##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132662_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2872725
Sequences flagged as poor quality	0
Sequence length	100
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.585006918518133	33.0	30.0	34.0	16.0	34.0
2	31.164142756442054	33.0	31.0	34.0	25.0	34.0
3	31.94586290020799	34.0	31.0	34.0	28.0	34.0
4	35.992277715409585	37.0	35.0	37.0	35.0	37.0
5	35.75758521960856	37.0	35.0	37.0	35.0	37.0
6	36.1837711580468	37.0	37.0	37.0	35.0	37.0
7	36.23284686142948	37.0	36.0	37.0	35.0	37.0
8	36.3677525694245	37.0	37.0	37.0	35.0	37.0
9	38.29092655927734	39.0	39.0	39.0	37.0	39.0
10-11	38.32575063746095	39.0	39.0	39.0	37.0	39.0
12-13	38.28517940283181	39.0	39.0	39.0	37.0	39.0
14-15	39.811603790825785	41.0	40.0	41.0	38.0	41.0
16-17	39.65181474035977	41.0	39.5	41.0	37.5	41.0
18-19	39.359457831849554	41.0	39.0	41.0	36.0	41.0
20-21	39.22015107606889	41.0	39.0	41.0	35.0	41.0
22-23	38.610408758234776	40.5	39.0	41.0	35.0	41.0
24-25	38.54716062275365	41.0	39.0	41.0	35.0	41.0
26-27	38.50218520742501	41.0	39.0	41.0	35.0	41.0
28-29	38.407901208782604	41.0	39.0	41.0	35.0	41.0
30-31	38.276125803896996	41.0	38.0	41.0	35.0	41.0
32-33	38.15693026655876	40.5	38.0	41.0	35.0	41.0
34-35	38.06154992211228	40.0	38.0	41.0	35.0	41.0
36-37	37.911514850882	40.0	38.0	41.0	35.0	41.0
38-39	37.51562053450992	40.0	36.0	41.0	34.0	41.0
40-41	37.33541254383904	40.0	35.0	41.0	33.0	41.0
42-43	37.2163513736957	40.0	35.0	41.0	33.0	41.0
44-45	37.05600866772838	40.0	35.0	41.0	33.0	41.0
46-47	36.94819187356952	40.0	35.0	41.0	33.0	41.0
48-49	36.79282232027082	40.0	35.0	41.0	33.0	41.0
50-51	36.554700850238014	39.5	35.0	41.0	33.0	41.0
52-53	36.07653482320793	39.0	35.0	41.0	31.0	41.0
54-55	35.255457448937854	39.0	35.0	41.0	27.0	41.0
56-57	34.66667275844364	39.0	35.0	41.0	22.5	41.0
58-59	34.336036515851674	38.5	35.0	41.0	20.0	41.0
60-61	34.08858540236187	38.0	35.0	40.5	19.0	41.0
62-63	33.809764074180436	37.0	35.0	40.0	18.0	41.0
64-65	33.510227223281035	37.0	35.0	40.0	15.5	41.0
66-67	33.17401230538948	36.0	34.0	39.5	12.0	41.0
68-69	32.87172318965442	35.5	34.0	39.0	9.0	41.0
70-71	32.556051658268714	35.0	34.0	39.0	7.0	41.0
72-73	32.21779721344716	35.0	34.0	37.5	2.0	40.0
74-75	32.03762177027039	35.0	34.0	37.0	2.0	39.5
76-77	31.743371189375942	35.0	34.0	37.0	2.0	39.0
78-79	31.425012314092022	35.0	34.0	36.0	2.0	39.0
80-81	31.145184798405694	35.0	33.5	36.0	2.0	38.5
82-83	30.865495130929695	35.0	33.0	35.5	2.0	37.0
84-85	30.58197478004334	35.0	33.0	35.0	2.0	37.0
86-87	30.398534666562234	35.0	33.0	35.0	2.0	36.5
88-89	30.210608046367128	35.0	33.0	35.0	2.0	36.0
90-91	30.042250650514752	35.0	33.0	35.0	2.0	36.0
92-93	29.900438259840396	35.0	33.0	35.0	2.0	36.0
94-95	29.729397871359076	35.0	33.0	35.0	2.0	35.0
96-97	29.544499212420263	35.0	33.0	35.0	2.0	35.0
98-99	29.41250467761446	35.0	33.0	35.0	2.0	35.0
100	29.32166392536703	35.0	33.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	3.390730145896491
1101	2	2.108419497512749
1101	3	1.3955394238872358
1101	4	0.6097951753269513
1101	5	0.8501106308415771
1101	6	0.3901487240613193
1101	7	0.23502815322945025
1101	8	0.16989814846252216
1101	9	0.18554175916732163
1101	10-11	0.11889451855225275
1101	12-13	0.17966518843682167
1101	14-15	0.2368311617939156
