##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132663_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6340554
Sequences flagged as poor quality	0
Sequence length	100
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.252438982461154	31.0	28.0	34.0	16.0	34.0
2	30.370876109563927	33.0	30.0	34.0	23.0	34.0
3	30.82813079109491	33.0	31.0	34.0	25.0	34.0
4	35.2820900192633	37.0	35.0	37.0	32.0	37.0
5	34.73757403532878	37.0	35.0	37.0	32.0	37.0
6	35.30783840024073	37.0	35.0	37.0	33.0	37.0
7	35.68678667510757	37.0	35.0	37.0	35.0	37.0
8	35.92109096460656	37.0	35.0	37.0	35.0	37.0
9	37.858552580736635	39.0	39.0	39.0	35.0	39.0
10-11	37.96087084819402	39.0	39.0	39.0	35.0	39.0
12-13	37.98871746853666	39.0	39.0	39.0	36.0	39.0
14-15	39.446522496299224	41.0	40.0	41.0	37.0	41.0
16-17	39.43328682320188	41.0	39.5	41.0	37.0	41.0
18-19	39.442848527116084	41.0	39.5	41.0	37.0	41.0
20-21	39.33503972996681	41.0	39.5	41.0	36.5	41.0
22-23	39.26103720589715	40.5	39.0	41.0	36.0	41.0
24-25	39.24891484245698	41.0	39.0	41.0	36.0	41.0
26-27	39.19314566203521	41.0	39.0	41.0	36.0	41.0
28-29	39.02112812224294	41.0	39.0	41.0	36.0	41.0
30-31	38.90226587455923	41.0	39.0	41.0	35.0	41.0
32-33	38.74168219370105	41.0	39.0	41.0	35.0	41.0
34-35	38.67695732896526	41.0	39.0	41.0	35.0	41.0
36-37	38.546620847326594	40.5	38.5	41.0	35.0	41.0
38-39	38.04744482895343	40.0	36.5	41.0	34.5	41.0
40-41	37.82303107898774	40.0	36.0	41.0	34.0	41.0
42-43	37.687324719574974	40.0	36.0	41.0	33.0	41.0
44-45	37.55533349294084	40.0	35.0	41.0	33.0	41.0
46-47	37.42807497578288	40.0	35.0	41.0	33.0	41.0
48-49	37.27859056164493	40.0	35.0	41.0	33.0	41.0
50-51	37.00691966348682	40.0	35.0	41.0	33.0	41.0
52-53	36.46389518960015	40.0	35.0	41.0	31.0	41.0
54-55	35.475824431114376	39.5	35.0	41.0	26.0	41.0
56-57	34.613824517542156	39.0	35.0	41.0	17.5	41.0
58-59	34.20710879522515	39.0	35.0	41.0	11.5	41.0
60-61	33.9358296609413	39.0	35.0	41.0	8.0	41.0
62-63	33.60538605932541	38.0	34.0	40.5	7.0	41.0
64-65	33.32621423932356	38.0	34.0	40.0	3.5	41.0
66-67	33.013175504853365	37.0	34.0	40.0	2.0	41.0
68-69	32.66828442751217	37.0	34.0	39.5	2.0	41.0
70-71	32.28216879786845	36.0	33.0	39.0	2.0	41.0
72-73	31.895282336527693	35.5	33.0	39.0	2.0	40.5
74-75	31.49958418459964	35.0	33.0	38.5	2.0	40.0
76-77	31.09203872406102	35.0	33.0	37.0	2.0	39.5
78-79	30.92539784378463	35.0	33.0	37.0	2.0	39.0
80-81	30.740540889644656	35.0	33.0	36.5	2.0	39.0
82-83	30.412449763853445	35.0	33.0	36.0	2.0	38.0
84-85	30.112556647258266	35.0	33.0	36.0	2.0	37.0
86-87	29.87360260633377	35.0	33.0	35.5	2.0	37.0
88-89	29.63030564837079	35.0	33.0	35.0	2.0	36.5
90-91	29.41377480579773	35.0	32.0	35.0	2.0	36.0
92-93	29.223238537200377	35.0	32.0	35.0	2.0	36.0
94-95	29.038705214086974	35.0	32.0	35.0	2.0	36.0
96-97	28.864799511209903	35.0	32.0	35.0	2.0	36.0
98-99	28.691098680020705	35.0	32.0	35.0	2.0	35.0
100	28.55678494339769	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	2.791627113689099
1101	2	2.2347688649653286
1101	3	2.0290065210976174
1101	4	0.996043683148244
1101	5	1.5676302773660922
1101	6	1.0262104418053752
1101	7	0.5503218596392827
1101	8	0.43754104230561097
1101	9	0.40481826711499025
1101	10-11	0.3573862440768849
1101	12-13	0.2734042976761799
1101	14-15	0.398261213091196
1101	16-17	0.3377914729997613
1101	18-19	0.3400155437089083
1101	20-21	0.37887720880002007
1101	22-23	0.337804264093009
1101	24-25	0.3821341049963749
1101	26-27	0.3502971332122726
1101	28-29	0.3515976862698409
