##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132666_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5301010
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.220048443598483	31.0	28.0	34.0	16.0	34.0
2	30.33643324573996	33.0	30.0	34.0	23.0	34.0
3	30.79135560959138	33.0	31.0	34.0	25.0	34.0
4	35.23032233480035	37.0	35.0	37.0	32.0	37.0
5	34.690173947983496	37.0	35.0	37.0	32.0	37.0
6	35.20602790788925	37.0	35.0	37.0	32.0	37.0
7	35.60818749634504	37.0	35.0	37.0	33.0	37.0
8	35.784239984455795	37.0	35.0	37.0	35.0	37.0
9	37.6764908951313	39.0	38.0	39.0	35.0	39.0
10-11	37.74556905193539	39.0	39.0	39.0	35.0	39.0
12-13	37.721129086721206	39.0	39.0	39.0	35.0	39.0
14-15	39.123365641641875	41.0	39.0	41.0	36.0	41.0
16-17	39.02319029769799	41.0	39.0	41.0	36.0	41.0
18-19	38.83405539699039	41.0	39.0	41.0	35.5	41.0
20-21	38.65425749055369	40.5	39.0	41.0	35.0	41.0
22-23	38.55712222765096	40.0	39.0	41.0	35.0	41.0
24-25	38.49252812199939	40.5	38.5	41.0	35.0	41.0
26-27	38.416681538046525	40.0	38.0	41.0	35.0	41.0
28-29	38.23359897453504	40.0	38.0	41.0	34.5	41.0
30-31	38.1175722173699	40.0	38.0	41.0	34.0	41.0
32-33	37.9741171022126	40.0	38.0	41.0	34.0	41.0
34-35	37.88980920617014	40.0	38.0	41.0	34.0	41.0
36-37	37.754528570970436	40.0	38.0	41.0	34.0	41.0
38-39	37.340685548603005	40.0	36.0	41.0	33.0	41.0
40-41	37.13979232259513	40.0	35.0	41.0	33.0	41.0
42-43	37.00208215038266	40.0	35.0	41.0	33.0	41.0
44-45	36.86137622830366	40.0	35.0	41.0	33.0	41.0
46-47	36.712211069211335	40.0	35.0	41.0	33.0	41.0
48-49	36.54069705584408	39.0	35.0	41.0	32.0	41.0
50-51	36.273230856006684	39.0	35.0	41.0	31.0	41.0
52-53	35.802424726608706	39.0	35.0	41.0	30.5	41.0
54-55	35.03502964529401	39.0	35.0	41.0	26.0	41.0
56-57	34.40379984191692	38.0	35.0	41.0	22.0	41.0
58-59	34.06016240301376	38.0	35.0	40.0	20.0	41.0
60-61	33.798688174517686	37.0	34.5	40.0	18.5	41.0
62-63	33.48722526461938	37.0	34.0	40.0	18.0	41.0
64-65	33.20754742964077	36.0	34.0	40.0	16.0	41.0
66-67	32.90869664460169	35.5	34.0	39.0	14.0	41.0
68-69	32.613621272172665	35.0	33.5	39.0	12.5	41.0
70-71	32.28599842294204	35.0	33.0	38.5	9.0	40.5
72-73	31.958542994636872	35.0	33.0	37.0	7.0	40.0
74-75	31.62984450133088	35.0	33.0	37.0	4.0	39.0
76-77	31.289789964553925	35.0	33.0	36.5	2.0	39.0
78-79	31.210865948187234	35.0	33.0	36.0	2.0	39.0
80-81	31.102330216317267	35.0	33.0	36.0	2.0	38.0
82-83	30.845060375286973	35.0	33.0	35.5	2.0	37.0
84-85	30.62299816450073	35.0	33.0	35.0	2.0	37.0
86-87	30.457844448510755	35.0	33.0	35.0	2.0	36.0
88-89	30.276748487552375	35.0	33.0	35.0	2.0	36.0
90-91	30.119486946827113	35.0	33.0	35.0	2.0	36.0
92-93	29.980140199697793	35.0	33.0	35.0	2.0	36.0
94-95	29.830809977721227	35.0	33.0	35.0	2.0	35.0
96-97	29.681405147320984	35.0	33.0	35.0	2.0	35.0
98-99	29.543524158603738	35.0	33.0	35.0	2.0	35.0
100	29.433537948428697	35.0	33.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	2.736692892169092
1101	2	2.2114869957529493
1101	3	2.0144954487761595
1101	4	0.9824876210241129
1101	5	1.5859059007341472
1101	6	1.0224658464579335
1101	7	0.5060169625340833
1101	8	0.4163304091567568
1101	9	0.3691753121288528
1101	10-11	0.31843761193791664
1101	12-13	0.301638004083145
1101	14-15	0.4469212664410378
1101	16-17	0.42452136623261794
1101	18-19	0.43799799512774484
