##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132661_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5194116
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.464523703359724	33.0	30.0	34.0	16.0	34.0
2	31.061764311771242	33.0	31.0	34.0	25.0	34.0
3	31.860064542262823	34.0	31.0	34.0	28.0	34.0
4	35.935157974908535	37.0	35.0	37.0	35.0	37.0
5	35.69816557812725	37.0	35.0	37.0	35.0	37.0
6	36.1459680530816	37.0	36.0	37.0	35.0	37.0
7	36.21217469921734	37.0	36.0	37.0	35.0	37.0
8	36.34245769636258	37.0	37.0	37.0	35.0	37.0
9	38.258577012912305	39.0	39.0	39.0	37.0	39.0
10-11	38.299142433476646	39.0	39.0	39.0	37.0	39.0
12-13	38.264093543540426	39.0	39.0	39.0	37.0	39.0
14-15	39.796797664896204	41.0	40.0	41.0	38.0	41.0
16-17	39.72174783928584	41.0	40.0	41.0	37.5	41.0
18-19	39.6586958204245	41.0	40.0	41.0	37.0	41.0
20-21	39.63208532501007	41.0	40.0	41.0	37.0	41.0
22-23	39.41030225354998	41.0	39.5	41.0	37.0	41.0
24-25	39.36539855867678	41.0	39.0	41.0	36.0	41.0
26-27	39.308109695663326	41.0	39.0	41.0	36.0	41.0
28-29	39.20666221162561	41.0	39.0	41.0	36.0	41.0
30-31	39.09152760546742	41.0	39.0	41.0	35.5	41.0
32-33	38.98941648588519	41.0	39.0	41.0	35.0	41.0
34-35	38.920166203450215	41.0	39.0	41.0	35.0	41.0
36-37	38.801365044600466	40.5	39.0	41.0	35.0	41.0
38-39	38.457651311599506	40.0	38.0	41.0	35.0	41.0
40-41	38.28880862499028	40.0	38.0	41.0	35.0	41.0
42-43	38.17592223585304	40.0	37.5	41.0	35.0	41.0
44-45	38.0315703384368	40.0	37.0	41.0	34.0	41.0
46-47	37.93987446564536	40.0	37.0	41.0	34.0	41.0
48-49	37.8097188241464	40.0	37.0	41.0	34.0	41.0
50-51	37.611486632181496	40.0	36.5	41.0	33.0	41.0
52-53	37.19997868742246	40.0	36.0	41.0	33.0	41.0
54-55	36.45738822544587	40.0	35.5	41.0	30.5	41.0
56-57	35.90782339092927	40.0	35.0	41.0	28.5	41.0
58-59	35.591928251121075	39.5	35.0	41.0	27.5	41.0
60-61	35.35914350006816	39.0	35.0	41.0	27.0	41.0
62-63	35.0979367807727	39.0	35.0	41.0	26.0	41.0
64-65	34.82701079452211	38.5	35.0	41.0	26.0	41.0
66-67	34.52013374364377	38.0	35.0	40.0	26.0	41.0
68-69	34.21689321147237	37.0	35.0	40.0	25.0	41.0
70-71	33.894158120457845	37.0	35.0	39.5	24.5	41.0
72-73	33.54761378067028	36.0	34.0	39.0	24.0	41.0
74-75	33.356479524138464	36.0	34.0	39.0	24.0	41.0
76-77	33.041037878245305	35.5	34.0	38.5	23.5	40.0
78-79	32.68764877411286	35.0	34.0	37.0	22.0	39.0
80-81	32.36159377264582	35.0	34.0	37.0	20.5	39.0
82-83	32.04074889740622	35.0	34.0	36.5	19.5	39.0
84-85	31.716000278006884	35.0	34.0	36.0	17.5	37.0
86-87	31.481387978243074	35.0	34.0	36.0	13.0	37.0
88-89	31.264495440610105	35.0	34.0	35.5	7.5	37.0
90-91	31.075436513162202	35.0	34.0	35.0	2.0	36.0
92-93	30.91019655702722	35.0	33.5	35.0	2.0	36.0
94-95	30.738394464043544	35.0	33.0	35.0	2.0	36.0
96-97	30.562121350389553	35.0	33.0	35.0	2.0	36.0
98-99	30.433104304948138	35.0	33.0	35.0	2.0	35.5
100	30.341226688044703	35.0	33.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	3.4232063230682073
1101	2	2.1142246283069603
1101	3	1.3522729908255222
1101	4	0.6052211563180876
1101	5	0.8528155476108168
1101	6	0.4148493307691723
1101	7	0.23339580725528464
1101	8	0.18595764873243326
1101	9	0.21953613415369233
1101	10-11	0.15981064596388705
1101	12-13	0.20262808411083455
1101	14-15	0.2620632988316842
1101	16-17	0.23551587319318656
1101	18-19	0.13768548611646736
