##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132660_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3712075
Sequences flagged as poor quality	0
Sequence length	100
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.485662331714742	31.0	30.0	34.0	16.0	34.0
2	31.06999427543894	33.0	31.0	34.0	25.0	34.0
3	31.847044038711502	34.0	31.0	34.0	28.0	34.0
4	35.91888903106753	37.0	35.0	37.0	35.0	37.0
5	35.672214596957225	37.0	35.0	37.0	35.0	37.0
6	36.11097701420365	37.0	36.0	37.0	35.0	37.0
7	36.153080958762956	37.0	35.0	37.0	35.0	37.0
8	36.30084009617263	37.0	37.0	37.0	35.0	37.0
9	38.21252183751675	39.0	39.0	39.0	37.0	39.0
10-11	38.25335237030502	39.0	39.0	39.0	37.0	39.0
12-13	38.21418519291771	39.0	39.0	39.0	37.0	39.0
14-15	39.731656822666565	41.0	40.0	41.0	37.5	41.0
16-17	39.58277849989561	41.0	39.5	41.0	37.0	41.0
18-19	39.303622232848205	41.0	39.0	41.0	35.5	41.0
20-21	39.17463548015598	41.0	39.0	41.0	35.0	41.0
22-23	38.611472289757074	40.5	39.0	41.0	35.0	41.0
24-25	38.53514220483153	41.0	39.0	41.0	35.0	41.0
26-27	38.468085504737914	41.0	39.0	41.0	35.0	41.0
28-29	38.33884848770566	41.0	38.5	41.0	35.0	41.0
30-31	38.16784870995333	41.0	38.0	41.0	34.5	41.0
32-33	38.03392186310891	40.0	38.0	41.0	34.0	41.0
34-35	37.94651684031169	40.0	38.0	41.0	34.0	41.0
36-37	37.77354983398773	40.0	38.0	41.0	34.0	41.0
38-39	37.349314332280464	40.0	36.0	41.0	33.5	41.0
40-41	37.1555939737209	40.0	35.0	41.0	33.0	41.0
42-43	37.04045648323377	40.0	35.0	41.0	33.0	41.0
44-45	36.87121556003044	40.0	35.0	41.0	33.0	41.0
46-47	36.74849565270098	40.0	35.0	41.0	33.0	41.0
48-49	36.579322885448164	40.0	35.0	41.0	32.5	41.0
50-51	36.31943697258272	39.0	35.0	41.0	31.5	41.0
52-53	35.810400921317594	39.0	35.0	41.0	30.0	41.0
54-55	34.91137530895793	39.0	35.0	41.0	24.5	41.0
56-57	34.28642470855249	39.0	35.0	41.0	17.0	41.0
58-59	33.952578274954035	38.5	35.0	41.0	9.0	41.0
60-61	33.6906645205175	38.0	35.0	40.5	7.0	41.0
62-63	33.43239185630678	37.5	34.5	40.0	4.0	41.0
64-65	33.13182573089175	37.0	34.0	40.0	2.0	41.0
66-67	32.770068762080506	36.0	34.0	40.0	2.0	41.0
68-69	32.45997198332469	36.0	34.0	39.0	2.0	41.0
70-71	32.16118828418068	35.0	33.5	39.0	2.0	41.0
72-73	31.823986988409448	35.0	33.0	38.5	2.0	40.5
74-75	31.60695554373228	35.0	33.0	37.5	2.0	40.0
76-77	31.205317107008884	35.0	33.0	37.0	2.0	39.5
78-79	30.830765676878833	35.0	33.0	36.5	2.0	39.0
80-81	30.52033660419038	35.0	33.0	36.0	2.0	39.0
82-83	30.206566408275695	35.0	33.0	36.0	2.0	37.0
84-85	29.894349521494043	35.0	32.0	35.0	2.0	37.0
86-87	29.699711347426977	35.0	32.0	35.0	2.0	37.0
88-89	29.48546055777429	35.0	32.0	35.0	2.0	36.0
90-91	29.290785881211992	35.0	32.0	35.0	2.0	36.0
92-93	29.142141928705644	35.0	32.0	35.0	2.0	36.0
94-95	28.939857357407917	35.0	31.5	35.0	2.0	35.5
96-97	28.707512779240723	35.0	31.0	35.0	2.0	35.0
98-99	28.561711980496085	35.0	31.0	35.0	2.0	35.0
100	28.478453425644688	35.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	3.3391186427943573
1101	2	2.049179739408409
1101	3	1.3371981555618575
1101	4	0.5835858051210749
1101	5	0.8592614603833866
1101	6	0.41861388823597423
1101	7	0.2720784696180232
1101	8	0.22928106307085727
1101	9	0.2238853166222512
1101	10-11	0.16328439595743305
1101	12-13	0.16943847855081628
1101	14-15	0.3005130700003491
1101	16-17	0.22363331911784456
1101	18-19	0.14472619230080852
1101	20-21	0.16611719629236887
1101	22-23	-0.024257009533243945
1101	24-25	0.02435714191646099
1101	26-27	0.0640636501784897
1101	28-29	0.10770221741525177
1101	30-31	0.16333267087259173
