FastQCFastQC Report
Thu 26 May 2016
SRR2132658_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132658_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3738645
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2614616.993469559158465No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1341803.5890008278400334No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG289760.7750401549224384No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165270.44205855329939053No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT140750.3764732944689854No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA71580.1914597400930016No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68150.18228529320114642No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT54090.14467808524211312No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC51440.13758995571925123No Hit
CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT37770.10102590644471458No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT37650.087.867791
AACGAGT838250.068.632291
ACGAGTG890950.068.2868652
GTGGAAT910350.068.2641456
TGGAATC919450.067.588527
CGAGTGG909650.067.3480763
GAATCAC925050.067.214919
GGAATCA925450.067.160488
AGTGGAA924100.066.552575
GAGTGGA934750.065.816184
AACGTGT11250.052.3618551
CGTGTGG14000.043.306213
AGTACTT376150.041.63985420-21
GAGTACT371050.041.58517520-21
GTACTTT373750.040.54146222-23
CATGGGG192700.038.87565224-25
ATCGAGT6550.038.8516961
TACTTTT403050.038.41052622-23
ATCTGCG17650.038.3478694
TCGAGTG7000.038.2706032