##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132672_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3237188
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.200098048058994	31.0	28.0	34.0	16.0	34.0
2	30.320222674741164	33.0	30.0	34.0	22.0	34.0
3	30.789040982482327	33.0	31.0	34.0	25.0	34.0
4	35.241835506618706	37.0	35.0	37.0	32.0	37.0
5	34.71302469921426	37.0	35.0	37.0	32.0	37.0
6	35.26887934837272	37.0	35.0	37.0	32.0	37.0
7	35.688276059345334	37.0	35.0	37.0	35.0	37.0
8	35.88912290543521	37.0	35.0	37.0	35.0	37.0
9	37.81531378467979	39.0	39.0	39.0	35.0	39.0
10-11	37.91394197680209	39.0	39.0	39.0	35.0	39.0
12-13	37.92497439135447	39.0	39.0	39.0	35.0	39.0
14-15	39.36630757929413	41.0	39.0	41.0	37.0	41.0
16-17	39.32836260977119	41.0	39.0	41.0	36.5	41.0
18-19	39.28194068432232	41.0	39.0	41.0	36.0	41.0
20-21	39.16611608593631	41.0	39.0	41.0	36.0	41.0
22-23	39.09255795461988	40.5	39.0	41.0	36.0	41.0
24-25	39.03274215152163	41.0	39.0	41.0	35.0	41.0
26-27	38.988817918514464	41.0	39.0	41.0	35.0	41.0
28-29	38.8423744620331	41.0	39.0	41.0	35.0	41.0
30-31	38.738153144025006	41.0	39.0	41.0	35.0	41.0
32-33	38.61425332727046	40.5	38.5	41.0	35.0	41.0
34-35	38.54956941024123	40.0	38.0	41.0	35.0	41.0
36-37	38.42176512454637	40.0	38.0	41.0	35.0	41.0
38-39	38.035174972846804	40.0	37.0	41.0	34.5	41.0
40-41	37.852284142904274	40.0	37.0	41.0	34.0	41.0
42-43	37.72803618449099	40.0	36.5	41.0	33.0	41.0
44-45	37.60330493625949	40.0	36.0	41.0	33.0	41.0
46-47	37.48014047994741	40.0	36.0	41.0	33.0	41.0
48-49	37.33604628461492	40.0	35.0	41.0	33.0	41.0
50-51	37.09921805591767	40.0	35.0	41.0	33.0	41.0
52-53	36.674756146383835	40.0	35.0	41.0	31.5	41.0
54-55	35.95277521725646	39.5	35.0	41.0	29.0	41.0
56-57	35.359615042438065	39.0	35.0	41.0	26.5	41.0
58-59	35.04276473902659	39.0	35.0	41.0	25.0	41.0
60-61	34.79238879546075	39.0	35.0	41.0	24.5	41.0
62-63	34.49202764868768	38.0	35.0	40.5	23.0	41.0
64-65	34.22426794489539	38.0	35.0	40.0	22.5	41.0
66-67	33.91204774019921	37.0	34.0	40.0	22.0	41.0
68-69	33.60937764504255	37.0	34.0	40.0	21.5	41.0
70-71	33.26724490514607	36.0	34.0	39.0	20.0	41.0
72-73	32.914575088008476	36.0	34.0	39.0	18.5	41.0
74-75	32.55468032749411	35.0	34.0	38.5	17.5	40.0
76-77	32.176993427629164	35.0	33.0	37.0	14.0	39.5
78-79	32.04877195887295	35.0	33.5	37.0	12.5	39.0
80-81	31.897977812842505	35.0	34.0	37.0	8.5	39.0
82-83	31.577398346960386	35.0	34.0	36.0	4.5	39.0
84-85	31.29105553956088	35.0	33.0	36.0	2.0	37.0
86-87	31.08383927655731	35.0	33.0	36.0	2.0	37.0
88-89	30.86490327407614	35.0	33.0	35.0	2.0	36.5
90-91	30.66102663793391	35.0	33.0	35.0	2.0	36.0
92-93	30.492495184091872	35.0	33.0	35.0	2.0	36.0
94-95	30.319014372968144	35.0	33.0	35.0	2.0	36.0
96-97	30.152453610973474	35.0	33.0	35.0	2.0	36.0
98-99	30.009786425749756	35.0	33.0	35.0	2.0	35.5
100	29.904105970984695	35.0	33.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	2.8114355658571952
1101	2	2.267545116222326
1101	3	2.012302888181864
1101	4	1.028369880954564
1101	5	1.6221278162060315
1101	6	1.0766241242956767
1101	7	0.5790268222800989
1101	8	0.4521740323625565
1101	9	0.4222954088202897
1101	10-11	0.356674626645912
1101	12-13	0.29300816900345694
1101	14-15	0.42716753013543496
1101	16-17	0.36315122100236863
1101	18-19	0.3767307756136873
1101	20-21	0.3835286218268408
1101	22-23	0.36764155279743704
1101	24-25	0.41571560042147837