1101	32-33	0.11971190069979087
1101	34-35	0.15172640706297358
1101	36-37	0.18940964900550483
1101	38-39	0.1710200823669723
1101	40-41	0.11267752714700663
1101	42-43	0.15774923935967422
1101	44-45	0.09264819694811166
1101	46-47	0.08220551394470021
1101	48-49	0.13370041844487446
1101	50-51	0.10557108744657029
1101	52-53	0.047509702548012456
1101	54-55	0.054697172632849345
1101	56-57	-0.02312752151735964
1101	58-59	-0.061247509350124574
1101	60-61	-0.03546687970340656
1101	62-63	-0.015859889097988855
1101	64-65	0.028605769402204828
1101	66-67	-0.001364763847220729
1101	68-69	-0.031066527188407633
1101	70-71	-0.033051662105897606
1101	72-73	0.045014812826263295
1101	74-75	0.03233735010737959
1101	76-77	0.05517917864300159
1101	78-79	-0.049510731516701156
1101	80-81	-0.006082260873622403
1101	82-83	-0.01429446788894495
1101	84-85	-0.005296438186253027
1101	86-87	-0.04071757139218235
1101	88-89	0.041944766378911424
1101	90-91	0.03322578551670219
1101	92-93	0.06819890381859395
1101	94-95	0.05672115912119935
1101	96-97	0.027706363112073973
1101	98-99	0.11695096125395921
1101	100	0.11371247585525879
1102	1	3.3087912479231
1102	2	2.018647943155507
1102	3	1.3048627218008235
1102	4	0.6012456308216656
1102	5	0.8568510339635438
1102	6	0.40505687001511603
1102	7	0.28812314516051174
1102	8	0.23392445141983842
1102	9	0.18684422067357787
1102	10-11	0.22169367685054908
1102	12-13	0.20173017530735393
1102	14-15	0.2308857791019605
1102	16-17	0.30275256784795346
1102	18-19	0.21548456679412453
1102	20-21	0.21797597467955399
1102	22-23	0.17077566828258028
1102	24-25	0.1733108829455432
1102	26-27	0.20628258365837127
1102	28-29	0.20216319516414671
1102	30-31	0.2601208010607081
1102	32-33	0.33861837280176843
1102	34-35	0.2829974437558036
1102	36-37	0.26029608006061977
1102	38-39	0.18476953986330358
1102	40-41	0.1779396594252134
1102	42-43	0.14605689197089333
1102	44-45	0.13011831604992352
1102	46-47	0.04231965193140752
1102	48-49	0.1456243876524681
1102	50-51	0.1264933073511898
1102	52-53	0.1986143897619712
1102	54-55	0.06890406106928992
1102	56-57	-0.13631126886065914
1102	58-59	-0.1850336346892476
1102	60-61	-0.14040354345249284
1102	62-63	-0.14934662684379418
1102	64-65	-0.24604060858723642
1102	66-67	-0.16221876253121081
1102	68-69	-0.1527411015456579
1102	70-71	-0.12098925145618722
1102	72-73	-0.15350638730438249
1102	74-75	-0.08615548122391203
1102	76-77	-5.085192966269858E-4
1102	78-79	0.05817980401172562
1102	80-81	0.06442246380388283
1102	82-83	0.0966071754208997
1102	84-85	0.08632028742553643
1102	86-87	0.09040545417358814
1102	88-89	0.08731198443073751
1102	90-91	0.12736614337684316
1102	92-93	0.08656116211538034
1102	94-95	0.13948576666930634
1102	96-97	0.1720773592400704
1102	98-99	0.1816649632448062
1102	100	0.14454408679298325
1103	1	3.3428567532704143
1103	2	2.058008361661102
1103	3	1.3407438505121547
1103	4	0.6161297886682462
1103	5	0.8499026959865574
1103	6	0.43891801948596054
1103	7	0.3117324414951099
1103	8	0.21916104931845126
1103	9	0.25168767406876924
1103	10-11	0.17989766021817388
1103	12-13	0.16152696744408246
1103	14-15	0.25413828627227275
1103	16-17	0.26544225797489673
1103	18-19	0.15730875976704795
1103	20-21	0.17377961011819565
1103	22-23	0.13432529651208114
1103	24-25	0.12757946754053506
1103	26-27	0.16151649968382742
1103	28-29	0.14402585372453558
1103	30-31	0.16658416300545298
1103	32-33	0.12738715960905012
1103	34-35	0.15719472768042664
1103	36-37	0.194498370466043
1103	38-39	0.21109191054390664
1103	40-41	0.19338269963092358
