##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2132665_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4554893 Sequences flagged as poor quality 0 Sequence length 100 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.173207142297304 31.0 28.0 34.0 16.0 34.0 2 30.30580323182125 33.0 30.0 34.0 22.0 34.0 3 30.770483741330477 33.0 31.0 34.0 25.0 34.0 4 35.237581431660416 37.0 35.0 37.0 32.0 37.0 5 34.708080299581134 37.0 35.0 37.0 32.0 37.0 6 35.28293332027778 37.0 35.0 37.0 32.0 37.0 7 35.68635706700465 37.0 35.0 37.0 35.0 37.0 8 35.908405971336755 37.0 35.0 37.0 35.0 37.0 9 37.83997779969804 39.0 39.0 39.0 35.0 39.0 10-11 37.945488071838355 39.0 39.0 39.0 35.0 39.0 12-13 37.96913956046827 39.0 39.0 39.0 35.0 39.0 14-15 39.41898843726955 41.0 39.0 41.0 37.0 41.0 16-17 39.40350596600183 41.0 39.0 41.0 37.0 41.0 18-19 39.417649766964885 41.0 39.5 41.0 37.0 41.0 20-21 39.32146397291879 41.0 39.0 41.0 36.0 41.0 22-23 39.25220877855968 40.5 39.0 41.0 36.0 41.0 24-25 39.22697141293989 41.0 39.0 41.0 36.0 41.0 26-27 39.17754094333281 41.0 39.0 41.0 36.0 41.0 28-29 39.01724178811665 41.0 39.0 41.0 36.0 41.0 30-31 38.909326190538394 41.0 39.0 41.0 35.0 41.0 32-33 38.76807512273065 41.0 39.0 41.0 35.0 41.0 34-35 38.710018105804025 41.0 39.0 41.0 35.0 41.0 36-37 38.588336323158416 40.0 38.5 41.0 35.0 41.0 38-39 38.169839225641525 40.0 37.0 41.0 34.5 41.0 40-41 37.970462752912084 40.0 37.0 41.0 34.0 41.0 42-43 37.84612602315795 40.0 37.0 41.0 34.0 41.0 44-45 37.723965085458644 40.0 36.5 41.0 33.0 41.0 46-47 37.602971793190314 40.0 36.0 41.0 33.0 41.0 48-49 37.47026691955223 40.0 36.0 41.0 33.0 41.0 50-51 37.22839833998296 40.0 35.5 41.0 33.0 41.0 52-53 36.75487602013922 40.0 35.0 41.0 31.5 41.0 54-55 35.91335197116595 40.0 35.0 41.0 28.5 41.0 56-57 35.189086659115816 39.5 35.0 41.0 23.5 41.0 58-59 34.837026907108466 39.0 35.0 41.0 21.5 41.0 60-61 34.59508873205145 39.0 35.0 41.0 20.0 41.0 62-63 34.29675889203105 39.0 35.0 41.0 18.0 41.0 64-65 34.04847797741901 38.0 35.0 40.0 17.5 41.0 66-67 33.76897931521114 38.0 34.0 40.0 14.5 41.0 68-69 33.456680541123575 37.0 34.0 40.0 12.0 41.0 70-71 33.10055351464897 36.5 34.0 39.5 8.0 41.0 72-73 32.73933954101666 36.0 34.0 39.0 7.0 41.0 74-75 32.36638588436655 36.0 33.5 39.0 3.5 40.5 76-77 31.978530231116295 35.0 33.0 38.0 2.0 40.0 78-79 31.83298180659787 35.0 33.5 37.0 2.0 39.0 80-81 31.663634689113444 35.0 34.0 37.0 2.0 39.0 82-83 31.33378808239842 35.0 33.5 36.5 2.0 39.0 84-85 31.03586395992178 35.0 33.0 36.0 2.0 37.5 86-87 30.80112299015586 35.0 33.0 36.0 2.0 37.0 88-89 30.56151955710046 35.0 33.0 35.5 2.0 37.0 90-91 30.353320703691615 35.0 33.0 35.0 2.0 36.0 92-93 30.170185007639038 35.0 33.0 35.0 2.0 36.0 94-95 29.997507735088398 35.0 33.0 35.0 2.0 36.0 96-97 29.837210116681113 35.0 33.0 35.0 2.0 36.0 98-99 29.681941705326558 35.0 33.0 35.0 2.0 36.0 100 29.560084506924753 35.0 33.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 2.8490658459049456 1101 2 2.273868020113788 1101 3 2.084059246370991 1101 4 1.0254502851083842 1101 5 1.6159237843131606 1101 6 1.0531266331343403 1101 7 0.5496739448373518 1101 8 0.45272008167742683 1101 9 0.3424036183788246 1101 10-11 0.32451826024155395 1101 12-13 0.2375504620428046 1101 14-15 0.3845628094327651 1101 16-17 0.3578690172151937 1101 18-19 0.35283102996708493 1101 20-21 0.3670108626740358 1101 22-23 0.31872308187030285 1101 24-25 0.3693865798579097 1101 26-27 0.3492838585770883 1101 28-29 0.36895071036410343 1101 30-31 0.3996671029313461 1101 32-33 0.39982870129669834 1101 34-35 0.41416327801191244 1101 36-37 0.3615896350659398 1101 38-39 0.3539293133722623 1101 40-41 0.3685721122605301 1101 42-43 0.35090744262222984 1101 44-45 0.3901938336506703 1101 46-47 0.39004939288408025 1101 48-49 0.33976787191731717 1101 50-51 0.3661194516747983 1101 52-53 0.3172039795641268 1101 54-55 0.30335363236068247 1101 56-57 