1101	20-21	0.22207159779828345
1101	22-23	0.17824507163739867
1101	24-25	0.22705233488809995
1101	26-27	0.23413588245417571
1101	28-29	0.24666460336246132
1101	30-31	0.29405582397886576
1101	32-33	0.30935517138043167
1101	34-35	0.25323388306480865
1101	36-37	0.28571751712387794
1101	38-39	0.28120230247785827
1101	40-41	0.2923511262324112
1101	42-43	0.3185038941589369
1101	44-45	0.3790231564209563
1101	46-47	0.3451632071356414
1101	48-49	0.38909842125769245
1101	50-51	0.32003357214968986
1101	52-53	0.3849868696303602
1101	54-55	0.2872090753165324
1101	56-57	0.2487946346088279
1101	58-59	0.21465707808817314
1101	60-61	0.27225408492826375
1101	62-63	0.2793351595778333
1101	64-65	0.28314300697636696
1101	66-67	0.2534917879230534
1101	68-69	0.22307755221479653
1101	70-71	0.24988473410579104
1101	72-73	0.3069384455700188
1101	74-75	0.3955418674403077
1101	76-77	0.3509476950094097
1101	78-79	0.2599010841755103
1101	80-81	0.30235574617550043
1101	82-83	0.25316584699073985
1101	84-85	0.31669164198711286
1101	86-87	0.23195504079389195
1101	88-89	0.27270516559191904
1101	90-91	0.28798872332944114
1101	92-93	0.3334147778614813
1101	94-95	0.30466493781718995
1101	96-97	0.282192137136942
1101	98-99	0.33015853612088364
1101	100	0.36704688405426467
1102	1	3.4608207519917826
1102	2	2.1194391467754095
1102	3	1.3591690409132546
1102	4	0.6516668595090991
1102	5	0.8944509040629498
1102	6	0.45068913153181
1102	7	0.3031465883514102
1102	8	0.24285142073523502
1102	9	0.22540652583003862
1102	10-11	0.21401439429254765
1102	12-13	0.20904562873707988
1102	14-15	0.2415566957935411
1102	16-17	0.32785691578117593
1102	18-19	0.22388474894971466
1102	20-21	0.235515303449624
1102	22-23	0.21088553530481846
1102	24-25	0.20134161723393618
1102	26-27	0.25354476246511126
1102	28-29	0.23886908641834736
1102	30-31	0.2677103995752361
1102	32-33	0.2165880910350637
1102	34-35	0.263921177700837
1102	36-37	0.2518622691516512
1102	38-39	0.1701453661397494
1102	40-41	0.203378877970664
1102	42-43	0.1924862449201541
1102	44-45	0.26204738771079406
1102	46-47	0.21502944408732816
1102	48-49	0.24867842311603994
1102	50-51	0.22175751740533656
1102	52-53	0.18662896526759454
1102	54-55	0.06201663095292531
1102	56-57	-0.0036533739323800773
1102	58-59	-0.1042882295770653
1102	60-61	-0.06391271967913781
1102	62-63	-0.06094774090142607
1102	64-65	-0.06002996711552555
1102	66-67	-0.10548157964971949
1102	68-69	-0.1128994000232737
1102	70-71	-0.08704790166717657
1102	72-73	-0.13131840330397893
1102	74-75	-0.10805410756031364
1102	76-77	-0.08564382893614209
1102	78-79	-0.06439153457818492
1102	80-81	-0.037236885600954395
1102	82-83	0.01110009392581901
1102	84-85	0.011725470651441583
1102	86-87	0.022093067627942276
1102	88-89	0.020597578277588013
1102	90-91	0.05612517631174185
1102	92-93	-0.007425347573104091
1102	94-95	-0.02705387008787241
1102	96-97	-0.007169098611122138
1102	98-99	0.04348016691398726
1102	100	-0.004915953384823979
1103	1	3.3951867396058972
1103	2	2.101825208220884
1103	3	1.370944347869571
1103	4	0.6100502163713628
1103	5	0.8931076731282559
1103	6	0.466114525965466
1103	7	0.3092325814228829
1103	8	0.24208959139160413
1103	9	0.20979389504937984
1103	10-11	0.14668650835849917
1103	12-13	0.2083328736472012
1103	14-15	0.2510702924256947