1101	30-31	0.38633139054248034
1101	32-33	0.4102479947990503
1101	34-35	0.3883328783353335
1101	36-37	0.36078687578208246
1101	38-39	0.33603408387557465
1101	40-41	0.38039105417458785
1101	42-43	0.356429324350664
1101	44-45	0.38693369476756345
1101	46-47	0.3882633461216969
1101	48-49	0.3063974508508096
1101	50-51	0.3520697805711208
1101	52-53	0.36716633276004274
1101	54-55	0.3624081040308482
1101	56-57	0.11294150603276165
1101	58-59	0.04703828764290563
1101	60-61	-0.013561698405574418
1101	62-63	0.013946839055023474
1101	64-65	-0.06531367070849114
1101	66-67	0.005065909218934905
1101	68-69	0.04744779890132378
1101	70-71	-0.002219436890179338
1101	72-73	-0.043958411464672054
1101	74-75	0.048805179145130495
1101	76-77	-0.1120575416474594
1101	78-79	-0.08430362687904136
1101	80-81	-0.013414825313958545
1101	82-83	0.0372495286337724
1101	84-85	0.0024272883728997385
1101	86-87	0.021952707899334456
1101	88-89	-0.009453678885797956
1101	90-91	-0.09217718543872877
1101	92-93	-0.032157604260468986
1101	94-95	-0.009300579011746635
1101	96-97	0.055586000868668606
1101	98-99	-0.01013132644164827
1101	100	-0.041520126379374744
1102	1	2.769659937388166
1102	2	2.260901370402568
1102	3	2.047695604069201
1102	4	1.035390944572427
1102	5	1.5836345541194277
1102	6	1.0274742320074068
1102	7	0.5508393606961093
1102	8	0.46523295855368474
1102	9	0.43262512737861414
1102	10-11	0.3400006812388341
1102	12-13	0.28998980616826486
1102	14-15	0.39477988334314773
1102	16-17	0.35584195121965223
1102	18-19	0.38714394301592847
1102	20-21	0.37373521851259284
1102	22-23	0.3272465297708891
1102	24-25	0.3715337808916459
1102	26-27	0.3220823701856901
1102	28-29	0.4382811481820781
1102	30-31	0.39923812956601523
1102	32-33	0.350011621945562
1102	34-35	0.3285943175637627
1102	36-37	0.3234639269640809
1102	38-39	0.27305737969469845
1102	40-41	0.317386400448882
1102	42-43	0.3327510001389342
1102	44-45	0.3273810133183588
1102	46-47	0.2654862643876825
1102	48-49	0.2849455097246789
1102	50-51	0.27559057538445586
1102	52-53	0.22325510535419113
1102	54-55	0.1087299505302326
1102	56-57	0.028140013137679887
1102	58-59	0.0326371334245934
1102	60-61	0.020000535210293435
1102	62-63	-0.058332553790663155
1102	64-65	0.020941264514561908
1102	66-67	-0.07067715929394325
1102	68-69	-0.031204926123201915
1102	70-71	-0.05290816383229924
1102	72-73	-0.07547631351662076
1102	74-75	-0.031113359976050248
1102	76-77	-0.12759005296279113
1102	78-79	-0.0392638978274924
1102	80-81	0.021424584806993607
1102	82-83	0.04654749854426399
1102	84-85	0.029467180567948503
1102	86-87	0.031762811146119674
1102	88-89	0.027866773062193317
1102	90-91	0.013103921205736668
1102	92-93	0.028526049305796164
1102	94-95	0.019873906047859435
1102	96-97	-0.007967293292626465
1102	98-99	0.013791790025212691
1102	100	0.03544935628147883
1103	1	2.6470635503523567
1103	2	2.1925941162164087
1103	3	1.9976334541792404
1103	4	1.018351900502175
1103	5	1.6168036010447508
1103	6	1.0831546406287131
1103	7	0.6119590046618413
1103	8	0.4894819799987573
1103	9	0.48502356262116564
1103	10-11	0.351812369484513
1103	12-13	0.284969031529414
1103	14-15	0.41357248754931675
1103	16-17	0.40855196512283243
1103	18-19	0.396303077361182
1103	20-21	0.4570022975418624
1103	22-23	0.4138118540952789
1103	24-25	0.46975031424418745
1103	26-27	0.4243914789610912
1103	28-29	0.3997410496571874
1103	30-31	0.39669105774421354
1103	32-33	0.4280822359458085
1103	34-35	0.4230077185325527
1103	36-37	0.4386300034623787
