FastQCFastQC Report
Thu 26 May 2016
SRR2132664_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132664_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4353986
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3171447.283992185551355No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA641161.4725816757334542No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG157710.36221981421162125No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT126890.29143410199297837No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT72560.16665189093396257No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT58970.1354391125740873No Hit
CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT44950.10323873342725494No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT31450.085.893961
AACGAGT840800.077.656951
ACGAGTG894450.076.299752
CGAGTGG923000.075.005413
GTGGAAT962750.074.417476
GAATCAC972100.074.233479
TGGAATC970550.074.201917
GGAATCA977450.073.750228
GAGTGGA957300.073.299744
AGTGGAA957200.073.273045
ATCGAGT13550.059.3920331
CGTGTGG24950.057.0695723
AACGTGT18900.056.7733421
ACGTGTG23050.054.277832
TCGAGTG16600.054.1174162
AGTACTT475600.041.59850720-21
GAGTACT470050.041.2748720-21
GTACTTT465650.040.48963522-23
CATGGGG243800.040.0653424-25
CGATTGG8500.038.700063
Produced by FastQC (version 0.11.5)