FastQCFastQC Report
Thu 26 May 2016
SRR2132672_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132672_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3237188
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1989566.1459513627259215No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA537061.6590324689205571No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG156680.4840003113813594No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT77360.2389728369189556No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA46540.1437667506490201No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT45240.13975091962530442No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT35030.10821120058519926No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT20750.084.343141
AACGAGT576450.071.852741
ACGAGTG610200.070.946232
GTGGAAT648100.070.1946956
CGAGTGG626350.069.9840853
GAATCAC654500.069.8744359
TGGAATC656600.069.693897
GGAATCA657100.069.57658
AGTGGAA645450.068.757585
GAGTGGA649650.068.305844
CGTGTGG16850.058.0022243
AACGTGT13850.056.387551
ATCGAGT7750.054.6343421
ACGTGTG17300.053.2655222
TCGAGTG9550.053.168772
GAGTACT301950.041.36388420-21
AGTACTT308350.041.16062520-21
GTACTTT302350.040.33968722-23
TACTTTT329850.037.95952222-23
CATGGGG137100.037.77541424-25
Produced by FastQC (version 0.11.5)