1101	26-27	0.3728358874269304
1101	28-29	0.3656237339957684
1101	30-31	0.38550059094310996
1101	32-33	0.3540242870835968
1101	34-35	0.32141731159430265
1101	36-37	0.35297670528651537
1101	38-39	0.3487363870495699
1101	40-41	0.3492968820769633
1101	42-43	0.32833627647514874
1101	44-45	0.40565794481348405
1101	46-47	0.3771174870460712
1101	48-49	0.33371684348917796
1101	50-51	0.3979784419459165
1101	52-53	0.304594340248066
1101	54-55	0.19064452611684857
1101	56-57	0.14031052361984564
1101	58-59	0.1021437798029794
1101	60-61	0.1343804795840029
1101	62-63	0.08720201938703553
1101	64-65	0.04723959017362489
1101	66-67	0.02721804191130417
1101	68-69	0.0458805509821687
1101	70-71	0.020478673471920672
1101	72-73	0.06660195399555846
1101	74-75	0.14187998118386247
1101	76-77	0.018736454984669138
1101	78-79	0.13457880521275456
1101	80-81	0.20122489569095237
1101	82-83	0.3218253689560946
1101	84-85	0.3650908018542971
1101	86-87	0.3306677177360626
1101	88-89	0.327401601822217
1101	90-91	0.24434113761289566
1101	92-93	0.35196271704572624
1101	94-95	0.34435629846942817
1101	96-97	0.3983701281254284
1101	98-99	0.3687133929241
1101	100	0.3731279746459215
1102	1	2.7818494119174417
1102	2	2.3030081471275743
1102	3	2.0471300629634186
1102	4	1.029916229392299
1102	5	1.5555556921211888
1102	6	0.9924782264049554
1102	7	0.4529888883023432
1102	8	0.4022069913016111
1102	9	0.3486358363665687
1102	10-11	0.2766890105378934
1102	12-13	0.2250785710626957
1102	14-15	0.3212255271371589
1102	16-17	0.28746039220686015
1102	18-19	0.3465602571315429
1102	20-21	0.3544066060776885
1102	22-23	0.2843143494142595
1102	24-25	0.3047386078119487
1102	26-27	0.2538907240571291
1102	28-29	0.3647116623181006
1102	30-31	0.32915175252828277
1102	32-33	0.2873036162772422
1102	34-35	0.33285889240488586
1102	36-37	0.28409909973677827
1102	38-39	0.32924760239194484
1102	40-41	0.2695303648108833
1102	42-43	0.30434639201793345
1102	44-45	0.24801703117802276
1102	46-47	0.19472803189882626
1102	48-49	0.21711136670612774
1102	50-51	0.25105991452205956
1102	52-53	0.3147195322921874
1102	54-55	0.3331209094381862
1102	56-57	0.31194864476733386
1102	58-59	0.2985687582565646
1102	60-61	0.25831995854286305
1102	62-63	0.23034664660400495
1102	64-65	0.2013945670253463
1102	66-67	0.13974454168582184
1102	68-69	0.1491140318987405
1102	70-71	0.15170284817221358
1102	72-73	0.1434782358222293
1102	74-75	0.22712998406132812
1102	76-77	0.16251496952956046
1102	78-79	0.21506285117370538
1102	80-81	0.29341442763420034
1102	82-83	0.30803114212296023
1102	84-85	0.20164455858919794
1102	86-87	0.26679723576534187
1102	88-89	0.25740554720237085
1102	90-91	0.23583386297748277
1102	92-93	0.23680547343940006
1102	94-95	0.22883424350013115
1102	96-97	0.16583953412114028
1102	98-99	0.15861186104523028
1102	100	0.1623509078365899
1103	1	2.6784194790463722
1103	2	2.196907160762148
1103	3	1.9951078687770334
1103	4	0.9715365841028358
1103	5	1.5764408065509485
1103	6	0.9936941406012068
1103	7	0.4751483606353304
1103	8	0.3810766983938265
1103	9	0.2980216575710912
1103	10-11	0.1969930083725373
1103	12-13	0.1476085446347355
1103	14-15	0.286575787735714
1103	16-17	0.3321875204675706
1103	18-19	0.3619493572208796
1103	20-21	0.3587019250637766
1103	22-23	0.3241343863989812
1103	24-25	0.4089831470147587
1103	26-27	0.37243887840151046
1103	28-29	0.37179337098066867
