##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132664_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4353986
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.164531764686426	31.0	28.0	34.0	16.0	34.0
2	30.286902851777658	33.0	30.0	34.0	19.0	34.0
3	30.73710296725805	33.0	31.0	34.0	25.0	34.0
4	35.19931667212526	37.0	35.0	37.0	32.0	37.0
5	34.66087787144929	37.0	35.0	37.0	30.0	37.0
6	35.20895473710756	37.0	35.0	37.0	32.0	37.0
7	35.61606789732443	37.0	35.0	37.0	33.0	37.0
8	35.831029773637304	37.0	35.0	37.0	35.0	37.0
9	37.74925619880266	39.0	38.0	39.0	35.0	39.0
10-11	37.84882829205239	39.0	39.0	39.0	35.0	39.0
12-13	37.86514311254101	39.0	39.0	39.0	35.0	39.0
14-15	39.29415000415711	41.0	39.0	41.0	36.0	41.0
16-17	39.25477252338432	41.0	39.0	41.0	36.0	41.0
18-19	39.20850939805502	41.0	39.0	41.0	36.0	41.0
20-21	39.07744156733623	41.0	39.0	41.0	35.5	41.0
22-23	38.99275146957294	40.5	39.0	41.0	35.0	41.0
24-25	38.94906115913096	41.0	39.0	41.0	35.0	41.0
26-27	38.879601588062066	41.0	39.0	41.0	35.0	41.0
28-29	38.68182614735096	41.0	39.0	41.0	35.0	41.0
30-31	38.55592346874795	41.0	39.0	41.0	35.0	41.0
32-33	38.3967974403225	40.0	38.0	41.0	34.5	41.0
34-35	38.30915188978559	40.0	38.0	41.0	34.0	41.0
36-37	38.162918530284664	40.0	38.0	41.0	34.0	41.0
38-39	37.71447852611377	40.0	36.5	41.0	34.0	41.0
40-41	37.50224104992529	40.0	35.0	41.0	33.0	41.0
42-43	37.36030800282775	40.0	35.0	41.0	33.0	41.0
44-45	37.215870239362275	40.0	35.0	41.0	33.0	41.0
46-47	37.07245625043351	40.0	35.0	41.0	33.0	41.0
48-49	36.90698167150744	40.0	35.0	41.0	33.0	41.0
50-51	36.63098870781854	39.5	35.0	41.0	31.5	41.0
52-53	36.134951398557554	39.0	35.0	41.0	30.5	41.0
54-55	35.30262166667509	39.0	35.0	41.0	26.5	41.0
56-57	34.61385486770054	39.0	35.0	41.0	22.0	41.0
58-59	34.261163219174335	38.5	35.0	41.0	19.5	41.0
60-61	34.0054086071935	38.0	34.5	40.0	18.0	41.0
62-63	33.68994893414907	37.5	34.0	40.0	16.0	41.0
64-65	33.4055421859418	37.0	34.0	40.0	14.0	41.0
66-67	33.09174306026708	36.0	34.0	39.5	11.0	41.0
68-69	32.779193364425154	36.0	34.0	39.0	9.0	41.0
70-71	32.426648592806686	35.0	33.0	39.0	7.0	41.0
72-73	32.072013322964295	35.0	33.0	38.5	3.5	40.0
74-75	31.70772976761983	35.0	33.0	37.0	2.0	40.0
76-77	31.33387073362202	35.0	33.0	37.0	2.0	39.0
78-79	31.21461127803351	35.0	33.0	36.5	2.0	39.0
80-81	31.067025594478256	35.0	33.0	36.0	2.0	39.0
82-83	30.765716862663318	35.0	33.0	36.0	2.0	37.0
84-85	30.498883781436135	35.0	33.0	35.5	2.0	37.0
86-87	30.30341565177288	35.0	33.0	35.0	2.0	37.0
88-89	30.091911641424662	35.0	33.0	35.0	2.0	36.0
90-91	29.897489220222575	35.0	33.0	35.0	2.0	36.0
92-93	29.73109869898525	35.0	33.0	35.0	2.0	36.0
94-95	29.556075398496915	35.0	33.0	35.0	2.0	36.0
96-97	29.38088994773984	35.0	32.0	35.0	2.0	35.0
98-99	29.220607163183345	35.0	32.0	35.0	2.0	35.0
100	29.101484478820097	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	2.818997071408173
1101	2	2.3178199068162115
1101	3	2.0888389399917493
1101	4	1.0722646922424062
1101	5	1.6405182884603065
1101	6	1.0883219109520397
1101	7	0.6358217485238953
1101	8	0.482848036289127
1101	9	0.4491097229912384
1101	10-11	0.43265649749203305
1101	12-13	0.3318336404438327
1101	14-15	0.482124156279923
1101	16-17	0.4092794917806657
1101	18-19	0.43556635900952756
1101	20-21	0.5072947637540111
1101	22-23	0.4622321667453093
1101	24-25	0.5377615030266156
1101	26-27	0.4490288743759052
1101	28-29	0.4618316146329846
1101	30-31	0.48857984053504566
1101	32-33	0.47613945281607073
1101	34-35	0.5146634737228837
1101	36-37	0.5233041866695842
1101	38-39	0.49377197273825146
1101	40-41	0.48566288485010745
1101	42-43	0.4538835211362411
1101	44-45	0.5310878950132576
1101	46-47	0.5321220671826339
1101	48-49	0.4315523878101928
