##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132669_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4098334
Sequences flagged as poor quality	0
Sequence length	100
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.179497571452206	31.0	28.0	34.0	16.0	34.0
2	30.305721544412926	33.0	30.0	34.0	19.0	34.0
3	30.76426494277919	33.0	31.0	34.0	25.0	34.0
4	35.222789309021664	37.0	35.0	37.0	32.0	37.0
5	34.67829561963471	37.0	35.0	37.0	32.0	37.0
6	35.22778572951839	37.0	35.0	37.0	32.0	37.0
7	35.63824519914677	37.0	35.0	37.0	33.0	37.0
8	35.832259645016734	37.0	35.0	37.0	35.0	37.0
9	37.744635259107724	39.0	39.0	39.0	35.0	39.0
10-11	37.82464813751149	39.0	39.0	39.0	35.0	39.0
12-13	37.809933011804304	39.0	39.0	39.0	35.0	39.0
14-15	39.228749535786974	41.0	39.0	41.0	36.0	41.0
16-17	39.16178317823779	41.0	39.0	41.0	36.0	41.0
18-19	39.04878250528141	41.0	39.0	41.0	35.5	41.0
20-21	38.902628482695654	41.0	39.0	41.0	35.0	41.0
22-23	38.813994784222075	40.5	39.0	41.0	35.0	41.0
24-25	38.72784221588577	41.0	39.0	41.0	35.0	41.0
26-27	38.67720969057183	41.0	39.0	41.0	35.0	41.0
28-29	38.522137287980925	41.0	39.0	41.0	35.0	41.0
30-31	38.403853248661534	40.5	38.5	41.0	35.0	41.0
32-33	38.279246884221735	40.0	38.0	41.0	35.0	41.0
34-35	38.20223144331331	40.0	38.0	41.0	34.0	41.0
36-37	38.04838929672398	40.0	38.0	41.0	34.0	41.0
38-39	37.664865406284605	40.0	36.5	41.0	34.0	41.0
40-41	37.48823143745727	40.0	36.0	41.0	33.0	41.0
42-43	37.3591752160756	40.0	35.0	41.0	33.0	41.0
44-45	37.22921594482051	40.0	35.0	41.0	33.0	41.0
46-47	37.09212694719366	40.0	35.0	41.0	33.0	41.0
48-49	36.94345055820243	40.0	35.0	41.0	33.0	41.0
50-51	36.6995691419977	40.0	35.0	41.0	32.0	41.0
52-53	36.26388503230825	39.5	35.0	41.0	30.5	41.0
54-55	35.539720042339155	39.0	35.0	41.0	28.0	41.0
56-57	34.96993449045392	39.0	35.0	41.0	23.5	41.0
58-59	34.672811073963224	39.0	35.0	41.0	22.0	41.0
60-61	34.433155521243506	39.0	35.0	41.0	21.5	41.0
62-63	34.14995349329752	38.0	35.0	40.0	20.0	41.0
64-65	33.88223080402915	37.5	34.5	40.0	19.0	41.0
66-67	33.56720450309808	37.0	34.0	40.0	18.0	41.0
68-69	33.28038173560281	36.5	34.0	40.0	16.0	41.0
70-71	32.948445758691214	36.0	34.0	39.0	12.0	41.0
72-73	32.59542743466004	35.5	34.0	39.0	8.0	41.0
74-75	32.24156193711884	35.0	33.0	38.5	7.0	40.0
76-77	31.868661997777636	35.0	33.0	37.0	2.0	39.5
78-79	31.734121596726865	35.0	33.0	37.0	2.0	39.0
80-81	31.583414626528732	35.0	34.0	37.0	2.0	39.0
82-83	31.247817283803613	35.0	33.0	36.0	2.0	39.0
84-85	30.95042558756802	35.0	33.0	36.0	2.0	37.0
86-87	30.748446929898833	35.0	33.0	36.0	2.0	37.0
88-89	30.52179958978453	35.0	33.0	35.0	2.0	36.5
90-91	30.312063755662667	35.0	33.0	35.0	2.0	36.0
92-93	30.1434552430329	35.0	33.0	35.0	2.0	36.0
94-95	29.955865358948294	35.0	33.0	35.0	2.0	36.0
96-97	29.770747088939068	35.0	33.0	35.0	2.0	36.0
98-99	29.62574682785737	35.0	33.0	35.0	2.0	35.0
100	29.523310203609565	35.0	33.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	2.931274410767415
1101	2	2.351811979675958
1101	3	2.1079636150701155
1101	4	1.0492534125787714
1101	5	1.6307286427923984
1101	6	1.0652303843260427
1101	7	0.566592527968929
1101	8	0.4783155228313376
1101	9	0.43625915614340727
1101	10-11	0.3854599305612183
1101	12-13	0.3332297443265304
1101	14-15	0.5060799365803916
1101	16-17	0.4406361319433927
1101	18-19	0.5129442174348142
1101	20-21	0.5510201856109731
1101	22-23	0.5191500800896733
1101	24-25	0.5894206999526403
1101	26-27	0.5824249022210495
1101	28-29	0.6090738379564371
1101	30-31	0.5683222613405476
