FastQCFastQC Report
Thu 26 May 2016
SRR2132667_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132667_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5950492
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3201095.379538364222656No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA268650.45147527296902507No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT127630.21448646599306412No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT74490.12518292605048456No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGAGT743100.088.81161
ACGAGTG799500.086.661792
CGAGTGG826150.085.0100563
TACGAGT35750.082.397181
GTGGAAT883650.082.280796
GAATCAC889600.082.274529
GAGTGGA866000.082.1834264
TGGAATC891800.081.94517
GGAATCA897300.081.610398
AGTGGAA878600.080.8550955
ATCGAGT14050.057.9407161
AACGTGT22050.054.8451961
CGTGTGG28750.053.4458663
ACGTGTG25450.053.2145232
TCGAGTG17100.052.7995952
TGAGTAC893150.041.5854418-19
ATCACTG914100.041.3815110-11
GAGTACT488400.041.24879520-21
GCTGAGT917600.041.10983316-17
AGTACTT502100.041.00307520-21
Produced by FastQC (version 0.11.5)