0.20058120432452142 1101 58-59 0.18201493137322444 1101 60-61 0.14576770252056548 1101 62-63 0.1803161092880785 1101 64-65 0.17868328999328753 1101 66-67 0.1810976843543699 1101 68-69 0.1796079439279481 1101 70-71 0.1983500728269476 1101 72-73 0.17294617471301876 1101 74-75 0.238657955063168 1101 76-77 0.08373224486910047 1101 78-79 0.1392931127415622 1101 80-81 0.16287736090438898 1101 82-83 0.26542475679482536 1101 84-85 0.195757943469971 1101 86-87 0.21602654306102664 1101 88-89 0.16435054815198313 1101 90-91 0.06958771099336403 1101 92-93 0.06487998689459218 1101 94-95 0.1373319378033777 1101 96-97 0.14978545971463575 1101 98-99 0.15255034429293346 1101 100 0.12789861055924234 1102 1 2.8198659317152277 1102 2 2.2470756178232065 1102 3 2.034002735914605 1102 4 1.0064011062340157 1102 5 1.5518142681697213 1102 6 0.9893076948786828 1102 7 0.4461813795208087 1102 8 0.4052226778972212 1102 9 0.3399675709922505 1102 10-11 0.27015384661014963 1102 12-13 0.23639806572428768 1102 14-15 0.33547714250236993 1102 16-17 0.2501337514631814 1102 18-19 0.28045651986874987 1102 20-21 0.26888286283845275 1102 22-23 0.217099399895595 1102 24-25 0.26092964653278017 1102 26-27 0.21041722529241724 1102 28-29 0.2853291629457644 1102 30-31 0.29680157522695794 1102 32-33 0.30237177374660007 1102 34-35 0.26495785970035257 1102 36-37 0.2524958550548462 1102 38-39 0.2361662866674621 1102 40-41 0.23028863053699666 1102 42-43 0.27226198314482986 1102 44-45 0.2577707215024674 1102 46-47 0.19033335920133254 1102 48-49 0.1821223613138443 1102 50-51 0.1784126118543341 1102 52-53 0.13416661981689515 1102 54-55 0.14570413018604 1102 56-57 0.21338469961599316 1102 58-59 0.17329029101379234 1102 60-61 0.10104318802301293 1102 62-63 0.13665547589537397 1102 64-65 0.1880188955214237 1102 66-67 0.18974037011444977 1102 68-69 0.21527394861660554 1102 70-71 0.19522870664292213 1102 72-73 0.18304315120826686 1102 74-75 0.258853139495848 1102 76-77 0.17569677050228805 1102 78-79 0.24274845878020557 1102 80-81 0.2126536758043649 1102 82-83 0.24709737288415212 1102 84-85 0.20789026031715707 1102 86-87 0.17087643790436147 1102 88-89 0.21047054140537824 1102 90-91 0.19893459568248062 1102 92-93 0.22635037809913783 1102 94-95 0.2215660852360486 1102 96-97 0.15489579608348691 1102 98-99 0.18215652823879935 1102 100 0.1643067927026145 1103 1 2.6091357442837264 1103 2 2.2337583913682266 1103 3 2.0239423421762197 1103 4 0.9997405216335196 1103 5 1.6175498463725901 1103 6 1.030831990370146 1103 7 0.5390600248696416 1103 8 0.4129903025736965 1103 9 0.4078566355662119 1103 10-11 0.2781127359709714 1103 12-13 0.17598793381328903 1103 14-15 0.3235915478769229 1103 16-17 0.33685521787251815 1103 18-19 0.3300424651329763 1103 20-21 0.37782960928858245 1103 22-23 0.3201585474772273 1103 24-25 0.36511181268739534 1103 26-27 0.3312406018086378 1103 28-29 0.3843334245146721 1103 30-31 0.3478075523469144 1103 32-33 0.3868459786835672 1103 34-35 0.37543567818478607 1103 36-37 0.33482757180696865 1103 38-39 0.34380719563726103 1103 40-41 0.2877831432721081 1103 42-43 0.3254075640196348 1103 44-45 0.30343865629460254 1103 46-47 0.3031271051927007 1103 48-49 0.2948282255716492 1103 50-51 0.2444764132873516 1103 52-53 0.21997930402095278 1103 54-55 0.1141320726843702 1103 56-57 0.058063478287749604 1103 58-59 0.04638161804331986 1103 60-61 0.06536351546847641 1103 62-63 0.019232261289054975 1103 64-65 0.03758902414873688 1103 66-67 0.06026864246706509 1103 68-69 0.09224290409852642 1103 70-71 0.026589906087693294 1103 72-73 0.05407074121679756 1103 74-75 0.07524171944406532 1103 76-77 0.10118148547205053 1103 78-79 0.06261555752757175 1103 80-81 0.07843654205787232 1103 82-83 0.1435081334763808 1103 84-85 0.18057510268326382 1103 86-87 0.19098979641759328 1103 88-89 0.2053379783988447 1103 90-91 0.2179175689130588 1103 92-93 0.21762265326529118 1103 94-95 