1101	32-33	0.5412087553343667
1101	34-35	0.5771975524703521
1101	36-37	0.5704518420194802
1101	38-39	0.5117464166951109
1101	40-41	0.5278995041972223
1101	42-43	0.5576893532069462
1101	44-45	0.5375718890451324
1101	46-47	0.5644941660627723
1101	48-49	0.5150377335358982
1101	50-51	0.5114998953954881
1101	52-53	0.41883593689743037
1101	54-55	0.44746381901184407
1101	56-57	0.3888722992228608
1101	58-59	0.3619930641907487
1101	60-61	0.3511398663356573
1101	62-63	0.37881585106180893
1101	64-65	0.37544385664921975
1101	66-67	0.28130572564782597
1101	68-69	0.3159579494406657
1101	70-71	0.38720464424155665
1101	72-73	0.3684052987378692
1101	74-75	0.39414365676017127
1101	76-77	0.3369514832480043
1101	78-79	0.37629455741839735
1101	80-81	0.4080481901153483
1101	82-83	0.437688213564261
1101	84-85	0.41581346999121394
1101	86-87	0.41557226646011713
1101	88-89	0.3665039387197524
1101	90-91	0.31840048740340166
1101	92-93	0.403352951849115
1101	94-95	0.3760404967613127
1101	96-97	0.35848353202379
1101	98-99	0.3742425623753931
1101	100	0.41020795937169297
1102	1	2.7777346307191237
1102	2	2.2691090553204134
1102	3	2.0666555141272447
1102	4	1.0340636300286619
1102	5	1.5480924974743218
1102	6	0.9748942685510542
1102	7	0.5000679128901666
1102	8	0.39030242024251294
1102	9	0.35817276704901246
1102	10-11	0.3473271714820143
1102	12-13	0.33529163259969863
1102	14-15	0.4876919415089631
1102	16-17	0.4313802865138001
1102	18-19	0.5248037887516972
1102	20-21	0.5112577553880229
1102	22-23	0.4914553277538687
1102	24-25	0.5525403145590815
1102	26-27	0.4819765361093147
1102	28-29	0.5678016160416846
1102	30-31	0.5804671140186386
1102	32-33	0.5528689196852099
1102	34-35	0.5852839850627731
1102	36-37	0.5806996748673612
1102	38-39	0.49121313478610773
1102	40-41	0.4947345200604616
1102	42-43	0.537955778312778
1102	44-45	0.46810823031495374
1102	46-47	0.4575559088774668
1102	48-49	0.4753517257796318
1102	50-51	0.42332200643542706
1102	52-53	0.434422946659339
1102	54-55	0.39326114006446744
1102	56-57	0.40173187363111396
1102	58-59	0.3670723472678503
1102	60-61	0.37544119852137214
1102	62-63	0.344165149609708
1102	64-65	0.3055131960513293
1102	66-67	0.2472126879766563
1102	68-69	0.29462855799467036
1102	70-71	0.25360629944248103
1102	72-73	0.23041270997995156
1102	74-75	0.3017933227131593
1102	76-77	0.2113736674357476
1102	78-79	0.2683721780230215
1102	80-81	0.36898163248007165
1102	82-83	0.4492830761689248
1102	84-85	0.3972238383381992
1102	86-87	0.3905909152107796
1102	88-89	0.3790758380077719
1102	90-91	0.4005071879397981
1102	92-93	0.44997902784037436
1102	94-95	0.39613536512467107
1102	96-97	0.3917280847105218
1102	98-99	0.3851457380510155
1102	100	0.34675884588735073
1103	1	2.5730465720031326
1103	2	2.1753450692193894
1103	3	1.982407720364595
1103	4	0.940111361701824
1103	5	1.5339034728847096
1103	6	0.9233540779010383
1103	7	0.47439892118393345
1103	8	0.35404519774731114
1103	9	0.369646628583574
1103	10-11	0.2484324344749993
1103	12-13	0.2081833356144358
1103	14-15	0.37683839104470707
1103	16-17	0.38580518259237806
1103	18-19	0.29668506345905143
1103	20-21	0.3872475726696436
1103	22-23	0.35274925001914426
1103	24-25	0.44927236067324827
1103	26-27	0.4311537513517223
1103	28-29	0.44455745625185017
1103	30-31	0.39806946426069345
1103	32-33	0.4208657400694591
1103	34-35	0.4197800791907085
1103	36-37	0.3248520201727345
1103	38-39	0.3269593066731815
1103	40-41	0.2960568579188987
1103	42-43	0.3367758049540015
1103	44-45	0.24156210747605655
