.31793658183493534
1105	50-51	0.33462796217747837
1105	52-53	0.3020870792687802
1105	54-55	0.3205605101987885
1105	56-57	0.26187930984616514
1105	58-59	0.23864240618956245
1105	60-61	0.3551063378304562
1105	62-63	0.3642554754054004
1105	64-65	0.29329735384801126
1105	66-67	0.3570026781763076
1105	68-69	0.29133400732943926
1105	70-71	0.30934737964055614
1105	72-73	0.31856867714235904
1105	74-75	0.29728048905774784
1105	76-77	0.25272304685145386
1105	78-79	0.23690049443611016
1105	80-81	0.24793166061608218
1105	82-83	0.3336276469352697
1105	84-85	0.34384139559638527
1105	86-87	0.2291802822825595
1105	88-89	0.155535987958217
1105	90-91	0.15621621811374808
1105	92-93	0.20644933368016183
1105	94-95	0.1870639220967334
1105	96-97	0.26138770835574476
1105	98-99	0.23662668960427524
1105	100	0.2296358430890315
1106	1	1.6798914542826502
1106	2	1.1215095120656642
1106	3	1.5192884161347209
1106	4	0.7587943021106369
1106	5	1.4484899712405124
1106	6	0.9660174377751716
1106	7	0.48985730007132133
1106	8	0.3928247218760106
1106	9	0.31401227765463346
1106	10-11	0.28664602563708286
1106	12-13	0.21530274001457173
1106	14-15	0.3008394000186527
1106	16-17	0.3036912098566944
1106	18-19	0.301081522358281
1106	20-21	0.3636150570825478
1106	22-23	0.27772947480737287
1106	24-25	0.38628412187545536
1106	26-27	0.28096589262056426
1106	28-29	0.3145086774093855
1106	30-31	0.27674362305485545
1106	32-33	0.29777645733510383
1106	34-35	0.2636463946770178
1106	36-37	0.21835807519997985
1106	38-39	0.22797928665863054
1106	40-41	0.2480243652244596
1106	42-43	0.26370564454641254
1106	44-45	0.2828563554963637
1106	46-47	0.21312919256337182
1106	48-49	0.18259137567442707
1106	50-51	0.11790163311385271
1106	52-53	0.09206775642935128
1106	54-55	0.07807310129566503
1106	56-57	0.12395091327334029
1106	58-59	0.21364520047857383
1106	60-61	0.15538061078597565
1106	62-63	0.2160090520953446
1106	64-65	0.1793871438722121
1106	66-67	0.18363590193686719
1106	68-69	0.2195134247084809
1106	70-71	0.15135863456848142
1106	72-73	0.15798920878409461
1106	74-75	0.1834811344825269
1106	76-77	0.12518329770837155
1106	78-79	0.10640124202294388
1106	80-81	0.21832812798326628
1106	82-83	0.22687878236331116
1106	84-85	0.1913813982104955
1106	86-87	0.16402608944424557
1106	88-89	0.164710544801288
1106	90-91	0.20508807863586753
1106	92-93	0.21867590053828323
1106	94-95	0.2420926854594434
1106	96-97	0.23551409348837282
1106	98-99	0.19862326458975943
1106	100	0.14594393477998935
1107	1	1.639969344319148
1107	2	1.0301658671443228
1107	3	1.4232922649114244
1107	4	0.7317744933006409
1107	5	1.494617520723203
1107	6	1.0256254407527123
1107	7	0.5699024646891289
1107	8	0.42519191130043055
1107	9	0.4111369051547058
1107	10-11	0.2310281539004606
1107	12-13	0.1848268496899479
1107	14-15	0.29251125787941135
1107	16-17	0.3084031342303035
1107	18-19	0.2690902622477438
1107	20-21	0.2592207256206649
1107	22-23	0.25658155797988513
1107	24-25	0.3284234330388287
1107	26-27	0.26629983103325827
1107	28-29	0.28150259006850575
1107	30-31	0.16624481608712927
1107	32-33	0.17976796327961608
1107	34-35	0.14870020527570915
1107	36-37	0.2050782222283587
1107	38-39	0.1934404244864254
1107	40-41	0.17510592752886822
1107	42-43	0.15378041740811454
1107	44-45	0.18558939012503117
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2209	6	1.029598082125112
2209	7	0.5567787934303041
2209	8	0.46202697478761223
2209	9	0.4425538643723712