0.23923442243728488 1103 96-97 0.2328652171414518 1103 98-99 0.1701689409993179 1103 100 0.22632332384810994 1104 1 1.5794917648070879 1104 2 1.77539725815042 1104 3 1.8222149040709894 1104 4 0.9021065037776523 1104 5 1.5751707924770528 1104 6 1.0397828915886649 1104 7 0.5100271432905075 1104 8 0.41586270977204265 1104 9 0.3521463151689659 1104 10-11 0.2755125219105139 1104 12-13 0.24379837332743648 1104 14-15 0.3742789530143753 1104 16-17 0.30936059206679545 1104 18-19 0.3129624267407891 1104 20-21 0.3531999782787878 1104 22-23 0.25023186707344536 1104 24-25 0.3118133786728521 1104 26-27 0.21794709400381862 1104 28-29 0.3149391004220803 1104 30-31 0.30466892972843596 1104 32-33 0.2623297290772797 1104 34-35 0.20710743173075485 1104 36-37 0.2246621585789086 1104 38-39 0.2084395129340919 1104 40-41 0.21299779510467687 1104 42-43 0.18117321010245035 1104 44-45 0.24698893863016735 1104 46-47 0.2201682872043662 1104 48-49 0.16681569156371268 1104 50-51 0.235448755224148 1104 52-53 0.2379199911919727 1104 54-55 0.2195797348045403 1104 56-57 0.11461142041041228 1104 58-59 0.16093879869272598 1104 60-61 0.10107035406183229 1104 62-63 0.06980856140013003 1104 64-65 0.06568569499983568 1104 66-67 0.09589285100070555 1104 68-69 0.03824576002021729 1104 70-71 -0.024801231724993045 1104 72-73 -0.03739044663404911 1104 74-75 0.019757517825418347 1104 76-77 0.10475649120755648 1104 78-79 0.11658728476152902 1104 80-81 0.0465626040971685 1104 82-83 0.07642568909126979 1104 84-85 0.10785410858856181 1104 86-87 0.05486143110588415 1104 88-89 0.020006308630041048 1104 90-91 0.017579692978692663 1104 92-93 0.05851545566862981 1104 94-95 0.038796458817841994 1104 96-97 0.0831074487817034 1104 98-99 0.1341440374334617 1104 100 0.07674298733962104 1105 1 2.2440030343749875 1105 2 2.0044511242072716 1105 3 1.9660978784732919 1105 4 0.9554569611405412 1105 5 1.5563205666388953 1105 6 1.0085328915886649 1105 7 0.48307939070514294 1105 8 0.3692904435917086 1105 9 0.33022356319288093 1105 10-11 0.2355615584426758 1105 12-13 0.19529597278152266 1105 14-15 0.2683697157868252 1105 16-17 0.22698322909288038 1105 18-19 0.21181505921760646 1105 20-21 0.3040555535568217 1105 22-23 0.23382445564180898 1105 24-25 0.3139878579452997 1105 26-27 0.18361085916463082 1105 28-29 0.21090744753858104 1105 30-31 0.25295221953241054 1105 32-33 0.24433045817708887 1105 34-35 0.2300519896809874 1105 36-37 0.24837656165127697 1105 38-39 0.24552281918851548 1105 40-41 0.12776851453732263 1105 42-43 0.19434054460288763 1105 44-45 0.23726223428319315 1105 46-47 0.28462850455328237 1105 48-49 0.20969587446825244 1105 50-51 0.19417490208395094 1105 52-53 0.2061031110080478 1105 54-55 0.22234609994747956 1105 56-57 0.31756404618520406 1105 58-59 0.30397944956155243 1105 60-61 0.38782566694517584 1105 62-63 0.4380601993360713 1105 64-65 0.3683769923100826 1105 66-67 0.30617391999507504 1105 68-69 0.26420463290094887 1105 70-71 0.24048688963458176 1105 72-73 0.3610752958292096 1105 74-75 0.3880997855086292 1105 76-77 0.35934357497076164 1105 78-79 0.2765366031852139 1105 80-81 0.295887577492131 1105 82-83 0.39495218648763597 1105 84-85 0.39313281663993394 1105 86-87 0.31071428495285147 1105 88-89 0.2997335003975081 1105 90-91 0.3346863243344522 1105 92-93 0.34395130605491175 1105 94-95 0.33897157478330087 1105 96-97 0.37428826045867325 1105 98-99 0.36379943622466016 1105 100 0.35422842565249724 1106 1 1.5511203242507072 1106 2 1.0814369057803894 1106 3 1.5372482359078745 1106 4 0.7669099397798362 1106 5 1.4777097165117041 1106 6 0.9585228217356843 1106 7 0.5233309233200245 1106 8 0.39194741297949065 1106 9 0.3506391886435978 1106 10-11 0.2735203940102835 1106 12-13 0.20698136967921954 1106 14-15 0.2724866614244803 1106 16-17 0.26636152095617405 1106 18-19 0.26367396473468574 1106 20-21 0.3116028146414962 1106 22-23 0.22129092839791298 1106 24-25 0.3443352529343642 