1103	46-47	0.2484395089814413
1103	48-49	0.20943939395795041
1103	50-51	0.2802742171609154
1103	52-53	0.21525808424127035
1103	54-55	0.18821518176832797
1103	56-57	0.10731042345735631
1103	58-59	0.12039413873025495
1103	60-61	0.127766173375349
1103	62-63	0.05143536073993005
1103	64-65	0.07481515690454188
1103	66-67	0.13424744965288937
1103	68-69	0.0955928757680411
1103	70-71	0.04112139904233203
1103	72-73	0.08610474731280249
1103	74-75	0.05252934661404396
1103	76-77	0.05906700397219211
1103	78-79	0.04638051144628719
1103	80-81	0.024879422572677612
1103	82-83	0.08451132866805366
1103	84-85	0.13685715582468916
1103	86-87	0.10090644606824384
1103	88-89	0.11600367300023962
1103	90-91	0.11801987449578988
1103	92-93	0.13383854942999562
1103	94-95	0.09170849946030302
1103	96-97	0.09258435510447072
1103	98-99	0.06055092978276022
1103	100	0.11657261786855244
1104	1	1.5387134916293697
1104	2	1.7396599489454516
1104	3	1.8166662720212763
1104	4	0.8656984864098476
1104	5	1.5166400145232686
1104	6	0.9829087540487507
1104	7	0.5204456136839397
1104	8	0.3872193374900377
1104	9	0.3039199532047263
1104	10-11	0.2666214901548116
1104	12-13	0.2646229340804567
1104	14-15	0.3983154637540878
1104	16-17	0.3529268027213277
1104	18-19	0.3875296337333296
1104	20-21	0.43752690768131686
1104	22-23	0.38235466811103436
1104	24-25	0.36231187511215524
1104	26-27	0.3497063103025724
1104	28-29	0.4589414542143757
1104	30-31	0.515599857962286
1104	32-33	0.47543370430528853
1104	34-35	0.41420842113872425
1104	36-37	0.48757008149141257
1104	38-39	0.5174167975724657
1104	40-41	0.4968510066958203
1104	42-43	0.49153244859293466
1104	44-45	0.4937604484815523
1104	46-47	0.5430618075114779
1104	48-49	0.4858204269770994
1104	50-51	0.5017141980198403
1104	52-53	0.557516371281011
1104	54-55	0.5232385715112784
1104	56-57	0.4782193225165585
1104	58-59	0.47218996611532305
1104	60-61	0.4509117154608049
1104	62-63	0.3826685389153255
1104	64-65	0.4140007483607704
1104	66-67	0.4128772561526688
1104	68-69	0.4161377801695565
1104	70-71	0.4647175975706972
1104	72-73	0.42365612531517627
1104	74-75	0.5047406231116156
1104	76-77	0.5256182888724688
1104	78-79	0.47562173623482096
1104	80-81	0.524980954031431
1104	82-83	0.5888182149971009
1104	84-85	0.5589267624158367
1104	86-87	0.5409324446038042
1104	88-89	0.5770840079732871
1104	90-91	0.5536222823739969
1104	92-93	0.5318590662861524
1104	94-95	0.5258042845181663
1104	96-97	0.5953893625853084
1104	98-99	0.5835882596420916
1104	100	0.5193449742750502
1105	1	2.241129162472326
1105	2	2.010060570379345
1105	3	1.9456191454872531
1105	4	0.9261961468943269
1105	5	1.5113098003077
1105	6	0.9829143991213201
1105	7	0.4600627279036047
1105	8	0.3173506034003495
1105	9	0.27427344967407663
1105	10-11	0.22262740183681018
1105	12-13	0.17102925101285393
1105	14-15	0.3124297349076386
1105	16-17	0.22619131629150502
1105	18-19	0.21197424339529647
1105	20-21	0.33055242811777674
1105	22-23	0.3040337415333738
1105	24-25	0.33948454330101185
1105	26-27	0.24326806200453888
1105	28-29	0.2320408633347526
1105	30-31	0.28222406507639874
1105	32-33	0.25136730691111353
1105	34-35	0.25003299081332386
1105	36-37	0.22512939209185845
1105	38-39	0.18761970983249654
1105	40-41	0.13040729761279124
1105	42-43	0.15491708778850466
1105	44-45	0.27337623319803583
1105	46-47	0.31079748638006066
1105	48-49	0.22389438846474263
1105	50-51	0.2153161117551292
1105	52-53	0.20623544102976155
1105	54-55	0.2099284624621447
1105	56-57	0.2258945905018308
1105	58-59	0.19885375506069636