1101	20-21	0.5150763964061014
1101	22-23	0.43943891482457786
1101	24-25	0.5053275269811977
1101	26-27	0.4992184147632841
1101	28-29	0.5460325445174519
1101	30-31	0.5287485309047497
1101	32-33	0.4765649063606716
1101	34-35	0.4833207617900044
1101	36-37	0.4687477263720936
1101	38-39	0.44362397886088445
1101	40-41	0.48013862948059227
1101	42-43	0.4882646672124906
1101	44-45	0.5264176447305005
1101	46-47	0.5969278099524828
1101	48-49	0.5227350143029028
1101	50-51	0.46392059511904904
1101	52-53	0.4238104381917722
1101	54-55	0.5167789807634833
1101	56-57	0.48403852180700824
1101	58-59	0.43081738781700807
1101	60-61	0.44026653053450815
1101	62-63	0.4295611078646431
1101	64-65	0.42650803961601014
1101	66-67	0.40905894305439716
1101	68-69	0.48548798870625376
1101	70-71	0.47424081503893945
1101	72-73	0.4200100708634231
1101	74-75	0.42356132810277103
1101	76-77	0.39096605406816565
1101	78-79	0.45750902463613485
1101	80-81	0.447806746965405
1101	82-83	0.4981761020029971
1101	84-85	0.557182663328117
1101	86-87	0.47668398720828975
1101	88-89	0.44055421605428435
1101	90-91	0.3818528093859328
1101	92-93	0.49354661983814907
1101	94-95	0.5205608651091218
1101	96-97	0.5128895346094389
1101	98-99	0.4448082227656549
1101	100	0.45730262659582976
1102	1	2.6819448634170584
1102	2	2.1724307276179644
1102	3	1.9852731232311882
1102	4	0.9518764616844209
1102	5	1.4914831453784245
1102	6	0.8566053133481617
1102	7	0.37010494901448254
1102	8	0.3055555896388782
1102	9	0.31853698479781656
1102	10-11	0.24242079406320727
1102	12-13	0.23587949953622456
1102	14-15	0.36791250971117506
1102	16-17	0.35160304674105447
1102	18-19	0.38915205309257317
1102	20-21	0.4333266520853982
1102	22-23	0.36081645823603026
1102	24-25	0.4584535242385286
1102	26-27	0.3956783673882356
1102	28-29	0.5030782795808051
1102	30-31	0.48837115506431417
1102	32-33	0.5021593607263029
1102	34-35	0.5095308378046965
1102	36-37	0.5287754181817874
1102	38-39	0.4659075230056757
1102	40-41	0.4300164602928618
1102	42-43	0.4552159328440837
1102	44-45	0.47099538261586815
1102	46-47	0.44682559544683187
1102	48-49	0.4989567125372574
1102	50-51	0.5250995271449739
1102	52-53	0.41198345895303845
1102	54-55	0.448679408679844
1102	56-57	0.39780819169465076
1102	58-59	0.3611440562610895
1102	60-61	0.3770593837069498
1102	62-63	0.339440909988447
1102	64-65	0.4158375451340319
1102	66-67	0.3686470088703899
1102	68-69	0.350746651987933
1102	70-71	0.3744033338143993
1102	72-73	0.3839885243202552
1102	74-75	0.4075301741970563
1102	76-77	0.3206438298159391
1102	78-79	0.3644077981383518
1102	80-81	0.44277477719989733
1102	82-83	0.4683020628346064
1102	84-85	0.4224195299815108
1102	86-87	0.4222542067110666
1102	88-89	0.42237487450920597
1102	90-91	0.4071172788507553
1102	92-93	0.4560287582045177
1102	94-95	0.48404502856740095
1102	96-97	0.4236469231817068
1102	98-99	0.41172077415261654
1102	100	0.4119230000528873
1103	1	2.569606919163313
1103	2	2.189658282434479
1103	3	1.996699082954855
1103	4	1.0013385116237217
1103	5	1.551099778038278
1103	6	0.9959157151968157
1103	7	0.49233888898753264
1103	8	0.39452009457366444
1103	9	0.38360319715137337
1103	10-11	0.25544298840124213
1103	12-13	0.23098761634869902
1103	14-15	0.40807435299271333
1103	16-17	0.44010118851595337
