##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2132667_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5950492 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.164071307044864 31.0 28.0 34.0 16.0 34.0 2 30.290637816167134 33.0 30.0 34.0 19.0 34.0 3 30.751608606481614 33.0 31.0 34.0 25.0 34.0 4 35.21532807707329 37.0 35.0 37.0 32.0 37.0 5 34.69880255279732 37.0 35.0 37.0 32.0 37.0 6 35.265725086261774 37.0 35.0 37.0 32.0 37.0 7 35.70111597494795 37.0 35.0 37.0 35.0 37.0 8 35.90243411805276 37.0 35.0 37.0 35.0 37.0 9 37.83023420584382 39.0 39.0 39.0 35.0 39.0 10-11 37.93429854203652 39.0 39.0 39.0 35.0 39.0 12-13 37.95343460675184 39.0 39.0 39.0 35.0 39.0 14-15 39.401132208899696 41.0 39.0 41.0 37.0 41.0 16-17 39.38079708366972 41.0 39.0 41.0 36.5 41.0 18-19 39.395551914026605 41.0 39.5 41.0 37.0 41.0 20-21 39.301709841808034 41.0 39.0 41.0 36.0 41.0 22-23 39.24459901803078 40.5 39.0 41.0 36.0 41.0 24-25 39.20753863714126 41.0 39.0 41.0 36.0 41.0 26-27 39.15403264133453 41.0 39.0 41.0 36.0 41.0 28-29 38.99573934390635 41.0 39.0 41.0 35.5 41.0 30-31 38.90675653374545 41.0 39.0 41.0 35.0 41.0 32-33 38.778446303263664 41.0 39.0 41.0 35.0 41.0 34-35 38.70036645709296 40.5 39.0 41.0 35.0 41.0 36-37 38.600236249372315 40.0 38.5 41.0 35.0 41.0 38-39 38.25605168446576 40.0 38.0 41.0 34.5 41.0 40-41 38.076706346298764 40.0 37.0 41.0 34.0 41.0 42-43 37.95265046991072 40.0 37.0 41.0 34.0 41.0 44-45 37.83313026889205 40.0 37.0 41.0 33.0 41.0 46-47 37.70751452148831 40.0 37.0 41.0 33.0 41.0 48-49 37.57562752794223 40.0 36.0 41.0 33.0 41.0 50-51 37.35999359380703 40.0 36.0 41.0 33.0 41.0 52-53 36.980760750539616 40.0 36.0 41.0 32.5 41.0 54-55 36.31817688352493 40.0 35.0 41.0 30.5 41.0 56-57 35.741794880154444 39.0 35.0 41.0 28.0 41.0 58-59 35.426621949916075 39.0 35.0 41.0 27.0 41.0 60-61 35.18411284310608 39.0 35.0 41.0 26.0 41.0 62-63 34.880075294614294 38.5 35.0 40.5 26.0 41.0 64-65 34.616033598566304 38.0 35.0 40.0 26.0 41.0 66-67 34.32696976989466 37.0 34.5 40.0 26.0 41.0 68-69 34.01498245859334 37.0 34.0 40.0 25.0 41.0 70-71 33.66128817583487 36.0 34.0 39.0 24.5 41.0 72-73 33.30250507016899 36.0 34.0 39.0 24.0 41.0 74-75 32.93452869107294 35.0 34.0 38.5 23.0 40.0 76-77 32.5532123226113 35.0 33.5 37.0 21.5 39.5 78-79 32.43729014340327 35.0 34.0 37.0 22.5 39.0 80-81 32.29604686469623 35.0 34.0 37.0 22.5 39.0 82-83 31.99333130773052 35.0 34.0 36.0 20.0 38.5 84-85 31.72653093223216 35.0 34.0 36.0 20.0 37.0 86-87 31.51559047554387 35.0 34.0 36.0 18.5 37.0 88-89 31.302955537121974 35.0 34.0 35.0 16.5 36.5 90-91 31.118354919223485 35.0 34.0 35.0 9.0 36.0 92-93 30.955334533682258 35.0 33.0 35.0 3.5 36.0 94-95 30.802402473610584 35.0 33.0 35.0 2.0 36.0 96-97 30.657770819622982 35.0 33.0 35.0 2.0 36.0 98-99 30.51663080968767 35.0 33.0 35.0 2.0 35.5 100 30.406675952173366 35.0 33.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 2.861728349410267 1101 2 2.3123256751994887 1101 3 2.1073023698949385 1101 4 1.0582407983732196 1101 5 1.6605739764409364 1101 6 1.106066142828631 1101 7 0.6069205603746255 1101 8 0.481543058537099 1101 9 0.42837559063274 1101 10-11 0.36085292518649936 1101 12-13 0.3168273657560903 1101 14-15 0.4098430823307453 1101 16-17 0.3942023869664766 1101 18-19 0.37785143257827514 1101 20-21 0.39200372415191964 1101 22-23 0.37296112582362895 1101 24-25 0.4150220755425167 1101 26-27 0.36786230253534313 1101 28-29 0.37667344634976985 1101 30-31 0.3785031984507867 1101 32-33 0.40142253987469445 1101 34-35 0.3997832077405761 1101 36-37 0.4004649700859062 1101 38-39 0.3671390887001351 1101 40-41 0.361148251732466 1101 42-43 0.36777701463494594 1101 44-45 0.3812424353557944 1101 46-47 0.42034014253643903 1101 48-49 0.33117987398927795 1101 50-51 0.4153199647173196 1101 52-53 0.3999129275233244 1101 54-55 0.3293421269967567 1101 56-57 0.3384921315681453 1101 58-59 0.2841544450969451 1101 60-61 0.2582810815677945 1101 62-63 0.2495840380934382 1101 64-65 0.23110497574758426 1101 66-67 0.2608439990028799 