1103	38-39	0.41609178367346544
1103	40-41	0.38042967353742796
1103	42-43	0.4149267461831414
1103	44-45	0.41416388164175544
1103	46-47	0.42762188976599447
1103	48-49	0.403589651808943
1103	50-51	0.4377352305456128
1103	52-53	0.46399107583928156
1103	54-55	0.4618596818779821
1103	56-57	0.38948568920597637
1103	58-59	0.36912593343549105
1103	60-61	0.32970056485511634
1103	62-63	0.35058746738199886
1103	64-65	0.2977257972902265
1103	66-67	0.3320366603638618
1103	68-69	0.32697742909744676
1103	70-71	0.33046842702482593
1103	72-73	0.3426548345970666
1103	74-75	0.35304017988836023
1103	76-77	0.28714299456490266
1103	78-79	0.2899253377323028
1103	80-81	0.333492759807438
1103	82-83	0.41587654723397804
1103	84-85	0.44271568225936875
1103	86-87	0.3978140002042885
1103	88-89	0.4295433953561023
1103	90-91	0.41568821167092196
1103	92-93	0.4167806020824578
1103	94-95	0.4519820218723716
1103	96-97	0.4328971757944835
1103	98-99	0.40339797260391563
1103	100	0.46149205335926524
1104	1	1.5608931850249306
1104	2	1.7216373169366257
1104	3	1.7970045392983884
1104	4	0.885323506182722
1104	5	1.5228542160355616
1104	6	1.0267578054999262
1104	7	0.5367625755702718
1104	8	0.43254780766326917
1104	9	0.3889059189099058
1104	10-11	0.3009114365215666
1104	12-13	0.24026046185596783
1104	14-15	0.34373726064680454
1104	16-17	0.2891999881327294
1104	18-19	0.3166761023788638
1104	20-21	0.3459673509137957
1104	22-23	0.24697808004364674
1104	24-25	0.3023136866344984
1104	26-27	0.25370780899479684
1104	28-29	0.3168273657769234
1104	30-31	0.3347417013155649
1104	32-33	0.26350154669779613
1104	34-35	0.20272214616335305
1104	36-37	0.23006745936038442
1104	38-39	0.23739622475184774
1104	40-41	0.28472218575718955
1104	42-43	0.2470075642329732
1104	44-45	0.2603123262461082
1104	46-47	0.35378715058462973
1104	48-49	0.29959259349942613
1104	50-51	0.2605051080340388
1104	52-53	0.2569772789333129
1104	54-55	0.24317915488659736
1104	56-57	0.23003305401331176
1104	58-59	0.24273895143558377
1104	60-61	0.15616403977335835
1104	62-63	0.1710097159520032
1104	64-65	0.17664402972683746
1104	66-67	0.2464185065384541
1104	68-69	0.12595401128471195
1104	70-71	0.11627104526279908
1104	72-73	0.04295235838625544
1104	74-75	0.12625502452558024
1104	76-77	0.15835782577474333
1104	78-79	0.08100162764045749
1104	80-81	0.15076195241532275
1104	82-83	0.18463023976331883
1104	84-85	0.2030355020226864
1104	86-87	0.14672637194756177
1104	88-89	0.15218728277523041
1104	90-91	0.12410143405341145
1104	92-93	0.07551862393398068
1104	94-95	0.0211263332852063
1104	96-97	0.08898177489334103
1104	98-99	0.13091126865599634
1104	100	0.1510127476869485
1105	1	2.1484016905775327
1105	2	1.943316696942329
1105	3	1.9026023121686144
1105	4	0.9060110305327953
1105	5	1.5167330266013082
1105	6	0.9965162350498389
1105	7	0.5054972937628364
1105	8	0.40632414380863224
1105	9	0.3454983846774198
1105	10-11	0.3022816555327523
1105	12-13	0.23320169161484472
1105	14-15	0.3153872549352883
1105	16-17	0.25036923949431866
1105	18-19	0.23864796002776245
1105	20-21	0.31161649164312166
1105	22-23	0.2920467534309168
1105	24-25	0.3619933830368822
1105	26-27	0.24582151164990051
1105	28-29	0.2818165083382169
1105	30-31	0.3055890380858983
1105	32-33	0.2871650114443227
1105	34-35	0.2554161379841986
1105	36-37	0.24466838537516367
1105	38-39	0.2914951244123358
1105	40-41	0.169191937592565
1105	42-43	0.18960525319985067
1105	44-45	0.23186225067136945
1105	46-47	0.2757161564200956
1105	48-49	0.16047110783163276