1103	42-43	0.10190792463495768
1103	44-45	0.2007161260599375
1103	46-47	0.16418518290656436
1103	48-49	0.1616528669541637
1103	50-51	0.14969686914550806
1103	52-53	0.1323246064601804
1103	54-55	0.10837535097405748
1103	56-57	0.08047756673479256
1103	58-59	0.05539546025239872
1103	60-61	0.061395026437452316
1103	62-63	0.09786003315709735
1103	64-65	0.0803908709826544
1103	66-67	0.11023632800224448
1103	68-69	0.14971262185176215
1103	70-71	0.18664482195663368
1103	72-73	0.0784272908775101
1103	74-75	0.13292902350366376
1103	76-77	0.09190749591621383
1103	78-79	0.05042535593693387
1103	80-81	0.013803179754457773
1103	82-83	0.05288895121153914
1103	84-85	0.04154693905211815
1103	86-87	0.033564033857377495
1103	88-89	0.07694318638150222
1103	90-91	0.10473170555318845
1103	92-93	0.05061024753947052
1103	94-95	0.11059310876151685
1103	96-97	0.11486640728942632
1103	98-99	0.16685583115707914
1103	100	0.18120881787446308
1104	1	2.4632667434975666
1104	2	1.713874558820038
1104	3	1.1954531147146668
1104	4	0.5286631976351686
1104	5	0.8415856131792765
1104	6	0.37252592823750774
1104	7	0.24374424776269166
1104	8	0.1687509386713586
1104	9	0.16809487162952763
1104	10-11	0.14603530210095528
1104	12-13	0.13715119518390395
1104	14-15	0.07782639672655733
1104	16-17	0.135646323365755
1104	18-19	0.08430260294408498
1104	20-21	-0.009691850724429685
1104	22-23	-0.051141401755295135
1104	24-25	-0.02447728833129048
1104	26-27	0.03495997937090323
1104	28-29	0.056977066087668504
1104	30-31	0.13232058446234873
1104	32-33	0.06100951539052346
1104	34-35	0.09933674423823646
1104	36-37	0.1261272379393219
1104	38-39	0.08215316077070156
1104	40-41	0.07358754068769002
1104	42-43	0.07218357262041764
1104	44-45	0.08439499848791598
1104	46-47	0.1377816800181364
1104	48-49	0.12310090874083102
1104	50-51	0.13108951506571742
1104	52-53	0.09936676023617963
1104	54-55	-0.024701287172817388
1104	56-57	0.06904428143713659
1104	58-59	0.04955571511752055
1104	60-61	0.10392577069259801
1104	62-63	0.049884373190195674
1104	64-65	0.14354462431430193
1104	66-67	0.12164896026993688
1104	68-69	0.13755826325851217
1104	70-71	0.04911940678869087
1104	72-73	0.1454931956379788
1104	74-75	0.1855092159562659
1104	76-77	0.21631270378963308
1104	78-79	0.3244172475708247
1104	80-81	0.387428702109613
1104	82-83	0.30384965347375825
1104	84-85	0.2876170105445688
1104	86-87	0.28092671929484325
1104	88-89	0.2968935827817667
1104	90-91	0.2662340944091106
1104	92-93	0.28592932373414826
1104	94-95	0.3379218673926516
1104	96-97	0.28794971360675703
1104	98-99	0.3188815738471007
1104	100	0.329013437789083
1105	1	3.309579621117198
1105	2	2.0155764706757466
1105	3	1.3332483114109976
1105	4	0.6000470891344989
1105	5	0.8509546524691842
1105	6	0.4271705203267402
1105	7	0.25991506516285057
1105	8	0.1553134919468775
1105	9	0.1429361559106468
1105	10-11	0.0905372251785792
1105	12-13	0.12746408022297828
1105	14-15	0.1483795088881621
1105	16-17	0.1111328676850789
1105	18-19	0.04433125923352321
1105	20-21	0.013538090295270422
1105	22-23	0.026174337684189197
1105	24-25	0.028066507190864343
1105	26-27	0.06910578190648664
1105	28-29	0.028808406554205135
1105	30-31	0.09229134256654703
1105	32-33	0.11005613465818698
1105	34-35	0.12455446774718126
1105	36-37	0.1375846197244215
1105	38-39	0.20004608814632974
1105	40-41	0.1606561267520732
1105	42-43	0.18798790908186902
1105	44-45	0.17095464029291207
1105	46-47	0.20074136900285566
1105	48-49	0.07431673585234222
1105	50-51	0.20412905286861616
1105	52-53	0.15380870867705454