1103	18-19	0.434742175078469
1103	20-21	0.5321022836788103
1103	22-23	0.48762394258903186
1103	24-25	0.6056452454170298
1103	26-27	0.563867412259043
1103	28-29	0.5728584047717433
1103	30-31	0.5804468349950156
1103	32-33	0.6058010261530669
1103	34-35	0.5542099550281421
1103	36-37	0.5251418666017145
1103	38-39	0.5472297781422739
1103	40-41	0.5040256068052855
1103	42-43	0.5517918459252087
1103	44-45	0.46199094428409637
1103	46-47	0.5505195203392503
1103	48-49	0.5086932506858233
1103	50-51	0.5071728715214832
1103	52-53	0.4610532591564507
1103	54-55	0.4036950603508629
1103	56-57	0.4208718826964386
1103	58-59	0.3859106114367492
1103	60-61	0.42638556682387474
1103	62-63	0.389015461862364
1103	64-65	0.28125670280926585
1103	66-67	0.2557672328692604
1103	68-69	0.31557339916439275
1103	70-71	0.2965358732898409
1103	72-73	0.2963098281009273
1103	74-75	0.34137064865007716
1103	76-77	0.37159609229721013
1103	78-79	0.3908381825486984
1103	80-81	0.36593086231388483
1103	82-83	0.42760258569718346
1103	84-85	0.4131753834778067
1103	86-87	0.44771480030133404
1103	88-89	0.4445699290783729
1103	90-91	0.4397638371342474
1103	92-93	0.41028314289050627
1103	94-95	0.4828818933010268
1103	96-97	0.47727159921975115
1103	98-99	0.4280231572964013
1103	100	0.448430403544684
1104	1	1.5451180949778092
1104	2	1.754789134000326
1104	3	1.8044358731331158
1104	4	0.8641462258719557
1104	5	1.4905755139034156
1104	6	0.9395083216343352
1104	7	0.43510650899888503
1104	8	0.30470278895700176
1104	9	0.26749784919260833
1104	10-11	0.2019407295221498
1104	12-13	0.20149492961198234
1104	14-15	0.32930334457145705
1104	16-17	0.3307637774334182
1104	18-19	0.38261943137177923
1104	20-21	0.4413968148757732
1104	22-23	0.38794918388212096
1104	24-25	0.4380750453662685
1104	26-27	0.3644057327096988
1104	28-29	0.46236590926127263
1104	30-31	0.42721728038485907
1104	32-33	0.4297510629259449
1104	34-35	0.41371582226078374
1104	36-37	0.420182779162829
1104	38-39	0.4384384235643921
1104	40-41	0.41185197610813873
1104	42-43	0.354783252973192
1104	44-45	0.337115320441157
1104	46-47	0.3837979209966349
1104	48-49	0.3634634695002461
1104	50-51	0.3129275146140813
1104	52-53	0.30727627909777055
1104	54-55	0.2788368144899991
1104	56-57	0.23715426704136178
1104	58-59	0.21592255988317532
1104	60-61	0.2500033152847507
1104	62-63	0.2280844951470442
1104	64-65	0.27251148620340615
1104	66-67	0.2613914171308096
1104	68-69	0.17685795039538021
1104	70-71	0.08934586667753308
1104	72-73	0.09327591230326604
1104	74-75	0.15282498086465068
1104	76-77	0.19110955686419473
1104	78-79	0.1805413653979855
1104	80-81	0.22881243108463423
1104	82-83	0.2413422176204172
1104	84-85	0.21714468798373687
1104	86-87	0.19427987672977665
1104	88-89	0.20975063453834863
1104	90-91	0.1625133816105695
1104	92-93	0.14946468358878207
1104	94-95	0.1562965406842025
1104	96-97	0.18997705031778978
1104	98-99	0.2183983775138394
1104	100	0.2183654663226271
1105	1	2.1534382926886444
1105	2	1.9987378942974843
1105	3	1.939732984806902
1105	4	0.9959777993095855
1105	5	1.5625513048122954
1105	6	1.0579788175265818
1105	7	0.552802664110537
1105	8	0.3663602123666081
1105	9	0.2804960836316468
1105	10-11	0.2408754255388601
1105	12-13	0.19802936915912994
1105	14-15	0.3175075000270269