1101	16-17	0.0933804441015127
1101	18-19	0.08848007144229797
1101	20-21	0.1308872205220979
1101	22-23	-0.09005795846211129
1101	24-25	-0.05899083252189996
1101	26-27	0.009755798717634434
1101	28-29	0.004268179769525204
1101	30-31	0.05385535041350664
1101	32-33	0.030250549924097925
1101	34-35	-0.035949061943732374
1101	36-37	-0.04057553322687113
1101	38-39	0.009128753678851353
1101	40-41	0.042895168834981234
1101	42-43	0.04439506171031127
1101	44-45	0.09179478611483205
1101	46-47	0.06234538045379168
1101	48-49	0.06851421601159302
1101	50-51	0.13846861600911353
1101	52-53	0.2234412774649428
1101	54-55	0.23717350555005368
1101	56-57	0.07102858688543989
1101	58-59	0.013353322451436611
1101	60-61	0.019006121630397388
1101	62-63	0.07650623436298076
1101	64-65	0.10018671359154752
1101	66-67	0.05429948172958632
1101	68-69	0.04367014999952801
1101	70-71	0.07572407686559757
1101	72-73	0.12448249827617985
1101	74-75	0.0766438915262313
1101	76-77	0.04819413630695735
1101	78-79	-0.049399549229860895
1101	80-81	0.06512971201639317
1101	82-83	0.01335416744136353
1101	84-85	0.04574778876656538
1101	86-87	-0.030177259997174133
1101	88-89	-7.16266197287041E-4
1101	90-91	0.018040129019588846
1101	92-93	0.03009755410888104
1101	94-95	0.06767971971213527
1101	96-97	0.03747758494345632
1101	98-99	0.07120328461448722
1101	100	0.04124419305595595
1102	1	3.4094048135904202
1102	2	2.0579471573938193
1102	3	1.362089256039269
1102	4	0.5959075077981097
1102	5	0.8719195763410568
1102	6	0.4013235917431146
1102	7	0.24889942459056158
1102	8	0.2588002690146425
1102	9	0.20667505774153483
1102	10-11	0.20165488430593825
1102	12-13	0.15471629171735657
1102	14-15	0.19811470372923878
1102	16-17	0.2639504293563135
1102	18-19	0.17811922526388457
1102	20-21	0.16007112254904143
1102	22-23	0.1253836856480035
1102	24-25	0.15299782972668652
1102	26-27	0.14710751693956325
1102	28-29	0.12205819195034451
1102	30-31	0.14399322573114404
1102	32-33	0.16952101690852572
1102	34-35	0.12735393186033406
1102	36-37	0.1460123164753
1102	38-39	0.14689477299001652
1102	40-41	0.20925891299349075
1102	42-43	0.126661726939723
1102	44-45	0.10259308091339392
1102	46-47	0.04733687316884527
1102	48-49	0.16443117310979716
1102	50-51	0.22830977175171796
1102	52-53	0.21713550425514683
1102	54-55	0.10575615046182207
1102	56-57	0.1834718314657735
1102	58-59	0.16063414001315124
1102	60-61	0.1811516254210872
1102	62-63	0.15844823845124267
1102	64-65	0.12609909791771656
1102	66-67	0.12728458277593546
1102	68-69	0.12214700275229973
1102	70-71	0.08598169526833743
1102	72-73	0.0699736939879898
1102	74-75	0.12201608737023406
1102	76-77	0.15497883079642705
1102	78-79	0.1299097636232034
1102	80-81	0.16734268405156527
1102	82-83	0.18282067358822474
1102	84-85	0.17040078855608343
1102	86-87	0.21663127417975758
1102	88-89	0.2569700473144607
1102	90-91	0.28129345208025924
1102	92-93	0.2718710180744708
1102	94-95	0.24967715305944793
1102	96-97	0.20445102258440784
1102	98-99	0.19891079400257183
1102	100	0.20908251436070557
1103	1	3.385996603825994
1103	2	2.0688949223679813
1103	3	1.2961969694736908
1103	4	0.61127312986698
1103	5	0.8781736811269667
1103	6	0.43212571265349453
1103	7	0.27097176127063705
1103	8	0.2185969605331195
1103	9	0.18997741458682071
1103	10-11	0.11223747982105436