1103	16-17	0.29916929592700825
1103	18-19	0.19007400221187964
1103	20-21	0.20563109931015333
1103	22-23	0.23395331512430317
1103	24-25	0.22021386260102105
1103	26-27	0.24160966303475817
1103	28-29	0.21244854520317347
1103	30-31	0.2763201814828591
1103	32-33	0.27672513232893436
1103	34-35	0.32855689302093083
1103	36-37	0.3236466158264264
1103	38-39	0.3138598419709382
1103	40-41	0.2990010303087729
1103	42-43	0.3049695671371495
1103	44-45	0.3505994170254567
1103	46-47	0.27862328733725406
1103	48-49	0.2792231394765352
1103	50-51	0.3032793325347143
1103	52-53	0.20148175690915338
1103	54-55	0.23017718179963964
1103	56-57	0.16218704361253344
1103	58-59	0.13894280986224317
1103	60-61	0.07994861229666839
1103	62-63	0.14646854677530285
1103	64-65	0.09406693437171043
1103	66-67	0.10169656434314334
1103	68-69	0.13405219584580408
1103	70-71	0.09867287839872319
1103	72-73	0.0836442162032256
1103	74-75	0.12981445671399428
1103	76-77	0.1909604003008596
1103	78-79	0.1885032048136992
1103	80-81	0.1872869906994552
1103	82-83	0.2081534390013573
1103	84-85	0.2059033368012848
1103	86-87	0.18149894444004389
1103	88-89	0.17798892293047075
1103	90-91	0.2178011694434403
1103	92-93	0.17314439037195228
1103	94-95	0.21849800969958366
1103	96-97	0.2182723667240971
1103	98-99	0.250588784682904
1103	100	0.33484207440628566
1104	1	2.579424949837474
1104	2	1.8113488376654239
1104	3	1.2234596994130307
1104	4	0.5544586478200273
1104	5	0.8443287263501702
1104	6	0.33465125583395405
1104	7	0.20013085198444003
1104	8	0.17067387419289304
1104	9	0.16861954152752645
1104	10-11	0.13310950890620177
1104	12-13	0.13074461358083056
1104	14-15	0.12607883975965706
1104	16-17	0.19330270774059954
1104	18-19	0.15696491902203036
1104	20-21	0.16511320333234636
1104	22-23	0.17513115017872138
1104	24-25	0.20459305775129621
1104	26-27	0.2635597568946437
1104	28-29	0.28247557681677904
1104	30-31	0.28555388330126164
1104	32-33	0.27289064438733845
1104	34-35	0.25604328395879605
1104	36-37	0.27220223858298453
1104	38-39	0.2853281851927676
1104	40-41	0.29214612420767594
1104	42-43	0.2504158554504059
1104	44-45	0.2646092241425464
1104	46-47	0.304153682960262
1104	48-49	0.23027485815739368
1104	50-51	0.25210228725652684
1104	52-53	0.24579010715922323
1104	54-55	0.09128749398763603
1104	56-57	0.06416748683220419
1104	58-59	0.027548971437418857
1104	60-61	0.03585586838508448
1104	62-63	-0.06234219171177813
1104	64-65	0.015670229856667106
1104	66-67	0.01067017166795381
1104	68-69	-0.03673979536015537
1104	70-71	-0.10364536780753753
1104	72-73	-0.06788569869388539
1104	74-75	-0.025909933162431287
1104	76-77	0.008294436457859433
1104	78-79	0.0838790521188244
1104	80-81	0.0157014158722788
1104	82-83	0.07930034072464309
1104	84-85	-0.008158881958841846
1104	86-87	0.007491912258760891
1104	88-89	-0.00532822470649208
1104	90-91	-0.01570215014641363
1104	92-93	-0.05325280395870635
1104	94-95	-0.07764885986668091
1104	96-97	-0.08418890529927481
1104	98-99	-0.06805339017390111
1104	100	-0.06349490210219955
1105	1	3.358540549373892
1105	2	2.0434858575366484
1105	3	1.3250596926376126
1105	4	0.5846629257141274
1105	5	0.8593243465971483
1105	6	0.338674010531399
1105	7	0.18968020256348694
1105	8	0.12241198186710278
1105	9	0.117574940219626
1105	10-11	0.10068307585224545