1105	16-17	0.28032609738097136
1105	18-19	0.26110638095032357
1105	20-21	0.35981034715518945
1105	22-23	0.37884075995464883
1105	24-25	0.4205881048965736
1105	26-27	0.331558678454968
1105	28-29	0.37087996987597904
1105	30-31	0.4084136228943578
1105	32-33	0.39411276942603735
1105	34-35	0.388318582152273
1105	36-37	0.3896175352756899
1105	38-39	0.3670154951770286
1105	40-41	0.30614126283883536
1105	42-43	0.3522635017735425
1105	44-45	0.39540562558527625
1105	46-47	0.4751910390190588
1105	48-49	0.37545199946357144
1105	50-51	0.40200820765952017
1105	52-53	0.34631141680463173
1105	54-55	0.35561634420250243
1105	56-57	0.3689729992277364
1105	58-59	0.39266594302856817
1105	60-61	0.5044179397370243
1105	62-63	0.49346319319968757
1105	64-65	0.4128737383455885
1105	66-67	0.46737581789870575
1105	68-69	0.4406141408004771
1105	70-71	0.4290371301166971
1105	72-73	0.496511574368629
1105	74-75	0.4844170360846931
1105	76-77	0.39581041014090346
1105	78-79	0.35158174446817014
1105	80-81	0.40411323961691537
1105	82-83	0.501750040652869
1105	84-85	0.5136070343469434
1105	86-87	0.4936415687890161
1105	88-89	0.49401539181315357
1105	90-91	0.5254168729423903
1105	92-93	0.5395154020878934
1105	94-95	0.5672493726355441
1105	96-97	0.5289374510536966
1105	98-99	0.4835748591842872
1105	100	0.4865440236991354
1106	1	1.6531496560743832
1106	2	1.1429087463139034
1106	3	1.5520013626213043
1106	4	0.7798569621776892
1106	5	1.4807161801139728
1106	6	0.960587826593212
1106	7	0.5061200548320883
1106	8	0.35869443358543407
1106	9	0.2612607398106448
1106	10-11	0.1728052054430833
1106	12-13	0.14255083762824228
1106	14-15	0.27174898456983954
1106	16-17	0.2530120759713128
1106	18-19	0.2324536958691965
1106	20-21	0.29302126940726225
1106	22-23	0.24377817832475301
1106	24-25	0.34700542462041284
1106	26-27	0.3079064374246343
1106	28-29	0.3522893172135113
1106	30-31	0.334257537277324
1106	32-33	0.34048372203634614
1106	34-35	0.31130422119681356
1106	36-37	0.2951418512159947
1106	38-39	0.3225759005979185
1106	40-41	0.3229899831262415
1106	42-43	0.3492903854654372
1106	44-45	0.3742662654491369
1106	46-47	0.34316405052685894
1106	48-49	0.2778426565485361
1106	50-51	0.18935866644358157
1106	52-53	0.27237361834433926
1106	54-55	0.23958616834038793
1106	56-57	0.12610783660035452
1106	58-59	0.13471042921066356
1106	60-61	0.17372002802901676
1106	62-63	0.15885581224725343
1106	64-65	0.114960020763192
1106	66-67	0.16462772333756703
1106	68-69	0.20382461576051725
1106	70-71	0.15945851306732095
1106	72-73	0.1457596303589348
1106	74-75	0.1912557927799412
1106	76-77	0.06798116736886683
1106	78-79	0.03325226425680938
1106	80-81	0.08239131941885347
1106	82-83	0.10606352768899896
1106	84-85	0.08611358472632347
1106	86-87	0.1465132880982125
1106	88-89	0.08913866974782181
1106	90-91	0.07471447678959109
1106	92-93	0.1538351360867054
1106	94-95	0.1453388695054727
1106	96-97	0.09658271490449621
1106	98-99	0.1276562167797195
1106	100	0.12919566020095985
1107	1	1.766972125232499
1107	2	1.1779865848957733
1107	3	1.4517507981893303
1107	4	0.7952761678889217
1107	5	1.483442909041905
1107	6	1.0107268131564453
1107	7	0.5790444885266695
1107	8	0.43201384514046026
1107	9	0.36446333883025517
1107	10-11	0.1894861678732198
1107	12-13	0.1408034348623417
1107	14-15	0.26947478753977094