1101 68-69 0.22247061223880849 1101 70-71 0.23122667324148694 1101 72-73 0.2382595406971859 1101 74-75 0.30276139334404917 1101 76-77 0.15006052766355538 1101 78-79 0.22983391940868358 1101 80-81 0.2558262838849714 1101 82-83 0.25384739810143486 1101 84-85 0.27239451433775486 1101 86-87 0.30696848178374125 1101 88-89 0.24798130038982436 1101 90-91 0.22475661170246752 1101 92-93 0.2632122839053288 1101 94-95 0.2940815783389432 1101 96-97 0.31550896431607 1101 98-99 0.28628909631412114 1101 100 0.27433446643251 1102 1 2.824203751053492 1102 2 2.2896926047187307 1102 3 2.1035205246194195 1102 4 1.0680532509865586 1102 5 1.6145753720444915 1102 6 1.0495214057497293 1102 7 0.5417233196413562 1102 8 0.47611051380120983 1102 9 0.4447850310386272 1102 10-11 0.37945830338492925 1102 12-13 0.31701212034657544 1102 14-15 0.40851477851344953 1102 16-17 0.35412457005114817 1102 18-19 0.40318246384110523 1102 20-21 0.402536771809622 1102 22-23 0.33577583184210624 1102 24-25 0.3876161615880491 1102 26-27 0.3016590046522438 1102 28-29 0.4152349149791874 1102 30-31 0.3986623053995828 1102 32-33 0.3595218697625242 1102 34-35 0.3971823914733861 1102 36-37 0.39542274145839684 1102 38-39 0.3279855040787041 1102 40-41 0.34856430645697145 1102 42-43 0.4064726233900231 1102 44-45 0.40719897797742277 1102 46-47 0.3577257665088851 1102 48-49 0.35577861562206436 1102 50-51 0.3555471247197062 1102 52-53 0.3766727814159836 1102 54-55 0.38509858444405864 1102 56-57 0.32487607082502734 1102 58-59 0.2706180242340821 1102 60-61 0.2689935082792303 1102 62-63 0.2882375115790552 1102 64-65 0.3048702757914228 1102 66-67 0.28604706021338444 1102 68-69 0.24802150667846234 1102 70-71 0.21412800957260458 1102 72-73 0.25413934796644355 1102 74-75 0.34201414606578595 1102 76-77 0.2342323578250003 1102 78-79 0.28980960211922735 1102 80-81 0.3348942840025586 1102 82-83 0.385037794199782 1102 84-85 0.31617202889217566 1102 86-87 0.28392243848666254 1102 88-89 0.3137372741629676 1102 90-91 0.29394603886342097 1102 92-93 0.3542342735004773 1102 94-95 0.3375620851778187 1102 96-97 0.33696211069096904 1102 98-99 0.3210039152759734 1102 100 0.27896954470727664 1103 1 2.539503118389 1103 2 2.164560730927491 1103 3 2.0169101828811478 1103 4 1.0028445324196724 1103 5 1.6226961013112913 1103 6 1.0494105385922268 1103 7 0.5628115254248698 1103 8 0.4216680720509203 1103 9 0.41870672856561697 1103 10-11 0.3103072798474642 1103 12-13 0.19783351617666511 1103 14-15 0.35712226430202776 1103 16-17 0.3575714164233119 1103 18-19 0.3318432640448066 1103 20-21 0.35366985272061413 1103 22-23 0.3004836675137028 1103 24-25 0.37687967444330894 1103 26-27 0.34925136661701117 1103 28-29 0.38890917322734 1103 30-31 0.3389515366756086 1103 32-33 0.30631160552746195 1103 34-35 0.2739312479199967 1103 36-37 0.2978720251984299 1103 38-39 0.3339779233979101 1103 40-41 0.28961449624752333 1103 42-43 0.33426488278405486 1103 44-45 0.2613427793359122 1103 46-47 0.2821548519355517 1103 48-49 0.28569653509330806 1103 50-51 0.2540104905303622 1103 52-53 0.28415018199034847 1103 54-55 0.2898918372830437 1103 56-57 0.21544048442373764 1103 58-59 0.2538691765796699 1103 60-61 0.32128101926429053 1103 62-63 0.2523364571040432 1103 64-65 0.22785828355268478 1103 66-67 0.2606177806012866 1103 68-69 0.30934982588717475 1103 70-71 0.318873007905637 1103 72-73 0.269220279281825 1103 74-75 0.20744278067257227 1103 76-77 0.26123880487415363 1103 78-79 0.24781096388395696 1103 80-81 0.28431246527367193 1103 82-83 0.36269168762013493 1103 84-85 0.40421615199377214 1103 86-87 0.431992066529606 1103 88-89 0.4343300665301868 1103 90-91 0.36177548534164927 1103 92-93 0.35772498316482526 1103 94-95 0.37881880855227834 1103 96-97 0.3399957055034122 1103 98-99 0.3378451298407654 1103 100 0.43427689682271264 1104 1 1.6467431652702942 1104 2 1.806005123485086 1104 3 1.8546496254332467 1104 4 0.9020554707780946 1104 5 1.5748606986082905 1104 6 1.0438983238172952 1104 7 0.5411875280619398 1104 8 