1105	12-13	0.11062278089278976
1105	14-15	0.13097024742637586
1105	16-17	0.09789326870416204
1105	18-19	0.08355171708363685
1105	20-21	0.08845164969718411
1105	22-23	0.061340808104056066
1105	24-25	0.008382180986906462
1105	26-27	0.02960775143574068
1105	28-29	0.04518153877420872
1105	30-31	0.09347242220480467
1105	32-33	0.08569778739344258
1105	34-35	0.07374929127537655
1105	36-37	-0.010222872192947818
1105	38-39	0.0745871216748526
1105	40-41	0.038918217607104566
1105	42-43	0.06349833356276946
1105	44-45	0.04519513825292165
1105	46-47	0.06718229749175464
1105	48-49	0.029983941929522473
1105	50-51	0.15920145231662275
1105	52-53	0.09168237015570213
1105	54-55	0.16532531328137878
1105	56-57	0.11926902958251162
1105	58-59	0.13910083072936885
1105	60-61	0.08654014056426718
1105	62-63	0.12770696991542252
1105	64-65	0.12363619669680759
1105	66-67	0.1671414471530568
1105	68-69	0.10055526033436024
1105	70-71	0.13397467034838684
1105	72-73	0.19042934619544383
1105	74-75	0.1668602339289933
1105	76-77	0.14743559311770582
1105	78-79	0.10662432623860951
1105	80-81	0.09770607738767723
1105	82-83	0.06914687747264026
1105	84-85	0.08427889624050167
1105	86-87	0.007780989013788542
1105	88-89	0.061347895411120845
1105	90-91	0.10174147034586767
1105	92-93	0.09509708178475762
1105	94-95	0.05822752541621057
1105	96-97	0.09291350586966729
1105	98-99	0.10144708566207328
1105	100	0.04758943985308761
1106	1	1.9764687482712588
1106	2	1.4862981065605787
1106	3	1.0789334142730205
1106	4	0.470437360824306
1106	5	0.6938698813159334
1106	6	0.24397655187409129
1106	7	0.17645538512834946
1106	8	0.11116057604549923
1106	9	0.14582157304474208
1106	10-11	0.15671441541381625
1106	12-13	0.07132190237086888
1106	14-15	0.1351210115671293
1106	16-17	0.15256690682999619
1106	18-19	0.10779492851179384
1106	20-21	0.12832082136667822
1106	22-23	0.04817359908742702
1106	24-25	0.061323557890830216
1106	26-27	0.02489718023598897
1106	28-29	0.07240644653365536
1106	30-31	0.10343569635308825
1106	32-33	0.05135887034313669
1106	34-35	0.015015366877086933
1106	36-37	0.03764267609452787
1106	38-39	0.05885409932352559
1106	40-41	0.07081551724966317
1106	42-43	0.0654461575987284
1106	44-45	0.06545017899762229
1106	46-47	0.04150242359405354
1106	48-49	0.03526344475400123
1106	50-51	0.1220826623675535
1106	52-53	0.08562422425259086
1106	54-55	0.10333950357323118
1106	56-57	0.16347970033621095
1106	58-59	0.06484462992827389
1106	60-61	0.06498287315720574
1106	62-63	0.09897045540125049
1106	64-65	0.05303107489460501
1106	66-67	0.061325442493441074
1106	68-69	0.1262654740192417
1106	70-71	0.12549343095660248
1106	72-73	0.09343016936220039
1106	74-75	0.09134635438962135
1106	76-77	0.08960349747636087
1106	78-79	0.04300911478419067
1106	80-81	0.06902323536331068
1106	82-83	-0.01729268756246327
1106	84-85	-0.0028324518129778653
1106	86-87	-0.013027773052098013
1106	88-89	0.006740966023553341
1106	90-91	0.059317210141248466
1106	92-93	0.045698121535892255
1106	94-95	0.05432803031746758
1106	96-97	0.08840158188071001
1106	98-99	0.03215961095748554
1106	100	0.043907026982346054
1107	1	2.8871949234684564
1107	2	1.860857494687206
1107	3	1.2096988577742103
1107	4	0.5067202443367833
1107	5	0.8285573112900408
1107	6	0.36026766595845316