1107	16-17	0.29348910375671977
1107	18-19	0.2721193390621863
1107	20-21	0.2577554111207334
1107	22-23	0.2909125922791702
1107	24-25	0.33451752109879607
1107	26-27	0.24972834568941948
1107	28-29	0.21468021675025994
1107	30-31	0.12628457738961174
1107	32-33	0.13948736533847494
1107	34-35	0.07830075647331114
1107	36-37	0.19520251194731486
1107	38-39	0.20346088006770913
1107	40-41	0.16499307721328194
1107	42-43	0.13777579451445376
1107	44-45	0.19167150544559775
1107	46-47	0.22013264694478352
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2216	1	-5.874728910408297
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2216	3	-5.7791081965079165
2216	4	-3.033779570748351
2216	5	-4.124060840744892
2216	6	-1.8699530273156668
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2216	12-13	-0.6143681921282536
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2216	56-57	-1.0536754633633194
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2216	64-65	-1.1849114406642016
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2216	84-85	-1.0484895588109602
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2216	90-91	-1.1332536772932436
2216	92-93	-1.1441669976351214
2216	94-95	-1.1861876482336342
2216	96-97	-1.181146183740296
2216	98-99	-1.2052443885543056
2216	100	-1.2961798354159022
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	9167.0
3	10525.0
4	11638.0
5	15234.0
6	12727.0
7	8899.0
8	7896.0
9	7986.0
10	8402.0
11	14139.0
12	9725.0
13	12277.0
14	11276.0
15	11619.0
16	13436.0
17	16917.0
18	23455.0
19	39481.0
20	82499.0
21	107459.0
22	48580.0
23	31758.0
24	32889.0
25	36530.0
26	37846.0
27	42126.0
28	48657.0
29	56876.0
30	67950.0
31	84381.0
32	110762.0
33	153597.0
34	229767.0
35	527657.0
36	662360.0
37	1115199.0
38	1215010.0
39	362063.0
40	2245.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	15.407732207843198	41.26785633390212	25.668970750343533	17.65544070791115
2	18.54652876004667	38.87177882135032	24.175345608157663	18.40634681044535
3	19.83348456237585	25.576918360840672	20.414449321921673	34.17514775486181
4	31.927500608374633	26.80076060977059	22.036498704963773	19.235240076891007
5	18.075140397773254	37.78417697759483	23.247135923154268	20.893546701477643
6	34.82132650193076	23.935136889007943	23.431761117221058	17.811775491840233
7	19.739955216081466	24.258377177179444	36.857051769379794	19.144615837359297
8	33.79855914250303	24.42332687544449	23.041231765267373	18.7368822167851
9	33.60069118903756	26.238490400885865	22.56264372261135	17.598174687465217
10-11	17.8092382395053	41.27259710885284	22.741751100262025	18.17641355137983
12-13	17.81147366256619	25.898800417278974	30.102169209263895	26.18755671089094
14-15	18.09978664443191	33.272300938877684	22.54159113074678	26.08632128594362
16-17	26.074890313272615	25.57735267607696	29.998588947079103	18.34916806357133
18-19	18.43237420793396	25.764061942912765	30.06121474964205	25.742349099511223
20-21	26.219384515432388	33.577702596909184	22.226482429521706	17.97643045813672
22-23	25.81511978871911	26.022014714204865	30.0439822674967	18.118883229579325
24-25	18.2955606573087	33.5001065834624	22.2908275970051	25.913505162223803
26-27	18.715371250379658	28.980742783731728	34.20765836551359	18.096227600375034
