##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132668_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4625334
Sequences flagged as poor quality	0
Sequence length	100
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.18596105708258	31.0	28.0	34.0	16.0	34.0
2	30.3060732478995	33.0	30.0	34.0	22.0	34.0
3	30.76268459747988	33.0	31.0	34.0	25.0	34.0
4	35.206640428561485	37.0	35.0	37.0	32.0	37.0
5	34.67265931498136	37.0	35.0	37.0	32.0	37.0
6	35.20289410451224	37.0	35.0	37.0	32.0	37.0
7	35.608548917764644	37.0	35.0	37.0	33.0	37.0
8	35.79997444508872	37.0	35.0	37.0	35.0	37.0
9	37.70181829031158	39.0	38.0	39.0	35.0	39.0
10-11	37.784465294830596	39.0	39.0	39.0	35.0	39.0
12-13	37.77744418024731	39.0	39.0	39.0	35.0	39.0
14-15	39.19156054460068	41.0	39.0	41.0	36.0	41.0
16-17	39.118361182133015	41.0	39.0	41.0	36.0	41.0
18-19	38.973054162142674	41.0	39.0	41.0	35.5	41.0
20-21	38.81100132444489	40.5	39.0	41.0	35.0	41.0
22-23	38.72002000287979	40.5	39.0	41.0	35.0	41.0
24-25	38.65209182731452	40.5	39.0	41.0	35.0	41.0
26-27	38.59461068108811	40.5	39.0	41.0	35.0	41.0
28-29	38.41676395693803	41.0	38.0	41.0	35.0	41.0
30-31	38.29854006218794	40.5	38.0	41.0	34.5	41.0
32-33	38.16758951894069	40.0	38.0	41.0	34.0	41.0
34-35	38.08317280438558	40.0	38.0	41.0	34.0	41.0
36-37	37.93367797871461	40.0	38.0	41.0	34.0	41.0
38-39	37.508384367485675	40.0	36.0	41.0	33.5	41.0
40-41	37.30978887146312	40.0	35.0	41.0	33.0	41.0
42-43	37.17488542449043	40.0	35.0	41.0	33.0	41.0
44-45	37.037568638286444	40.0	35.0	41.0	33.0	41.0
46-47	36.8999253891719	40.0	35.0	41.0	33.0	41.0
48-49	36.74101751354605	40.0	35.0	41.0	32.5	41.0
50-51	36.47283212412336	39.0	35.0	41.0	31.5	41.0
52-53	35.996280484825526	39.0	35.0	41.0	30.5	41.0
54-55	35.20118720075134	39.0	35.0	41.0	26.5	41.0
56-57	34.564472749427395	39.0	35.0	41.0	21.5	41.0
58-59	34.23950832523662	38.5	35.0	41.0	18.5	41.0
60-61	33.98827036058369	38.0	35.0	40.5	17.5	41.0
62-63	33.695928553483924	37.5	34.0	40.0	14.0	41.0
64-65	33.429383694237	37.0	34.0	40.0	11.5	41.0
66-67	33.119005784230936	36.5	34.0	40.0	9.0	41.0
68-69	32.826682462282726	36.0	34.0	39.0	7.0	41.0
70-71	32.49244119451698	35.5	33.5	39.0	6.0	41.0
72-73	32.153131536014484	35.0	33.0	39.0	2.0	40.5
74-75	31.802986984291294	35.0	33.0	37.5	2.0	40.0
76-77	31.439163528514914	35.0	33.0	37.0	2.0	39.0
78-79	31.315707903472486	35.0	33.0	37.0	2.0	39.0
80-81	31.175894973206262	35.0	33.0	36.0	2.0	39.0
82-83	30.868840390769616	35.0	33.0	36.0	2.0	38.0
84-85	30.600038937728606	35.0	33.0	36.0	2.0	37.0
86-87	30.412453457415182	35.0	33.0	35.0	2.0	37.0
88-89	30.20512453803336	35.0	33.0	35.0	2.0	36.0
90-91	30.012773564028024	35.0	33.0	35.0	2.0	36.0
92-93	29.851233878461535	35.0	33.0	35.0	2.0	36.0
94-95	29.677632361252183	35.0	33.0	35.0	2.0	36.0
96-97	29.50062687364848	35.0	32.5	35.0	2.0	35.5
98-99	29.35290143371268	35.0	32.0	35.0	2.0	35.0
100	29.244152314189634	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	2.880865722261511
1101	2	2.336809736806405
1101	3	2.120579003144048
1101	4	1.0791500446371955
1101	5	1.6627604653528252
1101	6	1.02020508444717
1101	7	0.5388157004771585
1101	8	0.41080767437565413
1101	9	0.4151282341923235
1101	10-11	0.3781040563305851
1101	12-13	0.28923835920979
1101	14-15	0.46197756139904556
1101	16-17	0.44054805634392125
1101	18-19	0.49137134708868757
1101	20-21	0.5231291067620631
1101	22-23	0.4724565059161776
1101	24-25	0.5136171323411034
1101	26-27	0.4302132345631975
1101	28-29	0.4801613972087466
1101	30-31	0.5032060918650174
1101	32-33	0.4805611493265971
1101	34-35	0.48262589134784406
1101	36-37	0.47680211572333775
1101	38-39	0.48026809281140004
1101	40-41	0.5087194832416557
1101	42-43	0.4737194552318442
1101	44-45	0.4334976056830726