0.4080443402265814 1104 9 0.36772904296330466 1104 10-11 0.3055208323874794 1104 12-13 0.2572266002699948 1104 14-15 0.3145819527977949 1104 16-17 0.29748186582448 1104 18-19 0.32923160049135447 1104 20-21 0.3546477672355053 1104 22-23 0.2496686242950048 1104 24-25 0.311628832045038 1104 26-27 0.2538960027375907 1104 28-29 0.32928591754115644 1104 30-31 0.3152494709685385 1104 32-33 0.27322904591862596 1104 34-35 0.2519867729538774 1104 36-37 0.29159970622249887 1104 38-39 0.26042888574637146 1104 40-41 0.23583194487954273 1104 42-43 0.2230825035583308 1104 44-45 0.26382865543011036 1104 46-47 0.27970823434773706 1104 48-49 0.23845493160651188 1104 50-51 0.20617749140939168 1104 52-53 0.19450366878667324 1104 54-55 0.17574046197629656 1104 56-57 0.1404827187935851 1104 58-59 0.138543937594946 1104 60-61 0.08241006186034383 1104 62-63 0.07180959192439929 1104 64-65 0.14255698882316636 1104 66-67 0.10266455659660068 1104 68-69 0.11088205219930103 1104 70-71 0.11064497435620524 1104 72-73 0.007295422123270612 1104 74-75 0.11680118451097599 1104 76-77 0.0973586909672548 1104 78-79 0.10498428275001714 1104 80-81 0.14641850673431378 1104 82-83 0.15596600729197263 1104 84-85 0.08699666146159046 1104 86-87 0.07387620014129581 1104 88-89 0.1166763288456849 1104 90-91 0.11720552050261901 1104 92-93 0.10696625243926405 1104 94-95 0.1023527379739555 1104 96-97 0.05830298858585792 1104 98-99 0.06895057155046658 1104 100 0.10807464798558541 1105 1 2.157721642390932 1105 2 2.014082985666313 1105 3 1.9966633259215811 1105 4 0.9598247328507981 1105 5 1.5316838130955404 1105 6 1.0249640680748442 1105 7 0.5544494199049552 1105 8 0.42334434926980435 1105 9 0.3457257421855857 1105 10-11 0.2861270676922345 1105 12-13 0.20352679017774733 1105 14-15 0.29589299457749973 1105 16-17 0.24560200509016283 1105 18-19 0.23251372655393254 1105 20-21 0.3382360065192813 1105 22-23 0.30044464617961353 1105 24-25 0.3670395075740629 1105 26-27 0.24378906658277089 1105 28-29 0.28869765564930816 1105 30-31 0.31110767317508703 1105 32-33 0.2900398390096015 1105 34-35 0.2870400149506196 1105 36-37 0.29045686845798 1105 38-39 0.26421573689486166 1105 40-41 0.17527793420853044 1105 42-43 0.24917108196309812 1105 44-45 0.25046333168260304 1105 46-47 0.374087303799719 1105 48-49 0.2645412492046333 1105 50-51 0.2523629453793603 1105 52-53 0.16286876645352066 1105 54-55 0.12533182117326191 1105 56-57 0.19185050682035865 1105 58-59 0.07251481840521734 1105 60-61 0.13640829843115654 1105 62-63 0.1646315307921853 1105 64-65 0.144084163719171 1105 66-67 0.10436468320177283 1105 68-69 0.019624591537336755 1105 70-71 0.08316769546309644 1105 72-73 0.14589191335134188 1105 74-75 0.18570265855692014 1105 76-77 0.09445468481786179 1105 78-79 0.09083615469792505 1105 80-81 0.1862465723881428 1105 82-83 0.25643738547970685 1105 84-85 0.25808998756034285 1105 86-87 0.20158182050663953 1105 88-89 0.21273461244127034 1105 90-91 0.2255852003723966 1105 92-93 0.283332910062704 1105 94-95 0.29576135616892785 1105 96-97 0.3147683304198239 1105 98-99 0.3338481570089584 1105 100 0.3125747836339876 1106 1 1.676040269986192 1106 2 1.114107088705314 1106 3 1.5789689359226458 1106 4 0.8055700979270171 1106 5 1.48594327180664 1106 6 0.9634946870421572 1106 7 0.5259462420193017 1106 8 0.4142581190296184 1106 9 0.3183451395519228 1106 10-11 0.3245643064037509 1106 12-13 0.210774161577028 1106 14-15 0.3386092153935891 1106 16-17 0.3230859853015602 1106 18-19 0.29864958243733497 1106 20-21 0.34285922853123196 1106 22-23 0.27008448050659695 1106 24-25 0.3674424153185427 1106 26-27 0.3085788098664395 1106 28-29 0.31709928191062886 1106 30-31 0.24887361738194613 1106 32-33 0.2782271015221056 1106 34-35 0.2562052206274643 1106 36-37 0.24496516441831773 1106 38-39 0.27638966227132045 1106 40-41 0.29029132612317454 1106 42-43 0.3084914135090031 1106 44-45 0.33041689114708817 1106 46-47 0.2833181102588327 1106 48-49 0.31887575142066993 1106 50-51 0.24259953397880452 1106 52-53 0.33098195044566836 