1103	12-13	0.1670856367427902
1103	14-15	0.14565472117879352
1103	16-17	0.21103013298355222
1103	18-19	0.17675407699083223
1103	20-21	0.10667471916005411
1103	22-23	0.136788297645829
1103	24-25	0.1605610362072909
1103	26-27	0.16162608453539917
1103	28-29	0.14452015828002374
1103	30-31	0.20326546765205933
1103	32-33	0.17623639851638728
1103	34-35	0.24653091385801673
1103	36-37	0.19148149952419402
1103	38-39	0.14053358264543192
1103	40-41	0.16722359401912001
1103	42-43	0.13930225266047813
1103	44-45	0.2422459388073861
1103	46-47	0.10784560661247156
1103	48-49	0.20595822088035476
1103	50-51	0.25206755038122
1103	52-53	0.19011431248532773
1103	54-55	0.17320791862543672
1103	56-57	0.12566007478110208
1103	58-59	0.04457419521228445
1103	60-61	-0.00772226207359239
1103	62-63	0.053826225435336994
1103	64-65	0.08084813058459872
1103	66-67	0.07556206534082577
1103	68-69	0.09163955972816495
1103	70-71	0.14056316599988605
1103	72-73	0.1304747234345882
1103	74-75	0.1422144267736769
1103	76-77	0.1571242013683154
1103	78-79	0.11992384267776401
1103	80-81	0.11515311179674015
1103	82-83	0.17267007970635362
1103	84-85	0.18049398766011038
1103	86-87	0.17894698012589672
1103	88-89	0.15572585179472043
1103	90-91	0.17135317731720434
1103	92-93	0.06661862550189213
1103	94-95	0.09835216187089202
1103	96-97	0.12770495980838703
1103	98-99	0.17045858478512343
1103	100	0.14616782264577566
1104	1	2.61133917415836
1104	2	1.7823635448883195
1104	3	1.2272907870199887
1104	4	0.5374692955187044
1104	5	0.8248227902740268
1104	6	0.33816071005058035
1104	7	0.24003532952386308
1104	8	0.18596519409685897
1104	9	0.16782143058541976
1104	10-11	0.17131035229738956
1104	12-13	0.174068234917371
1104	14-15	0.12596115838679367
1104	16-17	0.20004210639143594
1104	18-19	0.18474019265051567
1104	20-21	0.09143088015305523
1104	22-23	0.024592736570454576
1104	24-25	0.052434559098571754
1104	26-27	0.1065206893299333
1104	28-29	0.15607750711364332
1104	30-31	0.13618088519668703
1104	32-33	0.1025237680115012
1104	34-35	0.13271281053908268
1104	36-37	0.13173753298593738
1104	38-39	0.1888565107412461
1104	40-41	0.17856509742614435
1104	42-43	0.16051716381186054
1104	44-45	0.18966562961259825
1104	46-47	0.18940262079348713
1104	48-49	0.14517509745530077
1104	50-51	0.12115238668820183
1104	52-53	0.09545661201327249
1104	54-55	0.0525856618273437
1104	56-57	0.05979433234274012
1104	58-59	0.042660648309684746
1104	60-61	-0.013516293082012965
1104	62-63	-0.029914534602561105
1104	64-65	-0.0550190110098967
1104	66-67	-0.07103581113427282
1104	68-69	-0.03278961814817194
1104	70-71	-0.07281659194280365
1104	72-73	-0.06174744912792107
1104	74-75	-0.09204470904982287
1104	76-77	-0.05300461016249258
1104	78-79	-0.03753216233850054
1104	80-81	-0.08730951306638701
1104	82-83	-0.055243398724570625
1104	84-85	-0.12489077091720802
1104	86-87	-0.13537795676621656
1104	88-89	-0.06377975512254608
1104	90-91	0.03691441890919478
1104	92-93	-0.046469296654390035
1104	94-95	0.0060428640436143155
1104	96-97	0.02984543750516977
1104	98-99	0.05379552150506939
1104	100	0.05355568622684359
1105	1	3.315866000502723
1105	2	2.0142337347961146
1105	3	1.2775665251859891
1105	4	0.578802990974502
1105	5	0.8345298202971421
1105	6	0.3703874291312985
1105	7	0.1955914641101657