1101	46-47	0.4286834921266518
1101	48-49	0.34834411909510976
1101	50-51	0.42973747886182423
1101	52-53	0.3721365325220063
1101	54-55	0.41866576549562495
1101	56-57	0.4222852336761207
1101	58-59	0.3641108635819208
1101	60-61	0.3709373308945487
1101	62-63	0.3408108829339582
1101	64-65	0.32923984946886975
1101	66-67	0.3401035113149504
1101	68-69	0.3163932366949851
1101	70-71	0.3679250103985936
1101	72-73	0.34585372418733584
1101	74-75	0.35421675594496094
1101	76-77	0.31272092411241914
1101	78-79	0.3635422668167294
1101	80-81	0.3509398603533356
1101	82-83	0.44643465901029344
1101	84-85	0.41851755840775695
1101	86-87	0.42702559641622884
1101	88-89	0.4173270987998592
1101	90-91	0.34510185462609755
1101	92-93	0.40536204700385525
1101	94-95	0.416727363999577
1101	96-97	0.4213963359105968
1101	98-99	0.36078661919038524
1101	100	0.38275140780721273
1102	1	2.782808375079391
1102	2	2.263543577827523
1102	3	2.0200970834357257
1102	4	0.9930438611846384
1102	5	1.5076432859763642
1102	6	0.9429209620406169
1102	7	0.45392701072088926
1102	8	0.35792148485653996
1102	9	0.3572158887192458
1102	10-11	0.2874514909189543
1102	12-13	0.2756517477589284
1102	14-15	0.4009434120142217
1102	16-17	0.3610961777948134
1102	18-19	0.4194418671945641
1102	20-21	0.48129373877932125
1102	22-23	0.3939213238283443
1102	24-25	0.44190477522344906
1102	26-27	0.3981759762780257
1102	28-29	0.47100884191918624
1102	30-31	0.426216597244526
1102	32-33	0.41217820330638943
1102	34-35	0.38625462319523507
1102	36-37	0.4266706792731796
1102	38-39	0.3255922904526045
1102	40-41	0.310299736353862
1102	42-43	0.3609847171101137
1102	44-45	0.3847197672471836
1102	46-47	0.3149064308321954
1102	48-49	0.3700076476384524
1102	50-51	0.3892362803574798
1102	52-53	0.28044823165433286
1102	54-55	0.14878430842486523
1102	56-57	0.09963273619116819
1102	58-59	0.05404222250196966
1102	60-61	0.0971518256112418
1102	62-63	0.11093859512828885
1102	64-65	0.18060031962187395
1102	66-67	0.09222207917294867
1102	68-69	0.08838178017466447
1102	70-71	0.07313060738961497
1102	72-73	0.13392800786620285
1102	74-75	0.1523523233744868
1102	76-77	0.1417177273758483
1102	78-79	0.18980414290140146
1102	80-81	0.2340634142972391
1102	82-83	0.26466451904285293
1102	84-85	0.21075657565929262
1102	86-87	0.23755583775838218
1102	88-89	0.24988513804776247
1102	90-91	0.23073143873580548
1102	92-93	0.20412108430546994
1102	94-95	0.22070968683433279
1102	96-97	0.20304115450202787
1102	98-99	0.17435931905669122
1102	100	0.1475590473166477
1103	1	2.6010094837372364
1103	2	2.157633085101775
1103	3	1.9658272841135407
1103	4	0.9812009008483642
1103	5	1.5829510772693425
1103	6	1.0157885693619235
1103	7	0.52731361239438
1103	8	0.4323381133196236
1103	9	0.4093610817070754
1103	10-11	0.29818845963262675
1103	12-13	0.2417556104879992
1103	14-15	0.38737630924150324
1103	16-17	0.4037508500342213
1103	18-19	0.40311104089806804
1103	20-21	0.4846091209210144
1103	22-23	0.4229123295594519
1103	24-25	0.5194650330359636
1103	26-27	0.4811409655747596
1103	28-29	0.5175555634966642
1103	30-31	0.5051817451262721
1103	32-33	0.5636696373476795
1103	34-35	0.5291916535137133
1103	36-37	0.4889428562272613
1103	38-39	0.5141762847337432
1103	40-41	0.44058150729275525
1103	42-43	0.47753369578834537
1103	44-45	0.45930765853854894
1103	46-47	0.481071653384447
1103	48-49	0.4417671197447177
1103	50-51	0.39202229724261883
1103	52-53	0.4219562003469477
1103	54-55	0.35594681837318376
1103	56-57	0.3872546004420627
1103	58-59	0.33259434299261414
1103	60-61	0.333707050513695
1103	62-63	0.288797706474071
1103	64-65	0.21557356105538616
1103	66-67	0.273952559293555
1103	68-69	0.31113059572755475
1103	70-71	0.2702637586958829
1103	72-73	0.341585769328546
1103	74-75	0.3818473101446429