1103	30-31	0.3346048774051269
1103	32-33	0.351650263428013
1103	34-35	0.35621264553901
1103	36-37	0.3421520639898574
1103	38-39	0.3946534054747275
1103	40-41	0.34149615956228274
1103	42-43	0.3850310738804126
1103	44-45	0.26438403619210504
1103	46-47	0.2654020697955062
1103	48-49	0.2586517197799054
1103	50-51	0.2791362461958897
1103	52-53	0.3403738041414641
1103	54-55	0.34150154416199285
1103	56-57	0.2939093280002609
1103	58-59	0.25114730802418705
1103	60-61	0.3024317927981812
1103	62-63	0.24688097502878747
1103	64-65	0.2595183009895905
1103	66-67	0.28027461520749597
1103	68-69	0.34955745780343506
1103	70-71	0.3999202731844065
1103	72-73	0.44105510271295145
1103	74-75	0.34127783875803885
1103	76-77	0.38702026493189834
1103	78-79	0.4007780855950216
1103	80-81	0.404921582670017
1103	82-83	0.4848093107738869
1103	84-85	0.460891116070389
1103	86-87	0.5100399441792085
1103	88-89	0.4857293464692809
1103	90-91	0.4673100587801713
1103	92-93	0.47635134487364184
1103	94-95	0.44005453803963945
1103	96-97	0.4187622225719103
1103	98-99	0.40245598622846046
1103	100	0.4703297064385694
1104	1	1.542420024967317
1104	2	1.707286352162459
1104	3	1.7889968355742951
1104	4	0.8394637937725591
1104	5	1.5087844840305138
1104	6	0.9835388518859176
1104	7	0.46805808173404984
1104	8	0.36841505461345037
1104	9	0.3012160460784088
1104	10-11	0.22249579356521565
1104	12-13	0.18105701820287834
1104	14-15	0.25297238624334284
1104	16-17	0.22912845309448926
1104	18-19	0.27475579086006263
1104	20-21	0.34110250468032177
1104	22-23	0.26133756025015487
1104	24-25	0.33607674323472025
1104	26-27	0.2865058359974526
1104	28-29	0.3839621921358898
1104	30-31	0.3942458429603519
1104	32-33	0.40994924134449207
1104	34-35	0.3325655671058101
1104	36-37	0.3924263794704572
1104	38-39	0.37690575057578
1104	40-41	0.280553774955429
1104	42-43	0.3082666954180979
1104	44-45	0.35409881372034135
1104	46-47	0.3932362182282034
1104	48-49	0.31855491186653495
1104	50-51	0.2893898953688705
1104	52-53	0.26243073272556217
1104	54-55	0.2012589587039031
1104	56-57	0.10348874684586207
1104	58-59	0.10230544731533087
1104	60-61	0.13191545018773354
1104	62-63	0.08706996424081126
1104	64-65	0.09534167760533308
1104	66-67	0.10175356794697876
1104	68-69	0.054880709448347886
1104	70-71	0.036860314445192444
1104	72-73	-0.022179921315569118
1104	74-75	-0.011672542349963066
1104	76-77	0.10199602418076381
1104	78-79	-0.025712532449858827
1104	80-81	0.0394777500829413
1104	82-83	0.09337476798512867
1104	84-85	0.07785125061769804
1104	86-87	0.01644769945642821
1104	88-89	0.021173721059209782
1104	90-91	-0.0020945739402478125
1104	92-93	0.003716489421069724
1104	94-95	0.07049193271028287
1104	96-97	0.15253066556586248
1104	98-99	0.11238875175394725
1104	100	0.06052143015293865
1105	1	2.347355681248896
1105	2	2.069133022968547
1105	3	2.0029686144315733
1105	4	1.0201984200676506
1105	5	1.590557923527939
1105	6	1.0600603951611802
1105	7	0.5338399461800378
1105	8	0.4278812513335595
1105	9	0.3685486732744323
1105	10-11	0.2994350840189668
1105	12-13	0.23369240176936046
1105	14-15	0.3102532097455395
1105	16-17	0.2719494411997374
1105	18-19	0.25877715151359837
1105	20-21	0.34301166255070825
1105	22-23	0.3138435867668221
1105	24-25	0.36625117741176183
1105	26-27	0.28242043599502153
1105	28-29	0.2981261803506996
1105	30-31	0.3750964340459788
1105	32-33	0.40166506369008914
1105	34-35	0.35768214063540427