1106 54-55 0.40513894894130686 1106 56-57 0.3475738702937292 1106 58-59 0.4207616487863248 1106 60-61 0.4133230331301192 1106 62-63 0.4094621522667765 1106 64-65 0.3407575582519229 1106 66-67 0.3710934992808461 1106 68-69 0.3995042078382127 1106 70-71 0.33220971151368417 1106 72-73 0.2752466021180382 1106 74-75 0.3416102695848764 1106 76-77 0.37781442180063607 1106 78-79 0.40847868330543946 1106 80-81 0.37738151983701584 1106 82-83 0.43907676113808947 1106 84-85 0.3883945979200618 1106 86-87 0.37292723724553056 1106 88-89 0.33568832960999373 1106 90-91 0.37306650206459224 1106 92-93 0.3541387439474555 1106 94-95 0.3857815113785996 1106 96-97 0.3780411640578478 1106 98-99 0.40591589729080013 1106 100 0.42115175229335833 1107 1 1.7260710196064508 1107 2 1.1290905875395403 1107 3 1.4576104976453195 1107 4 0.758856137666136 1107 5 1.443860089830821 1107 6 0.9558248277743431 1107 7 0.44996499222708763 1107 8 0.37534780195661455 1107 9 0.3630985293764937 1107 10-11 0.15992357326965134 1107 12-13 0.14877687283626528 1107 14-15 0.23856968731547568 1107 16-17 0.21422462967416322 1107 18-19 0.19904004312791557 1107 20-21 0.21872436250917815 1107 22-23 0.21382875352185948 1107 24-25 0.2826444527213354 1107 26-27 0.22301230690494833 1107 28-29 0.26794501599707843 1107 30-31 0.22818668387462537 1107 32-33 0.24596740304962594 1107 34-35 0.17113282420186948 1107 36-37 0.23111818400212059 1107 38-39 0.26541411597494147 1107 40-41 0.21504911240403857 1107 42-43 0.21643783589547638 1107 44-45 0.23191401571568093 1107 46-47 0.2455115438834028 1107 48-49 0.23829236035187762 1107 50-51 0.22229089696586612 1107 52-53 0.1773918335993372 1107 54-55 0.1779721848165181 1107 56-57 0.10939995964808702 1107 58-59 0.20509853386479904 1107 60-61 0.17800368906716102 1107 62-63 0.21660869536122362 1107 64-65 0.2067965012477586 1107 66-67 0.19331930367723515 1107 68-69 0.2132173709773184 1107 70-71 0.16281720303516067 1107 72-73 0.18593857476038522 1107 74-75 0.13477464734788214 1107 76-77 0.18210916138620803 1107 78-79 0.14601813216060577 1107 80-81 0.210307280324443 1107 82-83 0.23207114592720046 1107 84-85 0.1940463736758815 1107 86-87 0.21954903759771582 1107 88-89 0.1747703019487865 1107 90-91 0.19497580386085644 1107 92-93 0.2798146045893546 1107 94-95 0.2733975868864569 1107 96-97 0.26924818477823464 1107 98-99 0.2581107007773227 1107 100 0.22243072745871828 1108 1 0.5389060535715622 1108 2 0.21078508302762344 1108 3 -0.008637349624748936 1108 4 0.049069773799679695 1108 5 1.0767587325305996 1108 6 0.6842307609643967 1108 7 0.2831954277775992 1108 8 0.21672575247593073 1108 9 0.2257417269488613 1108 10-11 0.0994991547305446 1108 12-13 0.0992813261771488 1108 14-15 0.1044965730803682 1108 16-17 0.09210170583864397 1108 18-19 0.06288512217131625 1108 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-0.5786931003992919 2214 42-43 -0.5730765670766615 2214 44-45 -0.5875041136072738 2214 46-47 -0.6609643253257147 2214 48-49 -0.602424086045481 2214 50-51 -0.5735851494956563 2214 52-53 -0.4975534433696822 2214 54-55 -0.541464820104089 2214 56-57 -0.5661170940088383 2214 58-59 -0.6448454874314109 2214 60-61 -0.6077487181791241 2214 62-63 -0.5172836148985169 2214 64-65 -0.5226967416123145 2214 66-67 -0.5638986276544742 2214 68-69 -0.5775100929362651 2214 70-71 -0.5924921056318979 2214 72-73 -0.5286602823343287 2214 74-75 -0.558245108030853 2214 76-77 -0.5533581676276143 2214 78-79 -0.5054439570521154 2214 80-81 -0.5125701870726189 2214 82-83 -0.4713278388410842 2214 84-85 -0.4899096113467749 2214 86-87 -0.5424495236442866 2214 88-89 -0.5278402013086705 2214 90-91 -0.53884951017886 2214 92-93 -0.5773575554433101 2214 94-95 -0.5980143078184916 2214 96-97 -0.6431839481318988 2214 98-99 -0.6248686138359574 2214 100 -0.6812557658764966 2215 1 -5.464013316149266 2215 2 -4.339353249416675 2215 3 -3.6641013498448487 2215 4 -1.9135842280560311 2215 5 -2.7899456947575914 2215 6 -5.015944740004905 2215 7 -2.1622064397037235 2215 8 -1.2240117967804878 2215 9 -1.6620594331507448 2215 10-11 -0.9241260194148353 2215 12-13 -0.7034986025878496 2215 14-15 -0.8818844056809922 2215 16-17 -0.7455669092262411 2215 18-19 -0.7620743526061275 2215 20-21 -0.7459836556673665 2215 22-23 -0.8368359650850508 2215 24-25 -0.9069684672705947 2215 26-27 -0.8713166078868113 2215 28-29 -0.889535974386888 2215 30-31 -1.027807942259578 2215 32-33 -0.9056137587582498 2215 34-35 -0.8974353051129569 2215 36-37 -0.883732097317484 2215 38-39 -0.8844573828953699 2215 40-41 -0.9345290752056101 2215 42-43 -0.9167397729079312 2215 44-45 -0.9018311759303757 2215 46-47 -0.9112618051092269 2215 48-49 -0.957848764418813 2215 50-51 -0.9334506350535321 2215 52-53 -0.9316099594707552 2215 54-55 -0.9817301682205439 2215 56-57 -0.9622406059572981 2215 58-59 -0.8658854968552276 2215 60-61 -0.8336910828374826 2215 62-63 -0.7479194479561144 2215 64-65 -0.7649837220709728 2215 66-67 -0.7839800548526128 2215 68-69 -0.7364238813926178 2215 70-71 -0.7344084151531476 2215 72-73 -0.701411930358951 2215 74-75 -0.7366343022363822 2215 76-77 -0.7391190782652259 2215 78-79 -0.7974681933417429 2215 80-81 -0.8022775464545617 2215 82-83 -0.7472667609373715 2215 84-85 -0.7446493014444044 2215 86-87 -0.7362892627535516 2215 88-89 -0.6990641022829678 2215 90-91 -0.7291899909889459 2215 92-93 -0.7905559142385492 2215 94-95 -0.8117622528862931 2215 96-97 -0.8081595663083654 2215 98-99 -0.7489390241828282 2215 100 -0.7690481144478163 2216 1 -5.895880703522732 2216 2 -4.937366387143701 2216 3 -5.933656607058488 2216 4 -3.10588321845556 2216 5 -4.15888421058434 2216 6 -1.9529684860236287 2216 7 -1.275279917841651 2216 8 -0.8481044201329979 2216 9 -0.7461024500594604 2216 10-11 -1.086058950916076 2216 12-13 -0.9219489207920688 2216 14-15 -1.8117505115139139 2216 16-17 -1.3398593942293004 2216 18-19 -1.870522318753551 2216 20-21 -1.1849531495609966 2216 22-23 -0.8927054227138669 2216 24-25 -1.6488473903001761 2216 26-27 -1.3604742360666506 2216 28-29 -2.1107391454735662 2216 30-31 -2.1703197820588755 2216 32-33 -1.3149897392357701 2216 34-35 -1.2513616799018905 2216 36-37 -1.26216702125312 2216 38-39 -1.2439463949874678 2216 40-41 -1.2453465055899855 2216 42-43 -1.6294926529006588 2216 44-45 -1.4126906974082019 2216 46-47 -1.8825452963815863 2216 48-49 -1.4678429059408344 2216 50-51 -1.3031454725684455 2216 52-53 -1.1196624489450855 2216 54-55 -1.099161281180308 2216 56-57 -1.0139456256487875 2216 58-59 -1.044506606527392 2216 60-61 -1.0020064403764977 2216 62-63 -0.9324211171491257 2216 64-65 -0.9847998344127689 2216 66-67 -0.9818775552337229 2216 68-69 -0.9880965842062679 2216 70-71 -1.0002524114101874 2216 72-73 -0.9810088991224646 2216 74-75 -0.9902804017555233 2216 76-77 -0.9262382790122672 2216 78-79 -0.9169722303251646 2216 80-81 -0.8649033761675895 2216 82-83 -0.8307947979529402 2216 84-85 -0.8235711001673067 2216 86-87 -0.8709244838466468 2216 88-89 -0.9273790318672219 2216 90-91 -0.9427358036186355 2216 92-93 -0.9795514109829213 2216 94-95 -1.0060315598305039 2216 96-97 -0.9996206864005543 2216 98-99 -1.021588027597705 2216 100 -1.072322309661704 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7156.0 3 2084.0 4 2271.0 5 2837.0 6 2764.0 7 2861.0 8 3240.0 9 4004.0 10 4937.0 11 10232.0 12 7610.0 13 10683.0 14 10177.0 15 10453.0 16 12526.0 17 15931.0 18 21937.0 19 36967.0 20 76900.0 21 106227.0 22 51836.0 23 35258.0 24 35715.0 25 38388.0 26 40008.0 27 43237.0 28 49480.0 29 59221.0 30 70525.0 31 87802.0 32 114380.0 33 154903.0 34 225154.0 35 378448.0 36 655380.0 37 1222786.0 38 1692091.0 39 640753.0 40 3330.