1105	8	0.14536853750929168
1105	9	0.17299194691157993
1105	10-11	0.10956339935358983
1105	12-13	0.11845145166550708
1105	14-15	0.15090638862521644
1105	16-17	0.10356372394578273
1105	18-19	0.11942259614756523
1105	20-21	0.10787459355316287
1105	22-23	0.038029532487236395
1105	24-25	0.031957539153111725
1105	26-27	0.001806570564937715
1105	28-29	-0.003041962270273757
1105	30-31	0.059964449413726584
1105	32-33	0.10012095859275405
1105	34-35	0.0703862111771727
1105	36-37	0.02845316366988726
1105	38-39	0.10552519560637563
1105	40-41	0.017411905675913886
1105	42-43	-0.027512052421847955
1105	44-45	-0.06749914609221008
1105	46-47	-0.059175900767314715
1105	48-49	-0.1380293306595206
1105	50-51	-0.07132736258235894
1105	52-53	-0.07257619481172384
1105	54-55	-0.1039953373603737
1105	56-57	-0.19987191024997486
1105	58-59	-0.09881462196583612
1105	60-61	-0.1700702628261226
1105	62-63	-0.15369424982682744
1105	64-65	-0.16448323013521815
1105	66-67	-0.21426602209475476
1105	68-69	-0.18508085542960373
1105	70-71	-0.1736518504032034
1105	72-73	-0.0976205212393424
1105	74-75	-0.10339539953858079
1105	76-77	-0.036334254877754546
1105	78-79	-0.09023641492411016
1105	80-81	-0.05537373891663222
1105	82-83	-0.041239795375386024
1105	84-85	-0.020045991821824316
1105	86-87	-0.030095841273297452
1105	88-89	-0.062708389066362
1105	90-91	-0.02737884276944058
1105	92-93	0.02073519827721171
1105	94-95	3.92575093457026E-4
1105	96-97	0.07124578756890187
1105	98-99	0.12109448434206982
1105	100	0.07749155595270807
1106	1	1.7656167709751323
1106	2	1.4096120939710133
1106	3	0.9869589592380095
1106	4	0.3757685641500643
1106	5	0.647278115331801
1106	6	0.212653607481343
1106	7	0.09420803762769481
1106	8	0.05398963009853475
1106	9	0.09307882281950697
1106	10-11	0.09474467108906026
1106	12-13	0.025770850566139814
1106	14-15	0.06619366170048835
1106	16-17	0.08429214993300604
1106	18-19	0.04853434412157043
1106	20-21	0.016846448196531583
1106	22-23	-0.11796115881137581
1106	24-25	-0.09242082672290763
1106	26-27	-0.04989292917507271
1106	28-29	-0.02585487186349411
1106	30-31	-0.007285222178758488
1106	32-33	-0.07640986124601312
1106	34-35	-0.11508898868913775
1106	36-37	0.013396205147557794
1106	38-39	-0.002062609559231987
1106	40-41	-0.01622295563277021
1106	42-43	-0.1150164526532862
1106	44-45	0.06379425809436157
1106	46-47	-0.02479794742934871
1106	48-49	0.006069034956837527
1106	50-51	0.003469530482540506
1106	52-53	-0.03004773544451922
1106	54-55	-0.04132360792334566
1106	56-57	-0.07141774165760495
1106	58-59	-0.11659192345178582
1106	60-61	-0.16693083773665052
1106	62-63	-0.16901438459484552
1106	64-65	-0.17044526888572875
1106	66-67	-0.19697638456911903
1106	68-69	-0.09497869571957551
1106	70-71	-0.221836949947388
1106	72-73	-0.20086280337474705
1106	74-75	-0.26746757003532196
1106	76-77	-0.2943672817951324
1106	78-79	-0.19522091124131435
1106	80-81	-0.1872904001560869
1106	82-83	-0.22226403418508056
1106	84-85	-0.16291773729966152
1106	86-87	-0.10970210092056476
1106	88-89	-0.09264365304988331
1106	90-91	-0.06465503302026931
1106	92-93	-0.12969662122293713
1106	94-95	-0.09123812188435565
1106	96-97	-0.06364905292022272
1106	98-99	-0.0626915082946482
1106	100	-0.006475382786646833
1107	1	2.820839010363173
1107	2	1.8330111338326915