1101	50-51	0.513970949360619
1101	52-53	0.5521561900717487
1101	54-55	0.57731122017465
1101	56-57	0.5659094671626121
1101	58-59	0.4284880523695662
1101	60-61	0.4739473128779039
1101	62-63	0.4479946056269597
1101	64-65	0.4899686934258085
1101	66-67	0.48122057598560986
1101	68-69	0.442001914472705
1101	70-71	0.477080545019696
1101	72-73	0.5023414563524682
1101	74-75	0.42279125177552146
1101	76-77	0.3052928811244584
1101	78-79	0.40865275337607443
1101	80-81	0.40039376924620385
1101	82-83	0.4583172329264684
1101	84-85	0.4415120490176996
1101	86-87	0.4483292560793295
1101	88-89	0.44585304865482556
1101	90-91	0.35757794454448444
1101	92-93	0.4551027772566414
1101	94-95	0.4941159037102416
1101	96-97	0.5110663289704007
1101	98-99	0.5593949967635545
1101	100	0.548373777868207
1102	1	2.7246527585590243
1102	2	2.197613736271535
1102	3	2.069887387737527
1102	4	1.0377116007389517
1102	5	1.5741754122993967
1102	6	1.0566281409447456
1102	7	0.5324163878489259
1102	8	0.44100600838007864
1102	9	0.37581406835690956
1102	10-11	0.30413894472935965
1102	12-13	0.2621541070555935
1102	14-15	0.34998247117469816
1102	16-17	0.32558513767437347
1102	18-19	0.35706709430020567
1102	20-21	0.3939896195255912
1102	22-23	0.37264386173262665
1102	24-25	0.3589071737234306
1102	26-27	0.2988733382271178
1102	28-29	0.3967897476474249
1102	30-31	0.3661342722617533
1102	32-33	0.36426059187643034
1102	34-35	0.3412878574432199
1102	36-37	0.3211383606703109
1102	38-39	0.2965573124696519
1102	40-41	0.285139250669765
1102	42-43	0.2755314733601466
1102	44-45	0.24114011219322862
1102	46-47	0.19924351763288684
1102	48-49	0.22753752727366106
1102	50-51	0.17291304662490603
1102	52-53	0.01215397682859276
1102	54-55	0.03116995913610765
1102	56-57	0.04554310313051957
1102	58-59	0.028760386986519393
1102	60-61	0.009748985907705787
1102	62-63	0.0401391496033483
1102	64-65	0.09099165356008143
1102	66-67	0.00736935181186027
1102	68-69	0.09266233931015933
1102	70-71	0.09692491264294034
1102	72-73	0.1382190736286404
1102	74-75	0.16922437033088755
1102	76-77	0.08045957473969523
1102	78-79	0.09341361906558276
1102	80-81	0.1469056951853709
1102	82-83	0.2127775412380224
1102	84-85	0.14909204894091133
1102	86-87	0.19783164374159767
1102	88-89	0.21831216942798193
1102	90-91	0.18889114243719618
1102	92-93	0.21773073022856693
1102	94-95	0.242406577480903
1102	96-97	0.2512071104808271
1102	98-99	0.20001184123626814
1102	100	0.2333180455233972
1103	1	2.6464529499489515
1103	2	2.191794482084198
1103	3	2.037154745657528
1103	4	0.9934232145118997
1103	5	1.5890421008988938
1103	6	1.0724599430147492
1103	7	0.5547933201637392
1103	8	0.45464357312994963
1103	9	0.4141437586253929
1103	10-11	0.2782692587886402
1103	12-13	0.20322245810879735
1103	14-15	0.37916608089238224
1103	16-17	0.3200118067858071
1103	18-19	0.3543729873196497
1103	20-21	0.3862245379972009
1103	22-23	0.32320846953435023
1103	24-25	0.40192554663126145
1103	26-27	0.35831506160729987
1103	28-29	0.37369803549953673
1103	30-31	0.3418739142060616
1103	32-33	0.3647237712726792
1103	34-35	0.4073417810587827
1103	36-37	0.33999227692905265
1103	38-39	0.3933482702994979
1103	40-41	0.3908652496537073
1103	42-43	0.4506250147663735
1103	44-45	0.39370214829832406
1103	46-47	0.43593637639376936
1103	48-49	0.3379410188355578
1103	50-51	0.3165015918681249
1103	52-53	0.41985431552383545
1103	54-55	0.4441014521258211
1103	56-57	0.4221506011941969
1103	58-59	0.37250337853612336
1103	60-61	0.4441205661358012
1103	62-63	0.3650675895126767
1103	64-65	0.3249998594174244
1103	66-67	0.39922634648699074
1103	68-69	0.34735934998261087
1103	70-71	0.3414535356469983
1103	72-73	0.36669835695424524
1103	74-75	0.336043295338051
1103	76-77	0.37681242172590146
