FastQCFastQC Report
Thu 26 May 2016
SRR2132659_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132659_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5111724
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3503886.854595435903817No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1485822.9066905803208467No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT190710.37308352328881605No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147040.2876524632394081No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA92720.18138694499155275No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA85030.1663430967712654No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT67290.13163856264540105No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61250.11982258823050697No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG51530.10080747708600855No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGAGT806850.087.942091
ACGAGTG874050.086.12922
TACGAGT49300.085.9509051
CGAGTGG895500.084.643453
GTGGAAT932200.082.458986
GAGTGGA924400.082.382794
GAATCAC935450.082.1624459
TGGAATC937500.081.987797
GGAATCA939450.081.8276148
AGTGGAA934400.081.419855
AACGTGT15200.049.925221
ATCGAGT8350.042.336261
GAGTACT501100.042.15063520-21
AGTACTT510250.042.13626520-21
TGAGTAC933700.041.58346618-19
ATCACTG951600.041.55202510-11
CGTGTGG19850.041.1971473
GTACTTT505600.041.12821222-23
GCTGAGT964750.040.9174216-17
AATCACT939300.040.91283810-11
Produced by FastQC (version 0.11.5)