FastQCFastQC Report
Thu 26 May 2016
SRR2132660_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132660_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3712075
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2673737.202790891886614No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1644844.4310527131052035No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG549291.4797384212334073No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179220.4828027450953981No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT148440.399884161823239No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAGGTATATCTCGTA122520.3300579864361577TruSeq Adapter, Index 5 (97% over 37bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA92310.24867493248385336No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT74150.199753507135497No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73560.19816409959389292No Hit
GTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGT64110.17270663981735282No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC52680.14191523608763293No Hit
CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT43160.11626920253497033No Hit
TTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTG43140.11621532431322106No Hit
CCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGAT43130.1161883852023464No Hit
CACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCA41220.1110430150252891No Hit
AGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTC41160.1108813803600412No Hit
ACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGA40110.10805277371820343No Hit
CCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATT39110.10535886263073888No Hit
CTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCG39090.10530498440898958No Hit
CCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTC38770.10444293286100091No Hit
ATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTA38410.10347312486951368No Hit
TACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACT38150.10277270798677289No Hit
AACGAGTGGAATCACTGCTGAGTACATGGTAACGAGTGGAATCACTGCTG37580.10123717866691809No Hit
TCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGT37360.1006445182276759No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT42050.086.884611
GTGGAAT1189700.057.0136456
TGGAATC1198900.056.5800557
GGAATCA1207600.056.2541548
GAATCAC1212250.056.0383729
AGTGGAA1200300.055.9071855
AACGTGT11400.055.8259471
ACGAGTG1195500.055.5118522
AACGAGT1130000.055.4230351
CGAGTGG1218100.054.8501133
GAGTGGA1241100.054.1365784
AGTACTT406750.040.91021720-21
GAGTACT400250.040.60582720-21
TGATACG6100.040.064213
ATCGAGT8150.039.9115561
GTACTTT404450.039.38074522-23
TACTTTT423850.038.68153822-23
GATACGG7050.038.6653484
ATCTGCG18350.038.1642494
ATACGGC7300.037.982965
Produced by FastQC (version 0.11.5)