1103	76-77	0.33019384791939643
1103	78-79	0.27228039312208807
1103	80-81	0.32755048226945505
1103	82-83	0.3977189757634392
1103	84-85	0.39774306277920246
1103	86-87	0.4057468776563127
1103	88-89	0.40379007343866036
1103	90-91	0.43745259320854757
1103	92-93	0.38829364700820435
1103	94-95	0.4134852821854871
1103	96-97	0.37361127577954534
1103	98-99	0.3971770357951172
1103	100	0.4599737221299982
1104	1	1.5961336809971947
1104	2	1.7421999587419315
1104	3	1.8160827303227869
1104	4	0.8838636316649229
1104	5	1.5569157982373198
1104	6	1.0440106859267146
1104	7	0.5229242389822133
1104	8	0.38022131793981373
1104	9	0.35726584911343195
1104	10-11	0.27296731042110167
1104	12-13	0.2731505384419677
1104	14-15	0.3731085666988534
1104	16-17	0.33658767810808143
1104	18-19	0.3576415278851144
1104	20-21	0.44210073098253844
1104	22-23	0.34116062625891885
1104	24-25	0.42506478088900224
1104	26-27	0.35584492108736043
1104	28-29	0.5021324764987796
1104	30-31	0.5146363287123705
1104	32-33	0.506294574045647
1104	34-35	0.4371092213559038
1104	36-37	0.4859535878324479
1104	38-39	0.500357171815196
1104	40-41	0.4969005226701242
1104	42-43	0.46404070754363147
1104	44-45	0.5158842105784203
1104	46-47	0.5388823308453041
1104	48-49	0.5038259154186306
1104	50-51	0.4373044473966843
1104	52-53	0.4137578734595735
1104	54-55	0.4219436749537948
1104	56-57	0.3777348098735729
1104	58-59	0.31875314866399407
1104	60-61	0.34696345649631866
1104	62-63	0.25890447398143834
1104	64-65	0.32070162844649275
1104	66-67	0.3538945344543478
1104	68-69	0.3151789539757175
1104	70-71	0.3342377426857226
1104	72-73	0.32705632118506855
1104	74-75	0.33023005929336335
1104	76-77	0.3552309953538817
1104	78-79	0.33801776820055096
1104	80-81	0.36133251637145136
1104	82-83	0.4012750402441867
1104	84-85	0.40599537642027883
1104	86-87	0.3327203034472568
1104	88-89	0.3356129419123697
1104	90-91	0.2642393303516819
1104	92-93	0.25661956574845846
1104	94-95	0.2688985628032299
1104	96-97	0.3459901146636071
1104	98-99	0.27438681246210095
1104	100	0.26277994820198813
1105	1	2.1851756320776445
1105	2	1.9443095467123577
1105	3	1.9346125589111374
1105	4	0.9401188777525178
1105	5	1.5503067639269332
1105	6	1.0418469968705395
1105	7	0.4952092646856414
1105	8	0.3468204730926914
1105	9	0.2722928686485915
1105	10-11	0.24628825969828227
1105	12-13	0.18820165540396516
1105	14-15	0.3149862622170403
1105	16-17	0.27472538013690695
1105	18-19	0.27582272143814635
1105	20-21	0.3470933280128321
1105	22-23	0.3522124759874501
1105	24-25	0.41002277921182895
1105	26-27	0.31752550398521606
1105	28-29	0.4123297928841154
1105	30-31	0.4653164854755829
1105	32-33	0.5065022470089175
1105	34-35	0.44627732758460326
1105	36-37	0.4326519499653969
1105	38-39	0.42313770577991505
1105	40-41	0.36045575347041137
1105	42-43	0.3579132673961567
1105	44-45	0.4294802780516136
1105	46-47	0.42207021172959713
1105	48-49	0.41244927017616106
1105	50-51	0.41529388921936317
1105	52-53	0.3671719266103466
1105	54-55	0.2981354762965083
1105	56-57	0.21108825720833835
1105	58-59	0.18526416314696093
1105	60-61	0.27176040762056886
1105	62-63	0.3173908287642675
1105	64-65	0.23235392541459277
1105	66-67	0.23916954978347604
1105	68-69	0.20022619546510612
1105	70-71	0.18948973650662282
1105	72-73	0.21203263867103317
1105	74-75	0.2115820666887558
1105	76-77	0.20327890198344178
1105	78-79	0.17298855843766603
1105	80-81	0.16770996732240917
1105	82-83	0.21068853072791782
1105	84-85	0.23004122216859102
1105	86-87	0.17039257470159086
1105	88-89	0.20266077382808945
1105	90-91	0.22790549987766795
1105	92-93	0.23753896133222696
1105	94-95	0.29798399950166043
1105	96-97	0.3009242744494287
1105	98-99	0.2975940618500914
1105	100	0.2363696802569102