1106 26-27 0.23744118365482336 1106 28-29 0.2847313828150533 1106 30-31 0.2486565513694572 1106 32-33 0.22885415127659314 1106 34-35 0.21996927225670504 1106 36-37 0.18566937107213022 1106 38-39 0.1776104670822818 1106 40-41 0.2700071337242136 1106 42-43 0.301569321253055 1106 44-45 0.2961569345641948 1106 46-47 0.26907062451700625 1106 48-49 0.258619850303873 1106 50-51 0.16835146205051643 1106 52-53 0.19513133870042054 1106 54-55 0.12639550154435142 1106 56-57 0.05557459834065526 1106 58-59 0.08585814634083277 1106 60-61 0.03376591575074883 1106 62-63 0.06068769658681816 1106 64-65 -0.01290260007429822 1106 66-67 -0.016887573360982344 1106 68-69 -0.007654736192833411 1106 70-71 -0.005663220882595965 1106 72-73 -0.0718095860263901 1106 74-75 0.018268872831697536 1106 76-77 -0.003697832344617069 1106 78-79 -0.0031765280838627064 1106 80-81 0.004988783501417515 1106 82-83 0.06934688251537935 1106 84-85 0.03430592301706881 1106 86-87 0.0010889422871613874 1106 88-89 -0.059146934813487206 1106 90-91 0.0210354763477838 1106 92-93 0.04301855012198175 1106 94-95 0.026169256777929917 1106 96-97 0.032144980885206564 1106 98-99 0.059645412061918535 1106 100 0.08322954704761543 1107 1 1.5536352546299383 1107 2 1.0467538360009314 1107 3 1.332698391771359 1107 4 0.6861964778156917 1107 5 1.4108532052476335 1107 6 0.9151165866155964 1107 7 0.4526750095802541 1107 8 0.3859293411152649 1107 9 0.34446392777589807 1107 10-11 0.19890510660219007 1107 12-13 0.1408798059109273 1107 14-15 0.22564317680094348 1107 16-17 0.20082400154291236 1107 18-19 0.17933518852132835 1107 20-21 0.18612416335312076 1107 22-23 0.20266400338297075 1107 24-25 0.23846388768416915 1107 26-27 0.18317509939308252 1107 28-29 0.17191521441785795 1107 30-31 0.11222484291889856 1107 32-33 0.16272340730890988 1107 34-35 0.07429559981292755 1107 36-37 0.15637678809802225 1107 38-39 0.16894436003844504 1107 40-41 0.1813181846263845 1107 42-43 0.14056889661792127 1107 44-45 0.16035424442146962 1107 46-47 0.21880590905486486 1107 48-49 0.21593913892152727 1107 50-51 0.2178774828727157 1107 52-53 0.12713535704337886 1107 54-55 0.16300094225107387 1107 56-57 0.21731467311504105 1107 58-59 0.253857781377576 1107 60-61 0.25623215875651795 1107 62-63 0.29153983241347703 1107 64-65 0.2606964669836742 1107 66-67 0.2554080521290061 1107 68-69 0.22210136698872418 1107 70-71 0.23240995106690576 1107 72-73 0.20881665383934944 1107 74-75 0.1742879704525322 1107 76-77 0.25521857336301323 1107 78-79 0.22638267666717837 1107 80-81 0.2672251839594182 1107 82-83 0.30522906645330394 1107 84-85 0.329377110994038 1107 86-87 0.32615933307794975 1107 88-89 0.2860687630658738 1107 90-91 0.27438109627807705 1107 92-93 0.3397189206215181 1107 94-95 0.35607106423601564 1107 96-97 0.32956145127639047 1107 98-99 0.3000586486500154 1107 100 0.303745503558158 1108 1 0.5906993271498386 1108 2 0.1636519827006886 1108 3 -0.017488620294066948 1108 4 0.044630258208833595 1108 5 1.0757912954573072 1108 6 0.730077183788076 1108 7 0.3420947679331334 1108 8 0.2850912281961939 1108 9 0.2476399859620102 1108 10-11 0.1508480758355546 1108 12-13 0.11932172414458364 1108 14-15 0.13583106485282315 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-0.7573943258297362 2215 44-45 -0.8048582298233384 2215 46-47 -0.7303250944626001 2215 48-49 -0.8832305020685212 2215 50-51 -0.7941753474064726 2215 52-53 -0.8478034169907431 2215 54-55 -0.8407973536527535 2215 56-57 -0.852397548918141 2215 58-59 -0.7832939660780127 2215 60-61 -0.784089594702678 2215 62-63 -0.6831272988272588 2215 64-65 -0.6744168165119362 2215 66-67 -0.6957370748649865 2215 68-69 -0.6923335046981407 2215 70-71 -0.7338720054207784 2215 72-73 -0.7198679219766646 2215 74-75 -0.715344295247359 2215 76-77 -0.681897056862514 2215 78-79 -0.6427385906569114 2215 80-81 -0.5949724712594104 2215 82-83 -0.6144431877190577 2215 84-85 -0.6192801993581796 2215 86-87 -0.6076056113043293 2215 88-89 -0.6614058134022187 2215 90-91 -0.606323383645055 2215 92-93 -0.6081360625631227 2215 94-95 -0.6077845264301267 2215 96-97 -0.6375252964078015 2215 98-99 -0.6356078327616501 2215 100 -0.6676464273186156 2216 1 -5.851472287480494 2216 2 -4.7301611664457255 2216 3 -5.89184421455974 2216 4 -3.0352275017309083 2216 5 -4.276451308425738 2216 6 -2.0165507204514697 2216 7 -1.2503710795437755 2216 8 -0.8280897625212233 2216 9 -0.6281604535808967 2216 10-11 -0.9667407269364006 2216 12-13 -0.7833297716875336 2216 14-15 -1.648758418288665 2216 16-17 -1.1433275623477073 2216 18-19 -1.6672924260364468 2216 20-21 -1.1098218455566524 2216 22-23 -0.7926254514392426 2216 24-25 -1.4119854496656217 2216 26-27 -1.1838832172734186 2216 28-29 -1.8972623144247933 2216 30-31 -1.9181523754937686 2216 32-33 -1.0937561208145183 2216 34-35 -1.0583683098479284 2216 36-37 -1.0608562224078284 2216 38-39 -1.0123628757471295 2216 40-41 -1.0499303067380623 2216 42-43 -1.3784369146051745 2216 44-45 -1.2325264530895055 2216 46-47 -1.6076667347642868 2216 48-49 -1.2800240602232904 2216 50-51 -1.1291782968229782 2216 52-53 -0.9386086609600852 2216 54-55 -0.9098679612192342 2216 56-57 -0.7521178869758671 2216 58-59 -0.6982408333050003 2216 60-61 -0.7030421423860176 2216 62-63 -0.685836617518703 2216 64-65 -0.7914425729740202 2216 66-67 -0.7338026071714125 2216 68-69 -0.7454941573514162 2216 70-71 -0.699111500704376 2216 72-73 -0.7105541240336635 2216 74-75 -0.6657580624312054 2216 76-77 -0.7485033537003112 2216 78-79 -0.685883710604287 2216 80-81 -0.6386315753025755 2216 82-83 -0.5623883998107324 2216 84-85 -0.5363360159004777 2216 86-87 -0.5539083379500092 2216 88-89 -0.6450373007869707 2216 90-91 -0.6697642175509593 2216 92-93 -0.6812253333448588 2216 94-95 -0.6110825633038282 2216 96-97 -0.6004377153820677 2216 98-99 -0.6673361142704763 2216 100 -0.6950603549560483 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5031.0 3 1550.0 4 1602.0 5 2027.0 6 2109.0 7 1966.0 8 2304.0 9 2885.0 10 3690.0 11 8315.0 12 6214.0 13 8997.0 14 8611.0 15 9028.0 16 11242.0 17 14664.0 18 21333.0 19 37947.0 20 83291.0 21 115368.0 22 53282.0 23 32246.0 24 29687.0 25 30630.0 26 31466.0 27 34302.0 28 39314.0 29 46212.0 30 54549.0 31 67022.0 32 85519.0 33 113586.0 34 162777.0 35 270229.0 36 455257.0 37 847966.0 38 1307658.0 39 542071.0 40 2946.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 14.207284269362042 41.900947521337514 27.389611995800706 16.502156213499735 2 17.477201143353515 39.475464275914554 25.87135010137585 17.175984479356078 3 18.840091304010873 24.703675805337248 21.391215995633704 35.06501689501817 4 33.090546803185056 25.431530444293642 23.424523913075454 18.053398839445844 5 17.526339257585192 38.417126373769925 24.43490988701601 19.621624481628878 6 35.75532948853024 22.66048840225226 24.784643678786747 16.799538430430747 7 18.764041218970455 22.827385846385415 40.22388670820588 18.184686226438252 8 34.88826191965431 22.904819059415885 24.424503495471793 17.782415525458006 9 34.56750795243708 24.885370523522727 23.87772885114974 16.669392672890453 10-11 16.84932006086641 41.80349571329118 24.20313715382557 17.14404707201684 12-13 16.74198493795573 24.386555293395475 32.67986756220179 26.191592206447 14-15 17.004603620765625 32.787751545425984 24.17047996517152 26.037164868636868 16-17 25.925115644944224 24.04772672885624 32.687320659774436 17.339836966425096 18-19 17.26982390146157 24.16307035093909 32.798684842871175 25.768420904728167 20-21 26.038647581630904 32.9997129450792 23.98250007821286 16.979139395077038 22-23 25.673419707979516 24.41050116292702 32.78798854680446 17.128090582288998 24-25 17.110182390673064 32.86285759072716 24.082212249552295 25.94474776904748 26-27 17.39184510713673 27.904806525530173 37.576257682302156 17.127090685030936 28-29 25.211235910653674 