1105	54-55	0.16027457097318631
1105	56-57	0.170704203859529
1105	58-59	0.24885852557426347
1105	60-61	0.18116443400103321
1105	62-63	0.20806993283296293
1105	64-65	0.2291147801713791
1105	66-67	0.274733997832044
1105	68-69	0.24894988356936665
1105	70-71	0.2798586132569625
1105	72-73	0.27449957699179706
1105	74-75	0.25563865781610673
1105	76-77	0.27216076994221794
1105	78-79	0.2733605212276231
1105	80-81	0.3159672045187314
1105	82-83	0.2935515106110458
1105	84-85	0.32426652868294426
1105	86-87	0.34529287377786844
1105	88-89	0.3474021477881628
1105	90-91	0.37306608358042936
1105	92-93	0.3271301095728383
1105	94-95	0.36339534652938354
1105	96-97	0.3378054831990056
1105	98-99	0.385500209530683
1105	100	0.30483074370901875
1106	1	1.8512025421788643
1106	2	1.348738092288528
1106	3	1.0024072510502364
1106	4	0.45790412686201876
1106	5	0.6746721294516433
1106	6	0.21875991671916495
1106	7	0.13107567285880606
1106	8	0.11607981682545443
1106	9	0.14254694267739865
1106	10-11	0.07939722288908513
1106	12-13	0.023297209137595587
1106	14-15	0.04660515444246727
1106	16-17	0.1068523397941803
1106	18-19	0.09825936925985701
1106	20-21	0.0955884917736256
1106	22-23	0.06036132357112223
1106	24-25	0.070412252828568
1106	26-27	0.10430831996925605
1106	28-29	0.10835917598625144
1106	30-31	0.15007969560584655
1106	32-33	0.17415203156573256
1106	34-35	0.1595983768871463
1106	36-37	0.1526421022670661
1106	38-39	0.1113332719105884
1106	40-41	0.13604166044844845
1106	42-43	0.15248364899585454
1106	44-45	0.24477841500713993
1106	46-47	0.2164570798076113
1106	48-49	0.26956686259195095
1106	50-51	0.3690982265263969
1106	52-53	0.26920307326192017
1106	54-55	0.2617643745676901
1106	56-57	0.2068789573305594
1106	58-59	0.1934414296282938
1106	60-61	0.21374387111366389
1106	62-63	0.24373363951663407
1106	64-65	0.24329816828892348
1106	66-67	0.21446236624363735
1106	68-69	0.2502573456701356
1106	70-71	0.31384281577867057
1106	72-73	0.2733908098819491
1106	74-75	0.2667843838296129
1106	76-77	0.25845735696735517
1106	78-79	0.17690923011901916
1106	80-81	0.1938687237586194
1106	82-83	0.2234146318395176
1106	84-85	0.2749018620489885
1106	86-87	0.20521126982686155
1106	88-89	0.2719111340348732
1106	90-91	0.2980068021530933
1106	92-93	0.2630840908317289
1106	94-95	0.2588327208118706
1106	96-97	0.2618377650024719
1106	98-99	0.2907971072722688
1106	100	0.2808777763669532
1107	1	2.797023086641939
1107	2	1.8218419115461941
1107	3	1.1823490287295755
1107	4	0.4904263557643418
1107	5	0.798547499933342
1107	6	0.3341192180513133
1107	7	0.17720947872111736
1107	8	0.1374396033436085
1107	9	0.03996490932613028
1107	10-11	0.08067821941895659
1107	12-13	0.04293353058387339
1107	14-15	0.056953855515146756
1107	16-17	0.11840451311106648
1107	18-19	0.1043094277986043
1107	20-21	0.0861874855400444
1107	22-23	-0.08711149019128328
1107	24-25	-0.022771411169578926
1107	26-27	-0.033902187472023115
1107	28-29	-0.10095850482464641
1107	30-31	0.006441433415545816
1107	32-33	-0.03251505700180246
1107	34-35	-0.030419448424794382
1107	36-37	-0.025676939174154256
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2216	56-57	0.0034878920178158523
2216	58-59	-7.682648044706752E-4
2216	60-61	0.04930803889858737
2216	62-63	0.029238055787743633
2216	64-65	0.03529889265085728
2216	66-67	-0.10460846433578297
2216	68-69	-0.08805288819527846
2216	70-71	-0.004396917976592363
2216	72-73	-0.07761828970613394
2216	74-75	-0.04632498146911246
2216	76-77	-0.058119849332257445
2216	78-79	-0.10646307522123166