1105	60-61	0.299662109033747
1105	62-63	0.35228754309515864
1105	64-65	0.3194002172379129
1105	66-67	0.29894479430716103
1105	68-69	0.22037270095298567
1105	70-71	0.25069406273155437
1105	72-73	0.27073297004261576
1105	74-75	0.31492038753641083
1105	76-77	0.18580127326690743
1105	78-79	0.24891328033633187
1105	80-81	0.2929851662175089
1105	82-83	0.42182146347056104
1105	84-85	0.3471112103776086
1105	86-87	0.24856178296231946
1105	88-89	0.2979315115779073
1105	90-91	0.31444307643167235
1105	92-93	0.3249995588777175
1105	94-95	0.3555733620662238
1105	96-97	0.40210683693022986
1105	98-99	0.3797084139231899
1105	100	0.35576446624990865
1106	1	1.7001468969555518
1106	2	1.0527110798424033
1106	3	1.5302867769892536
1106	4	0.7945219647206301
1106	5	1.4952805488430627
1106	6	0.9649029266516891
1106	7	0.5234907856112088
1106	8	0.3645472711861686
1106	9	0.2834504383876961
1106	10-11	0.19709663401771849
1106	12-13	0.20007319251001832
1106	14-15	0.32415504170167253
1106	16-17	0.26141624582929524
1106	18-19	0.2499968639892316
1106	20-21	0.35017320470333857
1106	22-23	0.27783624499203086
1106	24-25	0.3372032594818677
1106	26-27	0.28330882177188954
1106	28-29	0.32061981928464434
1106	30-31	0.23079765919127482
1106	32-33	0.25548679872020585
1106	34-35	0.23761924638037613
1106	36-37	0.19583453392777983
1106	38-39	0.14639120734249644
1106	40-41	0.13975651120910015
1106	42-43	0.12442827567197412
1106	44-45	0.15942057466922677
1106	46-47	0.11116181451181717
1106	48-49	0.09605435833577758
1106	50-51	-0.05406231139521367
1106	52-53	0.09222677433429283
1106	54-55	0.047597813587486826
1106	56-57	-0.025708569237075096
1106	58-59	-0.1403010037708725
1106	60-61	-0.06603209216454076
1106	62-63	0.005534347393108874
1106	64-65	-0.0919719392069922
1106	66-67	-0.07264872599205319
1106	68-69	-0.03685816117226892
1106	70-71	-0.02709872923227863
1106	72-73	-0.07193853605127742
1106	74-75	-0.09035625516917634
1106	76-77	-0.15639075262742708
1106	78-79	-0.08270917724382798
1106	80-81	-0.08363878169314987
1106	82-83	-0.05682060902744723
1106	84-85	-0.09830800278185592
1106	86-87	-0.09302932164254685
1106	88-89	-0.15227866258181422
1106	90-91	-0.12229498890905788
1106	92-93	-0.12314955663222094
1106	94-95	-0.10386431718883316
1106	96-97	-0.09514352092048028
1106	98-99	-0.1521021138609271
1106	100	-0.18297392868711526
1107	1	1.6472206856895504
1107	2	1.0881413118869396
1107	3	1.4254235740826324
1107	4	0.7198630818464125
1107	5	1.4519555169245564
1107	6	1.0066261406267003
1107	7	0.5430944312883383
1107	8	0.4281774577002935
1107	9	0.4023648699267852
1107	10-11	0.22940462452373822
1107	12-13	0.16482300562056196
1107	14-15	0.2772880830952147
1107	16-17	0.28376937689886006
1107	18-19	0.26884421060126584
1107	20-21	0.23385295043329535
1107	22-23	0.25161639476571906
1107	24-25	0.3460942362713766
1107	26-27	0.30761289593008456
1107	28-29	0.29170565438221274
1107	30-31	0.19834621768713845
1107	32-33	0.2510017489139784
1107	34-35	0.17533852440821818
1107	36-37	0.24773902253591729
1107	38-39	0.24165585921123522
1107	40-41	0.25464748833160655
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2216	3	-5.873040629090518
2216	4	-2.9501097559550047
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2216	90-91	-0.9766789492779218
2216	92-93	-1.0498730062721329
2216	94-95	-1.1049757559269509
2216	96-97	-1.1229998876286302
2216	98-99	-1.130260472840959
2216	100	-1.243822700215528
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	6464.0
3	6101.0
4	6592.0
5	8979.0
