##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132657_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7040256
Sequences flagged as poor quality	0
Sequence length	100
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.528520269717465	33.0	30.0	34.0	16.0	34.0
2	31.09937266485764	33.0	31.0	34.0	25.0	34.0
3	31.872532476091777	34.0	31.0	34.0	28.0	34.0
4	35.931335593478416	37.0	35.0	37.0	35.0	37.0
5	35.68932294507472	37.0	35.0	37.0	35.0	37.0
6	36.104787240691245	37.0	36.0	37.0	35.0	37.0
7	36.14786891272136	37.0	35.0	37.0	35.0	37.0
8	36.291938105659796	37.0	37.0	37.0	35.0	37.0
9	38.2004231096142	39.0	39.0	39.0	37.0	39.0
10-11	38.242014850028184	39.0	39.0	39.0	37.0	39.0
12-13	38.183240779880734	39.0	39.0	39.0	37.0	39.0
14-15	39.6822723491873	41.0	40.0	41.0	37.5	41.0
16-17	39.390245326306314	41.0	39.5	41.0	36.5	41.0
18-19	38.70990458301516	40.5	37.0	41.0	35.0	41.0
20-21	38.42380497243282	40.0	37.0	41.0	35.0	41.0
22-23	37.26021667393913	40.0	36.5	41.0	33.0	41.0
24-25	37.13401018372059	40.0	36.0	41.0	33.0	41.0
26-27	37.08101431254772	40.0	35.5	41.0	33.0	41.0
28-29	36.951667027449005	40.0	35.0	41.0	33.0	41.0
30-31	36.772186196070145	40.0	35.0	41.0	32.5	41.0
32-33	36.62106058359241	40.0	35.0	41.0	32.0	41.0
34-35	36.50907410185084	40.0	35.0	41.0	31.0	41.0
36-37	36.306727908757864	39.5	35.0	41.0	31.0	41.0
38-39	35.87374407976074	39.0	35.0	41.0	31.0	41.0
40-41	35.7141197706447	39.0	35.0	41.0	30.5	41.0
42-43	35.58360186050052	39.0	35.0	41.0	30.0	41.0
44-45	35.39372474523654	38.5	35.0	41.0	30.0	41.0
46-47	35.268757698583684	38.0	35.0	41.0	29.5	41.0
48-49	35.09021177638995	38.0	35.0	41.0	28.0	41.0
50-51	34.8123068962265	38.0	35.0	41.0	26.5	41.0
52-53	34.251730689906736	37.5	35.0	41.0	21.5	41.0
54-55	33.298669537016835	37.0	34.5	40.5	7.0	41.0
56-57	32.68065848741864	37.0	34.0	40.0	2.0	41.0
58-59	32.343642191420315	36.0	34.0	40.0	2.0	41.0
60-61	32.09321805911603	36.0	33.5	40.0	2.0	41.0
62-63	31.86244321229228	35.0	33.0	40.0	2.0	41.0
64-65	31.578652466614848	35.0	33.0	39.5	2.0	41.0
66-67	31.23736530319352	35.0	33.0	39.0	2.0	41.0
68-69	30.981533697070105	35.0	33.0	39.0	2.0	41.0
70-71	30.71901937940893	35.0	33.0	37.5	2.0	40.0
72-73	30.441423365854877	35.0	32.5	37.0	2.0	40.0
74-75	30.289969569288388	35.0	33.0	37.0	2.0	39.5
76-77	30.034752003336244	35.0	32.5	36.0	2.0	39.0
78-79	29.749527502977166	35.0	32.0	36.0	2.0	39.0
80-81	29.483048201656302	35.0	32.0	35.5	2.0	38.5
82-83	29.1914796138095	35.0	31.5	35.0	2.0	37.0
84-85	28.901343928402603	35.0	31.0	35.0	2.0	37.0
86-87	28.741217648903675	35.0	31.0	35.0	2.0	36.5
88-89	28.540968041503035	35.0	31.0	35.0	2.0	36.0
90-91	28.35504341035326	35.0	31.0	35.0	2.0	36.0
92-93	28.226561931838845	35.0	31.0	35.0	2.0	36.0
94-95	28.03201943224792	35.0	30.0	35.0	2.0	35.0
96-97	27.796035044748372	35.0	29.5	35.0	2.0	35.0
98-99	27.658890742041198	35.0	29.0	35.0	2.0	35.0
100	27.583549234573287	35.0	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	3.280670449208955
1101	2	1.9985807441899155
1101	3	1.2655272391655288
1101	4	0.5628555915792433
1101	5	0.8177959000380213
1101	6	0.39657360362480176
1101	7	0.24508787503264529
1101	8	0.16313751657928321
1101	9	0.2118478896994631
1101	10-11	0.12451956609631765
1101	12-13	0.1567416956381109
1101	14-15	0.1533663381881638
1101	16-17	0.02319582443774948
1101	18-19	-0.05975008068865861
1101	20-21	0.04979582406752314
1101	22-23	-0.4335445925150978
1101	24-25	-0.378459526004292
1101	26-27	-0.34614767025553306
1101	28-29	-0.34979721186430623
1101	30-31	-0.31511242133502293
1101	32-33	-0.3066763324616346
1101	34-35	-0.2985823289209506
1101	36-37	-0.26079887794145407