1103	78-79	0.3353331279452867
1103	80-81	0.34939499354553405
1103	82-83	0.4787631186799075
1103	84-85	0.4960470103748449
1103	86-87	0.5377625778381905
1103	88-89	0.5368496298123517
1103	90-91	0.4837195136390733
1103	92-93	0.48777004229831533
1103	94-95	0.5237582674569943
1103	96-97	0.4981303127845642
1103	98-99	0.4920342517622238
1103	100	0.4776342450351052
1104	1	1.4884463783834079
1104	2	1.721514129852956
1104	3	1.8570398838345703
1104	4	0.9027609498229054
1104	5	1.561610342324144
1104	6	1.054495850387731
1104	7	0.537658249626574
1104	8	0.4206318510752567
1104	9	0.3413112196270305
1104	10-11	0.26235175168964986
1104	12-13	0.2220423640592486
1104	14-15	0.3544642896861845
1104	16-17	0.3516477808310796
1104	18-19	0.3656182310769722
1104	20-21	0.3913984865850466
1104	22-23	0.2813453642487218
1104	24-25	0.3199035232331582
1104	26-27	0.31906636082238293
1104	28-29	0.46990562913010336
1104	30-31	0.44023808803743947
1104	32-33	0.3813540986204771
1104	34-35	0.27329245999519713
1104	36-37	0.31014844015421517
1104	38-39	0.3215927662756073
1104	40-41	0.26864163386134976
1104	42-43	0.31995545117777624
1104	44-45	0.3030646666414256
1104	46-47	0.3388777617387859
1104	48-49	0.3152194512261701
1104	50-51	0.2970533470544936
1104	52-53	0.29543628318887016
1104	54-55	0.3076138549608203
1104	56-57	0.33792749767578556
1104	58-59	0.31112730957959656
1104	60-61	0.3032766322370364
1104	62-63	0.23963993387614835
1104	64-65	0.2964871407471037
1104	66-67	0.23694919751969934
1104	68-69	0.21173728766191147
1104	70-71	0.218690535189495
1104	72-73	0.20149525840886895
1104	74-75	0.26399742363437895
1104	76-77	0.29512047362805305
1104	78-79	0.30822295545538125
1104	80-81	0.31478756590194834
1104	82-83	0.39735282084171786
1104	84-85	0.35238538721494805
1104	86-87	0.3293088893379874
1104	88-89	0.3767234928508145
1104	90-91	0.28037510051040115
1104	92-93	0.32301185166595303
1104	94-95	0.33789050168338974
1104	96-97	0.36196854631210584
1104	98-99	0.31363809093230444
1104	100	0.3153717468105377
1105	1	2.2170296016388775
1105	2	2.010980073888774
1105	3	2.008268152461799
1105	4	1.027044301657753
1105	5	1.628574140149155
1105	6	1.1355627042834016
1105	7	0.6126233813879338
1105	8	0.44764581910365564
1105	9	0.40083997393219306
1105	10-11	0.3626372513166274
1105	12-13	0.2968931132387951
1105	14-15	0.3621793596879783
1105	16-17	0.3153009584354223
1105	18-19	0.33269454024799927
1105	20-21	0.4269123455019539
1105	22-23	0.40789343849232296
1105	24-25	0.45675960288701134
1105	26-27	0.32643358302645
1105	28-29	0.3558770008039218
1105	30-31	0.4188604491118042
1105	32-33	0.4264991993386431
1105	34-35	0.416300776364416
1105	36-37	0.40576555790291025
1105	38-39	0.42150041683628814
1105	40-41	0.37304591672472753
1105	42-43	0.3878988365437621
1105	44-45	0.4593742189399137
1105	46-47	0.48475029877958775
1105	48-49	0.388184425206326
1105	50-51	0.4102355706307961
1105	52-53	0.45424652687824363
1105	54-55	0.34404769421306014
1105	56-57	0.29694589657968606
1105	58-59	0.24508480986159498
1105	60-61	0.29272571758049537
1105	62-63	0.23966874915021208
1105	64-65	0.14588094820413744
1105	66-67	0.1632720118723725
1105	68-69	0.193293623455439
1105	70-71	0.2206966668689745
1105	72-73	0.2128617419579868
1105	74-75	0.24339661111392985
1105	76-77	0.1963136016771614
1105	78-79	0.20520852638259868
1105	80-81	0.23956937805236223
1105	82-83	0.3088727984360844
1105	84-85	0.2878895561174346
1105	86-87	0.24891805231401776
1105	88-89	0.2276062066030491
1105	90-91	0.27881521024739797
1105	92-93	0.2580302460015744
1105	94-95	0.2947975668020426
1105	96-97	0.3345644739407909
1105	98-99	0.3197207053772608
1105	100	0.28398699367485847
1106	1	1.6424177256343881
1106	2	1.112564353253127
1106	3	1.5399218705813702