1107	3	1.2074396957563067
1107	4	0.49909575588527133
1107	5	0.8117215989893865
1107	6	0.35270592263186273
1107	7	0.17140298717686164
1107	8	0.16537162437231956
1107	9	0.1017345781493404
1107	10-11	0.09786939110994552
1107	12-13	0.08419217344923169
1107	14-15	0.12116434080763128
1107	16-17	0.19843273572635667
1107	18-19	0.14282419360683463
1107	20-21	0.14098814319189756
1107	22-23	-0.04532301928293947
1107	24-25	-0.06981007030019981
1107	26-27	-0.005699137400156928
1107	28-29	-0.002271395279493049
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2216	50-51	-0.08748921539754662
2216	52-53	-0.05538732896086884
2216	54-55	-0.006824921798212813
2216	56-57	0.10797866008993395
2216	58-59	0.13604701486430315
2216	60-61	0.14272852251514223
2216	62-63	0.15135103887486423
2216	64-65	0.17616028285631558
2216	66-67	0.19021557817168144
2216	68-69	0.0620105797167696
2216	70-71	0.05110288602779889
2216	72-73	-0.022212896897670475
2216	74-75	0.0776122105425685
2216	76-77	0.07149990407473439
2216	78-79	0.08116974781213315
2216	80-81	0.0649169184419307
2216	82-83	0.004395057932985225
2216	84-85	0.0018020237040765608
2216	86-87	0.03478568515662417
2216	88-89	-0.03689679267786872
2216	90-91	-0.1425314640073374
2216	92-93	-0.1862211871593935
2216	94-95	-0.15972663384624397
2216	96-97	-0.2701681325188403
2216	98-99	-0.27349959874875296
2216	100	-0.28968352816301746
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	270.0
3	104.0
4	212.0
5	626.0
6	2275.0
7	4897.0
8	18808.0
9	26325.0
10	3238.0
11	5277.0
12	4307.0
13	5654.0
14	5700.0
15	6358.0
16	7424.0
17	9076.0
18	12071.0
19	20495.0
20	45831.0
21	60977.0
22	27342.0
23	15681.0
24	15434.0
25	16563.0
26	16906.0
27	18337.0
28	21423.0
29	26225.0
30	30035.0
31	36490.0
32	47373.0
33	66846.0
34	103918.0
35	275400.0
36	333305.0
37	611674.0
38	742204.0
39	225863.0
40	1781.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	13.631674402447985	42.17442077646414	25.828281364825735	18.365623456262142
2	16.59009467820619	40.283735246345046	25.353938032734614	17.77223204271415
3	17.735005990850464	26.04923427810774	20.772342808925636	35.443416922116164
4	31.7973914342099	26.96450931608261	22.92717466640999	18.310924583297506
5	16.721684115256423	39.36913557684777	23.637869966669278	20.27131034122654
6	34.94699283781079	23.87391762177027	24.567231461417297	16.611858079001646
7	18.65072361607881	24.163712154835565	39.28343297739951	17.902131251686118
8	34.014359188575305	24.231766006143992	24.082430444960796	17.671444360319903
9	33.67081777754571	25.97488447380101	23.936819570268646	16.417478178384634
10-11	16.544256759696804	42.45744023531664	23.888276810345577	17.110026194640977
12-13	16.408236082465255	25.543290777919918	32.09485766998233	25.953615469632492
14-15	16.754202722784424	33.63593262276427	23.80800293519628	25.80186171925502
16-17	25.4283346697534	25.12228374144475	32.139106969707676	17.310274619094173
18-19	17.045636309933762	25.366590275743555	32.307808252343115	25.27996516197956
20-21	25.524058532618703	33.840331977708956	23.886257046015228	16.749352443657113
22-23	25.219326644053446	25.687377813424234	31.844084359561325	17.249211182960995
24-25	16.857809222950333	33.94949046636904	23.284599117562593	25.908101193118032