1105	36-37	0.3106384824204014
1105	38-39	0.33367462453026775
1105	40-41	0.21973617752674102
1105	42-43	0.2713679774225568
1105	44-45	0.28267698190276747
1105	46-47	0.3699877126825939
1105	48-49	0.25617970337341234
1105	50-51	0.2753128046545754
1105	52-53	0.3218649706451373
1105	54-55	0.2545654770817336
1105	56-57	0.22251321206860553
1105	58-59	0.19849839324712804
1105	60-61	0.269946270646237
1105	62-63	0.3120272003219924
1105	64-65	0.2259382723190413
1105	66-67	0.23025001291087932
1105	68-69	0.16654780223235122
1105	70-71	0.1546787154094318
1105	72-73	0.23537224762169728
1105	74-75	0.3168745801405066
1105	76-77	0.252027374242239
1105	78-79	0.15753999077354308
1105	80-81	0.2926812393752236
1105	82-83	0.3109700660846464
1105	84-85	0.31782531833383487
1105	86-87	0.24185311966899903
1105	88-89	0.18941169541912117
1105	90-91	0.19297205254963856
1105	92-93	0.21048079783262352
1105	94-95	0.24664427574691672
1105	96-97	0.21018586358867708
1105	98-99	0.2028207434263969
1105	100	0.17023142686574744
1106	1	1.6813233695314729
1106	2	1.2020046005738934
1106	3	1.613838761787072
1106	4	0.842170835257626
1106	5	1.5241279594830033
1106	6	1.0005349436104254
1106	7	0.5832142429376148
1106	8	0.44517915087932636
1106	9	0.4259751927906379
1106	10-11	0.32926945431451315
1106	12-13	0.2774063154535895
1106	14-15	0.3756427943952758
1106	16-17	0.3451057966472604
1106	18-19	0.3303028077851664
1106	20-21	0.4072810686242363
1106	22-23	0.3572756598400417
1106	24-25	0.40413885775402747
1106	26-27	0.3580730496181843
1106	28-29	0.40546314615828294
1106	30-31	0.3446265050179349
1106	32-33	0.31726816842575545
1106	34-35	0.3363464413631334
1106	36-37	0.27297144390120565
1106	38-39	0.2936261532262847
1106	40-41	0.3573404872882975
1106	42-43	0.3548185825449863
1106	44-45	0.3681573209378399
1106	46-47	0.4049339680312798
1106	48-49	0.26759766531086626
1106	50-51	0.255988899041121
1106	52-53	0.32695441096513633
1106	54-55	0.32162338884240427
1106	56-57	0.3302646676367331
1106	58-59	0.46127247528083615
1106	60-61	0.40621457514158266
1106	62-63	0.45397961407053344
1106	64-65	0.5255198139569046
1106	66-67	0.43981435054427465
1106	68-69	0.4296016279642956
1106	70-71	0.4446701040875922
1106	72-73	0.3977631926241756
1106	74-75	0.38632332196177543
1106	76-77	0.3747131591617503
1106	78-79	0.4056653119118465
1106	80-81	0.4032380147644865
1106	82-83	0.403394914283691
1106	84-85	0.33084854467180236
1106	86-87	0.3055361279653219
1106	88-89	0.2670809954975937
1106	90-91	0.24881707267689634
1106	92-93	0.30991686808759056
1106	94-95	0.26590488565963
1106	96-97	0.32131750373483214
1106	98-99	0.3602291129974873
1106	100	0.4265827427018216
1107	1	1.5424066639618594
1107	2	1.0659925474028462
1107	3	1.4153981979905552
1107	4	0.7331189413772918
1107	5	1.459370954035684
1107	6	0.9732543937356368
1107	7	0.5250282369983097
1107	8	0.4431060442482746
1107	9	0.4477770561016641
1107	10-11	0.2606342757768729
1107	12-13	0.1543785282184018
1107	14-15	0.2463221997127789
1107	16-17	0.19557388963917788
1107	18-19	0.20313501963781988
1107	20-21	0.16425916645420102
1107	22-23	0.2153781627049156
1107	24-25	0.2549707520192257
1107	26-27	0.23423379311222448
1107	28-29	0.2165341825615954
1107	30-31	0.15468739059296155
1107	32-33	0.19533500482594945
1107	34-35	0.1328096672061463
1107	36-37	0.1684162307319923
1107	38-39	0.12789880144651988
