FastQCFastQC Report
Thu 26 May 2016
SRR2132662_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132662_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2872725
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1988876.923287122853737No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1281954.462487707664326No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG213350.7426746381919606No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149460.520272563506775No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT110100.3832597968827507No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA72760.2532786813913619No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65180.22689258456691816No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT51670.17986406634815374No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC35550.12375009790355847No Hit
CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT30760.10707603407914087No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT27600.085.913671
AACGAGT631600.066.786281
ACGAGTG672000.066.315762
GTGGAAT684600.066.1009456
TGGAATC691450.065.4528967
CGAGTGG685400.065.3735353
GGAATCA694850.065.200268
GAATCAC695400.065.168969
AGTGGAA697150.064.4863055
GAGTGGA704050.063.948874
ATCTGCG10700.050.0760294
ACGTGTG9300.044.977272
AACGTGT9250.042.8095741
ATCGAGT4650.042.5794141
AGTACTT288800.040.9211720-21
GAGTACT285050.040.67632320-21
CGTGTGG10350.040.4129563
GTACTTT289100.039.42231822-23
TACTTTT306950.038.18627522-23
CATGGGG128450.037.19089524-25