6	7451.0
7	5463.0
8	4836.0
9	5177.0
10	5508.0
11	9531.0
12	7053.0
13	9044.0
14	8921.0
15	9390.0
16	11177.0
17	13745.0
18	18754.0
19	30569.0
20	62580.0
21	82815.0
22	39611.0
23	24881.0
24	24558.0
25	26684.0
26	28274.0
27	30733.0
28	35838.0
29	41947.0
30	50313.0
31	62717.0
32	81240.0
33	110185.0
34	159022.0
35	332871.0
36	440806.0
37	776910.0
38	1075205.0
39	433204.0
40	3185.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	13.76095831330296	41.0712711826543	26.844232827123367	18.32353767691937
2	17.346034121016242	38.5373421567883	26.930517367545015	17.186106354650445
3	18.378931536570715	26.53336697301879	21.319955864992945	33.76774562541755
4	30.616709131076185	27.212423389601724	23.708536200319447	18.46233127900264
5	17.592197219650714	38.027037327850785	24.663314410196925	19.71745104230158
6	33.91407337713325	24.307316094783882	24.959678737750508	16.818931790332364
7	18.780631349226297	24.77162671466015	38.27072171277402	18.177020223339532
8	32.530169576222924	24.667852839714868	24.8706425586592	17.931335025403005
9	32.315399379357565	26.201744416145683	24.805811336996936	16.67704486749982
10-11	16.829960662064146	41.282921304120165	24.428706884309577	17.458411149506116
12-13	16.821591407630514	25.994953071174777	31.7403608393069	25.443094681887814
14-15	17.16739045670753	33.4483841482905	24.05312744154088	25.33109795346109
16-17	25.07525628127952	25.714675420152943	31.569537243105668	17.64053105546187
18-19	17.451664505625946	25.9650262765309	31.62198346938048	24.961325748462666
20-21	25.260756141264174	33.686351839405475	23.94920701072378	17.103685008606572
22-23	24.83810789829959	26.109051939795503	31.61229148984166	17.44054867206325
24-25	17.310485187395656	33.626212504886134	23.878251504147784	25.185050803570423
26-27	17.713050057988493	29.351802620987076	35.59013446546519	17.345012855559244
28-29	24.80819462727793	25.89812348793828	31.995890974173406	17.297790910610388
30-31	22.430735398865178	27.383275473031453	32.27634880550422	17.909640322599145
32-33	19.328754170962174	28.29980906600948	33.18889044781036	19.182546315217987
34-35	19.51497769349779	28.164486037863284	33.2992211665263	19.021315102112624
36-37	19.507159650175737	28.081060507435275	33.678241376495684	18.733538465893307
38-39	20.06642307391022	27.91230855993536	33.25642664409859	18.764841722055834
40-41	18.95093031640091	29.226630455669284	33.08441932774186	18.73801990018795
42-43	18.96943608234077	28.13454844091114	33.63656118194683	19.259454294801255
44-45	18.945280116810448	28.861248184749467	32.86851858110148	19.3249531173386
46-47	19.671258721431016	28.26667216496455	33.200438822733084	18.861630290871346
48-49	19.08663281023623	28.444550182245198	33.22101538790014	19.24780161961843
50-51	19.65395548642599	28.981835023979112	32.53858462091085	18.825624868684056
52-53	19.67716196316174	28.687797158986232	32.52879018394728	19.10625069390474
54-55	19.647421788911803	29.606848551632055	30.949732911561668	19.795996747894478
56-57	19.90386766834809	30.139735681228526	30.20080965100484	19.75558699941854
58-59	21.081012430285067	30.061254711408175	29.23825432856623	19.619478529740526
60-61	20.553354716629837	30.699720367583822	28.907127606889944	19.839797308896394
62-63	19.82650458828421	30.737221906641448	28.968180185561497	20.468093319512846
64-65	20.298032786925248	30.645615287729605	29.246056404755922	19.810295520589225
66-67	20.283006551418765	30.045760123927163	29.833053148558612	19.83818017609546
68-69	20.6746574276924	30.132331346319386	29.482003705594934	19.711007520393277