24.155517403388906 33.546036541188975 17.087210144768445 30-31 23.258001616383925 25.1210037476307 33.970606929988094 17.650387705997282 32-33 19.634041146220856 26.802466129903014 34.99360788297431 18.56988484090182 34-35 19.198585839159072 26.46688560121156 35.37709565279247 18.957432906836896 36-37 19.060871100232614 26.856228732121863 35.34034807606151 18.74255209158401 38-39 19.390451678009512 26.698997736115192 35.17974450521311 18.73080608066219 40-41 19.15560421784037 27.119557437645287 35.029698679268314 18.695139665246025 42-43 19.274512983252645 26.84145634449842 35.14296337907194 18.741067293176997 44-45 19.078899414509316 26.978797368585134 35.08434596202873 18.85795725487682 46-47 19.238039263513553 26.917638436998775 35.07485583578689 18.769466463700784 48-49 19.10402130527758 27.168641898330094 34.92152131625515 18.805815480137174 50-51 19.289209365713436 27.303973343951487 34.51891754100126 18.887899749333815 52-53 19.383122571489167 27.419388479621038 34.05345764615634 19.144031302733456 54-55 19.86289482723209 28.054867975703562 32.755323503682746 19.326913693381602 56-57 20.22364456087194 28.74263026438894 31.341611594556717 19.692113580182404 58-59 20.543220902639945 29.43387494276706 30.464029499980295 19.558874654612705 60-61 20.38311145332912 29.6693135502634 30.235411374628494 19.712163621778984 62-63 20.105435780478683 29.883416664644997 30.150332269586187 19.860815285290133 64-65 20.134854674985853 29.708691287195776 30.372436688068422 19.784017349749945 66-67 20.035752388743937 29.47138151155997 30.61469824329585 19.878167856400246 68-69 20.123413973881874 29.510688306214732 30.613030870619966 19.752866849283425 70-71 19.864319139300957 29.700671048839734 30.5704601281717 19.864549683687613 72-73 19.85676505145417 29.520617728845984 30.668809222680515 19.953807997019332 74-75 19.969271997054538 29.534515543262792 30.442216710580016 20.05399574910265 76-77 20.074648452444272 29.56965716051558 30.456941839524486 19.89875254751566 78-79 20.101078935791392 29.887585283224897 30.039643943393774 19.971691837589933 80-81 20.08903311790466 30.02472288304825 29.89515828902719 19.991085710019902 82-83 20.16248841967843 30.086100836703277 29.777138570739943 19.97427217287835 84-85 20.146757274165257 30.26790014234861 29.552435651942233 20.032906931543902 86-87 20.20959903094782 30.43251111223087 29.35161481800576 20.00627503881555 88-89 20.421868744456976 30.25105547322931 29.164663703653616 20.162412078660104 90-91 20.408946729275858 30.538314755424345 28.988322363112655 20.06441615218714 92-93 20.291906439140398 30.647047270395067 28.829257480313835 20.231788810150704 94-95 20.324165858764193 30.780639630413027 28.699824645550287 20.195369865272497 96-97 20.268108117128207 30.606728359469997 28.710153315677122 20.41501020772467 98-99 20.457766338784808 30.739917594462625 28.312901733581192 20.48941433317138 100 20.52662570157189 30.89072296044676 28.200994473488468 20.38165686449289 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 17967.0 1 10312.0 2 2156.0 3 1492.5 4 1328.0 5 1282.0 6 1237.0 7 1323.5 8 1289.0 9 1275.5 10 1434.5 11 2105.0 12 18770.5 13 23555.0 14 12598.5 15 14101.5 16 16669.5 17 19306.0 18 21785.5 19 24487.5 20 27760.5 21 31400.5 22 35671.5 23 40946.0 24 46732.0 25 53418.5 26 61924.5 27 70319.0 28 77350.5 29 84403.0 30 92152.5 31 100155.5 32 108946.5 33 118908.5 34 128375.5 35 135421.0 36 141369.5 37 146262.0 38 149027.0 39 151289.5 40 153663.0 41 155897.5 42 155985.5 43 154396.5 44 153697.5 45 152164.5 46 148762.0 47 145977.0 48 142041.0 49 137262.0 50 135772.5 51 126851.0 52 111262.5 53 100566.0 54 91840.0 55 83210.0 56 74163.0 57 65268.0 58 60052.0 59 51781.5 60 40067.0 61 31520.0 62 24223.0 63 18196.0 64 13498.0 65 10064.0 66 7474.0 67 5447.5 68 4119.0 69 3163.0 70 2343.0 71 1736.5 72 1351.0 73 1077.0 74 792.5 75 578.5 76 433.5 77 324.5 78 237.5 79 