2209	10-11	0.31048536725550946
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2209	16-17	0.32904151478365407
2209	18-19	0.3222440594999938
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2210	2	1.6343062247017563
2210	3	1.7370939188733203
2210	4	0.9065607702967426
2210	5	1.5158012827656293
2210	6	0.9976688082694025
2210	7	0.5456100842261336
2210	8	0.4442870566228265
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2210	14-15	0.3101406792884944
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2210	18-19	0.25883483899764315
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2210	24-25	0.3683886643495029
2210	26-27	0.3388750338043991
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2210	30-31	0.29716413461342484
2210	32-33	0.33390143549054585
2210	34-35	0.3334501100823317
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2211	2	-0.10031257023322127
2211	3	-1.064550311777765
2211	4	-0.41057279034211547
2211	5	-1.4206456568315033
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2211	10-11	-0.03518495581044334
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2211	30-31	-0.013581223988168745
2211	32-33	-0.00594992357220292
2211	34-35	-0.06064532273177292
2211	36-37	-0.04287785096347818
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2211	48-49	-0.06454872441804582
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2211	66-67	-0.08062740500599119
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2211	84-85	-0.3281494453062308
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2211	88-89	-0.06891341154342712
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2212	2	0.7139212167578002
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2212	4	-0.1626491911681356
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2212	6	-0.1530701166176982
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2212	9	0.029145590071983918
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2212	56-57	0.08404857523337483
2212	58-59	0.09622683987679892
2212	60-61	0.0745602549584703
2212	62-63	-1.218401447223016
2212	64-65	-0.21172558085967097
2212	66-67	0.06702483800052761
2212	68-69	0.07007785578279879
2212	70-71	0.08858834395635284
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2212	74-75	0.08731722134609043
2212	76-77	0.1325110556123903
2212	78-79	0.10539940164677475
2212	80-81	0.10725261152512999
2212	82-83	0.1323666041186442
2212	84-85	0.1768363962888344
2212	86-87	0.16491959238219067
2212	88-89	0.17363903300775263
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2212	92-93	0.20367890711214898
2212	94-95	0.19764272997490195
2212	96-97	0.18896140520348936
2212	98-99	0.16906331354470083
2212	100	0.1963123388468624
2213	1	-2.899175057305392
2213	2	-2.845613719628922
2213	3	-2.707424772824595
2213	4	-1.5019684024576918
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2213	34-35	-0.4245589462175374
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2213	70-71	-0.422883136860861
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2213	84-85	-0.2773877562981468
2213	86-87	-0.30101791199576056
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2213	90-91	-0.3209799852590365
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2213	98-99	-0.33959077788111713
2213	100	-0.29286131986560093
2214	1	-2.6251979967494954
2214	2	-2.6805228572796516
2214	3	-2.7059030279002876
2214	4	-1.2460863086314404
2214	5	-2.1492459681913516