70-71	19.419715792610063	31.117629589428596	29.54301483946868	19.919639778492666
72-73	19.449705959109156	30.192419539087656	29.9533363127232	20.404538189079986
74-75	19.72452652386273	30.665310316371393	29.157082298237807	20.45308086152807
76-77	20.358694344582094	30.06131316614045	29.51635875876403	20.06363373051342
78-79	20.056329723874047	30.539803336490422	29.008746213386715	20.395120726248816
80-81	20.559763472730232	30.958551846368547	28.585104355505408	19.896580325395814
82-83	20.395705445497743	30.882742830259147	28.771872930048715	19.949678794194394
84-85	20.21916053329104	31.277244845981865	28.148433282192215	20.355161338534884
86-87	20.156563127016593	31.519420013652912	28.381837359558958	19.942179499771537
88-89	21.31238585563331	30.778768833629872	28.016708496470788	19.89213681426603
90-91	20.70182951751737	31.462991770526404	27.815169317301415	20.02000939465481
92-93	20.134855210985496	31.642156614529497	27.752568921977026	20.470419252507977
94-95	20.49686167134562	31.71925846545898	27.66811010791585	20.115769755279544
96-97	20.50632812545755	31.073641205145275	28.14062387137204	20.279406798025132
98-99	21.109169486279256	31.216780616307755	27.394995179014543	20.27905471839845
100	20.296958553907004	32.115786995069804	27.383873222854337	20.203381228168848
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	116660.0
1	63254.5
2	7261.0
3	3860.0
4	2879.5
5	2545.5
6	2628.5
7	3129.5
8	2927.0
9	2515.0
10	2565.5
11	3803.0
12	30375.5
13	33612.5
14	10411.5
15	10004.5
16	11442.0
17	13031.0
18	14428.5
19	15997.0
20	18138.5
21	20968.0
22	24287.0
23	28672.0
24	33727.5
25	38764.0
26	46356.0
27	54582.5
28	59908.0
29	65052.0
30	70794.5
31	76150.0
32	82114.0
33	88668.5
34	95756.0
35	102340.0
36	108646.5
37	114049.0
38	117225.0
39	119517.5
40	122953.0
41	125966.5
42	125005.0
43	123160.5
44	122754.5
45	121496.0
46	121867.0
47	125389.0
48	126561.0
49	135449.0
50	174768.5
51	171469.5
52	121381.0
53	100119.5
54	93400.0
55	88986.0
56	81153.5
57	69985.0
58	61678.0
59	51151.5
60	39493.0
61	31934.0
62	24778.0
63	18554.0
64	13935.5
65	10030.0
66	7791.5
67	6375.0
68	5136.5
69	3997.0
70	2963.5
71	2262.5
72	1781.5
73	1238.5
74	814.5
75	615.0
76	454.5
77	298.0
78	195.0
79	117.5
80	64.5
81	35.0
82	23.0
83	13.0
84	7.5
85	5.5
86	3.0
87	2.0
88	1.0
89	0.5
90	0.0
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06029279214432011
2	0.066734434040759
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	1.2200079349316086E-4
18-19	0.0
20-21	3.0500198373290216E-4
22-23	6.100039674658043E-5
24-25	0.0
26-27	0.002610816980753643
28-29	9.516061892466548E-4
30-31	0.005978038881164883
32-33	0.0014396093632192985
34-35	0.00218381420352758
36-37	0.00813745292599383
38-39	0.012468481095001042
40-41	0.0032086208688701314
42-43	0.007698250069418452
44-45	0.0017202111882535685
46-47	0.002342415235068689
48-49	0.0035746232493496133
50-51	0.009589262368562446
52-53	0.0036356236460961945
54-55	0.0016104104741097235
56-57	6.100039674658043E-4
58-59	9.028058718493904E-4
60-61	0.006722243721473165
62-63	0.001708011108904252
64-65	0.00801545213250067
66-67	0.010809270303494054
68-69	0.011626675619898231
70-71	0.010028465225137824
72-73	0.004379828486404475
74-75	0.001976412854589206
76-77	0.00470923062883601
78-79	0.01647010712157672
80-81	0.009052458877192537
82-83	0.0063562413409936815
84-85	0.004794631184281223
86-87	0.007478648641130762
88-89	0.004379828486404475
90-91	0.0063196411029457335
92-93	0.011163072604624222