137.5 80 69.0 81 39.0 82 24.0 83 15.5 84 7.5 85 5.0 86 3.5 87 2.0 88 0.5 89 1.0 90 1.5 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05927691385944742 2 0.06630232587241895 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 6.586323762160824E-5 18-19 0.0 20-21 3.4029339437830923E-4 22-23 1.5368088778375255E-4 24-25 0.0 26-27 0.002327167729296824 28-29 7.244956138376906E-4 30-31 0.006191144336431175 32-33 0.001405082402594309 34-35 0.0023491221418373604 36-37 0.007519386295133606 38-39 0.01215176734118672 40-41 0.003183389818377731 42-43 0.007738930420538968 44-45 0.0017783074157834223 46-47 0.00229423611048602 48-49 0.0033590251187020203 50-51 0.009429420186160246 52-53 0.0032272986434588036 54-55 0.0015038772590267213 56-57 6.915639950268865E-4 58-59 9.330625329727834E-4 60-61 0.006399711255566267 62-63 0.0018112390345942264 64-65 0.007914565720863256 66-67 0.011240659220754472 68-69 0.011998086453402966 70-71 0.010000234912214183 72-73 0.004577495014701773 74-75 0.001975897128648247 76-77 0.0046214038397828444 78-79 0.01601574394832107 80-81 0.00895740031653872 82-83 0.006399711255566267 84-85 0.00503853767805303 86-87 0.007453523057511998 88-89 0.004006680288647834 90-91 0.006366779636755462 92-93 0.010724730526051874 94-95 0.008858605460106308 96-97 0.010955251857727503 98-99 0.00223935007913468 100 0.0026564839174048655 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4554893.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.83025120269588 #Duplication Level Percentage of deduplicated Percentage of total 1 91.01476082009934 67.19642654501217 2 6.65866701898465 9.832221173734855 3 0.9116514580030125 2.0192236846099907 4 0.2994064629104483 0.8842101747355618 5 0.1813243786376338 0.6693612211994622 6 0.12568780409478772 0.5567737289660042 7 0.0971320255825395 0.5019897294009901 8 0.07774391959150138 0.4591882490338189 9 0.06614926281982043 0.43954350217744437 >10 0.5439473068424902 6.573525117948059 >50 0.01671939941949054 0.8014813436788852 >100 0.005766434970286498 0.9968406190476037 >500 4.473036129960325E-4 0.2390976138027136 >1k 3.8766288014519847E-4 0.5661062655959936 >5k 1.1928088619849471E-4 0.5275996791016483 >10k+ 8.946066464887104E-5 7.73641135195479 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 318471 6.9918437161970655 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 19866 0.43614635953028974 No Hit TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 13057 0.2866587645417796 No Hit ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 7003 0.15374675102137414 No Hit AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA 6114 0.13422927827283757 No Hit AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 5800 0.12733559273510925 No Hit AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG 5047 0.11080392009208558 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0012294471022700204 0.0 0.0 0.0 0.0 2 0.0012514015148105565 0.0 0.0 0.0 0.0 3 0.0012514015148105565 0.0 0.0 0.0 0.0 4 0.0012514015148105565 0.0 0.0 0.0 0.0 5 0.0012514015148105565 0.0 0.0 0.0 2.1954412540536078E-5 6 0.0012514015148105565 0.0 0.0 0.0 2.1954412540536078E-5 7 0.0012514015148105565 0.0 0.0 2.1954412540536078E-5 2.1954412540536078E-5 8 0.0012514015148105565 0.0 0.0 2.1954412540536078E-5 2.1954412540536078E-5 9 0.0012514015148105565 0.0 0.0 2.1954412540536078E-5 2.1954412540536078E-5 10-11 0.0012514015148105565 0.0 0.0 8.781765016214431E-5 2.1954412540536078E-5 12-13 0.0012514015148105565 0.0 0.0 1.5368088778375255E-4 4.3908825081072156E-5 14-15 0.0012514015148105565 0.0 0.0 1.975897128648247E-4 4.3908825081072156E-5 16-17 0.0012843331336213604 0.0 0.0 2.4149853794589685E-4 4.3908825081072156E-5 18-19 0.0013172647524321646 2.1954412540536078E-5 0.0 2.4149853794589685E-4 4.3908825081072156E-5 20-21 0.0013172647524321646 4.3908825081072156E-5 0.0 2.524757442161649E-4 