2214	6	-1.2153108216977841
2214	7	-0.6753607677151692
2214	8	-0.6920215584883636
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2214	16-17	-0.5210297540227344
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2214	22-23	-0.5384585170606186
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2214	82-83	-0.2638166360104677
2214	84-85	-0.3174847494915696
2214	86-87	-0.3925918787565017
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2214	90-91	-0.3740842005405973
2214	92-93	-0.41518229242548443
2214	94-95	-0.3905004910418093
2214	96-97	-0.3998582697052804
2214	98-99	-0.4848732529394546
2214	100	-0.5169637550276427
2215	1	-5.365375536344828
2215	2	-4.3281656179792805
2215	3	-3.48371557890642
2215	4	-1.7547837650284848
2215	5	-2.4219945666157017
2215	6	-4.891908260898475
2215	7	-2.072721901698081
2215	8	-1.0947392927376711
2215	9	-1.5188458012769246
2215	10-11	-0.776215496257791
2215	12-13	-0.5329361087823088
2215	14-15	-0.7128641241561766
2215	16-17	-0.6091960626142026
2215	18-19	-0.6619662816205576
2215	20-21	-0.6406150398620909
2215	22-23	-0.740554126780637
2215	24-25	-0.7841878201230301
2215	26-27	-0.7337980098006582
2215	28-29	-0.7296273131452011
2215	30-31	-0.8308290620180401
2215	32-33	-0.7726603242126018
2215	34-35	-0.677502624589529
2215	36-37	-0.6599838752540208
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2215	40-41	-0.7299012627557175
2215	42-43	-0.7422299086066246
2215	44-45	-0.7225365535850443
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2215	56-57	-0.9125952490405993
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2215	60-61	-0.8145156474943605
2215	62-63	-0.7730916858203472
2215	64-65	-0.8424166584241206
2215	66-67	-0.9013591022316589
2215	68-69	-0.921167427017977
2215	70-71	-0.8674183167218992
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2215	80-81	-0.7822022163789804
2215	82-83	-0.7975887070926149
2215	84-85	-0.7812629495070169
2215	86-87	-0.7986805946333995
2215	88-89	-0.8565416334414238
2215	90-91	-0.8222196167214904
2215	92-93	-0.8177921168259488
2215	94-95	-0.8411241867421388
2215	96-97	-0.8131476169287986
2215	98-99	-0.8094881429775214
2215	100	-0.8340495251182318
2216	1	-5.71584822302151
2216	2	-4.75242096666171
2216	3	-5.680748886949914
2216	4	-2.9545635120786784
2216	5	-4.202268867452478
2216	6	-1.9544731291392026
2216	7	-1.2438632287957603
2216	8	-0.7883645526802425
2216	9	-0.6345866106743827
2216	10-11	-1.0521778201310639
2216	12-13	-0.7980824784248526
2216	14-15	-1.6606662116851183
2216	16-17	-1.1650142877894751
2216	18-19	-1.762020618195308
2216	20-21	-1.199629295699168
2216	22-23	-0.8822167004083781
2216	24-25	-1.484937488873591
2216	26-27	-1.205748211358106
2216	28-29	-2.028434157694946
2216	30-31	-2.0178211766895657
2216	32-33	-1.255178401306864
2216	34-35	-1.2888209746321522
2216	36-37	-1.2260001441939679
2216	38-39	-1.0811130292759827
2216	40-41	-1.1620805716727887
2216	42-43	-1.5037646162914768
2216	44-45	-1.4682317196665267
2216	46-47	-1.8689113046685506
2216	48-49	-1.4814540528834357
2216	50-51	-1.3613148489493199
2216	52-53	-1.2098293762802044
2216	54-55	-1.1274898432653089
2216	56-57	-1.0610406752871029
2216	58-59	-1.0951569346681609
2216	60-61	-0.9859768791985246
2216	62-63	-1.009981751342579
2216	64-65	-1.0837618336902253
2216	66-67	-1.171043579351867
2216	68-69	-1.1181684059858839
2216	70-71	-1.0257175611706408
2216	72-73	-1.0595274521981963
2216	74-75	-1.010386717412814
