FastQCFastQC Report
Thu 26 May 2016
SRR2132666_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132666_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5301010
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3446576.501723256511495No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2209284.167658615999593No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT137390.2591770247556598No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG127320.24018064482051535No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC95770.1806636848449635No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA91740.17306136000498018No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT91610.17281612371981941No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT63430.11965644282881942No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGAGT850700.080.7505951
TACGAGT36050.080.2618941
ACGAGTG912250.078.862552
CGAGTGG941250.077.375973
GTGGAAT986200.076.579646
TGGAATC999850.075.933677
GAATCAC1003050.075.7851269
GGAATCA1005150.075.631478
GAGTGGA977700.075.486244
AGTGGAA991650.074.3200765
AACGTGT22600.055.99941
CGTGTGG30150.052.8381733
ATCGAGT16000.049.9883161
TCGAGTG18650.047.9004552
ACGTGTG31800.046.426652
ATCTGCG28200.045.83582394
AGTACTT512650.041.6679620-21
ACGATTG7500.041.3759082
GAGTACT504200.041.35967320-21
GTACTTT507750.040.0020822-23
Produced by FastQC (version 0.11.5)