1107	40-41	0.0943728128723933
1107	42-43	0.09349888547129126
1107	44-45	0.1864683966335079
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2216	3	-5.930821249048158
2216	4	-3.1829023930947855
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2216	90-91	-0.9302285232660985
2216	92-93	-1.0186735601731343
2216	94-95	-1.0805062832831247
2216	96-97	-1.0389272750713872
2216	98-99	-1.1116006831843919
2216	100	-1.163178181899955
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	4310.0
3	2232.0
4	2474.0
5	3150.0
6	2756.0
7	2257.0
8	2318.0
9	2722.0
10	3169.0
11	6137.0
12	4640.0
13	6158.0
14	6159.0
15	6366.0
16	7812.0
17	9725.0
18	13673.0
19	23129.0
20	48903.0
21	66327.0
22	31166.0
23	19843.0
24	19264.0
25	20887.0
26	21544.0
27	23926.0
28	27675.0
29	32690.0
30	38742.0
31	48478.0
32	62481.0
33	84601.0
34	123328.0
35	232001.0
36	353902.0
37	639204.0
38	886896.0
39	343895.0
40	2248.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	14.80781897697772	40.68440312469767	27.152866062881557	17.35491183544306
2	18.13420106915241	38.18903114167599	25.78095296384702	17.89581482532458
3	19.481321443178462	25.59406497243904	21.330457174560145	33.594156409822354
4	31.251444154618145	26.48601193381416	23.291233008401118	18.971310903166575
5	18.025520915065794	37.0718660763601	24.393238823324438	20.50937418524967
6	34.30835651188624	23.653491857748143	24.626960188904693	17.41119144146092
7	19.434181765161615	23.832165447295615	37.80358137988897	18.930071407653802
8	33.21601958242771	23.94009862880994	24.39107645277321	18.452805335989137
9	32.95644244325631	25.782716357530056	23.962896192621496	17.29794500659214
10-11	17.452075690383133	40.52263878403108	24.093904957018253	17.931380568567533
12-13	17.3919617890589	25.383990673386904	31.464097852827827	25.759949684726376
14-15	17.68834556411305	32.669063396997636	24.030794627930167	25.611796410959144
16-17	25.441745888053614	25.054758248953142	31.46049644698856	18.042999416004683
18-19	17.955398327190142	25.241073425454434	31.4942629220175	25.309265325337915
20-21	25.60077487351234	32.875222783085306	23.900835095801938	17.623167247600414
22-23	25.221550582234435	25.386338867361264	31.57477301259366	17.81733753781064
24-25	17.752228168398005	32.78743773917363	23.95824709593635	25.502086996492018
26-27	18.115183391636258	28.47534657392568	35.618958356759535	17.790511677678523
28-29	24.97842245769597	25.164264253688877	32.075966541062826	17.78134674755233
30-31	23.008409950080306	26.264450498749	32.442889231661795	18.284250319508903
32-33	19.809544786306674	27.4308115901109	33.393206161366564	19.366437462215863
34-35	19.734109003199144	27.17525414597438	33.53588502852539	19.554751822301085
36-37	19.678882913041694	27.29328413840445	33.797401170681134	19.230431777872724
38-39	20.22049969653525	27.09707736180768	33.44045610697901	19.241966834678056
40-41	19.436856151398313	27.983254646089666	33.292457979837984	19.287431222674037
42-43	19.570316808173637	27.32690998883823	33.60728007876567	19.495493124222456
44-45	19.510054188373758	27.692185076704433	33.20445387871708	19.593306856204727
46-47	19.897513380394923	27.3090265362065	33.40258758943754	19.39087249396104
48-49	19.590154375597084	27.562357654949032	33.30310025493714	19.54438771451674
50-51	19.90581958356407	27.972726760167415	32.797322106446885	19.324131549821626