28-29	25.37770770657942	25.8295789015475	30.757773477716054	18.034939914157018
30-31	23.800657951084933	26.630804094102324	31.040845941421303	18.527692013391444
32-33	20.583842614845842	27.808987174330852	31.983139945956673	19.624030264866633
34-35	20.192418341194266	27.602521308653742	32.351688336579954	19.85337201357204
36-37	20.209008749306566	27.933448559467372	32.11643455263436	19.741108138591702
38-39	20.66851604087012	27.621337943342063	32.06134536549133	19.648800650296494
40-41	20.08291170045389	28.21778517286005	32.034581442116504	19.664721684569557
42-43	20.23348582802979	27.811351932976162	32.23098461189881	19.724177627095237
44-45	20.19743723972703	27.94271754457948	31.950735013228826	19.909110202464664
46-47	20.419489618166295	27.724266887811755	32.1167240846544	19.73951940936755
48-49	20.14830717308493	27.96193420926658	32.02230408582819	19.867454531820297
50-51	20.40470401704295	28.21756512745706	31.526607629368677	19.851123226131314
52-53	20.74652906327933	28.127880145754297	31.059123948031996	20.06646684293438
54-55	20.956778560155627	28.738529851531823	29.95149534025513	20.35319624805742
56-57	21.388669012904813	29.36100538618607	28.640486039530895	20.609839561378223
58-59	21.665819336667948	29.83758617534732	27.92025602885512	20.576338459129612
60-61	21.56395877950385	30.069855399248098	27.564320243652496	20.801865577595564
62-63	21.139866873763243	30.020071087814475	27.593647390819893	21.24641464760239
64-65	21.243601174361608	29.891425130297012	28.2651131517256	20.599860543615776
66-67	21.03237614309622	29.639296502766786	28.579322762744336	20.749004591392655
68-69	21.173586124150738	29.679693253541924	28.604963754174562	20.541756868132772
70-71	20.51115568989142	29.989459443193716	28.873654626308305	20.625730240606558
72-73	20.678745904373248	29.788308353342984	28.780503081058324	20.75244266122545
74-75	20.8066668685192	29.919233518764127	28.503237988194268	20.770861624522404
76-77	21.09543984126767	29.794816674440728	28.339616252236123	20.77012723205548
78-79	21.122616648991077	30.19085486777457	27.707398258125753	20.979130225108598
80-81	21.464630433304087	30.48812870616825	27.388424779859633	20.65881608066803
82-83	21.188277188900884	30.71150951746891	27.41441937383812	20.685793919792086
84-85	21.42656026049107	30.769111773175815	27.109474447755815	20.694853518577297
86-87	21.347047318777435	30.802662253097417	27.043434328379888	20.806856099745254
88-89	21.696755629029006	30.617516550862977	27.031403485637306	20.654324334470715
90-91	21.55756465319641	30.937584069624457	26.760193907708956	20.744657369470175
92-93	21.24732333037131	31.134926788958634	26.817107291295233	20.800642589374824
94-95	21.238021130522476	31.237801341242854	26.724735057229637	20.79944247100503
96-97	21.197428068398693	30.88802615324127	26.78739473539238	21.127151042967657
98-99	21.589888572558568	31.02020442402776	26.24750902843077	21.142397974982895
100	21.45613291086228	31.368268188125885	26.238002926308784	20.93759597470305
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	226202.0
1	122214.5
2	13118.5
3	6531.5
4	4656.5
5	4121.5
6	4091.5
7	4479.0
8	4283.5
9	3941.5
10	4095.0
11	6268.0
12	50686.5
13	55951.5
14	16705.5
15	14947.5
16	16288.0
17	18006.5
18	19805.5
19	21547.0