1106	4	0.777108704827171
1106	5	1.4937783708990864
1106	6	0.961435921615255
1106	7	0.5269086820668747
1106	8	0.41723873016552204
1106	9	0.33510925853158113
1106	10-11	0.3095501133780374
1106	12-13	0.25637651746637147
1106	14-15	0.329908117472435
1106	16-17	0.3116582546947271
1106	18-19	0.3105712473180162
1106	20-21	0.3245867005159013
1106	22-23	0.24705405051759755
1106	24-25	0.3430852959127222
1106	26-27	0.30088231720285563
1106	28-29	0.3201820106112976
1106	30-31	0.30215823881562187
1106	32-33	0.3341116732653262
1106	34-35	0.31606124289814375
1106	36-37	0.27407495218713507
1106	38-39	0.28765138275515767
1106	40-41	0.2862252007364887
1106	42-43	0.27046356033501695
1106	44-45	0.23733487746071802
1106	46-47	0.32646973043609506
1106	48-49	0.29782596355634183
1106	50-51	0.18981159489425892
1106	52-53	0.22804422715108075
1106	54-55	0.2620869619170634
1106	56-57	0.1974173929764973
1106	58-59	0.12450509126249187
1106	60-61	0.18691087058038391
1106	62-63	0.2735054408990152
1106	64-65	0.1710504274070388
1106	66-67	0.23390794116313884
1106	68-69	0.2173010468697143
1106	70-71	0.19791771836276695
1106	72-73	0.18765416344423613
1106	74-75	0.22880571171283748
1106	76-77	0.19987227890511505
1106	78-79	0.20958204417678772
1106	80-81	0.2446272143718069
1106	82-83	0.2604898369883415
1106	84-85	0.2250420460087348
1106	86-87	0.2071894094815967
1106	88-89	0.14477305048744782
1106	90-91	0.20813854375373353
1106	92-93	0.18373203251933035
1106	94-95	0.19523858803943384
1106	96-97	0.16924184901944272
1106	98-99	0.19050133269801606
1106	100	0.1900742405605378
1107	1	1.6301930882376112
1107	2	1.096254709026482
1107	3	1.4414474605303091
1107	4	0.775960561508434
1107	5	1.4433867876909758
1107	6	0.9805395233199121
1107	7	0.525651771495717
1107	8	0.4285804222252807
1107	9	0.3701176919497726
1107	10-11	0.230581321878212
1107	12-13	0.18574198767216643
1107	14-15	0.2875381244056925
1107	16-17	0.25792633527237285
1107	18-19	0.26512280341765404
1107	20-21	0.24234819239327265
1107	22-23	0.2591884142597678
1107	24-25	0.28653145179740136
1107	26-27	0.20382919236505614
1107	28-29	0.2760067765454366
1107	30-31	0.20038635592049303
1107	32-33	0.2201201007757021
1107	34-35	0.14630823423348005
1107	36-37	0.17783072444728276
1107	38-39	0.18999549111477165
1107	40-41	0.23574030556448378
1107	42-43	0.16522110536423895
1107	44-45	0.2045524275791024
1107	46-47	0.2769149482427835
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2214	4	-1.4192294364200748
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2214	96-97	-0.4914255744083178
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2214	100	-0.484479125331692
2215	1	-5.38830540520625
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2215	3	-3.4711085113655784
2215	4	-1.8074230066728134
2215	5	-2.632013761826066
2215	6	-5.095107388982111
2215	7	-2.2258305642681364
2215	8	-1.1938430438422216
2215	9	-1.566496042192405
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2215	24-25	-0.8094885863615104
2215	26-27	-0.8487301181910212
2215	28-29	-0.9034370339830673
2215	30-31	-1.0742860492620423
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2216	1	-5.808311098200029
2216	2	-4.787358838223401
2216	3	-5.762733967790339
2216	4	-3.0375457158150425
2216	5	-4.234137430929582
2216	6	-1.9945599632289586
2216	7	-1.2887080714527883
2216	8	-0.8929186293489053
2216	9	-0.7458647136201009
2216	10-11	-1.1016065361584708
2216	12-13	-0.8978760142650088
2216	14-15	-1.8260431739014749
2216	16-17	-1.296640260629907
2216	18-19	-1.8720657184202594
2216	20-21	-1.3018784888594084
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2216	24-25	-1.667892167130688
2216	26-27	-1.3960960243519622