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 15.519068179590128 38.28611795361514 27.625533114160206 18.569280752634526 2 19.178625613250208 35.518180659382494 26.50860307597929 18.794590651388006 3 20.4402761990101 24.962792992579438 21.79090401264299 32.80602679576747 4 30.185638431242324 25.787531518402172 23.918375152844504 20.108454897510995 5 18.95685264344528 34.494828326800544 24.91755303595064 21.63076599380354 6 33.33563006218645 22.99315754058656 25.12116645144637 18.550045945780617 7 20.377004119995455 23.218651499741533 36.38623495334504 20.018109426917977 8 32.14181281144484 23.22357546233152 25.0100663945099 19.624545331713747 9 32.05284537816369 24.971514960443606 24.48315198138238 18.492487680010324 10-11 18.558230142986496 37.83981223737466 24.633223605711933 18.968734013926916 12-13 18.5341565033614 24.72914004421819 30.960675184505753 25.776028267914654 14-15 18.720141124465002 31.087320174533467 24.53481997791107 25.65771872309046 16-17 25.438394900718603 24.496211275533735 31.014687793122093 19.05070603062557 18-19 18.9078146815423 24.57784163057441 31.091353454470656 25.422990233412634 20-21 25.563665085560395 31.23679202080506 24.413286334284237 18.786256559350303 22-23 25.24196342954172 24.75290253602815 31.097913683016813 18.90722035141331 24-25 18.838543098621088 31.12737568590967 24.46456528300517 25.56951593246407 26-27 19.098760591623435 27.355794350540364 34.678503191162726 18.86694186667348 28-29 24.924898328836044 24.551043213910383 31.67148242224705 18.852576035006525 30-31 23.72752929253799 25.273450941852733 31.83090389543382 19.168115870175455 32-33 21.048163067754995 26.366258795612403 32.638288790184205 19.947289346448397 34-35 20.473098817421015 26.16061176310439 33.076978342003 20.28931107747159 36-37 20.436107091725546 26.384795856996913 32.935906460917074 20.24319059036046 38-39 20.817478416023167 26.33474593363389 32.71900290834087 20.128772742002063 40-41 20.526626520845646 26.604816086831136 32.69101364164679 20.177543750676435 42-43 20.695400453494997 26.425600425411016 32.725127123725244 20.153871997368743 44-45 20.48492468054184 26.523152775919822 32.67456676774691 20.317355775791427 46-47 20.59723452893678 26.526128537979115 32.631499783415826 20.245137149668274 48-49 20.486599853670988 26.677533925030033 32.54780292622737 20.28806329507161 50-51 20.658644592629543 26.731352173711358 32.24949730382748 20.360505929831614 52-53 20.755900372108446 26.842116629272077 31.94473236266217 20.45725063595731 54-55 21.106599649317666 27.26000161837873 30.986734588156644 20.64666414414696 56-57 21.404988003861554 27.794791878572678 29.935980541997072 20.8642395755687 58-59 21.571890026752694 28.26556707087842 29.31181936630115 20.850723536067736 60-61 21.538338722580384 28.434468548533477 29.082228593709036 20.944964135177106 62-63 21.295815857215903 28.525367907021103 29.07407212897658 21.104744106786413 64-65 21.22572974829879 28.46010382130782 29.36915790858905 20.94500852180434 66-67 21.1641976401113 28.270276325421573 29.561719949420272 21.003806085046854 68-69 21.137593060554593 28.227977454533843 29.683894546918317 20.950534937993247 70-71 20.980775968149224 28.283934919255806 29.767160478534787 20.968128634060186 72-73 20.972879612362057 28.27961083269337 29.722177849233926 21.02533170571065 74-75 21.077213866904724 28.28415347014614 29.53966384269614 21.098968820253 76-77 21.146215499000874 28.34892667292977 29.414287610983852 21.090570217085506 78-79 21.15491508461788 28.650800040541146 29.066153504044284 21.128131370796694 80-81 21.25392913791111 28.784134287124864 28.836756688620678 21.125179886343343 82-83 21.267795571014556 28.931344742487326 28.702070360109687 21.098789326388438 84-85 21.252137012489104 29.02209384158062 28.494060061132526 21.23170908479775 86-87 21.29749135447497 29.130324845524235 28.388691612086536 21.183492187914258 88-89 21.38793435078403 29.154616014067354 28.288271497358675 21.169178137789945 90-91 21.391574550815477 29.268269727850683 28.08547766127585 21.254678060057987 92-93 21.392244255748917 29.353583773831595 27.972242411988375 21.281929558431116 94-95 21.377439447483365 29.478313095168968 27.840951633365624 21.303295823982047 96-97 21.320878678717946 29.460652186956963 27.80105775037367 21.417411383951418 98-99 21.491357946679187 29.57106354916591 27.53208145710434 21.405497047050563 100 21.473749275875996 