2216	76-77	-0.9606116897191832
2216	78-79	-0.9349837498637115
2216	80-81	-0.83494119597054
2216	82-83	-0.8590292501657188
2216	84-85	-0.8113931536231114
2216	86-87	-0.8731844570187057
2216	88-89	-0.9107241507473631
2216	90-91	-0.9323255275640321
2216	92-93	-1.0566810485566265
2216	94-95	-1.1150903632130174
2216	96-97	-1.1187263647238446
2216	98-99	-1.111492624756277
2216	100	-1.1894425586868849
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	5307.0
3	3506.0
4	3735.0
5	4988.0
6	4310.0
7	3415.0
8	3472.0
9	3922.0
10	4612.0
11	10098.0
12	6905.0
13	9440.0
14	9111.0
15	9358.0
16	11117.0
17	14277.0
18	19251.0
19	31455.0
20	63974.0
21	86916.0
22	40790.0
23	26844.0
24	27798.0
25	30368.0
26	30843.0
27	33085.0
28	38065.0
29	44394.0
30	52375.0
31	64266.0
32	82397.0
33	111174.0
34	159820.0
35	314778.0
36	465340.0
37	789132.0
38	1015451.0
39	384467.0
40	3251.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	14.225373757106247	42.21434891091847	25.49960685939591	18.060670472579375
2	17.504668506796538	39.702837014712166	25.910393020007245	16.88210145848405
3	18.465721641221858	25.4560917061877	20.47553473613421	35.60265191645623
4	32.972232515128084	26.13087556137757	22.604488734176364	18.292403189317977
5	17.664987411175538	39.4386957426139	23.576975734671173	19.319341111539394
6	35.947176392903536	23.531148486992542	23.698974627759235	16.822700492344687
7	18.69587681516534	23.932062347920763	39.37768387002657	17.99437696688733
8	34.60359803539285	23.883526222803432	23.619323690226693	17.89355205157703
9	34.35863101783958	25.17066549166001	23.678496508406095	16.79220698209432
10-11	16.84440878998421	42.36658219442433	23.310909295599913	17.47809971999154
12-13	16.864054364436466	25.01949024891104	31.69247431598981	26.423981070662684
14-15	17.22808772885976	33.46240264505728	23.018636331215685	26.29087329486727
16-17	26.050312092736128	24.71234930926059	31.60914398415032	17.62819461385296
18-19	17.426544066353085	24.970506786235024	31.640359490402993	25.9625896570089
20-21	26.214865603376314	33.67840938376892	23.02776555190754	17.078959460947228
22-23	25.8063081120025	25.074686807480383	31.741551462633733	17.377453617883383
24-25	17.25023093801467	33.5756038994912	23.029857048312703	26.14430811418142
26-27	17.604964268712404	28.702061242168153	36.41156697375025	17.281407515369192
28-29	25.419472959373046	24.849596876759467	32.4642601918425	17.266669972024985
30-31	23.07403433609729	26.16817017883829	32.837232722409574	17.92056276265484
32-33	19.51562202353844	27.481085159559154	33.741376377334774	19.261916439567635
34-35	19.50969561786653	27.215254521618647	34.00253496449186	19.27251489602296
36-37	19.54571991906523	27.220878307796198	34.325623520095874	18.907778253042697
38-39	20.09719127577624	27.121692410229553	33.889861900798685	18.891254413195526
40-41	19.117352661910573	28.27101413022583	33.6938864042228	18.917746803640796
42-43	19.092845316874467	27.247161724214248	34.28341637203493	19.37657658687636
44-45	19.13871281604575	27.79685342883309	33.59509611071278	19.46933764440838
46-47	19.688363355890793	27.387598231439554	33.88506096776755	19.038977444902105
48-49	19.155851377730766	27.65179046408826	33.752275755663966	19.44008240251701
50-51	19.699960319970415	28.141939430106294	33.162438707527116	18.99566154239618
52-53	19.78185406030421	27.91285097668616	33.019777281134886	19.28551768187475
54-55	19.850644522182755	28.794551657704893	31.39699574228656	19.957808077825792