1107	7	0.17370141595699096
1107	8	0.1409659155414431
1107	9	0.06053204306618909
1107	10-11	0.09828329552115633
1107	12-13	0.0857574961577825
1107	14-15	0.12123302598366337
1107	16-17	0.17690922869130077
1107	18-19	0.16872255580756956
1107	20-21	0.14365594957182992
1107	22-23	0.10938047004028562
1107	24-25	0.13623502370354856
1107	26-27	0.1903725251101136
1107	28-29	0.15892433777164428
1107	30-31	0.1746864005763058
1107	32-33	0.15108199815863088
1107	34-35	0.16844799375142117
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2216	88-89	-0.32575535052282234
2216	90-91	-0.35636540131569205
2216	92-93	-0.3179961954782229
2216	94-95	-0.3123594209885283
2216	96-97	-0.39283049621872834
2216	98-99	-0.363125956474768
2216	100	-0.417580665483019
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	572.0
3	147.0
4	160.0
5	445.0
6	1374.0
7	2599.0
8	9461.0
9	12754.0
10	3031.0
11	6306.0
12	5475.0
13	7765.0
14	8021.0
15	8584.0
16	10856.0
17	13831.0
18	19543.0
19	33823.0
20	77182.0
21	106986.0
22	49502.0
23	27591.0
24	24523.0
25	25580.0
26	26903.0
27	29962.0
28	34881.0
29	42281.0
30	50072.0
31	61084.0
32	78502.0
33	108158.0
34	162413.0
35	321580.0
36	509984.0
37	1020466.0
38	1603784.0
39	682850.0
40	5085.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	14.557891566032415	39.85591312306664	26.80104734283099	18.785147968069946
2	17.35219263767845	37.59539056391738	26.472625071692736	18.579791726711438
3	18.6182000906799	26.360684585091338	21.713561265244476	33.30755405898429
4	29.92095391314326	26.846346852442426	23.965773906936015	19.266925327478297
5	17.758701052518497	36.70576670164858	24.38410562111375	21.15142662471918
6	32.81672954550881	23.994766385656384	25.40786536149751	17.7806387073373
7	19.35975245835865	24.12793630330936	37.50975141872072	19.002559819611267
8	31.981110933987612	24.081268111840398	25.273078229288682	18.664542724883308
9	31.850597868819257	25.787429468267554	24.866291010828405	17.495681652084784
10-11	17.57150783694473	39.38314046124499	24.98763986018025	18.057711841630027
12-13	17.479856052502484	25.413978047467555	31.81353670191424	25.292629198115712
14-15	17.709073756762482	32.21378102077357	24.9182341502859	25.158911072178046
16-17	24.921495280860675	25.174676068461714	31.753862953926028	18.149965696751583
18-19	17.947093575535693	25.294419799026358	31.875917625219625	24.882569000218325
20-21	24.977409479807246	32.282183056229044	24.900967029247685	17.83944043471602
22-23	24.724739448762683	25.45148352287298	31.757389504943024	18.06638752342131
24-25	17.85571404258203	32.23252041348326	24.740427437508135	25.171338106426578
26-27	18.125143912842915	28.329401961758265	35.469654509063716	18.0757996163351
28-29	24.417956780326048	25.26686928054745	32.2652593819622	18.0499145571643
30-31	22.5179029501844	26.41882661072644	32.547049392042844	18.51622104704631
32-33	19.690744266275942	27.355437128804706	33.40586092088248	19.547957684036867
34-35	19.68658999479206	27.222378920272355	33.52338614111103	19.567644943824554
36-37	19.733429082721504	27.199609558124916	33.73499624093807	19.331965118215518
38-39	20.165890064741696	27.112181555908844	33.4107288025217	19.311199576827757
40-41	19.37012182343654	27.982175213469933	33.27383651749754	19.373866445595983