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2216	30-31	-2.1837151820734917
2216	32-33	-1.3420134769843912
2216	34-35	-1.3007251597223402
2216	36-37	-1.3172838217618263
2216	38-39	-1.2225744699252061
2216	40-41	-1.2207380818950568
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2216	52-53	-1.2946235265602795
2216	54-55	-1.1306491224378377
2216	56-57	-0.9940724007291664
2216	58-59	-0.9251698083131501
2216	60-61	-0.962491848530405
2216	62-63	-0.8975609330195766
2216	64-65	-0.9907402375299057
2216	66-67	-1.0465921001353564
2216	68-69	-1.0156051939675343
2216	70-71	-0.9570255001013734
2216	72-73	-0.9445876280065946
2216	74-75	-0.8326187546738595
2216	76-77	-0.8672123300248415
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2216	80-81	-0.8374453089039626
2216	82-83	-0.8266326240725164
2216	84-85	-0.8612390277699724
2216	86-87	-0.882997845245967
2216	88-89	-0.98132520564738
2216	90-91	-0.922220848893442
2216	92-93	-0.8755796206249578
2216	94-95	-0.8930294888465653
2216	96-97	-0.9174104325610486
2216	98-99	-0.9689239636660005
2216	100	-1.0125356908038121
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	6505.0
3	3274.0
4	3692.0
5	4701.0
6	4347.0
7	3560.0
8	3855.0
9	4420.0
10	5125.0
11	11406.0
12	7879.0
13	11353.0
14	10545.0
15	10571.0
16	12552.0
17	15660.0
18	21223.0
19	35609.0
20	74354.0
21	99211.0
22	46536.0
23	30627.0
24	32045.0
25	34626.0
26	35255.0
27	37994.0
28	43053.0
29	50048.0
30	58410.0
31	71338.0
32	91767.0
33	123234.0
34	178230.0
35	326432.0
36	516176.0
37	884763.0
38	1084271.0
39	356940.0
40	2399.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	14.782075335546255	41.58850046835372	25.603390347776767	18.026033848323255
2	18.102946212488593	39.076559235671716	25.165076614395275	17.65541793744441
3	19.129207121933785	24.013421265020142	20.50580778165111	36.351563831394955
4	33.983503851413396	25.017099274090455	22.2269892461758	18.772407628320348
5	17.995854832790002	38.49449676687063	23.371940102701295	20.13770829763807
6	36.86727518186783	22.190723626580333	23.554439541146895	17.38756165040494
7	19.33136670627788	22.69396364618536	39.36758179746099	18.60708785007577
8	35.69832332947327	22.615943184015748	23.278485507302964	18.40724797920802
9	35.41851076232216	24.292797450428182	22.96068476104425	17.328007026205412
10-11	17.31929087507401	41.80803521187252	22.9882457132384	17.884428199815066
12-13	17.33874431383105	24.00796649323172	31.53379455055666	27.11949464238057
14-15	17.6708308203104	32.585451583904955	22.79025472291367	26.953462872870976
16-17	26.749289099199515	23.673163170366077	31.560630806321477	18.016916924112934
18-19	17.895877478705717	23.87006986242032	31.655384284653188	26.578668374220772
20-21	26.90766746387032	32.87366079087839	22.65590315411065	17.562768591140635
22-23	26.462690121982618	24.14527073039593	31.654111490151198	17.737927657470255
24-25	17.688148285272394	32.79870445150719	22.723488316223342	26.789658946997076
26-27	18.044538062274	27.80072861589967	36.49394567570494	17.66078764612139
28-29	26.21852304315701	23.89803807540712	32.32878320068334	17.554655680752532
30-31	24.17611002449284	25.069426236841284	32.604611939463744	18.14985179920213
32-33	20.307809372616017	26.537723345709512	33.72616247602596	19.42830480564851
34-35	19.941252454130613	26.28705771674211	34.09746332240363	19.674226506723652
36-37	19.95884128536743	26.467966017817986	34.14700981444354	19.42618288237104
38-39	20.436621740353857	26.407575644463844	33.78869510787483	19.367107507307466
40-41	19.651405198362877	27.06240669898824	33.81880387973729	19.467384222911598
42-43	19.850791521577346	26.58047059472074	34.04156062513207	19.527177258569843
44-45	19.722770644460695	26.77624495165718	33.732989978649044	19.76799442523308