56-57	20.116231417052063	29.416037097427967	30.47597248658787	19.9917589989321
58-59	21.26722511392284	29.498399016247422	29.442096180304272	19.79227968952546
60-61	20.730164115124776	30.0759466614971	29.09400224553084	20.099886977847287
62-63	20.00837776851885	30.183837467811387	29.182469214931515	20.625315548738246
64-65	20.466194075997592	30.07264080789443	29.538292072366918	19.922873043741067
66-67	20.378457765158295	29.48573696726755	30.103782663851142	20.032022603723014
68-69	20.664852503893837	29.49882355309707	29.942944220397788	19.893379722611307
70-71	19.56330683846081	30.346446555418026	30.037480551371722	20.05276605474944
72-73	19.515720337292823	29.545257131907533	30.355496554040627	20.58352597675902
74-75	19.717321530666805	29.865869262636956	29.717039453630452	20.699769753065787
76-77	20.461631450874723	29.457197882206103	29.90374838982101	20.177422277098167
78-79	20.15974012464582	29.822568555882512	29.326950621768788	20.690740697702886
80-81	20.55765912013822	30.381886343388647	28.900736343568838	20.159718192904293
82-83	20.56341686187035	30.23687889501252	29.039249920779408	20.160454322337724
84-85	20.30042308858019	30.587215435432547	28.5064179059408	20.605943570046467
86-87	20.287961677351543	30.851680548991723	28.606657128587482	20.25370064506925
88-89	21.42567515090512	30.27649732362818	28.145477817025018	20.152349708441676
90-91	20.798884669159236	30.910453790977865	28.04010975853594	20.250551781326955
92-93	20.26873968682496	31.084925509019424	27.931601171454894	20.71473363270072
94-95	20.67714405795812	31.12836796814282	27.906027080692052	20.28846089320701
96-97	20.559536988386434	30.58125246371351	28.22339475239586	20.635815795504197
98-99	21.15075941437854	30.70725127745419	27.481024736901162	20.660964571266106
100	20.49836457348807	31.44127040617379	27.36929324871183	20.691071771626312
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	86871.0
1	47908.5
2	6863.0
3	4194.5
4	3542.0
5	3233.5
6	3229.0
7	3757.0
8	3562.0
9	3157.5
10	3232.5
11	4856.5
12	42811.0
13	47773.0
14	14327.0
15	12652.0
16	13821.5
17	15332.5
18	16873.5
19	18333.5
20	20163.5
21	22981.0
22	26278.0
23	30188.5
24	34162.5
25	38480.0
26	45449.5
27	52686.5
28	57568.5
29	61975.5
30	66299.5
31	71087.5
32	76221.5
33	82184.0
34	88841.5
35	94408.0
36	100006.0
37	104131.0
38	106190.0
39	108132.0
40	109610.0
41	110725.5
42	111873.0
43	112084.0
44	112722.5
45	113438.0
46	113778.0
47	116653.0
48	118267.0
49	126923.5
50	161054.5
51	159182.0
52	118505.0
53	103309.5
54	100502.5
55	98074.0
56	90525.5
57	80169.5
58	74394.0
59	64159.5
60	50913.0
61	41527.5
62	33428.0
63	25792.0
64	19199.5
65	14627.0
66	10900.5
67	7788.5
68	5836.5
69	4382.5
70	3340.0
71	2629.0
72	2173.0
73	1675.0
74	1139.0
75	721.5
76	490.5
77	359.5
78	247.0
79	160.5
80	83.0
81	45.5
82	28.5
83	16.5
84	11.5
85	5.0
86	2.0
87	1.5
88	1.0
89	0.0
90	0.0
91	0.0
92	0.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05827814306203056
2	0.06744856301507503
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	7.455625978084933E-5
18-19	0.0
20-21	1.9881669274893156E-4
22-23	9.940834637446578E-5
24-25	0.0
26-27	0.002298818009909521
28-29	9.319532472606167E-4
30-31	0.005666275743344549
32-33	0.0012053261997903974
34-35	0.0020378711006765484
36-37	0.007952667709957261
38-39	0.01251302559988588
40-41	0.0031686410406860962
42-43	0.006995862376103029
44-45	0.0015408293688042194