29.763273842145253 27.439136190436336 21.32384069154242 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 24219.0 1 13750.5 2 2699.5 3 1944.5 4 1787.5 5 1758.5 6 1702.5 7 1870.0 8 1915.5 9 1913.0 10 2083.0 11 3062.0 12 25060.0 13 30588.0 14 14947.0 15 15813.5 16 17966.5 17 20169.0 18 22615.5 19 25345.0 20 28301.5 21 32070.5 22 36750.5 23 41955.0 24 47807.0 25 54352.0 26 62151.5 27 70765.5 28 79553.5 29 88941.0 30 98431.5 31 108070.0 32 117732.5 33 127164.5 34 136188.0 35 143862.5 36 152718.5 37 161165.0 38 167473.0 39 173041.0 40 178093.5 41 184578.5 42 188677.5 43 191673.5 44 195321.0 45 196923.5 46 198046.0 47 198738.5 48 197340.0 49 195180.0 50 194962.5 51 187923.5 52 175256.0 53 166044.5 54 157707.5 55 149078.5 56 138195.5 57 126424.0 58 114552.5 59 98976.0 60 81976.5 61 66735.0 62 53883.0 63 42506.5 64 31947.5 65 24261.0 66 18239.5 67 13427.5 68 10194.0 69 7294.0 70 5115.5 71 3792.0 72 2856.5 73 2067.5 74 1506.0 75 1130.5 76 823.0 77 569.5 78 389.5 79 222.0 80 114.0 81 66.5 82 36.5 83 18.5 84 7.0 85 6.0 86 5.5 87 3.5 88 2.5 89 2.0 90 2.0 91 2.0 92 1.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06041517239246772 2 0.06550718831316805 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 1.0923466496551882E-4 18-19 0.0 20-21 2.856906622175107E-4 22-23 1.0083199842970969E-4 24-25 0.0 26-27 0.002571215959957597 28-29 9.831119846896693E-4 30-31 0.0064616505660372294 32-33 0.001378037311872699 34-35 0.0025123972942069327 36-37 0.008545511866917896 38-39 0.01189817581470574 40-41 0.003092181285177763 42-43 0.0080917678739842 44-45 0.0017477546394483012 46-47 0.0025123972942069327 48-49 0.0034030799470027018 50-51 0.009679871849252128 52-53 0.0035375226115756477 54-55 0.001554493309124691 56-57 5.965893240424489E-4 58-59 0.0010587359835119517 60-61 0.006251583902642 62-63 0.0018401839713422016 64-65 0.0077472585460160275 66-67 0.011385613156021384 68-69 0.012385530473782672 70-71 0.01044451450401076 72-73 0.005050002588021293 74-75 0.0020670559678090485 76-77 0.005075210587628721 78-79 0.016486031743257534 80-81 0.009318557188212336 82-83 0.006991018557793205 84-85 0.005117223920307766 86-87 0.007537191882620798 88-89 0.004386191931692371 90-91 0.006318805234928473 92-93 0.011688109151310515 94-95 0.009301751855140719 96-97 0.011679706484774705 98-99 0.0028569066221751076 100 0.0033442612812520376 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5950492.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.2346524013502 #Duplication Level Percentage of deduplicated Percentage of total 1 87.66902776335637 64.20410774613725 2 8.969434975775693 13.137469053748918 3 1.6387655764181355 3.6004328206884137 4 0.5181095895535803 1.5177430278705073 5 0.25388930954988925 0.9296747666652472 6 0.16475662761111542 0.7239536612351242 7 0.11476873040062281 0.588353365420372 8 0.08710495854604666 0.5103281089242998 9 0.07501708802138748 0.4944465328867025 >10 0.4961208514206586 6.144577833163921 >50 0.007515216345208181 0.36430805410019046 >100 0.004612600067225992 0.7593388450082861 >500 3.922169007732865E-4 0.19794063694393635 >1k 3.691398349193049E-4 0.5240507459434237 >5k 4.614247934519147E-5 0.22510730224958986 >10k+ 6.92137190177872E-5 6.078167499013788 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 320109 5.379538364222656 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 26865 0.45147527296902507 No Hit TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 12763 0.21448646599306412 No Hit ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 7449 0.12518292605048456 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0013612319788010806 3.361066614323656E-5 0.0 0.0 0.0 2 0.0013612319788010806 3.361066614323656E-5 0.0 0.0 0.0 3 0.001378037311872699 3.361066614323656E-5 0.0 0.0 0.0 4 0.001378037311872699 6.722133228647312E-5 0.0 1.680533307161828E-5 0.0 5 0.001378037311872699 6.722133228647312E-5 0.0 3.361066614323656E-5 0.0 6 0.001378037311872699 1.512479976445645E-4 0.0 5.041599921485484E-5 