1106	1	1.4380065169755376
1106	2	0.9625774279569583
1106	3	1.4420504301629755
1106	4	0.7297338170496275
1106	5	1.4349797147575174
1106	6	0.9714216721488924
1106	7	0.5225263343068534
1106	8	0.36394835429324957
1106	9	0.2620621083514365
1106	10-11	0.16935698417602651
1106	12-13	0.14647279767600452
1106	14-15	0.22668796992883244
1106	16-17	0.21000392367741227
1106	18-19	0.16839587027609326
1106	20-21	0.23901937449769406
1106	22-23	0.20015901964782046
1106	24-25	0.31061296198734567
1106	26-27	0.238415697455288
1106	28-29	0.3573945016832738
1106	30-31	0.2879901239367868
1106	32-33	0.2902624548338295
1106	34-35	0.3083228841187733
1106	36-37	0.2895517373284022
1106	38-39	0.2807853464993286
1106	40-41	0.26436538643631735
1106	42-43	0.29012988234052983
1106	44-45	0.24602961889992514
1106	46-47	0.27299811573628574
1106	48-49	0.23721691718402127
1106	50-51	0.12394995432349276
1106	52-53	0.2151240808464152
1106	54-55	0.2205636568586158
1106	56-57	0.22224947307945087
1106	58-59	0.21468145507527225
1106	60-61	0.1655464939864828
1106	62-63	0.17307802327631805
1106	64-65	0.13635938816214122
1106	66-67	0.15289423385588918
1106	68-69	0.18340271193142854
1106	70-71	0.15118754266620726
1106	72-73	0.1424405418849659
1106	74-75	0.19740302172937163
1106	76-77	0.014769686517134062
1106	78-79	0.07624483699260054
1106	80-81	0.055892409839618296
1106	82-83	0.11312146208807405
1106	84-85	0.07639227512333235
1106	86-87	0.1073728460600556
1106	88-89	0.0491143777660632
1106	90-91	0.0905301903744764
1106	92-93	0.11148577441513652
1106	94-95	0.10794437026305559
1106	96-97	0.12647731588410593
1106	98-99	0.14406483261776515
1106	100	0.18125081025366185
1107	1	1.5512635554975311
1107	2	1.1135076229244802
1107	3	1.351002729864181
1107	4	0.7174392180706732
1107	5	1.4109701300403046
1107	6	1.0024745302460545
1107	7	0.5554651305199911
1107	8	0.42934660342039876
1107	9	0.37183339100312196
1107	10-11	0.17401981431800095
1107	12-13	0.13632690778221956
1107	14-15	0.24176913041642223
1107	16-17	0.23456961478959926
1107	18-19	0.2629939285921097
1107	20-21	0.20391051112759584
1107	22-23	0.25599926304908394
1107	24-25	0.344318537059074
1107	26-27	0.31790734719017877
1107	28-29	0.2758472810982511
1107	30-31	0.20337325014127572
1107	32-33	0.17797267021989427
1107	34-35	0.16111482867654559
1107	36-37	0.27178061753896543
1107	38-39	0.273443023066406
1107	40-41	0.29089625170274047
1107	42-43	0.30570355662945303
1107	44-45	0.2771926201843371
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2214	4	-1.381270322430339
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2214	100	-0.47703915160549926
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2215	4	-1.7702313549792734
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2215	8	-1.079273066137766
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2215	28-29	-0.8167672152788512
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2216	1	-5.788034805604884
2216	2	-4.818541557264691
2216	3	-5.779035076198131
2216	4	-2.9781201368096504
2216	5	-4.180999744425748
2216	6	-1.8577607810198984
2216	7	-1.0919841398565708
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2216	10-11	-0.8342208632515025
2216	12-13	-0.5641678931414447
2216	14-15	-1.4329567607763707
2216	16-17	-0.9544981010384816
2216	18-19	-1.5101677988015112
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2216	32-33	-1.0469047014050332
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2216	36-37	-1.1257567329231293
2216	38-39	-1.0889743879571654
2216	40-41	-1.1607287151971306
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2216	54-55	-0.9484442527349444
2216	56-57	-0.9219259585676269
2216	58-59	-0.8226605398083748
2216	60-61	-0.8080193512289853
