FastQCFastQC Report
Thu 26 May 2016
SRR2132661_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132661_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5194116
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2976675.730850061877709No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA727971.401528190745066No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG249800.4809288048245361No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT160670.3093307889157655No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70740.13619256866808518No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT62540.12040547419426134No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT40200.085.7091141
AACGAGT875550.072.422561
ACGAGTG931550.071.754092
CGAGTGG952150.070.680483
GTGGAAT967250.070.64136
TGGAATC976350.069.944397
GAATCAC977400.069.8980949
GGAATCA983150.069.446278
AGTGGAA981750.068.903855
GAGTGGA985200.068.6624154
ATCGAGT8450.045.1818471
AACGTGT15300.042.8212241
AGTACTT463000.040.92826520-21
GAGTACT454400.040.87543520-21
GTACTTT457400.039.88712322-23
TACTTTT495600.037.78469522-23
CATGGGG197300.036.95622624-25
TCGAGTG10950.036.9127582
ACGTGTG18500.036.8373572
ACTTTTT507800.035.6682624-25
Produced by FastQC (version 0.11.5)