FastQCFastQC Report
Thu 26 May 2016
SRR2132671_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132671_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5706249
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3505936.144018601361419No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA972981.7051131137109508No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG589351.0328150769445918No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT143980.25231986897171854No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT71470.12524865283656567No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT65590.11494416034070719No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT40250.081.2478941
GTGGAAT1395600.062.776396
TGGAATC1408250.062.5161327
GAATCAC1414300.062.2553569
GGAATCA1418500.062.0908978
AGTGGAA1386050.061.8561865
AACGAGT1296650.061.6979061
ACGAGTG1361400.061.397462
CGAGTGG1393100.060.8415373
ATCGAGT17300.060.3809661
GAGTGGA1431050.059.9242254
AACGTGT23900.059.6537741
TCGAGTG20750.055.9764562
ACGTGTG29450.055.7271272
CGTGTGG31800.055.7099043
GAGTACT532650.041.36445220-21
AGTACTT545700.041.1675220-21
GTACTTT540950.040.131622-23
CGATTGG9800.038.839813
TACTTTT577900.038.4479722-23
Produced by FastQC (version 0.11.5)