1105	50-51	0.19286757629355122
1105	52-53	0.17014528269040596
1105	54-55	0.11290038858922458
1105	56-57	0.015127140979480203
1105	58-59	0.002760252557159504
1105	60-61	0.11737577774868413
1105	62-63	0.09528562883605218
1105	64-65	0.060452692938724795
1105	66-67	0.0728861762622941
1105	68-69	0.0022549915177592084
1105	70-71	0.015532100176820052
1105	72-73	0.09660153261107851
1105	74-75	0.09359493702249821
1105	76-77	0.05310523863830241
1105	78-79	0.04845272544517698
1105	80-81	0.07335681504509495
1105	82-83	0.1104844737673254
1105	84-85	0.11347673959993188
1105	86-87	0.06607397137364401
1105	88-89	0.08744871659165909
1105	90-91	0.14151440809497728
1105	92-93	0.13267590139506424
1105	94-95	0.17854987425168645
1105	96-97	0.1434457302378327
1105	98-99	0.14904981221569713
1105	100	0.14427538919309058
1106	1	1.5680356751663567
1106	2	1.032993144093627
1106	3	1.5264919575717535
1106	4	0.7467589726507171
1106	5	1.4361035641492634
1106	6	0.9347790576354171
1106	7	0.4728279440305343
1106	8	0.36324522966170036
1106	9	0.351742619787224
1106	10-11	0.26531041662457966
1106	12-13	0.212959190838248
1106	14-15	0.28773870250368816
1106	16-17	0.2782162660225467
1106	18-19	0.2556313662804257
1106	20-21	0.3045247233303314
1106	22-23	0.2727342520902454
1106	24-25	0.2979694906704182
1106	26-27	0.2685163053532307
1106	28-29	0.2845067308460969
1106	30-31	0.23966336256589926
1106	32-33	0.19201338341152052
1106	34-35	0.22967732336082491
1106	36-37	0.186475935166726
1106	38-39	0.1556816353438819
1106	40-41	0.19895739147066394
1106	42-43	0.2185427725154625
1106	44-45	0.19177113859277028
1106	46-47	0.16611888042753975
1106	48-49	0.14600224141660334
1106	50-51	0.0292850530572224
1106	52-53	0.057014208452521586
1106	54-55	-0.008076850174347783
1106	56-57	-0.06677695806103401
1106	58-59	0.0301443797537857
1106	60-61	-0.010246234340009153
1106	62-63	-0.046598840778159456
1106	64-65	-0.11149139884825843
1106	66-67	-0.09505374494194285
1106	68-69	-0.06928890467012394
1106	70-71	-0.09549710800434497
1106	72-73	-0.08158048319574362
1106	74-75	-0.07544766715468043
1106	76-77	-0.046229221918924424
1106	78-79	0.009964967867297503
1106	80-81	0.02143493670583041
1106	82-83	0.06626318282120991
1106	84-85	0.045636271441189535
1106	86-87	0.001969881985967703
1106	88-89	-0.06025836660628414
1106	90-91	-0.05191372631703928
1106	92-93	0.0014627351207323613
1106	94-95	-0.026096165462398346
1106	96-97	-0.03151515192840648
1106	98-99	0.030253280961549223
1106	100	0.0036583463305106534
1107	1	1.6387658427658565
1107	2	1.069917862942571
1107	3	1.4113982755005772
1107	4	0.7322562591867623
1107	5	1.4370136016360746
1107	6	0.9519953418362093
1107	7	0.47919189650841787
1107	8	0.35338908052835194
1107	9	0.3772445213088247
1107	10-11	0.22426522626661693
1107	12-13	0.2017462126523526
1107	14-15	0.3053869742042181
1107	16-17	0.2715517424668974
1107	18-19	0.25801717014218184
1107	20-21	0.23626272844506957
1107	22-23	0.26974667507997907
1107	24-25	0.33337053136227013
1107	26-27	0.26911996662688153
1107	28-29	0.2855279104651416
1107	30-31	0.24316317967500822
1107	32-33	0.24208703970712264
1107	34-35	0.19797483344312639
1107	36-37	0.2422954863190938
1107	38-39	0.23776695355461186
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2216	98-99	-0.6395130606378281
2216	100	-0.6842766322806497
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	7355.0
3	2063.0
4	2189.0
5	2764.0
6	2779.0
7	2891.0
8	3433.0
9	4190.0
10	5572.0
11	13003.0