1101	38-39	-0.23892902106041447
1101	40-41	-0.21239043189037687
1101	42-43	-0.23921027787818616
1101	44-45	-0.20479850340720418
1101	46-47	-0.2131795022473142
1101	48-49	-0.18348564850187898
1101	50-51	-0.2291833899921656
1101	52-53	-0.14938222131756618
1101	54-55	-0.09232646687248547
1101	56-57	-0.10869883625292687
1101	58-59	-0.2604275272262413
1101	60-61	-0.21201995781037297
1101	62-63	-0.14396291033371966
1101	64-65	-0.11042679753320783
1101	66-67	-0.11428030732464833
1101	68-69	-0.2070677992323695
1101	70-71	-0.14092109126161034
1101	72-73	-0.12289989893996278
1101	74-75	-0.11794321490211601
1101	76-77	-0.044069679402731765
1101	78-79	-0.08562109628373804
1101	80-81	-0.06483486073280886
1101	82-83	-0.07327469160798827
1101	84-85	0.002548599991339273
1101	86-87	-0.0781755537719242
1101	88-89	-0.016435220316900256
1101	90-91	0.028112040117040493
1101	92-93	0.04012066934113534
1101	94-95	0.048725732296372115
1101	96-97	0.021914491351946452
1101	98-99	0.04826126819223475
1101	100	0.05312984933961573
1102	1	3.282798990295454
1102	2	1.9690858699916802
1102	3	1.256655062619096
1102	4	0.5625026347500466
1102	5	0.8174739648051386
1102	6	0.38665588438771437
1102	7	0.24563799134065079
1102	8	0.18769283348557053
1102	9	0.16585963700351414
1102	10-11	0.1864210934388666
1102	12-13	0.15595788330452365
1102	14-15	0.15120183502889972
1102	16-17	0.19812855927641237
1102	18-19	0.06649759068096728
1102	20-21	0.019403966156538388
1102	22-23	-0.12515959290458767
1102	24-25	-0.1257894282724692
1102	26-27	-0.05317971464536697
1102	28-29	-0.04634855249130965
1102	30-31	0.0014833054515648314
1102	32-33	0.057026033015517896
1102	34-35	0.06724260311302999
1102	36-37	0.038366618138681474
1102	38-39	0.028781380618703167
1102	40-41	0.051383329216221796
1102	42-43	0.003917691721568417
1102	44-45	-0.07149020182122001
1102	46-47	-0.11519422854377126
1102	48-49	-0.03571301060686238
1102	50-51	0.04983082180537224
1102	52-53	0.09470534966156663
1102	54-55	0.029954001907498196
1102	56-57	-0.03820191393997163
1102	58-59	-0.025553370353023297
1102	60-61	0.03821942550786872
1102	62-63	0.020475792945170213
1102	64-65	0.006937667389067315
1102	66-67	0.012099148381913949
1102	68-69	0.07137527050854331
1102	70-71	0.09702868454416702
1102	72-73	0.06689126137573709
1102	74-75	0.11194542675869457
1102	76-77	0.19629897665209484
1102	78-79	0.16892500376159703
1102	80-81	0.160213492744262
1102	82-83	0.14095712060243315
1102	84-85	0.09795057898535475
1102	86-87	0.1335948671130076
1102	88-89	0.1705900774502105
1102	90-91	0.20949462440903588
1102	92-93	0.153374657856709
1102	94-95	0.1523972646046765
1102	96-97	0.2228189662057254
1102	98-99	0.27167317501622534
1102	100	0.25028817920121327
1103	1	3.2932123792495744
1103	2	1.9925617415255772
1103	3	1.2770111472104873
1103	4	0.551247721388151
1103	5	0.8469325856840797
1103	6	0.4096977642720958
1103	7	0.27677790872655805
1103	8	0.21584237336902845
1103	9	0.19938196794954877
1103	10-11	0.15142506130442968
1103	12-13	0.17381010773439698
1103	14-15	0.2222613553358883
1103	16-17	0.21094885054494483
1103	18-19	0.040997192829465234
1103	20-21	-0.03581459754873606
1103	22-23	-0.22360633593898882
1103	24-25	-0.22518746671308776
1103	26-27	-0.1846804962621178
1103	28-29	-0.1651097022806809
1103	30-31	-0.11798921384826855
1103	32-33	-0.13326879144915438
1103	34-35	-0.04489576904651926
1103	36-37	-0.008228559178974137
1103	38-39	-0.03398735308470435
1103	40-41	-0.03544065603496449
1103	42-43	0.017556693502051246
1103	44-45	0.05091487847953147
1103	46-47	-0.032691983216892595
1103	48-49	0.005088623125608649
1103	50-51	0.030055453242120223