2216	62-63	-0.8632829548585264
2216	64-65	-0.8790444150018928
2216	66-67	-0.9406125481405496
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2216	70-71	-0.8829658818434787
2216	72-73	-0.8869610203014666
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2216	76-77	-0.9029070448774661
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2216	80-81	-0.8573745927622625
2216	82-83	-0.8304223099771448
2216	84-85	-0.8430645368463274
2216	86-87	-0.9049728217832254
2216	88-89	-0.8556905712261802
2216	90-91	-0.9129151938938556
2216	92-93	-0.8882340188795084
2216	94-95	-0.9713278315675318
2216	96-97	-0.9763267414970578
2216	98-99	-1.0057081417919704
2216	100	-1.0520809768145334
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	7939.0
3	6936.0
4	7780.0
5	10029.0
6	8390.0
7	6082.0
8	5797.0
9	6057.0
10	6565.0
11	12091.0
12	8317.0
13	10993.0
14	10340.0
15	10708.0
16	13011.0
17	16445.0
18	22507.0
19	37558.0
20	77960.0
21	104017.0
22	47731.0
23	29729.0
24	29742.0
25	32481.0
26	33448.0
27	36540.0
28	42461.0
29	49811.0
30	58524.0
31	72610.0
32	94213.0
33	128553.0
34	186859.0
35	403404.0
36	524492.0
37	894685.0
38	1148550.0
39	418995.0
40	2984.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	14.8110875698576	42.35407295173428	25.761073054273574	17.073766424134547
2	17.554377479595328	40.2489622965212	24.790587911538726	17.406072312344744
3	18.74647728066282	25.72312090826758	21.202538282108552	34.32786352896105
4	32.26305386810985	26.86677329680408	22.486311258819363	18.383861576266707
5	17.426201005159843	39.400419515650114	23.715973808594146	19.457405670595897
6	35.054398233727554	24.465887220252636	23.682959976511967	16.796754569507847
7	18.743489659341357	24.49933345354087	38.61651936919582	18.140657517921948
8	34.07779848979555	24.710972223843726	23.412860563150684	17.798368723210043
9	33.766534481617974	26.12447014637213	23.294425872812642	16.814569499197248
10-11	16.89235847616626	42.48553726066053	23.246267188488442	17.375837074684767
12-13	16.886077848648334	25.950623241478343	31.221334502546195	25.941964407327124
14-15	17.134016700199382	33.95733151378906	23.065880215353097	25.842771570658464
16-17	25.71478421158968	25.59690071810019	31.116864214754862	17.571450855555263
18-19	17.493536682972515	25.794039954736242	31.20746523386203	25.504958128429212
20-21	25.856081308968893	34.23776084681708	22.819858950299654	17.086298893914368
22-23	25.451114148494614	26.032651536345764	31.243235611933084	17.27299870322654
24-25	17.316284618581058	34.04865464850755	22.910821142862332	25.724239590049063
26-27	17.658071311256812	29.26971963883746	35.80713436876281	17.26507468114292
28-29	24.836005267549073	25.8313891355395	32.1335087165138	17.199096880397622
30-31	22.889370923374713	26.995490340962224	32.429880828895115	17.685257906767948
32-33	19.5644698458959	28.063831776761745	33.342503944353666	19.02919443298869
34-35	19.44982584163929	27.897206299039972	33.66962546520514	18.9833423941156
36-37	19.435208404414446	27.937212080956574	33.892447088625765	18.73513242600321
38-39	20.105217761802997	27.827050290613226	33.41749932618555	18.650232621398228
40-41	19.049706990700606	28.903735832082305	33.34073842732452	18.705818749892572
42-43	19.12867676112805	27.98121691156876	33.83993747907438	19.05016884822881
44-45	19.091991780365518	28.41637341329413	33.22384775017829	19.26778705616206
46-47	19.605952824430684	27.98278486727047	33.56909803246669	18.842164275832157
48-49	19.15296982271082	28.178525241214754	33.52312400145854	19.14538093461589
50-51	19.66583825522453	28.731959672348644	32.760194995973976	18.84200707645285
52-53	19.813409492782867	28.429734573046815	32.663868018058	19.09298791611232
54-55	19.796522456925285	29.371192255080075	31.07582529651738	19.756459991477268
