FastQCFastQC Report
Thu 26 May 2016
SRR2132665_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132665_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4554893
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3184716.9918437161970655No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA198660.43614635953028974No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT130570.2866587645417796No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT70030.15374675102137414No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA61140.13422927827283757No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT58000.12733559273510925No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG50470.11080392009208558No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGAGT767150.087.612071
ACGAGTG823750.085.509192
CGAGTGG847300.084.192583
TACGAGT34850.083.705291
GAATCAC904050.082.2603459
TGGAATC904250.082.1330267
GAGTGGA880150.082.075394
GTGGAAT899300.082.072996
GGAATCA907150.081.93788
AGTGGAA890400.080.972235
CGTGTGG24450.065.156853
ATCGAGT12450.063.1118851
AACGTGT20200.061.7246361
ACGTGTG23400.059.6851122
TCGAGTG15450.056.9165422
CGATTGG7950.046.69813
GACGAGT9550.042.370061
AGTACTT488350.042.1975520-21
GAGTACT480000.041.903620-21
TGAGTAC904050.041.881318-19
Produced by FastQC (version 0.11.5)