1103	52-53	3.6758143855308845E-4
1103	54-55	0.021642511923396057
1103	56-57	-0.04616539156961608
1103	58-59	-0.0813675253467494
1103	60-61	-0.07618069779270087
1103	62-63	-0.049949928969653
1103	64-65	-0.06348701379000943
1103	66-67	-0.07245111968741469
1103	68-69	-0.014667528805365038
1103	70-71	-0.03417462575066921
1103	72-73	-0.049440356147385955
1103	74-75	0.02987588063275126
1103	76-77	0.06905122219680493
1103	78-79	0.05486918973246091
1103	80-81	0.03960130970759934
1103	82-83	0.0802081161508923
1103	84-85	0.07111971906945058
1103	86-87	0.0520042046522704
1103	88-89	0.07431185499589787
1103	90-91	0.1004876857511654
1103	92-93	0.04736656296562103
1103	94-95	0.057633385280070115
1103	96-97	0.11068595262514336
1103	98-99	0.15884870144215313
1103	100	0.1738730423918966
1104	1	2.4597077932139584
1104	2	1.7173113571718446
1104	3	1.1724881289003264
1104	4	0.5065933344561202
1104	5	0.8255678174282153
1104	6	0.35810340910217064
1104	7	0.23753413946207047
1104	8	0.1526386632970329
1104	9	0.14942295224072666
1104	10-11	0.15102190030096807
1104	12-13	0.12448106349498289
1104	14-15	0.10065583677083367
1104	16-17	0.08552079079255748
1104	18-19	0.06884851000064884
1104	20-21	-0.031353674903918716
1104	22-23	-0.07876551800428899
1104	24-25	-0.04971390086086558
1104	26-27	-0.013036268710884258
1104	28-29	0.03392597162146416
1104	30-31	0.07085692426792178
1104	32-33	0.04977428209043211
1104	34-35	0.0685190547671084
1104	36-37	0.06193700814096559
1104	38-39	0.0460892699709845
1104	40-41	0.06812834705311843
1104	42-43	0.0762185779960447
1104	44-45	0.06634801302609361
1104	46-47	0.05301601969318881
1104	48-49	-0.004355476662929902
1104	50-51	-0.03281995048051556
1104	52-53	2.928995924307287E-4
1104	54-55	-0.06523148399026724
1104	56-57	-0.09591775774279654
1104	58-59	-0.07458003134462388
1104	60-61	-0.11348026214379558
1104	62-63	-0.22231606183249042
1104	64-65	-0.09324264603390375
1104	66-67	-0.10265420937878389
1104	68-69	-0.12532413574383483
1104	70-71	-0.13908963014862863
1104	72-73	-0.13258293827869494
1104	74-75	-0.07016392280393902
1104	76-77	-0.029930478014769335
1104	78-79	0.0553269004530641
1104	80-81	0.004001052569329033
1104	82-83	0.05240907339186052
1104	84-85	0.018332873673259087
1104	86-87	-0.009279378417385686
1104	88-89	0.01968783042490685
1104	90-91	0.09158827669683234
1104	92-93	0.019535464327404384
1104	94-95	0.028294335458955544
1104	96-97	0.041729263487678026
1104	98-99	0.06637241501639224
1104	100	0.04302002191012022
1105	1	3.231219530313709
1105	2	1.9376281688915853
1105	3	1.2335449032337706
1105	4	0.5642729271652911
1105	5	0.7872634132562126
1105	6	0.3492011967642412
1105	7	0.18596684908496997
1105	8	0.15020932154943978
1105	9	0.12744341827183092
1105	10-11	0.08428035243027665
1105	12-13	0.08903048705576566
1105	14-15	0.1403633600380303
1105	16-17	0.0641904519583818
1105	18-19	0.018552505481181925
1105	20-21	-0.030216984218718324
1105	22-23	-0.22559631475790098
1105	24-25	-0.21349514138719172
1105	26-27	-0.204765490862016
1105	28-29	-0.1888527914024749
1105	30-31	-0.11972530845776674
1105	32-33	-0.10914182418433427
1105	34-35	-0.10874096164721436
1105	36-37	-0.10190251389262528
1105	38-39	-0.052672699739851225
1105	40-41	-0.05405420317336507
1105	42-43	-0.06137320016384251
1105	44-45	-0.06405264167418778
1105	46-47	0.007007511810257938
1105	48-49	-0.0012039685247771104
1105	50-51	0.023543921988128602
1105	52-53	-0.09125200319589055
1105	54-55	0.05057734442690531
1105	56-57	0.11374173032741908
1105	58-59	0.15165466121644045
1105	60-61	0.11875075020926573
1105	62-63	0.15992914405711645
1105	64-65	0.21280922141794179