46-47	19.978145992350523	26.629757165570812	33.89911441313721	19.492982428941456
48-49	19.633042505004504	26.87416373887947	33.779189970390426	19.713603785725596
50-51	20.028985437338658	27.10573666572835	33.21925442490758	19.6460234720254
52-53	20.187207413052498	27.097404264838758	32.91943376803066	19.795954554078087
54-55	20.480113387762582	27.792335577064197	31.47169589442499	20.25585514074823
56-57	20.836010971156846	28.494495358480393	30.345257951263697	20.324235719099065
58-59	21.422808183656848	28.934281683787532	29.336445605894056	20.30646452666156
60-61	21.073752788724423	29.40355463740783	28.97502189520483	20.547670678662918
62-63	20.57270295814227	29.40977258398476	29.004263978511297	21.01326047936168
64-65	20.77729866279373	29.286716897558335	29.413656974445917	20.52232746520202
66-67	20.680618891872168	28.856319353314547	29.874655537595647	20.588406217217642
68-69	20.843465097956344	28.766156652049972	29.908394912564003	20.481983337429682
70-71	20.22816599177543	29.259848477365896	29.979940466852156	20.532045064006518
72-73	20.283918214686107	28.860275497962807	30.09896667018085	20.75683961717024
74-75	20.434716550894837	29.091138979136954	29.59349382489631	20.8806506450719
76-77	20.791710914681254	28.87789178743451	29.627827446696525	20.70256985118771
78-79	20.678089523713265	29.326389877930563	29.084674473842593	20.910846124513583
80-81	21.00172684992071	29.685671050479694	28.744207082335688	20.568395017263903
82-83	20.87400310700208	29.77137109013464	28.757501209599294	20.59712459326398
84-85	20.849223310437065	29.989975346633774	28.235081049936877	20.925720292992285
86-87	20.82109123191248	30.068143853759743	28.31646724621769	20.79429766811009
88-89	21.417221700836926	29.79768642739905	28.039239870769944	20.745852000994077
90-91	21.1334137780509	30.266798615297354	27.863310743044817	20.736476863606924
92-93	20.838283864012993	30.419919469198213	27.712700352211716	21.029096314577075
94-95	20.979496030407965	30.578549318770047	27.54895033898414	20.893004311837846
96-97	20.94169835001571	30.172578543772637	27.726854463631067	21.158868642580583
98-99	21.3293339882282	30.288160248807987	27.1304362006344	21.25206956232941
100	20.926604674365866	30.795654628498664	27.112589845273426	21.165150851862045
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	59967.0
1	33609.5
2	5751.0
3	3758.0
4	3190.0
5	3014.0
6	3036.5
7	3325.5
8	3254.0
9	3179.0
10	3416.0
11	4817.5
12	38349.0
13	44047.0
14	15953.5
15	15212.0
16	16931.0
17	18716.5
18	20398.0
19	22072.0
20	23884.5
21	26390.0
22	29529.5
23	33289.5
24	37485.5
25	41942.5
26	47577.0
27	53592.0
28	58719.0
29	63467.0
30	68548.0
31	73160.0
32	78931.0
33	85998.5
34	92811.0
35	97855.0
36	102775.5
37	106913.0
38	109067.5
39	112007.0
40	114844.5
41	117397.0
42	118821.0
43	120633.0
44	123678.0
45	125889.5
46	126997.0
47	129531.5
48	131597.0
49	136977.5
50	157299.5
51	155803.0
52	131412.0
53	120816.0
54	117316.5
55	114847.0
56	107146.5
57	97113.0
58	89944.0
59	78803.5
60	65137.5
61	54024.5
62	43355.5
63	33689.5
64	25768.5
65	19352.0
66	14460.0
67	10705.0
68	8103.0
69	6209.0
70	4657.5
71	3800.0
72	3279.0
73	2452.5
74	1799.0
75	1408.0
76	1053.0
77	728.0
78	497.0
79	315.0
80	151.5
81	80.5
82	52.0
83	29.0
84	17.0
85	14.0
86	7.5
87	4.0
88	4.0
89	4.0
90	3.0
91	0.5
92	0.0
93	0.5
94	0.5
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.059118242456452544
2	0.06835116144149292
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	6.890238048537592E-5
18-19	0.0
20-21	3.904468227504636E-4
22-23	1.1483730080895988E-4
24-25	0.0
26-27	0.0026297741885251812
28-29	0.0010794706276042228
30-31	0.006074893212793977
32-33	0.0013206289593030386
34-35	0.0023426809365027818
36-37	0.00807306224686988
