FastQCFastQC Report
Thu 26 May 2016
SRR2132670_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132670_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4502116
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2638075.859622453086504No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG557591.2385065156028854No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA548901.2192044807375022No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT105130.23351241949341153No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT52520.1166562567468275No Hit
GTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGT51630.11467940852701262No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT47740.10603902698197913No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT30700.083.981241
ATCGAGT13550.064.235121
TCGAGTG16100.061.037262
GTGGAAT1149650.060.4725886
TGGAATC1163900.059.9542587
GAATCAC1168700.059.6316229
AGTGGAA1147050.059.3315125
GGAATCA1176350.059.295768
ACGAGTG1132300.058.5548672
AACGAGT1085250.058.525361
CGAGTGG1159350.058.0737953
AACGTGT19400.057.9610561
GAGTGGA1195350.056.9891624
CGTGTGG25450.055.0221673
ACGTGTG23500.054.0219762
GAGTACT417800.040.9293620-21
AGTACTT426000.040.75372720-21
GTACTTT417800.040.14315422-23
TACTTTT448300.038.05145322-23
CATGGGG221900.036.95731724-25