6.586323762160824E-5 22-23 0.001427036815134845 4.3908825081072156E-5 0.0 2.85407363026969E-4 6.586323762160824E-5 24-25 0.001448991227675381 4.3908825081072156E-5 0.0 2.85407363026969E-4 6.586323762160824E-5 26-27 0.0014819228464861853 4.3908825081072156E-5 0.0 3.073617755675051E-4 6.586323762160824E-5 28-29 0.0015587632903780616 4.3908825081072156E-5 0.0 3.073617755675051E-4 6.586323762160824E-5 30-31 0.0015807177029185976 4.3908825081072156E-5 0.0 3.5127060064857725E-4 6.586323762160824E-5 32-33 0.0016246265279996697 4.3908825081072156E-5 0.0 3.951794257296494E-4 8.781765016214431E-5 34-35 0.001690489765621278 4.3908825081072156E-5 0.0 3.951794257296494E-4 8.781765016214431E-5 36-37 0.0018331934471347625 6.586323762160824E-5 0.0 3.951794257296494E-4 8.781765016214431E-5 38-39 0.0021954412540536082 6.586323762160824E-5 0.0 3.951794257296494E-4 8.781765016214431E-5 40-41 0.00274430156756701 7.684044389187628E-5 0.0 4.061566319999174E-4 8.781765016214431E-5 42-43 0.0035566148315668445 8.781765016214431E-5 0.0 4.171338382701855E-4 1.0977206270268039E-4 44-45 0.004379905301836948 1.3172647524321648E-4 0.0 4.6104266335125765E-4 1.3172647524321648E-4 46-47 0.0054666487225934835 1.3172647524321648E-4 0.0 4.829970758917937E-4 1.4270368151348453E-4 48-49 0.006981503187890473 1.5368088778375255E-4 0.0 5.049514884323297E-4 1.7563530032428863E-4 50-51 0.008474403240646926 1.8661250659455668E-4 0.0 5.269059009728659E-4 1.7563530032428863E-4 52-53 0.010230756243889812 2.0856691913509272E-4 0.0 5.269059009728659E-4 1.7563530032428863E-4 54-55 0.013238510761943255 2.1954412540536078E-4 0.0 5.48860313513402E-4 1.7563530032428863E-4 56-57 0.0170695557502668 2.1954412540536078E-4 0.0 5.708147260539381E-4 1.7563530032428863E-4 58-59 0.021614119146157768 2.1954412540536078E-4 0.0 5.927691385944742E-4 1.7563530032428863E-4 60-61 0.027783309070048407 2.1954412540536078E-4 0.0 6.147235511350102E-4 1.7563530032428863E-4 62-63 0.035577125521938716 2.4149853794589685E-4 0.0 6.476551699458143E-4 1.7563530032428863E-4 64-65 0.04705928328063908 2.4149853794589685E-4 0.0 6.586323762160823E-4 1.975897128648247E-4 66-67 0.06146137790723075 2.4149853794589685E-4 0.0 6.696095824863504E-4 1.975897128648247E-4 68-69 0.07900295352711909 2.6345295048643295E-4 0.0 7.025412012971545E-4 1.975897128648247E-4 70-71 0.10232951685143866 2.6345295048643295E-4 0.0 7.244956138376906E-4 2.3052133167562883E-4 72-73 0.13192406495608128 2.6345295048643295E-4 0.0 7.244956138376906E-4 2.524757442161649E-4 74-75 0.1711017141346679 2.6345295048643295E-4 0.0 7.244956138376906E-4 2.85407363026969E-4 76-77 0.22205790564125216 2.6345295048643295E-4 0.0 7.244956138376906E-4 2.85407363026969E-4 78-79 0.2873832601556172 2.6345295048643295E-4 0.0 7.354728201079586E-4 2.963845692972371E-4 80-81 0.3692512645192763 2.74430156756701E-4 0.0 7.793816451890308E-4 3.073617755675051E-4 82-83 0.4704062202997963 3.073617755675051E-4 0.0 7.903588514592988E-4 3.402933943783092E-4 84-85 0.5946791724855007 3.073617755675051E-4 0.0 8.013360577295668E-4 3.5127060064857725E-4 86-87 0.742695821833795 3.073617755675051E-4 0.0 8.123132639998349E-4 3.5127060064857725E-4 88 0.875058975040687 3.073617755675051E-4 0.0 8.123132639998349E-4 3.5127060064857725E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACGAGT 76715 0.0 87.61207 1 ACGAGTG 82375 0.0 85.50919 2 CGAGTGG 84730 0.0 84.19258 3 TACGAGT 3485 0.0 83.70529 1 GAATCAC 90405 0.0 82.260345 9 TGGAATC 90425 0.0 82.133026 7 GAGTGGA 88015 0.0 82.07539 4 GTGGAAT 89930 0.0 82.07299 6 GGAATCA 90715 0.0 81.9378 8 AGTGGAA 89040 0.0 80.97223 5 CGTGTGG 2445 0.0 65.15685 3 ATCGAGT 1245 0.0 63.111885 1 AACGTGT 2020 0.0 61.724636 1 ACGTGTG 2340 0.0 59.685112 2 TCGAGTG 1545 0.0 56.916542 2 CGATTGG 795 0.0 46.6981 3 GACGAGT 955 0.0 42.37006 1 AGTACTT 48835 0.0 42.19755 20-21 GAGTACT 48000 0.0 41.9036 20-21 TGAGTAC 90405 0.0 41.8813 18-19 >>END_MODULE