38-39	0.012884745150765299
40-41	0.003089123391761021
42-43	0.007946741215980024
44-45	0.001883331733266942
46-47	0.00214745752512755
48-49	0.0036173749754822362
50-51	0.009657816998033527
52-53	0.0034910539445923805
54-55	0.0017684944324579822
56-57	5.512190438830074E-4
58-59	9.18698406471679E-4
60-61	0.006729465827405049
62-63	0.0016306896714872305
64-65	0.008199383277759736
66-67	0.010633934054909686
68-69	0.01185120944348466
70-71	0.010220519771997429
72-73	0.00452458965187302
74-75	0.002101522604803966
76-77	0.004880585284380795
78-79	0.016674376077460973
80-81	0.00904917930374604
82-83	0.006235665433926521
84-85	0.0050413575055133384
86-87	0.007544810663148664
88-89	0.004593492032358396
90-91	0.006235665433926521
92-93	0.011403343970329717
94-95	0.00895730946309887
96-97	0.01125405547927807
98-99	0.0024460345072308456
100	0.0025723555381207013
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4353986.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.770391749343105
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.89002448106005	46.15278011135451
2	13.302895905574918	15.370270157315913
3	3.3826641062194076	5.862534917182102
4	1.1635785207679807	2.688815479035497
5	0.5375407509051476	1.5526969880513224
6	0.3306599205924821	1.1461411889060626
7	0.2255968114946594	0.9122971324214431
8	0.1696875269574146	0.7842331925845649
9	0.12990316667313404	0.675410114536845
>10	0.8049306174058383	9.582839032758642
>50	0.0460054688589778	1.668519817423285
>100	0.012467982626344722	1.3856780798461243
>500	0.002162534807599118	0.8709968007950052
>1k	0.0016419244320135084	1.5635048575845145
>5k	8.009387473236493E-5	0.30429824050855864
>10k+	1.6018774946472986E-4	9.478983889695632
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	317144	7.283992185551355	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	64116	1.4725816757334542	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	15771	0.36221981421162125	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	12689	0.29143410199297837	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7256	0.16665189093396257	No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5897	0.1354391125740873	No Hit
CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4495	0.10323873342725494	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.003812598386857468	0.0	0.0	0.0	0.0
2	0.003812598386857468	0.0	0.0	0.0	0.0
3	0.003812598386857468	4.5934920323583954E-5	0.0	0.0	0.0
4	0.003812598386857468	4.5934920323583954E-5	0.0	2.2967460161791977E-5	0.0
5	0.003812598386857468	4.5934920323583954E-5	0.0	2.2967460161791977E-5	0.0
6	0.003812598386857468	4.5934920323583954E-5	0.0	2.2967460161791977E-5	0.0
7	0.003812598386857468	9.186984064716791E-5	0.0	2.2967460161791977E-5	2.2967460161791977E-5
8	0.003812598386857468	9.186984064716791E-5	0.0	2.2967460161791977E-5	2.2967460161791977E-5
9	0.003812598386857468	9.186984064716791E-5	0.0	2.2967460161791977E-5	2.2967460161791977E-5
10-11	0.003812598386857468	9.186984064716791E-5	0.0	2.2967460161791977E-5	2.2967460161791977E-5
12-13	0.003824082116938364	9.186984064716791E-5	0.0	2.2967460161791977E-5	2.2967460161791977E-5
14-15	0.0038355658470192603	9.186984064716791E-5	0.0	2.2967460161791977E-5	2.2967460161791977E-5
16-17	0.003881500767342844	1.0335357072806389E-4	0.0	2.2967460161791977E-5	2.2967460161791977E-5
18-19	0.003927435687666428	1.2632103088985587E-4	0.0	2.2967460161791977E-5	2.2967460161791977E-5
20-21	0.003973370607990012	1.3780476097075185E-4	0.0	2.2967460161791977E-5	2.2967460161791977E-5
22-23	0.004076724178718075	1.8373968129433582E-4	0.0	2.2967460161791977E-5	2.2967460161791977E-5
24-25	0.004168594019365243	2.641257918606077E-4	0.0	2.2967460161791977E-5	2.2967460161791977E-5