42-43	19.4662637994271	27.32632390799448	33.581390352113836	19.626021940464582
44-45	19.439041886838805	27.712299977551204	33.13628384214067	19.712374293469324
46-47	19.852789135868264	27.29159503015568	33.37860311595238	19.477012718023676
48-49	19.44974275159814	27.54245421694657	33.268524666986394	19.739278364468895
50-51	19.811385051502924	27.895867482827548	32.75921752366254	19.53352994200699
52-53	19.956302246294033	27.745233836504713	32.5482902341768	19.75017368302446
54-55	20.035032058973844	28.43477488253739	31.208836103351505	20.32135695513727
56-57	20.389879163777568	28.996544442329864	30.21852789989558	20.395048493996985
58-59	21.091128922020687	29.231286832869024	29.320503959322842	20.357080285787447
60-61	20.80293646656549	29.643651156936446	29.063558463399335	20.489853913098727
62-63	20.349282537538475	29.74916080336851	28.969632782705858	20.931923876387163
64-65	20.577194984740284	29.63916721922008	29.288594219405677	20.495043576633957
66-67	20.55601242984723	29.237046458676613	29.640022098105153	20.56691901337101
68-69	20.807040292331543	29.34063199561426	29.382246499756214	20.470081212297984
70-71	19.98134391217584	29.92012922565684	29.460619578495635	20.637907283671687
72-73	20.020700364249016	29.3442583972254	29.69938169405874	20.935659544466844
74-75	20.166650542610586	29.688950112170488	29.219446478000773	20.924952867218156
76-77	20.57084295691488	29.31920923833024	29.39750066205492	20.712447142699958
78-79	20.42093723695995	29.60148253510345	29.00044204204944	20.977138185887153
80-81	20.703665700455748	29.91080767127381	28.729954877747893	20.65557175052255
82-83	20.61360768818393	29.87983193625152	28.827975122930333	20.678585252634214
84-85	20.482812063803777	30.18700243547906	28.367372281745457	20.962813218971707
86-87	20.48429337012596	30.238059114396915	28.52927936687516	20.748368148601966
88-89	21.19451577424859	29.929914155550048	28.227455302886266	20.648114767315093
90-91	20.78940642416757	30.361447870844703	28.09361554245579	20.75553016253193
92-93	20.4543929552447	30.465522647397663	28.015777380741845	21.064307016615793
94-95	20.686904564670066	30.588015236906287	27.879699332083323	20.845380866340324
96-97	20.6904564973631	30.18989854416186	28.077637512938047	21.04200744553699
98-99	21.08996602580029	30.264790443982616	27.566867603430325	21.07837592678677
100	20.551022816778243	30.864982871388026	27.511344468487415	21.072649843346312
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	74853.0
1	41844.0
2	6234.5
3	2996.5
4	2306.5
5	2187.5
6	2198.0
7	2484.5
8	2445.0
9	2235.0
10	2250.5
11	2941.5
12	23044.0
13	26279.5
14	9543.5
15	9587.0
16	11172.0
17	13190.0
18	15540.0
19	18363.0
20	21505.5
21	25560.0
22	30730.5
23	36746.5
24	43176.0
25	50052.0
26	59161.5
27	68943.0
28	76675.5
29	83953.5
30	92133.0
31	100310.5
32	108945.5
33	119074.0
34	129771.0
35	138751.0
36	146950.0
37	153578.0
38	159126.0
39	163877.5
40	166973.0
41	169984.0
42	171376.5
43	171249.5
44	172346.5
45	173863.5
46	172737.0
47	172294.5
48	170342.0
49	170822.5
50	192340.0
51	185172.5
52	146211.0
53	128726.5
54	120010.5
55	112099.0
56	101972.5
57	88409.5
58	77348.0
59	64819.5
60	51344.0
61	41322.5
62	32548.5
63	24526.5
64	18178.0
65	13598.0
66	10018.5
67	7409.5
68	5519.0
69	4152.5
70	3232.5
71	2548.5
72	2043.5
73	1543.5
74	1115.0