12	9600.0
13	14258.0
14	13593.0
15	14527.0
16	17340.0
17	23432.0
18	33768.0
19	60103.0
20	132293.0
21	185141.0
22	89812.0
23	58231.0
24	53777.0
25	54246.0
26	53650.0
27	56240.0
28	62415.0
29	71323.0
30	81730.0
31	98587.0
32	123012.0
33	161576.0
34	228012.0
35	372952.0
36	635718.0
37	1188709.0
38	1762928.0
39	634557.0
40	2785.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	14.207958994623409	43.33689458127457	25.877384707885614	16.577761716216408
2	17.515209249092052	41.293198086699505	24.23983740607259	16.95175525813585
3	18.740368545141095	23.292195491465808	20.253880063773614	37.713555899619486
4	35.957551974165035	24.206165581114835	21.951867297400195	17.884415147319935
5	17.416112219847037	40.37991002048086	23.05101415428368	19.152963605388425
6	38.53683763279991	21.639891403811088	23.28872208958397	16.534548873805033
7	18.596687292624587	21.67220719198985	41.77431498887952	17.956790526506044
8	37.71083725491495	21.861370473305644	22.872402001465485	17.55539027031392
9	37.311708093646075	23.55524454172301	22.497687110621563	16.63536025400935
10-11	16.71910687930424	43.46772379826747	22.785524734904868	17.027644587523426
12-13	16.67726510964184	23.18758739378294	32.65351891964015	27.481628576935073
14-15	16.93375689253652	33.01892862989575	22.742823418899988	27.304491058667747
16-17	27.241570662222674	22.842968553903496	32.72955013276848	17.185910651105342
18-19	17.159730837400016	22.99772228104989	32.831752556637795	27.0107943249123
20-21	27.29873382904342	33.2805091810879	22.63624010217436	16.784516887694316
22-23	26.959348680543133	23.274964792061628	32.83947459086519	16.926211936530056
24-25	16.903199625774025	33.15793856498975	22.75294083135322	27.185920977883004
26-27	17.244713162473772	27.302576299495655	38.59200357075686	16.860706967273714
28-29	26.21489009978154	23.04941493981194	33.89314709569898	16.842547864707548
30-31	23.991168037874157	24.420006201742105	34.23319843650485	17.355627323878885
32-33	19.876734408119418	25.94511545739892	35.79905260749799	18.379097526983667
34-35	19.29236893805817	25.66722633092174	36.10156979633095	18.938834934689144
36-37	19.061340995963114	26.328502339261906	35.94825358980725	18.661903074967736
38-39	19.424719387525744	26.047790519343454	35.87292992058258	18.65456017254822
40-41	19.271054552027167	26.397710473757684	35.71481294963007	18.616422024585074
42-43	19.56610563756995	26.241325870610865	35.68632117860984	18.506247313209347
44-45	19.162095023464047	26.419102004599203	35.73957630257627	18.679226669360485
46-47	19.326077361372214	26.431417075283974	35.59385535881764	18.648650204526177
48-49	19.151813558248733	26.757541926579798	35.50079613244685	18.589848382724618
50-51	19.224520379508654	26.826199860582157	35.07317780648996	18.876101953419237
52-53	19.3381040614277	27.05009769204515	34.52589706658894	19.08590117993821
54-55	19.974569646387337	27.634927433041273	33.140258320598306	19.250244599973076
56-57	20.445808162832392	28.296693916062715	31.5146934216228	19.7428044994821
58-59	20.552705658079752	29.17194064835612	30.60448958016832	19.670864113395805
60-61	20.602680840489526	29.221979929933706	30.369652019431438	19.805687210145333
62-63	20.28926911558652	29.58452489327219	30.225656690683973	19.900549300457318
64-65	20.328861759765303	29.521864969519168	30.375091679087706	19.774181591627826
66-67	20.23532505187642	29.31562086123335	30.50303505939792	19.946019027492316
68-69	20.153060600795627	29.411746612834065	30.531801870302527	19.90339091606778