56-57	20.23684937363556	30.062358083606423	30.056282805286788	19.644509737471225
58-59	21.17144897166074	30.315358093182116	28.89627099662238	19.616921938534766
60-61	20.699961135492266	30.860224527666503	28.626720727431156	19.813093609410075
62-63	20.05041239379091	30.90280604200658	28.55111770678849	20.495663857414023
64-65	20.39511145307103	30.807441383177654	28.954111845181384	19.84333531856993
66-67	20.304955150919685	30.16069298769861	29.58486815987739	19.949483701504313
68-69	20.661045606310918	30.202271034082596	29.41002218577868	19.726661173827807
70-71	19.61760637108656	31.021705578549476	29.50265454732584	19.858033503038126
72-73	19.68421709226284	30.21267790949873	29.812322417518434	20.29078258072
74-75	19.89054639778736	30.656035163523683	29.151164343097314	20.30225409559164
76-77	20.33662599540381	30.27200515098701	29.348258741964827	20.043110111644356
78-79	20.13066394874467	30.608549496536085	28.891464720874392	20.369321833844854
80-81	20.631184336317407	31.088183729066355	28.41410608417557	19.86652585044067
82-83	20.52141304346651	31.0329592017758	28.54550307546929	19.900124679288396
84-85	20.19397937053026	31.40319701214949	27.977294048536137	20.425529568784107
86-87	20.269177505370255	31.43514129143365	28.220697662602173	20.074983540593923
88-89	21.159424958706865	31.008028148626533	27.877468547868634	19.955078344797972
90-91	20.685828557887397	31.526667398911318	27.733160823411996	20.054343219789285
92-93	20.17584335076451	31.626137468093596	27.676446072363348	20.521573108778547
94-95	20.59499560914979	31.78711765738006	27.42465689151589	20.193229841954256
96-97	20.592970885957847	31.366702743399294	27.689373385966228	20.35095298467663
98-99	21.0663389579711	31.427863512556954	27.0611119927459	20.444685536726045
100	20.36987590014501	32.177793652073724	26.982740522789477	20.46958992499179
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	157600.0
1	85254.5
2	9395.0
3	4919.5
4	3699.0
5	3331.5
6	3247.5
7	3461.0
8	3369.0
9	3178.0
10	3417.5
11	5131.0
12	41894.0
13	46740.5
14	14529.0
15	13339.5
16	14802.5
17	16596.5
18	18507.5
19	20564.5
20	23068.0
21	25993.5
22	29305.5
23	33358.5
24	37761.5
25	42318.0
26	48886.5
27	56028.0
28	61797.0
29	67058.0
30	72457.5
31	78673.0
32	85424.5
33	92146.0
34	98840.5
35	104089.5
36	109702.5
37	115456.0
38	120313.5
39	124911.0
40	127497.0
41	129664.0
42	131062.0
43	131204.5
44	132860.5
45	134925.0
46	136462.0
47	140344.5
48	142312.5
49	150883.5
50	185222.0
51	180987.5
52	136663.0
53	118133.0
54	113296.0
55	109724.0
56	101741.0
57	90878.5
58	81029.0
59	69228.0
60	56216.5
61	46066.0
62	37628.5
63	29266.0
64	22547.0
65	17208.0
66	12787.5
67	9693.5
68	7378.5
69	5494.5
70	4047.0
71	3090.0
72	2393.0
73	1681.5
74	1162.5
75	879.0
76	652.0
77	468.0
78	308.5
79	190.0
80	115.5
81	69.5
82	40.0
83	22.5
84	15.0
85	7.0
86	3.0
87	5.5
88	6.5
89	5.0
90	2.5
91	0.0
92	0.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05872008378205769
2	0.06719514742070519
3	2.1620060302672198E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	9.729027136202488E-5
18-19	0.0
20-21	3.0268084423741077E-4
22-23	1.7296048242137758E-4
24-25	0.0
26-27	0.0022376762413265725
28-29	7.134619899881824E-4
30-31	0.005653645769148779
32-33	0.0011134331055876181
34-35	0.0019349953970891616
36-37	0.007264340261697858
38-39	0.011772122834805011
40-41	0.0030159984122227713
42-43	0.006972469447611783
44-45	0.0014593540704303733
46-47	0.0021836260905698916
48-49	0.0032538190755521655
50-51	0.009037185206516979
52-53	0.0027133175679853604
54-55	0.0012323434372523152
56-57	6.810318995341742E-4
58-59	7.891322010475352E-4
60-61	0.006302247578228945
62-63	0.0017836549749704561
64-65	0.007794031739113326