75	884.5
76	648.5
77	423.5
78	268.5
79	167.0
80	97.5
81	54.0
82	32.5
83	19.0
84	11.5
85	7.5
86	4.0
87	2.5
88	1.5
89	1.5
90	1.0
91	0.0
92	0.0
93	0.5
94	1.0
95	1.0
96	0.5
97	0.0
98	0.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.29448706959952375
2	0.0014439415677277904
3	4.043036389637813E-4
4	0.003542469979492179
5	5.7757662709111616E-5
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	3.0804086778192866E-4
16-17	2.8878831354555808E-5
18-19	3.850510847274108E-5
20-21	2.406569279546317E-4
22-23	0.0024643269422554293
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	9.62627711818527E-6
34-35	0.0023295590626008352
36-37	1.925255423637054E-5
38-39	2.599094821910023E-4
40-41	9.62627711818527E-6
42-43	2.7916203642737285E-4
44-45	0.0014246890134914198
46-47	0.0013476787965459377
48-49	0.0020985284117643888
50-51	8.086072779275626E-4
52-53	5.294452415001898E-4
54-55	3.176671449001139E-4
56-57	3.7542480760922555E-4
58-59	8.856174948730448E-4
60-61	0.002281427677009909
62-63	2.214043737182612E-4
64-65	9.241226033457859E-4
66-67	1.0588904830003798E-4
68-69	2.599094821910023E-4
70-71	0.0019445079778734244
72-73	8.663649406366743E-5
74-75	2.599094821910023E-4
76-77	0.0011647795313004176
78-79	7.701021694548216E-4
80-81	0.0022236700143007976
82-83	2.984145906637434E-4
84-85	0.0012706685796004555
86-87	0.006199322464111314
88-89	0.0024739532193736144
90-91	0.004110420329465111
92-93	0.006767272814084245
94-95	0.005381088909065566
96-97	0.002541337159200911
98-99	5.294452415001898E-4
100	0.003369196991364845
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5194116.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.24956785644588
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.98544280860943	63.401743456279
2	8.424177613527789	12.004380290196009
3	1.31372121085667	2.808062056721537
4	0.3665058280233382	1.0445352745412688
5	0.15381534716936812	0.5479638507753336
6	0.11569745500012636	0.4946036202509758
7	0.08562176911174188	0.4270359833821197
8	0.06770870876200843	0.38593729915288405
9	0.0525939902465597	0.33725691692221577
>10	0.4031595663138472	5.777420625505376
>50	0.018347755099366977	0.897240358473072
>100	0.010617497333518742	1.4331745795115587
>500	9.984036609641442E-4	0.5237517087627244
>1k	0.001430143273255019	1.7489962626099542
>5k	5.3967670688222204E-5	0.25624234303126076
>10k+	1.0793534137644441E-4	7.911655373884839
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	297667	5.730850061877709	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	72797	1.401528190745066	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	24980	0.4809288048245361	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	16067	0.3093307889157655	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7074	0.13619256866808518	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6254	0.12040547419426134	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0018289926524552012	0.0	0.0	0.0	0.0
2	0.0019060028694006833	0.0	0.0	1.9252554236370538E-5	0.0
3	0.0019252554236370539	0.0	0.0	1.9252554236370538E-5	0.0
4	0.0019252554236370539	0.0	0.0	1.9252554236370538E-5	0.0
5	0.0019445079778734244	0.0	0.0	1.9252554236370538E-5	0.0
6	0.0019445079778734244	0.0	0.0	3.8505108472741075E-5	0.0