20	23315.5
21	25653.0
22	28659.5
23	32586.0
24	36698.0
25	41000.5
26	45975.5
27	50751.0
28	55142.0
29	59607.5
30	64530.0
31	70131.5
32	75753.0
33	80717.5
34	86249.5
35	91579.5
36	97196.5
37	104508.5
38	111831.5
39	118029.0
40	121669.5
41	123694.0
42	126517.0
43	130376.5
44	136214.5
45	142745.0
46	148962.5
47	156079.5
48	161078.0
49	168484.0
50	187819.5
51	191792.5
52	175555.5
53	170146.0
54	169708.0
55	170474.0
56	164892.5
57	150203.0
58	135080.5
59	115661.0
60	96515.0
61	80847.0
62	65980.5
63	51279.0
64	39141.5
65	29118.5
66	21482.0
67	16838.5
68	13113.0
69	9438.0
70	6757.5
71	5082.5
72	3831.5
73	2870.5
74	2094.5
75	1573.5
76	1117.5
77	717.0
78	463.0
79	338.0
80	239.5
81	130.5
82	62.5
83	24.0
84	14.5
85	11.0
86	8.5
87	5.5
88	1.5
89	3.0
90	2.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.059799924919968085
2	0.06808136562655041
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	7.545731851100073E-5
18-19	0.0
20-21	3.018292740440029E-4
22-23	1.8864329627750183E-4
24-25	0.0
26-27	0.0026410061478850254
28-29	9.903773054568846E-4
30-31	0.005885670843858057
32-33	0.0014714177109645143
34-35	0.0021128049183080206
36-37	0.007894721949213451
38-39	0.012422161059873494
40-41	0.003584222629272535
42-43	0.007328792060380946
44-45	0.0016034680183587655
46-47	0.0021882622368190213
48-49	0.003537061805203159
50-51	0.009790587076802344
52-53	0.003235232531159157
54-55	0.001433689051709014
56-57	7.923018443655078E-4
58-59	8.488948332487583E-4
60-61	0.0065270580512015635
62-63	0.0019147294572166434
64-65	0.008073933080677078
66-67	0.010347084800820975
68-69	0.011441215919230486
70-71	0.010016959032335347
72-73	0.004414253132893543
74-75	0.002084508423866395
76-77	0.004951886527284423
78-79	0.016166730490981905
80-81	0.00889453141948421
82-83	0.006338414754924062
84-85	0.004763243231006921
86-87	0.007300495565939321
88-89	0.004319931484754792
90-91	0.0064893293919460636
92-93	0.010941311184095108
94-95	0.009026581726878464
96-97	0.010526295932284602
98-99	0.0021976944016328962
100	0.0025278201701185244
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5301010.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.846007101671375
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.97770458908617	33.00307743626407
2	17.165820283632307	16.426319183933717
3	7.19731692921571	10.330886307247034
4	3.0190911898376105	5.778058340382552
5	1.3378464004153294	3.2005304187608665
6	0.688450208815169	1.9763756148070628
7	0.37558374141069373	1.257912765116585
8	0.24333325149114732	0.9314019583134577
9	0.16752264959203766	0.7213760893864295
>10	0.7014280190507738	5.3927519562061015
>50	0.05697248733089927	1.9905292057014052
>100	0.06456102286434144	4.959687055292668
>500	0.002661257332233389	0.8797588549061014
>1k	0.0013902080789847482	1.2485795666616146
>5k	1.5888092331201901E-4	0.651000670572098
>10k+	1.5888092331201901E-4	11.251754576448226
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	344657	6.501723256511495	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	220928	4.167658615999593	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	13739	0.2591770247556598	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	12732	0.24018064482051535	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC	9577	0.1806636848449635	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	9174	0.17306136000498018	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9161	0.17281612371981941	No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6343	0.11965644282881942	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.005847942184602557	0.0	0.0	0.0	0.0