46-47	0.0020502971439733566
48-49	0.0035662744261839596
50-51	0.009257402256122125
52-53	0.003094084780905247
54-55	0.0012674564162744387
56-57	5.964500782467947E-4
58-59	7.704146844021097E-4
60-61	0.006449116471043467
62-63	0.0015532554121010277
64-65	0.007728998930614714
66-67	0.00991598255085296
68-69	0.011518942136141222
70-71	0.009617757511729564
72-73	0.004510653716741385
74-75	0.0020751492305669727
76-77	0.004622488106412659
78-79	0.01609172606936665
80-81	0.00858639591809448
82-83	0.005765684089719014
84-85	0.004510653716741385
86-87	0.0070952707224774935
88-89	0.004212428677617987
90-91	0.0061508914319200695
92-93	0.010785805581629537
94-95	0.008698230307765755
96-97	0.010164503416789124
98-99	0.00202544505737974
100	0.0021372794470510144
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4023807.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.057170961668504
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.99804646666362	45.64462213847928
2	13.474936949507363	15.376835624514909
3	3.3921120977425536	5.806329596461225
4	1.162795843534279	2.6538336495221153
5	0.5296792101087178	1.511099862300732
6	0.32782355762447907	1.122281086358537
7	0.21981530041500252	0.8779427423038524
8	0.14250720113102647	0.6504846190561485
9	0.10584271446585573	0.5435177268892856
>10	0.5370104141029483	6.446881396201566
>50	0.08615122788888423	3.09959018699597
>100	0.01841040429794745	1.8556005510451465
>500	0.002105345967183083	0.8655012331712196
>1k	0.002456236933453055	2.6129958506192588
>5k	1.3158412143498505E-4	0.4090509350391413
>10k+	1.7544549524664674E-4	10.523432801041533
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	285718	7.100688477354902	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	96026	2.3864464672386125	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	27634	0.6867625609279968	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	11121	0.2763800550076085	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA	5597	0.13909712866447121	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5488	0.13638825122576703	No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5260	0.13072197548242248	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4577	0.11374800033898247	No Hit
CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4192	0.10417994700044012	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.005790536176312631	0.0	0.0	0.0	0.0
2	0.0058153882629062474	0.0	0.0	0.0	0.0
3	0.0058153882629062474	0.0	0.0	0.0	0.0
4	0.0058153882629062474	0.0	0.0	0.0	0.0
5	0.0058153882629062474	0.0	0.0	0.0	0.0
6	0.0058153882629062474	0.0	0.0	0.0	0.0
7	0.0058153882629062474	0.0	0.0	0.0	0.0
8	0.0058153882629062474	2.4852086593616445E-5	0.0	0.0	0.0
9	0.0058153882629062474	2.4852086593616445E-5	0.0	0.0	0.0
10-11	0.005827814306203056	2.4852086593616445E-5	0.0	0.0	2.4852086593616445E-5
12-13	0.005840240349499864	2.4852086593616445E-5	0.0	0.0	2.4852086593616445E-5
14-15	0.005840240349499864	2.4852086593616445E-5	0.0	0.0	2.4852086593616445E-5
16-17	0.0058650924360934805	2.4852086593616445E-5	0.0	0.0	2.4852086593616445E-5
18-19	0.005877518479390289	2.4852086593616445E-5	0.0	0.0	2.4852086593616445E-5
20-21	0.005952074739171138	2.4852086593616445E-5	0.0	0.0	2.4852086593616445E-5
22-23	0.0060514830855456034	2.4852086593616445E-5	0.0	0.0	2.4852086593616445E-5
24-25	0.006138465388623261	2.4852086593616445E-5	0.0	0.0	2.4852086593616445E-5