52-53	20.062250652556806	27.813477879486552	32.59679380255228	19.527477665404362
54-55	20.054183547539576	28.55426618208329	31.371455402831817	20.02009486754532
56-57	20.44182741154385	29.099407537882637	30.319192479601774	20.139572570971733
58-59	21.17878852844196	29.346580360402996	29.461635127646073	20.012995983508972
60-61	20.94029794493252	29.66968532957015	29.208374858741525	20.181641866755804
62-63	20.3816163445007	29.78508247517744	29.270165768982388	20.563135411339474
64-65	20.63696402518754	29.71739316803718	29.501585999643183	20.144056807132095
66-67	20.549125121666425	29.255567537474647	29.959576084130685	20.235731256728243
68-69	20.813590960327	29.265646285982893	29.839608489685098	20.081154264005004
70-71	19.932576726643948	29.917084882607845	29.887998091980684	20.262340298767526
72-73	19.891125977898355	29.34203242217282	30.129571918083286	20.637269681845535
74-75	20.207773520406423	29.548129122381688	29.595223300389033	20.648874056822855
76-77	20.642908534620684	29.29643417950788	29.750151295674844	20.310505990196596
78-79	20.564145375091762	29.598569260543623	29.226656713983107	20.610628650381503
80-81	20.73258986876993	29.921814961818853	29.02043562111266	20.32515954829855
82-83	20.777559982514592	29.949721731796718	28.97032269225838	20.302395593430305
84-85	20.591257305298615	30.328210948664736	28.542394055793054	20.538137690243598
86-87	20.6656670626672	30.43472888122872	28.582953976561566	20.31665007954251
88-89	21.446455878037103	29.97758761835622	28.241330841352465	20.33462566225421
90-91	21.070442254571056	30.51209456496437	28.119008189910904	20.29845499055367
92-93	20.68287277148552	30.575528399661955	28.072381411972934	20.669217416879594
94-95	20.870429171740987	30.736192130337614	27.986419663837403	20.406959034083993
96-97	20.8455540298833	30.20239435286034	28.289593705188526	20.66245791206783
98-99	21.239100029887794	30.414127427031694	27.663276551674603	20.68349599140591
100	20.89502857667139	30.933238927292827	27.548306637986506	20.623425858049274
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	51106.0
1	27846.5
2	3470.5
3	2008.0
4	1629.0
5	1535.5
6	1542.5
7	1761.0
8	1677.5
9	1478.5
10	1581.0
11	2504.0
12	21890.5
13	24918.0
14	8703.5
15	8383.5
16	9451.0
17	10532.0
18	11564.0
19	13142.0
20	14957.5
21	16954.5
22	19415.0
23	23008.5
24	26723.5
25	30343.5
26	36128.0
27	42236.0
28	46419.5
29	50344.5
30	54937.5
31	59346.0
32	64411.0
33	71155.0
34	79141.5
35	84784.0
36	89302.0
37	93833.0
38	96667.5
39	98215.5
40	99649.0
41	101478.5
42	101977.5
43	101768.0
44	102199.5
45	102591.5
46	102186.0
47	103052.5
48	102312.0
49	103558.5
50	118635.0
51	114398.5
52	90807.5
53	81115.0
54	75664.0
55	71006.5
56	65311.0
57	58203.5
58	53731.0
59	46434.0
60	36924.5
61	29858.5
62	23262.5
63	17690.0
64	13429.0
65	10018.0
66	7514.0
67	5994.5
68	4768.5
69	3421.0
70	2389.0
71	1815.5
72	1352.0
73	944.0
74	676.0
75	491.5
76	360.0
77	242.5
78	170.5
79	114.5
80	64.0
81	43.5
82	28.0
83	14.0
84	13.5
85	8.0
86	3.5
87	1.5
88	1.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05965053620611469
2	0.0649328985526945
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	1.3900953543631076E-4
18-19	0.0
20-21	4.4791961418366803E-4
22-23	1.235640314989429E-4
24-25	0.0
26-27	0.0022241525669809722
28-29	8.031662047431289E-4
30-31	0.005838400488325053
32-33	0.0015136593858620505
34-35	0.0022241525669809722
36-37	0.008309681118303911