2216	80-81	-0.11110659118860511
2216	82-83	-0.12836055559330362
2216	84-85	-0.10304240213949711
2216	86-87	-0.11622358433271174
2216	88-89	-0.17284183184333912
2216	90-91	-0.24111900649518603
2216	92-93	-0.2087338907020495
2216	94-95	-0.2462769515896035
2216	96-97	-0.30572492401593365
2216	98-99	-0.40974641452869065
2216	100	-0.4409782592035576
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	528.0
3	150.0
4	314.0
5	859.0
6	2888.0
7	5779.0
8	22242.0
9	30827.0
10	4664.0
11	8581.0
12	6990.0
13	9452.0
14	9198.0
15	10010.0
16	12175.0
17	15008.0
18	19428.0
19	32148.0
20	67782.0
21	89174.0
22	41744.0
23	24091.0
24	23627.0
25	25365.0
26	26157.0
27	28575.0
28	35216.0
29	45734.0
30	47755.0
31	52441.0
32	66921.0
33	91127.0
34	136320.0
35	342697.0
36	391813.0
37	718216.0
38	929408.0
39	333523.0
40	3148.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	13.437133889856776	44.37319781944258	25.16997777941094	17.0196905112897
2	16.269506878188285	42.56230563164462	23.909624506738105	17.258562983428988
3	17.145761955741087	25.70560437233605	20.1905084661749	36.95812520574796
4	33.81200499253894	26.46886169196207	22.256481046698234	17.462652268800756
5	15.833031390798947	41.51742623734704	22.818854683701165	19.830687688152853
6	37.00334718452618	23.37000195308554	23.884781422789143	15.741869439599146
7	18.42988086178216	23.548150293299567	40.871076150131664	17.15089269478661
8	35.975242957106204	24.175104220685196	23.023511109015846	16.826141713192754
9	35.55631284389459	26.048315295353675	22.894122559484924	15.501249301266812
10-11	15.838540438972812	44.664533987055755	23.187880094017498	16.309045479953934
12-13	15.753224274832808	25.002983506529368	32.60937615753992	26.634416061097905
14-15	15.856430970553342	34.46770102271833	23.01454337582178	26.661324630906545
16-17	25.7804227797691	24.726308698815178	32.589566831161385	16.90370169025433
18-19	16.47237730918691	24.7583090320104	32.96793572328146	25.801377935521234
20-21	25.999154109639782	34.471163624559374	23.528399960668793	16.00128230513205
22-23	25.643906826281842	25.26243817466711	32.625793396201125	16.467861602849915
24-25	15.879043392172843	34.785975498878656	22.761972751089353	26.573008357859152
26-27	16.394981243644054	29.590471636483638	37.27890734966293	16.735639770209385
28-29	26.267464962318922	24.928847073402345	32.152610063104866	16.65107790117387
30-31	23.099856549234595	26.864476067967374	32.87142635857304	17.164241024224996
32-33	18.172102957524157	28.317023270949974	33.882698205545175	19.628175565980694
34-35	18.7538279653927	27.979886268597326	34.023838686053026	19.242447079956946
36-37	19.022616335587106	27.522340276936863	34.70965755392505	18.74538583355098
38-39	20.543677932710843	27.36337875184971	33.91830809179463	18.17463522364482
40-41	17.902911444407778	29.773819225096474	34.24747614204993	18.075793188445815
42-43	17.822533273546394	27.737767911466122	35.17628504092258	19.263413774064905
44-45	17.995601317988232	28.728380038798274	33.68124803173258	19.594770611480918
46-47	19.038047836160565	27.531445441699	34.56229882103418	18.868207901106256
48-49	18.377937224273225	27.819107609681875	34.486738713925185	19.31621645211972
50-51	19.132639007403338	29.130695333012845	33.476780903446254	18.25988475613756
52-53	19.40952788119061	28.0747352517003	33.46080960091334	19.054927266195744
54-55	19.155962714538692	29.59130413582634	30.849324102349	20.403409047285965
56-57	19.279685188066185	30.263607177421147	30.563158032977668	19.893549601535