2	0.005847942184602557	0.0	0.0	0.0	0.0
3	0.005847942184602557	0.0	0.0	0.0	0.0
4	0.005847942184602557	0.0	0.0	0.0	0.0
5	0.005847942184602557	0.0	0.0	0.0	0.0
6	0.005847942184602557	0.0	0.0	0.0	0.0
7	0.005847942184602557	0.0	0.0	0.0	0.0
8	0.005847942184602557	0.0	0.0	0.0	0.0
9	0.005847942184602557	0.0	0.0	0.0	0.0
10-11	0.005857374349416432	0.0	0.0	0.0	0.0
12-13	0.005866806514230307	0.0	0.0	0.0	0.0
14-15	0.005923399503113558	0.0	0.0	0.0	0.0
16-17	0.005979992491996808	0.0	0.0	0.0	0.0
18-19	0.006064881975321684	0.0	0.0	0.0	0.0
20-21	0.006130907129018809	0.0	0.0	0.0	0.0
22-23	0.006206364447529811	0.0	0.0	0.0	0.0
24-25	0.006244093106785311	0.0	0.0	0.0	0.0
26-27	0.006253525271599185	0.0	0.0	0.0	0.0
28-29	0.006300686095668561	0.0	0.0	0.0	0.0
30-31	0.006357279084551812	0.0	0.0	0.0	0.0
32-33	0.006451600732690563	0.0	0.0	0.0	0.0
34-35	0.00661194753452644	0.0	0.0	0.0	0.0
36-37	0.006923208973384318	0.0	0.0	0.0	0.0
38-39	0.007215606082614445	0.0	0.0	0.0	0.0
40-41	0.007630621334424948	0.0	0.0	0.0	0.0
42-43	0.008224847717699081	0.0	0.0	0.0	0.0
44-45	0.00878134544171771	0.0	0.0	0.0	0.0
46-47	0.00972456192310522	0.0	0.0	0.0	0.0
48-49	0.01076210005263148	0.0	0.0	0.0	0.0
50-51	0.012054306632132368	0.0	0.0	0.0	0.0
52-53	0.013770960628257634	0.0	0.0	0.0	0.0
54-55	0.015553639778080027	0.0	0.0	0.0	0.0
56-57	0.0180248669593153	0.0	0.0	0.0	0.0
58-59	0.02133555680898546	0.0	0.0	0.0	0.0
60-61	0.02569321695299575	0.0	0.0	0.0	0.0
62-63	0.03155059130241218	0.0	0.0	0.0	0.0
64-65	0.0390020015053735	0.0	9.432164813875091E-6	0.0	0.0
66-67	0.04827381951741272	0.0	1.8864329627750182E-5	0.0	0.0
68-69	0.06026210099584796	0.0	1.8864329627750182E-5	0.0	0.0
70-71	0.07657974612385188	0.0	1.8864329627750182E-5	0.0	0.0
72-73	0.09928296683084921	0.0	1.8864329627750182E-5	0.0	0.0
74-75	0.128598135072373	0.0	1.8864329627750182E-5	0.0	0.0
76-77	0.16538357784648586	0.0	1.8864329627750182E-5	0.0	0.0
78-79	0.21279907036583595	0.0	1.8864329627750182E-5	0.0	0.0
80-81	0.2736931264042135	0.0	1.8864329627750182E-5	0.0	0.0
82-83	0.34898066594856453	0.0	1.8864329627750182E-5	0.0	0.0
84-85	0.443707897174312	0.0	1.8864329627750182E-5	0.0	0.0
86-87	0.5624116913569301	0.0	1.8864329627750182E-5	0.0	0.0
88	0.6647601117522887	0.0	1.8864329627750182E-5	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGAGT	85070	0.0	80.750595	1
TACGAGT	3605	0.0	80.261894	1
ACGAGTG	91225	0.0	78.86255	2
CGAGTGG	94125	0.0	77.37597	3
GTGGAAT	98620	0.0	76.57964	6
TGGAATC	99985	0.0	75.93367	7
GAATCAC	100305	0.0	75.785126	9
GGAATCA	100515	0.0	75.63147	8
GAGTGGA	97770	0.0	75.48624	4
AGTGGAA	99165	0.0	74.320076	5
AACGTGT	2260	0.0	55.9994	1
CGTGTGG	3015	0.0	52.838173	3
ATCGAGT	1600	0.0	49.988316	1
TCGAGTG	1865	0.0	47.900455	2
ACGTGTG	3180	0.0	46.42665	2
ATCTGCG	2820	0.0	45.835823	94
AGTACTT	51265	0.0	41.66796	20-21
ACGATTG	750	0.0	41.375908	2
GAGTACT	50420	0.0	41.359673	20-21
GTACTTT	50775	0.0	40.00208	22-23
>>END_MODULE