70-71	19.984043945082526	29.42191004829246	30.594457684198506	19.999588322426508
72-73	19.929879725543902	29.455988242733056	30.524818504762198	20.08931352696085
74-75	20.1159117242628	29.373819135410166	30.40570522954403	20.104563910783003
76-77	20.162337716106784	29.454668076813185	30.318689065294773	20.064305141785262
78-79	20.237714405777872	29.86030597375376	29.783635865174183	20.118343755294188
80-81	20.09331429223978	29.91405167089066	29.720359963734854	20.272274073134707
82-83	20.241967219837495	30.113097317635408	29.378410328519276	20.26652513400782
84-85	20.34664797966511	30.186549838207917	29.219874465240725	20.246927716886248
86-87	20.363823111877103	30.463322542996853	28.85958259402297	20.31327175110308
88-89	20.463004532841474	30.387466112516893	28.69799118604081	20.45153816860082
90-91	20.504929216252723	30.659324470880527	28.438440631429255	20.39730568143749
92-93	20.47983480993276	30.715836506187806	28.362988484661738	20.441340199217695
94-95	20.476068649330465	30.82084211818033	28.20490698012252	20.498182252366686
96-97	20.337620667534512	30.73965466304575	28.17526696664283	20.7474577027769
98-99	20.495612328537458	30.906031011710816	27.732521983307308	20.86583467644442
100	20.69747752573118	30.935016013281256	27.58957611568551	20.77793034530205
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	22604.0
1	13118.5
2	3034.0
3	2347.0
4	2188.5
5	2074.5
6	2136.5
7	2381.0
8	2435.0
9	2486.0
10	2635.0
11	3702.0
12	29726.5
13	38235.0
14	22641.5
15	25446.0
16	29087.0
17	32605.5
18	36213.5
19	39940.0
20	43711.5
21	47867.5
22	53027.5
23	59678.5
24	66848.0
25	74000.0
26	84078.5
27	94565.5
28	101043.5
29	106680.5
30	114454.5
31	123355.5
32	132927.0
33	144246.5
34	156922.0
35	166635.0
36	174366.5
37	181536.5
38	187939.0
39	194284.0
40	199928.5
41	205045.5
42	208308.5
43	210628.0
44	213323.0
45	214039.0
46	211373.0
47	209154.0
48	205837.0
49	200060.0
50	192796.0
51	182245.0
52	170234.0
53	158978.0
54	147221.5
55	133869.0
56	119686.0
57	106444.5
58	98852.5
59	84164.0
60	63402.5
61	49775.5
62	38244.5
63	28430.0
64	20807.5
65	15373.5
66	11324.5
67	8181.5
68	6016.5
69	4384.5
70	3157.5
71	2281.0
72	1664.0
73	1082.0
74	725.0
75	517.5
76	389.5
77	273.0
78	169.5
79	111.5
80	62.0
81	28.0
82	17.5
83	11.5
84	9.0
85	8.0
86	6.0
87	3.5
88	0.0
89	1.5
90	1.5
91	0.0
92	0.5
93	0.5
94	0.5
95	0.5
96	0.5
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.06031018740633705
2	0.0674704450115873
3	1.577149252257768E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	8.674320887417724E-5
18-19	0.0
20-21	3.154298504515536E-4
22-23	1.4982917896448796E-4
24-25	0.0
26-27	0.0025470960423962952
28-29	8.280033574353283E-4
30-31	0.006308597009031072
32-33	0.0015534920134739015
34-35	0.0022947521620350526
36-37	0.008201176111740394
38-39	0.012002105809681614
40-41	0.0032016129820832693
42-43	0.00801980394773075
44-45	0.001726978431222256
46-47	0.0026417249975317614
48-49	0.0033829851460929123
50-51	0.009770439617736873
52-53	0.0035012713400122447
54-55	0.001569263505996479
56-57	7.333744022998621E-4
58-59	0.0011434332078868817
60-61	0.006553055143131026
62-63	0.001987208057844788
64-65	0.007948832231379151
66-67	0.011473760810175263
68-69	0.012262335436304147
70-71	0.010109526706972292
72-73	0.004810305219386192
74-75	0.001979322311583499
76-77	0.00496013439835068
78-79	0.016654696103842032
80-81	0.00917900864814021
82-83	0.006923685217411602
84-85	0.005015334622179702