26-27	17.317564333516085	29.365776396974997	36.04887345638723	17.267785813121687
28-29	25.499847705575718	25.426920432690213	31.967034784046504	17.10619707768756
30-31	22.738688875544995	27.36511500404668	32.20511883316363	17.69107728724469
32-33	19.267489926811653	27.91205214561088	33.39324160857722	19.42721631900025
34-35	19.28506021332482	27.96095189078142	33.45119151699565	19.302796378898105
36-37	19.378172184628216	27.86468037571998	33.73771136076571	19.01943607888609
38-39	19.979824020624335	27.730536497949853	33.44979658672277	18.83984289470304
40-41	18.70474897527609	28.896239633100972	33.45361982090176	18.945391570721178
42-43	18.803843815535703	27.974181946473347	33.892983249141885	19.328990988849064
44-45	18.7501816677368	28.424130070444438	33.22428337625769	19.601404885561074
46-47	19.387564532183983	27.93224270802203	33.639915417389496	19.04027734240449
48-49	18.790018392338677	28.10893096250222	33.633803611178266	19.467247033980833
50-51	19.341847712385764	28.69086287542525	32.88759843061027	19.07969098157871
52-53	19.447610723006292	28.414859712248376	32.774211761986585	19.363317802758743
54-55	19.45137022554056	29.211804629096356	31.13719207977247	20.199633065590614
56-57	19.844471414629936	29.940549364461095	30.130613169855252	20.084366051053713
58-59	20.777290838941717	29.964509103516768	29.231298615922917	20.026901441618598
60-61	20.318045802717194	30.605398883546584	28.79448651915223	20.282068794583992
62-63	19.712770375383045	30.53041386922981	28.752203052230307	21.004612703156837
64-65	20.099386920791222	30.438676066379088	29.29997855684802	20.161958455981665
66-67	20.058564644067665	29.851886172109182	29.784041134491563	20.305508049331593
68-69	20.41251453760074	29.94643061652655	29.538333490559303	20.102721355313406
70-71	19.22882652190955	30.929945409747155	29.530204262648667	20.311023805694624
72-73	19.139718507443725	30.16018776613474	29.906108230084875	20.793985496336663
74-75	19.438957491035946	30.61009874427525	29.100520812392837	20.850422952295965
76-77	20.089671971504085	30.183181805919013	29.21908627533229	20.508059947244615
78-79	19.9129948041948	30.49251192779481	28.786400399345286	20.80809286866511
80-81	20.425093445540504	31.070270176186725	28.162184036586268	20.342452341686503
82-83	20.105467860028995	31.057861990546602	28.425708217306678	20.410961932117726
84-85	20.005900398146334	31.36373169723473	27.700124012792898	20.930243891826034
86-87	20.049799262497466	31.445405752589394	27.952651536579655	20.55214344833348
88-89	21.146668741398436	30.98596231611776	27.633547368371588	20.23382157411222
90-91	20.409574563447176	31.449383404978885	27.58443989841225	20.55660213316169
92-93	19.888947296963284	31.58878472137608	27.46559629035234	21.0566716913083
94-95	20.164527969006276	31.813405468217347	27.266571897089776	20.755494665686598
96-97	20.29844781653817	31.258591766594275	27.52928206336768	20.913678353499872
98-99	20.90486272205498	31.2498607587534	26.908771223847012	20.936505295344606
100	19.992968140804276	32.28138576361814	26.760714882477444	20.964931213100144
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	135273.0
1	76060.0
2	11621.0
3	5265.5
4	4006.0
5	3714.0
6	3737.5
7	4075.5
8	3923.5
9	3652.5
10	3644.5
11	4538.0
12	31697.5
13	34681.0
14	9734.0
15	8199.5
16	8792.0
17	9703.5