58-59	21.31598708797111	29.966076627624815	29.152225493107903	19.56571079129617
60-61	20.185883996263463	31.079952343653712	28.80575593835386	19.928407721728963
62-63	19.304301724994875	30.97399320971674	28.3744052171444	21.347299848143987
64-65	19.953685918762858	30.914770452736846	29.205214590551947	19.926329037948346
66-67	20.38456730857301	29.99169196253948	29.886575353684254	19.737165375203254
68-69	20.972180597470278	30.411165745234968	29.17709605226737	19.43955760502739
70-71	18.36293955739775	32.438191692297494	29.28866398473492	19.910204765569837
72-73	18.401918390805992	30.677402457039125	30.11500727559115	20.805671876563732
74-75	18.70100275560462	31.760114502120246	28.731185367952282	20.80769737432285
76-77	19.99527213191156	30.585898695660667	29.32091785295522	20.09791131947255
78-79	19.466300076628848	30.889413217526656	28.795326892954122	20.848959812890374
80-81	20.242311781442567	31.87697341685178	27.99431680192414	19.88639799978152
82-83	20.089402971765878	31.136786519862643	28.575519474449422	20.198291033922054
84-85	19.422662754515763	32.084850194280776	27.3911985225313	21.101288528672157
86-87	19.56244747398973	32.21477206186724	28.03338172337983	20.189398740763203
88-89	21.62675326879481	31.008285302108433	27.364476514650477	20.000484914446275
90-91	20.411394706910198	32.09102047611838	27.24423440718504	20.253350409786382
92-93	19.349819448921497	32.03323804791194	27.16021111416591	21.456731389000655
94-95	20.01481186732845	32.281788636700696	27.089539462190203	20.613860033780647
96-97	20.22684113954247	31.25971011166275	27.85029072077686	20.66315802801792
98-99	21.322346955585072	31.382215467646397	26.72118099654598	20.574256580222553
100	19.6094996815695	33.0165551531322	26.717585014477542	20.656360150820756
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	176053.0
1	98004.5
2	13661.0
3	5943.5
4	4211.0
5	3809.0
6	3850.5
7	4306.0
8	4190.5
9	3693.5
10	3761.5
11	4758.0
12	31972.0
13	35021.5
14	10359.0
15	9577.5
16	10892.0
17	12615.0
18	14329.5
19	16051.0
20	18049.5
21	20301.0
22	23040.0
23	26243.0
24	29131.5
25	32042.0
26	36580.0
27	41582.5
28	45409.5
29	48985.5
30	53093.0
31	57901.0
32	63110.5
33	67967.5
34	73405.5
35	78481.5
36	82845.5
37	86912.0
38	89500.0
39	91511.0
40	93235.5
41	94430.5
42	94953.0
43	95326.0
44	96739.0
45	99114.5
46	102400.5
47	110544.5
48	117556.5
49	132523.5
50	195489.0
51	193208.5
52	118890.0
53	93190.5
54	87806.0
55	84879.5
56	78493.5
57	68229.0
58	61650.5
59	52998.0
60	42626.0
61	35344.0
62	29745.0
63	23770.0
64	17409.0
65	13440.5
66	10006.0
67	6186.0
68	4316.5
69	3309.0
70	2601.0
71	1915.5
72	1351.0
73	938.5
74	679.5
75	540.5
76	396.0
77	266.5
78	169.5
79	107.0
80	61.0
81	35.5
82	22.0
83	16.5
84	12.0
85	9.5
86	8.0
87	5.5
88	1.5
89	1.0
90	1.0
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.29641103695372534
2	0.0013738946546069247
3	2.4245199787181022E-4
4	0.003663719078951799
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	1.8857377612251908E-4
16-17	1.3469555437322791E-5
18-19	0.0
20-21	2.6939110874645586E-4
22-23	0.002842076197275109
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	1.3469555437322791E-5
34-35	0.002249415758032906
36-37	4.040866631196837E-5
38-39	1.616346652478735E-4
40-41	0.0
42-43	1.2122599893590511E-4
44-45	0.0014547119872308613
46-47	0.001360425099169602
48-49	0.001804920428601254
50-51	9.02460214300627E-4
52-53	3.097997750584242E-4
54-55	3.097997750584242E-4
56-57	4.7143444030629773E-4
58-59	8.755211034259813E-4
60-61	0.0022224766471582607