66-67	0.010593829548309376
68-69	0.011339721628751567
70-71	0.009512826533175766
72-73	0.004205101728869742
74-75	0.001870135216181145
76-77	0.0043456321208371115
78-79	0.01562049356868066
80-81	0.00850749372910151
82-83	0.005783366130964812
84-85	0.004388872241442456
86-87	0.00689679923655243
88-89	0.003686220281605609
90-91	0.006183337246564248
92-93	0.010356008884979982
94-95	0.008118332643653409
96-97	0.010150618312104596
98-99	0.0019025653066351534
100	0.0023133464523859247
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4625334.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.61314796724747
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.14466349404418	45.59773208700377
2	14.270621513066095	16.44350857633724
3	3.7610579353995313	6.500591620366904
4	1.141776629995129	2.6312538371781815
5	0.4783162108616148	1.3778651315751678
6	0.2590888149073831	0.8956153337950714
7	0.1648194882115418	0.6647038693552291
8	0.1200108796813584	0.5531363655009307
9	0.09538171058007984	0.4945716544517382
>10	0.4609358752034178	5.035740575567612
>50	0.08415727316065871	3.1524053224851816
>100	0.01475061017370169	1.5094550457117357
>500	0.0014731884129979163	0.5814156401174743
>1k	0.0026819579163028127	2.8381984692236903
>5k	1.1332216547757761E-4	0.44504971587334835
>10k+	1.5109622063677017E-4	11.278756755456714
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	316263	6.8376251315040175	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	159593	3.4504102838843633	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	29585	0.6396294840545569	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	12817	0.27710431289934956	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7601	0.16433407836061137	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7032	0.1520322640483909	No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5817	0.12576389078064418	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.004129431517810389	0.0	0.0	4.3240120605344396E-5	2.1620060302672198E-5
2	0.004129431517810389	0.0	0.0	4.3240120605344396E-5	2.1620060302672198E-5
3	0.004129431517810389	0.0	0.0	6.48601809080166E-5	2.1620060302672198E-5
4	0.004129431517810389	0.0	2.1620060302672198E-5	8.648024121068879E-5	6.48601809080166E-5
5	0.004129431517810389	0.0	2.1620060302672198E-5	8.648024121068879E-5	8.648024121068879E-5
6	0.004129431517810389	0.0	2.1620060302672198E-5	1.0810030151336098E-4	1.0810030151336098E-4
7	0.004129431517810389	0.0	2.1620060302672198E-5	1.297203618160332E-4	1.5134042211870538E-4
8	0.004129431517810389	0.0	2.1620060302672198E-5	1.297203618160332E-4	1.7296048242137758E-4
9	0.004129431517810389	0.0	2.1620060302672198E-5	1.5134042211870538E-4	1.7296048242137758E-4
10-11	0.004129431517810389	0.0	2.1620060302672198E-5	1.8377051257271367E-4	1.8377051257271367E-4
12-13	0.004129431517810389	0.0	2.1620060302672198E-5	2.3782066332939415E-4	2.594407236320664E-4
14-15	0.004161861608264398	0.0	2.1620060302672198E-5	2.9187081408607466E-4	3.891610854480995E-4
16-17	0.004259151879626423	0.0	2.1620060302672198E-5	3.0268084423741077E-4	4.756413266587883E-4
18-19	0.004291581970080431	0.0	2.1620060302672198E-5	3.2430090454008294E-4	6.161717186261576E-4
20-21	0.004324012060534439	0.0	2.1620060302672198E-5	3.6754102514542734E-4	7.134619899881824E-4
22-23	0.004356442150988448	0.0	2.1620060302672198E-5	3.999711155994356E-4	7.67512140744863E-4
24-25	0.004399682271593792	0.0	2.1620060302672198E-5	4.215911759021078E-4	7.78322170896199E-4
26-27	0.004475352482653145	0.0	2.1620060302672198E-5	4.6483129650745224E-4	8.215622915015435E-4
28-29	0.004507782573107153	0.0	2.1620060302672198E-5	5.080714171127966E-4	8.8642247240956E-4
