FastQCFastQC Report
Thu 26 May 2016
SRR2132663_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132663_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6340554
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5508768.688136714867502No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA269120.4244424067676105No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT211370.3333620374497244No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA111830.1763726008799862No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT104540.16487518283102706No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT100300.15818807000145413No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT67680.10674146139280573No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT63510.10016474901089084No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGAGT1167000.092.974131
ACGAGTG1249800.090.80642
CGAGTGG1289900.089.296713
GAGTGGA1338000.087.182364
GTGGAAT1372300.086.862716
TGGAATC1387750.086.383287
GAATCAC1391000.086.316589
TACGAGT51500.086.165751
GGAATCA1394550.086.083378
AGTGGAA1356400.085.95125
ATCGAGT19350.072.714391
TCGAGTG23800.067.378772
AACGTGT28950.066.319581
CGTGTGG37500.065.413843
ACGTGTG37550.059.613232
CGATTGG11050.050.1822553
GACGAGT13850.048.5865941
ACGATTG10550.048.14122
ATCACTG1409000.043.8042710-11
TGAGTAC1392450.043.65197818-19