38-39	0.012464521677455867
40-41	0.003428901874095666
42-43	0.007568296929310252
44-45	0.001683559929173097
46-47	0.0025485081496656975
48-49	0.003567911409531976
50-51	0.009746112984479121
52-53	0.003506129393782505
54-55	0.0013283133386136363
56-57	5.869291496199789E-4
58-59	0.001050294267741015
60-61	0.006610675685193446
62-63	0.0019306879921709831
64-65	0.008356017630116015
66-67	0.011321554386090645
68-69	0.012032047567209565
70-71	0.009993241047477008
72-73	0.0044483051339619445
74-75	0.0017762329527973044
76-77	0.004602760173335624
78-79	0.016681144252357293
80-81	0.009746112984479121
82-83	0.006270874598571353
84-85	0.004757215212709302
86-87	0.007398396385999206
88-89	0.004262959086713531
90-91	0.00671879421275502
92-93	0.011197990354591701
94-95	0.00932908437817019
96-97	0.011228881362466437
98-99	0.002486726133916226
100	0.003305337842596723
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3237188.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.71577505749134
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.8381686539108	57.26759951261317
2	10.56355713882856	14.095118037620862
3	1.9421351278963255	3.8871315097195027
4	0.5327930614875633	1.4218280816958573
5	0.25225388209079896	0.8414656627474343
6	0.15413076608304352	0.6169772111661088
7	0.08560958923754604	0.3998057068835447
8	0.07019692608891871	0.3746593864540517
9	0.04849151214579323	0.2911633934864716
>10	0.46927061802801334	7.179332021614893
>50	0.03145847672297112	1.38165578125685
>100	0.00829762457009163	1.1738142206513436
>500	0.002331168532367265	1.1228334549120815
>1k	0.0011189608948858356	1.3595435931821358
>5k	4.662337062024315E-5	0.2406293867680288
>10k+	1.3987011186072945E-4	8.34644303922766
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	198956	6.1459513627259215	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	53706	1.6590324689205571	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	15668	0.4840003113813594	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7736	0.2389728369189556	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA	4654	0.1437667506490201	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4524	0.13975091962530442	No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3503	0.10821120058519926	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0019152424882336152	0.0	0.0	0.0	0.0
2	0.0019152424882336152	0.0	0.0	0.0	9.267302362420718E-5
3	0.0019152424882336152	0.0	0.0	3.089100787473573E-5	1.235640314989429E-4
4	0.0019461334961083509	0.0	0.0	1.235640314989429E-4	1.235640314989429E-4
5	0.0019461334961083509	0.0	0.0	1.5445503937367865E-4	1.235640314989429E-4
6	0.0019461334961083509	0.0	0.0	1.5445503937367865E-4	1.5445503937367865E-4
7	0.0019461334961083509	0.0	0.0	1.5445503937367865E-4	1.5445503937367865E-4
8	0.0019461334961083509	0.0	0.0	1.8534604724841435E-4	1.8534604724841435E-4
9	0.0019461334961083509	0.0	0.0	1.8534604724841435E-4	2.162370551231501E-4
10-11	0.0019461334961083509	0.0	0.0	1.8534604724841435E-4	2.625735669352537E-4
12-13	0.002038806519732558	0.0	0.0	1.8534604724841435E-4	2.7801907087262153E-4
14-15	0.002146925047294133	0.0	0.0	2.162370551231501E-4	2.7801907087262153E-4
16-17	0.0022087070630436045	0.0	0.0	2.625735669352537E-4	2.7801907087262153E-4
18-19	0.0022859345827304436	0.0	0.0	3.39801086622093E-4	2.9346457480998944E-4