86-87	0.007373172754305065
88-89	0.004266188727357263
90-91	0.006277054023985916
92-93	0.011126787974678552
94-95	0.00891089327525639
96-97	0.011308160138688197
98-99	0.002760011191451094
100	0.0030281265643349146
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6340554.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.70376759578333
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.02729574625414	59.59779573289676
2	8.95301467939557	12.123056502708685
3	1.4111601130502776	2.8662256900318592
4	0.40894457747385854	1.107483545313837
5	0.1913385632874425	0.6477170810462044
6	0.12317327255103909	0.5003576771284577
7	0.09719170253993384	0.4606171108701529
8	0.0760430084138943	0.41187185351507966
9	0.06322038994529182	0.38522327293537506
>10	0.6107181405494202	8.181426045671133
>50	0.025369732618410447	1.2057087522085224
>100	0.010891064413946949	1.2174464058027443
>500	0.0010068259382130032	0.4772390300049823
>1k	4.2145571242364156E-4	0.5172763940960138
>5k	7.024261871788205E-5	0.30422148501367136
>10k+	1.404852374357641E-4	9.99633342075666
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	550876	8.688136714867502	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	26912	0.4244424067676105	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	21137	0.3333620374497244	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA	11183	0.1763726008799862	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	10454	0.16487518283102706	No Hit
AACGTGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	10030	0.15818807000145413	No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6768	0.10674146139280573	No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6351	0.10016474901089084	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0014036628345094136	0.0	0.0	0.0	1.577149252257768E-5
2	0.0014036628345094136	0.0	0.0	0.0	1.577149252257768E-5
3	0.0014036628345094136	0.0	0.0	0.0	1.577149252257768E-5
4	0.0014194343270319911	1.577149252257768E-5	0.0	1.577149252257768E-5	1.577149252257768E-5
5	0.0014194343270319911	6.308597009031072E-5	0.0	1.577149252257768E-5	1.577149252257768E-5
6	0.001435205819554569	6.308597009031072E-5	0.0	1.577149252257768E-5	1.577149252257768E-5
7	0.001435205819554569	6.308597009031072E-5	0.0	1.577149252257768E-5	1.577149252257768E-5
8	0.001435205819554569	7.88574626128884E-5	0.0	1.577149252257768E-5	1.577149252257768E-5
9	0.001435205819554569	7.88574626128884E-5	0.0	1.577149252257768E-5	1.577149252257768E-5
10-11	0.001435205819554569	9.462895513546608E-5	0.0	3.9428731306444197E-5	1.577149252257768E-5
12-13	0.001435205819554569	9.462895513546608E-5	0.0	6.308597009031072E-5	1.577149252257768E-5
14-15	0.001435205819554569	9.462895513546608E-5	0.0	7.88574626128884E-5	1.577149252257768E-5
16-17	0.001435205819554569	1.0251470139675491E-4	0.0	7.88574626128884E-5	1.577149252257768E-5
18-19	0.0014430915658158578	1.1040044765804376E-4	0.0	9.462895513546608E-5	1.577149252257768E-5
20-21	0.0014509773120771467	1.182861939193326E-4	0.0	9.462895513546608E-5	7.097171635159956E-5
22-23	0.0014509773120771467	1.340576864419103E-4	0.0	9.462895513546608E-5	8.674320887417725E-5
24-25	0.0014509773120771467	1.4194343270319912E-4	0.0	1.2617194018062143E-4	1.0251470139675491E-4
26-27	0.0014746345508610131	1.4194343270319912E-4	0.0	1.4194343270319912E-4	1.1040044765804376E-4
28-29	0.001482520297122302	1.4194343270319912E-4	0.0	1.8137216400964333E-4	1.2617194018062143E-4