18	10714.5
19	11845.0
20	13156.0
21	14475.5
22	15831.5
23	17683.0
24	20086.0
25	22551.5
26	25186.0
27	28426.0
28	31327.0
29	33945.5
30	37303.5
31	40324.0
32	43992.0
33	49607.5
34	54649.5
35	57929.0
36	61381.0
37	64489.5
38	65952.5
39	67935.5
40	69935.0
41	70976.5
42	71406.0
43	73463.5
44	76692.0
45	78222.5
46	79569.5
47	83428.0
48	86717.0
49	92629.5
50	117989.0
51	118116.0
52	89360.0
53	80209.0
54	80057.5
55	79926.0
56	75215.0
57	68002.0
58	61229.0
59	52050.5
60	43108.0
61	35570.0
62	28457.5
63	21581.0
64	15654.0
65	11013.5
66	7810.5
67	5754.0
68	4302.5
69	3029.0
70	2072.0
71	1632.0
72	1239.5
73	847.0
74	654.0
75	471.0
76	320.0
77	227.5
78	136.5
79	77.5
80	42.5
81	19.5
82	15.0
83	9.5
84	4.0
85	2.5
86	2.0
87	2.5
88	2.0
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2899685838359049
2	0.0016360772437319967
3	3.8291169534153115E-4
4	0.003898737261659226
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	1.3924061648782948E-4
16-17	1.7405077060978685E-5
18-19	5.221523118293606E-5
20-21	6.962030824391474E-5
22-23	0.002401900634415059
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0022104447867442936
36-37	1.7405077060978685E-5
38-39	6.962030824391474E-5
40-41	0.0
42-43	2.2626600179272293E-4
44-45	0.001235760471329487
46-47	0.0010617097007196998
48-49	0.0021408244785003786
50-51	5.569624659513179E-4
52-53	1.566456935488082E-4
54-55	1.566456935488082E-4
56-57	1.9145584767076557E-4
58-59	5.743675430122967E-4
60-61	0.0019841787849515704
62-63	2.0886092473174425E-4
64-65	4.5253200358544586E-4
66-67	6.962030824391474E-5
68-69	6.962030824391474E-5
70-71	0.0017753178602198261
72-73	8.702538530489345E-5
74-75	5.221523118293606E-5
76-77	0.0011313300089636148
78-79	8.006335448050196E-4
80-81	0.0021756346326223357
82-83	5.221523118293606E-5
84-85	0.0013053807795734016
86-87	0.006700954668476795
88-89	0.0022452549408662507
90-91	0.0046819657294032676
92-93	0.007484183136220836
94-95	0.004995257116500884
96-97	0.0021582295555613576
98-99	4.5253200358544586E-4
100	0.0029588631003663766
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2872725.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.259363965233355
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.55511708568984	46.4449837931628
2	11.485994060064758	12.923894406553831
3	3.0056424177627923	5.072865921907827
4	1.0098333371857675	2.2725032504384126
5	0.49962221724591943	1.4054214082577534
6	0.28493072583920265	0.9618012845919491
7	0.1891983943327171	0.7450926929881452
8	0.1356986272856017	0.6107454769634569
9	0.09940279914472502	0.5033104430621463
>10	0.6555092827718954	7.664010463235568
>50	0.04995290201605697	1.8706276957387553
>100	0.023089206342835605	2.35857549173343
>500	0.003159341857888916	1.3402834830395072
>1k	0.0023540194235231794	2.1176109606291833
>5k	1.858436386991984E-4	0.6608189698077707
>10k+	3.0973939783199734E-4	13.04745425788959
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	198887	6.923287122853737	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	128195	4.462487707664326	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	21335	0.7426746381919606	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14946	0.520272563506775	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	11010	0.3832597968827507	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7276	0.2532786813913619	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6518	0.22689258456691816	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5167	0.17986406634815374	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC	3555	0.12375009790355847	No Hit
CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3076	0.10707603407914087	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.034984204892567164	0.0	0.0	0.0	0.0
2	0.042363957566422125	0.0	0.0	0.0	0.0
3	0.04929117823669164	0.0	0.0	0.0	0.0
4	0.05134497732988713	0.0	0.0	0.0	0.0
5	0.051484217946374956	0.0	0.0	0.0	0.0
6	0.05151902810049692	0.0	0.0	0.0	0.0
7	0.05155383825461887	0.0	0.0	0.0	0.0
8	0.051797509333472574	0.0	0.0	0.0	0.0
9	0.05221523118293606	0.0	0.0	0.0	0.0
10-11	0.05238928195354585	0.0	0.0	0.0	0.0
12-13	0.05252852257003368	0.0	0.0	0.0	0.0
14-15	0.05261554795533857	0.0	0.0	0.0	0.0
16-17	0.052789598725948364	0.0	0.0	0.0	0.0
18-19	0.052876624111253254	0.0	0.0	0.0	0.0
20-21	0.05294624441949716	0.0	0.0	0.0	0.0
22-23	0.053102890113045975	0.0	0.0	0.0	0.0
24-25	0.05325953580659479	0.0	0.0	0.0	0.0
26-27	0.05329434596071674	0.0	0.0	0.0	0.0
28-29	0.05345099165426555	0.0	0.0	0.0	0.0
30-31	0.05353801703957044	0.0	0.0	0.0	0.0
32-33	0.05372947288724121	0.0	0.0	0.0	0.0
34-35	0.05395573888903393	0.0	0.0	0.0	0.0
36-37	0.054321245507314486	0.0	0.0	0.0	0.0
38-39	0.05477377751089993	0.0	0.0	0.0	0.0
40-41	0.05573105674925376	0.0	0.0	0.0	0.0
42-43	0.056792766449973456	0.0	0.0	0.0	0.0
44-45	0.057976311690120005	0.0	0.0	0.0	0.0
46-47	0.05940352800912026	0.0	0.0	0.0	0.0
48-49	0.06090036463636443	0.0	0.0	0.0	0.0
50-51	0.06302378403780383	0.0	0.0	0.0	0.0
52-53	0.06544308974927987	0.0	0.0	0.0	0.0
54-55	0.06819309192491449	0.0	0.0	0.0	0.0
56-57	0.07146524641237849	0.0	0.0	0.0	0.0
58-59	0.07621683245002567	0.0	0.0	0.0	0.0
60-61	0.0828133566561366	0.0	0.0	0.0	0.0
62-63	0.09017570425293059	0.0	0.0	0.0	0.0
64-65	0.1007753961830666	0.0	0.0	0.0	0.0
66-67	0.11234977242861743	0.0	0.0	0.0	0.0
68-69	0.1275618097799128	0.0	0.0	0.0	0.0
70-71	0.1493703713373191	0.0	0.0	0.0	0.0
72-73	0.17514729046462854	0.0	0.0	0.0	0.0
74-75	0.20919162119590284	0.0	0.0	0.0	0.0
76-77	0.25018057767450763	0.0	0.0	3.481015412195738E-5	0.0
78-79	0.3030223916316389	0.0	0.0	3.481015412195738E-5	0.0
80-81	0.36874396261389447	0.0	0.0	3.481015412195738E-5	0.0
82-83	0.45289751020372643	0.0	0.0	3.481015412195738E-5	0.0
84-85	0.5587551888885989	0.0	0.0	3.481015412195738E-5	0.0
86-87	0.6873438982151094	0.0	0.0	3.481015412195738E-5	0.0
88	0.8014689885039467	0.0	0.0	3.481015412195738E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	2760	0.0	85.91367	1
AACGAGT	63160	0.0	66.78628	1
ACGAGTG	67200	0.0	66.31576	2
GTGGAAT	68460	0.0	66.100945	6
TGGAATC	69145	0.0	65.452896	7
CGAGTGG	68540	0.0	65.373535	3
GGAATCA	69485	0.0	65.20026	8
GAATCAC	69540	0.0	65.16896	9
AGTGGAA	69715	0.0	64.486305	5
GAGTGGA	70405	0.0	63.94887	4
ATCTGCG	1070	0.0	50.07602	94
ACGTGTG	930	0.0	44.97727	2
AACGTGT	925	0.0	42.809574	1
ATCGAGT	465	0.0	42.579414	1
AGTACTT	28880	0.0	40.92117	20-21
GAGTACT	28505	0.0	40.676323	20-21
CGTGTGG	1035	0.0	40.412956	3
GTACTTT	28910	0.0	39.422318	22-23
TACTTTT	30695	0.0	38.186275	22-23
CATGGGG	12845	0.0	37.190895	24-25
>>END_MODULE