FastQCFastQC Report
Thu 26 May 2016
SRR2132669_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132669_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4098334
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2466086.017274336352283No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1151912.810678680654139No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG321810.785221507080682No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT97910.23890195381830762No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT55210.13471327617514825No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA51290.12514841396528445No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA48910.11934117619500997No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC46600.11370473953562595No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT45280.11048391858740648No Hit
GTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGT42240.1030662703430223No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT26000.084.506941
GTGGAAT911600.064.1462556
AACGAGT829650.063.911331
ACGAGTG872700.063.630982
TGGAATC924200.063.4751177
GAATCAC929150.063.243179
GGAATCA931800.063.0431378
TCGAGTG14050.062.91662
CGAGTGG900000.062.712053
AGTGGAA915400.062.478445
ATCGAGT12250.061.4515381
GAGTGGA932900.061.3467254
CGTGTGG19950.057.006323
AACGTGT15400.054.0756031
ACGTGTG19950.049.9662442
AGTACTT380750.040.6635620-21
CGATTGG5700.040.39913
GAGTACT374000.040.31053520-21
GTACTTT381200.038.98869722-23
TACTTTT412700.037.0604722-23