20-21	0.0023631621024172832	0.0	0.0	3.706920944968287E-4	3.2435558268472514E-4
22-23	0.002409498614229387	0.0	0.0	4.324741102463002E-4	3.706920944968287E-4
24-25	0.002486726133916226	0.0	0.0	4.479196141836681E-4	3.706920944968287E-4
26-27	0.0025793991575404334	0.0	0.0	4.6336511812103594E-4	3.706920944968287E-4
28-29	0.0026875176851020085	0.0	0.0	4.788106220584038E-4	3.706920944968287E-4
30-31	0.00274929970085148	0.0	0.0	4.942561259957716E-4	3.706920944968287E-4
32-33	0.0028574182284130546	0.0	0.0	5.405926378078752E-4	4.324741102463002E-4
34-35	0.0030118732677867337	0.0	0.0	6.023746535573467E-4	4.479196141836681E-4
36-37	0.0031508828032230443	0.0	0.0	6.487111653694503E-4	4.6336511812103594E-4
38-39	0.0034597928819704014	0.0	0.0	6.487111653694503E-4	4.788106220584038E-4
40-41	0.004170286063089323	0.0	0.0	6.641566693068181E-4	4.942561259957716E-4
42-43	0.005097016299331395	0.0	0.0	7.259386850562896E-4	4.942561259957716E-4
44-45	0.00607008304738557	0.0	0.0	7.568296929310254E-4	5.097016299331395E-4
46-47	0.006888694756066066	0.0	0.0	7.722751968683932E-4	6.023746535573467E-4
48-49	0.007969880031681817	0.0	0.0	7.877207008057611E-4	6.332656614320824E-4
50-51	0.009128292826984408	0.0	0.0	8.958392283673361E-4	6.487111653694503E-4
52-53	0.011367890897902747	0.0	0.0	8.958392283673361E-4	6.79602173244186E-4
54-55	0.013746498504257399	0.0	0.0	9.112847323047039E-4	6.79602173244186E-4
56-57	0.01728351890591464	0.0	0.0	9.576212441168076E-4	6.79602173244186E-4
58-59	0.021731824039876585	0.0	0.0	0.001019403259866279	6.79602173244186E-4
60-61	0.02770923406363795	0.0	0.0	0.0010966307795531182	6.950476771815538E-4
62-63	0.03493772990632611	0.0	0.0	0.0011120762834904861	7.104931811189217E-4
64-65	0.04428225978843367	0.0	0.0	0.0011120762834904861	7.259386850562896E-4
66-67	0.057040246040699516	0.0	0.0	0.0011120762834904861	7.722751968683932E-4
68-69	0.0742156464190526	0.0	0.0	0.0011429672913652218	7.877207008057611E-4
70-71	0.09865043364796855	0.0	0.0	0.0012201948110520614	8.340572126178646E-4
72-73	0.12924797694789428	0.0	0.0	0.001359204346488372	8.495027165552325E-4
74-75	0.17036390842916754	0.0	0.0	0.0013746498504257397	9.112847323047039E-4
76-77	0.22164298150122885	0.0	0.0	0.0013900953543631077	9.421757401794397E-4
78-79	0.2886919140933427	0.0	0.0	0.0013900953543631077	0.0010657397716783827
80-81	0.3702905113944571	0.0	0.0	0.0014518773701125791	0.0010811852756157504
82-83	0.47345103219213713	0.0	0.0	0.0014518773701125791	0.0011120762834904861
84-85	0.5998570364155558	0.0	0.0	0.0015445503937367865	0.0011429672913652218
86-87	0.7574784040963948	0.0	0.0	0.0015754414016115222	0.0011738582992399577
88	0.8936150758003552	0.0	0.0	0.0015754414016115222	0.0011738582992399577
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	2075	0.0	84.34314	1
AACGAGT	57645	0.0	71.85274	1
ACGAGTG	61020	0.0	70.94623	2
GTGGAAT	64810	0.0	70.194695	6
CGAGTGG	62635	0.0	69.984085	3
GAATCAC	65450	0.0	69.874435	9
TGGAATC	65660	0.0	69.69389	7
GGAATCA	65710	0.0	69.5765	8
AGTGGAA	64545	0.0	68.75758	5
GAGTGGA	64965	0.0	68.30584	4
CGTGTGG	1685	0.0	58.002224	3
AACGTGT	1385	0.0	56.38755	1
ATCGAGT	775	0.0	54.634342	1
ACGTGTG	1730	0.0	53.265522	2
TCGAGTG	955	0.0	53.16877	2
GAGTACT	30195	0.0	41.363884	20-21
AGTACTT	30835	0.0	41.160625	20-21
GTACTTT	30235	0.0	40.339687	22-23
TACTTTT	32985	0.0	37.959522	22-23
CATGGGG	13710	0.0	37.775414	24-25
>>END_MODULE