30-31	0.004626692904771849	0.0	2.1620060302672198E-5	5.729315980208133E-4	9.512826533175766E-4
32-33	0.004713173145982539	0.0	2.1620060302672198E-5	6.053616884748215E-4	0.0010593829548309376
34-35	0.0050266640203712855	0.0	2.1620060302672198E-5	6.377917789288298E-4	0.0011350531658902902
36-37	0.005437445166122058	0.0	2.1620060302672198E-5	7.134619899881824E-4	0.0011674832563442986
38-39	0.005923896522932182	0.0	2.1620060302672198E-5	7.567021105935269E-4	0.0011674832563442986
40-41	0.0066697886033743724	0.0	2.1620060302672198E-5	7.78322170896199E-4	0.0011782932864956347
42-43	0.007404870653665227	0.0	2.1620060302672198E-5	8.539923819555517E-4	0.0012431534674036513
44-45	0.008410203457739483	0.0	2.1620060302672198E-5	9.188525628635684E-4	0.0012647735277063234
46-47	0.00950201650302443	0.0	2.1620060302672198E-5	9.296625930149045E-4	0.0012863935880089957
48-49	0.010810030151336098	0.0	2.1620060302672198E-5	9.620926834689127E-4	0.0013080136483116678
50-51	0.012334244402674489	0.0	2.1620060302672198E-5	0.001005332804074257	0.0013728738292196844
52-53	0.014247619739460978	0.0	2.1620060302672198E-5	0.0010377628945282655	0.001426923979976365
54-55	0.01714470782001905	0.0	2.1620060302672198E-5	0.0010701929849822737	0.0014485440402790372
56-57	0.019987745749820443	0.0	2.1620060302672198E-5	0.001102623075436282	0.001535024281489726
58-59	0.023868546574150103	0.0	2.1620060302672198E-5	0.0011134331055876181	0.001535024281489726
60-61	0.029014120926186087	0.0	2.1620060302672198E-5	0.0011782932864956347	0.0015998844623977427
62-63	0.035662289469257785	0.0	2.1620060302672198E-5	0.0011891033166469707	0.0016323145528517509
64-65	0.04387791238427322	0.0	2.1620060302672198E-5	0.001199913346798307	0.0017187947940624396
66-67	0.0548609030180307	0.0	2.1620060302672198E-5	0.0012215334071009791	0.0017836549749704563
68-69	0.06980036468717718	1.0810030151336099E-5	2.1620060302672198E-5	0.0012863935880089955	0.001870135216181145
70-71	0.09050157242698581	2.1620060302672198E-5	2.1620060302672198E-5	0.0013404437387656762	0.0019349953970891616
72-73	0.11608891379519835	2.1620060302672198E-5	2.1620060302672198E-5	0.0013620637990683483	0.0020539057287538584
74-75	0.15037832943523646	2.1620060302672198E-5	2.1620060302672198E-5	0.0013944938895223567	0.0021728160604185557
76-77	0.19153211422137298	2.1620060302672198E-5	2.1620060302672198E-5	0.0014053039196736928	0.002205246150872564
78-79	0.24335539876687823	2.1620060302672198E-5	2.1620060302672198E-5	0.001426923979976365	0.0023349665126885973
80-81	0.3103343455845567	2.1620060302672198E-5	2.1620060302672198E-5	0.0014485440402790372	0.0023998266935966136
82-83	0.39477149109664295	2.1620060302672198E-5	2.1620060302672198E-5	0.0014701641005817093	0.0024863069348073025
84-85	0.502169140650167	4.3240120605344396E-5	2.1620060302672198E-5	0.0015242142513383899	0.002518737025261311
86-87	0.6321381331596809	4.3240120605344396E-5	2.1620060302672198E-5	0.001535024281489726	0.0025619771458666556
88	0.7467352627939949	4.3240120605344396E-5	2.1620060302672198E-5	0.001556644341792398	0.0026160272966233356
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	3160	0.0	85.31849	1
AACGAGT	96470	0.0	67.73655	1
ACGAGTG	101655	0.0	67.22065	2
GTGGAAT	106720	0.0	66.97376	6
GAATCAC	107630	0.0	66.813545	9
TGGAATC	107890	0.0	66.60462	7
CGAGTGG	104550	0.0	66.26483	3
GGAATCA	108755	0.0	66.144005	8
AGTGGAA	106610	0.0	65.61032	5
GAGTGGA	108320	0.0	64.709045	4
ATCGAGT	1470	0.0	63.69588	1
CGTGTGG	2540	0.0	56.057045	3
TCGAGTG	1785	0.0	55.851337	2
AACGTGT	2040	0.0	55.35498	1
ACGTGTG	2485	0.0	51.28364	2
CGATTGG	765	0.0	43.613228	3
ACGATTG	720	0.0	42.453823	2
AGTACTT	48895	0.0	41.46666	20-21
GAGTACT	48160	0.0	41.28962	20-21
GTACTTT	48120	0.0	40.440792	22-23
>>END_MODULE