94-95	0.009089059115240486
96-97	0.010687269510000893
98-99	0.0025742167427056945
100	0.002903618885137229
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4098334.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.92714194586739
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.85119720055883	49.05108313077072
2	13.015901201147928	15.600115176691553
3	2.9277538549211983	5.263557625392693
4	0.8472064309992592	2.0308264019177726
5	0.35232075296603266	1.055678788673516
6	0.2047280250837273	0.7361259251693776
7	0.13095033240236123	0.5493235410414373
8	0.08756114505735449	0.4197831335035813
9	0.06226541992355899	0.3358249792270343
>10	0.42363699554781215	5.831773574865169
>50	0.07175353929122485	2.8175361368265603
>100	0.020333542464048157	2.0926920290608653
>500	0.0014364914794681952	0.6252955992147438
>1k	0.002708812477483156	3.39742381975537
>5k	1.231278398855979E-4	0.5027599908754695
>10k+	1.231278398855979E-4	9.690200147014211
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	246608	6.017274336352283	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	115191	2.810678680654139	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	32181	0.785221507080682	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	9791	0.23890195381830762	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5521	0.13471327617514825	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA	5129	0.12514841396528445	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4891	0.11934117619500997	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC	4660	0.11370473953562595	No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4528	0.11048391858740648	No Hit
GTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGT	4224	0.1030662703430223	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.00527043427890455	0.0	0.0	2.4400158698632174E-5	2.4400158698632174E-5
2	0.00527043427890455	0.0	0.0	4.880031739726435E-5	2.4400158698632174E-5
3	0.00527043427890455	0.0	0.0	4.880031739726435E-5	2.4400158698632174E-5
4	0.00527043427890455	2.4400158698632174E-5	0.0	7.320047609589653E-5	2.4400158698632174E-5
5	0.00527043427890455	4.880031739726435E-5	0.0	7.320047609589653E-5	2.4400158698632174E-5
6	0.005294834437603182	9.76006347945287E-5	0.0	9.76006347945287E-5	2.4400158698632174E-5
7	0.005294834437603182	9.76006347945287E-5	0.0	9.76006347945287E-5	2.4400158698632174E-5
8	0.005294834437603182	9.76006347945287E-5	0.0	9.76006347945287E-5	2.4400158698632174E-5
9	0.005294834437603182	9.76006347945287E-5	0.0	9.76006347945287E-5	2.4400158698632174E-5
10-11	0.005294834437603182	9.76006347945287E-5	0.0	9.76006347945287E-5	3.6600238047948265E-5
12-13	0.005307034516952499	1.098007141438448E-4	0.0	1.2200079349316088E-4	7.320047609589653E-5
14-15	0.00533143467565113	1.3420087284247696E-4	0.0	1.2200079349316088E-4	1.2200079349316088E-4
16-17	0.005355834834349763	1.5860103154110916E-4	0.0	1.2200079349316088E-4	1.2200079349316088E-4
18-19	0.005380234993048395	2.196014282876896E-4	0.0	1.3420087284247696E-4	1.830011902397413E-4
20-21	0.005392435072397711	2.8060182503427E-4	0.0	1.4640095219179306E-4	2.074013489383735E-4
22-23	0.005429035310445659	3.2940214243153436E-4	0.0	1.4640095219179306E-4	2.8060182503427E-4
24-25	0.005538836024589504	3.4160222178085045E-4	0.0	1.5860103154110916E-4	2.928019043835861E-4
26-27	0.005648636738733349	4.026026185274309E-4	0.0	2.196014282876896E-4	3.050019837329022E-4
28-29	0.00577063753222651	4.14802697876747E-4	0.0	2.684017456849539E-4	3.4160222178085045E-4