1105	66-67	0.18908614477429353
1105	68-69	0.1212465335563131
1105	70-71	0.109636125446535
1105	72-73	0.15118925246371262
1105	74-75	0.17478645505683943
1105	76-77	0.2019401533298364
1105	78-79	0.17589773528205654
1105	80-81	0.17277431718947156
1105	82-83	0.14477945766033073
1105	84-85	0.1733116311171372
1105	86-87	0.18866859758991694
1105	88-89	0.2152541665769121
1105	90-91	0.2818460897424906
1105	92-93	0.2219054572437429
1105	94-95	0.2561435802523313
1105	96-97	0.3096381735645082
1105	98-99	0.35756637418731785
1105	100	0.30937871879366696
1106	1	1.7436254614407503
1106	2	1.3313335104771582
1106	3	0.9608836469373117
1106	4	0.40633271148008987
1106	5	0.6397913027150253
1106	6	0.22049602438023896
1106	7	0.1319515878233446
1106	8	0.1011834005888943
1106	9	0.11633016914087335
1106	10-11	0.10242582582026927
1106	12-13	0.038995575731789245
1106	14-15	0.0625984713798644
1106	16-17	0.08695054717206574
1106	18-19	0.061897942726844235
1106	20-21	0.04227019246228281
1106	22-23	-0.20510859663188086
1106	24-25	-0.23945178726486915
1106	26-27	-0.18490612256707095
1106	28-29	-0.23133186260825056
1106	30-31	-0.16909295358951226
1106	32-33	-0.16955115878295146
1106	34-35	-0.21418407715932375
1106	36-37	-0.15921936579301388
1106	38-39	-0.19082573680004344
1106	40-41	-0.19045413196470662
1106	42-43	-0.1769924581105613
1106	44-45	-0.08097071067386707
1106	46-47	-0.20105560001542244
1106	48-49	-0.17086796300664275
1106	50-51	-0.09734721588163353
1106	52-53	-0.08337827112765694
1106	54-55	-0.12141264064015189
1106	56-57	-0.04070440993276492
1106	58-59	-0.029850491151364622
1106	60-61	0.0074080709492037045
1106	62-63	-0.04542888466539097
1106	64-65	-0.05477405275484415
1106	66-67	-0.056717773286067086
1106	68-69	-0.008590975335771134
1106	70-71	0.024734881562991973
1106	72-73	-0.0338233944536519
1106	74-75	-0.031689627041199486
1106	76-77	-0.045467075609245455
1106	78-79	-0.02552770694604689
1106	80-81	0.04524908275529427
1106	82-83	0.011838998972873327
1106	84-85	0.03601104596771165
1106	86-87	-0.010983234179082757
1106	88-89	0.0696499603698797
1106	90-91	0.07796516372749807
1106	92-93	0.05737647841482385
1106	94-95	0.09197993281368255
1106	96-97	0.10495709512409945
1106	98-99	0.05563964530727361
1106	100	0.011445137970035546
1107	1	2.7991378667376594
1107	2	1.7785760724644462
1107	3	1.1637187081707836
1107	4	0.4805723377799467
1107	5	0.7875674038270333
1107	6	0.36139018417996027
1107	7	0.16551314507943715
1107	8	0.1254082900080462
1107	9	0.08199053363002662
1107	10-11	0.1082098371481024
1107	12-13	0.10399311567709901
1107	14-15	0.1103888570472833
1107	16-17	0.18790470682900207
1107	18-19	0.18461597909400496
1107	20-21	0.21626613878769518
1107	22-23	-0.07731163396213248
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2216	18-19	-0.32109611096069557
2216	20-21	-0.1626193161365066
2216	22-23	0.7812069805429331
2216	24-25	0.7415131784675495
2216	26-27	0.7070875859905499
2216	28-29	0.6091803644332927
2216	30-31	0.6199219322167764
2216	32-33	0.5291375499814635
2216	34-35	0.5547706852846659
2216	36-37	0.5284431087380099
2216	38-39	0.5259716647161667
2216	40-41	0.44535979069181053
2216	42-43	0.45064141535949886
2216	44-45	0.4900921050879248
2216	46-47	0.4730340614470805
2216	48-49	0.4354088482495868
2216	50-51	0.47130118537147325
2216	52-53	0.5038960505964525
2216	54-55	0.6285628106634604
2216	56-57	0.7068532789587465
2216	58-59	0.6685619299377663
2216	60-61	0.6296558919156539
2216	62-63	0.6255830258357733
2216	64-65	0.5744305168499011
2216	66-67	0.4872841961531478
2216	68-69	0.46235457971982896
2216	70-71	0.5100824007883666
2216	72-73	0.522432641115472