62-63	1.616346652478735E-4
64-65	7.812342153647219E-4
66-67	1.8857377612251908E-4
68-69	2.0204333155984187E-4
70-71	0.0019934942047237733
72-73	1.481651098105507E-4
74-75	1.0775644349858233E-4
76-77	0.0011449122121724373
78-79	7.138864381781079E-4
80-81	0.002168598425408969
82-83	1.616346652478735E-4
84-85	0.001360425099169602
86-87	0.006020891280483288
88-89	0.002155128869971647
90-91	0.0037984146333250274
92-93	0.006586612608850844
94-95	0.005024144178121401
96-97	0.0025322764222166848
98-99	6.330691055541712E-4
100	0.003205754194082824
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3712075.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.98643396955256
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.55110533313133	42.9153758651441
2	11.464445301424577	11.919912573201115
3	2.990162183520615	4.663436067355426
4	1.001196222131608	2.0819448516964125
5	0.45019973443479	1.1702139383652153
6	0.28183364388626936	0.8790915670975159
7	0.2017090082069592	0.7340292425350513
8	0.14022536357361648	0.5831853283421051
9	0.11529973011765685	0.5394619625821918
>10	0.6690652026658283	7.266040568019529
>50	0.09665863280456687	3.2743192452789684
>100	0.03164326143034856	2.9432884419948557
>500	0.001911089271989635	0.6974420279001118
>1k	0.0039771317247845744	5.094409357845882
>5k	2.5825530680419315E-4	0.958089973895369
>10k+	3.099063681650318E-4	14.279758988746192
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	267373	7.202790891886614	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	164484	4.4310527131052035	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	54929	1.4797384212334073	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17922	0.4828027450953981	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	14844	0.399884161823239	No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAGGTATATCTCGTA	12252	0.3300579864361577	TruSeq Adapter, Index 5 (97% over 37bp)
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	9231	0.24867493248385336	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7415	0.199753507135497	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7356	0.19816409959389292	No Hit
GTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGT	6411	0.17270663981735282	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC	5268	0.14191523608763293	No Hit
CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4316	0.11626920253497033	No Hit
TTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTG	4314	0.11621532431322106	No Hit
CCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGAT	4313	0.1161883852023464	No Hit
CACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCA	4122	0.1110430150252891	No Hit
AGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTC	4116	0.1108813803600412	No Hit
ACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGA	4011	0.10805277371820343	No Hit
CCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATT	3911	0.10535886263073888	No Hit
CTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCG	3909	0.10530498440898958	No Hit
CCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTC	3877	0.10444293286100091	No Hit
ATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTA	3841	0.10347312486951368	No Hit
TACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACT	3815	0.10277270798677289	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGTAACGAGTGGAATCACTGCTG	3758	0.10123717866691809	No Hit
TCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGT	3736	0.1006445182276759	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.36076857283325364	0.0	0.0	0.0	0.0
2	0.4488055871715954	0.0	0.0	0.0	0.0
3	0.534121751311598	0.0	0.0	0.0	0.0
4	0.5510933911626247	0.0	0.0	0.0	0.0
5	0.5524134075954823	0.0	0.0	0.0	0.0
6	0.5530868853673484	0.0	0.0	0.0	0.0
7	0.5542722062458328	0.0	0.0	0.0	0.0
8	0.5570199955550467	0.0	0.0	0.0	0.0
9	0.561788218179859	0.0	0.0	0.0	0.0
10-11	0.5636066081638975	0.0	0.0	0.0	0.0
12-13	0.5641588599368278	0.0	0.0	0.0	0.0
14-15	0.5645494770445101	0.0	0.0	0.0	0.0
16-17	0.5649535637076298	0.0	0.0	0.0	0.0
18-19	0.565249893927251	0.0	0.0	0.0	0.0
20-21	0.5656135719240587	0.0	0.0	0.0	0.0
22-23	0.5660715368089275	0.0	0.0	0.0	0.0
24-25	0.5664890930274846	0.0	0.0	0.0	0.0
26-27	0.5669605274677909	0.0	0.0	0.0	0.0
28-29	0.5674454314635345	0.0	0.0	0.0	0.0
30-31	0.5679438050147154	0.0	0.0	0.0	0.0
32-33	0.5684556481213338	2.6939110874645583E-5	0.0	0.0	0.0
34-35	0.5690617781160132	2.6939110874645583E-5	0.0	0.0	0.0
36-37	0.5703683249934336	2.6939110874645583E-5	0.0	0.0	0.0
38-39	0.5714324198729821	2.6939110874645583E-5	0.0	0.0	0.0
40-41	0.5728871318602129	4.040866631196837E-5	0.0	0.0	0.0
42-43	0.5741128614050093	5.3878221749291165E-5	0.0	0.0	0.0
44-45	0.5755810429476775	5.3878221749291165E-5	0.0	0.0	0.0
46-47	0.576604729160914	5.3878221749291165E-5	0.0	0.0	0.0
48-49	0.5781133193698942	5.3878221749291165E-5	0.0	0.0	0.0
50-51	0.5801472222409298	5.3878221749291165E-5	0.0	0.0	0.0
52-53	0.5824639857761494	5.3878221749291165E-5	0.0	0.0	0.0
54-55	0.5854676966386725	5.3878221749291165E-5	0.0	0.0	0.0
56-57	0.5892122330502482	5.3878221749291165E-5	0.0	0.0	0.0
58-59	0.5933473865695063	8.081733262393674E-5	0.0	0.0	2.6939110874645583E-5
60-61	0.5991392954075551	8.081733262393674E-5	0.0	0.0	2.6939110874645583E-5
62-63	0.6067226551187679	8.081733262393674E-5	0.0	0.0	2.6939110874645583E-5
64-65	0.6166362479206373	8.081733262393674E-5	0.0	0.0	2.6939110874645583E-5
66-67	0.6297825340274644	8.081733262393674E-5	0.0	0.0	2.6939110874645583E-5
68-69	0.6470505040981123	8.081733262393674E-5	0.0	0.0	2.6939110874645583E-5
70-71	0.6695177225675666	8.081733262393674E-5	0.0	0.0	2.6939110874645583E-5
72-73	0.6972245981021397	8.081733262393674E-5	0.0	0.0	2.6939110874645583E-5
74-75	0.7318548251314965	8.081733262393674E-5	0.0	0.0	2.6939110874645583E-5
76-77	0.7761966016311632	8.081733262393674E-5	0.0	0.0	2.6939110874645583E-5
78-79	0.8297919627162705	8.081733262393674E-5	0.0	0.0	2.6939110874645583E-5
80-81	0.9003185549860927	8.081733262393674E-5	0.0	0.0	2.6939110874645583E-5
82-83	0.9885037344342451	8.081733262393674E-5	0.0	0.0	2.6939110874645583E-5
84-85	1.0939568839530451	8.081733262393674E-5	0.0	0.0	2.6939110874645583E-5
86-87	1.2235474768155277	8.081733262393674E-5	0.0	0.0	2.6939110874645583E-5
88	1.334644370062566	8.081733262393674E-5	0.0	0.0	2.6939110874645583E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	4205	0.0	86.88461	1
GTGGAAT	118970	0.0	57.013645	6
TGGAATC	119890	0.0	56.580055	7
GGAATCA	120760	0.0	56.254154	8
GAATCAC	121225	0.0	56.038372	9
AGTGGAA	120030	0.0	55.907185	5
AACGTGT	1140	0.0	55.825947	1
ACGAGTG	119550	0.0	55.511852	2
AACGAGT	113000	0.0	55.423035	1
CGAGTGG	121810	0.0	54.850113	3
GAGTGGA	124110	0.0	54.136578	4
AGTACTT	40675	0.0	40.910217	20-21
GAGTACT	40025	0.0	40.605827	20-21
TGATACG	610	0.0	40.06421	3
ATCGAGT	815	0.0	39.911556	1
GTACTTT	40445	0.0	39.380745	22-23
TACTTTT	42385	0.0	38.681538	22-23
GATACGG	705	0.0	38.665348	4
ATCTGCG	1835	0.0	38.16424	94
ATACGGC	730	0.0	37.98296	5
>>END_MODULE