0.0 7 0.001378037311872699 1.512479976445645E-4 0.0 5.041599921485484E-5 0.0 8 0.001378037311872699 1.512479976445645E-4 0.0 5.041599921485484E-5 0.0 9 0.001378037311872699 1.680533307161828E-4 0.0 8.40266653580914E-5 0.0 10-11 0.001378037311872699 1.9326133032361023E-4 0.0 1.0923466496551881E-4 0.0 12-13 0.001386439978408508 2.2687199646684677E-4 0.0 1.4284533110875538E-4 0.0 14-15 0.0013948426449443172 2.6888532914589246E-4 0.0 1.512479976445645E-4 0.0 16-17 0.0014032453114801264 2.8569066221751076E-4 0.0 1.7645599725199193E-4 0.0 18-19 0.0014200506445517446 3.6971732757560215E-4 0.0 2.1846932993103765E-4 0.0 20-21 0.001445258644159172 3.781199941114113E-4 0.0 2.6048266261008337E-4 0.0 22-23 0.001445258644159172 3.8652266064722045E-4 0.0 2.772879956817016E-4 0.0 24-25 0.0014620639772307902 3.9492532718302955E-4 0.0 3.1089866182493816E-4 0.0 26-27 0.0014872719768382176 4.20133326790457E-4 0.0 3.2770399489655646E-4 0.0 28-29 0.0015292853095172634 4.20133326790457E-4 0.0 3.361066614323656E-4 0.0 30-31 0.0016049093083395456 4.2853599332626615E-4 0.0 3.61314661039793E-4 0.0 32-33 0.0017057413067692554 4.369386598620753E-4 0.0 4.033279937188387E-4 0.0 34-35 0.0018149759717347742 4.369386598620753E-4 0.0 4.1173066025464785E-4 0.0 36-37 0.0019410159697719111 4.621466594695027E-4 0.0 4.20133326790457E-4 0.0 38-39 0.002226706631989422 4.957573256127392E-4 0.0 4.20133326790457E-4 0.0 40-41 0.002630034625708261 5.041599921485484E-4 0.0 4.621466594695027E-4 0.0 42-43 0.0030921812851777636 5.797839909708307E-4 0.0 4.873546590769301E-4 0.0 44-45 0.0038820319395438223 6.133946571140671E-4 0.0 4.873546590769301E-4 0.0 46-47 0.004839935924626065 6.554079897931129E-4 0.0 4.873546590769301E-4 0.0 48-49 0.005949087907352871 7.142266555437769E-4 0.0 5.041599921485484E-4 0.0 50-51 0.007453165217262707 7.22629322079586E-4 0.0 5.293679917559757E-4 0.0 52-53 0.009150503857496153 7.310319886153951E-4 0.0 5.545759913634032E-4 0.0 54-55 0.011200754492233584 7.982533209018683E-4 0.0 5.545759913634032E-4 0.0 56-57 0.01357870912186757 8.570719866525323E-4 0.0 5.713813244350215E-4 0.0 58-59 0.017267479731087783 8.738773197241506E-4 0.0 5.713813244350215E-4 0.0 60-61 0.02216623432146451 9.242933189390054E-4 0.0 5.881866575066397E-4 0.0 62-63 0.028090114229209956 9.663066516180511E-4 0.0 6.217973236498763E-4 0.0 64-65 0.03624910343548063 0.001016722650832906 0.0 6.301999901856855E-4 0.0 66-67 0.046139041948127985 0.0010503333169761425 0.0 6.638106563289221E-4 0.0 68-69 0.059070745746738255 0.0010755413165835699 0.0 6.722133228647312E-4 0.0 70-71 0.07660711080697193 0.0010755413165835699 0.0 6.722133228647312E-4 0.0 72-73 0.09777342781067516 0.0011175546492626157 0.0 6.974213224721586E-4 0.0 74-75 0.1258635420398851 0.001151165315405852 0.0 7.22629322079586E-4 0.0 76-77 0.16312936812619866 0.0011595679819416612 0.0 7.22629322079586E-4 0.0 78-79 0.21089012471573781 0.0011847759815490889 0.0 7.478373216870135E-4 0.0 80-81 0.27163300110310207 0.0012267893142281344 0.0 7.562399882228226E-4 0.0 82-83 0.346567981269448 0.001260399980371371 0.0 7.562399882228226E-4 0.0 84-85 0.4385687771700223 0.0013108159795862258 0.0 7.8144798783025E-4 0.0 86-87 0.5546600180287613 0.0013276213126578442 0.0 7.898506543660591E-4 0.0 88 0.6555256271246143 0.0013444266457294624 0.0 8.066559874376774E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACGAGT 74310 0.0 88.8116 1 ACGAGTG 79950 0.0 86.66179 2 CGAGTGG 82615 0.0 85.010056 3 TACGAGT 3575 0.0 82.39718 1 GTGGAAT 88365 0.0 82.28079 6 GAATCAC 88960 0.0 82.27452 9 GAGTGGA 86600 0.0 82.183426 4 TGGAATC 89180 0.0 81.9451 7 GGAATCA 89730 0.0 81.61039 8 AGTGGAA 87860 0.0 80.855095 5 ATCGAGT 1405 0.0 57.940716 1 AACGTGT 2205 0.0 54.845196 1 CGTGTGG 2875 0.0 53.445866 3 ACGTGTG 2545 0.0 53.214523 2 TCGAGTG 1710 0.0 52.799595 2 TGAGTAC 89315 0.0 41.58544 18-19 ATCACTG 91410 0.0 41.38151 10-11 GAGTACT 48840 0.0 41.248795 20-21 GCTGAGT 91760 0.0 41.109833 16-17 AGTACTT 50210 0.0 41.003075 20-21 >>END_MODULE