2216	74-75	0.5454959123331342
2216	76-77	0.5080130419554187
2216	78-79	0.6106100591857988
2216	80-81	0.6212260679280988
2216	82-83	0.5204991608656506
2216	84-85	0.5450425759925785
2216	86-87	0.4910676213768568
2216	88-89	0.41120628169734985
2216	90-91	0.36570246623545444
2216	92-93	0.4102324486826987
2216	94-95	0.3583379134430551
2216	96-97	0.31964784009894487
2216	98-99	0.2825865507981078
2216	100	0.20250123425611122
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1113.0
3	487.0
4	930.0
5	3245.0
6	11574.0
7	23688.0
8	92414.0
9	131817.0
10	15188.0
11	25141.0
12	18896.0
13	24908.0
14	24512.0
15	25114.0
16	29176.0
17	34094.0
18	42942.0
19	68035.0
20	142510.0
21	184378.0
22	82784.0
23	45949.0
24	42468.0
25	43782.0
26	46683.0
27	52195.0
28	61579.0
29	72796.0
30	84430.0
31	103274.0
32	134783.0
33	189122.0
34	298924.0
35	1035751.0
36	743629.0
37	1224297.0
38	1371406.0
39	499580.0
40	6662.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	12.210531893949991	48.109363465756715	25.210481465579505	14.469623174713792
2	13.78806486335384	47.293269437003794	23.500650695993297	15.418015003649069
3	15.060752138490615	28.877982388683577	21.419137910805958	34.642127562019844
4	32.19776439214812	30.307722071450023	22.434941550164243	15.059571986237605
5	13.775625140797477	46.09666071707072	23.295793021104068	16.831921121027733
6	34.577109127849894	27.71389279026217	24.215511481400675	13.493486600487255
7	15.678762249554563	27.66129527108105	41.9219556788844	14.737986800479982
8	34.246013213155884	28.183023458237884	23.3036554352569	14.267307893349331
9	33.65778176248136	29.79110418712047	23.306141140322172	13.244972910075997
10-11	13.484474144122032	48.870623454601656	23.63590897830988	14.008993422966437
12-13	13.260270649249117	29.07095281762481	33.323021208319695	24.34575532480637
14-15	13.571649951805634	38.51003985374366	23.711521564173506	24.2067886302772
16-17	23.882004273992916	28.48225667962311	33.29658513789629	14.33915390848769
18-19	14.064098952090587	28.80653976265492	33.54254685785196	23.586814427402526
20-21	23.91913006986193	38.776144603521175	23.79879332298663	13.505932003630273
22-23	23.625722488136145	29.25060805408347	32.713849457129825	14.40982000065056
24-25	13.862841067143014	38.89580861832297	22.646527342187557	24.59482297234646
26-27	14.491319633831498	33.84314434020581	37.16406051143595	14.501475514526744
28-29	24.542765206265226	29.002418377968077	32.0847210669612	14.370095348805497
30-31	20.86403534189666	31.24127872622814	32.6150426916294	15.27964324024581
32-33	16.232031635783674	32.464276332016645	33.671810632440824	17.631881399758857
34-35	17.035681468593403	32.47118612144123	33.538756639290625	16.95437577067475
36-37	17.462665409515054	31.77568593405863	34.328306904836104	16.433341751590213
38-39	18.44926637465019	31.566954658660883	33.69692405459926	16.28685491208967
40-41	15.71163148837425	34.124642209362996	33.91198657491905	16.251739727343704
42-43	15.611612013135625	31.943930632512924	35.07463910871832	17.369818245633123
44-45	16.017225165690377	32.960707097113996	33.3766490825605	17.64541865463513
46-47	17.275543906510617	31.69375827174321	34.358556608996324	16.672141212749857
48-49	16.038013905468496	31.964502533199774	34.3723207087164	17.62516285261533
50-51	17.158139586415043	33.32627150543209	32.97449162651391	16.541097281638955
52-53	17.515300464367094	32.23167859277275	33.333461170111335	16.919559772748823
54-55	16.786428293941892	33.881170159802906	30.752077167878628	18.580324378376574
56-57	17.33484654093455	34.62889897457222	29.918656805723394	18.117597678769844