7	0.0019445079778734244	0.0	0.0	7.701021694548215E-5	0.0
8	0.001963760532109795	0.0	0.0	1.347678796545938E-4	0.0
9	0.0019830130863461655	0.0	0.0	1.7327298812733484E-4	0.0
10-11	0.0019926393634643507	0.0	0.0	1.828992652455201E-4	0.0
12-13	0.0020215181948189066	0.0	0.0	2.0215181948189065E-4	0.0
14-15	0.0020600233032916477	0.0	0.0	2.3103065083644647E-4	0.0
16-17	0.0020600233032916477	0.0	0.0	2.599094821910023E-4	0.0
18-19	0.002069649580409833	0.0	0.0	2.887883135455581E-4	0.0
20-21	0.002117780966000759	0.0	0.0	3.465459762546697E-4	0.0
22-23	0.002146659797355315	0.0	0.0	4.043036389637813E-4	0.0
24-25	0.0021659123515916858	0.0	0.0	4.6206130167289293E-4	0.0
26-27	0.0022332962914189828	0.0	0.0	4.909401330274488E-4	0.0
28-29	0.002339185339719021	0.0	0.0	5.101926872638193E-4	0.0
30-31	0.0024258218337826877	0.0	0.0	5.583240728547457E-4	0.0
32-33	0.0025220846049645402	0.0	0.0	6.064554584456719E-4	0.0
34-35	0.0026568524846191342	0.0	0.0	6.257080126820425E-4	0.0
36-37	0.002993772183755619	0.0	0.0	6.545868440365983E-4	0.0
38-39	0.0033403181600102885	0.0	0.0	7.027182296275246E-4	0.0
40-41	0.0038216320159195523	0.0	0.0	7.123445067457099E-4	0.0
42-43	0.004408834920128854	0.0	0.0	7.123445067457099E-4	0.0
44-45	0.005477351680247418	0.0	0.0	7.123445067457099E-4	0.0
46-47	0.0065073633318932424	0.0	0.0	7.123445067457099E-4	0.0
48-49	0.007941678622502847	0.0	0.0	7.123445067457099E-4	0.0
50-51	0.009501135515648861	0.0	0.0	7.123445067457099E-4	0.0
52-53	0.011955836180786106	0.0	0.0	7.123445067457099E-4	0.0
54-55	0.01514213390690543	0.0	0.0	7.123445067457099E-4	0.0
56-57	0.019002271031297722	0.0	0.0	7.412233381002657E-4	0.0
58-59	0.02395017747004495	0.0	0.0	7.50849615218451E-4	0.0
60-61	0.030871470718020162	0.0	0.0	7.893547236911921E-4	0.0
62-63	0.03993942376335068	0.0	0.0	8.278598321639332E-4	0.0
64-65	0.05147170375093664	0.0	0.0	8.374861092821184E-4	0.0
66-67	0.06592074570533273	0.0	0.0	8.56738663518489E-4	0.0
68-69	0.08538507803830334	0.0	0.0	8.759912177548595E-4	0.0
70-71	0.11054816642523964	0.0	0.0	8.9524377199123E-4	0.0
72-73	0.1427961947711603	0.0	0.0	9.048700491094153E-4	0.0
74-75	0.18388114551157503	0.0	0.0	9.048700491094153E-4	0.0
76-77	0.23645024485398478	0.0	0.0	9.144963262276006E-4	0.0
78-79	0.302034070860181	0.0	0.0	9.433751575821564E-4	0.0
80-81	0.38398256796729224	0.0	0.0	9.433751575821564E-4	0.0
82-83	0.4865312981073199	0.0	0.0	9.530014347003417E-4	0.0
84-85	0.6166593121909484	0.0	1.9252554236370538E-5	9.722539889367122E-4	0.0
86-87	0.77313444674705	0.0	1.9252554236370538E-5	9.91506543173083E-4	0.0
88	0.9073728811601435	0.0	1.9252554236370538E-5	0.0010011328202912681	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	4020	0.0	85.709114	1
AACGAGT	87555	0.0	72.42256	1
ACGAGTG	93155	0.0	71.75409	2
CGAGTGG	95215	0.0	70.68048	3
GTGGAAT	96725	0.0	70.6413	6
TGGAATC	97635	0.0	69.94439	7
GAATCAC	97740	0.0	69.898094	9
GGAATCA	98315	0.0	69.44627	8
AGTGGAA	98175	0.0	68.90385	5
GAGTGGA	98520	0.0	68.662415	4
ATCGAGT	845	0.0	45.181847	1
AACGTGT	1530	0.0	42.821224	1
AGTACTT	46300	0.0	40.928265	20-21
GAGTACT	45440	0.0	40.875435	20-21
GTACTTT	45740	0.0	39.887123	22-23
TACTTTT	49560	0.0	37.784695	22-23
CATGGGG	19730	0.0	36.956226	24-25
TCGAGTG	1095	0.0	36.912758	2
ACGTGTG	1850	0.0	36.837357	2
ACTTTTT	50780	0.0	35.66826	24-25
>>END_MODULE