30-31	0.0015929207447803457	1.577149252257768E-4	0.0	1.8925791027093217E-4	1.577149252257768E-4
32-33	0.0017033211924383893	1.97143656532221E-4	0.0	1.97143656532221E-4	1.577149252257768E-4
34-35	0.0018610361176641661	2.365723878386652E-4	0.0	2.0502940279350985E-4	1.6560067148706564E-4
36-37	0.002121265744286698	2.365723878386652E-4	0.0	2.2080089531608752E-4	1.8925791027093217E-4
38-39	0.0027678969377123827	2.4445813409995404E-4	0.0	2.2868664157737638E-4	2.365723878386652E-4
40-41	0.0034854998474896674	2.5234388036124287E-4	0.0	2.365723878386652E-4	2.365723878386652E-4
42-43	0.00441601790632175	2.602296266225317E-4	0.0	2.365723878386652E-4	2.365723878386652E-4
44-45	0.005259792756279656	2.681153728838206E-4	0.0	2.365723878386652E-4	2.5234388036124287E-4
46-47	0.006616141113221337	2.917726116676871E-4	0.0	2.365723878386652E-4	2.681153728838206E-4
48-49	0.008145975887911373	3.154298504515536E-4	0.0	2.5234388036124287E-4	2.917726116676871E-4
50-51	0.01013318394575616	3.2331559671284246E-4	0.0	2.5234388036124287E-4	3.154298504515536E-4
52-53	0.01244370760031379	3.706300742805755E-4	0.0	2.602296266225317E-4	3.2331559671284246E-4
54-55	0.01550337714969386	3.7851582054186433E-4	0.0	2.8388686540639825E-4	3.312013429741313E-4
56-57	0.019359507071464103	3.7851582054186433E-4	0.0	2.917726116676871E-4	3.7851582054186433E-4
58-59	0.023972668634318073	3.94287313064442E-4	0.0	3.154298504515536E-4	3.94287313064442E-4
60-61	0.030052579001771766	3.94287313064442E-4	0.0	3.390870892354201E-4	4.2583029810959737E-4
62-63	0.037102436159364	4.1005880558701966E-4	0.0	3.706300742805755E-4	4.2583029810959737E-4
64-65	0.04627355906124292	4.337160443708862E-4	0.0	3.8640156680315316E-4	4.337160443708862E-4
66-67	0.05795234927421169	4.573732831547527E-4	0.0	4.100588055870197E-4	4.4948753689346387E-4
68-69	0.07455973090048598	4.573732831547527E-4	0.0	4.337160443708862E-4	4.6525902941604153E-4
70-71	0.09438449700136613	4.8103052193861924E-4	0.0	4.4160179063217503E-4	4.96802014461197E-4
72-73	0.11980025720150006	4.96802014461197E-4	0.0	4.4948753689346387E-4	5.046877607224857E-4
74-75	0.15186370149990047	5.520022382902188E-4	0.0	4.6525902941604153E-4	5.125735069837745E-4
76-77	0.1930588399688734	5.677737308127965E-4	0.0	5.046877607224857E-4	5.835452233353742E-4
78-79	0.2457592822330667	5.756594770740854E-4	0.0	5.204592532450634E-4	6.229739546418184E-4
80-81	0.31150274881343176	5.993167158579518E-4	0.0	5.441164920289301E-4	6.466311934256849E-4
82-83	0.39325428030421317	6.466311934256849E-4	0.0	5.677737308127965E-4	6.702884322095514E-4
84-85	0.4931982284197879	6.624026859482626E-4	0.0	5.677737308127965E-4	7.018314172547067E-4
86-87	0.6174697037514387	6.860599247321291E-4	0.0	5.677737308127965E-4	7.176029097772845E-4
88	0.7261195157394764	6.939456709934179E-4	0.0	5.677737308127965E-4	7.254886560385733E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGAGT	116700	0.0	92.97413	1
ACGAGTG	124980	0.0	90.8064	2
CGAGTGG	128990	0.0	89.29671	3
GAGTGGA	133800	0.0	87.18236	4
GTGGAAT	137230	0.0	86.86271	6
TGGAATC	138775	0.0	86.38328	7
GAATCAC	139100	0.0	86.31658	9
TACGAGT	5150	0.0	86.16575	1
GGAATCA	139455	0.0	86.08337	8
AGTGGAA	135640	0.0	85.9512	5
ATCGAGT	1935	0.0	72.71439	1
TCGAGTG	2380	0.0	67.37877	2
AACGTGT	2895	0.0	66.31958	1
CGTGTGG	3750	0.0	65.41384	3
ACGTGTG	3755	0.0	59.61323	2
CGATTGG	1105	0.0	50.182255	3
GACGAGT	1385	0.0	48.586594	1
ACGATTG	1055	0.0	48.1412	2
ATCACTG	140900	0.0	43.80427	10-11
TGAGTAC	139245	0.0	43.651978	18-19
>>END_MODULE