26-27	0.004203045209607932	3.2154444226508767E-4	0.0	2.2967460161791977E-5	2.2967460161791977E-5
28-29	0.004283431320174204	3.445119024268796E-4	0.0	2.2967460161791977E-5	2.2967460161791977E-5
30-31	0.004432719811225851	3.6747936258867164E-4	0.0	2.2967460161791977E-5	2.2967460161791977E-5
32-33	0.004582008302277499	4.0193055283135957E-4	0.0	2.2967460161791977E-5	2.2967460161791977E-5
34-35	0.004846134094138107	4.2489801299315153E-4	0.0	2.2967460161791977E-5	4.5934920323583954E-5
36-37	0.005443288058344698	4.593492032358395E-4	0.0	2.2967460161791977E-5	6.890238048537592E-5
38-39	0.006235665433926521	4.7083293331673555E-4	0.0	2.2967460161791977E-5	6.890238048537592E-5
40-41	0.007407005902177913	5.052841235594235E-4	0.0	2.2967460161791977E-5	8.038611056627192E-5
42-43	0.008865439622451704	5.052841235594235E-4	0.0	2.2967460161791977E-5	9.186984064716791E-5
44-45	0.010174584851673846	5.167678536403195E-4	0.0	2.2967460161791977E-5	9.186984064716791E-5
46-47	0.011782307062999285	5.282515837212154E-4	0.0	2.2967460161791977E-5	9.186984064716791E-5
48-49	0.01388382966780325	5.397353138021114E-4	0.0	2.2967460161791977E-5	9.186984064716791E-5
50-51	0.017122241550615917	5.627027739639034E-4	0.0	6.890238048537592E-5	9.186984064716791E-5
52-53	0.021474575251275496	5.856702341256953E-4	0.0	6.890238048537592E-5	9.186984064716791E-5
54-55	0.026355160535656293	5.971539642065914E-4	0.0	6.890238048537592E-5	9.186984064716791E-5
56-57	0.032453021208612064	6.430888845301753E-4	0.0	6.890238048537592E-5	9.186984064716791E-5
58-59	0.0397911247303046	6.890238048537592E-4	0.0	6.890238048537592E-5	9.186984064716791E-5
60-61	0.049138881016153935	7.119912650155513E-4	0.0	6.890238048537592E-5	9.186984064716791E-5
62-63	0.06195472378643385	7.349587251773433E-4	0.0	6.890238048537592E-5	9.186984064716791E-5
64-65	0.07742330820540075	7.349587251773433E-4	0.0	6.890238048537592E-5	9.186984064716791E-5
66-67	0.09701455172340931	7.694099154200312E-4	0.0	6.890238048537592E-5	9.186984064716791E-5
68-69	0.12156676663636493	8.268285658245111E-4	0.0	6.890238048537592E-5	9.186984064716791E-5
70-71	0.15335373150028503	8.842472162289911E-4	0.0	6.890238048537592E-5	9.186984064716791E-5
72-73	0.19328266099156038	8.957309463098871E-4	0.0	6.890238048537592E-5	9.186984064716791E-5
74-75	0.24284644002070746	9.41665866633471E-4	0.0	6.890238048537592E-5	9.186984064716791E-5
76-77	0.30550167134207595	9.646333267952631E-4	0.0	6.890238048537592E-5	9.186984064716791E-5
78-79	0.38281014224666776	9.87600786957055E-4	0.0	6.890238048537592E-5	9.186984064716791E-5
80-81	0.4799969499212905	0.001010568247118847	0.0	6.890238048537592E-5	9.186984064716791E-5
82-83	0.6006565018812646	0.001033535707280639	0.0	6.890238048537592E-5	9.186984064716791E-5
84-85	0.7485324941329623	0.001033535707280639	0.0	8.038611056627192E-5	9.186984064716791E-5
86-87	0.9217760461333592	0.001045019437361535	0.0	9.186984064716791E-5	9.186984064716791E-5
88	1.0681706372046211	0.0010794706276042228	0.0	9.186984064716791E-5	9.186984064716791E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	3145	0.0	85.89396	1
AACGAGT	84080	0.0	77.65695	1
ACGAGTG	89445	0.0	76.29975	2
CGAGTGG	92300	0.0	75.00541	3
GTGGAAT	96275	0.0	74.41747	6
GAATCAC	97210	0.0	74.23347	9
TGGAATC	97055	0.0	74.20191	7
GGAATCA	97745	0.0	73.75022	8
GAGTGGA	95730	0.0	73.29974	4
AGTGGAA	95720	0.0	73.27304	5
ATCGAGT	1355	0.0	59.392033	1
CGTGTGG	2495	0.0	57.069572	3
AACGTGT	1890	0.0	56.773342	1
ACGTGTG	2305	0.0	54.27783	2
TCGAGTG	1660	0.0	54.117416	2
AGTACTT	47560	0.0	41.598507	20-21
GAGTACT	47005	0.0	41.27487	20-21
GTACTTT	46565	0.0	40.489635	22-23
CATGGGG	24380	0.0	40.06534	24-25
CGATTGG	850	0.0	38.70006	3
>>END_MODULE