58-59	19.55345419360484	34.21163984639064	28.2417918478406	17.993114112163923
60-61	18.58563534812924	35.292391401771084	27.86416700555859	18.257806244541094
62-63	17.273163337283005	35.18262582451001	27.785266472374104	19.75894436583288
64-65	18.349681020705336	35.167762860962355	28.307124643857296	18.175431474475015
66-67	18.250324296127374	33.94019883533924	29.482997579275445	18.32647928925794
68-69	19.259290981658157	34.097916534391366	28.654331577569575	17.988460906380904
70-71	16.534413996347364	36.2806044923224	28.77128203379984	18.4136994775304
72-73	16.617488715064532	34.26444393262539	29.605844168710803	19.51222318359927
74-75	16.969994221791175	35.453337980899526	28.014568813239997	19.5620989840693
76-77	18.364665534923887	34.01106037925622	28.721518195323142	18.90275589049675
78-79	17.849024191594175	34.539624789814624	28.012493654312216	19.598857364278985
80-81	18.99269904925498	35.673545180860444	27.011365801715755	18.322389968168824
82-83	18.46443838514846	35.23799655366789	27.818219510603225	18.479345550580426
84-85	17.95758355568235	36.08946686731522	26.447619414899115	19.50533016210332
86-87	17.96201282603179	36.05388686236239	27.422953146481582	18.56114716512424
88-89	20.48249622714689	34.90094205384827	26.546944349413394	18.069617369591455
90-91	18.903471198206923	36.06126511212207	26.614373289852033	18.420890399818976
92-93	17.628616981955965	36.22665373822868	26.590185088222952	19.554544191592406
94-95	18.337632636827227	36.47570988224265	26.455801929004675	18.730855551925455
96-97	18.65000246090971	35.09359517886126	27.316921634194614	18.939480726034414
98-99	19.933979288984784	35.170602034170834	25.92071406910158	18.974704607742805
100	17.800020085121712	37.3392781884527	26.008755351711933	18.851946374713656
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	809995.0
1	447061.0
2	55542.5
3	21084.0
4	14010.0
5	12452.0
6	12263.0
7	13311.0
8	13134.5
9	12055.5
10	12213.5
11	14797.5
12	76780.5
13	82433.0
14	24471.0
15	21213.0
16	23966.5
17	26196.0
18	26919.0
19	28996.5
20	32094.0
21	35448.0
22	39595.5
23	44499.0
24	49845.0
25	55575.5
26	62251.5
27	69960.5
28	76909.0
29	83132.5
30	90189.0
31	97665.0
32	106152.5
33	116340.5
34	128242.0
35	137422.5
36	144916.0
37	151289.5
38	154276.5
39	156447.0
40	157215.5
41	158633.5
42	157511.0
43	153496.0
44	155090.0
45	159250.5
46	166217.5
47	180064.0
48	186517.0
49	217992.0
50	358185.0
51	359251.5
52	207102.0
53	160052.0
54	157836.5
55	164735.5
56	156626.0
57	131340.5
58	109818.0
59	85058.5
60	65757.5
61	54169.5
62	43681.0
63	32651.5
64	23069.0
65	16214.5
66	11973.5
67	9125.0
68	6832.5
69	4826.5
70	3576.5
71	2642.0
72	1956.5
73	1570.0
74	1172.5
75	838.0
76	673.0
77	511.5
78	356.0
79	204.0
80	107.5
81	74.0
82	56.0
83	35.0
84	18.0
85	8.0
86	6.0
87	1.0
88	3.0
89	3.0
90	1.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2897337824079124
2	0.0014488109523290063
3	1.420402894440202E-4
4	0.0028692138467692084
5	2.8408057888804044E-5
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	1.988564052216283E-4
16-17	1.4204028944402022E-5
18-19	7.102014472201011E-6
20-21	1.7755036180502526E-4
22-23	0.0024857050652703537
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	7.102014472201011E-6
34-35	0.0022584406021599213
36-37	2.8408057888804044E-5
38-39	1.988564052216283E-4
40-41	7.102014472201011E-6
42-43	1.7755036180502526E-4
44-45	0.001299668648412785
46-47	0.001399096851023599
48-49	0.002102196283771499
50-51	8.238336787753173E-4
52-53	4.0481482491545764E-4
54-55	2.982846078324425E-4
56-57	3.337946801934475E-4
58-59	8.522417366641213E-4
60-61	0.0022016244863823134
62-63	1.988564052216283E-4