26-27	0.0062502997782945355	2.4852086593616445E-5	0.0	0.0	2.4852086593616445E-5
28-29	0.006374560211262618	2.4852086593616445E-5	0.0	0.0	2.4852086593616445E-5
30-31	0.006560950860714741	2.4852086593616445E-5	0.0	0.0	3.727812989042467E-5
32-33	0.006747341510166864	2.4852086593616445E-5	0.0	0.0	8.698230307765755E-5
34-35	0.006859175899838138	2.4852086593616445E-5	0.0	0.0	1.11834389671274E-4
36-37	0.007095270722477494	2.4852086593616445E-5	0.0	0.0	1.739646061553151E-4
38-39	0.0075798864110530155	2.4852086593616445E-5	0.0	0.0	0.0024355044861744113
40-41	0.008126632316112577	2.4852086593616445E-5	0.0	0.0	0.002460356572768028
42-43	0.00900888139018596	2.4852086593616445E-5	0.0	0.0	0.002460356572768028
44-45	0.010114799243601893	2.4852086593616445E-5	0.0	0.0	0.002460356572768028
46-47	0.011158586880533783	2.4852086593616445E-5	0.0	0.0	0.002460356572768028
48-49	0.012252078690652906	2.4852086593616445E-5	0.0	0.0	0.002460356572768028
50-51	0.014376932094407113	2.4852086593616445E-5	0.0	0.0	0.002460356572768028
52-53	0.01657634175794217	2.4852086593616445E-5	0.0	0.0	0.002460356572768028
54-55	0.019310071283239978	2.4852086593616445E-5	0.0	2.4852086593616445E-5	0.002460356572768028
56-57	0.02250356441051969	2.4852086593616445E-5	0.0	2.4852086593616445E-5	0.002460356572768028
58-59	0.02662901078506002	2.4852086593616445E-5	0.0	4.970417318723289E-5	0.002460356572768028
60-61	0.03241954696137265	2.4852086593616445E-5	0.0	4.970417318723289E-5	0.002460356572768028
62-63	0.04002428545901929	2.4852086593616445E-5	0.0	4.970417318723289E-5	0.002460356572768028
64-65	0.04986571175009139	2.4852086593616445E-5	0.0	4.970417318723289E-5	0.002460356572768028
66-67	0.06324856038075385	2.4852086593616445E-5	0.0	4.970417318723289E-5	0.002460356572768028
68-69	0.07960123335935347	2.4852086593616445E-5	0.0	4.970417318723289E-5	0.002460356572768028
70-71	0.10204266755338912	2.4852086593616445E-5	0.0	4.970417318723289E-5	0.002460356572768028
72-73	0.13215097046155544	2.4852086593616445E-5	0.0	4.970417318723289E-5	0.002460356572768028
74-75	0.16976460352099393	2.4852086593616445E-5	0.0	9.940834637446578E-5	0.002460356572768028
76-77	0.21844984115788854	2.4852086593616445E-5	0.0	9.940834637446578E-5	0.002460356572768028
78-79	0.2793126012256552	2.4852086593616445E-5	0.0	9.940834637446578E-5	0.002460356572768028
80-81	0.35682625931114487	2.4852086593616445E-5	0.0	9.940834637446578E-5	0.002460356572768028
82-83	0.4547310544466969	2.4852086593616445E-5	0.0	9.940834637446578E-5	0.002460356572768028
84-85	0.5806565772165514	2.4852086593616445E-5	0.0	1.242604329680822E-4	0.002460356572768028
86-87	0.7298312269947341	2.4852086593616445E-5	0.0	1.242604329680822E-4	0.002460356572768028
88	0.8619697714129927	2.4852086593616445E-5	0.0	1.242604329680822E-4	0.002460356572768028
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	2825	0.0	84.4312	1
AACGAGT	86935	0.0	68.439415	1
GTGGAAT	95565	0.0	68.192566	6
ACGAGTG	91295	0.0	67.98591	2
GAATCAC	97165	0.0	67.5291	9
TGGAATC	97380	0.0	67.28349	7
CGAGTGG	93995	0.0	67.04187	3
AGTGGAA	95030	0.0	67.02376	5
GGAATCA	98000	0.0	66.95373	8
GAGTGGA	96815	0.0	65.80745	4
ATCGAGT	1275	0.0	64.20258	1
AACGTGT	1760	0.0	61.211952	1
TCGAGTG	1485	0.0	60.478207	2
CGTGTGG	2380	0.0	56.66658	3
ACGTGTG	2415	0.0	50.038925	2
GAGTACT	42885	0.0	41.508537	20-21
AGTACTT	43745	0.0	41.40688	20-21
CGATTGG	835	0.0	41.08265	3
GTACTTT	43340	0.0	40.2985	22-23
TACTTTT	47180	0.0	37.810455	22-23
>>END_MODULE