FastQCFastQC Report
Thu 26 May 2016
SRR2132668_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132668_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4625334
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3162636.8376251315040175No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1595933.4504102838843633No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG295850.6396294840545569No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT128170.27710431289934956No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT76010.16433407836061137No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70320.1520322640483909No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT58170.12576389078064418No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT31600.085.318491
AACGAGT964700.067.736551
ACGAGTG1016550.067.220652
GTGGAAT1067200.066.973766
GAATCAC1076300.066.8135459
TGGAATC1078900.066.604627
CGAGTGG1045500.066.264833
GGAATCA1087550.066.1440058
AGTGGAA1066100.065.610325
GAGTGGA1083200.064.7090454
ATCGAGT14700.063.695881
CGTGTGG25400.056.0570453
TCGAGTG17850.055.8513372
AACGTGT20400.055.354981
ACGTGTG24850.051.283642
CGATTGG7650.043.6132283
ACGATTG7200.042.4538232
AGTACTT488950.041.4666620-21
GAGTACT481600.041.2896220-21
GTACTTT481200.040.44079222-23
Produced by FastQC (version 0.11.5)