64-65	9.303638958583324E-4
66-67	1.2073424602741718E-4
68-69	2.1306043416603033E-4
70-71	0.0018962378640776697
72-73	1.0653021708301516E-4
74-75	1.7044834733282428E-4
76-77	0.0011576283589687648
78-79	5.96569215664885E-4
80-81	0.0020666862114104944
82-83	2.4857050652703536E-4
84-85	0.001214444474746373
86-87	0.006143242518453875
88-89	0.0022655426166321227
90-91	0.004190188538598596
92-93	0.006789525835424166
94-95	0.00505663430420712
96-97	0.0025070111086869565
98-99	5.823651867204829E-4
100	0.0031106823388240426
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	7040256.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.56045667480219
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.02635918359664	26.324111385416337
2	13.499704242955593	9.601112957085313
3	5.780255754628005	6.166456029951756
4	2.670120966645255	3.798028838034781
5	1.2557971918152462	2.2328360815942156
6	0.6365996677837698	1.3582664943250926
7	0.3758717440401744	0.9356319608456074
8	0.2410437246001024	0.6857299940299912
9	0.17115429394932788	0.5477692369222289
>10	1.164274615589812	7.5474649662901445
>50	0.06771652186435498	1.6964073017836194
>100	0.09553926127434212	6.6222884982209616
>500	0.009305613322451407	2.244209643037302
>1k	0.004372030480287991	3.5127978448614963
>5k	0.00144397336963357	3.4665272739280364
>10k+	4.412140851658131E-4	23.260361493673155
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	748343	10.629485632340643	No Hit
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	521265	7.404063147703719	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	118944	1.689484018762954	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	79580	1.130356623395513	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	41194	0.5851207683356969	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32446	0.460863923130068	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	28938	0.41103618959310567	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC	19383	0.27531669302934436	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	15047	0.21372802352641723	No Hit
TCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12898	0.1832035653248973	No Hit
GTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGT	12731	0.18083149249118216	No Hit
ACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGA	9682	0.13752340823970038	No Hit
AGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTC	8389	0.11915759881458857	No Hit
CCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGAT	8383	0.11907237464092214	No Hit
TACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACT	8076	0.11471173775499073	No Hit
TCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGT	8073	0.11466912566815751	No Hit
TTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTG	7934	0.11269476564488563	No Hit
ACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTC	7877	0.11188513599505472	No Hit
CACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCA	7801	0.11080562979528018	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGTAACGAGTGGAATCACTGCTG	7760	0.11022326460855968	No Hit
CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7693	0.10927159466928475	No Hit
CCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTC	7642	0.10854718919312024	No Hit
TCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACT	7569	0.1075102950801789	No Hit
TCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCA	7382	0.10485414166757573	No Hit
ATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTA	7368	0.10465528526235411	No Hit
CCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATT	7139	0.10140256263408602	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.00948829133486055	0.0	0.0	0.0	0.0
2	0.00954510745063816	0.0	0.0	0.0	0.0
3	0.00961612759536017	0.0	0.0	0.0	0.0
4	0.00963033162430457	0.0	0.0	0.0	0.0
5	0.00963033162430457	0.0	0.0	0.0	0.0
6	0.00963033162430457	0.0	0.0	0.0	0.0
7	0.009658739682193374	0.0	0.0	0.0	0.0
8	0.00968714774008218	0.0	0.0	0.0	0.0
9	0.009729759826915384	0.0	0.0	0.0	0.0
10-11	0.009843392058470601	0.0	0.0	0.0	0.0
12-13	0.009992534362386823	0.0	0.0	0.0	0.0
14-15	0.010141676666303043	0.0	0.0	0.0	0.0
16-17	0.010411553216246682	0.0	0.0	0.0	0.0
18-19	0.010603307606996109	0.0	0.0	0.0	0.0
20-21	0.010851878113523145	0.0	0.0	0.0	0.0
22-23	0.011143060706883385	0.0	0.0	0.0	0.0
24-25	0.011370325169993818	0.0	0.0	0.0	0.0
26-27	0.011554977546271045	0.0	0.0	7.102014472201011E-6	0.0
28-29	0.011732527908076069	0.0	0.0	1.4204028944402022E-5	0.0
30-31	0.011910078269881094	0.0	0.0	1.4204028944402022E-5	0.0
32-33	0.012201260863241338	0.0	0.0	1.4204028944402022E-5	0.0
34-35	0.01249954547107378	0.0	0.0	1.4204028944402022E-5	0.0
36-37	0.012925666339405839	0.0	0.0	1.4204028944402022E-5	0.0
38-39	0.013614561743209339	0.0	0.0	1.4204028944402022E-5	0.0
40-41	0.014523619595651068	0.0	0.0	1.4204028944402022E-5	0.0
42-43	0.015709656012508635	0.0	0.0	2.130604341660303E-5	0.0
44-45	0.01692410048725501	0.0	0.0	2.8408057888804044E-5	0.0
46-47	0.01845813561325043	0.0	0.0	2.8408057888804044E-5	0.0
48-49	0.01994955865241264	0.0	0.0	2.8408057888804044E-5	0.0
50-51	0.02227191738482237	0.0	0.0	2.8408057888804044E-5	0.0
52-53	0.0252902735355078	0.0	0.0	2.8408057888804044E-5	0.0
54-55	0.028628220337442275	0.0	0.0	3.551007236100505E-5	0.0
56-57	0.033052775353623505	0.0	0.0	4.261208683320606E-5	0.0
58-59	0.03850712246827388	0.0	0.0	4.261208683320606E-5	0.0
60-61	0.04503387376822661	0.0	0.0	4.261208683320606E-5	0.0
62-63	0.05379775962692265	0.0	0.0	5.681611577760809E-5	7.102014472201011E-6
64-65	0.06426612895894694	0.0	0.0	5.681611577760809E-5	1.4204028944402022E-5
66-67	0.07761081415221265	0.0	0.0	5.681611577760809E-5	1.4204028944402022E-5
68-69	0.09540846241954838	0.0	0.0	7.102014472201011E-5	1.4204028944402022E-5
70-71	0.11841898930947965	0.0	0.0	7.102014472201011E-5	1.4204028944402022E-5
72-73	0.14647194647467365	0.0	0.0	7.812215919421111E-5	1.4204028944402022E-5
74-75	0.1813783476055416	0.0	0.0	8.522417366641213E-5	1.4204028944402022E-5
76-77	0.22378447601905385	0.0	0.0	9.942820261081415E-5	1.4204028944402022E-5
78-79	0.27873986400494527	0.0	0.0	9.942820261081415E-5	1.4204028944402022E-5
80-81	0.34907821533762406	0.0	0.0	1.1363223155521618E-4	1.4204028944402022E-5
82-83	0.4351404267117559	0.0	0.0	1.2073424602741719E-4	1.4204028944402022E-5
84-85	0.5439006763390422	0.0	0.0	1.6334633286062326E-4	1.4204028944402022E-5
86-87	0.6747694970182903	0.0	0.0	2.0595841969382931E-4	1.4204028944402022E-5
88	0.7868747954619832	0.0	0.0	2.2726446311043235E-4	1.4204028944402022E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	7885	0.0	87.75607	1
AACGTGT	2315	0.0	59.86171	1
ATCTGCG	4595	0.0	57.69176	94
CGTGTGG	2565	0.0	56.433132	3
GTGGAAT	236765	0.0	55.002274	6
TGGAATC	239125	0.0	54.49088	7
GGAATCA	241480	0.0	54.006176	8
GAATCAC	241880	0.0	53.91336	9
AGTGGAA	241805	0.0	53.31554	5
ACGAGTG	239505	0.0	53.168156	2
AACGAGT	226580	0.0	53.12734	1
CGAGTGG	243595	0.0	52.608482	3
GAGTGGA	247435	0.0	52.08644	4
ACGTGTG	2845	0.0	50.879807	2
TCCGATC	1420	0.0	43.355534	7
ATCGAGT	1425	0.0	41.678154	1
CGATCTA	825	0.0	41.5847	9
AGTACTT	78455	0.0	41.093246	20-21
GAGTACT	77655	0.0	40.430176	20-21
TCGAGTG	1570	0.0	39.813374	2
>>END_MODULE