30-31	0.005953638722466251	4.270027772260631E-4	0.0	2.928019043835861E-4	3.6600238047948265E-4
32-33	0.006161040071404625	5.368034913699078E-4	0.0	3.2940214243153436E-4	4.270027772260631E-4
34-35	0.00639284157904163	5.368034913699078E-4	0.0	3.904025391781148E-4	4.6360301527401137E-4
36-37	0.006710043642123849	5.856038087671722E-4	0.0	4.14802697876747E-4	4.880031739726435E-4
38-39	0.007466448561781446	5.856038087671722E-4	0.0	4.392028565753792E-4	4.880031739726435E-4
40-41	0.008686456496713055	6.100039674658044E-4	0.0	4.392028565753792E-4	5.002032533219596E-4
42-43	0.009747863400103553	6.344041261644366E-4	0.0	4.636030152740113E-4	5.246034120205917E-4
44-45	0.011065471969829693	6.832044435617009E-4	0.0	4.636030152740113E-4	5.368034913699078E-4
46-47	0.012468481095001042	7.564049196575975E-4	0.0	5.124033326712757E-4	5.49003570719224E-4
48-49	0.014493694266987513	8.052052370548618E-4	0.0	5.124033326712757E-4	5.6120365006854E-4
50-51	0.01659210791506988	8.174053164041778E-4	0.0	5.49003570719224E-4	5.734037294178561E-4
52-53	0.01917852473712489	8.418054751028101E-4	0.0	5.6120365006854E-4	5.856038087671722E-4
54-55	0.023216751001748515	8.906057925000745E-4	0.0	5.6120365006854E-4	5.856038087671722E-4
56-57	0.02749897885335846	9.028058718493905E-4	0.0	5.6120365006854E-4	5.978038881164884E-4
58-59	0.03290361400510549	9.394061098973387E-4	0.0	5.6120365006854E-4	6.100039674658044E-4
60-61	0.04019926145599651	9.88206427294603E-4	0.0	5.6120365006854E-4	6.344041261644366E-4
62-63	0.04978852382455895	0.0010370067446918675	0.0	5.856038087671722E-4	6.466042055137527E-4
64-65	0.06121999817486813	0.0010492068240411836	0.0	5.856038087671722E-4	6.588042848630687E-4
66-67	0.0757624927592529	0.0010614069033904995	0.0	6.344041261644366E-4	6.95404522911017E-4
68-69	0.09575842281278198	0.0010736069827398157	0.0	6.588042848630687E-4	7.076046022603331E-4
70-71	0.12046358349514705	0.0011102072207877641	0.0	6.710043642123849E-4	7.076046022603331E-4
72-73	0.1498413745683002	0.0011834076968836606	0.0	6.95404522911017E-4	7.686049990069135E-4
74-75	0.18900362927960485	0.001244408093630241	0.0	7.076046022603331E-4	7.930051577055457E-4
76-77	0.23708414199525954	0.0012688082523288732	0.0	7.076046022603331E-4	8.418054751028101E-4
78-79	0.29888974397889484	0.0013054084903768213	0.0	7.320047609589653E-4	8.540055544521262E-4
80-81	0.3764456484024972	0.0013664088871234018	0.0	7.320047609589653E-4	8.784057131507584E-4
82-83	0.47335087867411485	0.00140300912517135	0.0	7.320047609589653E-4	9.516061892466548E-4
84-85	0.5988774950992282	0.0014396093632192985	0.0	7.564049196575975E-4	9.76006347945287E-4
86-87	0.7495972753806791	0.0014640095219179306	0.0	7.808050783562296E-4	9.76006347945287E-4
88	0.8816265340989777	0.0014884096806165627	0.0	8.052052370548618E-4	0.0010248066653425513
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	2600	0.0	84.50694	1
GTGGAAT	91160	0.0	64.146255	6
AACGAGT	82965	0.0	63.91133	1
ACGAGTG	87270	0.0	63.63098	2
TGGAATC	92420	0.0	63.475117	7
GAATCAC	92915	0.0	63.24317	9
GGAATCA	93180	0.0	63.043137	8
TCGAGTG	1405	0.0	62.9166	2
CGAGTGG	90000	0.0	62.71205	3
AGTGGAA	91540	0.0	62.47844	5
ATCGAGT	1225	0.0	61.451538	1
GAGTGGA	93290	0.0	61.346725	4
CGTGTGG	1995	0.0	57.00632	3
AACGTGT	1540	0.0	54.075603	1
ACGTGTG	1995	0.0	49.966244	2
AGTACTT	38075	0.0	40.66356	20-21
CGATTGG	570	0.0	40.3991	3
GAGTACT	37400	0.0	40.310535	20-21
GTACTTT	38120	0.0	38.988697	22-23
TACTTTT	41270	0.0	37.06047	22-23
>>END_MODULE